KR102186798B1 - Antibacterial and whitening-functional composition containing Quercus Acutissima Sap - Google Patents

Abstract

The present invention relates to an antibacterial and whitening functional composition containing Quercus acutissima sap. The antibacterial and whitening functional composition according to the present invention is the antibacterial and whitening functional composition containing Quercus acutissima sap as a main component, which is effective in preventing and treating various skin diseases such as acne, atopic diseases or folliculitis, and also has skin whitening functions.

Description

Antibacterial and whitening-functional composition containing Quercus Acutissima Sap}

The present invention relates to an antibacterial and whitening functional composition containing oak sap.

Specifically, the present invention is an antibacterial and whitening functional composition containing oak sap as a main component, and is effective in the prevention and treatment of various inflammatory, fungal or bacterial skin diseases such as acne, atopy or dermatitis, and also has a whitening function. For.

In modern society, patients with inflammatory or bacterial skin diseases such as acne and atopy are increasing due to personal factors such as irregular eating habits, sleeping habits and stress, and social factors such as various pollutants and harmful substances.

In general, acne refers to a chronic inflammatory disease of the follicular sebaceous gland characterized by the formation of comedones, papules, pustules, cysts, or nodules, which occurs mainly in adolescent men and women. The causes of the onset of acne are still not clearly distinguished. However, it has been recognized as the orthodox through the research reports so far that three important causes are directly involved in the onset of acne if genetic and environmental factors are excluded. The first is the excessive secretion of sebum, and the second is the obstruction of the pores due to hyperkeratosis of the hair follicle. The third is due to the presence of Propionibacterium acnes, which is explained as an important underlying cause of exacerbation of acne lesions.

As the proliferation of acne bacteria in the sebaceous gland increases, triglycerides constituting sebum are converted into free fatty acids by acne bacteria and act as an irritant or comeogenic substance. By doing this, it promotes the generation of acne, and it proceeds further to accompany the inflammatory reaction.

On the other hand, the oak tree (Quercus acutissima) is a deciduous tree of the oak family that is 20 to 25 m in height and 1 m in diameter, and the bark is black and gray, and leaves or bark extract or sap are used as additives such as cosmetics or food. It is widely used in the field.

In particular, studies on the antibacterial, antiviral, or anti-inflammatory effects of oak leaves, bark or sap, etc. are being actively conducted, and studies on the additional efficacy of extracts, fractions, or combinations thereof as external preparations or cosmetics for the skin are continuously conducted. Is losing.

KR Registered Patent Publication No. 10-0548033

The present invention provides a composition containing the sap and bark extract of a natural material, oak tree, and also contains a natural preservative, so that there is no side effect to the human body and has excellent antibacterial and whitening improvement effects.

The present invention inhibits the growth of Staphylococcus epidermidis, which is a secondary infection as well as primary sensitive bacteria of acne, while simultaneously promoting an important step in the formation of melanin, a skin pigmentation pigment. It provides an antibacterial and whitening functional composition that inhibits the activity of known tyrosinase.

The present invention also provides a cosmetic composition comprising the antibacterial and whitening functional composition.

The present invention further provides a method for preparing the antibacterial and whitening functional composition.

The present invention is conceived to solve the above problems, and is an antibacterial and whitening functional composition that inhibits the growth of Staphylococcus epidermidis strains and inhibits the activity of tyrosinase, the antibacterial And it relates to a cosmetic composition comprising a whitening functional composition and a method of manufacturing the same.

The antibacterial and whitening functional composition comprises the steps of generating an oak tree sap water vapor by putting the oak wood chips into a drying furnace where the outside is trapped in a steam tank and drying them, and making the oak tree sap water vapor in contact with the cooling coil in the cooling tank. 40 to 60 parts by weight of an oak sap prepared, including the step of collecting the generated condensate; 20 to 40 parts by weight of the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the bark of oak tree; 0.05 to 2 parts by weight of aqueous zinc gluconate; 0.1 to 3 parts by weight of chopi tree fruit extract; 0.1 to 3 parts by weight of palisade flower extract; And 0.1 to 3 parts by weight of Usnia extract. In addition, the antibacterial and whitening functional composition contains more of the oak sap than the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark, and Staphylococcus epidermidis inhibits the growth of strains , It is characterized by inhibiting the activity of tyrosinase (tyrosinase).

The antibacterial and whitening functional composition, for example, 50 to 60 parts by weight of oak sap; And it may contain 25 to 35 parts by weight of the ethyl acetate fraction of the high pressure and ultrasonic combination extract of oak bark.

In one example, the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark, washing and pulverizing the oak bark and drying; After preparing a sample by mixing the dried oak bark with distilled water, the sample was cooled to a low temperature for 5 to 15 minutes under a temperature condition of 20° C. to 30° C. and a pressure condition of 4,000 bar to 6,000 bar using a high pressure extraction device. High pressure extraction; Ultrasonically extracting the sample after the low-temperature high-pressure extraction for 30 to 90 minutes using an ultrasonic extractor under a frequency condition of 40 kHz to 70 kHz; Extracting the sample after the ultrasonic extraction using distilled water with hot water for 10 to 20 hours under a temperature condition of 40°C to 60°C to obtain a high pressure and ultrasonic combination extract of the bark of an oak tree; Filtration and concentration of the high-pressure and ultrasonic combination extract, lyophilization and powdering; And dispersing the powdered high-pressure and ultrasonic combination extract in distilled water and then solvent fractionation using ethyl acetate to obtain an ethyl acetate fraction of the high-pressure and ultrasonic combination extract of an oak bark.

The aqueous zinc gluconate contains, for example, purified water as a main component, and may contain a zinc salt of gluconic acid represented by Formula 1 below in a range of 50,000 ppm to 100,000 ppm.

[Formula 1]

The antibacterial and whitening functional composition may further include, for example, 0.5 to 5 parts by weight of a viscosity modifier.

In one example, the viscosity modifier may be any one or more selected from butylene glycol, hexylene glycol, ethoxydiglycol, and dipropylene glycol.

In one example, the Chopi tree fruit extract, the Pasqueflower extract, and the Usnia extract are respectively subjected to ultrasonic pre-treatment for 30 minutes to 60 minutes under conditions of 100 kHz to 200 kHz using an ultrasonic extractor for barberry fruit, pasqueflower, and ginkgo leaves. After that, it may be an ultrasonic low-temperature water extract prepared by extracting low-temperature water for 6 to 24 hours at a temperature range of 20°C to 40°C.

The present invention also relates to a cosmetic composition comprising the antibacterial and whitening functional composition.

The present invention also relates to a method for preparing the antibacterial and whitening functional composition.

The method for preparing the antibacterial and whitening functional composition is a method for producing a composition, characterized in that it inhibits the growth of Staphylococcus epidermidis strains and inhibits the activity of tyrosinase. An oak tree that includes generating water vapor from an oak tree by putting wood chips into a drying furnace where the outside is trapped in a steam tank and drying it, and collecting the condensed water generated by contacting the water vapor from the oak tree into a cooling coil in a cooling tank. Obtaining tree sap; After washing, high-pressure and ultrasonic extraction are sequentially performed using a high-pressure extraction device and an ultrasonic extractor using a high-pressure extraction device and an ultrasonic extractor to prepare a high-pressure and ultrasonic combination extract, and then freeze the high-pressure and ultrasonic combination extract. Solvent fractionation of the dried product using ethyl acetate to obtain an ethyl acetate fraction of the high pressure and ultrasonic combination extract of the bark of an oak tree; And 40 to 60 parts by weight of the oak sap, 20 to 40 parts by weight of the ethyl acetate fraction of the high-pressure and ultrasonic combination extract of the bark of the oak tree, 0.02 to 2 parts by weight of aqueous zinc gluconate, 0.1 to 3 parts by weight of the Chopi tree fruit extract. It includes; mixing 0.1 to 3 parts by weight of Pasqueflower extract and 0.1 to 3 parts by weight of Usnia extract.

In one example, obtaining an ethyl acetate fraction of a high pressure and ultrasonic combination extract of an oak bark includes washing, pulverizing and drying the oak bark; After preparing a sample by mixing the dried oak bark with distilled water, the sample was cooled to a low temperature for 5 to 15 minutes under a temperature condition of 20° C. to 30° C. and a pressure condition of 4,000 bar to 6,000 bar using a high pressure extraction device. High pressure extraction; Ultrasonically extracting the sample after the low-temperature and high-pressure extraction for 30 to 90 minutes using an ultrasonic extractor under a frequency condition of 40 kHz to 70 kHz; Extracting the sample after the ultrasonic extraction using distilled water with hot water for 10 to 20 hours in a temperature condition of 40°C to 60°C to obtain a high-pressure and ultrasonic combination extract of the bark of an oak tree; Filtration and concentration of the high-pressure and ultrasonic combination extract, lyophilization and powdering; And dispersing the powdered high pressure and ultrasonic combination extract in distilled water and then solvent fractionation using ethyl acetate to obtain an ethyl acetate fraction of the high pressure and ultrasonic combination extract of an oak bark.

The antibacterial and whitening functional composition according to the present invention contains natural antibacterial and whitening functional ingredients obtained from oak, and also contains an eco-friendly preservative, so that there is no side effect on the human body, and it is skin-friendly and environmentally friendly.

The antibacterial and whitening functional composition according to the present invention is also antimicrobial against the acne causative bacteria Propionibacterium acnes , as well as the secondary skin infections Staphylococcus epidermidis after acne infection. Not only does it have an excellent effect in improving acne due to its excellent action, but also has the effect of inhibiting the activity of tyrosinase, which is known to play a role in promoting an important step in the formation of melanin, a skin pigmentation pigment.

Of course, the effect of the present invention is not limited within the above-mentioned range.

Figure 1 shows the results of the cytotoxicity test for the oak sap and oak bark extract and fractions according to the present invention.
Figures 2 and 3 show the results of the antibacterial activity test for the oak sap and oak bark extract and fractions according to the present invention.
Figure 4 shows the results of the tyrosinase inhibitory activity for the oak sap and oak bark extract and fractions according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to drawings and examples.

In this specification, expressions in the singular include plural expressions unless otherwise specified.

The terms used in the present specification have selected general terms that are currently widely used as possible while considering functions in the present invention, but this may vary according to the intention or precedent of a technician working in the field, the emergence of new technologies, and the like. In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning of the terms will be described in detail in the description of the corresponding invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall contents of the present invention, not a simple name of the term.

Since the embodiments of the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the scope of the specific embodiment, it should be understood to include all the conversions, equivalents, and substitutes included in the invented spirit and technical scope. In describing the embodiments, when it is determined that a detailed description of a related known technology may obscure the subject matter, the detailed description thereof will be omitted.

In the present specification, terms such as "comprise" or "consist" are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other It is to be understood that the presence or addition of features, numbers, steps, actions, components, parts, or combinations thereof, does not preclude in advance the possibility of being excluded.

In the present specification, terms such as “consisting of” or “consisting of” are used to emphasize that other configurations other than features, numbers, steps, operations, components, parts, or combinations thereof described in the specification are not included. , In addition to the above configuration, it is to be understood that the presence or addition of one or more other features, numbers, steps, operations, components, parts, or combinations thereof are excluded.

In the present specification, the term "main component" means a component included in the composition in the largest proportion of all components in the composition according to the present invention. For example, “containing the sap of the oak tree as a main component” means that the composition contains the sap of the oak tree in the highest proportion.

The present invention relates to an antibacterial and whitening functional composition, a cosmetic composition comprising the same, and a method of manufacturing the same.

The antibacterial and whitening functional composition according to the present invention is a composition for external application or cosmetic for skin containing an oak component, specifically an oak sap, and an ethyl acetate fraction of a high pressure and ultrasonic extract of an oak bark.

In general, the components obtained or extracted from the oak tree exist in various ways, such as leaf extract, sap or bark extract, etc., but the content in a composition that provides an antibacterial effect, particularly a composition that provides an antibacterial effect as a skin external preparation or cosmetic It is common to be insignificant enough to be just an auxiliary ingredient.

The inventors of the present invention contain an oak component, specifically, an ethyl acetate fraction of a high pressure and ultrasonic extract of an oak bark along with an oak sap as a main component, and when adding a natural preservative component and other skin external preparations or cosmetic components, antibacterial By confirming that a composition having a significantly improved effect can be prepared, the present invention has been completed.

The present inventors are also known to play a role in promoting an important step in the formation of melanin, a skin pigmentation pigment, in addition to the antimicrobial effect, when the composition contains the ethyl acetate fraction of the high pressure and ultrasonic extract of the bark of the oak tree as a main component. It was confirmed that it has an effect of inhibiting the activity of tyrosinase, and the present invention was completed.

In particular, the antibacterial and whitening functional composition according to the present invention includes an oak sap prepared from an oak sap production process that minimizes the incorporation of impurities in the oak sap, and can also maximize the extraction of antibacterial and whitening functional active ingredients. By including the ethyl acetate fraction of the high pressure and ultrasonic extract of the oak bark produced from the extraction and fractionation process of the oak bark, there is no mixing of impurities, and it is harmless to the human body as it contains natural ingredients as the main component. It has excellent antibacterial action against Staphylococcus epidermidis, a skin infectious bacteria, so it has excellent acne improvement effect, and also has a whitening function because it has an effect of inhibiting the activity of tyrosinase.

The antibacterial and whitening functional composition according to the present invention comprises 40 to 60 parts by weight of oak sap; 20 to 40 parts by weight of the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the bark of oak tree; 0.05 to 2 parts by weight of aqueous zinc gluconate; 0.1 to 3 parts by weight of chopi tree fruit extract; 0.1 to 3 parts by weight of palisade flower extract; And 0.1 to 3 parts by weight of Usnia extract; containing, Staphylococcus epidermidis ( Staphylococcus epidermidis ) It is characterized in that it inhibits the growth of strains and inhibits the activity of tyrosinase (tyrosinase).

The oak sap serves not only as a solvent, but also provides antibacterial activity against Staphylococcus epidermidis , a secondary skin infection after acne infection, and contributes to inhibiting the activity of tyrosinase. Configuration.

General oak sap is prepared by cutting the oak wood, extracting the sap by performing ultrasonic treatment, etc., and filtering it using activated carbon or a filter.In this case, impurities are mixed in the oak sap and the purity of the sap There is a problem that is lowered. However, since the oak sap contained in the composition of the present invention is condensed water produced by generating steam of oak sap in a drying furnace where the outside is trapped in a steam tank and condensing it in a cooling tank, there is no mixing of impurities. There is an advantage of high purity, and such high purity oak sap may contribute to improving the antibacterial effect of the composition and providing whitening functionality.

Specifically, the oak sap according to the present invention is the main component that accounts for the largest proportion of the components contained in the composition, and by putting the oak wood chips into a drying furnace that is captured by a steam tank outside and drying them, it generates water vapor of the oak tree sap. And collecting the generated condensate by bringing the water vapor of the oak sap into contact with the cooling coil in the cooling tank.

The oak sap according to the present invention may be prepared using, for example, a tree sap extraction device disclosed in Korean Patent Publication No. 10-2072667, but is not limited thereto, and according to the drying of the oak tree Any method of obtaining an oak sap through condensation of the generated water vapor may be adopted and used without limitation in the present invention.

The oak sap is contained within the range of 40 to 60 parts by weight as a main component in the composition. In another example, the oak sap may be included in the range of 50 to 60 parts by weight or 55 to 60 parts by weight in the composition. In the present specification, the term "parts by weight" means a weight ratio between each component unless otherwise expressed.

The antibacterial and whitening functional composition according to the present invention contains more oak sap than the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak tree bark, which is a high pressure and ultrasonic combination extract of the oak tree sap in the composition In the case of containing less than the ethyl acetate fraction of, the antimicrobial effect of the composition and the inhibitory effect of tyrosinase activity are not maximized, and the viscosity or physical properties of the composition and miscibility with other substances may not be smoothly achieved, which is undesirable. Because not.

In one embodiment, the antibacterial and whitening functional composition according to the present invention is 50 to 60 parts by weight of oak sap; And it may contain 25 to 35 parts by weight of the ethyl acetate fraction of the high pressure and ultrasonic combination extract of oak bark.

The antimicrobial composition according to the present invention also includes an ethyl acetate fraction of a high pressure and ultrasonic combination extract of an oak bark.

The ethyl acetate fraction of the high pressure and ultrasonic combination extract of oak bark contributes to the improvement of growth inhibition against Staphylococcus epidermidis strain, a secondary skin infection after acne infection, and also tyrosinase ) As a constitution contributing to the inhibition of activity, it was obtained by a method of producing an ethyl acetate fraction of an extract in combination with high pressure and ultrasonic waves, which is an extraction method capable of effectively extracting the physiologically active active ingredient in the bark of oak.

There are various methods of extracting the oak bark, such as organic solvent extraction, hot water extraction, or ultrasonic extraction, and the present inventor performs hot water extraction after simultaneous high pressure and ultrasonic extraction of the oak bark through various experiments, In the case of including the ethyl acetate fraction of the high pressure and ultrasonic extract of the oak bark obtained by fractionating the ethyl acetate solvent together with the oak sap, the growth inhibitory effect and tyrosinase on the Staphylococcus epidermidis strain It was confirmed that the (tyrosinase) activity inhibitory effect was maximized.

The ethyl acetate fraction of the high pressure and ultrasonic combination extract of oak bark is contained within the range of 20 to 40 parts by weight in the composition. In another example, the ethyl acetate fraction of the high pressure and ultrasonic combination extract of oak bark may be included in the range of 25 to 35 parts by weight or 30 to 35 parts by weight in the composition.

The ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark, for example, washing and pulverizing the oak bark and drying; After preparing a sample by mixing the dried oak bark with distilled water, the sample was cooled to a low temperature for 5 to 15 minutes under a temperature condition of 20° C. to 30° C. and a pressure condition of 4,000 bar to 6,000 bar using a high pressure extraction device. High pressure extraction; Ultrasonically extracting the sample after the low-temperature and high-pressure extraction for 30 to 90 minutes using an ultrasonic extractor under a frequency condition of 40 kHz to 70 kHz; Extracting the sample after the ultrasonic extraction using distilled water with hot water for 10 to 20 hours in a temperature condition of 40°C to 60°C to obtain a high-pressure and ultrasonic combination extract of the bark of an oak tree; Filtration and concentration of the high-pressure and ultrasonic combination extract, lyophilization and powdering; And dispersing the powdered high-pressure and ultrasonic combination extract in distilled water and then solvent fractionation using ethyl acetate to obtain an ethyl acetate fraction of the high-pressure and ultrasonic combination extract of the bark of an oak bark. .

As described above, when the bark of an oak tree is sequentially extracted at low temperature and high pressure and ultrasonically, the antibacterial active ingredient in the bark of an oak tree can be effectively extracted, and when the solvent is sequentially fractionated using ethyl acetate, Staphylococcus epidermidis ( Staphylococcus epidermidis ) It is possible to stably obtain an ethylacetate fraction of an extract of a high pressure and ultrasonic combination of oak bark, which has excellent inhibitory effect on growth and inhibition of tyrosinase activity against the strain.

The antibacterial and whitening functional composition according to the present invention comprises aqueous zinc gluconate.

The aqueous zinc gluconate is a component that enhances the antibacterial effect and plays the role of a preservative and skin conditioning agent, and is included in the composition within a range of 0.05 to 2 parts by weight.

In another example, the aqueous zinc gluconate may be included in the composition in an amount of 0.1 to 1.5 parts by weight or 0.15 to 1.5 parts by weight.

On the other hand, general zinc gluconate has white granules or crystalline powder, but the aqueous zinc gluconate contained in the composition according to the present invention is a water-soluble zinc gluconate soluble in purified water by applying a special method. The zinc salt of gluconic acid contained as a main component and represented by the following Chemical Formula 1 may be included in the range of 50,000 ppm to 100,000 ppm.

[Formula 1]

In the above, "contains purified water as a main component" means that it contains the highest proportion of purified water among the constituents of aqueous zinc gluconate. Specifically, the aqueous zinc gluconate may contain 50% by weight or more, 60% by weight or more, 70% by weight or more, 80% by weight or more, or 90% by weight or more of purified water, and the upper limit of the purified water content is, for example It may be 98% by weight or less, 97% by weight or less, or 95% by weight or less.

The antibacterial and whitening functional composition according to the present invention may further include a component that enhances the antibacterial effect in addition to the aqueous zinc gluconate.

In one example, the antibacterial and whitening functional composition according to the present invention may further include 0.05 to 1 part by weight of aqueous copper gluconate and 0.05 to 1 part by weight of aqueous magnesium gluconate. The aqueous copper gluconate and aqueous magnesium gluconate may be a water-soluble component containing a copper salt of gluconic acid and a magnesium salt of gluconic acid in a range of 50,000 ppm to 100,000 ppm, respectively.

The antimicrobial and whitening functional composition according to the present invention comprises a chopidae fruit extract, a pasqueflower extract, and a Usnia extract as natural preservatives, respectively, in the range of 0.1 to 3 parts by weight.

The antibacterial and whitening functional composition according to the present invention may further improve the effect of preventing and treating various skin diseases such as acne by using an eco-friendly ingredient that has good skin affinity as a preservative and is environmentally friendly and harmless to the human body.

On the other hand, when considering the function and role as natural preservatives, the Chopi tree fruit extract, pasqueflower extract, and Usnia extract contained in the antibacterial and whitening functional composition according to the present invention minimize cell destruction or transformation of active ingredients, and active ingredients It is preferable to use an extraction method capable of effectively performing extraction of, specifically, extracted from low-temperature water and an ultrasonic parallel extraction method.

In one example, the Chopi tree fruit extract, Pasqueflower extract, and Usnia extract were respectively subjected to ultrasonic pre-treatment for 30 minutes to 60 minutes under conditions of 100 kHz to 200 kHz using an ultrasonic extractor for barberry fruit, pasqueflower, and ginkgo leaves. After performing, it may be an ultrasonic low-temperature water extract prepared by extracting low-temperature water for 6 to 24 hours at a temperature range of 20°C to 40°C.

The antibacterial and whitening functional composition according to the present invention may further include a viscosity modifier.

Specifically, the antibacterial and whitening functional composition may further include 0.5 to 5 parts by weight of a viscosity modifier.

The viscosity modifier may be, for example, any one or more selected from butylene glycol, hexylene glycol, ethoxydiglycol, and dipropylene glycol, but is not limited thereto.

The antibacterial and whitening functional composition according to the present invention may further contain purified water in addition to the above-described components. Purified water may be included in the composition in the range of, for example, 0.1 to 5 parts by weight or 0.1 to 3 parts by weight.

The antimicrobial and whitening functional composition according to the present invention comprises the above-described components, it is characterized in that it inhibits the growth of Staphylococcus epidermidis strains and inhibits the activity of tyrosinase. In addition, the antibacterial composition according to the present invention can inhibit the growth of Propionibacterium acnes, which is widely known as the acne causative bacteria.

The present invention also relates to a cosmetic composition comprising the antibacterial and whitening functional composition.

The cosmetic composition may be prepared in, for example, an ointment, solution, cream, emulsion, lotion, lotion, gel, essence, foundation, pack, stick, bath agent, or soap formulation.

The present invention also relates to a method for preparing the antibacterial and whitening functional composition.

The method of preparing the antibacterial and whitening functional composition includes generating oak sap water vapor by putting the oak wood chips into a drying furnace where the outside is trapped in a steam tank and drying them, and the cooling coil and the oak tree sap vapor in a cooling tank. Obtaining an oak sap comprising collecting the condensed water generated by contacting it: After washing, a sample containing dried oak bark is sequentially subjected to high pressure and ultrasonic extraction using a high pressure extraction device and an ultrasonic extractor, and hot water extraction After preparing the high-pressure and ultrasonic combination extract, the freeze-dried product of the high-pressure and ultrasonic combination extract is subjected to solvent fractionation using ethyl acetate, thereby obtaining an ethyl acetate fraction of the high-pressure and ultrasonic combination extract of the bark of an oak tree; And 40 to 60 parts by weight of the oak sap, 20 to 40 parts by weight of the ethyl acetate fraction of the high-pressure and ultrasonic combination extract of the bark of the oak tree, 0.02 to 2 parts by weight of aqueous zinc gluconate, 0.1 to 3 parts by weight of the Chopi tree fruit extract. It includes; mixing 0.1 to 3 parts by weight of Pasqueflower extract and 0.1 to 3 parts by weight of Usnia extract.

In addition, the step of obtaining the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark may include, for example, washing, pulverizing and drying the oak bark; After preparing a sample by mixing the dried oak bark with distilled water, the sample was cooled to a low temperature for 5 to 15 minutes under a temperature condition of 20° C. to 30° C. and a pressure condition of 4,000 bar to 6,000 bar using a high pressure extraction device. High pressure extraction; Ultrasonically extracting the sample after the low-temperature and high-pressure extraction for 30 to 90 minutes using an ultrasonic extractor under a frequency condition of 40 kHz to 70 kHz; Extracting the sample after the ultrasonic extraction using distilled water with hot water for 10 to 20 hours in a temperature condition of 40°C to 60°C to obtain a high-pressure and ultrasonic combination extract of the bark of an oak tree; Filtration and concentration of the high-pressure and ultrasonic combination extract, lyophilization and powdering; And dispersing the powdered high pressure and ultrasonic combination extract in distilled water and then solvent fractionation using ethyl acetate to obtain an ethyl acetate fraction of the high pressure and ultrasonic combination extract of an oak bark.

According to the manufacturing method according to the present invention, there is no side effect on the human body, skin-friendly and eco-friendly, as well as Propionibacterium acnes , which is a cause of acne, as well as Staphylococcus epidummy, a secondary skin infection after acne infection. It has an excellent antibacterial action against Staphylococcus epidermidis , which is excellent in improving acne, and also has an effect of inhibiting the activity of tyrosinase, which is known to play a role in promoting an important step in the formation of melanin, a skin pigmentation pigment. A composition having a whitening function is prepared.

Hereinafter, the antibacterial and whitening functional composition according to the present invention and the acne improvement and whitening effect experiment of the flexible lotion including the same will be described with reference to Examples and Comparative Examples.

[Preparation Example 1]-Preparation of condensate water vapor from oak sap

Using the tree sap extraction apparatus according to Korean Patent Publication No. 10-2072667, the condensed water of the sap sap of the oak was prepared.

Specifically, after inserting the oak wood chips steamed for 10 minutes at a pressure of 25 kg/cm in the chip drying furnace, the high-heated oak sap water vapor generated by the steam tank surrounding the chip drying furnace is removed with the cooling coil in the cooling tank. By making contact, condensed water (Sap of Sap of Sap of oak) was produced.

[Preparation Example 2]-Preparation of ethyl acetate fraction of an extract of high pressure and ultrasonic combination of bark of oak tree

The bark was separated from the oak tree using a bark remover (peeler, HJ1288), washed with water, and pulverized through a blender. The finely ground bark was dried at 50° C., and then stored in a refrigerator at 4° C. to obtain a combination of high pressure and ultrasonic extraction and a fraction thereof.

Specifically, the dried oak bark was mixed with distilled water in a ratio of 1:10 to prepare a sample, and this was prepared at 25℃ using a high-pressure extraction device (FOOD CIP-70-350-80, Ilshin Autoclave, Daejeon, Korea). After extracting the sample at low temperature and high pressure for 10 minutes under temperature and pressure conditions of 5,000 bar, ultrasonic extraction was performed for 30 minutes at 60 kHz using an ultrasonic extractor (AUG-R3-900, ASIA ULTRASONIC, Gyeonggi, Korea). By performing high pressure and ultrasonic extraction in parallel. Subsequently, hot water extraction was performed for 10 hours at a temperature condition of 50° C. in an extraction flask equipped with a vertical reflux condenser using distilled water equivalent to 10 times that of the sample, thereby obtaining a parallel extract of high pressure and ultrasonic waves of oak bark. Subsequently, the high pressure and ultrasonic combination extract was filtered using a vacuum filtration pump (KNF Laboport Pressure Pump, ColeParmer, Vernon Hills, IL, USA) and 20 to 25 μm filter papers of Whatman (Piscataway, NJ, USA), It was concentrated using a rotary vacuum evaporator NN series, EYELA, Rikakikai Co., Tokyo, Japan, and powdered using a freeze dryer (PVTFA 10AT, ILSIN, Suwon, Korea). Subsequently, the powdered high-pressure and ultrasonic combination extract was dispersed in distilled water, and solvent fractionated using ethyl acetate to obtain an ethyl acetate fraction of the high-pressure and ultrasonic combination extract of the bark of oak bark, and the fractionated sample was filtered in the same manner. After concentration and freeze-drying, it was stored in the refrigerator.

[Production Example 3]-Preparation of natural preservative ingredients

Individual samples are prepared by performing pre-treatment of washing and crushing each of the barberry fruit, pasqueflower, and ginkgo leaves, and this is 40 at 150 kHz using an ultrasonic extractor (AUG-R3-900, ASIA ULTRASONIC, Gyeonggi, Korea). After ultrasonic extraction for a minute, by using distilled water equivalent to 10 times of the sample in an extraction flask equipped with a vertical reflux cooler and extracting low temperature water for 10 hours at a temperature of 25°C. Were prepared respectively.

[Comparative Preparation Example 1]-Preparation of hot water extract of bark of oak tree

The same as the oak bark used in Preparation Example 2 was extracted with hot water at 100°C for 24 hours using a hot water extractor (TL-6Point(K), Misung Scientific Co., Yangjoo, Korea) to obtain a hot water extract of the oak bark. Obtained. Subsequently, the hot water extract of bark of oak was powdered in the same manner as in Preparation Example 2.

[Comparative Preparation Example 2]-Preparation of ethanol extract of bark of oak

Using the same bark used in Preparation Example 2, and using 75% ethanol as an extraction solvent, reflux extraction was performed by repeating three times at 80° C. for 2 hours each. The extract was powdered in the same manner as in Preparation Example 2.

[Comparative Preparation Example 3]-Preparation of chloroform fraction of hot water extract of bark of oak tree

After dispersing the powder of the hot-water extract of oak bark prepared according to Comparative Preparation Example 1 in distilled water and solvent fractionation using chloroform, a chloroform fraction of the hot-water extract of oak bark was obtained, and the fractionated sample was filtered and concentrated in the same manner. Refrigerated after freeze drying.

[Comparative Preparation Example 4]-Preparation of chloroform fraction of ethanol extract of bark of oak tree

After dispersing the powder of the ethanol extract of oak bark prepared according to Comparative Preparation Example 2 in distilled water and solvent fractionation using chloroform, a chloroform fraction of the ethanol extract of oak bark was obtained, and the fractionated sample was filtered and concentrated in the same manner. Refrigerated after freeze drying.

[Experiment preparation]-Experimental strain and culture

In the experimental example in accordance with the present invention acne causing bacteria of Propionibacterium sludge tumefaciens arc Ness (Propionibacterium acnes, KCTC3314), inflammatory or bacterial skin diseases induced yeast (Yeast) Candida albicans (Candida albicans, ATCC 10231), merchandise skin of the Gram-positive Staphylococcus epidermidis (KTTC12228) was used. Propionibacterium acnes (KCTC3314) was inoculated in Reinforced clostridial (RC) medium, sealed using a Gaspack system in an anaerobic culture tank, and then anaerobic cultured at 37°C for 2 days. Aerobic strains of Candida albicans (Candida albicans, ATCC 10231) are Sabaurd Dextrose Agar was used (Difco. USA), and cultured and then inoculated with the bacteria for 24 hours at 25 ℃ incubator. In addition, Staphylococcus epidermidis (KTTC12228), an aerobic strain, was inoculated in Mueller-Hinton medium and cultured at 37° C. for 24 hours.

[Experimental Example 1]-Cytotoxicity test

Human acute monocytic leukemia cells THP-1 (Human acutemonocytic leukemia) cultured in RPMI-1640 medium without fetal bovine serum (FBS) were cultured in a 96-well microplate at a concentration of 5 x 104 cells/well, Preparation Example 1 , 2 and the samples according to Comparative Preparation Examples 1 to 4 were administered by concentration, cultured for 24 hours, and then the supernatant was removed, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) According to the measurement method, 100 ul of the MTT solution was treated at 36° C. for 4 hours. The culture solution was removed, treated with 50 ul of DMSO, and measured with an absorbance meter (ELISA) at 540 nm, and the measured value was calculated in the same manner as in Equation 1 below.

[Equation 1]

Cytotoxicity (%) = (1-absorbance of sample added group/absorbance of no added group) × 100

As a result, when each extract was treated with the cells at a concentration of 100 ug/ml, the survival rate of the cells was 95% or more, so that the sap, bark extract and bark fraction according to Preparation Examples 1 and 2 and Comparative Preparation Examples 1 to 4 It was confirmed that it did not affect the survival of these cells (see Fig. 1).

[Example 2] - propionic sludge tumefaciens arc Ness (Propionibacterium acnes, KCTC3314) and Candida albicans antibiotic activity tests on (Candida albicans, ATCC 10231)

The propionic sludge tumefaciens arc Ness (Propionibacterium acnes, KCTC3314) and Candida Albi antimicrobial activity for kanseu (Candida albicans, ATCC 10231) of fraction samples according to Preparation Example 2 was measured in the following manner.

1) Preparation of bacteria solution

The initial culture solution of the strain to be tested is serially diluted 10 times with PBS (Phosphate Buffer Saline) buffer solution, and prepared to be 1 Х10 ⁶ per ml of bacterial solution.

2) Preparation of test group and control group

The test group was inoculated (1%) with 10 µl of the bacterial solution to 1 ml of the fraction according to Preparation Example 2, and then well suspended and left at room temperature for 1 to 15 minutes. The control group was inoculated (1%) with 10 µl of bacterial solution in 1 ml of PBS (Phosphate Buffer Saline) buffer solution, suspended well, and left at room temperature.

3) Inoculation of the medium of the test group

A liquid medium (Propionibacterium acnes) for each strain in each of the time periods (1 minute, 3 minutes, 5 minutes, 10 minutes, 15 minutes) in the left test group (composition of Preparation Example 2 inoculated with bacteria solution) , KCTC3314); RCB, Candida albicans ( Candida albicans , ATCC 10231); SDB) 9ml and vortexing (Vortexing).

Subsequently, the test group and the liquid medium mixture were divided into two or more plate mediums by 0.1 ml each and dispensed into the petri dish, and left to stand in a 48° C. water bath for 30 minutes or more, and then 15 to 15 to SDA, which was a medium for fungi. Dispense 20ml and mix evenly. Afterwards, bacteria (Propionibacterium acnes, KCTC3314) are 37.5°C±2.5°C / (2~3 days) fungi ( Candida albicans , ATCC 10231)) are 25°C±2.5°C / ( 3~5 days).

4) Inoculation of control medium

As a control group, 9 ml of PBS (Phosphate Buffer Saline) buffer was added and vortexed.

Then, divide the control group into two or more plate mediums by dividing 0.1 ml each into the Petri dish, and then dispensing 15-20 ml of RCA, a culture medium for bacteria and SDA, a culture medium for fungi, left for 30 minutes or more in a water bath at 48°C. Mix it. After that, incubate bacteria at 37.5℃±2.5℃ / (2~3 days) and fungi at 25℃±2.5℃ / (3~5 days).

5) Calculation and evaluation of inhibition rate (%)

The number of colonies formed in each group is calculated, and the inhibition rate (%) of the test group compared to the control group is calculated by Equation 2 below.

[Equation 2]

Inhibition rate (%) = [Number of control group (CFU)-number of test group (CFU)] / number of control group (CFU) X 100

6) result

Sludge propynyl fraction according to Preparation Example 2 tumefaciens arc Ness (Propionibacterium acnes, KCTC3314) and Candida Albi for kanseu antibacterial activity test results for (Candida albicans, ATCC 10231), the anaerobic bacteria Propionibacterium acnes (KCTC 3314) bacteria inoculation It shows a sterilization effect of 99.9% or more after 1 minute, and for the yeast Candida albicans (ATCC 8739), it is measured to exhibit a sterilization effect of 99.9% or more 5 minutes after inoculation of the bacteria, and the fraction according to Preparation Example 2 is Propionibacterium arc Ness ( Propionibacterium acnes , KCTC3314) and Candida albicans ( Candida albicans , ATCC 10231) was confirmed to have excellent antibacterial properties ([Table 1], see FIGS. 2 and 3).

Propionic sludge antimicrobial activity results for tumefaciens arc Ness (Propionibacterium acnes, KCTC3314) and Candida albicans (Candida albicans, ATCC 10231) Strain to be tested Inoculated
Count
(cfu/ml) object Contact Time / Result(%) 1M 3M 5M 10M 15M Propionibacterium acnes (KCTC 3314) 8.7x 10 ³ Test group (Preparation Example 2 + strain) 99.9 99.9 99.9 99.9 99.9 Candida Albicans
(ATCC 10231) 1.26x 10 ⁴ Test group (Preparation Example 2 + strain) 99.6 99.7 99.9 99.9 99.9

[Experimental Example 3]-Antimicrobial activity test against Staphylococcus epidermidis (KTTC12228)

Staphylococcus epidermidis (KTTC12228) antibacterial activity of the components according to Preparation Example and Comparative Preparation Example was measured using a paper disk diffusion method.

Specifically, the evaluation of antibacterial activity against Staphylococcus epidermidis (KTTC12228), a secondary causative agent of acne, was incubated at 37° C. for 24 hours using Muller-Hinton medium, and then at a constant concentration (optical density 620). The bacterial suspension adjusted to absorbance 0.5) at nm was uniformly spread on an RC agar plate using a sterilized cotton swab. Thereafter, the plate was dried and a paper disk (Φ 8mm, Adventec. USA) was placed on the surface of the plate inoculated with the strain, and the samples according to Preparation Example 2 and Comparative Preparation Examples 1 to 4, and the positive control tetracycline and Preparation Example 1 and The mixed sample of 2 (6:4) was absorbed by concentration. After incubating the plate treated with the sample for 3 days using the Gaspack system in an anaerobic culture tank, the antibacterial activity was measured by the size of the inhibition zone around the disk, and the results are shown in [Table 2] below. .

Staphylococcus epidermidis (KTTC12228) antibacterial activity results Zone of inhibition (diameter in mm)* density
sample 5mg 1mg Manufacturing Example 2 15.94±0.21 13.03±0.16 Comparative Preparation Example 1 11.01±0.06 9.13±0.24 Comparative Preparation Example 2 10.01±0.16 8.23±0.19 Comparative Preparation Example 3 11.78±0.24 9.21±0.14 Comparative Preparation Example 4 10.05±0.15 8.36±0.29 Tetracycline 14.92±0.23 12.82±0.43 Preparation Example 1+2 (6:4) 16.41±0.17 14.53±0.22

* Values are mean±SD(n=3).

As shown in [Table 2], when 5 mg and 1 mg of tetracycline, which is a positive control, were treated, the diameter of the inhibitory ring was about 14 mm and 12 mm, respectively, as a result of the high pressure and ultrasonic combination extract of the oak bark according to Preparation Example 2 It was confirmed that the ethyl acetate fraction of Staphylococcus epidermidis (KTTC12228) has excellent antibacterial activity, and hot water extract (Comparative Preparation Example 1) and ethanol extract (Comparative Preparation Example 2) or chloroform thereof It was confirmed that the antibacterial activity was excellent compared to the fraction. In particular, it was confirmed that the mixed sample obtained by mixing the sap of the oak tree according to Preparation Example 1 and the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark according to Preparation Example 2 at an appropriate ratio showed the best antibacterial activity. Therefore, when mixing the ethyl acetate fraction of the extract of oak tree sap and the high pressure and ultrasonic combination of oak tree bark at an appropriate ratio, it is safer than tetracycline, which has limitations in use, such as the appearance of side effects and resistant bacteria, and has no human side effects. It was verified through an experiment that a composition having an excellent antibacterial effect against tea causative bacteria can be prepared.

[Experimental Example 3]-Tyrosinase inhibitory activity test

It is a method of measuring DOPA chrome generated as a result of the action of tyrosinase by a colorimetric method. The samples according to Preparation Example 2 and Comparative Preparation Examples 1 to 4, ascorbic acid as a positive control, and a mixed sample of Preparation Examples 1 and 2 6:4) tyrosinase inhibitory activity was measured.

Specifically, 100 μl of 0.1 M phosphate buffer and 20 μl of sample solution for each concentration were mixed and reacted at room temperature for 5 minutes. In the reaction solution, 30 μl of tyrosinase (1 K unit/ml) dissolved in 0.1 M phosphate buffer and 30 μl of 1.5 mM tyrosine were mixed and reacted at 37°C for 10 minutes. When the reaction was completed, the absorbance was measured at 490 nm, and the inhibitory activity was calculated based on the absorbance of the blank using a buffer solution instead of the sample solution, and the results are shown in [Table 3] and FIG. 4 below.

As shown in Table 3 and FIG. 4, tyrosinase inhibitory activity of a sample prepared by mixing an oak sap according to Preparation Example 1 and a fraction according to Preparation Example 2 at an appropriate ratio (6:4) This was higher than that of other samples, and it was confirmed that the level was higher than that of ascorbic acid, a positive control group. The extract according to Comparative Preparation Examples 1 and 2 and the chloroform fraction according to Comparative Preparation Examples 3 and 4 were also confirmed to have a certain part tyrosinase inhibitory activity, but were measured to be less than 25% based on 5 mg/ml, and Preparation Example 2 It was confirmed that the samples according to and the mixed samples of Preparation Examples 1 and 2 exhibited significantly lower activity compared to those of 85% or more.

Sample concentration
(mg/ml) Tyrosinase inhibitory activity (%)* Manufacturing Example 2 Comparative Preparation Example 1 Comparative Preparation Example 2 Comparative Preparation Example 3 Comparative Preparation Example 4 Ascorbic acid Preparation Example 1+2 (6:4) 0.5 17 8 9 9 11 16 18 2.0 42 12 14 15 16 37 45 3.0 65 16 18 18 19 60 71 5.0 86 21 23 22 24 81 98

* Values are mean±SD(n=3).

[Examples 1 to 4]-Preparation of a flexible lotion formulation composition (A1 to A4)

The aqueous zinc gluconate containing about 60,000 ppm of zinc gluconate together with the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak sap according to Preparation Example 1 and the bark of the oak tree according to Preparation Example 2, according to Preparation Example 4 A composition (A1 to A4) of a flexible lotion formulation was prepared by adjusting the natural preservative component and the viscosity modifier to the contents shown in [Table 4], respectively.

ingredient Content (% by weight) Example 1 (A1) Example 2 (A2) Example 3 (A3) Example 4 (A4) Oak tree sap according to Preparation Example 1 59 55 58 56 Fractions according to Preparation Example 2 31 33 32 35 Aqueous zinc gluconate 0.1 0.15 0.2 0.5 Chorus fruit extract 1.2 1.1 1.5 1.5 Pasqueflower extract 1.1 1.5 0.5 0.7 Usnia extract 1.5 1.4 2 0.8 Viscosity modifier 4 4 5 5 Purified water Balance Balance Balance Balance Sum 100 100 100 100

[Comparative Examples 1 to 3]-Preparation of a flexible lotion formulation composition (B1 to B3)

To prepare a flexible lotion composition (B1 to B3) with the component content as shown in [Table 5] below.

ingredient Content (% by weight) Comparative Example 1 (B1) Comparative Example 2 (B2) Comparative Example 3 (B3) Comparative Example 3 (B3) Oak tree sap according to Preparation Example 1 12 20 - - Fractions according to Preparation Example 2 - - 10 - Aqueous zinc gluconate 0.1 0.15 0.3 0.8 Chorus fruit extract 1.5 1.1 1.5 1.4 Pasqueflower extract 1.4 1.5 0.5 0.6 Usnia extract 1.0 1.4 2 0.9 Viscosity modifier 2 3 1.5 2 Purified water Balance Balance Balance Balance Sum 100 100 100 100

[Experimental Example 4]-Acne improvement effect experiment

In order to find out the effect of improving acne of the softening water composition prepared according to the above Examples and Comparative Examples, a clinical experiment was conducted by the following method.

As subjects, 50 men and women in their late teens to early 30s with acne on their faces were selected. The composition according to the Example was applied to the left side of the face and the composition according to the Comparative Example was applied twice a day in the morning and in the evening on the right side of the face to evaluate the effect of improving acne for 8 weeks. As for the judgment method, according to the condition of the test subject's skin and acne before the start of the experiment, a certain grade was taken and pictures were taken. After 8 weeks, the degree of acne prevention and improvement was judged by photographing and visual observation. It is shown in Table 6].

rank Skin condition criteria 0 Normal state One Very small number of comedones or no inflammation 2 The number or size of the comedones is moderate and inflammation has not progressed 3 A condition in which a small number of inflammatory papules are present 4 The presence of small, extensive crystals and inflammatory comedones 5 The presence of small and small papular nodules 6 Moderate inflammatory nodules present 7 A condition in which a large number of inflammatory nodules are present 8 The presence of severe nodular comedones 9 The presence of severe cysts and inflammatory nodules

According to the criteria of [Table 6] above, if the test subject's acne rating exceeds 2, it is judged as'excellent effect', and if it shows a decrease of 1 to 2, it is judged as'intermediate effect' and decreased. If does not appear, it was judged as'no effect', and if the grade after the test was higher than the grade before the test, it was judged as'deteriorated', and the results are shown in Table 7 below.

Acne improvement effect (n=50, %) Excellent effect Middle effect no effect Worsened Side Effect Example 1 88% 10% 2% - - Example 2 85% 12% 3% - - Example 3 91% 8% One% - - Example 4 89% 10% One% - - Comparative Example 1 - One% 99% - - Comparative Example 2 - 4% 96% - - Comparative Example 3 - 3% 97% - - Comparative Example 4 - 4% 94% 2% -

As can be seen in [Table 7], less than 5% of the subjects were found to be'no effect', and it was confirmed that the softening water according to Examples 1 to 4 of the present invention had very excellent acne improvement effect. On the other hand, in the case of the composition according to the comparative example containing less than the effective content of oak sap, the high pressure of the bark of the oak bark and the ethyl acetate fraction of the ultrasonic extract in less than the effective content, or not containing both components, It showed no effect on improving acne.

[Experimental Example 5]-Whitening improvement effect experiment

A clinical trial was conducted to confirm the skin whitening effect of the cosmetic softening water composition according to the above Examples and Comparative Examples. For 30 women in their 20s to 40s, Examples 1 to 4 were divided into 4 groups of 10 people each, and were used twice a day after washing in the morning and after washing in the evening, and for 4 weeks. The melanin index of the skin before and after use was measured using Mexameter® MX 18 (CK. Electronic GmbH, Cologne, Germany), and the value was ΔM.I. The values were calculated according to the following Equation 3 to compare the effects, and the results are shown in [Table 8].

[Equation 3]

ΔM.I. = M.I. after 4 weeks Value-M.I. value

Softening water ΔM.I. Example 1 -14.05 Example 2 -14.72 Example 3 -15.14 Example 4 -14.33 Comparative Example 1 -4.12 Comparative Example 2 -6.45 Comparative Example 3 -5.62 Comparative Example 4 -3.52

As shown in [Table 8], when the softening water according to Examples 1 to 4 was used for 4 weeks, it was confirmed that the skin whitening effect was higher than that of the comparative example.

Claims (10)

The steps of generating water vapor of an oak tree by putting the oak wood chips into a drying furnace where the outside is captured by a steam tank and drying them, and collecting the generated condensate by bringing the water vapor of an oak tree into contact with the cooling coil in the cooling tank. 50 to 60 parts by weight of oak sap prepared, including;
25 to 35 parts by weight of an ethyl acetate fraction of the high pressure and ultrasonic combination extract of the bark of an oak tree;
0.05 to 2 parts by weight of aqueous zinc gluconate containing purified water as a main component as a water-soluble zinc gluconate soluble in purified water and containing a zinc gluconate salt represented by the following formula (1) in a range of 50,000 ppm to 100,000 ppm;
0.1 to 3 parts by weight of chopi tree fruit extract;
0.1 to 3 parts by weight of palisade flower extract; And
Including; 0.1 to 3 parts by weight of Usnia extract,
The ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark,
Washing and pulverizing the oak bark and drying; After preparing a sample by mixing the dried oak bark with distilled water, the sample was cooled to a low temperature for 5 to 15 minutes under a temperature condition of 20° C. to 30° C. and a pressure condition of 4,000 bar to 6,000 bar using a high pressure extraction device. High pressure extraction; Ultrasonically extracting the sample after the low-temperature and high-pressure extraction for 30 to 90 minutes using an ultrasonic extractor under a frequency condition of 40 kHz to 70 kHz; Extracting the sample after the ultrasonic extraction using distilled water with hot water for 10 to 20 hours in a temperature condition of 40°C to 60°C to obtain a high-pressure and ultrasonic combination extract of the bark of an oak tree; Filtration and concentration of the high-pressure and ultrasonic combination extract, lyophilization and powdering; And dispersing the powdered high-pressure and ultrasonic combination extract in distilled water and then solvent fractionation using ethyl acetate to obtain an ethyl acetate fraction of the high-pressure and ultrasonic combination extract of an oak bark; and
Staphylococcus epidermidis ( Staphylococcus epidermidis ) Antibacterial and whitening functional composition, characterized in that it inhibits the growth of strains and inhibits the activity of tyrosinase (tyrosinase).
[Formula 1]

The method of claim 1,
Antibacterial and whitening functional composition further comprising 0.5 to 5 parts by weight of a viscosity modifier. The method of claim 2,
Viscosity modifier,
Antibacterial and whitening functional composition of any one or more selected from butylene glycol, hexylene glycol, ethoxydiglycol and dipropylene glycol. The method of claim 1,
Chorus fruit extract, pasqueflower extract, and Usnia extract, respectively,
After performing ultrasonic pre-treatment for 30 to 60 minutes at 100 kHz to 200 kHz using an ultrasonic extractor for the fruit of the Chopi tree fruit, pasqueflower, and ginkgo biloba, low temperature for 6 to 24 hours at a temperature range of 20°C to 40°C An antibacterial and whitening functional composition that is an ultrasonic low-temperature water extract prepared by water extraction. A cosmetic composition comprising the antibacterial and whitening functional composition of claim 1. As a method for producing an antibacterial and whitening functional composition according to claim 1,
Including generating oak sap water vapor by putting the oak wood chips into a drying furnace where the outside is trapped in a steam tank and drying them, and collecting the generated condensate by bringing the oak sap water vapor into contact with the cooling coil in the cooling tank. Steps to obtain an oak sap:
After washing, high-pressure and ultrasonic extraction are sequentially performed using a high-pressure extraction device and an ultrasonic extractor using a high-pressure extraction device and an ultrasonic extractor to prepare a high-pressure and ultrasonic combination extract, and then freeze the high-pressure and ultrasonic combination extract. Solvent fractionation of the dried product using ethyl acetate to obtain an ethyl acetate fraction of the high pressure and ultrasonic combination extract of the bark of an oak tree; And
50 to 60 parts by weight of the oak sap, 25 to 35 parts by weight of the ethyl acetate fraction of the extract of the high pressure and ultrasonic combination of the bark of the oak tree, containing purified water as a main component as a water-soluble zinc gluconate soluble in purified water, 0.02 to 2 parts by weight of aqueous zinc gluconate containing the expressed zinc gluconate salt in the range of 50,000 ppm to 100,000 ppm, 0.1 to 3 parts by weight of barberry fruit extract, 0.1 to 3 parts by weight of palisade extract, and 0.1 to Usnia extract Including; mixing 3 parts by weight
The step of obtaining the ethyl acetate fraction of the high pressure and ultrasonic combination extract of the oak bark,
Washing and pulverizing the oak bark and drying; After preparing a sample by mixing the dried oak bark with distilled water, the sample was cooled to a low temperature for 5 to 15 minutes under a temperature condition of 20° C. to 30° C. and a pressure condition of 4,000 bar to 6,000 bar using a high pressure extraction device. High pressure extraction;
Ultrasonically extracting the sample after the low-temperature and high-pressure extraction for 30 to 90 minutes using an ultrasonic extractor under a frequency condition of 40 kHz to 70 kHz;
Extracting the sample after the ultrasonic extraction using distilled water with hot water for 10 to 20 hours in a temperature condition of 40°C to 60°C to obtain a high-pressure and ultrasonic combination extract of the bark of an oak tree;
Filtration and concentration of the high-pressure and ultrasonic combination extract, lyophilization and powdering; And
Dispersing the powdered high-pressure and ultrasonic combination extract in distilled water and then solvent fractionation using ethyl acetate to obtain an ethyl acetate fraction of the high-pressure and ultrasonic combination extract of oak bark;
Staphylococcus epidermidis ( Staphylococcus epidermidis ) inhibits the growth of the strain, and inhibits the activity of tyrosinase (tyrosinase), characterized in that the antibacterial and whitening functional composition manufacturing method.
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