KR102063686B1 - Skin external composition containing extract of soybean root - Google Patents

Abstract

The present invention relates to an external composition for skin containing soybean extract of a specific stage of the growth stage of soybean. The composition according to the present invention is not only excellent in anti-oxidant ability, excellent in anti-aging ability, but also has a skin moisturizing effect and a skin whitening effect, and is useful because it contains components derived from nature and has no skin irritation.

Description

Skin external composition containing soybean root extract {SKIN EXTERNAL COMPOSITION CONTAINING EXTRACT OF SOYBEAN ROOT}

The present invention relates to an external composition for skin containing soybean extract of a specific stage of the growth stage of soybean.

As human skin ages, it changes with a variety of internal and external factors. That is, internally, the secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells is reduced, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays that reach the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness is reduced, wrinkles are increased, elasticity is reduced only In addition, the color of the skin becomes dull, skin problems frequently occur, and various changes such as blemishes, freckles, and black mushrooms also increase.

As aging progresses, symptoms of alteration or decrease in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are constituting skin, appear, and are subject to oxidative stress caused by free radicals and active harmful oxygen. In addition, by aging or ultraviolet light, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation in most cells that make up the skin. Increased biosynthesis, increased biosynthesis of matrix metalloproteinase (MMP), an enzyme that breaks down skin tissues by these inflammatory factors, and increased nitric oxide (NO) production by inducible nitric oxide synthase (iNOS) It is known. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight. As a result, decomposition and degeneration are accelerated, and the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, a lot of research is being conducted on the active ingredients that can prevent and improve the phenomenon of aging.

On the other hand, free radicals generated by various physical, chemical and environmental factors such as enzymes, reducing metabolism, chemicals, pollutants and photochemical reactions in the body are non-selective and irreversible to cell components such as lipids, proteins, sugars and DNA. It has been known to cause various diseases including cell aging or cancer by performing a destructive action. In addition, various peroxides in the body, including lipid peroxides produced as a result of lipid peroxidation by these free radicals, can cause oxidative destruction of cells and cause various functional disorders. Accordingly, antioxidants such as free radical scavengers or peroxide production inhibitors are expected as agents for suppressing or treating aging and various diseases caused by these oxides.

Accordingly, the present inventors have confirmed that when the soybean root extract is applied to the skin, it is possible to enhance not only the antioxidant and anti-aging functions of the skin but also the whitening and moisturizing functions, thereby completing the present invention.

Republic of Korea Patent Publication No. 1998-077876

It is an object of the present invention to provide a composition for external application of skin derived from nature that promotes skin health.

In order to achieve the above object, the present invention provides a skin external preparation composition comprising a soybean root extract.

The composition according to the present invention is not only excellent in anti-oxidant ability, excellent in anti-aging ability, but also has a skin moisturizing effect and a skin whitening effect, and is useful because it contains components derived from nature and has no skin irritation.

Figure 1 shows the cumestrol content in soybean root extract according to the growth time.
Figure 2 is a comparison of the content of cumestrol present in the soybean root extract of V4 season grown by treatment with an induction factor and the soybean root extract of V4 grown without treatment.

The present invention relates to a topical skin composition comprising soybean root extract in one aspect. In the extraction process of the soybean root extract of the present specification, for example, washed and dried fine powdered soybean root powder in water or an organic solvent, extracted and deposited, and then separating the residue and the filtrate through filter cloth filtration and centrifugation. The concentrated filtrate was concentrated under reduced pressure to obtain a soybean root extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, preferably water or 30 to 70% concentration. Ethanol is used. After obtaining the extract using the solvent in the above, it is possible to obtain a liquid by cooling, heating and filtration at room temperature in a conventional manner known in the art, or may further evaporate the solvent, spray drying or freeze drying, but The present invention is not limited thereto, and any method generally used in the art may be obtained without any limitation.

In the external preparation composition for skin, which is an aspect of the present invention, the soybean root extract may be extracted from the soybeans of V3 to V4 during the soybean growth stage.

In this specification, the growth stage of the beans is as follows.

VE period: 1-2 weeks after planting seeds, with cotyledons from soil

VC season: Cotyledon blooms wide, a word grows over it, and leaves are formed.

Period V1: A word is produced from the first outer leaf, resulting in three leaves.

V2 phase: One more word on the V1 Stage, resulting in 3 leaves

V3 phase: Three more leaves are created in the V2 Stage.

V4 season: 3 more leaves are created in V3 Stage.

R2 season: Beanstalk in full bloom

R4 season: Soybean pod creation complete

R5 season: Soybeans in the pod

R6 Season: Green Soybeans Completed

R7 season: Soybean pods and beans turning yellow

R8 season: Soybean pods and soybeans completely yellow as leaves fall

In the composition of one aspect of the present invention, the soybean comprises soybean treated with the inducer in the growth stage of the soybean. Soybean treated with the inducer is increased in the amount of cumestrol may have a better effect in the desired effect of the present invention, that is, antioxidant capacity, anti-aging, skin moisturizing effect and skin whitening effect.

In the present specification, the inducer means a factor that can be processed during the growth process of soybean to enhance the content of cumetrol of soybean root. Specifically, the yeast may include Aspergillus oryzae or chitosan, but is not limited thereto. The inducer may be treated in the soil in which the soybean grows, may be directly processed into the soybean root, or injected by injection, but is not limited thereto. The inducer may be absorbed by the soybean to enhance the content of the cumetrol of the soybean root. Any path can be used without limitation.

In the composition of one aspect of the present invention, the soybean root extract is at least 0.2% by weight based on the total weight of the extract of cumestrol (coumestrol) It may include. Soybean root extract of the present specification is a material extracted from the soybean treated the inducer, it may include the cumestrol in the above range. Specifically, the soybean root extract may contain at least 0.24% by weight, at least 0.28% by weight, at least 0.32% by weight, at least 0.36% by weight, at least 0.4% by weight, at least 0.44% by weight, or at least 0.48% by weight. have.

In one aspect of the present invention, the soybean includes soybean treated with the inducer until the V4 stage of the soybean growth stage. Regular induction of inducers from VE to V4 can significantly increase the amount of cumestrol.

In one aspect of the present invention, the inducer may be at least one selected from the group consisting of Nuruk bacteria ( Aspergillus oryzae ) and chitosan.

In a topical skin composition according to one aspect of the present invention, the soybean root extract may contain at least 0.12% by weight of coumestrol with respect to the total weight of the extract. Soybean root extract of the present specification is a material from which the soybean extract of V3 to V4 stage, it may include the cumestrol in the above range. Specifically, the soybean root extract may contain at least 0.13% by weight, at least 0.14% by weight, at least 0.15% by weight, at least 0.16% by weight, at least 0.17% by weight, at least 0.18% by weight, or at least 0.19% by weight. have.

In an external composition for external application of the present invention, the soybean root extract may contain coumestrol in an amount of 0.12 to 0.2% by weight based on the total weight of the extract. If the cumestrol (coumestrol) is contained less than 0.12% by weight, the effect of the present invention is not fully exhibited, and when contained in excess of 0.2% by weight, it was confirmed that the content ratio of other active ingredients is excessively reduced. In the above-described aspect, the external composition for skin preparations in one aspect of the present invention is 0.125 to 0.195% by weight, 0.13 to 0.19% by weight, 0.135 to 0.185% by weight, 0.14 to 0.18% by weight or 0.145 to 0.175% by weight of soybean root extract It may include.

In an external preparation composition for skin, which is an aspect of the present invention, the soybean is Seoritae (Glycin ) max MERR), Seomokktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr.), Cheongtae (blue bean, Glycime) max MERR), yellow bean, Glycime max MERR, field bean, Vicia faba ), Kidney bean, Phaseolus vulgaris ), pinto bean, Phaseolus vulgaris L., small red bean, Vigna angularis ), Soybeans (small black bean, Phaseolus angularis WF WIGHT.), Sprouting bean, Glycine max (L.) Merr.) and soybean (Soybean, Glycine max ) may comprise one or more beans selected from the group consisting of cousins, and the beans specifically include soybeans ( Glycine max ).

In an external preparation composition for skin, which is an aspect of the present invention, the composition may include 0.001 to 10% by weight of soybean root extract based on the total weight of the composition. This is because if the content of the extract is less than 0.001% by weight, the antioxidant, anti-aging, whitening, and moisturizing effects are insignificant. In view of the above, the composition of the present invention is 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to the total weight of the composition. It may include 7% by weight, 0.1-6.5% by weight, 0.3-6% by weight, 0.5-5.5% by weight or 0.7-5% by weight of the bean root extract.

In the external preparation composition for skin, which is an aspect of the present invention, the composition may be an external preparation composition for antioxidant that inhibits oxidation of DPPH (1,1-diphenyl-2-picryl hydrazyl).

In the external preparation composition for skin, which is an aspect of the present invention, the composition may be an external preparation composition for anti-aging that inhibits the expression of collagenase.

In the external preparation composition for skin, which is an aspect of the present invention, the composition may be a skin preparation composition for whitening which inhibits the production of melanin.

In the external preparation composition for skin, which is an aspect of the present invention, the composition may be an external preparation composition for moisturizing for enhancing skin barrier function or inducing differentiation of keratinocytes.

In a topical skin composition according to one aspect of the present invention, the composition includes a cosmetic composition.

When formulating the external composition for skin according to the present invention in the form of cosmetics, such as softening cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack It may be formulated in a form, the formulation is not particularly limited. In addition, the composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type or a ratio. With ionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics It may contain adjuvants commonly used in the same cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological fields. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

In the external preparation composition for skin, which is an aspect of the present invention, the composition may be an external preparation pharmaceutical composition for skin.

The formulation of the pharmaceutical composition according to the present invention may be, but is not limited to, solutions, suspensions, emulsions, gels, drops, suppositories, patches or sprays. The formulations can be readily prepared according to conventional methods in the art, and include excipients, hydrating agents, emulsifiers, suspending agents, salts or buffers for controlling osmotic pressure, colorants, spices, stabilizers, preservatives, preservatives or other compatible agents. Adjuvants may be used as appropriate.

The active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of one of skill in the art, and its daily dosage may be, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Preparation of Soybean Root Extract

The soybean roots collected at each growth period (VE ~ R8, 12 total) were washed with purified water, dried, and then granulated. 100 g of the soybean root powder was put in 1 liter of an aqueous 70% ethanol solution, extracted by boiling for 12 hours in an extractor equipped with a cooling condenser, and filtered through a 300 mesh filter cloth. Thereafter, the filtrate was poured into 4-15 After aging at 7 ° C. for 7 days, the filter was filtered using Whatman No. 2 filter paper. The extract was added to a 3-liter separatory funnel, 1 liter of ethyl acetate was added, shaken and mixed, and when the two layers were completely separated, the upper layer (ethyl acetate layer) was taken. The lower layer (water layer) is again extracted twice with a separatory funnel. Each separated upper layer was combined and concentrated under reduced pressure to 50 ° C. using a distillation apparatus equipped with a cooling condenser and dried.

Within the Root Extracts by Growth Period Kumestrol  Content analysis

HPLC was used for the quantitative analysis of the coumestrol content in the soybean root extract for each growth period obtained in Example 1 above. Cumestrol has a structure represented by the following Chemical Formula 1, and in this embodiment, cumestrol purchased from Indofine Chemical Company was used.

Kumestrol content in the soybean root extract according to the growth time is shown in FIG.

In soybean root extract obtained by induction factor treatment during soybean growth Kumestrol  Content analysis

Yeast ( Aspergillus) until V4 during Soybean Growth oryzae ) was grown on soil and soybeans. Yeast Bacteria ( Aspergillus oryzae ) dry cells were purchased from Mediogen. One day before processing in soybeans, 10 g of sugar was dissolved in 1 L of water, and 10 g of the dried cells of Aspergillus oryzae were mixed well. Thereafter, the mixture was incubated at a temperature of 30 degrees in a shaking thermostat for 12 hours. After incubation, water solubility was applied to soybeans and the soil around the soybeans. At this time, the control group was sprayed with the same amount of purified water. Yeast Bacteria ( Aspergillus oryzae ) was treated every 5 days. Bean roots collected at the time of V4 was extracted in the same manner as in Example 1 to obtain a soybean root extract, the results of analyzing the cumestrol content in the soybean root extract by the method of Example 2 was as shown in FIG. As can be seen in Figure 2 by treating the induction factor was able to increase the amount of cumestrol in the soybean root extract about 1.6 times.

Antioxidant activity  exam

In order to determine the antioxidant effect of the soybean root extract of each of the VE / V2 / V4 / R4 / R8 step prepared in Example 1, the organic radical DPPH (1,1-diphenyl-2-picrylhydrazyl; 1 The effect of DPPH oxidation inhibition was compared through the change of absorbance caused by the reduction of, 1-diphenyl-2-picryl hydrazyl).

190 μl of 100 μM (in ethanol) DPPH solution and 10 μl of the Root Root Extract (positive control), which is a synthetic root antioxidant and the root extract of VE / V2 / V4 / R4 / R8, were obtained. After making the reaction for 30 minutes at 37 ℃ absorbance was measured at 540nm. The analysis results are shown in Table 1 below, and IC ₅₀ means the sample concentration when the absorbance was reduced by 50% by the added sample.

Test substance IC ₅₀ (ppm) Concentration ratio to show similar efficacy with soybean root extract of V4 season Trolox (positive control) 46 VE Season Bean Root Extract 95 5.2x V2 Season Bean Root Extract 72 3.4x V4 Season Bean Root Extract 49 1x R4 Season Bean Root Extract 77 2.5x R8 Sigi Bean Root Extract 91 3.9 times

As can be seen in Table 1, it can be seen that among the soybean root extract obtained in each step of VE / V2 / V4 / R4 / R8 showed the best antioxidant activity in the soybean root extract of the V4 step. In addition, it was confirmed that the soybean root extract of V4 shows a similar efficacy effect as trolox.

Anti-aging  exam

Inhibition of collagenase production of the soybean root extract of each step VE / V2 / V4 / R4 / R8 prepared in Example 1 was measured in comparison with tocopherol (Comparative Example 1) and EGCG (Comparative Example 2) . Tocopherols and EGCG are known to have the function of regenerating the epidermal cells of the skin to prevent aging of the skin.

Human fibroblasts were placed at 5,000 cells / well in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal bovine serum, and 90% Incubate until growth. After culturing in serum-free DMEM medium for 24 hours, 100 μg / ml concentration of soybean root extract of Example 1, VE / V2 / V4 / R4 / R8, dissolved in serum-free DMEM medium, tocopherol and EGCG, respectively After 24 hours of treatment at 10 ^-4 molarity, the cell culture was collected.

The degree of collagenase production of the cell cultures collected using a collagenase measuring instrument (Amersham Pharma Co., Ltd.) was measured. First, the collected cell culture solution was put into a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours.

After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, the coloring agent is added and color is developed at room temperature for 15 minutes, and 1M sulfuric acid is added to stop the reaction (color development). The color of the reaction solution becomes yellow, and the degree of yellow color varies depending on the progress of the reaction. It was confirmed.

The absorbance of the yellow 96-well plate (96-well plate) was measured at 405nm using an absorbance meter, the degree of synthesis of collagenase was calculated by the following equation (1). At this time, the reaction absorbance of the collected cell culture medium of the group not treated with the composition was used as a control. That is, the expression level of collagenase in the untreated group was set to 100, and the collagenase expression level in the group treated with the test substance was calculated. The results are shown in Table 2 below.

Test substance Collagenase expression level (%) V4 season bean root extract and
To show similar efficacy
Concentration ratio Untreated group 100 - Tocopherol (Comparative Example 1) 76 - EGCG (Comparative Example 2) 59 - VE Season Bean Root Extract 84 4.9 times V2 Season Bean Root Extract 77 3.2 times V4 Season Bean Root Extract 55 1x R4 Season Bean Root Extract 72 2.7 times R8 Sigi Bean Root Extract 81 4.1x

As can be seen in Table 2, V4 season soybean root extract effectively inhibited the expression of collagenase in vitro ( in vitro ), it was confirmed that the ability to inhibit the expression of collagenase better than tocopherol known as antioxidants Could.

Whitening efficacy test

The melanin production inhibitory activity of the soybean root extract of the VE / V2 / V4 / R4 / R8 phase prepared in Example 1 was measured in comparison with hydroquinone (positive control).

Mel-Ab cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) in DMEM with 10% fetal placental serum, 100nM 12-O-tetradecanoyl Incubated at 37 ° C. and 5% CO ₂ in a medium to which phorbol (tetradecanoylphorbol) -13-acetate and 1 nM cholera toxin were added. The cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, the cells were incubated at a concentration of 10 ⁵ cells / well in 24-well plates, and then soybeans of each period for 3 consecutive days from the second day. Root extract and hydroquinone were added by 10ppm and incubated. Thereafter, the culture solution was removed, washed with PBS (phosphate buffered saline), the cells were dissolved with 1N sodium hydroxide, the absorbance was measured at 400 nm, and then the melanin production inhibition rate was calculated according to Equation 2 below. Table 3 (Dooley's method).

Test substance Melanin production inhibition rate (%) Concentration ratio to show similar efficacy with soybean root extract of V4 season Untreated group 100 Hydroquinone (positive control) 48.5 VE Season Bean Root Extract 82.3 5.6 times V2 Season Bean Root Extract 64.8 2.7 times V4 Season Bean Root Extract 47.8 1x R4 Season Bean Root Extract 62.1 2.4x R8 Sigi Bean Root Extract 81.3 5.2x

As shown in Table 3, among the soybean root extracts of the VE / V2 / V4 / R4 / R8 season of Example 1 of the present invention, the V4 seasoned soybean root extract having the highest cumestrol content is hydroquid, a known whitening substance. It was confirmed that the melanin production inhibition rate similar to the rice paddy.

Skin moisturizing test

Human keratinocytes in primary culture were placed in a culture flask and attached to the bottom, and then, each of the root extracts of the VE / V2 / V4 / R4 / R8 phase was added to the culture at a concentration of 10 mg / ml, and the cells were bottomed. It was incubated for 5 days until it grew about 80-90% of the area. The cells were harvested and washed with PBS (phosphate buffered saline) and then 10 mM Tris-HCl (Tris-HCl, pH 7.4) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). 1 ml was added and sonicated for 3 minutes, and then boiled for 10 minutes. Thereafter, the mixture was centrifuged at 1200 rpm for 30 minutes, the precipitate was separated, suspended in 1 ml of PBS, and the absorbance was measured at 340 nm.

Separately, a portion of the solution after the sonication was taken to measure the protein content, which was used as a reference for evaluating the degree of cell differentiation. Low calcium (0.03mM) treated group and high calcium (1.2mM) treated group as a negative / positive control, respectively, the test results performed by adding the test substance to the low calcium concentration is shown in Table 4 below.

Test substance Concentration In keratinocytes
Differentiation capacity (%) Concentration ratio to show similar efficacy with V4 soybean root extract Control Low Ca ²⁺ (0.03 mM) 100 Control High Ca ²⁺ (1.2 mM) 206 VE Season Bean Root Extract 10 mg / ml 118 5.8 times V2 Season Bean Root Extract 10 mg / ml 134 3.9 times V4 Season Bean Root Extract 10 mg / ml 182 1x R4 Season Bean Root Extract 10 mg / ml 156 3.1x R8 Sigi Bean Root Extract 10 mg / ml 129 4.7x

As shown in Table 4, as a result of measuring the amount of CE (Cornified Envelop) produced when keratinocyte differentiation was compared to compare the effect of promoting cell differentiation, Among the root extracts of the VE / V2 / V4 / R4 / R8 phase according to the present invention, the V4 season root extract having the highest cumestrol content was found to promote differentiation in keratinocytes.

Irritation  exam

In order to compare the usability of kojic acid, a known whitening substance, and the soybean root extract used as an active ingredient in the present invention, the degree of irritation such as stinging, burning, etc. was tested in 15 panels sensitive to irritation such as stinging, burning, etc. It was.

Subject subjects rubbed kojic acid (obtained from YM chemical) and V4 seasoned soybean root extract obtained in Example 1 by applying randomly changing the left and right sides, respectively, and scoring a score between 0 and 3.0 in units of 0.1 points. It was made. The results are shown in Table 5 below.

<Evaluation Criteria>

0 to 0.4: no stimulation

0.5 to 1.0: slightly irritating

1.1 to 2.0: moderate stimulation

2.1 to 3.0: severe irritation

division Kojic acid V4 Season Bean Root Extract Stinging 0.81 0.24 Burning 0.39 0.22 Average 0.60 0.23

As can be seen in Table 5, in the case of kojic acid, there was some degree of tingling, burning, there was a sense of irritation that can usually be felt slightly. On the other hand, the soybean root extract of the V4 season used in the present invention was almost irritable because it can hardly feel both hot and stinging.

Hereinafter, the formulation example of the external preparation composition for skin, but not intended to limit the present invention is intended to be described in detail only.

Formulation Example 1 Lotion Ingredient weight% Soybean Root Extract 3.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Water-soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 Baekgulchae Extract 0.20 1,3-butylene glycol 3.00 Purified water to 100

Formulation Example 2 Cream Ingredient weight% Soybean Root Extract 1.00 Polyethylene Glycol Monostearate 2.00 Self-emulsifying glycerin monostearate 5.00 Cetyl alcohol 4.00 Squalene 6.00 Tri2-ethylhexaneglyceryl 6.00 Sphingolipid 1.00 1,3-butylene glycol 7.00 Purified water to 100

Formulation Example 3 Pack Ingredient weight% Soybean Root Extract 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Lauroylhydroxyproline 1.00 Water-soluble collagen (1% aqueous solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water to 100

Formulation Example 4 Serum Ingredient weight% Soybean Root Extract 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 Dark glycerin 4.00 Sodium Hyaluronate (1% aqueous solution) 5.00 Purified water to 100

Formulation Example 5 Ointment Compounding ingredients Content (% by weight) Soybean Root Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 Beeswax 4.0 Preservative, coloring, flavoring Appropriate Purified water Remaining amount

As described above in detail specific parts of the present invention, it is apparent to those skilled in the art that these specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (18)

Contains soybean root extract,
The beans are beans of the V3 to V4 stage of the soybean growth stage,
The soybean root extract is a cosmetic composition comprising the cumestrol (coumestrol) in an amount of 0.12 to 0.2% by weight based on the total weight of the extract.
delete delete delete The method of claim 1,
The soybean is treated with an inducer during the soybean growth stage,
The inducer is to include one or more selected from the group consisting of Nuruk ( Aspergillus oryzae ) and chitosan, cosmetic composition.
The method of claim 1,
The soybean is treated with an inducer until the V4 stage of the soybean growth stage,
The inducer is to include one or more selected from the group consisting of Nuruk ( Aspergillus oryzae ) and chitosan, cosmetic composition.
delete The method of claim 1,
The bean is soybean, cosmetic composition.
The method of claim 1,
The cosmetic composition comprises a 0.001 to 10% by weight of the soybean root extract with respect to the total composition of the cosmetic composition, cosmetic composition.
The method of claim 1,
The cosmetic composition is for antioxidant, cosmetic composition.
The method of claim 9,
The cosmetic composition is a cosmetic composition for inhibiting the oxidation of DPPH (1,1-diphenyl-2-picryl hydrazyl).
The method of claim 1,
The cosmetic composition is a cosmetic composition for skin cell regeneration.
The method of claim 12,
The cosmetic composition inhibits the expression of collagenase (collagenase), cosmetic composition.
The method of claim 1,
The cosmetic composition is a cosmetic composition for whitening.
The method of claim 14,
The cosmetic composition is a cosmetic composition that inhibits the production of melanin.
The method of claim 1,
The cosmetic composition is a cosmetic composition for moisturizing.
The method of claim 16,
The cosmetic composition is to enhance the skin barrier function or induce differentiation of keratinocytes, cosmetic composition.
delete

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