KR100903654B1 - Cosmetic compositions comprising Prunella Vulgaris extract and Adenosine - Google Patents

Abstract

The present invention relates to a cosmetic composition containing the extract of Hagocho and adenosine as an active ingredient, and more specifically, the extract of Hagocho promotes the proliferation of skin cells, inhibits the synthesis and synthesis of collagen of fibroblasts, and has an excellent antioxidant effect. It improves the skin wrinkles, and also provides a cosmetic composition that can effectively prevent skin aging by adenosine to promote the synthesis of DNA and protein in the dermal layer to exert a synergistic effect to improve skin wrinkles and skin elasticity.

Higocho extract, adenosine, skin aging, wrinkle improvement, elasticity improvement, cosmetics

Description

Cosmetic composition containing Prunella vulgaris extract and adenosine {Cosmetic compositions comprising Prunella Vulgaris extract and Adenosine}

The present invention relates to a cosmetic composition containing a hydrangea extract and adenosine, and more particularly, haegocho extract promotes the proliferation of skin cells, inhibits the synthesis and synthesis of collagen of fibroblasts, and has an excellent antioxidant effect for skin wrinkles. The present invention relates to a cosmetic composition which can effectively prevent skin aging by effectively improving the skin and adenosine to promote DNA and protein synthesis in the dermal layer to exert a synergistic effect on improving skin wrinkles and improving skin elasticity.

The skin is the largest organ of the human body, occupying about 16% of the total body volume, and an important protective barrier that protects the body from many deadly harmful factors, such as temperature, humidity, and ultraviolet radiation, which are in direct contact with the external environment and are likely to enter the body. Play a role. However, as they age, skin cells are damaged by various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111: 31-37 (1996)). Endogenous aging is a aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. Clinically, the elasticity is reduced, the skin texture is rough, deep wrinkles are formed, and pigments are deposited. Dermatol., 130: 87-95 (1994). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a marked decrease in elasticity.

Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, exposure of the skin to harmful free radicals or free radicals can lead to the formation of lipid peroxides through the oxidation of the body and to the transformation of the various proteins that make up the skin. 70-80%) collagen and elastic elastin proteins are the major proteins produced by fibroblasts and are involved in the skin's mechanical firmness, tissue cohesion and elasticity in the dermal layer (Journal of the american academy of dermatology, 21: 610-613 (1989). Collagen decreases with age and with increasing exposure to ultraviolet light.

Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen types I and III make up the constituents of the cytoplasm of the dermal layer, and collagen type IV is the major component of DEJ (Dermal Epidermal Junction). Collagen is located between the epidermal and dermal layers of the skin to regulate the permeation and transport of substances between the two layers, to control the differentiation of adjacent skin cells and to maintain the firmness of the skin. Collagen and elastin, the major structural components of the skin, are either denatured or reduced in biosynthesis by natural or external stimuli, thereby accelerating skin aging. In addition, the expression and activity of collagenase is also a major factor in accelerating skin aging.

Collagen synthesis promoters include retinoic acid, epidermal growth factor (EGF), TGF (trans-forming growth factor) and carcinogenic factors Cardinale G. et al., Adv.Enzymol., 41, p. 425, 1974). As a collagen synthesis promoting substance, Japanese Patent Application Laid-Open No. 08-231370 [Document 1] reports that an animal placental-derived protein is effective. Japanese Patent Application Laid-Open No. 08-208424 [Document 2] includes betulinic acid. It is reported to be effective. In addition, Japanese Patent Application Laid-Open Nos. Hei 09-40523 [Patent 3] and Hei 10-36283 [Patent 4] suggest that chlorella extract has an excellent fibroblast proliferation promoting effect as well as promoting collagen synthesis. In particular, epidermal growth factor acts as a potent promoter of epithelial cell, endothelial cell and fibroblast cell proliferation, and promotes the movement and proliferation of epithelial cells during epithelial cell defects. In addition, US Pat. Nos. 4,603,146 [Patent 5] and 4,877,805 [Patent 6], etc. specify that retinol is very effective in synthesizing collagen.

However, retinol is a substance that promotes collagen synthesis, but it is unstable and there is a limit to the amount of usage due to stability problems such as irritation and redness when applying the skin. Can't expect

On the other hand, the skin is exposed to ultraviolet light, and photochemical reactions that produce reactive oxygen species continue to occur, and these reactive oxygen species lead to damage to skin cells and tissues. They break the antioxidant defenses, which consist of antioxidant and non-enzymatic antioxidants, shifting the oxidant / antioxidant balance towards the oxidative state. Therefore, for the protection of a healthy body, a system that can effectively protect the generated free radicals must be included in the prescription of cosmetics. In other words, it is necessary to build an antioxidant defense system for skin that maintains an oxidant / antioxidant balance.

Materials known to have antioxidant activity so far include ascorbic acid, DL-alpha-tocopherol, beta-carotene, dibutylhydroxytoluene (BHT), and the like. However, since these materials are used as synthetic products, they may cause skin safety problems in long-term use or in large amounts, and thus, there is an urgent need for the development of new antioxidant cosmetics that can be applied to the skin more safely and usefully.

[Document 1] Japanese Patent Application Laid-Open No. 08-231370

[Patent 2] Japanese Patent Application Laid-Open No. 08-208424

[Patent 3] Japanese Patent Application Laid-Open No. 09-40523

[Patent 4] Japanese Patent Application Laid-Open No. 10-36283

[Reference 5] US Patent No. 4,603,146

[Document 6] US Patent No. 4,877,805

The present invention is to solve the problems of the prior art as described above, by containing the haegocho extract and adenosine as an active ingredient as an herbal extract that can be applied stably without irritation to the skin, the haegocho extract is Promotes the proliferation of skin cells, inhibits collagen synthesis and breakdown of fibroblasts, has an excellent antioxidant effect to improve skin wrinkles, the adenosine improves stability and persistence during skin penetration and promotes DNA and protein synthesis in the dermis The purpose of the present invention is to provide a cosmetic composition that can effectively prevent skin aging by exerting a synergistic effect on improving skin wrinkles and improving skin elasticity.

In order to achieve the above object, the present invention provides a cosmetic composition containing the hachocho extract and adenosine as an active ingredient.

At this time, the haejucho extract has the action of 증식) skin cell growth promoting action, ii) collagen synthesis promoting action, iii) collagenase inhibitory action, iii) antioxidant action, or iii) the combination of iii) to iii) The adenosine is contained as an active ingredient for improving the stability and sustainability during skin penetration, and for promoting DNA and protein synthesis in the dermal layer.

The cosmetic composition according to the present invention preferably contains 0.001 to 20.0% by weight of edible extract and 0.001 to 5.0% by weight of adenosine based on the total weight of the composition, and the edible extract may be hydrothermally extracted.

According to the present invention, the hyacinth extract promotes proliferation of skin cells, inhibits collagen synthesis promotion and degradation of fibroblasts, and has an excellent antioxidant effect to improve skin wrinkles. In addition, the adenosine improves stability and persistence during skin penetration and promotes DNA and protein synthesis in the dermis to exert a synergistic effect on improving skin wrinkles and improving skin elasticity, thereby effectively preventing skin aging.

In addition, the cosmetic composition according to the present invention is not irritating and stable to the skin and may be usefully used in the formulation of lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

Hereinafter, the present invention will be described in more detail.

The present inventors conducted research to search for anti-aging efficacy substances in natural native plants and animals, and as a result, it was confirmed that the extract of Hagocho was excellent in promoting the proliferation of skin cells, promoting collagen synthesis and inhibiting degradation, and having an excellent antioxidant effect. The present invention has been completed. In addition, since adenosine is an intracellular component, it was found to have excellent stability and sustainability when penetrating the skin, and to promote the synthesis of DNA and protein in the dermal layer, thereby helping to improve wrinkles, thereby exerting a synergistic effect on skin improvement.

Prunella vulgaris (Prunella vulgaris) is a perennial herbaceous plant called Lamiaceae and blooms from May to June and withers in summer and is called 'Gochocho'. The height is 30-40 cm, the leaves are long egg-shaped or long oval, opposite each other, short hairs, the stems are square, the tip is sharp, and the teeth are not present or not. The upper part of the stem forms a flower ear about 6 cm long, and small multicolored flowers are arranged in a columnar shape with tiles stacked up. After flowering, the stems of the upper outpost with the flowers are dried with bare leaves and used as a medicinal herb. The components of the vinegar include Tritrepenoid saponin, Oleanolic acid, Ursolic acid, Rutin, Hyproside, Cis-caffeic acid, and trans-caffeic acid. Trans-caffeic acid, Vitamin B, Vitamin C, Vitamin K, Carotene, Resin, Amaroid, Tannin, Essential Oil, Alkaloid ), Chlorogenic acid, water-soluble salts (about 3.5%, 68% of which is potassium chloride), etc., and the presence of rosmarinic acid is also found. 1988, Research on Hagocho Ingredients Lee, Jak-Pyung and 3 others, Pediatric Medical School Journal 1991, Chinese Medicine Dictionary]. Pleurotus has been used to treat chronic boils, cramps, acute mastitis, and lymph node tuberculosis in Chinese medicine. It has been used to destroy gongga (soak in the lower abdomen or bloated blood) and to remove each one and to treat numbness and numbness.

According to the present invention, the Hachocho extract as described above is excellent in promoting skin cell proliferation, collagen synthesis promoting action, collagenase inhibitory action, and antioxidant action to improve skin wrinkles and enhance skin elasticity.

In addition, adenosine is one of the elements constituting DNA, which is a gene, a compound of a base called adenine and a sugar called ribose. Adenosine is adenosine 5'-triphosphate (ATP), a major source of energy in cells when three phosphates are attached to sugars. Accordingly, adenosine is known as an important factor that plays a crucial role in all metabolism proceed smoothly while making up the body. According to the present invention, since adenosine is an intracellular component, it has excellent stability and sustainability when penetrating the skin, and synergistically works to improve skin wrinkles by promoting DNA and protein synthesis in the dermis.

The cosmetic composition according to the present invention contains the hachocho extract and adenosine as an active ingredient as described above.

The Hagocho extract can be prepared using various extraction methods known in the art, for example, can be prepared as follows.

Wash the vinegar with water and dry it in the shade. Dry vinegar is placed in an extraction container and extracted for a certain period of time at an appropriate temperature in an appropriate amount of extraction solvent. At this time, the extract may be filtered and concentrated using a vacuum rotary evaporator.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. Preferably, hot water extraction is performed by adding ethanol. The amount of the extraction solvent is preferably 2 to 10 times the dry weight of hachocho, the extraction method may be an extraction method such as bath extraction, reflux cooling extraction, ultrasonic extraction, etc. May be extracted five times. The deposition temperature at the time of extraction can be 30-70 degreeC, More preferably, it is 40-60 degreeC, Most preferably, maintaining about 50 degreeC is the most effective in the prevention of decay, discoloration, and odor. The deposition time is 5 to 20 hours, preferably 10 hours. Thus, after obtaining a vinegar extract, solid content is removed using a filter paper etc., a suspension is centrifuged and supernatant can be filtered under reduced pressure. And the filtered extract may be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

In the present invention, the haegocho extract is included in the present invention as long as it exhibits substantially the same effect using not only the extraction solvents listed above but other extraction solvents, and the haegocho extract is not only an extract by the extraction solvent, but also conventional It also includes purified extracts. Specifically, the haejucho extract used in the present invention is used as it is, or extracted by an extraction solvent, or purified after the extraction process, for example, membrane separation, such as ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography Purification procedures such as separation by (made for separation according to size, charge, hydrophobicity or affinity) can be used in addition. In addition, the haegocho extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying (or spray drying), and then may be contained in the composition as it is, or diluted in a solvent.

In addition, the cosmetic composition of the present invention preferably comprises 0.001 to 20.0% by weight of the edible extract of the total weight of the total cosmetic composition. At this time, when the edible extract contains less than 0.001% by weight, the skin cell proliferation promoting effect, collagen synthesis promoting effect, collagenase inhibitory effect and antioxidant effect are insignificant, and the content exceeds 20.0% by weight. If included, it may not be economical due to less synergy from excess input. Do. More preferably, the edible extract of 0.01 to 10.0% by weight, still more preferably 0.1 to 5.0% by weight. In addition, the adenosine is preferably included in 0.001 to 5.0% by weight based on the total cosmetic composition. At this time, if the content of adenosine is less than 0.001% by weight, the stability and sustainability of skin penetration due to the adenosine content, and the effect of promoting DNA and protein synthesis in the dermal layer are insignificant. This may not be possible.

The cosmetic composition according to the present invention includes components conventionally used in cosmetic compositions in addition to the Hagocho extract and adenosine, and may include, for example, conventional inclusion agents such as stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. Can be.

In addition, the cosmetic composition according to the present invention may be prepared in any formulation commonly prepared in the art, for example, containing solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants. It may be formulated by cleansing, etc., but is not limited thereto.

At this time, when the formulation of the cosmetic composition according to the present invention is a paste, cream or gel as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc And one or more selected from the group consisting of zinc oxide and the like.

In addition, when the formulation of the cosmetic composition according to the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo. One or more selected from the group consisting of eightes, propylene glycol, 1,3-butylene glycol, oils, glycerol aliphatic esters, polyethylene glycols, fatty acid esters of sorbitan, and the like can be used.

In addition, when the formulation of the cosmetic composition according to the present invention is a powder or spray, one or more selected from the group consisting of lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. , In the case of sprays in particular, may further comprise propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

In addition, when the formulation of the cosmetic composition according to the present invention is a suspension, a liquid diluent such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbent as carrier components Suspending agents such as non-elastic esters, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In addition, in the case where the formulation of the cosmetic composition according to the present invention is a surfactant-containing cleansing, as a carrier component, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate One or more selected from the group consisting of sarcosinates, fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives and ethoxylated glycerol fatty acid esters, and the like. have.

The cosmetic composition according to the present invention may specifically have a formulation of a lotion, an essence, a lotion, a cream, a pack, a gel, an ointment, a patch, or a spray agent, and is not particularly limited in the formulation, and the use or purpose of use of the cosmetic It can be produced in various ways.

The cosmetic composition according to the present invention effectively improves skin wrinkles and elasticity by synergistic effect of the extract of apricot and adenosine. This is specified in the examples below.

Hereinafter, the Example of this invention is illustrated. The following examples are provided to explain the present invention in more detail, and it will be apparent to those skilled in the art that the technical scope of the present invention is not limited by these examples. will be.

[Production Example 1]

<Preparation of Seaweed Extract>

50 g of algae was crushed finely using a crusher, immersed in 200 ml of 100% ethanol, and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Next, ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Experimental Example 1

Skin cell proliferation effect experiment

In order to examine the effect of Prunella vulgaris extract on skin cell proliferation, human normal fibroblasts (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate, and DMEM Incubated at 37 ° C. for 24 hours in medium (Sigma). Subsequently, the experimental group replaced with serum-free DMEM medium containing 50-500 μg / ml of the edible extract and the control group replaced with DMEM medium without serum containing no edible extract were further cultured for 24 hours. . Then, 10 μl each of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added thereto, followed by incubation for 4 hours. The medium was removed, shaken for 20 minutes by adding 100 μl of DMSO solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in the following [Table 1].

Higocho extract (µg / mL) Cell proliferation effect (%) Control - 50 38.2 100 51.9 200 70.5 300 75.8 500 83.7

As shown in [Table 1], compared to the control group not treated with the edible extract, the experimental group treated with the edible extract showed a very high fibroblast proliferation effect.

Experimental Example 2

<Collagen Synthesis Enhancement Experiment>

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the experimental group replaced with serum-free DMEM medium containing 50 ~ 500 µg / ml of the prepared Hagocho extract and the control group replaced with DMEM medium without serum containing the extract were further incubated for 48 hours. . After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a kit (Takara, Japan). The measured amount was converted to ng / 2x10 ⁴ cells, the results are shown in the following [Table 2].

Higocho extract (µg / mL) Collagen Synthesis Amount (ng / 2x10 ⁴ ) Control 102 50 110 100 124 200 148 300 162 500 190

As shown in [Table 2], it was found that the amount of collagen synthesized increased as the concentration of the hydrangea extract increased. This Hagocho extract was found to exhibit an excellent collagen synthesis promoting effect.

Experimental Example 3

<Collagenase Inhibitory Effect Experiment>

The collagenase production inhibitory activity of the haechocho extract was measured as follows.

The test was performed by placing human fibroblasts into 2 × 10 ⁴ cells / well in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum. Incubate until 70-80% growth. And after processing for 24 hours according to the concentration of Hachocho extract, the cell culture was collected. The cultured cell culture solution was measured by using a commercially available collagenase measuring instrument (Amersham Pharmacia Co., Catalog #: RPN 2610). First, the cultured cells were placed in a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours. After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, the coloring stimulant was added, causing color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the reaction (color development). The color of the reaction solution was yellow and the degree of yellow color was different according to the progress of the reaction. The absorbance of the yellowish 96-well plate was measured at 405 nm using an absorbance meter and the degree of synthesis of collagenase was calculated. At this time, the reaction absorbance of the prepared cell culture medium of the group not treated with the composition was used as a control. The results are shown in the following [Table 3]. In the results of the following [Table 3], the expression inhibitory ability is prepared by comparing the synthetic ability of the untreated group (control) to 100.

Higocho extract (µg / mL) Collagenase Expression (%) Control 100 50 94 100 88 200 80 300 70 500 65

As shown in [Table 3], it was found that as the concentration of the Prunella vulgaris extract was increased, it effectively inhibited the expression of collagenase in the cells.

Experimental Example 4

<Antioxidant Effect Test>

In order to examine the antioxidant effect of the Hachocho extract, Free Radical Scavenging Activity Test was conducted. The free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), and the stable free radical DPPH (1,1-diphenyl-2- picryhydrazyl (Sigma) reagent was used. 150 μl of the extract was added to 150 μl of a 2mM DPPH solution (ethanol in the case of Blanc), and 150 μl of these extracts were mixed and mixed, and the absorbance was measured at 517 nm for 30 minutes at room temperature. As a control for this, it was set as the case using purified water. After absorbance measurement for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below and the results are shown in the following [Table 4].

Higocho extract (µg / mL) Free radical scavenging effect (%) 50 10.5 100 18.3 200 30.4 300 40.2 500 69.2

As can be seen from the results shown in [Table 4], Hachocho extract was confirmed that the free radical scavenging ability is very excellent.

EXAMPLE

According to the present invention, in order to conduct a clinical test on the skin wrinkle and elasticity improvement effect of the cosmetic containing the Hachocho extract and adenosine, a cosmetic composition was prepared with the ingredients and contents shown in the following [Table 5]. At this time, the vinegar extract of Preparation Example 1 was contained 1.0% by weight, adenosine 0.04% by weight.

[Comparative Examples 1 and 2]

A cosmetic composition was prepared in the same manner as in the above example, but containing only the vinegar extract (Comparative Example 1) and not containing both (Comparative Example 2) as the present comparative example. Specific components and contents of the Comparative Examples are as shown in Table 5 below.

ingredient Content (% by weight) Example Comparative Example 1 Comparative Example 2 Vaseline 7.0 7.0 7.0 Liquid paraffin 5.0 5.0 5.0 Beeswax 2.0 2.0 2.0 Polysorbate-60 2.0 2.0 2.0 Sorbitan sesquioleate 2.5 2.5 2.5 Squalane 3.0 3.0 3.0 Propylene glycol 6.0 6.0 6.0 glycerin 4.0 4.0 4.0 Triethanolamine 0.5 0.5 0.5 Carboxy Vinyl Polymer 0.5 0.1 0.1 Tocopherol Acetate 0.1 0.1 0.1 Purified water To 100 To 100 To 100 Hagocho extract 1.0 1.0 - Adenosine 0.04 - - Incense, preservative a very small amount a very small amount a very small amount

Experimental Example 5

<Skin wrinkle improvement effect experiment>

In order to determine the skin wrinkle improvement effect of the cosmetic composition according to the Examples and Comparative Examples 1 and 2 were measured as follows in 30 healthy women aged 35 to 50 years.

First, divided into three groups, Example A for group A (10 people), Comparative Example 1 for group B (10 people), and Comparative Example 2 for C group (10 people) twice a day on the face (morning and Evening) was applied for 3 months. After 3 months, the degree of wrinkle improvement was evaluated by subject's questionnaire and image analysis of wrinkles.

The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in the following [Table 6].

Image analysis of wrinkles was performed by taking a replica of the eye under the eye before the experiment was started, and replicas immediately after the experiment was completed at the same area under the eye. Was measured. The measurement result of wrinkle density by image analysis was a reduction rate for wrinkle density before use, and the results are shown in the following [Table 7].

sample No improvement (persons) Minor improvements (persons) Moderate improvement (persons) Significant improvement Example One One 4 4 Comparative Example 1 One 3 4 2 Comparative Example 2 7 3 - -

sample Wrinkle Density Reduction (%) Example 45 Comparative Example 1 35 Comparative Example 2 10

As shown in the results of [Table 6] and [Table 7], in the case of the example containing the haegocho extract and adenosine at the same time, it was found that the skin wrinkle improvement effect is very excellent than Comparative Examples 1 and 2.

Experimental Example 6

<Skin elasticity effect experiment>

In order to determine the skin elasticity enhancing effect of the cosmetic composition according to the Examples and Comparative Examples 1, 2 were measured as follows.

First, we divided 30 healthy women (average age 37 years) over 20 years old into three groups, Example for group A (10 people), Comparative Example 1 for group B (10 people), and group C (10 people). The formulation of Comparative Example 2 was applied twice a day (morning and evening) for 12 weeks at a temperature of 24-26 ° C. and a humidity of 75% around the eyes, and then a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, The skin elasticity was measured using the Federal Republic of Germany. The test results were calculated with the value of R8 (R8 (12 weeks) -R8 (0 weeks)) of the cutometer MPA580, and the results are shown in the following [Table 8]. At this time, R8 value represents the nature of the skin viscoelasticity (Vicoelasticity).

sample Skin elasticity effect Example 0.67 Comparative Example 1 0.55 Comparative Example 2 0.27

As shown in the results of [Table 8], in the case of the example containing the haegocho extract and adenosine at the same time, it was confirmed that the skin elasticity enhancing effect is significantly increased than Comparative Examples 1 and 2.

Experimental Example 7

<Stability Test of Formulation>

In order to test the stability of the cosmetic composition containing the Hagocho extract and adenosine was carried out in the following manner.

The cosmetic composition according to Examples and Comparative Examples 1 and 2 was stored in an opaque glass container in a constant temperature bath maintained at 45 ° C. for 12 weeks, and the opaque candle was stored in a completely shaded refrigerator kept at 4 ° C. After storing for 12 weeks in a container, the discoloration, the smell, the degree of separation was measured. The results are shown as averages in the following [Table 9]. At this time, the product discoloration, odor, separation degree was evaluated by classifying into the following six grades.

0: no change

1: very little separation (discoloration)

2: A little separation (discoloration)

3: Slightly separated (discolored)

4: severely separated (discolored)

5: extremely severe separation (discoloration)

 division Formulation Stability Example Comparative Example 1 Comparative Example 2 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0.5 0 0.4 0 0 0 Deodorant 0.3 0 0.2 0 0 0 detach 0 0 0 0 0 0

As can be seen from the above [Table 9], the Examples and Comparative Examples were stable at 45 ° C. with little discoloration, discoloration and separation. This indicates that the Hagocho extract and adenosine are present in a stable state in the formulation.

As can be seen from the above results, according to the present invention it can be seen that the cosmetic composition containing the hachocho extract and adenosine at the same time has a very good skin wrinkle improvement and elasticity effect by synergistic action. In addition, it can be seen that the cosmetic composition has formulation stability.

Hereinafter, on the basis of the results of the above experimental example, a formulation example of the cosmetic composition containing the haechocho extract and adenosine of the present invention is presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1

<Softener (Skin Lotion)>

To the softener longevity (skin lotion) with the ingredients and contents shown in the following [Table 10] was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Hagocho extract 0.5 Adenosine 0.04 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2

<Nutrients (Milk Lotion)>

It was prepared according to the conventional method for the nutrient nutritional water (milk lotion) with the ingredients and contents shown in the following [Table 11].

Compounding ingredients Content (% by weight) Hagocho extract 1.0 Adenosine 0.04 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

[Formulation Example 3]

<Nutrition Cream>

Nutritional creams were prepared according to the conventional method with the ingredients and contents shown in the following [Table 12].

Compounding ingredients Content (% by weight) Hagocho extract 2.0 Adenosine 0.04 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

[Formulation Example 4]

<Massage cream>

Massage cream was prepared according to the conventional method with the ingredients and contents shown in the following [Table 13].

Compounding ingredients Content (% by weight) Hagocho extract 0.5 Adenosine 0.04 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5

<Pack>

The pack was prepared according to a conventional method with the ingredients and contents shown in the following [Table 14].

Compounding ingredients Content (% by weight) Hagocho extract 1.0 Adenosine 0.04 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (6)

It contains Higocho extract and adenosine as active ingredients, The Hagocho extract is contained in an amount of 0.001 to 20.0% by weight based on the total weight of the cosmetic composition, the adenosine is contained in an amount of 0.001 to 5.0% by weight based on the total weight of the cosmetic composition, The hachocho extract and the adenosine are contained in a weight ratio of 25: 1 cosmetic composition, characterized in that to improve the skin wrinkles. delete delete delete delete The method of claim 1, The cosmetic composition is a cosmetic composition, characterized in that having a formulation of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.