KR100574233B1 - Cosmetic composition comprising plant extract having moisturizing and anti-irritating activity - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing and anti-irritant, and in particular, the present invention relates to a cosmetic composition containing a mixed extract of glutinous barley, barley, soybean and hemp.

The composition of the present invention showed an increased moisturizing power than the control when measuring the moisturizing power by using a desiccator and a corneometer, and showed an anti-inflammatory effect and no irritation when applied to the skin, so the skin's moisturizing and protection was excellent. And it can be used as a cosmetic composition for improvement.

Glutinous barley, pearl barley, soybean, raw horse, moisture, skin improvement, cosmetic

Description

Cosmetic composition containing plant extract having moisturizing and anti-irritant effect

The present invention relates to a cosmetic composition containing an extract obtained from a mixture of glutinous barley, barley, soybean, raw horse, and having a skin moisturizing effect and an anti-irritant effect.

Moisture balance in the skin is controlled by the epidermis. The stratum corneum of the epidermis is the accumulation of dead cells, but plays a very important role in maintaining skin moisture. Human living bodies are mostly composed of water, which plays an important role in maintaining life. The moisture in normal skin is 70% in the body and 20% in the stratum corneum to support the elasticity and flexibility of the skin. If the moisture in the stratum corneum is less than 10%, the skin is known to dry out and cause skin problems. The amount of water in the stratum corneum produced by regular keratinization is always 10-20%. This is due to the sebum film, which is a mixture of sweat and sebum, which protects the skin from the outside and prevents water from evaporating from the stratum corneum. Natural Moisturizing Factor (NMF) and Stratum Corneum Lipid also help to maintain the moisture balance in the stratum corneum. These are present in the stratum corneum to hold and retain moisture. Sebaceous membrane, NMF and dermis are working together to keep skin moisturized. NMF is a hydrophilic hygroscopic substance produced by keratinization process and plays an important role in moisturizing the skin. It is made of amino acids and holds moisture in the horny layer. Also of particular importance are 2-pyrrolidine-5-carboxylic acid (PCA) in NMF. It forms a layer of lipids filled between cells and maintains moisture inside (Seuk Won Won and 2 others, Understanding and Treatment of Skin Aging, Journal of Skin Barriers, 4 , pp71-80, 2002).

Skin is classified into three types: normal, dry and oily. Skin types are classified by the condition of the skin surface, which is determined by the amount of NMF and sebum. If the amount of NMF and sebum is well balanced, the skin stays moist and soft. On the other hand, excessive sebum secretion can lead to oily and acne-prone skin. This type of skin is called oily skin and the pores of the skin come to the surface. On the contrary, if the amount of sebum is too small, the skin cannot keep the skin hydrated. Well-balanced skin is called normal skin and lies between oily and dry skin. In addition, as the skin ages, the amount of sebum secretion decreases, leading to dry skin. This balancing of moisture in the skin involves the water binding capacity of the stratum corneum, the rate of water supply to the inner layer of the stratum corneum, and the rate of water loss on the outer surface of the skin. The stratum corneum of the epidermis is also located on the outermost side of the skin and suppresses the loss of moisture and electrolytes out of the skin, thus preventing the drying of the skin and providing a normal biochemical metabolism of the skin. It also plays an important role in protecting the human body from external physical damage and chemicals or preventing the invasion of bacteria and viruses into the skin (Elias PM . ; J Invest. Dermatol., 80 , pp 44–49, 1983).

Currently, the method of retaining moisture in the skin uses an occlusive agent, which is a hydrophobic substance that promotes water retention by forming a barrier on the skin that prevents water loss. The most commonly used occlusion agents include petrolatum, lanolin, cocoa butter, mineral oil and silicone. In addition, to make cosmetics, 20 to 50 kinds of various raw materials are generally used such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, sodium hydroxide, and the like. Raw materials can be applied to products, which can cause irritation, itching and allergies to the skin.

When the skin is exposed to irritating substances, the stratum corneum is damaged, thus increasing transepidermal water loss, which is an indicator of stratum corneum damage, which may be a primary inflammatory response. Inflammatory reactions that can be caused by the use of cosmetics include irritation such as itching, stinging and burning, erythema and edema, and irritant contact with contact dermatitis. Dermatitis, allergic contact dermatitis, sensory stimulation, phototoxic dermatitis, etc. can be roughly distinguished, but it is difficult to clearly distinguish between these symptoms and often occurs in complex with each other. Above all, in order for the inflammatory reaction to occur, the substance penetrates the skin and the immune system is activated by interacting with epidermis or dermal cells.

 The present invention is to prepare a cosmetic to reduce moisture evaporation on the skin surface as well as to add various nutritional ingredients such as sugar or amino acid to the skin to enhance the moisturizing power of the skin itself and at the same time reduce inflammation or irritation to the skin. .

Waxy barley ( Hordeum vulgare L.) belongs to the family Apothecary, and similar varieties include barley, waxy barley, scented barley and colored barley. Barley contains a large amount of tocotrienol, which inhibits the synthesis of cholesterol. The calcium, fiber, and vitamin B groups are much higher than rice, which makes the skin elastic and contains a large amount of potassium. On the other hand, the cell wall of barley is known to contain a large amount of mixed linked β-D-glucan, in which β (1-3) and β (1-4) glucoside bonds have a certain ratio. In the β-glucan, a water-soluble portion and an insoluble portion coexist, and the water-soluble portion is about 40 to 70%. In general, β- (1,3) (1,4) -D-glucan contains a large amount of hydroxyl groups, which can form a thin moisture moisturizing layer by H-bonding on the skin surface by hydroxyl groups. . In addition to the β-glucan, barley contains a component of tocopherol, which has been reported to be able to play a role in helping the organization's antioxidant defenses as an antioxidant (Per Aman and Hadden Graham; J. Agric. Food. Chem. , 35) . , pp 704-709, 1987).

Yulmu ( Coix lachryma-jobi L.) is also called Haryeom, Okdam, Uiin, and annuals of the rice family are classified as herbaceous. Yulmu contains many ingredients such as 8-20% protein, 2-8% oil, 50-78% carbohydrates and ash. Yulmu rice has a higher protein content than rice or wheat and contains many essential amino acids, including leucine. In addition, it is rich in oil and trace elements. Yulmu is known to have anti-inflammatory and lower cholesterol content. In oriental medicine, swelling and swelling are used as inflammatory drugs, diuretics, pear pesticides, and analgesics, which are used for neuralgia and arthralgia. , Ilwol Seokji, pp762 ~ 764, 1999).

Soybean ( Glycine max (L.) Merrill) is a botanical plant belonging to the species Legumes (Leguminosae), Papilionoideae and Glycine L. The seeds contain 36-45% protein and 17 oils. 22%, in addition to triterpenoids saponin, nitrogen compounds, carbohydrates, candy, pectin, minerals and vitamins. 80-90% of the protein consists of essential amino acids such as arginine, methionine, threonine, leucine, isoleucine and valine. The phospholipid content of seeds contains lecithin, kephalin, etc., and nitrogen compounds contain adenine, guanine, choline, trigonelin, and histidine, an amino acid (interest, ingredient and use of herbal medicine, p. 187, 1999). ).

Raw horses are generally known as hemps or medicinal herbs and have the scientific name of hemp ( Dioscorea batatas Decaisne) or yam ( Dioscorea japonica Thunb). It contains a large amount of starch and a glycoprotein, mucin (0.5%), which is hydrolyzed to produce sugars such as mannose and glycine and 16 amino acids. Others include allantoin, arginine, diastase, and masaponin (0.03% to 0.07%). Since ancient times, it has been used as a remedy for sickness recovery, general weakness, weakness, fatigue recovery (Information, Shin Min-kyo, Painted medicinal fragrance dictionary, Younglimsa, pp148-149, 1990).

Korean Patent Laid-Open Publication No. 2003-0039389 discloses a cosmetic composition for cosmetic packs and a method of manufacturing the same having a very excellent effect on maintaining the elasticity of the skin and preventing aging, including various herbal ingredients extracts and natural extracts in addition to Yulmu, Publication No. 60-214721 discloses a cosmetic composition for whitening having tyrosinase inhibitory activity, which contains plant extracts such as bark skin, peony root, geranium, sesame seeds, and yulmu, and Korean Patent No. 337113 discloses soybean extract 0.01-5.0. Disclosed is a skin elasticity-improving cosmetic comprising: 0.01% to 5.0% by weight and / or malt extract and 0.01% to 5.0% by weight wheat protein. In addition, Korean Patent Publication No. 2003-25716 discloses a whitening cosmetic which adds an organic solvent-extracted extract to a conventional basic cosmetic by removing the bark of the plant root stem belonging to the hemp and the yam family, and then crushing and drying the dried bark.

Therefore, the present inventors have conducted research on natural products having excellent moisturizing and anti-irritant effects, and as a result, the composition containing the mixed extract of the barley, barley, soybean, and hemp is effective in enhancing skin moisturizing, anti-irritating, and anti-inflammatory effects. The present invention has been found to be very useful for skin improvement.

Therefore, the present invention contains extracts of natural plants, not only inhibits evaporation of water on the surface of the skin, but also adds various nutrients such as sugars and amino acids to the skin to enhance the moisturizing power of the skin itself and at the same time, To make cosmetics to reduce irritation.

In order to achieve the above object, the present invention provides a cosmetic composition containing a mixed extract of the barley ( Hordeum vulgare L.), Yulmu ( Coix lachryma-jobi L.), soybean ( Glycine max (L.) Merrill) and raw horse to provide.

The glutinous barley, yulmu, soybeans and raw horses are dry weight ratio of 1 to 50: 1 to 30: 1 to 20: 1 to 30, preferably 1 to 5: 1 to 3: 1 to 2: 1 to 3, more preferably Preferably, 2: 1: 1: 1 can be used for the preparation of extracts, and each extract may have a dry weight ratio of 1 to 50: 1 to 30: 1 to 20: 1 to 30, preferably 1 to 5: 1 to 3: 1 to 2: 1 to 3, more preferably 2: 1: 1: 1 can be used by mixing.

The raw horses include hemp ( Dioscorea Batatas Decaisne ) or yam ( Dioscorea japonica Thunb.).

The mixed extract is characterized in that it contains 0.001 to 20% by weight, preferably 0.01 to 10% by weight relative to the total weight of the composition.

Hereinafter, the present invention will be described in detail.

The mixed extract of the present invention, after completely drying the glutinous barley, barley, soybeans and raw horses, finely chopped or pulverized, in each of the pulverized or 1 to 50%, barley 1-30%, soybeans 1-20%, soybeans To the mixture mixed at a dry weight ratio of 1 to 30%, about 1 to 30 times the weight (kg), preferably about 5 to 15 times the water, C ₁ to C ₄ lower alcohol, butylene glycol, propylene Glycol, glycerin or a mixed solvent thereof, preferably distilled water is added, or in addition thereto, the acidity is adjusted to a range of pH 8-11 or pH 3-6 with aqueous sodium carbonate solution or diluted hydrochloric acid, and then 0 ° C. to 100 ° C. Extraction may be performed by using cold soaking, hot water extraction, ultrasonic extraction, reflux cooling extraction for about 0.5 hours to 15 days at an extraction temperature, and the extract thus obtained may be filtered, concentrated under reduced pressure, or dried. At this time, the extract extracted under acidic or alkaline conditions is adjusted to a neutral pH, preferably 7.0 to 7.5 before filtration, and then filtered, concentrated under reduced pressure or dried.

In the above preferred extraction method, the cooling condenser is installed in the state of preventing the solvent from evaporating 37 ~ 95 ℃, extracted by heating for 0.5 to 20 hours or by dipping at 0 ~ 37 ℃ for 1 to 15 days to extract the active ingredient The method may be used, and the plant mixture extract thus extracted may be used by filtration or concentrated under reduced pressure and then powdered while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser.

The mixed extract of the present invention prepared by the above is preferably an extract containing a certain solid content, when the moisturizing power measurement using a desiccator and corneometer, showed an increased moisturizing power than the control, anti-inflammatory test and skin primary Through irritation test (closed patch test) it was confirmed that there is no irritation without causing inflammation on the skin, and when applied to the skin, the skin improvement effect is excellent, and it can be used as a cosmetic composition for moisturizing, protecting and improving the skin.

The present invention contains a mixed extract of glutinous barley, barley, soybeans, and raw horses in an amount of 0.001 to 20% by weight, preferably 0.01 to 10% by weight of the total cosmetic composition and is applied cosmetically according to a conventional method in the field of cosmetic preparation The moisturizing cosmetic composition which mixed the possible base material is provided.

In addition, the cosmetic composition of the present invention can be applied and blended as necessary components, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., which are formulated in general skin cosmetics. Do.

The cosmetic composition containing the mixed extract of the present invention as an active ingredient may be used in a variety of moisturizing cosmetics, face wash, body cosmetics and the like.

Examples of products to which the present composition can be added include cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and cleansing agents, face washes, soaps, treatments, and essences. .

Specific examples of the cosmetic of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, essence, nutrition essence, pack, soap, Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex and the like.

The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Examples 1-9. Warming extract manufacturer

200 g of fully dried glutinous barley, 100 g of barley radish, 100 g of soybean and 100 g of raw yam were mixed and pulverized to make 500 g of mixed raw materials. Then, 5 kg of each extraction solvent was added and heated and extracted at 50 ° C. for 3 hours in an extractor equipped with a cooling condenser. It was prepared by filtration with filter cloth and final filtration with 0.45 μm filter. The results are shown in Table 1.

Extraction solvent Solid content (%) Yield (kg) Example 1 Distilled water 1.92 4.23 Example 2 Ethyl alcohol 1.46 4.47 Example 3 30% hydrous ethyl alcohol 1.75 4.38 Example 4 Butylene glycol 1.53 4.15 Example 5 30% water butylene glycol 1.75 4.35 Example 6 Propylene glycol 1.42 4.12 Example 7 30% water propylene glycol 1.85 4.26 Example 8 glycerin 1.49 4.05 Example 9 30% water glycerine 1.61 4.27

Examples 10-17. Room temperature extraction preparation

200 g of completely dried glutinous barley, 100 g of barley radish, 100 g of soybean and 100 g of raw yam were mixed and ground to make 500 g of mixed raw materials. 5 kg of each extraction solvent was added thereto, and aged at room temperature for 7 days, followed by filtration with 400 mesh filter cloth and 0.45 μm filter. Prepared by final filtration. The results are shown in Table 2 below.

menstruum Solid content (%) Yield (kg) Example 10 Ethyl alcohol 1.03 4.37 Example 11 30% hydrous ethyl alcohol 1.32 4.22 Example 12 Butylene glycol 1.02 4.00 Example 13 30% water butylene glycol 1.40 4.35 Example 14 Propylene glycol 1.15 4.25 Example 15 30% water propylene glycol 1.30 4.32 Example 16 glycerin 1.02 3.97 Example 17 30% water glycerine 1.44 4.06

Examples 18-20. Extraction Preparation with Alkaline Solution

200 g of fully dried barley barley, 100 g of barley radish, 100 g of soybean and 100 g of raw yam are mixed and ground to make 500 g of mixed raw materials, and 5 kg of each extraction solvent is added thereto, and then adjusted to pH 9-10 with 10% aqueous sodium carbonate solution and equipped with a cooling condenser. The extract was warmed at 50 ° C. for 3 hours, filtered through a 400 mesh filter cloth, and adjusted to pH 7 with 1% dilute hydrochloric acid. This extract was prepared by final filtration with a 0.45 μm filter. The results are shown in Table 3.

Extraction solvent Solid content (%) Yield (kg) Example 18 Distilled water 1.08 4.39 Example 19 30% hydrous ethyl alcohol 0.88 4.18 Example 20 30% water butylene glycol 0.82 4.18

Examples 21-23. Preparation of Extraction by Acidic Solution

200 g of fully dried glutinous barley, 100 g of barley radish, 100 g of soybean and 100 g of raw yam are mixed and ground to make 500 g of mixed raw material. 5 kg of each extraction solvent is added thereto, and the pH is adjusted to 3-4 with 1% diluted hydrochloric acid and The extract was warmed at 50 ° C. for 3 hours, filtered through a 400 mesh filter cloth, and adjusted to pH 7 with an aqueous 10% sodium carbonate solution. This extract was prepared by final filtration with a 0.45 μm filter. The results are shown in Table 4.

Extraction solvent Solid content (%) Yield (kg) Example 21 Distilled water 1.14 4.28 Example 22 30% hydrous ethyl alcohol 0.89 4.11 Example 23 30% water butylene glycol 0.83 4.21

Experimental Example 1. Measurement of moisturizing power using desiccator

The moisturizing effect of the extract was measured using the difference in the rate of water evaporation inhibition in the liquid as follows, and each extraction solvent was used as a control. In addition, solvents containing no water were excluded from the experiment.

After 5 ml of the sample was titrated into a cup having a constant surface area, it was left for 24 hours in a desiccator adjusted to a temperature of 35 ° C. using completely dried calcium chloride (CaCl ₂ ) as an absorbent, and each sample was weighed 5 times after 24 hours. Moisturizing power was measured using the measured average value. The results are shown in Table 5.

Extraction solvent Moisturizing power (%) Moisturizing rate increase (times) Experiment Control Example 1 Distilled water 43.2 29.8 1.45 Example 3 30% hydrous ethyl alcohol 32.3 26.7 1.21 Example 5 30% water butylene glycol 48.7 42.6 1.14 Example 7 30% water propylene glycol 49.3 43.3 1.14 Example 9 30% water glycerine 52.5 47.2 1.11 Example 11 30% hydrous ethyl alcohol 32.4 26.7 1.21 Example 13 30% water butylene glycol 47.6 42.6 1.12 Example 15 30% water propylene glycol 48.9 43.3 1.13 Example 17 30% water glycerine 54.4 47.2 1.15 Example 18 Distilled water 39.4 29.8 1.32 Example 19 30% hydrous ethyl alcohol 29.2 26.7 1.09 Example 20 30% water butylene glycol 46.2 42.6 1.08 Example 21 Distilled water 38.8 29.8 1.30 Example 22 30% hydrous ethyl alcohol 29.7 26.7 1.11 Example 23 30% water butylene glycol 45.5 42.6 1.07 -Calculation method ① Moisturizing power (%) = (1-ΔS / S) × 100 S: Initial sample weight ΔS: Initial sample weight-Final sample weight ② Moisturizing power increase rate (times) = Experimental moisturizing power / control moisturizing power

Experimental Example 2 Measurement of Moisturizing Power Using Corneometer CM 825

The moisturizing effect of the extract was measured using Corneo CM CM 825 (Corneometer CM 825, Courage + Khazaka Co.), and each extraction solvent was used as a control.

Ten women in their 20s for each sample were divided into 8 x 2 cm 2 upper arms and 8 compartments, washed several times with water, and then removed with no irritation. After 15 minutes, the samples were applied evenly by dropping 10 µl into each of the two sections. After 2 hours, 5 measurements were taken per compartment using a corneometer. The experiment was conducted in a constant temperature and humidity room with a room temperature of 21 ± 2 ℃ and a relative humidity of 50 ± 5%. The measured value was used by averaging the value obtained by measuring five times, and is shown in Table 6 using the average value of five subjects.

Extraction solvent Moisturizing Increased moisture Experiment Control Example 1 Distilled water 15.44 1.63 13.81 Example 2 Ethyl alcohol 3.72 0.04 3.68 Example 3 30% hydrous ethyl alcohol 8.63 0.02 8.61 Example 4 Butylene glycol 46.13 39.58 6.55 Example 5 30% water butylene glycol 25.08 19.92 5.16 Example 6 Propylene glycol 50.66 41.90 8.76 Example 7 30% water propylene glycol 25.50 22.50 3.00 Example 8 glycerin 66.54 52.22 14.32 Example 9 30% water glycerine 52.30 46.43 5.87 Example 10 Ethyl alcohol 2.36 0.04 2.32 Example 11 30% hydrous ethyl alcohol 6.68 0.02 6.66 Example 12 Butylene glycol 46.02 39.58 6.44 Example 13 30% water butylene glycol 27.39 19.92 7.47 Example 14 Propylene glycol 55.62 41.90 13.72 Example 15 30% water propylene glycol 28.33 22.50 5.83 Example 16 glycerin 65.82 52.22 13.60 Example 17 30% water glycerine 50.38 46.43 3.95 Example 18 Distilled water 8.92 1.63 7.29 Example 19 30% hydrous ethyl alcohol 4.22 0.02 4.20 Example 20 30% water butylene glycol 25.30 19.92 5.38 Example 21 Distilled water 12.39 1.63 10.76 Example 22 30% hydrous ethyl alcohol 7.02 0.02 7.00 Example 23 30% water butylene glycol 25.66 19.92 5.74 -Calculation method (Corneometer Value) = S-ΔS S: Corneometer Value before sample application, ΔS: Corneometer Value after 2 hours of sample application ② Moisture increase = Experimental moisturizing power-Control moisturizing power

Experimental Example 3. Anti-inflammatory Test

The anti-inflammatory effect of the extract was measured to induce inflammation and inhibition by Arachidonic acid (Arachidonic acid) using a mouse.

Using the left ear of the mouse as the control site and the right ear as the test site, the ears were washed with ethanol before application of the sample, and 20 μl of the samples of Examples 1 to 23 were continuously applied once a day for 4 days, and the last application for 1 hour. Thereafter, 2 mg / ear of ethanol was applied to the left ear, and arachidonic acid was applied to the right ear. After 1 hour, the degree of ear edema was repeatedly measured three times on both ears with a micrometer.

The anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group. Indomethacin was treated in the positive control group of the anti-edema effect, and the results are shown in Table 7.

Ear thickness and inflammation inhibition rate by topical application sample density(%) menstruum Ear thickness (㎛) % Inhibition Arachidon acid non-treated ears Arachidonic acid treated ears Arachidonic acid 2mg / ear ethanol 266 551 - Indomethacin One ethanol 243 379 52.3 Example 1 Extract 5 ethanol 228 458 19.3 Example 2 Extract 5 ethanol 253 498 14.1 Example 3 Extract 5 ethanol 272 523 11.8 Example 4 Extract 5 ethanol 283 532 12.5 Example 5 Extract 5 ethanol 272 514 15.2 Example 6 Extract 5 ethanol 280 528 13.1 Example 7 Extract 5 ethanol 280 534 11 Example 8 Extract 5 ethanol 276 528 11.5 Example 9 Extract 5 ethanol 266 519 11.3 Example 10 Extract 5 ethanol 259 514 10.6 Example 11 Extract 5 ethanol 257 511 10.8 Example 12 Extract 5 ethanol 260 511 11.9 Example 13 Extract 5 ethanol 272 513 15.5 Example 14 Extract 5 ethanol 270 513 14.6 Example 15 Extract 5 ethanol 271 512 15.5 Example 16 Extract 5 ethanol 253 493 15.7 Example 17 Extract 5 ethanol 251 487 17.1 Example 18 Extract 5 ethanol 255 501 13.8 Example 19 Extract 5 ethanol 273 514 15.6 Example 20 Extract 5 ethanol 277 515 16.5 Example 21 Extract 5 ethanol 268 519 11.8 Example 22 Extract 5 ethanol 250 497 13.5 Example 23 Extract 5 ethanol 272 510 16.5 ※ Inhibition rate (%) = (A-B) / A × 100 A: Average thickness of control ears (thickness of arachidonic acid-treated ears-thickness of untreated ears) B: Thickness of sample-coated group ears (thickness of arachidonic acid-treated ears-untreated ears thickness)

Experimental Example 4. Skin primary irritation test (closed cloth test)

For the extract, the effect of stimulating the stimulation was measured by the inhibition of the stimulation caused by sodium lauryl sulfate using a closed patch test.

Twenty subjects were wiped with 70% ethanol and dried. An experimental solution was prepared in 5% of the samples of Examples 1 to 23 and 0.08% of sodium lauryl sulfate in purified water, and 20 µl of the experimental solution was added dropwise to a Finn chamber. A paper disc was placed on each pin chamber, and the pin chamber was fixed on the test site. After patching for 24 hours, the test site was marked with a marking pen to determine the result after 4 hours. Judgment was classified according to the following criteria for stimulation, the results are shown in Table 8.

sample Number of test subjects Judgment result Stimulus ++ + +- - Sodium Lauryl Sulfate 0.08% 20 One 2 8 9 0.75 Purified water 20 0 0 3 17 0.15 Example 1 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 2 18 0.10 Example 2 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 One 2 17 0.20 Example 3 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 One 5 14 0.35 Example 4 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 3 17 0.15 Example 5 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 One 4 15 0.30 Example 6 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 5 15 0.25 Example 7 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 0 6 14 0.30 Example 8 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 0 2 18 0.10 Example 9 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 One 0 3 16 0.30 Example 10 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 2 5 13 0.45 Example 11 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 0 6 14 0.30 Example 12 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 0 2 18 0.10 Example 13 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 One 19 0.05 Example 14 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 3 3 14 0.45 Example 15 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 One 0 5 14 0.40 Example 16 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 2 6 12 0.50 Example 17 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 0 7 13 0.35 Example 18 Extract 5.0% + Sodium Lauryl Sulfate 0.08% 20 0 One 8 11 0.50 Example 19 5.0% Extract + Sodium Lauryl Sulfate 0.08% 20 0 0 9 11 0.45 Example 20 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 0 8 12 0.40 Example 21 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 2 8 10 0.60 Example 22 extract 5.0% + sodium lauryl sulfate 0.08% 20 0 0 7 13 0.35 Example 23 extract 5.0% + sodium lauryl sulfate 0.09% 20 0 0 6 14 0.30

Criteria - ; No erythema or unusual phenomena +-; Slightly redder than +; Markedly redder than ++; Severe redness and swelling than the surroundings

Preparation Example 1 Preparation of Lotion

An example of preparation of a lotion (skin, lotion) in a cosmetic containing an extract obtained from a mixture of glutinous barley, barley, soybean, and raw horse is as follows. Here the plant mixture extract is that of Example 1.

number Raw material Formulation example (% by weight) Comparative formulation example (% by weight) One Plant mix extract 5.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene (60) Cured Castor Oil 1.00 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 1 2, 3 and 4 were added sequentially to No. 11 to be stirred to dissolve. Then, No. 5 was heated and dissolved at about 50 ° C., and 6, 8 and 10 were added to No. 11 after stirring. Finally, 1, 7, and 9 are added and sufficiently stirred before aging.

Comparative Formulation Example 1 No. 2, 3, and 4 were added sequentially to No. 11 to be dissolved by stirring. Then, No. 5 was heated to about 50 ° C. to dissolve. . Finally, add 7, 9, and stir sufficiently to mature.

Preparation Example 2 Preparation of Nutrients

An example of preparation of nutrient lotion in cosmetics containing an extract obtained from a mixture of glutinous barley, barley, soybean, and raw horse is as follows. Here the plant mixture extract is that of Example 1.

number Raw material Formulation example (% by weight) Comparative formulation example (% by weight) One Plant mix extract 5.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 10.0 10.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.3 0.3 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 2: Raw materials 2, 3, 4, 5 and 6, 7, and 9 are heated to 80-85 ° C. with mixing and stirring, and then put into the manufacturing unit, the emulsifier is operated, and raw materials 8, 13, and 15 are 80 It is emulsified after heating to ˜85 ℃. 10, 11 and 12 were added and mixed, and the mixture was cooled to 40 ° C. while stirring using a stirrer, and then 1, 14 were added and cooled to 30 ° C. before aging.

Comparative Formulation Example 2 Raw Materials 2, 3, 4, 5 and 6, 7, and 9 were mixed and stirred and heated to 80 to 85 ° C., and then put into the manufacturing unit, and then the emulsifier was operated and raw materials 8, 13 and 15 were replaced. The mixture is heated to 80 ~ 85 ℃ and then emulsified. Then, 10, 11 and 12 are added and mixed. The mixture is cooled to 40 ℃ with stirring using a stirrer, then 14 times is added and cooled to 30 ℃ and then aged. Let's do it.

Preparation Example 3 Preparation of Nutritional Cream

An example of preparation of nutritional cream in cosmetics containing extracts obtained from a mixture of glutinous barley, barley, soybean, and raw horse is as follows. Here the plant mixture extract is that of Example 1.

number Raw material Formulation example (% by weight) Comparative formulation example (% by weight) One Plant mix extract 5.0 - 2 Cytostolol 4.0 4.0 3 Polyglyceryl 2-oleate 3.0 3.0 4 Ceramide 0.7 0.7 5 Ceteares-4 2.0 2.0 6 cholesterol 3.0 3.0 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 22.0 22.0 10 Carboxy Vinyl Polymer 0.5 0.5 11 Triethanolamine 0.5 0.5 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 3: Raw materials 2, 3, 4, 5 and 6, 7, and 9 are mixed and stirred and heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier is operated. It is emulsified after heating to 85 ℃. After mixing 10 and 11 times, the mixture was cooled to 40 ° C. while stirring using a stirrer, and 1, 13 times were added thereto, cooled to 30 ° C., and aged.

Comparative Formulation Example 3 The raw materials 2, 3, 4, 5 and 6, 7, and 9 were mixed and stirred to be heated to 80-85 ° C. and mixed into the manufacturing unit, and then the emulsifier was operated. Emulsify after heating to 80 ~ 85 ℃. After mixing 10 and 11 times, the mixture was cooled to 40 ° C. while stirring using a stirrer, and then 13 times were added, cooled to 30 ° C., and aged.

Preparation Example 4 Preparation of Massage Cream

An example of preparation of a massage cream in a cosmetic composition containing an extract obtained from a mixture of glutinous barley, barley, soybean, and raw horse is as follows. Here the plant mixture extract is that of Example 1.

number Raw material Formulation example (% by weight) Comparative formulation example (% by weight) One Plant mix extract 5.0 - 2 Beeswax 10.0 10.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.8 0.8 5 Liquid paraffin 40.0 40.0 6 Squalane 5.0 5.0 7 Caprylic / Capric Triglycerides 4.0 4.0 8 Carboxy Vinyl Polymer 0.2 0.2 9 glycerin 5.0 5.0 10 Butylene glycol 3.0 3.0 11 Propylene glycol 3.0 3.0 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 4: 8, 9, 10, 11, 13, 16 and stirred at 80 ~ 85 ℃ while stirring the mixture into the manufacturing unit after the emulsifier is operated and 2, 3, 4, 5, 6, 7, 12 The bun is heated to 80 ~ 85 ℃ and then emulsified. After emulsification, cool to 50 ℃ while stirring using a stirrer, add 14 times, cool to 40 ℃, add 1, 15 times, cool to 30 ℃ and mature.

Comparative Formulation Example 4 After mixing 8, 9, 10, 11, 13, and 16, the mixture was heated to 80 ° C to 85 ° C, put into the manufacturing unit, and the emulsifier was operated. 2, 3, 4, 5, 6, 7, 12 is heated to 80 ~ 85 ℃, and then emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then 14 times added, cooled to 40 ° C., 15 times added, cooled to 30 ° C. and aged.

Preparation Example 5 Preparation of Nutritional Essence

An example of preparation of a nutritional essence in a cosmetic composition containing an extract obtained from a mixture of glutinous barley, barley, soybean, and raw horse is as follows. Here the plant mixture extract is that of Example 1.

number Raw material Formulation example (% by weight) Comparative formulation example (% by weight) One Plant mix extract 5.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 15.0 15.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.2 0.2 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 5: Raw materials 2, 3, 4, 5 and 6, 7, and 9 are mixed and stirred to be heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier is operated. It is emulsified after heating to 85 ℃. 10, 11 and 12 were added and mixed, and then cooled to 40 ° C. while stirring using a stirrer, and then 1, 14 were added, cooled to 30 ° C., and aged.

Comparative Formulation Example 5 The raw materials 2, 3, 4, 5 and 6, 7, and 9 were mixed and stirred to be heated to 80-85 ° C. with mixing and stirring, and then the emulsifier was operated and the raw materials 8, 13 and 15 were 80. It is emulsified after heating to ˜85 ℃. 10, 11 and 12 were added and mixed, and then cooled to 40 ° C. while stirring using a stirrer, and then 14 times were added and cooled to 30 ° C. before aging.

Preparation Example 6 Preparation of Body Cleanser

An example of preparation of a body cleanser in a cosmetic containing an extract obtained from a mixture of glutinous barley, barley, soybean, and raw horse is as follows. Here the plant mixture extract is that of Example 1.

number Raw material Formulation example (% by weight) Comparative formulation example (% by weight) One  Plant mix extract 5% - 2 Sodium Lauryl Sulfate (30% aqueous solution) 15.0 15.0 3 Sodium lauryl ether sulfate (30% aqueous solution) 11.0 11.0 4 Coconut Diethanol Amide 4.0 4.0 5 Myristic acid 2.0 2.0 6 Lauric acid 2.0 2.0 7 glycerin 3.0 3.0 8 Triethanolamine 2.0 2.0 9 antiseptic Quantity Quantity 10 Softener Quantity Quantity 11 Pigment Quantity Quantity 12 Spices Quantity Quantity 13 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 6 After heating raw materials 4, 5, 6, and 7 to 70 ° C., mixing raw materials 2, 3, 8, 9, 10, 11, 13, heated to 70 ° C., injected into a tank, and stirred and mixed Subsequently, the raw materials 1 and 12 are gradually added and homogenized at 40 ° C., cooled to 30 ° C., stabilized, and aged.

Comparative Formulation Example 6 After heating raw materials 4, 5, 6 and 7 to 70 ° C, mixed raw materials 2, 3, 8, 9, 10, 11 and 13, heated to 70 ° C, injected into tank and stirred After mixing, the raw material No. 12 is gradually added and homogenized at 40 ° C., cooled to 30 ° C., stabilized, and aged.

Experimental Example 1. Confirmation test for improving skin condition

Formulation Example 3 and Comparative Formulation Example 3 When the skin was applied to the nourishing cream, the following clinical confirmation experiment was conducted to confirm the skin condition improvement effect. Ten women aged 20 to 55 years were examined for skin condition improvement and skin oil and moisture.

To the same person, the nutritional cream of Formulation Example 3 was applied to the upper part of the face, and the nutritional cream of Comparative Formulation Example 3 was applied to the skin once a day for 20 days once a day after washing the face every morning. The degree of improvement was measured. The test results are shown in Table 15.

                         Survey Items Skin condition improvement Number of people % Formulation Example 3 very good 4 20 good 8 40 is average 6 30 so so 2 10 Comparative Formulation Example 3 very good 2 10 good 6 30 is average 10 50 so so 2 10

It can be seen that the skin improvement effect is superior to Comparative Formulation Example 3 prepared without addition of the cosmetic formulation of Formulation Example 3 prepared by adding the plant mixture extract as described above.

The cosmetic composition containing the mixed extract of the barley, barley, soybean and hemp of the present invention not only relieves irritation to the skin but also inhibits inflammation and has excellent moisturizing power, and thus can be used as an anti-irritant cosmetic for moisturizing.

Claims (7)

Moisturizing anti-irritant cosmetic composition containing a mixed extract of Hordeum vulgare L., Coix lachryma-jobi L., Glycine max (L.) Merrill and raw horse. delete The cosmetic composition according to claim 2, wherein the mixing ratio of the glutinous barley: silk radish: soybean: draft is 1-5: 1-3: 1-2: 1-3. The cosmetic composition according to claim 1, wherein the extract is extracted using water, C ₁ to C ₄ lower alcohols, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof as an extraction solvent. The cosmetic composition according to claim 1, wherein the extract is extracted by adding an extraction solvent to the mixture and then adjusting the acidity to a range of pH 8-11 or pH 3-6. The moisturizing anti-irritant cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition. The composition of claim 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap Cosmetic composition, characterized in that it has any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.