JP2010202604A - Agent for normalization of skin indigenous microorganism, method for normalization of skin indigenous microorganism and dermatological external preparation or cosmetic using the same, and process for their productions - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To development and provide an agent acting above all selectively on proliferated Propionibacterium acnes among skin indigenous microorganisms, without acting on aerobic microorganisms, to make the balance of skin indigenous microorganisms healthy, that is to develop and provide the agent for normalization of skin indigenous microorganisms. <P>SOLUTION: The agent for normalization of skin indigenous microorganisms includes an extract of Coptis japonica as an effective ingredient and further use the same as a dermatological external agent or a cosmetic, to normalize the skin indigenous microorganisms. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

The present invention relates to a normal skin bacterium normalizing agent comprising an oren extract as an active ingredient, and a normal skin bacterium normalizing method, and further includes a skin external preparation or cosmetic containing the normal skin bacterium normalizing agent and the cosmetic. The present invention relates to a method for producing a skin external preparation or cosmetic.

On the surface of healthy human skin, an aggregated skin resident flora is formed by microorganisms called skin resident bacteria. There are individual differences, site differences, seasonal variations, etc., but in the skin resident flora, Acne (Propionibacterium acnes: P. acnes) is the most anaerobic bacterium, and Staphylococcus aureus (Staphylococcus aureus)
aureus: St. aureus) or Staphylococcus epidermidis: St. aureus
epidermidis) inhabit as skin resident bacteria. It has been reported that the skin resident flora plays two contradictory roles of biological defense from other pathogenic microorganisms and disease expression (endogenous infection) (Non-Patent Documents 1 to 5). Examples of biological defense include a barrier function that prevents entry of pathogenic bacteria from the outside. However, if the balance of the normal skin flora is lost due to poor general condition due to trauma, illness, mental stress, cold, dryness, improper washing, etc.
Abnormal skin bacteria such as aureus and P. acnes proliferate, induce various skin symptoms, and cause disease development.

Acne is a chronic inflammatory disease of the hair follicle line system, and its pathological state is multifactorial, but most of the bacteria detected from the acne lesion are known to be P. acnes. Therefore, it is known as one of the factors that it develops due to exacerbation of acne bacteria (Non-patent Document 6). For this reason, conventionally, it has been common to use an external preparation for skin or a cosmetic containing an antibacterial agent and a bactericidal agent for the prevention and treatment of acne.

Conventionally, as a method for suppressing the growth of acne bacteria, a bactericidal agent such as ibuprofen piconol, chlorhexidine gluconate, triclosan, triclocarban, or pionine has been applied to the affected area as a disinfectant. In recent years, plant extracts and the like have also been studied, and acne prevention cosmetics (Patent Document 1) blended with an extract of the genus Salmonella (Patent Document 1), an acne treatment agent containing whey and acorn extract (Patent Document 2). , An anti-acne composition containing a protein extracted from the genus Propionibacterium (patent document 3), a skin external preparation containing proanthocyanidins (patent document 4), an anti-acne composition containing cis-6-hexadecenoic acid and isopropylmethylphenol A fungus composition (Patent Document 5), an acne improving agent containing a Hyssop extract (Patent Document 6) and the like are disclosed.
On the other hand, Auren is a perennial herbaceous plant of the family Ranunculaceae, and its roots and stems are used as medicines, but it is a material that has been used in traditional Chinese medicine and is a safe plant that does not irritate the skin and mucous membranes. . In addition to containing alkaloids such as berberine, coptisine, aurenin, palmatin, and columbamine, auren contains overcone and overclactone. It has long been used in China as a raw material for burn treatment, purulent infection treatment, infectious dermatitis treatment, empyema treatment, palatal inflammation treatment, polymorphic exudative erythema treatment, gastrointestinal medicine, antidiarrheal intestinal medicine Is.

Noble WC, J Med Microbiol, 17 (1984) 1-12 Noble WC, Microbiology of human skin, Lloyd Luke, London (1981) pp. 168-175 Yasuo Asada, Clinical Bacteriology (Lecture), Kodansha (1977) 71-84 Masayuki Kimura et al., JSHI Journal, 96 (2) (1986) 103-107 Tomoko Fukubayashi, FRAGRANCE JOURNAL, (March 2003) 23-28 Journal of Japan Cosmetic Science Association 2003. Vol. 27, no. 1 JP-A-6-172152 JP 2001-97842 A JP 2002-316944 A JP 2004-115466 A JP 2004-189656 A JP 2004-262862 A

All of the anti-acne bacteria compositions disclosed in the above-mentioned documents, such as whey and psyllium extract, are the results of in vitro studies, and in practical use on the skin as in vivo levels, The antibacterial effect was not superior. In addition, when bactericides such as ibuprofen piconol, chlorhexidine gluconate, triclosan, triclocarban, and pionine are used, not only acne bacteria that are skin resident bacteria, but also useful aerobic bacteria, Since the balance of the skin resident bacteria is greatly different from the balance in the normal state and the barrier function of the skin is lowered, it sometimes causes problems such as skin roughness.

Therefore, it works only on acne bacteria grown among skin resident bacteria, does not act on aerobic bacteria, makes the balance of skin resident bacteria healthy, that is, normalizes skin resident bacteria Technology development was necessary.

As a result of intensive studies to solve the above-mentioned problems, the present inventor has a selective antibacterial action that is superior only for acne bacteria, and the topical skin preparation to which the aurene extract is added. Or it discovered that cosmetics were excellent in normalization of skin resident bacteria, and came to complete this invention.

That is, the present invention includes (1) a normal bacterium normalizing agent comprising an auricular extract as an active ingredient, and (2) normalization of the resident skin resident bacteria suppresses the growth of acne bacteria and the growth of aerobic bacteria. The normal skin bacterium normalizing agent according to the above (1), or (3) acne bacterium is P.
(1) or (2) characterized in that the skin resident bacteria normalizing agent according to (1) or (2), and (4) the aerobic bacteria are St. aureus or St. epidermidis It is related with the skin resident bacteria normalizing agent in any one of 1)-(3).

  The present invention also relates to (5) a skin external preparation or cosmetic comprising the normal skin bacterium normalizing agent according to any one of (1) to (4).

Furthermore, the present invention provides (1) a method for normalizing skin resident bacteria characterized by suppressing the growth of acne bacteria in the skin and maintaining the growth of aerobic bacteria with the auren extract, and (2) acne bacteria Or (3) the aerobic bacterium is St. aureus or St. aureus, characterized in that P. acnes is used.
Any one of the above (1) to (3), characterized in that the method for normalizing the skin resident bacteria described in (1) or (2) above, or (4) an aurene extract is used, which is epidermidis It relates to the normalization method of skin resident bacteria of description.

In addition, the present invention provides (1) a method for producing a skin external preparation or cosmetic for normalizing skin resident bacteria, characterized by comprising an oren extract as an active ingredient and adding to the skin external preparation or cosmetic. ) Normalization of skin resident bacteria suppresses the growth of acne bacteria and maintains the growth of aerobic bacteria, the skin external preparation or cosmetic for normalization of skin resident bacteria described in (1) above (3) The method for producing a skin external preparation or cosmetic for normalizing skin resident bacteria according to (1) or (2) above, wherein the acne is P. acnes, 4) The aerobic bacterium is St.
aureus or St. epidermidis, the method for producing a skin external preparation or cosmetic for normalizing skin resident bacteria according to any one of the above (1) to (3); The present invention relates to a method for producing an external preparation for skin or a cosmetic preparation for normalizing resident skin bacteria according to any one of the above (1) to (4).

ADVANTAGE OF THE INVENTION According to this invention, it has the outstanding antibacterial action only with respect to an acne microbe (P.acnes), and it can normalize a skin normal state, ie, a skin normal microbe, the balance of a skin normal microbe.

An embodiment of the present invention will be described.
The auren used for the extract of the present invention is preferably obtained from rhizomes, but the whole oren plant or any part other than roots, for example, leaves, rhizomes, stems, root barks, etc. may be used.

The aurene extract in the present invention is obtained by solvent extraction of the aurene described above. As the solvent used for the extraction, known solvents may be used, for example, water, methanol, ethanol, propanol, isopropanol, butanol, ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,2-pentanediol, diethylene glycol. , Dipropylene glycol, isoprene glycol, hexylene glycol, glycerin, methyl acetate, ethyl acetate, isopropyl acetate, ethyl ether, isopropyl ether, acetone, and other organic solvents can be used, and water and ethanol are preferred. From these extraction solvents, one kind can be used alone, or two or more kinds can be mixed and used.

As a method for producing the auren extract used in the present invention, a conventionally known method can be used. For example, a method for extracting abbrevis or a dried product of auren with water or hot water, or extracting with an organic solvent and filtering, etc. Can be given. The extraction conditions are not particularly limited as long as they are generally used for plant extraction. Further, after extraction and filtration, the extract may be concentrated as necessary. As a concentration method, a conventional method such as an evaporator can be used.

The auren extract in the present invention can be obtained by extraction with the above solvent, but the dry product of auren is 0.001 to 10% by mass (hereinafter, “% by mass” is simply “ % ”), And more preferably 0.01 to 5%. By using in this range, it becomes possible to function as a normal skin bacterium normalizing agent of the present invention.

The extract thus obtained can be used as it is as a skin external preparation or cosmetic of the present invention, and it is also possible to add purification operations such as deodorization and decolorization within a range not losing the effects of the present invention. You can also. Furthermore, the antibacterial agent of the present invention can be used in the form of powder by evaporating and drying the extraction solvent from the above extract, or a solvent such as water, ethanol, propylene glycol, 1,3-butylene glycol, glycerin, etc. It can also be used after redissolving in Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.

The skin resident bacteria in the present invention mean bacteria growing on healthy human skin, and abnormal growth and reduction of these skin resident bacteria have various effects on the skin. Normalization is to improve the condition of the skin surface by normalizing the balance of the normal bacterial flora by inhibiting the growth of acne, the acne pathogen, and not the growth of aerobic bacteria. . The normal skin bacteria normalizing agent selectively suppresses the growth of acne bacteria and does not suppress the growth of aerobic bacteria, thereby normalizing the balance of normal skin flora and improving the surface condition of the skin. It is used in the sense of an external preparation for skin or a cosmetic containing an active ingredient that can be used. Here, acne bacteria are anaerobic bacteria that grow in human hair follicles, the scientific name is Propionibacterium acnes, and are the main detection bacteria when isolated and cultured from acne patient rashes. Other anaerobic bacteria detected on the skin are Propionibacterium
granulosum, etc., but P. acnes is a major factor in the development of acne, and is particularly effective in the present invention. Aerobic bacteria are bacteria that inhabit human skin, and Staphylococcus epidermidis.
Epidermidis, Staphylococcus aureus, Staphylococcus aureus, Gram-positive gonococcal Corynebacterium spp, etc. are raised, but the main bacteria detected from the periphery of human hair follicles are St.
Since it is aureus or St. epidermidis, it is effective against St. aureus or St. epidermidis in the present invention.

The normal skin bacterium normalizing agent of the present invention is characterized by comprising an aurene extract as an active ingredient, and containing this suppresses the growth of acne bacteria as anaerobic bacteria, and aerobic bacteria growth. It is characterized by maintaining. In this way, it is characterized by acting only on acne bacteria, and unlike conventional fungicides such as ibuprofen picool, it is useful because it has little effect on other skin resident bacteria.

In the present invention, the normal skin bacterium normalizing agent is characterized by containing an aurene extract. That is, it is not necessary to use a known anti-acne component or the like, and normalization of skin resident bacteria is possible by using an aurene extract.

The normal skin bacterium normalizing method of the present invention is characterized by suppressing the growth of acne bacteria on the skin and maintaining the growth of aerobic bacteria with the auren extract. That is, abnormal growth and reduction of skin resident bacteria that are bacteria growing on healthy human skin have various effects on the skin. Therefore, by using a normal skin bacterium normalizing agent that selectively suppresses the growth of acne bacteria and does not suppress the growth of aerobic bacteria, it suppresses the growth of acne bacteria of the acne pathogen on the skin, aerobic This is a method for improving the condition of the skin surface by normalizing the balance of normal skin flora by not inhibiting the growth of bacteria.

Furthermore, the present invention is also characterized by a method for producing a skin external preparation for normalizing skin resident bacteria or a cosmetic. This suppresses the growth of acne bacteria and does not inhibit the growth of aerobic bacteria. A method for producing an external preparation for skin or a cosmetic can be obtained. The auren extract can be used as it is as the skin external preparation or cosmetic of the present invention as it is, and it can also be blended during the production process of the skin external preparation or cosmetic.

The above-mentioned present invention can contain the following optional components according to the dosage form and application to such an extent that the effect is not hindered. That is, surfactants, water components such as water, ethanol, polyhydric alcohol, and the like, oils such as higher alcohols, liquid oils, silicone oils, petroleum jelly, oils and fats, powders, Thickeners, pigments, dyes, fragrances, antibacterial and antifungal agents, ultraviolet absorbers, vitamins, pH adjusters and the like can be blended.

Examples of the use of the external skin preparation for normalizing skin resident bacteria of the present invention include an external solution, a gel for external use, a cream, an ointment, a liniment, a lotion, a hap, a plaster, a spray, an aerosol, and the like Law.

The cosmetics for normalizing skin resident bacteria of the present invention include skin care such as lotion, milky lotion, cream, eye cream, serum, massage fee, pack fee, hand cream, body cream, sunscreen cosmetics, etc. Examples thereof include makeup cosmetics such as cosmetics, powder foundations, liquid foundations, cosmetic foundation cosmetics, white powder, concealer, and eye shadow.

Further, when the above-mentioned external preparation for skin and cosmetics are emulsion compositions, any of O / W type, W / O type, multilayer type such as O / W / O type, W / O / W type, etc. is possible. It is. Moreover, the kind of component which can be used when manufacturing these dosage forms, and its compounding quantity can be adjusted in the range which an expert can determine suitably according to a conventional means. These types and blending amounts are not limited to the examples shown below, and any component known to be able to produce the target dosage form and any blending ratio thereof can be used. .

As described above, the normal skin bacterium normalizing agent of the present invention is suitably used as an external preparation for skin or a cosmetic, and the product of the present invention can be used widely because it can normalize the normal skin bacterium. There are methods to use by hand and fingers, but other methods of use include, for example, a method of impregnating non-woven fabric, etc., wiping type sheet cleanser, sheet cleansing fee, sheet wet napkin and nasal drops, It can also be used for ear drops.

Examples are given below to make the present invention more specific. In addition, this invention is not limited by these Examples. First, various plant extracts as samples, test bacteria, and a method for culturing the bacteria will be described.

1. Plant extract The plant extract shown in Table 1 was used. Production examples will be shown below, but the present invention is not limited thereto.
(Note 1)

Ouren extract (manufacturing method) Ouren extract was obtained by adding 100 mL of 90 vol% hydrous ethyl alcohol to 10 g of Ouren or other plants of the same genus, followed by extraction at room temperature for 7 to 10 days, followed by filtration. At this time, the dry solid content of the aurene extract was 0.5%.
(Note 2)
Nettle extract (manufacturing method) The nettle extract was obtained by adding 50 mL of 50 vol% 1,3-butylene glycol to 10 g of nettle leaves, extracting at room temperature for 7 to 10 days, and then filtering. At this time, the dry solid content of the nettle extract was 1.2%.
(Note 3)
Wallet extract (Manufacturing method) Wallet extract was obtained by adding 200 mL of 50 vol% 1,3-butylene glycol to 10 g of the roots and rhizomes, extracting at room temperature for 7 to 10 days, and then filtering. . At this time, the dry solid content of the extract was 0.8%.
(Note 4)
Aloe leaf extract (manufacturing method) The aloe extract is prepared by dissolving the powder obtained by removing aloin from the juice obtained from the aloe leaves in purified water so that the volume is 0.6 Vol%. After standing for 10 days, it was obtained by filtration. At this time, the dry solid content of the aloe extract was 0.6%.
(Note 5)
Docami extract (manufacturing method) The docami extract was obtained by adding 100 mL of 50 vol% 1,3-butylene glycol to 10 g of the above-ground part of the flowering stage of Docami, followed by extraction at room temperature for 7 days, followed by filtration. . At this time, the dry solid content of the Dokudami extract was 0.8%.
(Note 6)
Tea leaf extract (manufacturing method) The tea leaf extract is steamed, steamed, dried, and minced with 10 ml of chanoki leaves, added with 100 mL of 50 vol% hydrous ethyl alcohol, soaked and frozen. The insoluble material was removed, 60% 1,3-butylene glycol was added, and the mixture was filtered. At this time, the dry solid content of the tea leaf extract was 1.79%.
(Note 7)
Neubara Fruit Extract (Manufacturing Method) Neubara fruit extract was prepared by adding 50 mL of 50 vol% hydrous ethyl alcohol to 10 g of Neubara fruit, performing reflux extraction for 5 hours twice, drying under reduced pressure, and 50% hydrous ethyl. Alcohol was added and extraction was performed at room temperature for 7 to 10 days, followed by filtration. At this time, the dry solid content of the Neubara fruit extract was 8.5%.
(Note 8)
Magwa root bark extract (manufacturing method) Magwa root bark extract was added to 10 g of mug root bark with 50 mL of 90 vol% hydrous ethyl alcohol, heated and extracted, dried under reduced pressure, and added with 50% hydrous ethyl alcohol. Extraction was performed at room temperature for 7 to 10 days, followed by filtration. At this time, the dry solid content of the Magwa root bark extract was 1.4%.
(Note 9)
European birch seed coat extract (manufacturing method) The European birch extract is 10 g of European birch bark, 50 ml of 50 vol% 1,3-butylene glycol is added and heated to extract the filtrate at room temperature for 7 to 10 days. Obtained by filtration. At this time, the dry solid content of the European birch extract was 0.8%.
(Note 10)
Onionis extract (manufacturing method) The onionis extract was prepared by adding 50 vol% 1,3-butyleneglycol 50 mL to 10 g of Ononis root, heating and extracting, and then allowing the filtrate to stand at room temperature for 7 to 10 days and filtering. I got it. At this time, the dry solid content of the onionis extract was 1.0%.
(Note 11)
Butcher Bloom Root Extract (Production Method) The Butcher Bloom extract was prepared by adding 100 mL of 70 vol% hydrous ethyl alcohol to 10 g of rhizome of Nagiida (butcher bloom), heating and extracting, drying under reduced pressure, and 50% hydrous ethyl alcohol. And extracted at room temperature for 7 to 10 days, followed by filtration. At this time, the dry solid content of the butcher bloom extract was 0.8%.
(Note 12)
Coffee seed extract (manufacturing method) The coffee seed extract is extracted by adding 100 mL of ethyl alcohol to 10 g of Arabica coffee tree or other related plant, filtered and dried under reduced pressure to obtain 10 g of coffee extract. A coffee extract was obtained by dissolving in 30% 1,3-butylene glycol. At this time, the dry solid content of the coffee seed extract was 1.96%.

2. Test bacteria Acne (anaerobic); Propionibacterium acnes ATCC 6919
Aerobic bacteria: Staphylococcus aureus IFO 13276, Staphylococcus epidermidis JCM
2414T (Staphylococcus epidermidis JCM 2414T)

3. Bacteria culture method [Acne bacteria culture method]
A solution in which acne bacteria is dispersed in a sterile physiological saline solution at about 10 cfu / ml is prepared in advance, and 0.05 ml of this solution is uniformly inoculated on the surface of the GAM agar medium and air-dried. Sterile circular filter paper with a diameter of 8 mm is impregnated with 0. 5 ml of 10 vol% aqueous solution of candidate substances such as uren extract and other plant extracts, placed on the GAM agar medium inoculated with the bacteria, and anaerobic container And put Aneropac Kenki (Mitsubishi Gas Chemical Co., Ltd.), covered and cultured at 37 ° C. for one week.
[Method of culturing aerobic bacteria]
Prepare a solution in which St. aureus and St. epidermidis, which are aerobic bacteria, are dispersed in advance in sterile physiological saline so that the concentration is about 10 cfu / ml, and 0.05 ml of this solution is uniformly applied to the surface of the SCD agar medium. Inoculate and let dry naturally. Sterile 8 mm diameter circular filter paper is impregnated with 0.05 ml of a 10 vol% aqueous solution of candidate substances such as auren extract and other plant extracts, and placed on the SCD agar medium inoculated with the fungus at 32.5 ° C. Cultured for one week.

Then, the test method performed about the microbial growth inhibitory effect of the various plant extracts by the inhibition circle method, and the evaluation method of the microbial growth inhibitory effect are demonstrated.

4). Test Method A circular filter paper having a diameter of 8 mm impregnated with various plant extracts was placed in a medium inoculated with bacteria and cultured in a constant temperature sterilization environment for one week.

5). Evaluation method of growth inhibitory effect (Evaluation method of growth inhibitory effect of acne)
After culturing the acne bacteria at 37 ° C. for one week, by measuring the size of the bacteria non-growth zone (growth inhibition zone, transparent zone) generated around the circular filter paper impregnated with various plant extracts, The growth inhibitory effect of the candidate substances on the bacteria was evaluated. The evaluation criteria are as follows.
[Evaluation criteria for growth inhibitory effect]
Δ: Growth suppression zone 8 mm or less ○: Growth suppression zone greater than 8 mm and 15 mm or less ◎: Growth suppression zone greater than 15 mm [Evaluation method for aerobic bacterial growth inhibition effect]
Measure the size of the non-growth zone (growth inhibition zone, transparent zone) generated around the circular filter paper impregnated with various plant extracts after culturing the aerobic bacteria at 32.5 ° C for one week. Thus, the growth inhibitory effect of each candidate substance on the bacteria was evaluated.
The evaluation criteria are as follows.
The evaluation criteria are as follows.
[Evaluation criteria for growth inhibitory effect]
◎: Growth suppression zone 8 mm or less ○: Growth suppression zone greater than 8 mm and 15 mm or less Δ: Growth suppression zone greater than 15 mm

From the results in Table 1, the auren extract showed an excellent antibacterial effect against acne bacteria, and showed a low antibacterial effect against any aerobic bacteria. It turned out that it is excellent in the conversion effect. On the other hand, any plant extract other than the auren extract had no excellent bactericidal effect against acne.

The lotion of Example 2 is an example in which bacteria were collected from human skin and used as an example of an external skin preparation or cosmetic as an experimental method for evaluating the effect of normalizing skin resident bacteria. It was prepared by the formulation shown in Table 2 and the following production method.

(Note 13)
Parsley extract (manufacturing method) Parsley extract is obtained by adding 120 g of 1,3-butylene glycol to 10 g of Dutch seri (parsley) leaves, extracting and filtering at room temperature for 10 days with stirring, and adding 10 g of parsley extract. obtain. A parsley extract was obtained by dissolving in 30% 1,3-butylene glycol. At this time, the dry solid content of the parsley extract was 0.5%.

(Manufacturing method)
A: Component (3) to component (5) are mixed.
B: Component (1), component (2), component (6) to component (13) are mixed.
C: A was mixed with B to obtain a skin lotion.

[Method for collecting skin resident bacteria]
After 1 hour of face washing, the subject applied 1 ml of 2cm square of lotion containing each candidate substance to the subject and 1cm square in the test application site immediately after application and 1 hour after application. The skin resident bacteria were collected by rubbing with a sterile cotton swab for 1 minute, taken up in 10 g of 0.065M phosphate buffer, and collected by vortexing for 5 minutes.

Method for culturing bacteria on human skin [Method for culturing acne bacteria]
The suspension obtained by the above method was appropriately diluted with 0.065M phosphate buffer. The diluted solution was separated on a GAM agar medium cooled to 50 ° C. after sterilization, mixed well, and allowed to stand to solidify the agar medium. Then, it put in the anaerobic container, put Aneropack Kenki (Mitsubishi Gas Chemical Co., Ltd.), covered, and cultured at 37 degreeC for one week.
[Method of culturing aerobic bacteria]
The suspension obtained by the above method was appropriately diluted with 0.065M phosphate buffer. After the sterilization, the diluted fungus on the skin prepared by the above method was separated on a cooled and solidified SCD agar medium, dried, and cultured at 32.5 ° C. for one week.

[Method of evaluating bacterial growth inhibition]
The number of colonies appearing on various media was counted, and changes in acne bacteria or aerobic bacteria in each candidate substance were calculated from the following calculation formulas and evaluated according to the following evaluation criteria.
Change amount = A / B
A: Acne bacteria or aerobic bacteria collected from the site where the skin lotion containing the candidate substance is applied B: Acne bacteria or aerobic bacteria collected from the site where the skin lotion not containing the candidate substance is applied

[Evaluation criteria for growth inhibitory effect]
++: Change amount less than 0.01 +: Change amount of 0.01 or more and less than 1-: Change amount of 1 or more [Standard for normalization of skin resident bacteria]
○: Acne bacteria inhibitory effect is ++ and aerobic bacteria inhibitory effect is − ×: Acne bacteria inhibitory effect is − or ++

From the results shown in Table 2, it was found that in either of the products 1 or 2 of the present invention, the auren extract exhibits a superior effect as a normalizing agent for skin resident bacteria. On the other hand, comparative product 1 and comparative product 2 containing a parsley extract instead of an auren extract were suggested to have a selective antibacterial effect by primary screening. No effect on acne was observed. It was found that all of the comparative products 3 containing no plant extract were not excellent in the inhibitory effect against acne bacteria and did not have an antibacterial effect against aerobic bacteria. In Comparative Product 4 containing ethanol in Comparative Product 3, the antibacterial effect of acne bacteria was low, and the antibacterial effect of aerobic bacteria was very high. Furthermore, the comparative product 5 containing ibuprofen piconol also had a low antibacterial effect against acne bacteria, and an antibacterial effect against aerobic bacteria was observed.
From the above results, it can be said that the aurene extract exerted a strong antibacterial effect only on acne bacteria without affecting the growth of aerobic bacteria on the skin. The effect of the present invention makes it difficult for the skin barrier function to decrease, and it can also be expected as an effect of suppressing skin roughness.

Claims (14)

  A normalizing agent for skin resident bacteria containing an aurene extract as an active ingredient.   2. The normal skin bacterium normalizing agent according to claim 1, wherein normalization of the skin resident bacteria suppresses the growth of acne bacteria and maintains the growth of aerobic bacteria. Acne bacteria
The normalizing agent for skin resident bacteria according to claim 1 or 2, which is acnes. Aerobic bacteria
The normalizing agent for skin resident bacteria according to any one of claims 1 to 3, which is aureus or St. epidermidis.   A skin external preparation or cosmetic comprising the skin resident normalizing agent according to any one of claims 1 to 4.   A method for normalizing skin resident bacteria, characterized by suppressing the growth of acne bacteria in the skin and maintaining the growth of aerobic bacteria with an auren extract. Acne bacteria
The method for normalizing skin resident bacteria according to claim 6, which is acnes. Aerobic bacteria
It is aureus or St. epidermidis, The skin normal bacteria normalization method of Claim 6 or 7 characterized by the above-mentioned. The method for normalizing skin resident bacteria according to any one of claims 6 to 8, wherein an extract of aurene is used. A method for producing a skin external preparation or cosmetic for normalization of skin resident bacteria, characterized by comprising an oren extract as an active ingredient and added to the skin external preparation or cosmetic. The preparation of a skin external preparation or cosmetic for normalization of skin resident bacteria according to claim 10, wherein normalization of the skin resident bacteria suppresses the growth of acne bacteria and maintains the growth of aerobic bacteria. Method. Acne bacteria
The method for producing a skin external preparation or cosmetic for normalization of the resident skin bacterium according to claim 10 or 11, which is acnes. Aerobic bacteria
It is aureus or St. epidermidis, The manufacturing method of the skin external preparation or cosmetics for skin resident normalization of any one of Claims 10-12 characterized by the above-mentioned. The method for producing a skin external preparation or cosmetic for normalizing resident skin bacterium according to any one of claims 10 to 13, characterized by using an aurene extract.