KR100997442B1 - Cosmetic composition containing the extract of phragmites japonica steud - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Phragmites japonica steud extract is provided to ensure antioxidation effect, whitening effect, moisturizing effect, and anti-inflammation. CONSTITUTION: A cosmetic composition contains Phragmites japonica steud extract as an active ingredient. The solvent for extracting Phragmites japonica steud is purified water, methanol, ethanol, glycerol, ethylacetate, butylenes glycol, propylene glycol, dichloromethane, hexane or mixture thereof. The cosmetic composition is used for antioxidation, skin whitening or moisturizing. The cosmetic composition is used in the form of toner, gel, aqueous liquid, cream, essence, emulsion oil in water(O/W) or water in oil(W/O) or ointment.

Description

Cosmetic composition containing the extract of Root Root {Cosmetic composition containing the extract of Phragmites japonica steud}

The present invention is Phragmites japonica Steud ) relates to a cosmetic composition containing the extract as an active ingredient, more specifically, an antioxidant effect, skin whitening effect, skin moisturizing effect, cytotoxic effect and The present invention relates to a cosmetic composition having an excellent anti-inflammatory effect.

Human skin has various physical and chemical changes during the aging process, and the causes are largely divided into internal aging and photo-aging, and research on this has been actively conducted. In other words, the aging of the skin may be caused by ultraviolet rays, stress, disease state, environmental factors, wounds, activation of free radicals with age, and when this condition is intensified, it destroys the antioxidant defense network existing in the living body, Damage to cells and tissues promotes adult disease and aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of protein is caused by the breakdown of collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are the skin's connective tissues, resulting in severe excessive inflammatory reactions and disruption of skin elasticity. This leads to a situation of reduced immune function.

Therefore, it protects the cell membrane by eliminating the free radicals generated by metabolic process of the body, irradiation by UV irradiation, or inflammatory reaction.Also, damaged cells should be regenerated by active metabolism to proliferate the cells. You can recover quickly and maintain healthy skin.

Next, skin aging due to pigmentation may be mentioned, and pigments affecting skin color include melanin, melazoid, carotene, hemoglobin, and the like. The most important thing is melanin, the biggest factor affecting biosynthesis is ultraviolet radiation and hormone secretion in the body. Melanin plays an important role in absorbing or scattering ultraviolet rays to prevent damage to the skin from ultraviolet rays. There is no special maximum absorption wavelength and it absorbs light in all areas. In addition, it is excellent in removing active oxygen species, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in melanin structure, and melanin itself shows the properties of free radicals. Pray.

The biosynthesis of melanin is brown, eumelanin through a series of oxidation processes starting with the conversion of tyrosine, an amino acid, from the melanocytes of melanocytes to tyrosinase and conversion to dihydroxy phenylalanine. It is formed of a polymer of). This biosynthesis process is carried out in a special type of brown intracellular organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the dendritic end, and then into the cytoplasm by the phagocytosis of keratinocytes. Accumulate around the nucleus of the site.

Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A 6-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150), and some extracts are already tyrosinase. Although it is used as a whitening cosmetic because it has an inhibitory activity, its use is limited due to poor stability in cosmetic formulations, discoloration due to degradation, odor occurrence, efficacy at the biological level, unclear effect and safety issues. .

On the other hand, in the human body, the skin is an organ that functions as a protective film to protect the living body from the external environment, and prevents the loss of biological components, that is, water or electrolyte, and prevents harmful substances from entering the human body from the outside. Do it. Such skin is largely divided into epidermal layer, dermal layer and subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. Since the outermost keratinocyte layers of skin are in direct contact with the external environment, they require strong resistance to physical, chemical, or permeation of substances, and prevent the loss of moisture from the body to the outside. At the same time, it should maintain flexibility by retaining adequate moisture on its own.

In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, which is about 10 to 30% in the keratinocyte layer. The water supplied from the dermal layer is mainly moved up the stratum corneum by passive diffusion and finally discharged to the outside. This is called Trans Epidermal Water Loss (TEWL), and it is known that it is the sebaceous component and the epidermal lipid which are the lipid membrane components of the keratinocyte layer to maintain the appropriate level of transdermal moisture loss. In the keratinocyte layer, a substance having a hydrophilic water retention ability called natural moisturizing factor (NMF) is known to play an important role in moisturizing the skin. When the moisture of the normal keratinocyte layer is maintained about 10 to 30%, the skin is smooth and soft, and the body protects normally.

However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the body loses its protective function, causing aging. For example, in the case of dry skin, the aggregation force of keratinocytes is weakened, and scaling phenomenon in which keratinocytes are peeled off like a piece of scale from the skin surface occurs. Thus, the dryness of the skin is caused by the moisture content of the keratinocyte layer. This is because it is less than normal skin. In addition, even healthy skin may suffer from a lack of moisture due to harsh external environment, ie, wind, cold weather, sunlight, face wash, shaving, and the like, and thus worsens the skin condition.

Therefore, it is very important to maintain the moisture content of the keratinocyte layer of the skin properly. To this end, cosmetics such as sebum-like components, NMF components, or moisturizers such as polyols were added and used. For example, glycerin, solitol, and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisturizing properties, but are highly sticky, making them unpleasant when used, and having two hydroxyl groups (OH groups), 1, 3-butylene glycol may cause side effects on the skin. In addition, other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic and thus impair the emulsion stability of cosmetics, and also moisturize amino acids, collagen and elastin. There is a capacity, but the moisturizing ability was limited. The ability to retain moisture in the stratum corneum is controlled by a natural moisturizing factor (NMF) consisting of sebum, amino acids, lactic acid, urea and inorganic salts. The development of high-efficiency substances is one of the major research challenges in cosmetics.

In recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemicals. Natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural ingredients has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing. Thus, the present inventors have studied the possibility of applying to cosmetics in a variety of natural products, and as a result, moon root grass extract exhibits an excellent antioxidant effect, and also has excellent skin whitening effect, skin moisturizing effect, cytotoxic effect and anti-inflammatory effect. It has been found that the efficacy as a cosmetic can be expected to complete the present invention.

It is an object of the present invention to provide a natural extract that is applicable to skin cosmetics and is harmless to the human body, and has excellent safety as well as an excellent antioxidant effect and a cosmetic composition containing the same as an active ingredient.

In addition, an object of the present invention is to provide a natural extract and a cosmetic composition containing the same as an active ingredient, which is very excellent in antioxidant effect and also very excellent in skin whitening and skin moisturizing effect.

In addition, an object of the present invention is to provide a natural extract and a cosmetic composition, which is also excellent in cytotoxicity and anti-inflammatory effects.

In order to achieve the above object, the present invention is Phragmites japonica steud ) characterized in that the cosmetic composition containing the extract as an active ingredient.

In addition, in the present invention, it is characterized in that the content of 0.001 to 30.0 wt.

In addition, in the present invention, the extracting solvent of the Moon root grass extract is characterized in that it is selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane and mixtures thereof. .

In addition, in the present invention, the cosmetic composition is characterized in that for antioxidant.

In addition, in the present invention, the cosmetic composition is characterized in that for skin whitening.

In addition, in the present invention, the cosmetic composition is characterized in that for moisturizing the skin.

In addition, in the present invention, the cosmetic composition is a formulation selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) or water-in-oil (W / O) type emulsion and ointment. It features.

Hereinafter, the means for solving the problems of the present invention will be described in detail.

Dalppuri Pool (Phragmites japonica steud ) is a perennial herb that is a monocotyledonous plant belonging to a family of rice plants, and is called perennial herb that grows on the sandy grounds of streams. Roots grow to the ground and take root from nodes. The leaves are revived, the tip is long, and the purple color is on the upper part of the leaf, and the lobes are hairy. Its roots are exposed to the ground, they grow and grow, and the leaves tend to run in one direction.

Root roots have long been used as a medicine in Chinese medicine and in private. Diuretic, detoxification, food poisoning, and plants are said to be effective. Contains sugar, gum, protein, inorganic salts, etc. Diuretic, hemostatic, sweating, anti-inflammatory, Jigal, detoxification, jinto (鎭 吐) has a variety of pharmacological effects. In addition, the roots of the roots are sweet in taste, cool in nature and act on menopause and stomach. Lowers the fever, increases the fluid, urinates well, eliminates hangovers and protects the liver. In addition, the roots of the roots are excellent when you eat or pretend to eat meat, such as pork or chicken. In addition, the roots of the roots are known to have a strong detoxifying effect and treatment of radiation poisoning and thereby leukopenia. In addition, moon root grass is also commonly used in the treatment of diabetes, jaundice, various cancers, vomiting, chronic peritonitis, pulmonary fever relief, edema, arthritis, cystitis, urinary pain.

In addition to these medicinal plants, moonroot grass is used for ornamental, medicinal and crafts for water purification and landscape composition of rivers. As the reeds are known to have a great effect on the water quality of rivers like reeds, they have recently been used for water purification and landscape composition of urban rivers.

Ingredients include coixol and protein, fat, carbohydrates and asparagin in the roots, as well as cellulose, lignin, xylan and ash. It is known that cellulose, pentosan, lignin, ash, moisture, and vitamin C are contained in parts such as leaves, flowers, and shells.

Moon root grass extract used in the present invention can be obtained through a variety of methods known in the art, preferably a leaching solution obtained by leaching, leaching from the moon root grass starch, or a concentrate obtained by concentrating the leaching solution again or partially, Or the extract extracted by drying the concentrate again and the chemical itself having the main effect contained in the extract. For example, the extract of the myrtle root extract according to the present invention is extracted with 10-95% (V / V) ethanol aqueous solution at room temperature, cold-dried and filtered the resulting suspension at 50 ℃ or less Concentrated under reduced pressure and may be prepared by lyophilization of the reduced pressure concentrate. In addition, as the extraction solvent of the present invention, those selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane and mixtures thereof can be preferably used.

In addition, the extract of the root of the present invention includes not only the above-mentioned extraction method, but also extracts extracted by other methods. Separation using, for example, an ultrafiltration membrane having a constant molecular weight cut-off value; Fractions obtained by various purification methods additionally performed, such as separation by chromatography prepared for separation according to various chromatography, for example, size, charge, hydrophobicity or affinity, are included in the extract of the root of the present invention.

In addition, in the present invention, the moon root grass which is an extraction material can be used by drying it or used as it is, and preferably it is used by drying. As a drying method, natural drying, shade drying, or a hot air drying method can be used. In addition, in order to facilitate the extraction may be pulverized.

In addition, although the extract of the root of the present invention may be used in a liquid state, it may be prepared and used in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

On the other hand, the content of the extract of the roots of the present invention is preferably 0.001 to 30.0% by weight based on the freeze-drying weight of the total cosmetic composition. When the content of the extract is less than 0.001% by weight, there is almost no skin improvement effect, and when the content of the extract is more than 30.0% by weight, it is not economical because the degree of increase of the effect on the content is insignificant.

In addition, the cosmetic composition according to the present invention may contain, in addition to the above-mentioned extract, other components that can give a synergistic effect to the main effect, preferably within the range not impairing the main effect of the present invention. Even in this case, the extract may be present in the composition in an amount of 0.001 to 30.0 wt% based on the lyophilized weight of the composition. If the myrtle extract is present in the form of a solution containing the extract, those skilled in the art will be able to adjust the amount of this solution in the composition according to the invention within the concentration range.

On the other hand, the cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art. In one embodiment, the compositions of the present invention are oil dispersions in an aqueous phase using an aqueous, aqueous-alcoholic or oily solution, oil-in-water or water-in-oil or multiple emulsions, aqueous or oily gels, liquids, pasty or solid anhydrous products, globules. It may be in the form of water, more preferably in the form of lipid vesicles in ionic and / or nonionic form.

The composition may also be somewhat fluid and may have the appearance of a white or colored cream, ointment, milk lotion, serum, essence, paste or mousse. It may optionally be applied to the skin in the form of an aerosol or may be in the form of a solid, such as a stick. It may be used as a skin care product and / or makeup product.

In addition, the cosmetic compositions of the present invention can be used in the field of cosmetics such as conventional auxiliaries such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants and dyes. It may contain by a method known in the art. The amounts of these various adjuvants are amounts conventionally used in the art to which this invention pertains, for example from 0.01% to 20% by weight relative to the total weight of the composition. Depending on its nature, such adjuvants may be incorporated into the fatty phase, aqueous phase, lipid vesicles and / or nanoparticles. In any case, the adjuvant and its proportions will be chosen so as not to adversely affect the desirable properties of the cosmetic composition according to the invention.

The present invention relates to a cosmetic composition containing the extract of the myrtle root as an active ingredient, the composition of the present invention can be used as an antioxidant, skin lightening agent, skin moisturizing agent, and also has excellent cytotoxicity and anti-inflammatory effect.

The present invention can be applied to skin cosmetics and harmless to the human body can provide a natural extract and a cosmetic composition containing the same as an active ingredient with excellent safety as well as excellent antioxidant effect.

In addition, the present invention can provide a natural extract and a cosmetic composition containing the same as an active ingredient, which is very excellent in antioxidant effect and also very excellent in skin whitening and skin moisturizing effect.

In addition, the cosmetic composition of the present invention is very excellent in cytotoxicity and anti-inflammatory effect.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes all modifications of equivalent technical spirit.

Production Example  1: Preparation of Moon Root Extract

The dried and dried Sakura root outpost was refluxed three times for 5 hours with an aqueous 95% (V / V) ethanol solution, cooled and then filtered with Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 ° C or lower. The concentrated root extract was prepared by dispersing and dissolving at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol so as to contain 0.001 to 30.0% by weight of the reduced pressure concentrate and lyophilized product.

Test Example  One: NBT Determination of Antioxidant Effects of Moon Root Extracts Using

In this test example, the antioxidant activity of retinol and BHT was compared with a sample in laboratory conditions to determine the antioxidant effect of the extract obtained from Preparation Example 1 of the present invention, the antioxidant activity was measured using the NBT method.

In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase was measured by NBT method, and the effect of removing the active oxygen by the test substance, that is, the effect of scavenging the active oxygen, was evaluated. Free radicals were generated by xanthine and xanthine oxidase, and the reactive oxygen scavenging rate was measured by measuring the blue color produced by reaction of the reactive oxygen with Nitro Blue Tetrazolium (NBT) at a wavelength of 560 nm. . Specific measurement method of this test example is as follows.

0.05M Na2CO3 ------------------------------------ 2.4ml

2.3mM xanthine solution ---------------------------------- 0.1ml

3.3mM EDTA Solution ------------------------------------ 0.1ml

4.BSA solution ---------------------------------------- 0.1ml

5.0.72 mM NBT solution ---------------------------------- 0.1ml

6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl2 Solution ----------------------------------- 0.1ml

① 1, 2, 3, 4, and 5 were added to the Bayer bottle, and 0.1 ml of the sample solution was added thereto and left at 25 ° C. for 10 minutes.

② After the addition of the above 6, the mixture was stirred quickly and incubated for 20 minutes at 25 ° C.

(3) Then, 7 was added to stop the reaction, and the absorbance St at 560 nm was measured.

④ In the blank test, absorbance Bt was measured in the same manner as described above using distilled water instead of the sample solution.

⑤ Also, the blank of the sample solution was operated in the same manner using distilled water instead of the above 6 to measure the absorbance Bo.

The result of the antioxidant effect was calculated by the following Equation 1, the results are shown in Table 1.

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme in sample solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

As shown in Table 1, it was confirmed that the excellent antioxidant effect in all the samples tested at 0.1% treatment concentration. In addition, the antioxidant effect of the extract of the root of the root was 90%, it was confirmed that the antioxidant effect is similar or superior to the retinol and BHT.

Sample Name Treatment concentration (%) Antioxidative effect(%) Moon root extract 0.1 90 Retinol 0.1 89 BHT 0.1 86

Test Example  2: DPPH Determination of Antioxidant Effects of Moon Root Extracts Using

In this test example, antioxidant activity of green tea extract and antioxidants such as vitamin E were measured by DPPH method in order to measure the antioxidant effect of the extract of Moon root grass obtained in Preparation Example 1 of the present invention. .

In the DPPH method, antioxidant activity was measured by reducing power using a free group called 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (DPPH). The free radical scavenging rate was measured at a wavelength of 560 nm by comparing the degree to which the DPPH was reduced by the test substance to reduce the absorbance.

As the reagent used, a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

The specific measuring method is as follows.

① 0.15 ml of 0.1 mM DPPH solution was added to a 96-well plate, and 0.15 ml of the sample solution was quickly stirred and incubated at 25 ° C. for 10 minutes.

② The absorbance St at 560 nm was then measured.

③ In the blank test, the absorbance Bt was measured in the same manner as described above using distilled water instead of the sample solution.

④ Again, the blank of the sample solution was operated in the same manner using methanol instead of the 0.1 mM DPPH solution to measure the absorbance Bo.

The result of the antioxidant effect was calculated by the following Equation 2, the results are shown in Table 2.

St: absorbance at 560 nm after free radical scavenging of sample solution

Bt: absorbance at 560 nm after free radical scavenging of blank test solution

So: Absorbance at 560 nm before reaction without free radicals in sample solution

Bo: absorbance at 560 nm before reaction without free radicals in blank test solution

As shown in Table 2, it was confirmed that the Moon root grass extract of the present invention has a superior antioxidant effect than the green tea extract and vitamin E at 0.01% concentration.

Sample Name Treatment concentration (%) Antioxidative effect (%) Moon root extract 0.01 65 Green Tea Extract 0.01 39 Vitamin E 0.01 35

Test Example  3: Determination of Cytotoxicity Relief by Ultraviolet Irradiation of Moon-Root Extract

This test example was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of the extract of Moonroot herb obtained in Preparation Example 1 of the present invention.

Fibroblasts (fibroblasts) were placed in a 24-well test plate 1 x 10 ⁵ and attached for 24 hours. Each well was washed once with PBS and 500 ul of PBS was added to each well. The fibroblasts were irradiated with UV 10mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 ml) was added. After treatment with the myrtle extract to be evaluated it was incubated for 24 hours. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg / ml) were added to each well, followed by incubation in a CO ₂ incubator at 37 ° C. for 2 hours. The medium was removed and 500 μl of isopropanol-HCl (0.04 N) was added. After shaking for 5 minutes, the cells were lysed and 100 μl of the supernatant was transferred to a 96-well test plate, followed by measurement of 565 nm absorbance in a microplate reader. Cell survival rate (%) was measured by Equation 3 below, and cytotoxicity relaxation rate by ultraviolet rays was calculated by Equation 4 below.

Bo: 565 nm absorbance of wells that only reacted with cell culture media

Bt: 565 nm absorbance of the wells that developed the colorless reaction wells

St: 565 nm absorbance of the wells that reacted the sample treated wells

Bo: cell viability of wells without UV irradiation and no sample treatment

Bt: cell viability of wells that were not irradiated with UV light

St: Cell survival rate of wells treated with UV

As a result of the experiment, as shown in Table 6, it was found that the myrtle root extract of the present invention effectively reduced cytotoxicity caused by ultraviolet rays by 66% at 0.02% treatment concentration, thereby effectively protecting cytotoxicity caused by ultraviolet rays even at low concentrations.

Sample Name Treatment concentration (%) Cytotoxic alleviation rate (%) Moon root extract 0.01 42 Moon root extract 0.02 66

Test Example  4: Determination of Inflammatory Cytokine Expression Inhibition by Ultraviolet Irradiation of Moon-Root Extract

This test example is to evaluate the degree of inhibition of the expression of inflammatory cytokines expressed by ultraviolet irradiation in order to confirm the anti-inflammatory effect of the extract of the Root Root obtained in Preparation Example 1 of the present invention.

Fibroblasts isolated from human epidermal tissue (Fibroblast) were placed in a 24-well test plate ⁵ x 10 ⁴ and attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with 10 mJ / cm 2 of ultraviolet rays using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), after which PBS was removed and cell culture medium (FMEM was not added to DMEM). Medium) 350 μl was added. After treatment with the Root Root Extract to be evaluated therein was incubated for 5 hours. 150 µl of the culture supernatant was taken to quantify IL-1α to determine the inhibitory effect of inflammatory cytokine expression of the extract of Moonflower. The amount of IL-1α was quantified using Enzyme-linked Immunosorbent Assay (ELISA), and the production rate of IL-1α was calculated by the following Equation 5.

Bo: amount of IL-1α produced in wells not irradiated with UV

Bt: amount of IL-1α produced in wells that were not irradiated with UV light

St: Production of IL-1α in Wells Treated by Ultraviolet Irradiation

As a result of the experiment, as shown in Table 7, the extract of the present invention is effective to prevent the inflammation caused by ultraviolet rays at low concentrations by inhibiting 72% at 0.02% concentration of IL-1α, which is an inflammatory cytokine caused by ultraviolet rays. I could see.

Sample Name Treatment concentration (%) Inhibitory rate of inflammatory cytokine expression (%) Moon root extract 0.01 38 Moon root extract 0.02 72

Test Example  5: B16F1 Melanosite  Determination of Melanin Inhibitory Effect of the Extracts from the Extracts

This test example is to determine the whitening effect by looking at the degree of inhibition of melanin production on B16F1 melanocytes in order to confirm the whitening effect of the extract of the Roots of the root obtained in Preparation Example 1 of the present invention.

The B16F1 melanocytes used in this test example are cell strains derived from mice, and cells that secrete a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. The B16F1 melanosite used in this test example was used after being sold from ATCC (American Type Culture Collection, Accession No .: 6323).

The melanin biosynthesis inhibitory effect of B16F1 melanocytes was measured as follows.

B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 x 10 ⁶ per well, and cells were attached and then incubated for 72 hours by treating the samples at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan: Cancer Res. , 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melt the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), measure the absorbance of melanin at 405 nm with a microplate reader, and then quantify the melanin in the sample. Percent inhibition of production was measured. Melanin inhibition rate (%) of B16F1 melanocytes was calculated by the following equation (6), IC ₅₀ value is the concentration of the substance inhibiting 50% melanogenesis.

A: Melanin amount in wells without sample

B: Melanin amount in the wells to which the sample was added

As a result of testing the melanin production inhibitory effect of B16F1 melanocytes, as shown in Table 5, the IC ₅₀ value of the extract of the moon root grass of the present invention is 0.08%, the existing whitening agents hydroquinone, arbutin, oil-soluble licorice extract, lettuce skin It can be seen that the similar or superior effect compared to the extract.

Sample Name Melanin production inhibitory effect (IC ₅₀ ) Moon root extract 0.08% Hydroquinone 0.05% Arbutin 0.30% Lettuce extract 0.10% Soluble Licorice Extract 0.03%

Test Example  6: Determination of Skin Whitening Effect of Moonroot Herb Extract

In this test example, the cosmetics containing the extract of the Root Root obtained in Preparation Example 1 of the present invention was prepared, and the skin whitening effect of humans was evaluated by performing a comparative experiment with Comparative Example 1.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 6 below. First, the phase b) recorded in Table 6 was heated and stored at 70 ° C. A) phase was added thereto, followed by preliminary emulsification to uniformly emulsify with a homomixer, and then gradually cooled to prepare a cream (Example 1, Comparative Example 1).

Ten experimenters (women aged 20-35 years) were applied to the right side of the face with the cream prepared in Example 1 and the left side of the face with the cream prepared in Comparative Example 1 twice a day for 2 consecutive months. . After completion of the test, the change of the brightness of the color (ΔL) was measured by using an image analyzer and the color change of the skin on both left and right sides of the face using a chromameter (Minolta CR300). Subjective visual observation was performed and the effect was measured according to the following classification. The results are shown in Table 7 below. At this time, the degree of whitening efficacy was classified into the following seven ratings.

<Whitening efficacy evaluation criteria>

-3: very worse

-2: worse

-1: slightly worse

0: no change

1: slightly improved

2: improve

3: highly improved

As shown in Table 7, the facial skin of the experimenter coated with the cream containing the extract of the root of the present invention was found to have an excellent whitening effect.

Raw material Example 1 Comparative Example 1 end






Stearyl alcohol 8 8 Stearic acid 2 2 Stearic cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 Glyceryl Monostearic Acid Ester 2 2 Liquid paraffin 5 5 I


Moon root extract 5 - Propylene glycol 5 5 glycerin 4 4 Purified water Until 100 Until 100

Note) Unit: Weight%

Skin-colored
Change in brightness (ΔL)
Skilled
Objective evaluation Subject's
Subjective evaluation Example 1 Comparative Example 1 Example 1 Comparative Example 1 medium 3.4 2.7 1.8 2.9 1.7

ΔL = skin brightness after test-skin brightness before test

Test Example  7: Determination of Moisturizing Effect of Moon-root Extract

In this test example, a cosmetic composition containing the extract of the myrtle root obtained in Preparation Example 1 of the present invention was prepared and the skin moisturizing effect of the human subject was evaluated by comparative experiments with Comparative Examples 2 to 5.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 8. First, the phase b) recorded in Table 8 was heated and stored at 70 ° C. A) phase was added thereto, followed by preliminary emulsification to uniformly emulsify with a homomixer, and then gradually cooled to prepare a cream (Example 2, Comparative Examples 2 to 5). Example 20 and Comparative Examples 2 to 5 were applied to the lower part of the lower arm (part of the lower arm), and after about 90 minutes, ΔHydration was evaluated by the Corneometer (CM 820) for the moisturizing ability before and after application. .

Raw material Example 2 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5

end



Stearyl alcohol 8 8 8 8 8 Stearic acid 2 2 2 2 2 Stearic cholesterol 2 2 2 2 2 Squalane 4 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 3 Glyceryl Monostearic Acid Ester 2 2 2 2 2

I

Moon root extract 5 - - - - glycerin - - 5 - - Na-hyaluronic acid salt - - - 5 - 1,3-butylene glycol - - - - 5 Purified water Until 100 Until 100 Until 100 Until 100 Until 100

Note) Unit: Weight%

division Example 2 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Moisturizer Types Moon Root Extract - glycerin Na-hyaluronic acid salt 1,3-butylene glycol Corneometer
(Hydration) 16 5 11 13 3

As shown in Table 9, it was found that the moisturizing effect is very excellent in the facial skin of the experimenter applying the cream containing the extract of the root of the present invention.

Claims (7)

Dalppuri Pool (Phragmites japonica steud ) Cosmetic composition comprising the extract as an active ingredient. The method of claim 1,
Cosmetic composition, characterized in that containing the Moon root grass extract 0.001 to 30.0% by weight based on the total lyophilized weight. The method of claim 1,
The extracting solvent of the extract of the moon root grass is a cosmetic composition, characterized in that selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane and mixtures thereof. 4. The method according to any one of claims 1 to 3,
Cosmetic composition, characterized in that for antioxidant. 4. The method according to any one of claims 1 to 3,
Cosmetic composition, characterized in that for skin whitening. 4. The method according to any one of claims 1 to 3,
Cosmetic composition for moisturizing the skin. 4. The method according to any one of claims 1 to 3,
Cosmetic composition, characterized in that the formulation is selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) or water-in-oil (W / O) type of emulsion and ointment.