KR102253095B1 - Cosmetic composition for improving acned skin, Manufacturing method thereof and functional cosmetics for improving acned skin containing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition having effects of alleviating and preventing acne skin through controlling the amount of sebum secretion in the skin as well as providing whitening and anti-wrinkle effects, a manufacturing method thereof and functional cosmetics for alleviating the acne skin using the same. The cosmetic composition contains vitamin C, adenosine, a combined herbal extract and a solvent.

Description

Cosmetic composition for improving acned skin, Manufacturing method thereof and functional cosmetics for improving acned skin containing the same}

The present invention relates to a cosmetic material, a functional cosmetic having excellent acne skin improvement effect through relieving and/or preventing acne, a method of manufacturing the same, and a functional cosmetic using the same.

In general, acne is an inflammatory disease of the fur sebaceous glands that occurs a lot on a person's face, especially on the cheeks and forehead. It occurs mainly during puberty, declines in the mid-20s, and appears in severe form in men. Various factors are involved in such acne, and symptoms appear due to the interaction of several factors. Factors involved in the occurrence of acne include increased sebum secretion, abnormally increased hair follicle entrance hyperkeratosis and pore obstruction, proliferation of acne bacteria, and inflammatory reactions.

As a method for treating such acne, there is a method of using a drug in the form of an oral or external preparation. Specifically, resocinol, sulfur, salicylic acid, benzoyl peroxide, retinoic acid or isotretinoin, including methods of suppressing sebum production using anti-male hormones, anti-inflammatory effects of steroid hormones and nonsteroidal anti-inflammatory analgesics. There is a method of inhibiting the activity of Propionibacterium acne by antibiotics such as antibacterial agents and exfoliating agents, tetracycline, erythromycin, and macrocycline. On the other hand, treatment methods using azelaic acid are also widely used.

However, although the above-described methods have achieved some degree of achievement, acne treatment is not performed in a short period of time, but requires constant and continuous treatment, so conventional acne treatment methods are only available for a short period of time or use within a limited range. It has several problems in terms of effectiveness, side effects, and usability.

Therefore, the necessity of developing products that are stable and effective for the human body for acne skin is steadily emerging, and in order to meet these practical needs, recently, the side effects can be reduced from natural resources including plants and used continuously. Research is actively being conducted to search for active ingredients that are harmless to the human body and have a strong anti-acne effect.

In particular, studies to improve acne using herbal medicinal herbs and natural plant materials are being actively conducted in Korea. Herbal ingredients are used as raw materials for functional cosmetics and soaps related to acne treatment, and development of cosmetic raw materials for anti-acne bacteria using chemical substances derived from natural substances is also underway.

Korean Patent Publication No. 10-2017-0090883 (published on August 8, 2017) Korean Patent Application Publication No. 2000-0049163 (published on July 25, 2000) Korean Patent Application Publication No. 10-2004-0090644 (published on October 26, 2004)

As a result of trying to develop a product having an effect of improving acne skin while preventing and minimizing skin side effects, the present invention was completed by knowing the acne skin improvement cosmetic material and the optimal composition ratio thereof. That is, an object of the present invention is to provide a cosmetic for improving acne skin using the above composition, a method for preparing the same, and a functional cosmetic using the same.

In order to solve the above problems, the acne skin improvement cosmetic of the present invention includes a mixture of vitamin C, adenosine, and herbal complex extract.

In addition, an object of the present invention is to provide a method of manufacturing the acne skin improvement cosmetic.

In addition, another object of the present invention is to provide a functional cosmetic using a cosmetic for improving acne skin.

The acne skin improvement cosmetic of the present invention has excellent anti-oxidation, whitening, and wrinkle-improving effects, as well as acne alleviation and/or prevention effect through improvement of skin sebum secretion, but does not cause skin troubles. Cosmetics can be manufactured.

Hereinafter, it will be described in more detail through the method of manufacturing the acne skin improvement cosmetic of the present invention.

The cosmetic composition of the present invention comprises a first step of preparing an herbal complex extract; The herbal complex extract, vitamin C, adenosine, and a compatibilizer are added to a solvent, followed by stirring to prepare a cosmetic.

A method of preparing the herbal complex extract in step 1 will be described as follows.

The herbal complex extract includes Mugwort, Green Tea Leaf, Peony Root, Centella asiatica, Spanish Licorice Root, Yakmomile, Ginseng Root, Mud Mushroom, Golden Root, Tang Elm Root, Raspberry Leaf, Mulberry Leaf, Western Pine Leaf and Pine Tree Leaf. 1-1 step of preparing a mixed dried product; 1-2 steps of extracting the dried product from alcohol, hot water extraction, or steam distillation to obtain an extract; And 1-3 steps of filtering the alcohol extract or hot water extract to obtain a filtrate.

The herbs (herb) materials in step 1-1 are dried and may be in the form of non-powder or powder.

And, the dry mixture is based on dry weight, based on 100 parts by weight of wormwood, green tea leaves 40 to 60 parts by weight, peony root 10 to 40 parts by weight, centella leaves 50 to 80 parts by weight, Spanish licorice root 120 to 150 parts by weight Part, Yakushi 50 ~ 150 parts by weight, Ginseng root 1 ~ 10 parts by weight, Mud mushroom 5 ~ 20 parts by weight, Golden root 30 ~ 50 parts by weight, Sugar elm root 100 ~ 150 parts by weight, Raspberry leaves 20 ~ 50 parts by weight , Mulberry leaves 10 to 30 parts by weight, Western pine needles 10 to 30 parts by weight, and pine leaves 10 to 30 parts by weight, preferably based on dry weight, based on 100 parts by weight of wormwood, green tea leaves 45 to 55 parts by weight, 10 to 30 parts by weight of peony root, 60 to 75 parts by weight of Centella asiatica, 120 to 140 parts by weight of Spanish licorice root, 100 to 130 parts by weight of yam, 1 to 5 parts by weight of ginseng root, 8 to 17 parts by weight of mud mushrooms Parts, golden root 30 ~ 40 parts by weight, sugar elm root 100 ~ 120 parts by weight, raspberry leaves 20 ~ 35 parts by weight, mulberry leaves 12 ~ 25 parts by weight, western pine needles 12 ~ 30 parts by weight and pine leaves 15 ~ 30 May contain parts by weight, more preferably, based on dry weight, based on 100 parts by weight of wormwood, green tea leaves 50 to 55 parts by weight, peony root 10 to 25 parts by weight, centella leaves 60 to 70 parts by weight, Spanish licorice 125 to 140 parts by weight of root, 110 to 130 parts by weight of yam, 1.5 to 4.5 parts by weight of ginseng root, 10 to 15 parts by weight of mud mushroom, 30 to 35 parts by weight of golden root, 100 to 115 parts by weight of sugar elm root, raspberry leaf It may contain 20 to 25 parts by weight, 12 to 20 parts by weight of mulberry leaves, 15 to 22 parts by weight of western pine needles, and 20 to 30 parts by weight of pine leaves.

Artemisia annua L. is a plant distributed mainly in temperate, cold and subtropical regions of Asia, Europe and North Africa. Artemisia annua L. has various ingredients such as essential oil, sesquiterpene lactone, flavonoids, coumarin, etc. It is a plant that is known to have antipyretic, anti-inflammatory, analgesic, antimalarial, anti-hemorrhagic, immunomodulatory, antitumor, antibacterial, and antiviral effects.

Green tea is a medicinal use of green tea leaves, and it contains a large amount of tannins and saponins, and is widely known to be effective in arousal, diuretic, detoxifying, anti-inflammatory, and sterilizing effects.

The peony (Paeonia suffruticosa) is also called Mokdan (牧丹), has a height of 2m and is cultivated in various places. And, the branch is thick and has no hairs, the leaves are in 3 layers, the small leaves are egg-shaped, divided into 2 to 5, the surface of the leaves has no hairs, and the back side has fine hairs, and is often white. The present invention uses the roots of these peonies, and uses the roots of the peony as an anti-inflammatory component.

Centella asiatica is a perennial plant of the Ranunculus family, and grows in a somewhat humid place. As it extends to the side, roots descend from the nodes and have scale-like leaves, and normal leaves come from scale-like leaf axils, have long stalks, ovals, 2-5cm in diameter, and have dull serrations at the edges. Centella asiatica is used for medicinal purposes in India, and is a poisonous plant when taken in large quantities.

Spanish liquorice (licorice) is a perennial plant of the rosewood legume family, a herb native to the Mediterranean coast and western Asia, a medicinal plant since hundreds of years BC, and is known as a medicinal plant in India and China. Its scent is similar to anise or fennel, but it is slightly stronger and tastes very sweet. It grows to 1.5 ~ 2m in height, and the rootstock is strong, and the creeper stem comes out from here, reaching 2m in length. Roots come out from the creeper stem again, and the outside of the roots and roots are brown, and the inside is yellow, and the taste is sweet. The leaves are feather-shaped bileaflets (multiple leaves) consisting of 2 to 8 small leaves each. The root of the Spanish licorice of the present invention is used, and contains a large amount of glycyrrhizin and flavin components.

Houttuynia cordata, also known as Eoseongcho, grows in a shady forest, and the subterranean stem is long, thin and white. The stem stands upright, has a height of 20 ~ 50cm, has several vertical lines, leaves are alternate, 3 ~ 8cm long, has a pointed end, flat edges, and a stipule is attached to the bottom of the petiole. In oriental medicine, plants are also used for gonorrhea, enteritis, urinary tract infections, pneumonia, and bronchitis. In the present invention, the use of yakmoil serves to reduce the toxicity of centella asiatica.

Mud mushrooms ( Phellinus igniarius (L.) Quel.) are various types such as woody mud mushrooms, dry mud mushrooms, horse dung mud mushrooms, plaster mud mushrooms, flat mud mushrooms, yellow mud mushrooms, and longevity mud mushrooms, and are used in the present invention. The type of mud mushroom to be used is not particularly limited, but preferably, wood mud mushroom, dry mud mushroom and/or flat mud mushroom may be used.

Gold (Scutellaria baicalensis) is distributed in Korea, China, Mongolia, and eastern Siberia, and uses the root of gold in oriental medicine as antipyretic, diuretic, branch, sputum, and anti-inflammatory. The present invention uses the golden root as an antibacterial component.

Ulmus davidiana, also known as Tangyu (唐楡) or Heukyu (黑楡), grows at the foot of a mountain or in a valley. The height is 15m, the diameter of the base is 50 ~ 70cm, the small branches have long soft hairs, and the bark is light gray. The leaves are alternate, 4 to 8 cm long, in the shape of an inverted ellipse or a long ellipse. The tip of the leaf becomes sharper and the bottom is distorted, there are sharp double teeth on the edge, the front surface is slightly rough, and there are hairs on the vein on the back. The bark or root bark of the Tang Elm tree is referred to as cystic yupi, and the foliage is referred to as cystic euphorbia, and the present invention uses the root of the Tang Elm tree.

Raspberry leaves are mostly distributed and cultivated in Europe and North America, and are sweet and juicy, and classified into three types of raspberry leaves according to their color. Smelling the scent has the effect of decomposing fat and suppressing appetite, and black raspberry leaves are known to have an effect of suppressing the occurrence of esophageal cancer. The present invention uses the leaves of these rabberries as an antioxidant component.

White Mulberry is known to contain a large amount of flavone compounds and scopoletin, and pharmacologically, it is known to have diuretic, blood sugar lowering, anti-inflammatory, antibacterial, and antiviral effects. In the present invention, the leaves of the mulberry tree contain a large amount of flavonoid components and alkaloid components, and the present invention uses the leaves of the mulberry tree as an anti-inflammatory and antibacterial component of a cosmetic.

Western songak (Hedera helix) is widely distributed in Europe and North Africa, and was introduced as a houseplant in Korea and distributed under the name of helix. In the native place, the height is up to 30m, the leaves are alternate, glossy dark green, 10cm long, split into 3 to 5 branches, and the leaves of the old branches are egg-shaped without cracking. The present invention uses such a leaf of Western pine, and is used for a skin soothing effect together with a pine leaf.

In the herbal complex extract manufacturing process of the present invention, step 1-2 is a process of obtaining an extract by extracting the dried product of step 1-1 by alcohol extraction, hot water extraction, or steam distillation extraction.

The alcohol extraction can be performed as a general alcohol extraction process used in the art. For example, the mixed dried product of step 1-1 is extracted with lower alcohols such as methanol, ethanol, and butylene glycol at 4 to 40°C. It may be performed by immersing in a solvent for 1 to 20 hours, and for a more preferable example, the mixed dried product may be immersed in an aqueous ethanol solution of 35 to 50° C. for 12 to 18 hours to obtain an alcohol extract.

The hot water extraction can be performed as a general hot water extraction process used in the art. For example, after adding the dried mixture of step 1-1 to water, heat extraction at 40-100° C. for 2-20 hours It can be obtained, and for a more preferred example, it can be heated under 70 ~ 90 ℃ for 2 ~ 6 hours to obtain a hot water extract.

In addition, the steam distillation extraction refers to extracting by injecting water into an essential oil extractor and operating it, and the mixed dried product of step 1-1 is heated at 80 to 100°C for 4 to 6 days, preferably Can be distilled in a bath for 4.5 to 6 days under 85 to 950° C. to obtain a steam distilled extract in the form of essential oil.

In the herbal complex extract manufacturing process of the present invention, steps 1-3 are a process of removing impurities from the extract obtained in steps 1-2, and a filtrate removed from impurities is obtained through a general filtration method used in the art. can do.

In the herbal complex extract manufacturing process of the present invention, steps 1-4 are a process of concentrating the filtered extract, and the filtrate of steps 1-3 is concentrated under reduced pressure to obtain a concentrated extract (concentrate). At this time, the concentration under reduced pressure can be carried out through a general method used in the art, and a preferred example is, for example, the filtrate of steps 1-3 is added to a vacuum concentrator, and at least one selected from water, ethanol, and ethyl acetate is extracted. Using a solvent, it is possible to obtain a concentrated extract (concentrate) by concentrating under reduced pressure at 30 to 50°C, preferably at 35 to 48°C. In addition, the vacuum concentration in steps 1-4 is preferably performed when the extract in steps 1-2 is an alcohol extract or a hot water extract, and may not be performed in the case of a steam distillation extract.

The herbal complex extract obtained through steps 1-1 to 1-3 or 1-1 to 1-4 is freeze-dried and powdered as necessary, step 1-5; powdered by performing a process further comprising You can also make it.

The herbal complex extract prepared by the method described above not only increases the antioxidant effect of vitamin C and/or increases the skin aging and wrinkle prevention effect of adenosine, but also the herbal complex extract improves acne with antibacterial, anti-inflammatory, skin soothing effects, etc. It can impart a preventive effect.

Next, in the cosmetic preparation method of the present invention, after the herbal complex extract prepared in step 1 is added to a solvent, vitamin C, adenosine, and a compatibilizer are added, and then heated to 50 to 80°C, preferably 60 to 70°C. It can be prepared by performing a; step 2 of preparing a cosmetic by stirring slowly.

At this time, if the temperature is less than 50°C, mixing between the compositions may be difficult, and if the temperature exceeds 80°C, there may be a problem in the stability of the formulation, so it is recommended to slowly stir after heating to the above temperature range.

The vitamin C is one of the most easily accessible vitamins that are contained in almost all foods, and acts as a strong reducing agent, such as activating the synthase of collagen. Vitamin C has a strong antioxidant function, promotes collagen, suppresses melanin production, and has anti-inflammatory effects, so it reduces wrinkles, makes skin elastic, and has excellent effects in whitening and relieving inflammation. These vitamin C can be ingested through food or can be absorbed directly into the skin.When ingested, the amount reaching the skin is insignificant, and it is excreted within about 3 hours after absorption, so it is more effective to absorb it directly into the skin than ingestion. .

In addition, the content of vitamin C in the cosmetic may include 0.1 to 3.5% by weight, preferably 0.5 to 2.5% by weight, and more preferably 0.7 to 2.0% by weight of the total weight of the cosmetic. At this time, if the vitamin C content is less than 0.1% by weight, the antioxidant effect and skin tone improvement effect may be insufficient, and even if it is used in excess of 2.0% by weight, the increase in the skin improvement effect due to the use of vitamin C is insufficient. It is good.

In addition, adenosine is a nucleoside involved in signal transduction outside the cell, and is directly involved in cell growth, differentiation, and homeostasis. The adenosine is produced in the energy metabolism step, binds to the adenosine receptor connected to the outside of the cell, and undergoes a biological signal transduction process through AC (adenyl cyclase) and PLC (phospholipase C). Finally expresses genes involved in cell differentiation, anti-inflammatory, and wound healing, thereby improving skin. Such adenosine is effective in preventing skin aging and wrinkles, and because it is an intracellular component, it has excellent stability and longevity when penetrating the skin. In addition, unlike retinol, adenosine is a raw material for wrinkle improvement notice by the KFDA that can be used without distinction between night and day.

In addition, the content of adenosine in the cosmetic may include 2.0 to 5.0% by weight, preferably 2.2 to 4.0% by weight, and more preferably 2.2 to 3.2% by weight of the total weight of the cosmetic. At this time, if the adenosine content is less than 2.0% by weight, the effect of preventing aging and wrinkles of the skin may be insufficient, and if it exceeds 5.0% by weight, it may rather cause skin trouble, so it is recommended to use within the above range.

In addition, the content of the herbal complex extract in the cosmetic may be 75 to 90% by weight, preferably 78 to 86.5% by weight, more preferably 80 to 86.5% by weight, based on the total weight of the cosmetic composition, and acne improvement and other skin improvement effects It is preferable to use it within the above range from the side.

In addition, among the cosmetic ingredients, the compatibilizer serves to increase the compatibility and compatibility between vitamin C, adenosine, and herbal complex extract so that it is evenly dissolved in a solvent. As the compatibilizer, polyoxyethylene hydrogenated castor oil may be used, preferably PEG-10 hydrogenated castor oil, PEG-20 hydrogenated castor oil, PEG (polyethyleneglycol)-25 hydrogenated castor oil, PEG-30 hydrogenated castor oil, PEG It is recommended to use at least one polyoxyethylene hydrogenated castor oil selected from -40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-80 hydrogenated castor oil and PEG-100 hydrogenated castor oil, More preferably, at least one selected from PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, PEG-60 hydrogenated castor oil, and PEG-80 hydrogenated castor oil may be used. In addition, the content of the compatibilizer is 0.1 to 2.0% by weight, preferably 0.3 to 1.8% by weight, more preferably 0.5 to 1.2% by weight of the total weight of the cosmetic. At this time, if the content of the compatibilizer is less than 0.1% by weight, there may be a problem that the composition is not evenly distributed and not well dissolved in the solvent due to poor compatibility between cosmetic compositions.If it is used in excess of 2.0% by weight, it may cause skin troubles. Therefore, it is recommended to use it within the above range.

The solvent is water, preferably distilled or purified water, and the content is the remainder of the total weight of the cosmetic composition excluding vitamin C, adenosine, compatibilizer, and herbal complex extract.

The cosmetic composition of the present invention may further include an additive including at least one selected from a complex oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a preservative, and a fragrance.

Among the additives, the complex oil is used to increase the compatibility and miscibility between different compositions, so that the active ingredient of the herbal complex extract is well dissolved in a solvent, and may serve as an auxiliary compatibilizer. The complex oil may be one or more selected from sweet almond (Prunus Amygdalus Dulcis) oil, jojoba seed (Simmondsia Chinensis) oil, and rose hip seed oil, preferably based on 100 parts by weight of sweet almond oil , Jojoba seed oil 20 to 45 parts by weight and rosehip seed oil 10 to 40 parts by weight may be mixed and used, and more preferably, based on 100 parts by weight of sweet almond oil, jojoba seed oil 20 to 30 parts by weight and rosehip seed oil 25 It can be used by mixing ~ 30 parts by weight.

In addition, the content of the composite oil may include 0.05 to 1.5% by weight, preferably 0.1 to 1.2% by weight, and more preferably 0.3 to 1.0% by weight of the total weight of the cosmetic. At this time, exceeding 1.5% by weight may increase the viscosity of the cosmetic as excessive use, so it is preferable to use it within the above range.

Among the additives, the moisturizing agent may be a general moisturizing ingredient used as a cosmetic material, preferably glycerin, caprylic/capric triglyceride, butylene glycol, propane diol, pentylene glycol, Sodium bulinate (Sodium Levulinate), hydrogenated lecithin (Hydrogenated Lecithin), sodium hyaluronate (Sodium Hyaluronate) and ceramide NP (Ceramide NP) may contain at least one selected from, preferably glycerin, butyl Ren glycol, propane diol, sodium fluorinate, hydrogenated lecithin, sodium hyaluronate, and ceramide NP.

And, the amount of moisturizer is 1.0 to 4.0% by weight, preferably 1.5 to 3.8% by weight, more preferably 2.0 to 3.5% by weight of the total weight of the cosmetic, and if the amount of the moisturizer is less than 1.0% by weight, moisturizing The effect may be insufficient, and if it is used in excess of 4.0% by weight, the viscosity of the atopic dermatitis improving agent may become too high, and miscibility with other compositions may be sharply decreased.

Among the additives, the skin function improving agent may include niacinamide and/or madecassoside. Here, the niacinamide is used to obtain an effect of increasing skin whitening, relieving skin inflammation, and strengthening skin barrier by inhibiting the movement of melanin pigment. Madecassoside is used for promoting skin collagen synthesis, increasing skin elasticity, restructuring the extracellular matrix of the dermal layer, and soothing the skin. And, when all three types of niacinamide and madecassoside are used, it is preferable to use niacinamide and madecassoside in a weight ratio of 1:0.3 to 0.6, preferably niacinamide and madecassoside in a weight ratio of 1:0.32 to 0.50. good.

In addition, the amount of the skin function improving agent is preferably 0.1 to 2.0% by weight, preferably 0.2 to 1.8% by weight, and more preferably 0.40 to 1.50% by weight, based on the total weight of the cosmetic composition, and the content of the skin function improving agent is 0.1% by weight. If it is less than %, the amount of use thereof is too small, and the effect of using it may be insufficient. If it is used in excess of 2.0% by weight, it is recommended to use it within the above range because excessive use may cause skin troubles.

Among the additives, the pH adjusting agent is for adjusting the overall pH of the cosmetic, and as long as it does not affect the skin and does not lower the acne improvement effect of the present invention, a pH adjuster used in the art may be used. In addition, the content of the pH adjusting agent is 0.01 to 0.50% by weight, preferably 0.05 to 0.40% by weight, more preferably 0.05 to 0.30% by weight of the total weight of the composition is suitable for maintaining an appropriate pH.

Among the additives, the preservative may be a general preservative that is acceptable as a cosmetic ingredient, and preferably, glyceryl caprylate as a preservative is 0.01 to 0.30% by weight, preferably 0.01 to 0.25% by weight, more preferably, based on the total weight of the cosmetic. It is recommended to use about 0.01 to 0.15% by weight.

In addition, the perfume may be used in a small amount of general perfumes used in the art, preferably 0.3% by weight or less, more preferably 0.05 to 0.25% by weight of the total weight of the composition.

The cosmetic composition for improving acne skin of the present invention prepared by the method described above includes the herbal complex extract, mugwort extract, green tea leaf extract, peony root extract, centella asiatica extract, Spanish licorice root extract, yam extract, ginseng root extract, mud mushroom extract, Herbal complex extracts including golden root extract, dango elm root extract, raspberry leaf extract, mulberry leaf extract, western pine tree leaf extract, and pine leaf extract; Vitamin C, adenosine, and a solvent are included, and if necessary, a compatibilizer and/or the additive may be further included.

Cosmetics of various formulations can be manufactured by using the acne skin improvement cosmetic of the present invention, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition. Cream, moisture cream, hand cream, sun cream, foundation, essence, nutrition essence, pack, soap, shampoo, conditioner, hair treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser. Cosmetics can be manufactured.

The atopic dermatitis improving agent of the present invention prepared by the above manufacturing method using the above composition has excellent skin soothing effect, does not cause skin troubles such as allergies, and has a skin improvement effect, especially atopic dermatitis improvement effect. Is very excellent. The atopic dermatitis improving agent of the present invention can be applied as a material in various formulations of cosmetics, hair products such as shampoos, conditioners, and ointments.

Hereinafter, the present invention will be described in more detail through examples, but the following examples do not limit the scope of the present invention, which should be construed to aid understanding of the present invention.

[Example]

Preparation Example 1: Preparation of herbal complex extract

Dried Mugwort, Dried Green Tea Leaves, Dried Peony Roots, Dried Centella asiatica, Dried Spanish Licorice Root, Dried Yakko Wheat, Dried Ginseng Root, Dried Mud Mushroom, Dried Golden Root, Dried Dandelion Elm Roots, dried raspberry leaves, dried mulberry leaves, dried Western pine leaves, and dried pine leaves were prepared, respectively.

Next, the dried products were mixed in the composition ratio shown in Table 1 below, and then pulverized to prepare a pulverized product.

Next, the pulverized product was mixed with 7L of water, and then distilled in a bath for 5 days at a temperature of 90°C to obtain a steam distilled extract.

Next, the steam distilled extract was filtered to obtain a filtrate to obtain a final herbal complex extract.

Preparation Examples 2 to 3 and Comparative Preparation Examples 1 to 3

The herbal composite extract was prepared in the same manner as in Preparation Example 1, but each of the pulverized products prepared with the composition shown in Table 1 below was steam-distilled and filtered to obtain the herbal composite extracts, respectively, and Preparation Examples 2 to 3 and Comparative Preparation Examples 1 to 3 were carried out, respectively.

division
(Parts by weight based on dry weight) Preparation Example 1 Preparation Example 2 Preparation Example 3 compare
Preparation Example 1 compare
Preparation Example 2 compare
Preparation Example 3 Wormwood 100 100 100 100 100 100 Green tea leaves 53.2 58.3 53.2 53.2 53.2 53.2 Peony root 17.8 12.5 25 17.8 17.8 17.8 Centella asiatica 64.5 58.9 67.2 64.5 64.5 95 Spanish licorice root 130 135 135 130 130 50 Yakmomil 118 100 110 118 118 60 Ginseng root 3.5 3.5 3.5 3.5 3.5 3.5 Mud mushroom 12.5 12.5 10.9 12.5 12.5 12.5 Golden root 32.1 34.8 31.5 32.1 32.1 32.1 Angelic Elm Root 100 100 105 - 70 100 Raspberry leaves 22 25 20.8 - 15 22 Mulberry leaves 16.5 20 17.1 - 10 16.5 Western pine needles 20 17 18.5 - 5 20 Pine leaves 25 23 22.3 - 5 25

Example 1: Preparation of acne skin improvement cosmetic

85.11% by weight of the herbal complex extract of Preparation Example 1, 1.34% by weight of vitamin C, 2.46% by weight of adenosine, 0.63% by weight of PEG-50 hydrogenated castor oil as a compatibilizer, and the remaining amount of purified water were added to the reactor and then heated to about 62°C. Then, it was slowly stirred for 2 hours to prepare a cosmetic.

Examples 2 to 6 and Comparative Examples 1 to 6

A cosmetic composition was prepared in the same manner as in Example 1, but by using different herbal complex extracts as shown in Table 2 below, or each cosmetic composition was prepared by varying the cosmetic composition ratio, Examples 2 to 6 and Comparative Examples 1 to Comparative Example 6 was carried out respectively.

Classification (% by weight) Herbal complex extract Vitamin C Adenosine Compatibilizer menstruum Example 1 Preparation Example 1 85.11 1.34 2.46 0.63 Remainder
Balance Example 2 Preparation Example 2 85.11 1.34 2.46 0.63 Example 3 Preparation Example 3 85.11 1.34 2.46 0.63 Example 4 Preparation Example 1 85.11 2.50 2.46 0.63 Example 5 Preparation Example 3 85.11 1.34 4.00 0.63 Example 6 Preparation Example 2 85.11 1.34 2.46 1.52 Comparative Example 1 Comparative Preparation Example 1 85.11 1.34 2.46 0.63 Comparative Example 2 Comparative Preparation Example 2 85.11 1.34 2.46 0.63 Comparative Example 3 Comparative Preparation Example 3 85.11 1.34 2.46 0.63 Comparative Example 4 Comparative Preparation Example 1 85.11 4.00 2.46 0.63 Comparative Example 5 Comparative Preparation Example 1 85.11 1.34 5.20 0.63 Comparative Example 6 Comparative Preparation Example 1 85.11 1.34 2.46 2.18

Comparative Example 7

After preparing 100 g of each of chamomile, rosemary, lavender, tea tree, cypress, sage, and hibiscus as a dry weight, it was added to 5 L of water and distilled in a bath for 5 days at a temperature of about 90° C. to obtain a herbal complex extract.

A cosmetic was prepared by mixing 85.72% by weight of the obtained herbal complex extract, 7.14% by weight of vitamin C, and 7.14% by weight of adenosine.

Experimental Example 1: Free radical scavenging efficacy experiment

A stable 1,1-diphenyl-2-picrylhydrazyl radical was used as an improvement of the method of Fujita et al. by utilizing the samples of the above examples and comparative examples. (MS Blois, Antioxidant determinations by the use of astable free radical, Nature, 29, pp1199-1200, 1958) Sample 2 ml for each concentration in 1 ml of 0.2 mM diphenyl-2-picrylhydrazyl (DPPH) solution After adding and reacting for 10 minutes at room temperature, the maximum absorbance was measured at 525 nm to measure the free radical scavenging effect (%). And, the free radical scavenging ability was calculated as shown in Equation 1 below, and the measurement results are shown in Table 3 below.

[Equation 1]

Free radical scavenging ability (%)=[1-(A-A')/(B-B')]×100%

A: Absorbance at 525 nm of the reaction solution to which the sample was added.

A': absorbance at 525 nm of the reaction solution without adding DDPH.

B: Absorbance at 525 nm of the reaction solution to which no sample was added.

B': absorbance at 525 nm of the sample and the reaction solution without DDPH added.

Experimental Example 2: Inhibitory effect of tyrosinase activity (Tyrosinase inhibitory activity)

The inhibitory activity of tyrosinase was measured. 0.1 M potassium phosphate buffer (pH 6.8) 1.0 ml, 0.3 mg/ml L-tyrosine aqueous solution 1.0 ml, 1250 units/ml mushroom tyrosinase 0.1 ml mixed After that, 0.2 ml of each sample was added thereto according to the concentration, and the enzyme reaction was carried out at 37°C for 10 minutes. The absorbance of the reaction solution was measured at 480 nm to measure the enzyme inhibitory activity of the sample according to Equation 2 below, and the results are shown in Table 3 below.

[Equation 2]

Tyrosinase inhibition rate (%) = [(A-B)/A]×100%

A: Absorbance at 475 nm of the reaction solution without adding the sample

B: absorbance at 475 nm of the reaction solution to which the sample was added

Experimental Example 3: Elastase inhibition effect experiment

The inhibitory effect of elastase activity was measured. Substrate solution containing 8.8 mM of elastase substrate Succ-Ala-Ala-Ala-p-nitroanilide standard in 60 μl of a buffer solution adjusted with 0.267 M hydrochloric acid (HCl) solution to a pH of 8.0. Μl, each sample prepared by diluting each sample in purified water to a certain concentration, was added to each 100µl and mixed, and 20µl of an enzyme solution of 10µg/ml of Porcine Pancreatic Elastase was added and mixed. After reacting at 25° C. for 15 minutes, the amount of production of para-nitroaniline was measured at a wavelength of 410 nm, and absorbance B was measured. In addition, 20 µl of substrate solution, 20 µl of purified water, and 100 µl of each sample prepared by diluting each sample in purified water to a certain concentration in 60 µl of the same buffer solution as described above were obtained by performing the same method as above, and then used as a control solution. The absorbance C was measured. In addition, the absorbance A was measured using 100 µl of purified water instead of the above sample and using the same method as the sample solution as a blank test solution, and 120 µl of purified water was added instead of the enzyme solution and the sample solution, and the solution was obtained by the same method as the sample solution. As a color correcting solution, the absorbance D was measured, and the elastase inhibition rate (%) was calculated as shown in Equation 3 below, and the results are shown in Table 3 below.

[Equation 3]

Elastase inhibition rate (%) = [(B-C)/(A-D)]×100%

division Free radical scavenging ability (%) Tyrosinase inhibition rate (%) Elastase inhibition rate (%) Example 1 98.312 93.862 88.248 Example 2 98.524 94.213 88.104 Example 3 98.851 95.337 87.230 Example 4 98.650 94.208 88.450 Example 5 97.151 94.291 88.629 Example 6 98.355 94.089 88.851 Comparative Example 1 90.853 90.723 80.553 Comparative Example 2 92.532 91.830 82.792 Comparative Example 3 94.238 92.284 83.985 Comparative Example 4 98.652 94.210 86.382 Comparative Example 5 97.109 94.331 88.517 Comparative Example 6 98.489 94.107 88.640 Comparative Example 7 97.143 92.187 85.429

Looking at Table 3, when compared to Comparative Example 7, which is a conventional cosmetic product, the cosmetics of Examples 1 to 6 are relatively high in all of the free radical scavenging ability, the tyrosinase inhibition rate, and the elastase inhibition rate despite the low use of vitamin C and adenosine. Showed.

In the case of Comparative Preparation Example 1 and Comparative Preparation Example 2 in which the five kinds of herbs were not used, such as the sugar elm root, and the contents of the five kinds of herbs were small, as compared with Examples 1 to 6, the overall free radical scavenging ability, tyrosinase inhibition rate And elastase inhibition rate was low, and a result was relatively lower than that of Comparative Example 7. Through this, it was confirmed that there is a synergistic effect in terms of antioxidant and whitening effects by using together 5 kinds of herbs such as the dandelion tree root.

In the case of Comparative Example 3 using the herbal composite extract of Comparative Preparation Example 3, as compared with Examples 1 to 6, the free radical scavenging ability and the elastase inhibition rate were decreased.

In addition, in the case of Comparative Example 4 (4% by weight) using an excess of 3.5% by weight of vitamin C, as compared with Example 1 (1.34% by weight) and Example 4 (2.5% by weight), free radical scavenging ability and tyrosinase inhibition rate increased. There was a problem that the inhibition rate of elastase was lowered rather than having an effect.

And, in the case of Comparative Example 5 (5.2% by weight) in which more than 5% by weight of adenosine was used, there was no increase in the overall effect as compared with Example 1 (2.46% by weight) and Example 5 (4% by weight). In addition, in the case of Comparative Example 6 (2.18% by weight) in which the compatibilizer was used in excess of 2% by weight, there was no increase in the effect as compared with Example 1 (0.63% by weight) and Example 6 (1.52% by weight).

Experimental Example 4: Measurement test of the effect of inhibiting skin sebum secretion

(1) Human keratinocytes, HaCaT (GIBCO, product number HEKa (C-005-5C)) cells were aliquoted into a 6-well plate at a ^{concentration of 5×10 5} cells/well, respectively, and then 10% by volume into each well. Fetal bovine serum (GIBCO, product number 12483-020), 100 IU/ml penicillin (SIGMA, product number M5655) and 100 μg/ml streptomycin (SIGMA, product number S9137) in DEME medium (Wellgene, product No. LM001-51) was added and _{cultured for 2 days at 37° C., under 5% CO 2} (hereinafter, the same conditions for cell culture).

(2) Dihydrotestosterone (Sigma, Example 1, Example 2, Comparative Example 3, Comparative Example 7 and Control Example) for inducing sebum secretion with washing the cultured HaCaT cells with PBS and replacing with the same DEME medium Product No. D-073) was treated together and incubated for 3 days at _{37°C and 5% CO 2.}

(3) For analysis of anti-inflammatory genes from cultured cells, RNA was extracted and quantified in the following manner, and then cDNA was synthesized to perform RT-PCR (Reverse transcription-polymerase chain reaction).

(4) cDNA synthesis was performed using the PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, Cat. No 6110A), and an experiment was performed by the method of the kit. PCR was performed according to the method of the kit using Taq polymerase Kit (TaKaRa, Cat. No R001A), and the following Table 4 using PCR equipment (Biofree, Cat. No TC-96/G/H/(b)B) The gene was amplified with the primers of. The product produced by PCR was electrophoresed on a 1% agarose gel and confirmed with an image analyzer (KOREALABTECH, Bundang, Korea; product number DGS-200D), and a gel documentation system (KOREALABTECH, Bundang, Korea). ; Product number DGS-200D) and the image J program (National Institutes of Health; obtained through the website [imagej.net]) by analyzing the expression amount of SCD-1 (stearoyl-CoA desaturase-1) in Table 5 below. Indicated.

[Equation 4]

Gene expression rate (%) = (gene expression amount during sample treatment/control gene expression amount) × 100%

primer order Where to get it SCD-1 Forward: 5'-CCG GGA GAA TAT CCT GGT TT-3' (SEQ ID NO: 3) Reverse: 5'-GCG GTA CTC ACT GGC AGA GT-3' (SEQ ID NO: 4) Bioneer

division SCD-1 expression rate (%) Control 100% Example 1 68.81% Example 2 63.76% Comparative Example 3 89.01% Comparative Example 7 97.42%

Looking at the measurement results in Table 5, in the case of Comparative Example 7, the SCD-1 expression rate was very high, which was confirmed that the effect of suppressing skin sebum secretion was insufficient, and thus the effect of improving acne was small.

And, in the case of Comparative Example 3, which is a cosmetic composition using the herbal composite extract of Comparative Preparation Example 3, there was a problem in that the SCD-1 expression rate was higher than that of Examples 1 to 2.

On the other hand, in the case of Examples 1 to 2, it was confirmed that the SCD-1 expression rate was 80% or less, preferably 70% or less, and through this, it was excellent in suppressing the secretion of skin sebum, and through this, it was confirmed that there is an effect of improving acne. Could.

Experimental Example 2: Evaluation of adverse skin reactions

After using the cosmetics of Example 1, Example 5, Comparative Example 5, and Comparative Example 6 for 2 weeks, a questionnaire evaluation was conducted to determine whether abnormal skin reactions occurred.

The criteria for selecting test subjects are shown in Table 6, and the questionnaire evaluation on the skin condition of the test subjects before the evaluation of 20 subjects is shown in Table 7 below.

In addition, in the evaluation of adverse skin reactions and evaluation of the feeling of use, the results of a questionnaire survey after each of 20 test subjects used the cosmetics of Example 1, Example 5, and Comparative Examples 5 and 6 for 2 weeks are shown in Table 8 below. Dog samples were used for 2 weeks, and after a rest period for 1 week, the next sample was used.

Adverse reactions
evaluation Observe the presence of skin abnormalities such as erythema, edema, scaling, itching, stinging, burning, stiffness, and prickling at the test site. When an adverse skin reaction occurred, the grade was indicated and the result was written. In addition, a questionnaire survey on adverse skin reactions was conducted with the subjects. Survey A questionnaire survey was conducted on the characteristics of the test subject's general skin condition, skin condition before and after use of the test substance, and feeling of use of the test substance, and one question on the characteristics of the general skin condition and two questions regarding the skin condition before and after use of the test substance were selected. It was investigated using. In addition, in order to examine the feeling of use of the test substance, 4 questions were conducted using the choice of satisfaction/dissatisfaction.

Questionnaire Percentage of subjects (%) Skin type Intelligence 5 Normal (normal skin) 15 Complexity (T-zone intelligence, U-zone intelligence) 55 inattention 25 Sensitiveness 0 Skin condition before test Questionnaire Percentage of subjects (%) My skin is red or not sensitive Not at all 20 Not like that 45 is average 35 Yes 0 it really is 0 The skin is moist and moist Not at all 25 Not like that 30 is average 45 Yes 0 it really is 0

division Percentage (%) after 2 weeks of use skin
More than
reaction
evaluation question answer Example 1 Example 5 Comparative Example 5 Comparative Example 6 The red and sensitive skin feels soothing. Not at all 0 0 5 5 Not like that 0 0 15 5 is average 15 30 15 35 Yes 75 65 65 55 it really is 10 5 0 0 The skin looks moist and moisturized. Not at all 0 0 0 0 Not like that 0 0 0 10 is average 40 35 60 70 Yes 60 65 40 20 it really is 0 0 0 0

Looking at Table 8, in the case of Example 1 and Example 5, it was confirmed that there was no adverse skin reaction. On the other hand, in the case of Comparative Example 5 using an excessive amount of adenosine and Comparative Example 6 using an excessive amount of the compatibilizer, it was confirmed that a somewhat irritating problem was caused to the skin.

Preparation Example 1: Preparation of cream-type cosmetics

In accordance with the preparation method of cream, the raw materials of the contents as shown in Table 9 are used as the oil phase component, and the component soluble in purified water is used as the aqueous phase component, and the raw materials of the above contents are used as a general emulsification method. The water-phase component and the oil phase component were homogeneously mixed with a general homomixer in an emulsification process, neutralized with a carbomer, and then cooled, flavored, and degassed to complete the manufacturing process, thereby producing a cream-type cosmetic product.

Circle Content (% by weight) Cosmetic of Example 1 7.00 glycerin 6.00 Squalane 6.00 Isohexadecane 5.50 Cetyloctanoate 4.00 Glyceryl stearate 2.50 Polysorbite 60 1.00 PEG-100 Stearate 1.00 Stearic acid 1.00 lanolin 1.00 Laureth-12 1.00 Cetyl alcohol 0.50 Sorbitan Sithquioleate 0.30 Carbomer 0.30 Methylparaben 0.20 Imidazolidinyl urea 0.15 Profile Paraben 0.10 Combination perfume 0.10 Purified water The remaining amount in 100% by weight

Preparation Example 2: Preparation of lotion-type cosmetics

In accordance with the preparation method of cream, the raw materials of the contents as shown in Table 10 below are used as the oil phase component, and the component soluble in purified water is used as the aqueous phase component, using the raw materials of the above contents as a general emulsification method. The water-phase component and the oil phase component were homogeneously mixed with a general homomixer in an emulsification process, neutralized with a carbomer, and then cooled, flavored, and degassed to complete the manufacturing process, thereby producing a lotion-type cosmetic product.

Circle Content (% by weight) Cosmetic of Example 1 7.00 Squalane 3.50 Cetyl alcohol 3.00 glycerin 3.00 Glyceryl stearate 2.50 Cetyloctanoate 6.00 Sorbitan Sesquioleate 1.50 PEG-100 Stearate 1.00 Shea Butter 0.50 Carbomer 0.30 Triethanolamine 0.30 Methylparaben 0.20 Imidazolidinyl urea 0.15 Profile Paraben 0.10 Combination perfume 0.10 Purified water The remaining amount in 100% by weight

Preparation Example 3: Preparation of soap

To 91.6% by weight of the purified soap base, 0.4% by weight of alkoxyglycerin, 0.5% by weight of fragrance, 0.5% by weight of moisturizer, and 7% by weight of Example 1 were added, dissolved while stirring at 60°C, cooled to room temperature, and compression molded and cut. Soap was prepared.

In the above, only the preparation of cosmetics and solid soaps in the form of creams and lotions was exemplified, but skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, moisture lotions, nutritional lotions, and Products such as massage cream, nutrition cream, moisture cream, hand cream, sun cream, foundation, essence, nutrition essence, pack, soap, shampoo, conditioner, hair treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser It is natural that it is also possible to manufacture.

As the specific parts of the present invention have been described in detail above, it is clear that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and the scope of the present invention is not limited thereto. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

Contains vitamin C, adenosine, herbal complex extract and solvent,
The herbal complex extract is wormwood extract, green tea leaf extract, peony root extract, centella asiatica leaf extract, Spanish licorice root extract, yam extract, ginseng root extract, mud mushroom extract, golden root extract, sugar elm root extract, raspberry leaf extract , Acne skin improvement cosmetic comprising a mulberry leaf extract, a pine tree leaf extract and a pine leaf extract. The cosmetic composition for improving acne skin according to claim 1, comprising 0.1 to 3.5% by weight of vitamin C, 2.0 to 5.0% by weight of adenosine, 75 to 90% by weight of the herbal complex extract, and the remaining amount of solvent. The method of claim 1, wherein the herbal complex extract comprises 40 to 60 parts by weight of green tea leaf extract, 10 to 40 parts by weight of peony root extract, 50 to 80 parts by weight of Centella asiatica extract, Spanish licorice root 120 to 150 parts by weight of extract, 50 to 150 parts by weight of yam extract, 1 to 10 parts by weight of ginseng root extract, 5 to 20 parts by weight of mud mushroom extract, 30 to 50 parts by weight of golden root extract, 100 to 150 parts of sugar elm root extract An acne skin improvement cosmetic comprising 20 to 50 parts by weight of a raspberry leaf extract, 50 to 100 parts by weight of a mulberry leaf extract, 10 to 40 parts by weight of a pine tree leaf extract, and 20 to 50 parts by weight of a pine leaf extract. The method of claim 2, further comprising 0.1 to 2.0% by weight of the compatibilizer in the total weight,
The compatibilizers are PEG-10 hydrogenated castor oil, PEG-20 hydrogenated castor oil, PEG-25 hydrogenated castor oil, PEG-30 hydrogenated castor oil, PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, PEG-60 hydrogenated An acne skin improvement cosmetic comprising at least one polyoxyethylene hydrogenated castor oil selected from castor oil, PEG-80 hydrogenated castor oil, and PEG-100 hydrogenated castor oil. The cosmetic composition for improving acne skin according to claim 2, further comprising an additive comprising at least one selected from a combination oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a preservative, and a fragrance. Step 1 of preparing the herbal complex extract;
The herbal complex extract, vitamin C, adenosine, and a compatibilizing agent are added to a solvent, followed by stirring to prepare a cosmetic composition.
The herbal complex extract
Based on dry weight, based on 100 parts by weight of wormwood, green tea leaves 40 to 60 parts by weight, peony root 10 to 40 parts by weight, centella leaves 50 to 80 parts by weight, Spanish licorice root 120 to 150 parts by weight, Yakko wheat 50 to 150 Parts by weight, ginseng root 1 to 10 parts by weight, mud mushroom 5 to 20 parts by weight, golden root 30 to 50 parts by weight, sugar elm root 100 to 150 parts by weight, raspberry leaf 20 to 50 parts by weight, mulberry leaf 10 to 30 1-1 step of preparing a dried mixture of 10 to 30 parts by weight of pine needles and 10 to 30 parts by weight of pine needles by weight;
1-2 steps of extracting the dried product by alcohol extraction, hot water extraction, or steam distillation extraction; And
1-3 steps of filtering the obtained extract to obtain a filtrate;
A method for producing a cosmetic for improving acne skin, characterized in that obtained by performing a process comprising a. According to claim 6, If the extract of step 1-2 is an alcohol extract or hot water extract, 1-4 step of obtaining a concentrated herbal composite extract by concentrating the filtrate of step 1-3 under reduced pressure; further comprising A method for producing a cosmetic for improving acne skin, characterized in that. The method of claim 6, wherein the alcohol extraction is performed by immersing the dried mixture in an aqueous ethanol solution of 35 to 50° C. for 12 to 18 hours to obtain an alcohol extract,
In the hot water extraction, the dried mixture is immersed in water and heated at 70 to 90° C. for 2 to 6 hours to obtain a hot water extract,
The steam distillation extraction is a method for producing a cosmetic for improving acne skin, characterized in that to obtain a steam distilled extract in the form of an essential oil obtained by distilling the dried mixture at 80 to 100°C in a bath for 4 to 6 days. The method of claim 6, wherein the mixture in the second step is prepared by further mixing at least one selected from a complex oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a preservative, and a fragrance. It contains the cosmetic of any one selected from claims 1 to 5,
Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, sun cream, foundation, essence, nutrition essence, pack, soap, shampoo, conditioner , Hair treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.