KR101154772B1 - Cosmetic composition containing the zizyphus jujuba fruit extract and walnut extract for moisturizing effect on the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition containing jujube extract and walnut extract. More particularly, the present invention relates to a skin moisturizing cosmetic composition containing jujube extract and walnut extract. The jujube extract of the present invention promotes keratin differentiation among herbal medicines mainly used in herbal medicine, and the walnut extract contains rich lipids. It can retain moisture in the stratum corneum. The cosmetic composition containing the jujube extract and the walnut extract of the present invention promotes the differentiation of the stratum corneum by keratinocytes in the cells, restores the barrier function of the damaged skin, and improves and maintains the skin's moisturizing power even in human experiments. Since it shows, it can be usefully used as a cosmetic composition for skin moisturizing.

Jujube, Hodo, extract, herbal medicine, keratin, differentiation, barrier function, skin, moisture, cosmetics, composition.

Description

Cosmetic composition for skin moisturizing containing jujube extract and walnut extract {molecular cosmetic composition containing the zizyphus jujuba fruit extract and walnut extract for moisturizing effect on the skin}

The present invention relates to a cosmetic composition containing jujube extract and walnut extract.

The skin, which plays an important role as a barrier to protect the living body from the external environment, develops into a thin stratum corneum while containing moisture through differentiation process in the epidermal layer to prevent drying of moisture. The stratum corneum is the skin's outermost layer that is in direct contact with the external environment, preventing the invasion of mechanically and chemically harmful ingredients, preventing internal moisture from being lost in the drier external environment, and the stratum corneum also retains adequate moisture for flexibility. Is keeping.

Skin moisture level is about 70% in the dermis, but decreases to the epidermis, about 10-30% in the outermost stratum corneum. Proper moisture is maintained in the stratum corneum to maintain the elasticity and suppleness of the skin. Normally, normal skin contains about 30% water or water-soluble components.

In addition, keratinocytes in healthy humans have high concentrations of Natural Mosituring Factors (NMF) to help maintain skin moisture.

However, despite this function of the skin, the skin is exposed to the harsh external environment, such as wind, cold weather, sunlight, washing, shaving, and winter heating in the contaminated atmosphere, and the aging factor increases with age. The skin easily loses moisture and is easy to dry, so there is an increasing need for skin moisturizers to prevent this.

Therefore, there are a number of substances for the purpose of moisturizing, and many studies have been conducted on this. Until now, known skin moisturizers increase the water retention in the stratum corneum such as lipid components such as ceramide and essential fatty acids and lipid complexes. It is common to use substances that can be used ( J. Invest. Dermatol. , 5 , pp 731-740, 1994).

Recently, it has been newly discovered that not only increases water in the stratum corneum but also differentiates from the basal keratinocytes to the outermost stratum corneum cells, which can act as a barrier for retaining skin moisture. lost. In other words, physiologically, skin drying is a function of maintaining skin moisture by promoting the differentiation of keratinocytes because the stratum corneum's dropout time is prolonged or the lipid synthesis ability of the epidermal cells decreases and the amount of moisturizing factors and lipids in the skin stratum corneum is reduced. Can promote Therefore, it can be expected that even more excellent skin moisturizing effect can be obtained by using a water-containing substance in the stratum corneum and a substance that serves to promote keratin differentiation.

In addition, recently, with the increasing interest in herbal medicine, researches are actively being conducted to obtain useful ingredients for human body from natural herbal medicines that are safe for human body.

Therefore, the inventors of the present invention while searching for ingredients with a moisturizing effect of herbal medicines according to such a reality, walnuts as a component that can supply jujube extract and lipids as ingredients that can promote the skin keratin differentiation among these herbal ingredients The extract was found, and the two extracts were used together to prepare a cosmetic and apply it to human skin. As a result, it was confirmed that the excellent keratin differentiation and skin moisturizing effect was completed, thereby completing the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing jujube extract and walnut extract, and more specifically, to contain both jujube extract and walnut extract together to promote better keratinization and moisturize the skin than when each component is used alone. It is to provide a cosmetic composition for skin moisturizing effect.

In order to achieve the above object, the present invention provides a cosmetic composition containing the jujube extract and walnut extract. More specifically, it provides a skin moisturizing cosmetic composition containing jujube extract and walnut extract.

The jujube extract is characterized in that it is used as a substance to promote the keratin differentiation, the walnut extract is characterized by containing abundant lipids to retain the moisture of the stratum corneum.

The jujube and walnut are characterized by using the fruit of the jujube and walnut tree, respectively.

In addition, the cosmetic composition for moisturizing the skin preferably contains a jujube extract in a concentration of 0.001 to 20% by weight of the total composition. This is because when the content of the jujube extract is less than 0.001% by weight, there is no skin moisturizing effect, and when the content of the jujube is more than 20% by weight, a problem occurs in skin safety.

The skin moisturizing cosmetic composition preferably contains a walnut extract in a concentration of 0.001 to 20% by weight of the total composition. This is because when the content of the walnut extract is 0.001% by weight or less, there is no skin moisturizing effect, and when the content of the walnut extract is 20% by weight or more, a problem occurs in skin safety.

In addition, the composition ratio of the jujube extract and the walnut extract in the cosmetic composition of the present invention is characterized in that it is contained in a weight ratio of 1: 9 to 9: 1. If either one of the jujube extract and the walnut extract in the total cosmetic composition is too small compared to the other one, the synergistic effect of the moisturizing effect due to the two functions of promoting keratinocyte differentiation and water retention of the stratum corneum, jujube extract and walnut The weight ratio of the extract should be at least 1: 9 to 9: 1.

Hereinafter, the present invention will be described in more detail.

Jujube used in the present invention refers to the fruit of the jujube tree ( Zizyphus jujuba ), also called crude (목) or wood wheat (木 (). The surface is reddish brown, elliptical, 1.5 ~ 2.5 cm long, and ripe when red. Jujube fruit has 20 ~ 49% of dry matter, and 14 ~ 42% is composed of sugars such as fructose, sucrose, glucose and rhamnose. In addition, organic acids such as malic acid, grape acid, succinic acid, and glycolic acid contain 0.54-3.4%, and are rich in minerals such as potassium, calcium and magnesium. Fruits are not only reproduced, but also processed in life and used as jujube, jujube tea, jujube vinegar and jujube. In oriental medicine, it is used as a diuretic, tonic, and emollient. In addition, in oriental medicine, the muscles are aching disease, when the muscles are painful, cough, when the whole body hurts are mainly used and is known to be effective in exfoliating skin with anti-allergic effect.

The walnut used in the present invention is a fruit of the walnut tree ( Juglans sinensis ), which is a deciduous tree of the sputum family, and is almost circular and has a nucleus. It is native to Europe but is distributed in China and Asia and is cultivated all over the world. Korea is mainly distributed south of central part. The chemical composition of walnuts is especially higher in protein than in meat and twice as much in lipid as pork. In addition, the lipid contains a lot of unsaturated fatty acids and essential fatty acids that lower serum cholesterol. It is known to give. In addition, if the minerals and vitamins B ₁ to eat every rich becomes skin glazing Kowakien, and even anti-aging and tonic effect, according to the Compendium of Materia Medica walnut heal the beam, and haejugo warm waist and knee constipation liver and get rid of phlegm It is used to strengthen kidney function, enhance memory, and treat nervous breakdown. In general, it is known that lipid retention can be used to increase the water retention of the stratum corneum, so walnuts rich in lipids are also thought to prevent skin drying and make the skin hydrated.

Jujube extract and walnut extract of the present invention can be obtained by the following method. First, jujube extract is extracted by boiling water for about 30 minutes to 12 hours under a reflux apparatus after adding a certain amount of water to the jujube, filtered and the filtrate is taken to adjust the total amount by adding water, and then adjust the adjustment solution at low temperature for 1 to 12 days Jujube extract can be obtained by performing membrane filtration of the precipitates which were produced by standing to liver. In addition, the walnut extract is extracted by boiling water for about 30 minutes to 12 hours under a reflux apparatus after adding a certain amount of water to the walnut, filtered through a filter paper to take the filtrate and adjust the total amount by adding water, and then adjust the adjustment liquid at low temperature from 1 to The walnut extract can be obtained by performing membrane filtration of the precipitate produced by standing for 12 days. Then, the jujube extract and walnut extract obtained above may be mixed at a ratio of 1: 9 to 9: 1, respectively, to obtain a mixture of jujube and walnut extract of the present invention, that is, jujube extract and walnut extract.

Therefore, the present invention provides a cosmetic composition for moisturizing skin comprising the jujube extract and walnut extract obtained by the above method.

In addition, the cosmetic composition for moisturizing the skin according to the present invention, in addition to the above-mentioned extract, other moisturizing ingredients that can give a synergistic effect to the main effect within the range that does not impair the main effect of the present invention. It may also contain. Ceramide, glycerol, glycerin, etc. are mentioned as such a component.

Jujube extract and walnut extract according to the present invention is suitable for applying the dry powder of the eluate, the eluent, the eluate or the eluate as a skin moisturizing cosmetic formulation, but can be formulated in a conventional external preparation or cosmetic form. have.

The composition containing the jujube extract and the walnut extract of the present invention can be used in a variety of cosmetics, face wash and shampoo for skin moisturizing effect. Examples of products to which the composition can be added include cosmetics such as various creams, lotions and skins, and shampoos, rinses, cleansing agents, face washes, soaps, treatments, and cosmetics.

In addition, the skin moisturizing cosmetic composition containing the jujube extract and walnut extract of the present invention may take the form of a solution, an emulsion, a viscous mixture, and the like.

The cosmetic composition for moisturizing the skin according to the present invention is not particularly limited in the formulation, but for example, latex, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, Formulations such as patches, cosmetic liquids, cleansing foams, cleansing creams, cleansing water, soaps, sprays and the like.

In the cosmetic composition of each formulation, other components in addition to the above extract may be appropriately selected by those skilled in the art without difficulty depending on the formulation or purpose of use of other cosmetics.

Such formulations may contain skin absorption promoting substances to increase the moisturizing effect.                     

In addition, the cosmetic of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More preferably, 0.01 to 3 weight% is mix | blended.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the present invention is not limited only to these examples.

Example 1 Preparation of Jujube Extract

Take 10 g of jujube, add 100 ml of water each time, extract with boiling under reflux for 3 hours, filter with filter paper, filter the filtrate, adjust to 100 ml total with water, and leave the adjustment solution at 4 ° C for 6 days. The precipitates thus produced were subjected to 0.45 um membrane filtration to obtain jujube extracts.

Example 2. Preparation of Walnut Extract

Take 10 g of walnuts, add 100 ml of water each time, extract with boiling under reflux for 3 hours, filter with filter paper, filter the filtrate, adjust to 100 ml total with water, and leave the adjustment solution at 4 ° C for 6 days. The precipitates produced were subjected to 0.45 um membrane filtration to obtain walnut extracts.

Example 3 Preparation of Mixture of Jujube Extract and Walnut Extract

The jujube extract obtained in Example 1 and the walnut extract obtained in Example 2 were mixed in a 1: 2 ratio, that is, 100 g of jujube extract and 200 g of walnut extract, respectively (300 g) Was prepared.

Experimental Example 1. Induction effect of jujube extract and walnut extract on keratinocytes

In order to determine the effect of differentiation induction of keratinocytes of jujube extract and walnut extract were performed as follows.

First, put human keratinocytes to 5,000 cells / well in a 96-well microtiter plate containing KGM (Keratinocyte Growth Media) medium containing 1.0% fetal calf serum. After that, the cells were transferred to fetal calf serum-free KGM medium and incubated until 70-80% growth. Human cultured keratinocytes were placed in a culture flask and grown to about 80%. Thereafter, the jujube extract of Example 1, the walnut extract of Example 2, and the mixture of jujube extract and walnut extract of Example 3 in a ratio of 1: 2, respectively, were treated with 200 μl for 4 days, followed by urea or SDS And a reducing agent such as beta mercaptomethanol (β-mercaptoethanol) to remove the dissolved protein. Thereafter, the remaining stratum corneum measured peptide concentration at 310 nm and compared its absorbance values. At this time, the low calcium (0.05 mM) treated group without the extract was used as a negative control group, the high calcium (1.5 mM) treated group without the extract as a positive control, the test results are summarized in Table 1 below.

As a result, as shown in Table 1, the proliferative effect of keratinocytes was 31% for walnut extract, 57% for jujube extract, and 72% for the mixture of jujube extract and walnut extract.

As a result, keratinocyte differentiation inducing effect appears more in jujube extract than walnut extract, in particular, it can be seen that the effect is increased when using the jujube extract with walnut extract.

Material name Proliferative effect (5)  Jujube extract 57  Walnut extract 31  Mixture of jujube extract and walnut extract 72  Negative Control 0  Positive control group 100

Experimental Example 2. Skin barrier repair effect of jujube extract and walnut extract in animals

In order to determine the skin barrier recovery effect in the experimental animal of jujube extract and walnut extract was performed as follows. At this time, the skin barrier recovery effect was measured by the change of the epidermal moisture loss (TEWL: TransEpidermal Water Loss) with the damaged barrier state as 100. The epidermal moisture loss means that the water supplied from the dermis of the skin It is mainly diffused to the stratum corneum by passive diffusion and lost to the outside.

First, acetone is repeatedly applied to young female hairless mice 8-10 weeks after birth (Charles River) to impair the barrier function, and then the epidermal moisture loss (TEWL) is measured. (evaporimeter EP1, ServoMED) and reached to 4.0 g / m ² / h of each extract (jujube extract of Example 1, Walnut extract of Example 2 and jujube extract of Example 3 and Walnut extract of Example 3 Mixture) was applied to an area of 5 cm ² to measure epidermal moisture loss, and then the extent to which skin barrier function was restored by evaluating the degree of decrease was measured. The measurement was performed for 1 hour, 2 hours, 4 hours and It was performed after 8 hours. In addition, a group containing no extract was used as a negative control group, and a known lipid mixture (ceramide: cholesterol: fatty acid = 2: 1) was used as a positive control group. Skin barrier recovery effect was measured by the change of epidermal moisture loss (TEWL) with the damaged barrier state as 100 as described above, the results are shown in Table 2 below.

In Table 2, the mixture of jujube extract and walnut extract showed a nearly similar recovery effect when compared with the positive control. However, when treated only with jujube extract, it was relatively less skin barrier recovery effect than when treated only with walnut extract. Therefore, when the jujube extract and walnut extract are used together, it can be said that the skin barrier effect is increased.

Material name 1 hour later 2 hours later 4 hours later 8 hours later  Jujube extract 68.5 58.1 51.9 34.8  Walnut extract 65.8 54.0 49 39.2  Mixture of jujube extract and walnut extract 57.9 45.3 37.8 26.3  Negative Control 90.4 92.4 87.3 85.1  Positive control group 65.0 45.7 40.1 28.5

Experimental Example 3. Increased skin moisturizing effect of jujube extract and walnut extract through human experiment

In order to examine the skin moisturizing effect of jujube extract and walnut extract through the human experiment, the skin moisture increase effect experiment was conducted as follows, and a questionnaire about skin dryness improvement evaluation was conducted to the test subjects.

3-1. Skin moisture increase effect

The cosmetics prepared in Examples and Comparative Examples of the following formulation examples were selected for 140 women 20 to 40 years old with a bad skin, and given to Example 1 and Comparative Examples 1 to 4, respectively, and applied to the face and front half for 1 month twice a day It was made. At this time, the skin dryness is measured as the default value by using a moisture meter (corneometer, CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 ℃, 40% humidity) before starting the application. After 2 weeks, 5 weeks, the skin moisture was measured to evaluate the objective effect. At this time, the lipid mixture used in Experimental Example 2 was used as a positive control group, which did not contain an extract as a negative control group.

3-1-2. Formulation example (emulsifier type)

In the composition of Table 3, the oil and water phases were completely dissolved at 70 ° C., and emulsified at 7,000 rpm for 5 minutes to prepare a cream formulation.

Ingredients (content%) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Beads wax 2 2 2 2 2 Stearyl alcohol 5 5 5 5 5 Stearic acid 8 8 8 8 8 Squalene 10 10 10 10 10 Propylene Glycol Monostearate 3 3 3 3 3 Polyoxyethylene ether One One One One One Preservatives, Antioxidants dose dose dose dose dose glycerin 4 4 4 4 4 Propylene glycol 8 8 8 8 8 Triethylamine One One One One One Sodium Polyacrylate 0.2 0.2 0.2 0.2 0.2 Purified water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Jujube extract - - 2 - - Walnut extract - - - 2 - Mixture of jujube extract and walnut extract 2 - - - - Lipid mixture - - - - 2

3-1-3. result

As a result of experiments to increase the skin moisture as in 3-1, as shown in Table 4, it was confirmed that the skin moisture in all the mixture of jujube extract, walnut extract, jujube extract and walnut extract. However, in particular, it was confirmed that the skin moisture increase effect by the mixture of jujube extract and walnut extract is the most excellent.

Remarks Skin moisture growth rate (%) 1 week past 2 weeks past 3 weeks past Example 1 69 74 81 Comparative Example 1 11 14 17 Comparative Example 2 43 54 58 Comparative Example 3 39 50 54 Comparative Example 4 51 53 65

3-2. Skin Dryness Improvement Evaluation (Survey)

Subjective efficacy evaluation was conducted through a questionnaire survey for the test subjects of Experimental Example 3-1, and the results are shown in Table 5 below.

As a result, as can be seen in Table 5, the mixture of jujube extract and walnut extract in the subjective dryness improvement evaluation of the subjects obtained excellent results.

Therefore, it was confirmed that the skin moisturizing effect is increased by the mixture of the jujube extract as the keratin differentiation accelerator and the walnut extract as the water retaining substance.

Remarks % After use (%) Very good Good usually Inadequate Example 1 - 4 71 25 Comparative Example 1 44 34 20 2 Comparative Example 2 40 42 17 One Comparative Example 3 75 21 4 - Comparative Example 4 45 40 13 2

Hereinafter, a formulation example of the cosmetic composition will be described, but the present invention is only intended to be described in detail and not intended to limit it.

Soap manufacturers

Jujube extract and walnut extract of Example 3 ............ 1-20 (%)

Maintenance ...

Sodium hydroxide ...............

Sodium Chloride .........................................

Spices Quantity

The whole amount was 100 with purified water, and soap was prepared by the above blending ratio.

Lotion manufacturers

Jujube extract and walnut extract of Example 3 ............ 3.00 (%)

L-ascorbic acid-2-phosphate magnesium salt ......... 1.00

Water Soluble Collagen (1% Aqueous Solution) ... 1.00

Sodium Citrate ... 0.10

Citric Acid ........................................... 0.05

Licorice Extract ......... 0.20

1,3-butylene glycol ... 3.00                     

The whole amount was 100 with purified water, and a lotion was prepared by the above blending ratio (%).

Cream manufacturers

Jujube extract and walnut extract of Example 3 .................. 1.00 (%)

Polyethylene Glycol Monostearate ............... 2.00

Self-emulsifying glycerin monostearate ......................... 5.00

Cetyl Alcohol ... 4.00

Squalene ......................................... 6.00

Glyceryl tri2-ethylhexanoate ......... 6.00

Sphingose Sugar Lipids ......................................... 1.00

1.3-Butylene Glycol ... 7.00

The total amount was 100 with purified water, and a cream was prepared at the blending ratio (%).

Pack manufacturer

Jujube extract and walnut extract of Example 3 ...... 5.00 (%)

Polyvinyl Alcohol ... 13.00

L-ascorbic acid-2-phosphate magnesium salt ......... 1.00

Lauroylhydroxyproline ............... 1.00

Water Soluble Collagen (1% Aqueous Solution) ... 2.00

1,3-butylene glycol ... 3.00                     

Ethanol ......................................... 5.00

The total amount was 100 with purified water, and a pack was prepared at the blending ratio (%).

Essence

Jujube extract and walnut extract of Example 3 ....... 2.00 (%)

Hydroxyethylene Cellulose (2% Aqueous Solution) ... 12.00

Xanthan gum (2% aqueous solution) ....................... 2.00

1,3-butylene glycol ......... 6.00

Dark glycerin ... 4.00

Sodium hyaluronate (1% aqueous solution) ....................... 5.00

The whole amount was 100 with purified water, and a cosmetic liquid was prepared at the blending ratio (%).

As can be seen from the above, the cosmetic composition containing the jujube extract and walnut extract according to the present invention has an excellent effect on skin moisturizing. In particular, the cosmetic composition uses herbal herbal extracts, promotes the differentiation of the stratum corneum of the skin cells, restores the barrier function of damaged skin, and also has the effect of increasing and maintaining the skin's moisturizing effect in human experiments, containing jujube extract and walnut extract It can be usefully used as a cosmetic composition for skin moisturizing.

Claims (6)

A cosmetic composition for moisturizing skin containing a mixture of jujube extract for promoting keratin differentiation and walnut extract for promoting keratin moisture retention. delete The cosmetic composition for skin moisturizing according to claim 1, wherein the cosmetic composition contains jujube extract at a concentration of 0.001-20% by weight in the total composition. The cosmetic composition for moisturizing skin according to claim 1, wherein the cosmetic composition contains walnut extract at a concentration of 0.001-20% by weight of the total composition. The cosmetic composition for moisturizing skin according to claim 1, wherein the composition ratio of the jujube extract and the walnut extract in the cosmetic composition is contained in a weight ratio of 1: 9 to 9: 1. According to claim 1, wherein the cosmetic composition is an emulsion, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence, cleansing foam, cleansing cream, cleansing water Cosmetic composition for moisturizing the skin, characterized in that the formulation of soap, spray.