KR101987421B1 - Cosmetic composition for improving antioxidation and skin wrinkle comprising bertholletia excelsa seed oil, plukenetia volubilis seed oil, mangifera indica extract and fragaria chiloensis extract - Google Patents

Abstract

The present invention relates to a process for the preparation of < RTI ID = Excelsa Seed Oil, African Walnut ( Plukenetia Volubilis Seed) Oil, Mangifera Indica (Mango) Fruit) Extract and Strawberry ( Fragaria Chiloensis (Strawberry) Fruit) extract as an active ingredient. The cosmetic composition may exhibit an antioxidant or anti-aging effect.

Description

FIELD OF THE INVENTION The present invention relates to a cosmetic composition for improving antioxidant and skin wrinkles, which comprises an extract of Brazil nuts seed, an African walnut oil, a mango extract, and a strawberry extract. The present invention relates to cosmetic compositions for improving skin wrinkles, CHILOENSIS EXTRACT}

The present invention relates to a process for the preparation of < RTI ID = Excelsa Seed Oil, African Walnut ( Plukenetia Volubilis Seed) Oil, Mangifera Indica (Mango) Fruit) Extract and Strawberry ( Fragaria Chiloensis (Strawberry) Fruit) extract as an active ingredient.

Skin is a membrane that covers the outside of the body. It plays a role of protecting and protecting internal organs and other body organs by performing various physiological functions to protect the body from environmental factors such as external stimuli, obstacles and drying do.

However, as we get older, the secretion of various hormones that regulate metabolism is reduced internally, and the function of immune cells and the activity of cells are lowered. Externally, ozone layer destruction increases the ultraviolet ray content in the sunlight. As the environmental pollution becomes more intense, the excessive physical and chemical stimuli and stress caused by the increase of free radicals and active harmful oxygen, And the skin is damaged by promoting the generation of spots and freckles due to melanin deposition and skin aging phenomenon.

In order to prevent skin aging and damage due to such internal and external factors, physiologically active substances obtained from various animals, plants, microorganisms and the like are added to cosmetics in order to maintain healthier and more beautiful skin, Efforts have been made to maintain skin health by promoting skin metabolism by activating cells, and thus, cosmetics and external preparations for skin have been developed.

However, these materials have limited use due to safety problems such as irritation, erythema, and redness when applied to the skin, or the effect is insignificant, and it is difficult to substantially improve the skin function. Therefore, there is a demand for development of a novel composition for external application for skin, which is safer and more effective for a living body than conventional skin external composition compositions.

Korean Patent Laid-Open No. 10-2012-015881 Korean Patent No. 10-0524291

It is an object of the present invention to provide a process for the preparation of bertholletia Excelsa Seed Oil, African Walnut ( Plukenetia Volubilis Seed) Oil, Mangifera Indica (Mango) Fruit) Extract and Strawberry ( Fragaria Chiloensis (Strawberry) Fruit) extract as an active ingredient.

In order to accomplish the above object, an aspect of the present invention is to provide a method of manufacturing a berry nut ( Bertholletia Excelsa Seed Oil, African Walnut ( Plukenetia Volubilis Seed) Oil, Mangifera Indica (Mango) Fruit) Extract and Strawberry ( Fragaria Chiloensis (Strawberry) Fruit) extract as an active ingredient.

In the present invention, " Bertholletia Excelsa Seed) "is a seed of a Brazilian nut tree. In the fruit of a Brazilian nut, the seeds are arranged like an orange. Brazil nuts are useful because they have a high oil content, and indigenous Brazilian tribes use the oil extracted from the nuts in the wounded area or as food. In addition, Brazilian nuts are rich in natural selenium, which increases immunity and helps prevent thyroid dysfunction.

In the present invention, " Plukenetia Volubilis Seed "is a name for a tree in the Lovoa family called Dibetou in Africa to distinguish it from Walnut in other countries in the UK. The oil obtained by squeezing the seeds of the African walnut is called various names such as Incan Inch Oil, Inca Oil, Saka Ink Oil and Quaternary Inch Oil. It is known that the nutritional component of the oil is composed of digestible form, so that it can be absorbed rapidly from the human body and does not inhibit absorption of other important nutrients.

In the present invention, " Mangifera Indica (Mango) Fruit "is a fruit belonging to the genus Lacquer, originating in Southeast Asia and mainly distributed in subtropical regions such as Myanmar, northern India, and Mexico. Mangos are rich in vitamin A, and carotene is known to be very similar to green vegetables. It has been reported that the stem skin, leaves and flesh part of mangoes as medicinal parts have antioxidant, anti-inflammatory, anti-allergic analgesic and immunomodulating effects.

In the present invention, " strawberry ( Fragaria Chiloensis (Strawberry) Fruit "is a perennial plant of Rosaceae Rosaceae originating in Central Europe. It is adaptive to temperature and grows from the coast near the equator to the area near the Arctic. Leaves come out of roots and petiole is long. The small leaf is 3 ~ 6㎝ long and 2 ~ 5㎝ wide and is obovate with a sawtooth edge. The main ingredients of the fruit are carbohydrates, calcium, phosphorus, and carotene. Vitamin C is a high content of vitamins to regulate the function of the adrenal cortex to regulate hormones, pectin is rich in the flow of blood is said to be smooth.

The " extract " of the present invention includes not only the mango extract and the strawberry extract but also an extract obtained by extracting a mixture of the above extracts, a mango and a berries gingival mixture, a dilute or concentrated liquid of the extracts, Extracts of all the formulations which can be formed using the extract itself and the extract, such as a preparation or a purified product of the extract, or a mixture thereof.

The extract of the present invention can be extracted from natural, hybrid, or mutant plants of the respective plants, and can also be extracted from plant tissue cultures.

The method for extracting the extract of the present invention is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

The kind of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, anhydrous or lower alcohol of C ₁ to C ₄ , a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, chloroform Or a mixture of two or more of them.

More specifically, water (or distilled water) may be used as an extraction solvent in the present invention. The mangoes and / or strawberries may be extracted at least once using the solvent to prepare a solvent extract. The dried extract obtained by vacuum distillation of the solvent extract, followed by lyophilization or spray drying may be prepared. In one specific example of the present invention, a mango extract and a strawberry extract were prepared using water (or distilled water), and a mixture thereof was prepared by mixing them with Brazil nut seed oil and African walnut oil (Example 1).

In the present invention, the extract obtained by heat extraction or cold extraction is filtered to remove suspended solid particles by filtration, for example, nylon or the like, or filtered using a freezing filtration method or the like, Spray drying and the like.

Specifically, the Brazil nut seed oil, the African walnut oil, the mango extract, and the strawberry extract may be contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10.

In one embodiment of the present invention, a mixture prepared by mixing the Brazil nut seed oil, the African walnut oil, the mango extract and the strawberry extract at a weight ratio of 5: 5: 5: 5 was prepared. . In particular, all the raw materials of the present invention are natural-derived substances, so that they are not toxic and have a grade of 1 on the EWG (Environmental Working Group) scale. Therefore, even if a specific extract is contained in excess, there is no adverse effect.

Specifically, the cosmetic composition comprising the present invention of Brazil nut seed oil, African walnut oil, mango extract, and strawberry extract as an active ingredient may be antioxidant or anti-aging.

In the present invention, " antioxidant " means an oxidation-inhibiting action. Although the human body has a balance of an oxidation-promoting substance and an oxidation-inhibiting substance, due to various factors, , Oxidative stress is induced in the living body, leading to cell damage and pathological diseases. Reactive oxygen species (ROS), which is a direct cause of oxidative stress, are chemically unstable and highly reactive, and can easily react with various biomaterials such as DNA, protein, lipid and carbohydrate. Attacks, causing irreversible damage to cells and tissues, or causing mutations, cytotoxicity and cancer, and may also be a direct cause of aging. By removing or reducing these active oxygen species, antioxidant effects can be obtained, thereby preventing aging and maintaining health.

In one embodiment of the present invention, free radical scavenging activity was measured to confirm antioxidative effect. When a mixture containing both Brazil nut seed oil, African walnut oil, mango extract and strawberry extract was applied, (Table 1). ≪ tb > < TABLE >

In the present invention, " anti-aging " refers to inhibiting aging or against aging. As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which constitute the skin, are changed or decreased, and oxidative stress due to free radicals and active noxious oxygen is experienced. In addition, as the aging progresses, changes such as reduction of wrinkles of the skin and reduction of elasticity appear.

In one embodiment of the present invention, the antioxidative effect, the increase in collagen production and the wrinkle-reducing effect of the composition of the present invention were confirmed. Specifically, excellent antioxidative effects were confirmed when a mixture containing both Brazil nut seed oil, African walnut oil, mango extract, and strawberry extract was applied (Table 1), and the composition promoted collagen synthesis, thereby increasing the total amount of collagen (Table 2).

Collagen is a major substrate protein produced in the fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical durability of the skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation It is well known. Furthermore, it is known to be closely related to skin wrinkle formation.

In one embodiment of the present invention, it was confirmed that the composition of the present invention is excellent in promoting collagen synthesis, and furthermore, when a cosmetic composition containing the composition as an active ingredient was applied, it was confirmed that skin wrinkles were actually improved )

These results indicate that the composition of the present invention ultimately exhibits excellent skin aging-preventing effect and can be widely used for anti-aging purposes.

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening lotion, a pack, a soft water, a latex, a makeup base, But are not limited to, those selected from the group consisting of solutions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powdered foundations, emulsion foundations, wax foundations, patches and sprays no.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the above cosmetic composition, Brazil nut seed oil, African walnut oil, mango extract, and strawberry extract may be included in an amount of 0.001% by weight to 30.0% by weight based on the total weight of the cosmetic composition. More specifically from 0.005% to 20% by weight. There is an advantage that excellent antioxidant or anti-aging effect is exhibited within the above range, and there is an advantage that the formulation of the composition is stabilized.

The cosmetic composition comprising the Brazil nut seed oil, the African walnut oil, the mango extract and the strawberry extract of the present invention as an active ingredient is excellent in antioxidant or anti-aging effect.

In addition, since the composition of the present invention is a substance derived from a natural substance, it has no toxicity and does not cause side effects even when it is applied to human body, and thus it is highly utilized as a cosmetic composition. Particularly, all the raw materials used in the present invention are classified as EWG (Environmental Working Group) grade 1, and thus it has been proved that the raw materials are excellent in safety.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example  One. Brazil nuts  oil, African Walnut  Preparation of a mixture of oil, mango extract and strawberry extract

Mangos and strawberries were weighed into a flask and then extracted with 1,000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an extract of each of mango and strawberry. Each of the above extracts was mixed with EWG 1 grade of Brazil nut seed oil and EWG 1 grade of African walnut oil at a weight ratio of 5: 5: 5: 5.

Comparative Example  One. Brazil nuts  oil

EWG 1 grade Brazil nut seed oil was used.

Comparative Example  2. African Walnut  oil

EWG grade 1 African walnut oil was used.

Comparative Example  3. Preparation of mango extract

Mango dry weight of 100 g was placed in a flask and extracted with 1,000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mango extract.

Comparative Example  4. Preparation of strawberry extract

100 g of strawberry dried weight was put into a flask and extracted with 1,000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a strawberry extract.

Experimental Example  1. Identification of antioxidant effect

The antioxidative ability of Example 1 and Comparative Examples 1 to 4 was confirmed. DPPH (1,1-diphenyl1-2-picry1hydrazy1) was used to measure free radical scavenging activity. Specifically, 0.6 mL of each of Example 1 and Comparative Examples 1 to 4 was added to 2.4 mL of 4.0 x 10 ^-4 M DPPH solution, the mixture was shaken for 10 seconds with a vortex mixer, left at room temperature for 30 minutes, and absorbance was measured at 517 nm. And the antioxidant activity was decreased. At this time, ascorbic acid, which is an antioxidant, was used as a control group for determining the absorbance reduction ratio, and the same contents as in the samples were added, and the same measurements were carried out.

sample DPPH (%) Antioxidant activity comparison (%) Ascorbic acid 28 100 Example 1 87 316 Comparative Example 1 54 192 Comparative Example 2 61 218 Comparative Example 3 67 239 Comparative Example 4 58 202

As shown in Table 1, it can be seen that Example 1 and Comparative Examples 1 to 4 exhibited antioxidative effects. However, in Example 1, it was confirmed that the antioxidative effect was superior to that of Comparative Examples 1 to 4 there was.

Experimental Example  2. Confirmation of increased collagen production

Example 1 and Comparative Examples 1 to 4 were added to a culture solution of human fibroblasts to examine the effect of promoting collagen synthesis at the cellular level to increase the total amount of collagen. The total amount of collagen was measured using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit). The fibroblasts were cultured in different concentrations (% by weight) in Example 1 and Comparative Examples 1 to 4 (fibroblasts were cultured and subjected to MTT test ([Reference: Mossman T., The results are summarized as follows: 1) In the absence of cytotoxicity, a median of 0.5% of the concentrations below 1% was used. And the degree of increase in the total amount of collagen was evaluated.

Each sample was added to the culture medium of human fibroblasts and cultured for 1 day. Then, the culture solution was taken and the degree of increase in the total amount of collagen at each concentration was measured at 450 nm using a PICP EIA kit using a spectrophotometer. For the comparison of the effects, the extent of increase in the total amount of collagen was measured in the same manner for the culture medium (negative control) of fibroblasts to which nothing had been added and the sample (positive control) added with vitamin C to a final concentration of 52.8 / / ml . The total amount of collagen was measured as the UV absorbance, and the rate of increase of the total amount of collagen was calculated by the ratio of the total amount of collagen relative to the control, and the results are summarized in Table 2 below. The experiment was repeated three times.

sample Total collagen (Absorbance) Growth rate (%) Negative control group 452 - Vitamin C (52.8 / / ml)
(Positive control group) 533 117.92 Example 1 566 125.22 Comparative Example 1 521 115.26 Comparative Example 2 518 114.60 Comparative Example 3 535 118.36 Comparative Example 4 503 111.28

As shown in Table 2, it was confirmed that the effect of increasing the total amount of collagen by promoting collagen synthesis was superior to that of Comparative Examples 1 to 4 in Example 1. In particular, in each of Comparative Examples 1 to 4, collagen synthesis effect similar to or less than that of vitamin C, which is a known wrinkle improving substance, was shown, whereas in Example 1 including both of them, collagen synthesis effect was remarkably increased .

Experimental Example  3. Confirm wrinkle improvement effect

Fifty female subjects aged 19 to 62 were divided into five groups, and the composition according to the following Table 5 was applied evenly on the face after morning and evening cleansing for 4 weeks and fully absorbed in the face. ANTERA 3D (Miravex, Ireland) was applied for evaluation of wrinkle improvement. Instrument measurement was performed before the test substance and after 4 weeks of use.

The same test person measured the left part of the left eye of each subject and the same area was measured by overlapping with the image measured before the test material for the reproducibility of the measurement. The captured images were matched using ANTERA pro software, which is dedicated to ANTERA 3D, and the matched measurement sites were used for analysis. The measured values were analyzed using the Indentation index, which is a measurement variable.

Raw material name Content (% by weight) Cetearyl alcohol 2 2 2 2 2 Cetearyl glucoside One One One One One Jojoba seed oil 5 5 5 5 5 Silica 0.5 0.5 0.5 0.5 0.5 glycerin 10 10 10 10 10 Example 1 5 - - - - Comparative Example 1 - 5 - - Comparative Example 2 - - 5 - - Comparative Example 3 - - - 5 - Comparative Example 4 - - - - 5 Purified water To 100 To 100 To 100 To 100 To 100

Table 4 shows the change of the Wrinkles small value after 4 weeks of using the essence type composition as shown in Table 3 above. Wrinkles As the variation of small value increases, the effect of improving wrinkles is excellent.

division Wrinkles small value Example 1 1.36 Comparative Example 1 1.26 Comparative Example 2 1.23 Comparative Example 3 1.21 Comparative Example 4 1.19 Positive control group 1.22 Negative control group 0.47

As shown in Table 4, it was confirmed that the wrinkle-reducing effect of Example 1 was superior to that of Comparative Examples 1 to 4. In particular, in each of Comparative Examples 1 to 4, the wrinkle-improving effect was similar to that of the positive control group, whereas the wrinkle-improving effect was remarkably increased in Example 1 including all of them.

Manufacturing example 1. Bonn  Preparation of an essence containing a mixture of the invention

The cosmetic composition of the essence formulation was prepared as shown in the following Table 5 (unit: wt%).

Raw material name Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Invention mixture 5 Purified water To 100

Manufacturing example 2. Bonn  Cream manufacture containing a mixture of the invention

A cream form cosmetic composition was prepared as shown in Table 6 below (unit: wt%).

Composition Content (% by weight) Invention mixture 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Suitable amount incense Suitable amount Purified water to 100

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (5)

An extract of Bertholletia Excelsa Seed oil, Plukenetia Volubilis Seed oil, Mangifera Indica (Mango) Fruit extract and Fragaria Chiloensis (Strawberry) Fruit extract,
The extract is obtained by cold extraction of distilled water as a solvent,
Wherein the weight ratio of the Brazil nut seed oil, the African walnut oil, the mango extract, and the strawberry extract is in a weight ratio of 5: 5: 5: 5. delete delete The method according to claim 1,
Wherein the Brazil nut seed oil, the African walnut oil, the mango extract and the strawberry extract are contained in an amount of 0.001% by weight to 30.0% by weight based on the total weight of the cosmetic composition. delete