KR101110301B1 - Whitening cosmetics composition containing the sorbaria sorbifolia var. stellipila extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening comprising Sorbaria sorbifolia var. Stellipila extract.

According to the present invention, since the extract of Sorbaria sorbifolia var. Stellipila has melanin production inhibitory activity and skin whitening effect at the same time, the extract of shit tree is useful as various functional cosmetics.

Sorbaria sorbifolia var.stellipila, melanin production inhibitory skin whitening

Description

Whitening cosmetics composition containing the sorbaria sorbifolia var. stellipila extract}

The present invention relates to a cosmetic composition for skin whitening comprising Sorbaria sorbifolia var. Stellipila extract.

In the human body, the skin acts as a protective film to protect the living body from the external environment. It prevents the loss of biological components such as water or electrolytes and prevents harmful substances from entering the body. .

Human skin has various physicochemical changes during the aging process, and its causes are classified into internal aging and photo-aging. That is, it may be caused by ultraviolet rays, stress, disease state, environmental factors, wounds, activation of free radicals with age, and when this condition is intensified, it destroys the antioxidant defense network existing in the living body and destroys cells and tissues. Damage to promote adult disease and aging. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of protein is caused by the breakdown of collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are the skin's connective tissues, resulting in severe excessive inflammatory reactions and disruption of skin elasticity. This leads to a situation of reduced immune function. Therefore, cell membranes are protected by eliminating free radicals generated by the body's metabolic processes, irradiated with ultraviolet radiation or inflammatory reactions.Also, damaged cells must be regenerated by active metabolism to proliferate the skin. You can recover quickly and maintain healthy skin.

In July 2001, the Korea Food and Drug Administration (KFDA) issued a list of functional cosmetic standards and test methods. The substances that help whitening are listed as the ingredient of the extract of mulberry and oil-soluble licorice extract. In addition to [General Information on Cosmetics Policy, Korea Food and Drug Administration, pp243-250, 2003], natural products such as green tea, baekbaekpi, betel nut, gold and wild ginseng are used as active ingredients of cosmetics. [Kim Young-Joong, Recent Trends in Natural Products Research, Journal of the Korean Society of Cosmetic Products, 29, pp1-15, 2003; Ahn, Deok-Kyun, Korean Theory of Skin Beauty, Journal of the Korean Cosmetic Society, 29, pp79-87, 2003] As such, many natural products have been studied as cosmetics, and various researches and productions of herbal cosmetics using herbal medicines used in oriental medicine It is becoming. Efforts have been made to find cosmetic ingredients with excellent stability among plant extracts such as herbal medicines, vegetables, fruits, and flowers that are used as herbal medicines.

The inventors of the present invention have completed the present invention by confirming that the bark tree extract has an excellent melanin production inhibitory activity and has a skin whitening effect. That is, the cosmetic composition for skin whitening comprising the bark tree extract is reported for the first time through the present invention.

An object of the present invention is to select an extract prepared from the natural product of the bark tree extract of various natural products, to confirm the efficacy as a raw material of the cosmetic, to develop a method of using the same, to prepare a cosmetic having a predetermined functionality by using this raw material To provide a way to do this.

That is, an object of the present invention is to provide a natural extract that is applicable to skin cosmetics and exhibits melanin production inhibitory activity and skin whitening effect when contained in cosmetics.

In order to solve the problem of the present invention, it is characterized by a cosmetic composition for skin whitening containing Sorbaria sorbifolia var. Stellipila extract.

Barberry extract has excellent melanin inhibitory activity and skin whitening effect.

Since the cosmetic composition of the present invention contains the bark tree extract, it has a useful effect as a cosmetic composition for skin whitening.

The cosmetic composition of the present invention is prepared in various formulations, such as lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) or water-in-oil (W / O) type emulsion, ointment, functional for skin whitening Has the effect of applying to cosmetics.

The present invention will be described in more detail as follows.

The cosmetic composition according to the present invention is characterized in that it contains chitin extract as the main active ingredient.

Natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural ingredients has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing. Thus, the present inventors have studied the possibility of applying to cosmetics in various natural products, select the bark tree and manufacture the extract from it, confirm through the skin whitening effect experiment, it can be expected that the efficacy as a cosmetic raw material Found out.

Sorbaria sorbifolia var.stellipila is a deciduous broad-leaved arborescent belonging to the family Rosaceae, and other names are gash bark, walnut tree, rice buckthorn, jinjimae, etc. It is called 東北 珍珠 梅, and it is called Sue Tak because of its flower-like shape. It grows in small mountain valleys and streams, and is one of the earliest flowering trees in the early spring. It is good for wheat plants because it has a lot of honey, and it is beautiful for blooming in the early summer. It is very good and eats new sprouting young sprouts in early spring. Stem, fruit ear, root, stem bark all can be used as medicine, mainly stem and percutaneous (莖 皮) is called medicinal (珍珠 梅) medicinal and collected in the autumn or winter to write in the sun. Ingredients include sorbifolin (scutellarein-7-o-xylorhamnoside) in leaves, kaempgerol-3-arabofuranoside in flowers, astragarin, quercetin-3-glucoin Ronide (quercetin-3-glucuronide), isorhamnetin-3-glucoside, scoutellarein, chlorogenic acid, arbutin, scutellalane-7- o-α-1-rhamnoside and flavonoid compounds are contained. Pharmacological action of the blood, (활), larvae, small sarcoma, jitong (止痛) has the efficacy of fractures, bruises, sprains, spontaneous arthritis, osteoporosis and bones are used to strengthen the congestion It is known to have the effect of relieving pain due to pain. The flowers of the bark tree have been used as medicine for flavor and insect repellent.

The bark tree extract used as a cosmetic raw material in the present invention is a leachate obtained by leaching from the bark tree outpost, or a concentrate obtained by partially or totally concentrating the leachate, or an extract extracted by drying the concentrate again. It contains the chemical substance itself which exhibits the main effect contained in the extract.

On the other hand, the content of the bark tree extract according to the present invention is preferably in the range of 0.001 to 30.0% by weight based on the lyophilized weight relative to the entire cosmetic composition. If the content of the extract is less than 0.001% by weight, there is almost no skin improvement effect, and when the content of the extract exceeds 30.0% by weight, it is not economical because the degree of increase of the effect on the content is insignificant.

In addition, when using the extract of the barberry tree according to the present invention as a cosmetic composition, it can be used in the form of lyophilized powder, as well as in the form of a liquid dispersed or dissolved in water or a conventional organic solvent. Even if included in the cosmetic composition in a liquid form can be used by adjusting the amount used within the range of 0.001 to 30.0% by weight on the basis of lyophilized weight.

In addition, the cosmetic composition according to the present invention, in addition to the above extract, other ingredients that can give a synergistic effect to the main effect, preferably within the range that does not impair the main effect for the purpose of the present invention, for example, green tea extract, mulberry extract It may contain natural products such as licorice extract, lettuce extract, betel nut extract, golden extract, and wild ginseng extract.

In addition, the bark tree extract according to the present invention is to extract the dried starch tree outpost using an appropriate extraction solvent. As the extraction solvent used in the present invention, a single solvent or two or more mixed solvents selected from purified water and a conventional organic solvent may be used. Specifically, purified water, alcohols having 1 to 6 carbon atoms, glycerin, alkylene glycols having 2 to 6 carbon atoms, alkanes having 1 to 6 carbon atoms, halogenated hydrocarbons having 1 to 6 carbon atoms, alkyl alkanoates having 2 to 6 carbon atoms, and mixtures thereof Extraction solvents selected from can be used. More specifically, an extraction solvent selected from purified water, methanol, ethanol, glycerin, ethylene glycol, butylene glycol, propylene glycol, dichloromethane, dichloroethane, hexane, ethyl acetate, and mixtures thereof may be used. After extracting the starch of the bark tree with the extraction solvent, and cold extraction and filtration, the filtrate is concentrated under reduced pressure at 50 ℃ or less and the freeze-dried concentrate under reduced pressure to obtain the bark tree extract according to the present invention in powder form. If necessary, the lyophilized barberry extract powder may be dispersed or dissolved in water or a conventional organic solvent to obtain a liquid form.

The cosmetic composition of the present invention is in the form of an aqueous, aqueous-alcoholic or oily solution, oil-in-water or oily water or multi-emulsion, aqueous or oily gel, liquid, paste or solid anhydrous product, oil dispersion in aqueous phase using globules. And more preferably in the form of lipid endoplasmic reticulum in ionic and / or nonionic form.

The cosmetic composition of the present invention may be somewhat fluid and may have the appearance of white or colored cream, ointment, milk lotion, serum, essence, paste or mousse. It may optionally be applied to the skin in the form of an aerosol or may be in the form of a solid, such as a stick. It may be used as a skin care product and / or makeup product.

In a known manner the compositions according to the invention can also be used as conventional auxiliaries in the cosmetic field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants. And dyes. The amounts of these various adjuvants are amounts commonly used in the art, such as in the range of 0.01 to 20% by weight relative to the total weight of the cosmetic composition. Depending on its nature, such adjuvants may be incorporated into the fatty phase, aqueous phase, lipid vesicles and / or nanoparticles. In any case, the adjuvant and its proportions will be chosen so as not to adversely affect the desirable properties of the cosmetic composition according to the invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative description of the present invention and the scope of the present invention is not limited to these Examples.

[Example]

Preparation Example 1 Preparation of Ethanol Aqueous Extract of Barberry

The dried bark tree was chopped and refluxed three times for 5 hours with 95% (V / V) ethanol solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, thereby obtaining a tree extract powder (yield 2.0% based on 6.0 g of trees).

In addition, a solution obtained by dissolving the above-obtained Syrup extract powder at a concentration of 3% by weight in a mixed solution of 7/3 volume ratio of purified water / butylene glycol was used as a sample solution in the following example.

Preparation Example 2 Preparation of Propylene Glycol Extract of Barberry

In the same manner as in Preparation Example 1, the bark tree extract powder (1.0% yield based on 3.0 g) was prepared, but propylene glycol was used in place of the 95% (V / V) ethanol aqueous solution as the extraction solvent.

Preparation Example 3 Preparation of Ethyl Acetate Extract of Barberry

In the same manner as in Preparation Example 1, the bark tree extract powder (yield 1.5% based on 4.5 g) was prepared, but ethyl acetate was used in place of the 95% (V / V) ethanol aqueous solution as the extraction solvent.

Preparation Example 4 Preparation of Water Extract of Barberry

In the same manner as in Preparation Example 1, the bark tree extract powder (a yield of 0.7% based on 2.1 g) was prepared, but water was used instead of the 95% (V / V) ethanol aqueous solution as the extraction solvent.

Preparation Example 5 Preparation of Dichloromethane Extract of Barberry

In the same manner as in Preparation Example 1, the bark tree extract powder (yield 0.57% based on 1.7 g) was prepared, but dichloromethane was used as a solvent instead of 95% (V / V) ethanol solution.

Preparation Example 6 Preparation of Hexane Extract of Barberry

In the same manner as in Preparation Example 1, the bark tree extract powder (yield 1.4% based on 4.2 g) was prepared, but hexane was used as a solvent instead of 95% (V / V) ethanol aqueous solution.

Example 1 Antioxidant Effect Using NBT Method

In order to measure the antioxidant effect of the bark tree extract obtained in Preparation Example 1, the antioxidant samples such as retinol and BHT were compared in a laboratory condition, and the antioxidant activity was measured by NBT method. In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase was measured by NBT method, and the effect of removing the active oxygen by the test substance, that is, the active oxygen scavenging effect, was evaluated. Free radicals are generated by xanthine and xanthine oxidase, and the active oxygen scavenging rate is measured by measuring the blue color produced by reaction of the reactive oxygen with Nitro Blue Tetrazolium (NBT) at a wavelength of 560 nm. It was.

The antioxidant activity measurement method by the NBT method will be described in more detail.

2.4 mL of 0.05 M sodium carbonate, 0.1 mL of 3 mM xanthine solution, 0.1 mL of 3 mM EDTA solution, 0.1 mL of BSA solution, and 0.1 mL of 0.72 mM NBT solution were added to a Bayer bottle, and the absorbance (S _o ) at 560 nm was measured. It was.

0.1 mL of each of the barberry extract, retinol and BHT obtained in Preparation Example 1 was added thereto as a sample solution, and the mixture was left at 25 ° C. for 10 minutes. Then, 0.1 mL of xanthine oxidase solution was added and stirred at high speed, followed by incubation for 20 minutes at 25 ° C. 0.1 mL of 6 mM copper (II) chloride solution was added to stop the reaction, and the absorbance (S _t ) at 560 nm was measured.

For the blank test solution, only distilled water was used instead of the sample solution. The rest of the experiment was performed in the same manner as described above, and then the absorbance at 560 nm (B _t ) was measured.

Merely a 0.1 mL distilled water in place of the blank (Blank) is a xanthine oxidase solution of the sample solution was used, and the remaining course of the experiment was measured and the absorbance (B _o) at 560 nm after performing in the same manner as described above.

Substituting the absorbance value measured in the above Equation 1 to calculate the antioxidant activity by the NBT method, the results are shown in Table 1 below.

In Equation 1, S _t represents the absorbance value measured after the enzymatic reaction of the sample solution, B _t represents the absorbance value measured after the enzymatic reaction of the blank test solution, and S _o is added to the sample solution. The absorbance value measured previously is shown, and B _o represents the absorbance value measured before adding the enzyme to the blank test solution.

Sample Name Antioxidative effect(%) Barberry Extract (Manufacturing Example 1) 92 Retinol 91 BHT 89

According to the results of Table 1, it was confirmed that the excellent antioxidant effect in all of the sample solution, in particular, the tree extract was found to have an equivalent or somewhat improved antioxidant effect than retinol and BHT.

Example 2 Antioxidant Effect Using DPPH Method

In order to measure the antioxidant effect of the bark tree extract obtained in Preparation Example 1, the antioxidant activity was measured using a DPPH method using a green tea extract and an antioxidant such as vitamin E as a comparative sample under laboratory conditions. In the DPPH method, antioxidant activity was measured by DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical scavenging activity. The free radical scavenging rate was measured at a wavelength of 560 nm by comparing the degree of DPPH reduction by the test substance to the decrease in absorbance. As the reagent used, 61.88 mg of a 0.1 mM solution of DPPH free radicals (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 mL.

The antioxidant activity measurement method by the DPPH method will be described in more detail.

0.15 mL of 0.1 mM DPPH solution and sample solution, 0.15 mL, respectively, were added to the 96-well plate, and the solution was quickly stirred. Absorbance (S _t ) at nm was measured.

For the blank test solution, only distilled water was used instead of the sample solution. The rest of the experiment was performed in the same manner as described above, and then the absorbance at 560 nm (B _t ) was measured.

Merely blank (Blank) of the sample solution was used in place of methanol, 0.15 mL solution of DPPH, and the remaining course of the experiment was measured and the absorbance (B _o) at 560 nm after performing in the same manner as described above.

Substituting the absorbance value measured in the above Equation 2 to calculate the antioxidant activity by the DPPH method, the results are shown in Table 2 below.

In Equation 2, S _t represents absorbance values measured after free radical scavenging of the sample solution, B _t represents absorbance values measured after free radical scavenging of the blank test solution, and S _o represents free radicals in the sample solution. The absorbance value measured before the addition of is indicated, and B _o represents the absorbance value measured before the addition of free radicals to the blank test solution.

Sample Name Antioxidative effect (%) Barberry Extract (Manufacturing Example 1) 67 Green tea extract 35 Vitamin E 33

According to the results of Table 2, it was confirmed that the bark tree extract has an excellent antioxidant effect than the green tea extract and vitamin E.

Example 3. Cytotoxic Alleviation Effect by UV Irradiation

It was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of the barberry extract obtained in Preparation Example 1. Fibroblasts (fibroblasts) were placed in 24-well test plates 1 × 10 ⁵ and attached for 24 hours. Each well was washed once with PBS and 500 uL of PBS was added to each well. The fibroblasts were irradiated with UV 10 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 mL) was added. After processing the bark tree extract to be evaluated here, it was incubated for 24 hours. After 24 hours, the medium was removed, and 500 uL of cell culture medium and 60 uL of MTT solution (2.5 mg / mL) were added to each well, followed by incubation in a 37 ° C. CO ₂ incubator for 2 hours. The medium was removed and 500 uL of isopropanol-HCl (0.04 N) was added. After shaking for 5 minutes to lyse the cells and transfer 100 uL of the supernatant to 96-well test plate, the 565 nm absorbance was measured in a microplate reader. Cell survival rate (%) was measured by Equation 3 below, and cytotoxicity relaxation rate by UV was calculated by Equation 4 below. The results are shown in Table 3 below.

In Equation 3, S _t represents the absorbance value of the well measured after generating and reacting the well treated with the sample, and B _t represents the absorbance value measured after the color reaction of the well not treated with the sample, B _o shows the absorbance value measured after the color reaction of the well which processed the sample.

In Equation 4, S _t represents the cell viability of the wells that are irradiated with ultraviolet light and treated the sample, B _t represents the cell viability of the wells which are irradiated with ultraviolet light and not treated with the sample, and B _o does not irradiate the ultraviolet light, The cell viability of the wells in which the sample was not treated is shown.

Sample Name Cytotoxic alleviation rate (%) Barberry Extract (Manufacturing Example 1) 63

According to the results of Table 3, it can be seen that the bark tree extract effectively prevents ultraviolet rays because it alleviates cytotoxicity by 63%.

Example 4 Inhibitory Effect of Inflammatory Cytokine Expression by Ultraviolet Irradiation

In order to confirm the anti-inflammatory effect of the bark tree extract obtained in Preparation Example 1 was evaluated the degree of inhibition of inflammatory cytokine expression expressed by ultraviolet irradiation.

Fibroblasts isolated from human epidermal tissue (Fibroblast) were placed in a 24-well test plate each 5 × 10 ⁴ and attached for 24 hours. Each well was washed once with PBS and 500 uL of PBS was added to each well. The fibroblasts were irradiated with UV 10 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was removed and FBS was not added to the cell culture medium (DMEM). Medium) 350 uL was added. After processing the bark tree extract to be evaluated here, it was incubated for 5 hours. 150 μL of the culture supernatant was taken to quantify IL-1α, and the inhibitory effect of inflammatory cytokine expression was determined. The amount of IL-1α was quantified using Enzyme-linked Immunosorbent Assay (ELISA), and the production rate of IL-1α was calculated by the following Equation 5. The results are shown in Table 4 below.

In Equation 5, S _t represents the amount of IL-1α produced in the wells that have been irradiated with UV light and the sample is treated, B _t represents the amount of IL-1α produced in the wells not irradiated with UV light, and B _o is The amount of IL-1α produced in the wells not irradiated with ultraviolet light but not treated with the sample is shown.

Sample Name Inhibitory rate of inflammatory cytokine expression (%) Barberry Extract (Manufacturing Example 1) 74

According to the results of Table 4, the bark tree extract is 74% inhibit the production of inflammatory cytokines IL-1α by ultraviolet rays, it can be seen that effectively protects the inflammation caused by ultraviolet rays.

Example 5 Inhibitory Effect of Melanin Production Using B16F1 Melanosite

In order to confirm the skin whitening effect of the bark tree extract obtained in Preparation Examples 1 to 6, the whitening effect was determined by looking at the degree of inhibition of melanin production for B16F1 melanocytes.

B16F1 melanocytes used in this example are cell strains derived from mice, and cells that secrete a black pigment called melanin. The degree of reduction of melanin black pigment was evaluated by treating the sample solution during artificial culture of these cells. The B16F1 melanocytes used in this example were used after being sold from ATCC (American Type Culture Collection, Accession No .: 6323).

The melanin biosynthesis inhibitory effect of B16F1 melanocytes was measured as follows. B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 × 10 ⁶ per well, cells were attached, and the samples were incubated for 72 hours by treating the samples at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation.

Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan: Cancer Res. , 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 mL of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melt the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and measure the absorbance of melanin at 405 nm with a microplate reader, and then quantify the melanin. Melanin production inhibition rate (%) was measured. Melanin inhibition rate (%) of B16F1 melanocytes was calculated by the following equation (6). IC ₅₀ value is the concentration of substance that inhibits melanin production by 50%. The measured IC ₅₀ values are shown in Table 5 below.

In Equation 6, A represents the melanin amount of the well to which the sample is not added, and B represents the melanin amount of the well to which the sample is added.

Sample Name Melanin production inhibitory effect (IC ₅₀ ) Extract of Ethanol aqueous solution of bark tree (Preparation Example 1) 0.06% Bare tree propylene glycol extract (Preparation Example 2) 0.15% Barberry Ethyl Acetate Extract (Preparation 3) 0.18% Barberry Extract (Manufacturing Example 4) 0.13% Bare tree dichloromethane extract (Preparation 5) 0.14% Bare tree ethanol hexane extract (Preparation 6) 0.19% Hydroquinone 0.20% Arbutin 0.15% Lettuce extract 0.19% Usefulness Licorice extract 0.24%

According to the results of Table 5, the IC ₅₀ value of the barberry extract obtained in Preparation Examples 1 to 6 ranges from 0.06 to 0.19%, which is similar to that of the conventional whitening agent hydroquinone, arbutin, oil-soluble licorice extract, and lettuce extract. It can be seen that the excellent effect.

Example 6 Moisturizing Effect (Cream Agent)

The cream form 'mainwon cosmetics 1' containing the extract of the bark tree obtained in Preparation Example 1 and the cream cosmetics 'comparative cosmetics 1a, 1b, 1c, 1d' not containing the bark tree extract were prepared, respectively. The skin moisturizing effect was evaluated by performing a comparative experiment. The composition of the 'main cosmetics 1' and 'comparative cosmetics 1a, 1b, 1c, 12d' is shown in Table 6 below.

The composition (b) shown in Table 6 below was heated and stored at 70 ° C. After adding the composition (A) to preemulsification, the mixture was emulsified uniformly with a homomixer, and then cooled slowly to prepare a cream.

Raw material (% by weight) Cosmetics 1 Comparative cosmetics 1a 1b 1c 1d Furtherance
(end) Stearyl alcohol 8 8 8 8 8 Stearic acid 2 2 2 2 2 Stearic acid cholesterol 2 2 2 2 2 Squalane 4 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 6 Polyoxyethylene (25 mol addition)
Alcohol ester 3 3 3 3 3 Glyceryl Monostearic Acid
ester 2 2 2 2 2 Furtherance
(I) Bare tree extract 5 - - - - glycerin - - 5 - - Na-hyaluronic acid salt - - - 5 - 1,3-butylene glycol - - - - 5 Purified water 68 73 68 68 68 system 100 100 100 100 100

Twenty test subjects (women aged 20 to 35 years old) applied the original cosmetics 1 and the comparative cosmetics 1a, 1b, 1c, and 1d on the lower forearm, and after about 90 minutes, moisturized before and after Ability was assessed by ΔHydration with Corneometer (CM 820).

△ Hydration = Moisturizing after applying-Moisturizing before applying

division Cosmetics 1 Comparative cosmetics 1a 1b 1c 1d Moisturizer Types Bare tree extract - glycerin Na-hyaluronic acid salt 1,3-butylene glycol △ Hydration 17 6 11 14 10

According to the results of Table 7, it can be seen that the moisturizing effect is excellent in the lower forearm of the experimenter applying the cream containing the bark tree extract.

Example 7. Skin Whitening Effect (Cosmetic Water)

Prepares the 'main cosmetics 2' in the form of the lotion containing the bark tree extract obtained in Preparation Example 1 and the 'comparative cosmetics 2' in the form of the lotion without the bark tree extract, respectively, for the skin whitening effect Was evaluated. The composition of the 'main cosmetics 2' and 'comparative cosmetics 2' are shown in Table 8 below.

The composition (A) of Table 8 was exactly weighed out, and the temperature was warmed to 45 ° C. In a separate container, the composition (B) was accurately weighed, dissolved in warming, and slowly mixed with the composition (A) and stirred at 2000 rpm for 2 minutes using a disper mixer to prepare a lotion.

Raw material (% by weight) Cosmetics 2 Comparative Cosmetics 2 Furtherance
(end) Barberry Extract (Manufacturing Example 1) 1.0 - glycerin 3.0 3.0 1,3 butylene glycol 4.0 4.0 Sodium citrate 0.2 0.2 Citric acid 0.1 0.1 Purified water 80.25 81.25 Furtherance
(I) antiseptic 0.4 0.4 Polyoxyethylene (40)
Cured Castor Oil 1.0 1.0 Spices 0.05 0.05 ethanol 10.0 10.0 system 100 100

15 test subjects (women aged 20 to 35 years) were applied to the right side of the face 'main cosmetics 2' and the left side of the face 'comparative cosmetics 2' twice a day for 2 consecutive months. After completion of the test, the applied skin of both sides of the face was measured for change in brightness (ΔL) of color using an image analyzer and skin color change using a chromameter (Minolta CR300).

ΔL = skin brightness after test-skin brightness before test

Objective visual observation by a plurality of skilled workers and subjective visual observation by a subject were performed, and the effects were measured according to the following classification. At this time, the degree of whitening efficacy was classified into the following seven ratings.

[Whitening efficacy evaluation criteria]

-3: very worse -2: worse

-1: slightly worse 0: no change

1: slightly improved 2: improved

3: highly improved

The results are shown in Table 9 below.

division Skin-colored
Change in brightness (ΔL) Objective evaluation of the skilled person Subjective Evaluation of the Subject Cosmetics 3 Comparative Cosmetics3 Cosmetics 3 Comparative Cosmetics3 medium 3.0 2.5 1.5 2.6 1.8

According to the results of Table 9, it can be seen that the whitening effect is excellent in the facial skin of the experimenter applying the lotion containing the bark tree extract.

Example 8. Skin Whitening Effect (Lotion)

The lotion form 'mainwon cosmetics 3' containing the thyme extract obtained in Preparation Example 1 and the lotion form 'comparative cosmetics 3' containing no thyme extract were prepared, respectively, and the skin whitening effect on humans. Was evaluated. The composition of the 'main cosmetics 3' and 'comparative cosmetics 3' is shown in Table 10 below.

The composition (A) of the following Table 10 was exactly weighed, and the temperature was heated up to 80 degreeC. The composition (B) was precisely weighed in a separate container, heated and dissolved, and then slowly mixed with the composition (B), stirred at a rate of 3600 rpm for a predetermined time using a stirrer, and cooled to 25 ° C. to prepare a lotion.

division Raw material (% by weight) Cosmetics 3 Comparative Cosmetics3 Furtherance
(end) Cetearyl Alcohol 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 Ceramide 0.7 0.7 Setares-4 1.2 1.2 Sunflower oil 10.0 10.0 Furtherance
(I) Bare tree extract 3.0 - glycerin 5.0 5.0 Carbomer 0.2 0.2 antiseptic 0.4 0.4 Purified water 76.3 79.3 system 100 100

15 test subjects (women aged 20 to 35 years) were applied with lotion for two months in a row on the right side of the face and 'comparative cosmetic 3' on the left side of the face twice a day. After completion of the test, the applied skin of both sides of the face was measured for change in brightness (ΔL) of color using an image analyzer and skin color change using a chromameter (Minolta CR300).

ΔL = skin brightness after test-skin brightness before test

Objective visual observation by a plurality of skilled workers and subjective visual observation by a subject were performed, and the effects were measured according to the following classification. At this time, the degree of whitening efficacy was classified into the following seven ratings.

[Whitening efficacy evaluation criteria]

-3: very worse -2: worse

-1: slightly worse 0: no change

1: slightly improved 2: improved

3: highly improved

The results are shown in Table 11 below.

division Skin-colored
Change in brightness (ΔL) Objective evaluation of the skilled person Subjective Evaluation of the Subject Cosmetics 3 Comparative Cosmetics3 Cosmetics 3 Comparative Cosmetics3 medium 3.3 2.5 1.9 2.7 1.7

According to the results of Table 11, it can be seen that the whitening effect is excellent in the facial skin of the experimenter applying the lotion containing the bark tree extract.

Example 9. Skin Whitening Effect (Cream)

'Wonwon cosmetics 4' in the form of a cream containing the extract of the bark tree tree obtained in Preparation Example 1 and 'comparative cosmetics 4' in the form of a lotion containing no bark tree extract, respectively, skin whitening effect on human Was evaluated. The composition of the 'main cosmetics 4' and 'comparative cosmetics 4' is shown in Table 12 below.

Raw material (% by weight) Cosmetics 4 Comparative Cosmetics 4 Furtherance
(end) Stearyl alcohol 8.0 8.0 Stearic acid 2.0 2.0 Stearic acid cholesterol 2.0 2.0 Squalane 4.0 4.0 2-octyldodecyl alcohol 6.0 6.0 Polyoxyethylene (25 mol addition) alcohol ester 3.0 3.0 Glyceryl Monostearic Acid Ester 2.0 2.0 Liquid paraffin 5.0 5.0 Furtherance
(I) Bare tree extract 3.0 - Propylene glycol 5.0 5.0 glycerin 4.0 4.0 Purified water 56.0 59.0 system 100 100

15 test subjects (women aged 20 to 35 years) were applied to the right side of the face 'main cosmetics 4' and the left side of the face 'comparative cosmetics 4' twice a day for 2 consecutive months. After completion of the test, the applied skin of both sides of the face was measured for change in brightness (ΔL) of color using an image analyzer and skin color change using a chromameter (Minolta CR300).

ΔL = skin brightness after test-skin brightness before test

Objective visual observation by a plurality of skilled workers and subjective visual observation by a subject were performed, and the effects were measured according to the following classification. At this time, the degree of whitening efficacy was classified into the following seven ratings.

[Whitening efficacy evaluation criteria]

-3: very worse -2: worse

-1: slightly worse 0: no change

1: slightly improved 2: improved

3: highly improved

The results are shown in Table 13 below.

division Skin-colored
Change in brightness (ΔL) Objective evaluation of the skilled person Subjective Evaluation of the Subject Cosmetics 4 Comparative Cosmetics 4 Cosmetics 4 Comparative Cosmetics 4 medium 3.2 2.6 1.8 2.7 1.6

According to the results of Table 13, it can be seen that the whitening effect is excellent in the facial skin of the experimenter applying the cream containing the bark tree extract.

Claims (5)

Sorbaria sorbifolia var. Stellipila extract cosmetic composition for skin whitening, characterized in that it contains. The method according to claim 1, The simple tree extract is a cosmetic composition, characterized in that it contains 0.001 to 30.0% by weight based on the total lyophilized weight. The method according to claim 1 or 2, The tree extract may be purified water, alcohol having 1 to 6 carbon atoms, glycerin, alkylene glycol having 2 to 6 carbon atoms, alkanes having 1 to 6 carbon atoms, halogenated hydrocarbons having 1 to 6 carbon atoms, alkyl alkanoates having 2 to 6 carbon atoms, and Cosmetic composition characterized in that the extraction using the extraction solvent selected from the mixture. The method according to claim 3, The chitan extract is a cosmetic composition, characterized in that extracted using a solvent extracted from purified water, methanol, ethanol, glycerin, ethylene glycol, propylene glycol, butylene glycol, hexane, dichloromethane, dichloroethane, ethyl acetate and mixtures thereof . The method according to claim 1 or 2, Cosmetic composition, characterized in that it is selected from a formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, emulsion in water (O / W) or water-in-oil (W / O) type, and ointment.