KR20140089305A - Composition for improving skin whitening or skin wrinkle comprising extracts of Quercus salicina Blume - Google Patents

Abstract

Provided by the present invention are a cosmetic composition for whitening skin or preventing or alleviating wrinkles, a pharmaceutical composition, and a composition for health food, which include Quercus salicina Blume as an active component. The composition according to the present invention is able to solve problems of various side effects of conventional substances which efficiently whiten skin or alleviate wrinkles by using a plant medicine which is approved for safety as being used for a long time.

Description

TECHNICAL FIELD The present invention relates to a skin whitening or skin wrinkle containing extracts of Quercus salicina Blume containing a wood extract as an active ingredient,

The present invention relates to a composition for preventing or improving skin whitening or wrinkles, and more particularly, to a cosmetic composition, a pharmaceutical composition, and a cosmetic composition for preventing or improving whitening or wrinkling of skin containing plant herbal medicine or extract thereof as an active ingredient And a composition for health functional foods.

Skin is the primary barrier of the human body. It protects the internal organs of the body from external stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. This important skin also becomes aged gradually as the age of the human body grows, and as a result, skin elasticity loss, keratinization, wrinkle formation, skin atrophy and the like appear. Such skin aging phenomenon not only causes wrinkles on the skin surface but also causes problems such as pigment deposition.

The appearance of wrinkles on the skin is a phenomenon accompanied by aging, which shows various structural changes due to the deterioration of vital function. That is, it is classified into intrinsic aging and aging caused by an increase in age, and photoaging due to the addition of external factors, particularly ultraviolet damage. There are many kinds of outsiders, but they are most affected by ultraviolet rays in sunlight. In the endogenous aging process, characteristic clinical skin findings include fine wrinkles, atrophy of the dermis, and reduction of the subcutaneous fat layer. In the photoaging process, rough and deep wrinkles appear and abnormal elastic fibrous material accumulates and the skin becomes thick and loosens like leather .

Much research has been carried out on the importance and role of collagen directly affecting such wrinkles and skin elasticity. When the majority of the skin, 77% of the skin, and 90% of the dermis are rich in quantity and quality of collagen, the skin can also be healthy and beautiful without wrinkles and sagging. Collagen is sometimes incorporated into cosmetics and commercialized. When collagen is applied to the skin surface as a cosmetic product, it is difficult to absorb the transdermal collagen, which is a polymer, so that its function can not be sufficiently expected. In addition, there was a method of injecting collagen directly into the dermis of the skin, but this method has not been a solution for improving the wrinkles of the skin due to side effects.

There are many factors involved in determining skin color, the most important being melanin pigment. The melanin pigment is produced by the action of the tyrosine ethylacinase enzyme in the melanocytes present in the basal layer of the epidermis. Tyrosine is oxidized by tyrosinase to produce waveguide and ultimately melanin pigment is produced. The melanin pigment is also produced by skin inflammation, endocrine abnormality, neurotic factors, etc., and the action of melanocyte is strengthened by ultraviolet rays. Melanin, which is the cause of abnormal pigmentation of the skin including stains, is produced by the oxidation reaction of tyrosine with tyrosinase catalysis, but when the highly reactive reactive oxygen is present, the melanin production is rapidly increased. Furthermore, the increase of active oxygen damages the collagen fibers, which causes wrinkles. It is here that 80% of the causes of wrinkles are due to ultraviolet rays.

Recently, as the leisure population has increased, more people are enjoying their activities outside, and more interest in their appearance than in the past has increased the demand for prevention of skin pigmentation and aging caused by ultraviolet rays. Accordingly, substances having an activity of inhibiting ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase have been used in combination with cosmetics or medicines. However, their inadequate whitening effect, Its use is limited due to its weakness and safety to the skin. Therefore, there is a need to develop compositions that are more effective and safe for improving wrinkles and whitening.

On the other hand, the tree ( Quercus salicina Blume) is a plant belonging to the oak tree and is distributed in Jeju Island. When participating, the tree has been used as a natural medicinal substance to dissolve stones in the body without side effects or toxicity.

In addition, Cho et al. Reported on the anti-inflammatory effect of wood upon participation (Non-Patent Document 1).

However, the prevention, improvement, or therapeutic effect of skin aging including whitening and wrinkling of trees at the time of participation is not known at all.

1.Cho Kyung Hee et al. Korean Journal of Oriental Physiology and Pathology, 2011, 25 (3) .540-545

Accordingly, the inventors of the present invention have conducted research on numerous herbal medicines in order to discover herbal medicines effective for effective and safe skin whitening and improvement, and have completed the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition for skin whitening or skin wrinkle improvement containing plant herbal medicine.

Another object of the present invention is to provide a pharmaceutical composition for preventing or improving skin whitening or skin wrinkles including plant herbal medicine.

It is still another object of the present invention to provide a composition for health functional foods for preventing or improving skin whitening or skin wrinkles containing plant herbal medicine.

In order to attain the above object, one aspect of the present invention provides a cosmetic composition for preventing or improving whitening or wrinkling of skin comprising wood ( Quercus salicina Blume) as an active ingredient.

Another aspect of the present invention provides a pharmaceutical composition for prevention or improvement of whitening or wrinkling of skin comprising wood as an active ingredient upon participating.

Another aspect of the present invention provides a composition for a health functional food for preventing or improving whitening or wrinkling of skin comprising wood as an active ingredient at the time of participation.

Hereinafter, the present invention will be described in more detail.

All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference.

The inventors of the present invention have studied to discover herbal medicines effective for whitening or wrinkling of the skin from a large number of plant herbal medicines. As a result, it has been found that the wood extract has a very high activity for skin whitening and wrinkle improvement. Specifically, the wood extract showed a very high antioxidative activity as a result of measuring DPPH decolorization-induced antioxidative activity, and it was expected to be effective for skin whitening (Experimental Example 1). In addition, the degree of suppression of MMP-1, which promotes collagen degradation, was significantly (p <0.05) significantly inhibited by MMP-1 inhibition (Example 2), and dermal fibroblasts were cultured, As a result of the experiment for promoting the production, it was confirmed that there was a significant promoting effect on the production of procollagen (Experimental Example 3), and it was confirmed that it is effective for preventing, improving and treating wrinkles of the skin.

Accordingly, in one aspect, the present invention provides a cosmetic composition for preventing or improving whitening or wrinkling of skin comprising wood ( Quercus salicina Blume) as an active ingredient.

In another aspect, the present invention provides a pharmaceutical composition for preventing or improving whitening or wrinkling of skin comprising wood as an active ingredient at the time of participation.

In another aspect, the present invention provides a composition for health functional food for preventing or improving whitening or wrinkling of skin comprising wood as an active ingredient at the time of participation.

In the present invention, the term " health functional food "means a food prepared and processed by using a raw material or ingredient having useful functionality in the human body according to the Health Functional Food No. 3727, and" And function of the nutrient for the purpose of obtaining a beneficial effect in health use such as controlling the nutrient or physiological action.

The active ingredient of the composition may be contained in the form of an extract, or may be contained as a pulverized product of the herbal medicine itself or as a dried pulverized product of the herbal medicine.

Wherein the extract of the participating tree is an extract of a solvent selected from the group consisting of water, C ₁ -C ₄ alcohol, acetone, ethyl acetate, n-butanol, chloroform, methylene chloride and combinations thereof, It may be a more purified fraction. The extract of the tree at the time of participation may preferably be an extract of water, a C ₁ -C ₄ alcohol, or a combination thereof, but most preferably it can be contained as an extract of 70 to 90% aqueous ethanol solution. The% is the concentration by volume percentage (v / v) and refers to a volume of solute dissolved in a volume of solution as is well known in the art. For example, 30% (v / v) means that 30 mL of solute is dissolved in 100 mL of solution.

When extracting with solvent, the extraction includes both the method of immersing the tree at the time of participation and the method of distilling the tree as the extraction solvent at the time of participation. Any method such as cold-rolling, heating, ultrasonic wave, and refluxing may be applied to the extraction method by immersion. The extraction time may be 1 day or more, preferably 3 days or more, and may be varied depending on conditions such as extraction solvent and extraction temperature.

The extraction may be performed one or more times several times to obtain a larger amount of the active ingredient, and an extract obtained by adding the respective extracts obtained by extracting several times may be used.

The extract extracted by the above-described method may be used as it is, or may be used in the form of concentrated X-ray. Preferably, it is used as a concentrated form so that it can be used at a sufficient concentration. The concentration can be achieved by concentrating the extract at a reduced pressure, for example, at a temperature of 30 ° C. It may be concentrated and then lyophilized to be used as a lyophilized product.

As used herein, the term "whitening" is understood as a result of inhibition of the production of melanin or promotion of degradation of melanin, specifically, as a result of prevention or suppression of symptoms resulting from an increase of melanin. Such symptoms include, but are not limited to, spots, freckles, skin aging, etc., and should be understood as all the symptoms leading to skin whitening by preventing or suppressing the symptoms resulting from the increase of melanin.

The cosmetic composition according to one aspect of the present invention may be manufactured into a specific formulation by a method widely known in the field of cosmetics, and may further include a cosmetically acceptable additive. Such additives include stabilizers, solubilizers, emulsifiers, vitamins, pigments, fragrances, and combinations thereof, but the present invention is not limited thereto, and may vary depending on the type of formulation to be prepared.

The cosmetic compositions of the present invention may be prepared in any formulation conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, surfactant-containing cleansing, liquid foundations, Cream foundation, and the like, but the present invention is not limited thereto. More specifically, it can be manufactured by a formulation such as a soft lotion, a nutritional lotion, a nutritional liquid, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water,

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, carboxyl vinyl polymer, propylene glycol, 1,3-butylene glycol, Esters, polyethylene hardened castor oil, preservatives or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, the carrier component may include water, a flavoring agent, a liquid diluent such as concentrated glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan Emulsifying suspending agents such as esters, microcrystalline cellulose, carboxyvinyl polymer, thickener or preservative can be used.

In the case where the formulation of the present invention is a surfactant-containing cleansing, in the case of the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether An alkylamidobetaine, an aliphatic alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a lanolin derivative, or an ethoxylated glycerol fatty acid ester.

The pharmaceutical composition according to another aspect of the present invention may be formulated into a conventional pharmaceutical formulation known in the art. The pharmaceutical composition may be formulated into any formulation including, but not limited to, an oral administration preparation, an injectable preparation, a suppository, a transdermal preparation, an external preparation, and a parenteral preparation, , Granules, tablets, capsules, pills, or expectorants, for oral administration.

When formulating into each of the above-mentioned formulations, it may be prepared by adding a pharmaceutically acceptable carrier or an additive necessary for the preparation of each of the formulations. Representative examples of the formulations for oral administration include at least one selected from the group consisting of diluents, lubricants, binders, disintegrants, sweeteners, stabilizers, and preservatives as the carrier when formulated. Examples of the additives include perfumes, vitamins, and antioxidants May be selected and used.

Examples of the diluent include lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol as the diluent, magnesium stearate or talc as the lubricant, polyvinyl pyrrolidone Or hydroxypropylcellulose are preferable. Examples of the disintegrant include carboxymethylcellulose calcium, sodium starch glycolate, polacrilin potassium, or crospovidone; examples of sweeteners include white sugar, fructose, sorbitol, or aspartame; stabilizers include sodium carboxymethylcellulose, Preferred examples of the antiseptic agent include methyl paraoxybenzoate, propyl paraoxybenzoate, and potassium sorbate.

In addition to the above components, natural additives such as natural flavors such as plum flavor, lemon flavor, pineapple flavor and herb flavor, natural fruit juice, natural coloring matters such as chlorophyllin and flavonoid, fructose, honey, sugar alcohol, sugar Or acidic agents such as citric acid and sodium citrate may be mixed and used.

In order to obtain the effect of preventing and improving whitening and wrinkling of the skin, the pharmaceutical composition is preferably formulated so that the total daily dose as an active ingredient is 0.1 to 0.8 mg / kg as an extract and 10 to 80 mg / kg as a herbal medicine It may be administered in several divided doses. The dose may be suitably increased or decreased according to the degree of skin whitening and wrinkling, age, oily and dry skin, sex, and the like.

The composition for a health functional food according to another aspect of the present invention may be formulated into a conventional health functional food formulation known in the art. The health functional food may be prepared, for example, as a powder, a granule, a tablet, a capsule, a suspension, an emulsion, a syrup, a liquid, an extract, tea, jelly, As such a pharmaceutically acceptable carrier or additive, any carrier or additive known to be usable in the art for the preparation of the formulation to be prepared may be used.

When the health functional food is a beverage, it may further contain various flavors or natural carbohydrates as well as ordinary beverages. Such natural carbohydrates include, but are not limited to, glucose, monosaccharides such as fructose, disaccharides such as maltose, sucrose, and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol and erythritol.

Since the tree has been used as a medicinal material or tea for a long time and the tree extract derived from the tree has no toxicity or side effect problems, the cosmetic composition, the pharmaceutical composition and the composition for health functional food according to the present invention There is an advantage that it can be used safely even when used for a long time in order to prevent or improve whitening or wrinkles of the skin.

As described above, the extract of wood according to the present invention has an effect of improving whitening and wrinkling of the skin, so that the cosmetic composition, the pharmaceutical composition, or the health functional food composition for effectively preventing or improving whitening or wrinkling of the skin Can be used as an active ingredient.

In addition, the composition according to the present invention has an advantage of being able to solve the problems of various side effects that a substance effective for whitening and a substance for improving wrinkle, which have been proven to be safe, have been used as raw materials for a long period of time .

FIG. 1A is a graph showing the results of measurement of DPPH radical scavenging effect of ascorbic acid as a positive control group. FIG.
FIG. 1B is a graph showing the results of measuring DPPH radical scavenging effect of wood extracts at the time of entry according to one embodiment of the present invention.
FIG. 2 is a graph showing the results of MMP-1 assay of wood extracts at the time of entry according to an embodiment of the present invention. FIG.
FIG. 3 is a graph showing the results of performing a procollagen assay of a tree extract at the time of entry according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these embodiments are provided to aid understanding of the present invention, and the scope of the present invention is not limited thereto in any sense.

Example 1: Preparation of tree extracts at the time of participation

(Quercus salicina Blume) leaves were dried and then subjected to extraction by hot water extraction for about 2 hours with 50% (v / v) ethanol of 15 times of the dry weight of the sample and then the supernatant was removed. The solvent of the pear was added and re-extracted. The extracted solutions were combined and filtered using a Whatman filter paper (No. 5). The filtrate was concentrated at 40 ° C using a vacuum concentrator to obtain a solid form of the extract.

Experimental Example 1: Antioxidant activity measurement

1,1-Diphenyl-2-picrylhydrazyl (DPPH) is a very stable free radical, and its antioxidant activity can be easily visualized by decolorizing by proton-radical scavenger among various antioxidant mechanisms. Using this principle, antioxidative activity using DPPH was measured.

The extract obtained in Example 1 was diluted in DMSO to prepare samples at various concentrations, and the DPPH radical scavenging effect was measured. The same test was performed on ascorbic acid as a positive control and compared.

The results are shown in Figs. 1A and 1B.

FIG. 1A is a graph showing the results of measurement of DPPH radical scavenging effect of ascorbic acid as a positive control group. FIG.

FIG. 1B is a graph showing the results of measuring DPPH radical scavenging effect of wood extracts at the time of entry according to an embodiment of the present invention.

According to the results shown in Figs. 1A and 1B, the wood extract showed DPPH radical scavenging effect similar to that of ascorbic acid, which is a positive control.

Experimental Example 2: MPP-1 Assay

(Matrix metalloproteinase-1), which promotes the degradation of collagen, was performed. First HDF-N (Human Dermal Fibroblast- neonate) cells FBS (fetal bovine serum) containing a DMEM (Dulbecco's Modified Eagle Medium) in 24 well plate in culture medium was inoculated so that the ^{2X10 4 cells / well, 5%} CO 2 , And cultured at 37 ° C for 24 hours. After 24 hours of incubation, the medium was removed and washed with DPBS (Dulbecco's Phosphate Buffered Saline). 200 μl of DPBS was added and irradiated with ultraviolet UV-A at 5 J / cm ² . DPBS was discarded and the samples were treated at different concentrations and cultured at 37 ° C for 48 hours in 5% CO ₂ . After 48 hours, the MMP-1 contained in the medium was measured by an immuno ELISA assay (R & D system), and the cells attached to the plate were lysed with 1N NaOH and quantified with MMP-1 protein. The measured MMP-1 was calibrated with the amount of protein. The tree extracts used in the above-described Example 1 were used as a tree extract, and 1 μM of retinoic acid was used as a positive control.

The results are shown in Fig.

FIG. 2 is a graph showing the results of MMP-1 assay of wood extracts at the time of entry according to an embodiment of the present invention. FIG.

2, it can be seen that MMP-1 is significantly increased in the group irradiated with ultraviolet light (+) compared to the group not irradiated with ultraviolet light (-), and the increase of MMP- The extracts of the wood extracts showed a significant reduction of MMP-1 compared to the control group, which is a retinic acid treatment group.

Experimental Example 3: Proclara assay

Experiments were conducted to measure the promoting effect of procollagen production.

First, HDF (human dermal fibroblast) cells were inoculated in an 8-well plate at 1.4 × 10 ⁴ cells / well and cultured. After starvation for 24 hours, treatment was carried out as follows.

- Positive control: TGF-beta 10 ng / ml

- Samples: At the time of participating in Example 1, 0.1, 0.5, 1 ppm

After culturing for 24 hours, the cell culture medium was collected and the content of procollagen was measured. TAKARA MK101 procollagen ELISA kit was used for the measurement of procollagen content. The measured procollagen concentration was corrected to the total protein concentration. The results are shown in Fig.

FIG. 3 is a graph showing the results of performing a procollagen assay of a tree extract at the time of entry according to an embodiment of the present invention.

According to Fig. 3, the extracts of trees showed a significant effect of promoting the production of procollagen.

Claims (10)

A cosmetic composition for preventing or improving the whitening or wrinkling of the skin, which comprises wood ( Quercus salicina Blume) as an active ingredient at the time of participation. A pharmaceutical composition for preventing or improving the whitening or wrinkling of skin, which comprises wood as an active ingredient upon its participation. A composition for a health food for preventing or improving whitening or wrinkling of the skin, which comprises wood as an active ingredient upon its participation. 4. The composition according to any one of claims 1 to 3, wherein the tree at the time of participating is contained as a pulverized product of the herbal medicine itself, a dried pulverized product of herbal medicine, or an extract. 5. The composition of claim 4, wherein the extract of the participant tree is an extract of a solvent selected from the group consisting of water, C ₁ -C ₄ alcohol, acetone, ethyl acetate, n-butanol, chloroform, methylene chloride and combinations thereof. 6. The composition of claim 5, wherein the wood extract is 70-90% ethanol aqueous solution. 4. The composition according to any one of claims 1 to 3, wherein the whitening is for removing or improving spots, freckles, or spots. The cosmetic composition according to claim 1, wherein the cosmetic composition has a formulation of a liquid, suspension, emulsion, paste, gel, cream, lotion, surfactant-containing cleansing, liquid foundation or cream foundation. The pharmaceutical composition according to claim 2, wherein the pharmaceutical composition has a formulation of an acid, a granule, a tablet, a capsule, a suspension, an emulsion, a syrup, a solution, an aerosol, an excision, an injection, a transdermal preparation or a suppository. The composition for a health functional food according to claim 3, wherein the composition for health functional foods is a composition for health functional foods having formulations of powders, granules, tablets, capsules, suspensions, emulsions, syrups, solutions, aerosols, extracts, tea, jellies, .

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