KR100371504B1 - Cosmetics composition comprising extract powder with vitis vinifera l.fermented solution and having whitening and anti-wrinkle effect - Google Patents

Abstract

The present invention relates to a cosmetic composition for whitening and anti-wrinkle, characterized in that the powdered grape fermentation extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition, according to the previously developed arbutin, ascorbic acid, etc. A grape fermentation broth extract having a much superior tyrosinase inhibitory effect, that is, a whitening effect, can be obtained, and a cosmetic composition containing grape fermentation extract having a very excellent effect in terms of whitening effect, anti-wrinkle effect and stability can be obtained.

Description

COSMETICS COMPOSITION COMPRISING COMPRISING EXTRACT POWDER WITH VITIS VINIFERA L.FERMENTED SOLUTION AND HAVING WHITENING AND ANTI-WRINKLE EFFECT}

The present invention relates to a cosmetic composition having a whitening and anti-wrinkle effect, and more particularly, to a cosmetic composition having a whitening and anti-wrinkle effect by containing a grape fermentation extract.

The skin is part of the body and, as it ages, it ages like any other living thing. The theory that is currently drawing attention to the theories based on biological research on aging is the free radical theory, ie cell disorders caused by lipid peroxide. The theory that free radicals are involved in aging phenomena was first claimed by Harroad in 1956 and has since been supported by many comments on lipid peroxide. However, the aging mechanism of the skin is not explained by any one theory. Previous studies and ongoing research have shown that it is almost impossible to regenerate the skin at the end of its growth phase by rebuilding its active skin structure, but it is possible to make more active improvements to the wrinkles that have already been produced, resulting in more elasticity. It turns out to be able to make skin smooth and smooth.

In Korea, the aging society is rapidly advancing as the medical and public health benefits are improved. At the beginning of the 21st century, one out of four people is expected to have an aging society that is over 65 years old. As a result, the quality of life is being improved, and the desire for the maintenance of youth is increasing, and the demand for cosmetics, especially anti-wrinkle cosmetics, has increased.

In this regard, anti-wrinkle agents known so far, such as moisturizer form to improve the wrinkles by improving the wrinkles by moisturizing dry skin to restore the abnormality of keratin accompanying the dry skin to normal to improve skin elasticity The substance which improves wrinkles, the substance which has an antioxidant effect, and the substance which suppresses transdermal moisture evaporation.

Among these, as a moisturizer which has an anti-wrinkle effect, hyaluronic acid, sodium chondroitin sulfate, and lactic acid are mentioned, Retinoic acid widely used for medicine is known to be excellent in anti-wrinkle effect.

However, retinoic acid has been reported as a side effect of reddening the skin and sensitive reaction by ultraviolet light, there is a problem that the effect appears only after 6 months of continuous use.

In addition, alpha hydroxy acid is also known as an anti-wrinkle agent (Korean Patent Publication No. 96-191), but this also accompanied the side effects of skin irritation such as itching, erythema, and sensitive reaction by ultraviolet rays, satisfying the safety aspects of the product. Since it cannot be made, there is a problem that the value as a cosmetic is low.

Moreover, the whitening effect in cosmetics aims at preventing melanin pigment from depositing on skin and suppressing the formation of spots and freckles. This melanin pigment is converted into melanin polymer from tyrosine or dopa by tyrosinase and tyrosinase, and is subjected to an automatic oxidation reaction to a melanin polymer. Therefore, research and development on the substance blocking the melanin pigment production pathway and cosmetics containing the same are also being actively conducted, and materials used for this purpose include ascorbic acid, kojic acid, arbutin, hydroquinone, etc. The Journal of the Society of Cosmetic Scientists, 1997.Vol. 23, No. 1), use of these substances is limited due to factors such as insufficient skin whitening effect in terms of safety for skin and stability in cosmetic formulation.

On the other hand, Vitis vinifera is a vine belonging to the genus Vitiaceae, which has been known to have urinary action, diarrhea, tonic action, metabolism promotion and lowering acidity of gastric juice. (Wonwon, 1984), wine has also been used to enhance appetite, tannin-rich red wine for diarrhea, and tonic (7 Korean Revisions, Moon Sungsa, 1998). It has been reported to increase antioxidant activity with respect to antioxidant activity in human blood.

Grapes have also recently been identified with grape seed extract (Korean Patent Publication No. 2000-0034037) as a tyrosinase inhibitor and whitening cosmetic composition (Korean Patent No. 1999-0085941) as a tyrosinase inhibitor in relation to the whitening effect. A method of preparing grape seed extract (Korean Patent Publication No. 1999-030352) and the like are known. However, the above methods extract the dried grapes (seeds, flesh, skins) at high temperature using water or various organic solvents by applying a temperature even before eluting the active ingredients. It can be destroyed and not show its effects, as well as skin irritation due to residual alcohol caused by organic solvent extraction.In case of using extract by water extraction for cosmetics, There is a disadvantage that the preservative treatment must be performed separately.

On the other hand, the grape fermentation extract extracted by fermenting the natural product grape itself is powdered after the active ingredient elution is completed, which is strong in heat resistance and photooxidation, and when the extract is used in cosmetics, the effective ingredient is extracted as it is, which maximizes the effect to be obtained. have.

In this regard, the present applicants are safe for the skin and have excellent anti-wrinkle effect due to tyrosinase inhibitory effect, in view of the problems described above in using the conventional whitening or anti-wrinkle effect in cosmetics and medicines. As a result of constant research to obtain cosmetics having excellent stability as a product, when the grape fermented extract extract is used in cosmetics, it was found that not only very good whitening and anti-wrinkle effects were obtained but also almost no skin irritation, thus completing the present invention. Was done.

Accordingly, it is an object of the present invention to provide a cosmetic composition excellent in whitening and anti-wrinkle efficacy without skin irritation.

1 is a graph showing the results of the whitening effect test for Preparation Example 3 containing the grape fermentation broth extract of Example 1 and Comparative Preparation Example 3 containing the grape extract of Preparation Example 1, A is an Example Treatment site of Preparation Example 3 containing the grape fermentation broth extract of 1, B is the treatment site of Preparation Example 5 containing the grape fermentation broth extract of Example 1, except that C does not contain the grape fermentation broth extract of Example 1 Is the treatment site of Preparation Example 3 of the same composition, D is the experimental result of the treatment site of Comparative Preparation Example 3 containing the grape extract of Comparative Example 2,

Figure 2 is a photograph of the results of testing the degree of inhibition of melanin biosynthesis of the grape fermentation broth extract using the Streptomyces bikiniensis strain.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition for whitening and anti-wrinkle, characterized in that the powdered grape fermentation extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition.

Hereinafter, the content of the present invention in more detail.

When the grapes are fermented and matured, the glucose and fructose components in the grapes are changed into alcohols, which can extract a significant amount of active ingredients such as flavonoids, vitamin C, vitamin P, amino acids, and catechins. It is possible to obtain an excellent whitening and anti-wrinkle effect even if not, the present invention uses this effect.

Grape fermentation broth extract used in the present invention, not only grapes (vitis vinifera Linne, grape vitaceae) but also other varieties (grape, Vitaceae, Gibong, Pione, Olympia, Delaware, etc.), without using a solvent It is extracted by fermenting and ripening grape fruit (grape peel, pulp, grape seed).

The extract is fermented pulverized grape fruit (grape peel, pulp and grape seeds) at 20-25 ℃ for 6-8 days, and then the filtrate is filtered and aged under constant temperature at 11 ℃ for 30 days and concentrated under reduced pressure It is carried out by spray drying. At this time, it is effective to perform vacuum concentration up to 40-45 weight% with a vacuum evaporator. In addition, a blender is used to grind the grape fruit. Performing the fermentation for 6-8 days at 20-25 ° C is related to the fermentation capacity of the natural yeast on the skin, and if the temperature is below 20 ° C, the fermentation period becomes too long. There is a risk of interruption. When fermentation is carried out at the temperature for 6 to 8 days, all the sugars disappear and the fermentation is completed. Once fermentation is complete, avoid direct sunlight and continuously aged at 11 ° C. for 30 days under unchanged temperature. This is because, when the aged wine is exposed to direct sunlight, the wine, which is easily oxidized and is called living organic matter, changes well according to time, temperature, and light. In other words, if the temperature is too low, the aging does not work properly. If the temperature is too high, it is not suitable for aging for a long time. Therefore, in order to extract the active ingredient, the aging temperature is preferably 11 ° C., and the period is preferably aged for 30 days. . Through this aging step, many active ingredients are eluted.

Aged extract is concentrated under reduced pressure until the extract is about 40 to 45%, in order to shorten the process time when using a spray dryer.

Concentration is preferably performed under reduced pressure of 25 to 35 CmHg. When the concentration is less than 25CmHg, the concentration time is long, and when it is 35CmHg, a large amount of active ingredient is discharged.

The final extract obtained by vacuum concentrated extract using a spray dryer (In let: 150 ~ 200 ℃, Out let: 80 ~ 100 ℃) is a powdered grape fermentation extract. By using the spray dryer, it is possible to remove the alcohol component that can cause skin irritation, and to concentrate the active ingredient having the whitening effect and the anti wrinkle effect. Table 1

Grape fermentation extract according to the present invention is excellent in whitening and anti-wrinkle effect.

Therefore, it can be added to all cosmetics related to whitening and anti-wrinkle, such as supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, face powder, foundation, etc. The amount is 0.01 to 10% by weight based on the total weight of the cosmetic composition It is preferable to set it as about 0.1 to 5 weight% preferably. When the concentration is less than 0.01% by weight, it is difficult to obtain the whitening and anti-wrinkle effect, and when it is blended at 10% by weight or more, the increase of the whitening and anti-wrinkle effect is insignificant compared to the amount used, and it is uneconomical in terms of stability of the product.

The preparation method of the grape fermentation broth extract according to the present invention is described in the following examples. However, the following examples are intended to illustrate the present invention, but the scope of the present invention is not limited thereto.

Example 1

Crush 5 kg of grape fruit (mask bailey, grape skin, pulp, grape seed) into a brewing container, ferment at 22 ° C for 6 days, filter with 300 mesh filter cloth, and continuously keep the temperature unchanged. It was aged for 30 days at 11 ℃. The mature product (3,750 ml) was concentrated under reduced pressure until concentrated to 40-45% with a Tokyo Rikakikai Co., Rotary vacuum evaporator, and the powdered grape fermentation extract was extracted using a spray dryer (model MH-8). 10 g was obtained.

Example 2

After crushing 5kg of grape fruit (campbell) (grape peel, flesh, grape seed), squeezed grape juice is placed in a brewing vessel and fermented at 20 ° C for 8 days, and then filtered with 300 mesh filter cloth (Cheongyesang). It was aged for 30 days at 11 ℃ continuously without changing.

The matured product (4,000 ml) was concentrated under reduced pressure until concentrated to 40-45% with a Tokyo Rikakikai co, Rotary vacuum evaporator, and 12 g of powdered grape fermentation extract was extracted using a spray dryer (model MH-8). Got.

Comparative Example 1

5 kg of grape fruit (campbell) (grape peel, pulp, grape seed) was crushed and the extracted extract was filtered using a 300 mesh filter cloth (Cheongye Sangsa).

The extract was concentrated under reduced pressure using a Tokyo Rikakikai co, Rotary vacuum evaporator to obtain 500 g of a viscous grape extract.

Comparative Example 2

5 kg of grape fruit (mask bailey) (grape peel, pulp, grape seed) was crushed and extracted with 2 L of ethanol in a water bath (18-23 ° C.) for 24 hours, followed by filtration using a 300 mesh filter cloth (Cheongye Co., Ltd.). The extract was concentrated under reduced pressure until concentrated to 40-45% with a Tokyo Rikakikai co, Rotary vacuum evaporator, and 13.5 g of powdered grape extract was obtained using a spray dryer (model MH-8).

Preparation Example 1 (Flexible Cosmetic Water)

The aqueous phase (raw materials 1 to 4) and the ethanol phase (raw materials 5 to 7) were separated and weighed according to the following composition, and then dissolved separately, and the ethanol phase was put into the aqueous phase and mixed. Subsequently, additives (raw materials 8-9) were added and mixed. A flexible lotion was prepared by filtration using these membrane filters (Samdeok Filtration Co., Ltd., paper filter).

division Raw material name weight(%) One Purified water 88.320 2 Grape Fermentation Extract of Example 1 0.100 3 Butylene glycol 5.000 4 glycerin 1.000 5 Modified ethanol 5.000 6 Paraoxyethylene Cured Castor Oil 0.300 7 Paraoxybenzoic Acid Methyl 0.100 8 Red 401 (0.2%) 0.085 9 Spices (E-5177) 0.095

Preparation Example 2 (Nutrition Cosmetics)

The water phase (raw materials 1-6) and the oil phase (raw materials 7-11) were separately weighed according to the following composition, and then mixed and dissolved separately at 80 ± 2 ° C., and the oil phases were mixed with water. Subsequently, the raw material 12 which is a neutralizing agent was added and neutralized, and it cooled to 50 degreeC. After cooling, the raw material 13 was added and then cooled to 35 ° C. to prepare a nourishing lotion.

division Raw material name weight(%) One Purified water 79.905 2 Grape Fermentation Extract of Example 1 0.100 3 glycerin 5.000 4 Methylgluses 20 (manufactured by Amerchol) 1.500 5 Carboxy Vinyl Polymer 0.200 6 Paraoxybenzoic Acid Methyl 0.100 7 Liquid paraffin 10.000 8 Self-emulsifying monoglycerate glycerin 1.300 9 Stearic acid 0.700 10 Monostearine polyoxyethylene sorbitan (20E.O) 0.600 11 Cetanol 0.300 12 Triethanolamine 0.200 13 Spices (E-5177) (Hanbul Farming) 0.095

Preparation Example 3 (Nutrition Cream)

According to the following composition was prepared in the same manner as in Preparation Example 2.

division Raw material name weight(%) One Purified water 78.755 2 Grape Fermentation Extract of Example 1 0.100 3 glycerin 4.000 4 Methylgluses 20 (manufactured by Amerchol) 2.000 5 Carboxy Vinyl Polymer 0.150 6 Paraoxybenzoic Acid Methyl 0.100 7 Liquid paraffin 7.000 8 Self-emulsifying monoglycerate glycerin 1.000 9 Stearic acid 3.000 10 Monostearine polyoxyethylene sorbitan (20E.O) 1.500 11 Cetanol 2.000 12 Triethanolamine 0.300 13 Spices (E-5177) 0.095

Preparation Example 4 (Essence)

According to the following composition, the raw materials were sequentially measured, mixed and dissolved, and then filtered through a 200 mesh sieve to prepare an essence.

division Raw material name weight(%) One Grape Fermentation Extract of Example 1 0.500 2 glycerin 5.000 3 Butylene glycol 3.000 4 Spices (E-5177) 0.095 5 Rose water 91.405

Preparation Example 5 (two-way cake)

A (raw material 1-9) and b (raw material 10-11) were fully mixed in the mixer according to the following composition. C. (Raw 12-13) was heated (80 ° C) and then a) and b) mixed at high speed. Two-way cake was manufactured by molding with a molding machine (Interpack Korea Co., HYD-Pressing).

division Raw material name weight(%) One Mica 20.500 2 kaoline 15.000 3 Titanium dioxide 12.000 4 Barium sulfate 3.000 end 5 Zinc stearate 2.000 6 Calcium silicate 2.000 7 Imidazolidinylurea 0.250 8 Paraoxybenzoic Acid Methyl 0.020 9 Silica 0.010 I 10 Talc 36.120 11 Grape Fermentation Extract of Example 1 3.000 All 12 Methyl Polysiloxane 6.000 13 Octylmethoxycinnamate 0.100

Comparative Preparation Examples 1 to 5

Except for using the grape extract of Comparative Example 2 instead of the grape fermentation extract of Example 1 were prepared in the same composition and the same method as Preparation Examples 1 to 5, respectively.

Experimental Example 1

With respect to the grape fermentation broth extract obtained in Examples 1 and 2 and the grape extract obtained in Comparative Examples 1 and 2, the active oxygen scavenging ability (active oxygen species cause various adult diseases and aging, in particular skin aging, elimination Capacity is essential for functional anti-wrinkle cosmetics.) The content of catechin, a substance with), was measured. Grape fermentation extracts obtained in Examples 1 and 2, grape extracts obtained in Comparative Examples 1 and 2, catechin standards (Sigma) about 5mg each weighed precisely, each dissolved in 50ml of the mobile phase, and then put the mobile phase again 100ml And standard solution.

With the sample solution and the standard solution 20㎕ using liquid chromatography (1100Series, AT G) is obtained, and _T Q Qs of catechins for the peak area of material.

The amount of catechin as polyphenol was calculated | required by the following formula.

Amount of catechin (mg.) As polyphenol = amount of catechin standard (mg) ×

The operating conditions of the liquid chromatography for the test are as follows.

Mobile phase: Dissolve 1.0 g of phosphoric acid in 50% methanol solution and make 2 L.

Detector: UV absorbance photometer (wavelength 280nm)

Column: C ₁₈

Flow rate: 1.0ml / min

The test results are shown in Table 1 below.

sample Number of tests Catechin content (%) as polyphenol Average Grape Fermentation Extract of Example 1 One 95.0 94.9 2 94.8 3 94.9 Grape Fermentation Extract of Example 2 One 85.2 85.3 2 84.8 3 85.9 Grape extract of Comparative Example 1 One 30.5 31.3 2 31.6 3 31.9 Grape extract of Comparative Example 2 One 5.2 5.6 2 5.8 3 5.9

As shown in Table 1, the catechin content excellent in the active oxygen scavenging ability in the grape fermentation extract obtained in Example 1 was found to be significantly higher.

Product Comparison in Experimental Example 1

The catechin content of the cosmetics of Preparation Example 1 and Comparative Preparation Example 1 was measured and compared.

Grape fermentation extract of Example 1 for Preparation Example 1 and 1g of the amount corresponding to 1mg of the grape extract of Comparative Example 2 for Comparative Preparation Example 1 was precisely weighed, and 50 ml of the test mobile phase was added thereto as a sample solution. Separately, 10 mg of the catechin standard was precisely weighed, 100 ml of the test mobile phase was added thereto, and 10 ml of the test mobile phase was added thereto.

The catechin content in the cosmetic was determined using liquid chromatography (1100 series, AT) with the sample solution and the standard solution. Operation conditions of the liquid chromatography is the same as in Experiment 1.

sample Catechin content in cosmetics (%) Preparation Example 1 90.5 Comparative Production Example 1 4.2

As shown in the table, it was confirmed that Preparation Example 1 using the grape fermentation extract of Example 1 exhibited an excellent catechin content than the cosmetic preparation of Comparative Preparation Example 1 using the grape extract of Comparative Example 2.

Experimental Example 2

The effect of inhibiting the tyrosinase activity of the grape fermentation broth extract obtained in Examples 1 and 2, the grape extract obtained in Comparative Examples 1 and 2, ascorbic acid and arbutin was measured. Enzyme tyrosinase was isolated and purified from Mushroom (Mushroom) was used Sigma. First, L-tyrosine (manufactured by Sigma), which is a substrate, was made into a 0.3 mg / ml solution in distilled water, and 1.0 ml of this solution was taken into a test tube. Here, 1.0 ml of potassium phosphate buffer (potassium phosphate buffer: pH 6.8, 0.1 M) and the grape fermentation broth extract obtained in Examples 1 and 2, grape extracts obtained in Comparative Examples 1 and 2, ascorbic acid (manufactured by Sigma), 0.7 ml of a diluting solution (1-> 10) of arbutin (copperizing agent) was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostat. The diluting solvent was made into a total amount of 50ml by using water, and as a control, 0.7 ml of the diluting solvent was used instead of the grape fermentation broth extract obtained in Examples 1 and 2, the grape extract obtained in Comparative Examples 1 and 2, ascorbic acid and arbutin. 0.1 ml of tyrosinase (1.25unit / ml) was added to this reaction solution, and it was made to react in 37 degreeC thermostat for 10 minutes. The test tube containing the reaction solution was placed in iced water, quenched to stop the reaction, and the absorbance was measured at a wavelength of 475 nm with an absorbance spectrometer (160-A, Shimazu).

The tyrosinase activity inhibitory effect was calculated | required by the following formula.

Tyrosinase Inhibition Rate (%) = 100- × 100

From the tyrosinase inhibition rate of the dilution solution of each sample obtained by the above formula, the dose of each sample (IC ₅₀ = Inhibition Concentration 50%) that reduced the tyrosinase activity by 50% was determined.

The test results are shown in Table 2 below.

sample IC ₅₀ Grape fermentation extract of Example 1 0.039 Grape Fermentation Extract of Example 2 0.051 Grape extract of Comparative Example 1 0.197 Grape extract of Comparative Example 2 0.321 Arbutin 0.190 Ascorbin Mountain 0.180 IC ₅₀ (Inhibition Concentration 50%): Dose of each sample that inhibits tyrosinase formation by 50% in the reaction system.

As shown in Table 2, the grape fermentation extract obtained in Examples 1 to 2 has a very strong tyrosinase activity inhibitory effect compared to the grape extracts, ascorbic acid, arbutin obtained in Comparative Examples 1 and 2, accordingly It could be confirmed that it has an excellent whitening effect.

Experimental Example 3

Whitening effect

In order to test the whitening efficacy of the cosmetic of the present invention, four sites of 2 × 2 cm ² were set in the upper right arm of the test subject (30 healthy male and female volunteers). An aluminum foil was placed on the arm so that ultraviolet light was irradiated only on the site under test, and 3 times consecutively at 0.8 × 10erg ⁷ / cm ³ / times / day using a solar simulator multiport (modelXPS400) manufactured by solar light at a distance of 10 cm. Irradiation was carried out. Prior to irradiation, the area under test was well cleaned with hot water. After the irradiation, the cream of the same composition, except that Preparation Example 3 and Preparation Example 5 containing the grape fermentation broth extract of Example 1 in such a portion, and did not contain the grape fermentation extract of Preparation Example 3, Comparative Example 2 Comparative Preparation Example 3 containing the grape extract was applied as a control, three times a day for three weeks. Each treatment site was measured for 3 weeks using a color difference meter (CR300 Siries) of Minolta, Japan. The results are shown in FIG. Fig. 1 shows the L value (brightness) after ultraviolet irradiation of 30 people using a color difference meter, and after the measurement is completed, the respective manufacturing examples are applied to the irradiated site to measure the brightness values. The left bar is the average value of L values for 30 people after UV irradiation, and the right bar is the brightness value obtained by using each preparation for 3 weeks after obtaining the average value. The greater the brightness value, the greater the whitening effect.

As shown in FIG. 1, the cosmetic composition containing the grape fermentation extract of the present invention is whitening superior to the cosmetic composition containing no grape fermentation extract and the cosmetic composition of Comparative Preparation Example 3 containing the grape extract of Comparative Example 2. Efficacy was shown.

Experimental Example 4

To 20 test subjects with facial wrinkles aged 30 to 40 years, the cosmetic composition of Preparation Example 3 having the same composition was prepared except that Preparation Example 3 containing the grape fermentation extract of Example 1 and Grape fermentation extract were not contained. The skin wrinkle improvement effect was compared and evaluated. Preparation Example 3 containing the grape fermentation extract of Example 1 on the left side of the subject, except that the grape fermentation extract on the right side did not contain Preparation Example 3 twice a day (morning, evening) 60 It was used for days. The skin on both sides of the face before the use of the cosmetic was measured and the same area was remeasured 60 days after the use of the cosmetic to measure the amount of skin wrinkle change.

Skin wrinkles were measured in a constant temperature and humidity room with a temperature of 24 ° C and a relative humidity of 40%. The wrinkles at the crowns were made using silicone rubber (silflo) to make replicas, and skin visometer (courage + Khazaka) G) was used for image analysis.

As a result of the measurement, except that the grape fermentation extract of Example 1 was not contained, the average wrinkle depth of the site using Preparation Example 3 of the same composition was decreased from 38 μm to 35 μm and the fermentation extract of Example 1 was contained. Wrinkle average depth of the site using the Preparation Example 3 was reduced from 40 ㎛ to 24 ㎛ showed a very good skin wrinkle improvement effect.

In addition, the cosmetics of Preparation Example 3 containing the grape fermentation extract of Example 1 and Comparative Preparation Example 3 containing the grape extract of Comparative Example 2 were tested on 20 other subjects with facial wrinkles aged 30 to 40 years. The skin wrinkle improvement effect was compared and evaluated. In the face seat of the examinee, Preparation Example 3 containing the grape fermentation extract of Example 1, and Comparative Preparation Example 3 containing the grape extract of Comparative Example 2 were used twice a day (morning and evening) for 60 days. It was. The skin of both sides of the face before the use of the cosmetic was measured and the same area was remeasured 60 days after the use of the cosmetic to measure the amount of skin wrinkle change. Skin wrinkles were measured in a constant temperature and humidity room with a temperature of 24 ° C and a relative humidity of 40%. Wrinkles on the crowfeet were made of silicone rubber (silflo) to make a replica, and a skin viscometer (Skinvisometer; Courage + Khazaka) Image analysis was performed.

As a result, the wrinkle average depth of the site using Comparative Preparation Example 3 containing the grape extract of Comparative Example 2 was decreased from 39 μm to 35 μm, and the site using the Preparation Example 3 containing the grape fermentation extract of Example 1 was used. Wrinkle average depth was reduced from 41 ㎛ to 23 ㎛ showed a very good skin wrinkle improvement effect.

Experimental Example 5

With the grape fermentation broth extract obtained in Examples 1 and 2 of the present invention and the grape extracts obtained in Comparative Examples 1 and 2, each sample was applied to a pin chamber (manufactured by Epit Ltd. oy) for 20 adult men and women After 24 hours of attachment to the inner soft skin, skin conditions such as skin irritation, erythema, itching, and allergy were observed, and again after 24 hours.

Number of skin protons / total subjects for sample sample 24 hours later 48 hours later Grape fermentation broth extract obtained in Example 1 0/20 0/20 Grape fermentation broth extract obtained in Example 2 0/20 0/20 Grape extract obtained in Comparative Example 1 0/20 0/20 Grape extract obtained in Comparative Example 2 5/20 5/20

As shown in Table 3, the skin irritation occurred in Comparative Example 2 using the organic solvent, it could be confirmed that there is a problem in safety.

As described above, according to the present invention, it is possible to obtain a grape fermentation broth extract having a tyrosinase inhibitory effect, that is, a whitening effect, far superior to that of arbutin, ascorbic acid, and the like, which are previously developed, and have a whitening effect, anti-wrinkle effect and stability. A cosmetic composition containing grape fermentation broth extract excellent in terms of color can be obtained.

Claims (2)

Whitening and anti-wrinkle cosmetic composition characterized in that the powdered grape fermentation broth extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition. According to claim 1, wherein the grape fermentation extract of grape skin, flesh and grape seed pulverized fermentation for 6 to 8 days at 20-25 ℃, the filtrate is filtered and aged for 30 days continuously at a constant temperature of 11 ℃ Whitening and anti-wrinkle cosmetic composition, characterized in that obtained by concentration under reduced pressure and spray drying.