KR102014960B1

KR102014960B1 - Composition for anti oxidation containing extract of soybean root

Info

Publication number: KR102014960B1
Application number: KR1020120083738A
Authority: KR
Inventors: 강영규; 박준성; 심진섭; 황경환; 박준호; 염명훈; 조준철
Original assignee: (주)아모레퍼시픽
Priority date: 2012-07-31
Filing date: 2012-07-31
Publication date: 2019-08-28
Also published as: KR20140017198A

Abstract

The present invention relates to an antioxidant, anti-aging, skin regeneration, whitening or moisturizing composition comprising soy root extract. The composition of the present invention not only prevents oxidation of the body, prevents aging, but also proliferates and regenerates skin cells, and is useful as it has a skin whitening function. In addition, the composition of the present invention has a skin moisturizing function. In particular, the composition of the present invention shows a better effect than the soybean extract in antioxidant capacity, anti-aging, skin regeneration, whitening or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.

Description

Antioxidant Composition Containing Soy Root Extract {COMPOSITION FOR ANTI OXIDATION CONTAINING EXTRACT OF SOYBEAN ROOT}

The present invention relates to an antioxidant, anti-aging, skin regeneration, whitening or moisturizing composition comprising soy root extract.

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of UV rays reaching the surface of the sun's rays, not only skin thickness is reduced, wrinkles are increased, elasticity is decreased, but blemishes, freckles, and brown mushrooms also cause various changes.

As aging progresses, symptoms such as changes and decreases in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are constituting skin, appear, and are subjected to oxidative stress caused by free radicals and active harmful oxygen. In addition, by aging or ultraviolet light, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation in most cells that make up the skin Increased biosynthesis, increased biosynthesis of matrix metalloproteinase (MMP), an enzyme that degrades skin tissue by these inflammatory factors, and increased nitric oxide (NO) production by inducible nitric oxide synthase (iNOS) It is known. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight. As a result, decomposition and degeneration are accelerated, and the skin matrix is destroyed and thinned, resulting in various symptoms of skin aging. Therefore, a lot of research is being conducted on the active ingredient that can prevent and improve the phenomenon of aging.

On the other hand, free radicals generated by various physical, chemical and environmental factors such as enzymes, reducing metabolism, chemicals, pollutants and photochemical reactions in the body are non-selective and irreversible to cell components such as lipids, proteins, sugars and DNA. It has been known to cause various diseases including cell aging or cancer by performing a destructive action. In addition, various peroxides in the body, including lipid peroxides produced as a result of lipid peroxidation by these free radicals, also cause oxidative destruction of cells and cause various functional disorders. Accordingly, antioxidants such as free radical scavengers or peroxide production inhibitors are expected as agents for inhibiting or treating aging and various diseases caused by these oxides.

Therefore, the present inventors confirmed that when the soybean root extract was applied to the skin while developing a material using soybeans, the skin's antioxidant function, anti-aging function, skin regeneration function, and whitening function can be improved as well. The present invention has been completed.

Republic of Korea Patent Publication No. 1998-077876

An object of the present invention is to provide a composition comprising a natural extract that can be used for antioxidant, anti-aging, skin regeneration, whitening or moisturizing.

In order to achieve the above object, the present invention provides an antioxidant composition comprising soybean root extract as an active ingredient.

The present invention also provides an anti-aging composition comprising a bean root extract as an active ingredient, a composition for skin regeneration comprising the bean root extract as an active ingredient and a whitening composition comprising a bean root extract as an active ingredient.

The present invention also provides a moisturizing composition comprising soybean root extract as an active ingredient.

The composition of the present invention not only prevents oxidation of the body, prevents aging, but also proliferates and regenerates skin cells, and is useful as it has a skin whitening function. In addition, the composition of the present invention has a skin moisturizing function. In particular, the composition of the present invention shows a better effect than the soybean extract in antioxidant capacity, anti-aging, skin regeneration, whitening or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.

In the present specification, 'bean' is not limited in kind. In the composition which is one aspect of the present invention, specifically, the soybean of the present specification is selected from the group consisting of frosted, seomoktae, heuktae, chungtae, yellow, hedge bean, kidney bean, zebra kidney bean, red bean, soybean, bean sprout bean and soybean It may include any one or more, but is not limited thereto.

In the present specification, 'bean root' refers to the root portion of the bean tree.

In the present specification, 'soybean root extract', for example, in the extraction process, washed and dried soybean root or powdered soybean root powder into water or an organic solvent, extracted and deposited, and then filtered through filter cloth and centrifugation. The residue and the filtrate are separated, and the filtrate is concentrated under reduced pressure to obtain a soybean root extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, preferably water or 30 to 70% concentration. Ethanol is used. After obtaining the extract using a solvent in the above can be obtained by cooling, heating and filtration at room temperature in a conventional manner known in the art to obtain a liquid, or may further evaporate the solvent, spray drying or freeze drying, but The present invention is not limited thereto, and any method generally used in the art may be used without limitation.

The present invention relates to an antioxidant composition comprising soybean root extract as an active ingredient.

As used herein, the term "antioxidant" refers to an effect that can slow down, prevent, or prevent the oxidation process known in the art, and is not limited thereto.

In another aspect, the present invention relates to an anti-aging composition comprising a soybean root extract as an active ingredient.

As used herein, 'anti-aging' refers to an effect that can slow down, prevent or prevent the aging process known in the art, and specifically, by effectively inhibiting the expression of collagenase in the skin, it reduces collagen breakdown in the skin, thereby making skin elasticity. It may mean, but is not limited to, the effect of improving the appearance and improving wrinkles.

In another aspect, the present invention relates to a composition for skin regeneration comprising a soybean root extract as an active ingredient. The composition of the present invention can promote the growth of skin cells to regenerate skin.

In another aspect, the present invention relates to a whitening composition comprising soybean root extract as an active ingredient. Specifically, the composition of the present invention can provide an excellent whitening effect by inhibiting the production of melanin.

The present invention relates to a moisturizing composition comprising soybean root extract as an active ingredient in another aspect. Specifically, the composition of the present invention may promote differentiation of keratinocytes to provide an excellent moisturizing effect.

In one aspect of the present invention, the composition may comprise 0.001 to 10% by weight of the bean root extract based on the total weight of the composition. If the content of soybean root extract is less than 0.001% by weight based on the total weight of the composition, the antioxidant, anti-aging, whitening and moisturizing effects are insignificant. In view of the above, the composition of the present invention, 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to the total weight of the composition It may include 7% by weight, 0.1-6.5% by weight, 0.3-6% by weight, 0.5-5.5% by weight or 0.7-5% by weight of the bean root extract.

In the composition of one aspect of the invention, the soybean is Seoritae ( Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR), yellow bean, Glycime max MERR), field bean, Vicia faba ), Kidney bean ( Phaseolus) vulgaris , pinto bean, Phaseolus vulgaris L.), small red bean, Vigna angularis ), Soybeans (small black bean, Phaseolus angularis WF WIGHT.), Sprouting bean, Glycine max (L.) Merr.) and soybean ( Glycine) max ) may include any one or more selected from the group consisting of, specifically soybean (soybean, Glycine max ), but is not limited thereto.

In a composition of one aspect of the present invention, the composition may inhibit the generation of reactive oxygen species (ROS).

In one aspect of the present invention, the composition may increase the expression of collagenase (Collagenase) or the activity of collagenase.

In a composition which is one aspect of the present invention, the composition may proliferate skin cells.

In one aspect of the present invention, the composition may inhibit the expression of tyrosinase or the activity of tyrosinase.

In a composition which is one aspect of the present invention, the composition may promote differentiation of keratinocytes.

In the composition which is one aspect of this invention, the said composition contains a cosmetic composition.

Cosmetic compositions according to the invention may be provided in all formulations suitable for topical application. For example, it may be provided in the form of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a foam, or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may include other ingredients in addition to the above-mentioned substances within the range not impairing the main effect, preferably giving a synergistic effect to the main effect. In addition, the cosmetic composition according to the present invention may further include a moisturizer, an emulsifier, a UV absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavors, coolants or limiting agents. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.

In a composition that is one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, the composition may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier.

Examples of preparations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets and the like. In addition, preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspensions, and other commonly used auxiliaries can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

The active ingredient of the pharmaceutical composition of the present invention will depend on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of one skilled in the art and its daily dosage may be, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

In a composition which is one aspect of the present invention, the composition comprises a health food composition.

The composition according to the invention provides various types of food additives or functional foods comprising. Fermented milk, cheese, yogurt, juice, probiotic, tablets, granules, drinks, caramels, diet bars and the like containing the composition, and can be processed into conventional tea leaf form or tea bags and dietary supplements. And other various food additives.

In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, it may further include additives such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers and synthetic polymer materials to improve physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the amount of the additives may be selected within a range that does not impair the object and effect of the present invention. For example, the addition amount of the components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The formulations of the compositions according to the invention may be in various forms, such as solutions, emulsions, viscous mixtures, tablets, powders, and the like, which may be administered by various methods such as simple drinking, injection, spray or squeeze.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

[ Example 1] Preparation of Soybean Root Extract

The soybean root was washed with purified water, dried and then granulated. 100 g of the soybean root powder was added to 1 liter of an aqueous 70% ethanol solution, and extracted by boiling for 12 hours in an extractor equipped with a cooling condenser, followed by filtration with a 300 mesh filter cloth. Thereafter, the filtrate was After aging at 7 ° C. for 7 days, the filter was filtered using Whatman No. 2 filter paper. The extract was added to a 3-liter separatory funnel, 1 liter of ethyl acetate was added, shaken and mixed, and when the two layers were completely separated, the upper layer (ethyl acetate layer) was taken. The lower layer (water layer) is again extracted twice with a separatory funnel. Each separated upper layer was combined and concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser and dried.

[ Comparative example 1] Preparation of Soybean Extract

The beans were washed with purified water, dried and then granulated. 100 g of the soy flour was added to 1 liter of an aqueous 70% ethanol solution, and extracted by boiling for 12 hours in an extractor equipped with a cooling condenser, followed by filtration with a 300 mesh filter cloth. Thereafter, the filtrate was After aging at 7 ° C. for 7 days, the filter was filtered using Whatman No. 2 filter paper. The extract was added to a 3-liter separatory funnel, 1 liter of ethyl acetate was added, shaken and mixed, and when the two layers were completely separated, the upper layer (ethyl acetate layer) was taken. The lower layer (water layer) is again extracted twice with a separatory funnel. Each separated upper layer was combined and concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser and dried.

[ Example 2] active Oxygen species ( ROS : reactive oxygen species ) Inhibitory effect

The keratinocytes (keratinocyte) isolated from the skin tissue of the person into a 5 × 10 ⁴ are in each well of a 24-well plate was attached for 24 hours. After 16 hours, the soybean root extract was treated with 1%. At this time, 1% of soybean extract was treated for comparison, and the control group did not process soybean root extract. After 2 hours, the culture solution was removed, and 100 µl of phosphate buffered saline (PBS) was added to each well. The keratinocytes were irradiated with UV 30mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: K5T8, UV B 15 W, Sankyo Dennki, Japan), and then PBS was removed and each cell was treated with keratinocyte culture solution 200. Μl was added. The soybean root extract and soybean extract were retreated and the amount of reactive oxygen species increased by UV stimulation at certain time points was quantified. The amount of ROS was quantified by referring to Tan's method for measuring the fluorescence of DCF-DA (dichlorofluorescin diacetate) oxidized by ROS (Tan et al., 1998, J. Cell Biol. Vol. 141, pp1423-1432). Table 1 shows the results of calculating the ratio of the vehicle-treated control group ROS.

Test substance UVB 30 mJ / cm ² Time since survey 0hr 2hr 3hr Vehicle 100 243 288 UVB + Vehicle 100 324 383 UVB + Soybean Extract 100 289 358 UVB + Soybean Root Extract 100 245 290

In the results of Table 1, the soybean root extract according to the present invention inhibits the production of ROS known to cause skin cell damage by ultraviolet rays more effectively than the soybean extract, and the amount of ROS after UV stimulation is almost the same as the case of not irradiated with ultraviolet rays. It can be seen that the antioxidant effect is excellent enough to inhibit the production of ROS to a similar level.

Therefore, it was confirmed that the soybean root extract according to the present invention can prevent pores from widening by inhibiting oxidation and preventing aging, and can improve skin trouble by defending the skin against irritation.

[ Example 3] Collagenase Generation suppression effect

Inhibition of collagenase production of soybean root extract and soybean extract prepared in Example 1 and Comparative Example 1 was measured in comparison with tocopherol (Comparative Example 1) and EGCG (Comparative Example 2). Tocopherols and EGCG are known substances that have the function of regenerating the epidermal cells of the skin to prevent aging of the skin.

Place human fibroblasts at 5,000 cells / well in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum, 90% Incubate until growth. After culturing for 24 hours in serum-free DMEM medium, 100 ug / ml concentration of the soybean root extract of Example 1, 100 ug / ml concentration of the soybean extract of Comparative Example 1, tocopherol and EGCG respectively dissolved in serum-free DMEM medium 10 After treatment for 24 hours at ^-4 molarity, the cell culture was collected.

The degree of collagenase production of the cell cultures collected using the collagenase measuring instrument (Amersham Pharmacia, USA) was measured. First, the collected cell culture was placed in a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours.

After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, add the coloring inducing substance and color it for 15 minutes at room temperature. Then, add 1M sulfuric acid to stop the reaction (color development). The color of the reaction solution becomes yellow, and the degree of yellow varies depending on the progress of the reaction. It was confirmed.

The absorbance of the yellowish 96-well plate (96-well plate) was measured at 405 nm using an absorbance meter, and the degree of synthesis of collagenase was calculated by the following equation (1). At this time, the reaction absorbance of the collected cell culture medium of the group not treated with the composition was used as a control. That is, the expression level of collagenase in the non-treated group was set to 100, and the collagenase expression level in the group treated with the test substance was calculated. The results are shown in Table 2 below.

[ Equation One]

Collagenase expression level (%) = absorbance of substance treated cell group / absorbance of control group X 100

Test substance Collagenase expression level (%) Untreated group 100 Tocopherol (Comparative Example 1) 76 EGCG (Comparative Example 2) 59 Bean extract 77 Soybean Root Extract 61

As can be seen in Table 2, the soybean root extract inhibited the expression of collagenase more effectively than the soybean extract in vitro , and was superior to the collagenase expression inhibiting ability than tocopherol, which is known as an antioxidant. I could confirm it.

[ Example 4] effect of increasing cell proliferation

In order to see the skin cell proliferation effect as skin regeneration effect, human normal fibroblasts were inoculated to 1 × 10 ⁴ cells in each well of a 96-well microplate. Incubated for 24 hours in DMEM (Dulbecco's Modified Eagle's Medium) medium. After incubation, the soybean root extract and the soybean extract were adjusted to a final concentration of 100 μg / ml, and then incubated for 24 hours after replacing with DM medium without serum. 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium (MTT: (3- (4,5-dimdimeth-thiazol-2-yl) 2,5-diphenyl tetrazolium: 5 mg / ml) was added 10 µl and left for 4 hours, after which the media portion was discarded, and 100 µl of dimethyl sulfoxide solution was added to each well, and the absorbance was measured at 570 nm using a microplate reader.

By performing the test of the above procedure to obtain the cell proliferation effect from the following equation 2 and the results are shown in Table 3 below.

[ Equation 2]

Cell proliferation effect (%) = [(absorbance-control absorbance at extract treatment) / control absorbance] X100

Test substance
Concentration (μg / ml) Absorbance (570nm) Cell proliferation effect (%) 0 (control) 0.300 - Bean Extract 100 0.383 27.7 Bean Root Extract 100 0.465 55.0

As shown in Table 3, the soybean root extract was found to have a cell proliferation effect of normal fibroblasts compared to the soybean extract.

[ Example 5] Tyrosinase Inhibitory effect

Tyrosinase enzyme was extracted from the mushroom (Mushroom) was used as the Sigma (SIGMA). First, the substrate tyrosine was dissolved in distilled water to make a solution of 0.3 mg / ml, and the solution was added to the test tube by 1.0 ml. Then, 1.0 ml of potassium-phosphate buffer solution (0.1 mol, pH 6.8) and 0.7 ml of distilled water Added.

The soybean root extract of the present invention and the soybean extract of Comparative Example 1 were mixed with an ethanol solution at a final concentration of 1 mg / ml, and 0.2 ml of the sample solution was added to the reaction solution, followed by reaction for 10 minutes in a 37 ° C thermostat. In this case, the control group was prepared by adding only 0.2 ml of solvent instead of each sample solution, and ascorbic acid was used as a positive control group. 0.1 ml of a tyrosinase solution of 2500 units / ml was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostat. The reaction tube containing the reaction solution was placed in iced water, quenched to stop the reaction, and the absorbance at 475 nm was measured with a photospectrometer. The results are shown in Table 2 below. Each tyrosinase inhibitory effect was calculated by the following equation.

[ Equation 3]

Test substance Tyrosinase inhibition rate (%) Control group (no addition) 0 Ascorbic acid 52 Bean extract 29 Soybean Root Extract 59

As Table 4, soybean root extract according to the present invention is Tyrosinase Suppression beans Than extract Great Just Well known Tyrosinase It is much higher than the inhibitor of ascorbic acid, which shows that the whitening effect is very excellent.

[ Example 6] skin moisturizing test

When human keratinocytes cultured in primary culture were put in a culture flask and attached to the bottom, and then the soybean root extract and soybean extract were added to the culture at a concentration of 1 mg / ml, and the cells grew about 80 to 90% of the floor area. Incubated for 5 days. The cells were harvested and washed with PBS (phosphate buffered saline) and then 10 mM Tris-HCl (Tris-HCl, pH 7.4) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). 1 ml was added and sonicated for 3 minutes, and then boiled for 10 minutes. Thereafter, the mixture was centrifuged at 1200 rpm for 30 minutes, the precipitate was separated, suspended in 1 ml of PBS, and the absorbance was measured at 340 nm.

Separately, a portion of the solution after the sonication was taken to measure the protein content, which was used as a reference for evaluating the degree of cell differentiation. Low calcium (0.03mM) treated group and high calcium (1.2mM) treated group as a negative / positive control, respectively, and the test results performed by adding the test substance to the low calcium concentration is shown in Table 5 below.

Test substance Concentration Differentiation capacity in keratinocytes (%) Control Low Ca ² ⁺ (0.03 mM) 100 Control High Ca ² ⁺ (1.2 mM) 206 Bean extract 1 mg / ml 136 Soybean Root Extract 1 mg / ml 187

As shown in Table 5, as a result of measuring the amount of CE (Cornified Envelop) produced when keratinocyte differentiation was compared to compare the effect of promoting cell differentiation, Soybean root extract according to the present invention was found to promote differentiation in keratinocytes compared to soybean extract.

[ Example 7] Irritation exam

To compare the usability of kojic acid, a known whitening substance, and the soybean root extract used as an active ingredient in the present invention, the degree of irritation such as stinging, burning, etc. was tested in 15 panels sensitive to irritation such as stinging, burning, etc. It was.

The subjects were rubbed with kojic acid (purchased through YM chemical) and the soybean root extract obtained in Example 1 by applying randomly changing the left and right sides, respectively, and scoring a score between 0 and 3.0 in units of 0.1 points. . The results are shown in Table 6 below.

0 to 0.4: no stimulation

0.5 to 1.0: slightly irritating

1.1 to 2.0: moderate stimulation

2.1 to 3.0: severe irritation

division Kojic acid Soybean Root Extract Stinging 0.81 0.22 Burning 0.39 0.18 Average 0.60 0.20

As can be seen in Table 6, in the case of kojic acid, there was some degree of tingling, burning, there was a sense of irritation that can usually be felt slightly. On the other hand, the soybean root extract used in the present invention was almost irritable because it is hardly felt both hot and stinging.

Hereinafter, the formulation examples of the composition according to the present invention, but the pharmaceutical composition and cosmetic composition is applicable to a variety of formulations, which is intended to explain in detail only, not intended to limit the present invention.

Preparation Example 1 Soft Capsule

Soybean root extract 150 mg, palm oil 2 mg, palm hardened oil 8 mg, lead 4 mg and lecithin 6 mg were mixed, and 400 mg per capsule was filled according to a conventional method to prepare a soft capsule.

Preparation Example 2 Tablet

150 mg of soybean root extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added to form granules, and dried at 60 ° C. using a tablet press. Tableting.

Preparation Example 3 Granule

150 mg of soybean root extract, 100 mg of glucose, 50 mg of red ginseng extract, and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C. to form granules, followed by filling into sachets. The final weight of the content was 1 g.

[Example 4] Drinks

150 mg of soy root extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled into bottles. The final dose of the contents was 200 ml.

Preparation Example 5 Preparation of Health Food

Bean Root Extract .............. 1000 mg

Vitamin mixtures

Vitamin A Acetate ......... 70 μg

Vitamin E ......................................... 1.0 mg

Vitamin B1 ..................... 0.13 mg

Vitamin B2 ......................................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ......................... 0.2 μg

Vitamin C ......................................... 10 mg

Biotin ... 10 ㎍

Nicotinic Acid Amide ...................................... 1.7 mg

Folic Acid ... ..... 50 ㎍

Calcium Pantothenate ............... 0.5 mg

Mineral mixture

Ferrous Sulfate ......................................... 1.75 mg

Zinc Oxide ......................................... 0.82 mg

Magnesium Carbonate ......................................... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dibasic Calcium Phosphate ......................................... 55 mg

Potassium Citrate ......................................... 90 mg

Calcium Carbonate ......................................... 100 mg

Magnesium Chloride ......................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Preparation Example 6 Preparation of Healthy Drinks

Soybean Root Extract ...................................... 1000 mg

Citric Acid ... .... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ..................... ..... 2 g

Taurine ... ........... 1 g

Purified water is added to the whole ........... 900 ㎖

After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained by sterilization in a sterilized 2 L container sealed sealed sterilized and then stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose. Those skilled in the art to which the present invention pertains will be able to perform various applications and modifications within the scope of the present invention based on the above contents.

[Formulation Example 1] Lotion

Ingredient weight% Soybean Root Extract 3.00 L-ascorbic acid-2-magnesium phosphate 1.00 Water-soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 Baekgulchae Extract 0.20 1,3-butylene glycol 3.00 Purified water to 100

Formulation Example 2 Cream

Ingredient weight% Soybean Root Extract 1.00 Polyethylene Glycol Monostearate 2.00 Self-emulsifying glycerin monostearate 5.00 Cetyl alcohol 4.00 Squalene 6.00 Tri2-ethylhexaneglyceryl 6.00 Sphingolipid 1.00 1,3-butylene glycol 7.00 Purified water to 100

[Formulation Example 3] Pack

Ingredient weight% Soybean Root Extract 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-magnesium phosphate 1.00 Lauroylhydroxyproline 1.00 Water-soluble collagen (1% aqueous solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water to 100

Formulation Example 4 Serum

Ingredient weight% Soybean Root Extract 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 Dark glycerin 4.00 Sodium Hyaluronate (1% aqueous solution) 5.00 Purified water to 100

Formulation Example 5 Ointment

Compounding ingredient Content (% by weight) Soybean Root Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 Beeswax 4.0 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 6 Ointment

As described above in detail the specific parts of the present invention, it is apparent to those skilled in the art that such specific description is merely a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims

Antioxidant cosmetic composition comprising soybean root extract as an active ingredient.

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Cosmetic composition for skin regeneration comprising soybean root extract as an active ingredient.

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The composition of claim 1 or 3, wherein the composition comprises from 0.001 to 10% by weight of soybean root extract based on the total weight of the composition.

According to claim 1 or 3, wherein the soybean (Seoritae, Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR, yellow bean, Glycime max MERR, field bean, Vicia faba , kidney bean (kidney bean, Phaseolus vulgaris ), pinto bean ( Phaseolus vulgaris L.), red (small red) bean, Vigna angularis ), soybeans (small black bean, Phaseolus angularis WF WIGHT.), bean sprouts (sprouting bean, Glycine max (L.) Merr.) and soybeans (soybean, Glycine max ) The above composition.

The composition of claim 1 or 3, wherein the composition inhibits the production of Reactive Oxygen Species (ROS).

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The composition of claim 3, wherein the composition propagates skin cells.

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