JPH06279256A - Skin external preparation - Google Patents

Abstract

PURPOSE:To obtain a skin external preparation capable of keeping the balance of dermal indigenous bacteria, preventing common acne and skin roughening and aiming at the maintenance of the healthy skin by bending weakly acidic galenical extracts having antimicrobial activity against the dermal indigenous bacteria. CONSTITUTION:This skin external preparation comprises at least one galenical extract selected from Hydrangeae Dulcis Folium, Sanguisorbae Rhizoma, Sophorae Radix, Sophorae Flos, Schizandrae Fructus, Phellodendri Cortex, Coptidis Rhizoma, Pseudocydoniae Fructus, Rosae Fructus, Prunellae Spica, Paeoniae Radix, Saposhnikoviae Radix and Moutan Cortex.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention has a specific antibacterial action against mild skin acidic bacteria under mildly acidic conditions in order to maintain the balance of normal bacterial skin and maintain healthy skin. The present invention relates to a skin external preparation containing a crude drug extract.

[0002]

2. Description of the Related Art Generally, it is said that various kinds of microorganisms are resident on human skin to form a microflora, which is one of the factors for maintaining the homeostasis of the skin. The skin-resident microorganisms are mainly Propionibacterium acnes (Prop
Propionibacterium, such as Ionibacterium, and Staphylococcus aureus called Staphylococcus aureus
cus aureus) and Staphylococcus epidermidis called Staphylococcus epidermidis (Sta
There are Staphylococcus bacteria such as phylococcus epidermidis) and various other microorganisms such as Micrococcus bacteria. While these microorganisms act as a barrier on the skin, it is known that excessive proliferation causes skin diseases such as acne and inflammation.

Accumulation of sebum occurs on the skin due to increased sebum secretion, and the number of microorganisms resident in the skin increases. It is said that due to the increase in bacterial lipase accompanying this, triglyceride which is a sebum component is decomposed and free fatty acids increase to cause inflammation. The lipase that functions in this way is Propionibacterium acnes, which is an anaerobic bacterium.
s) but also Staphylococcus epidermidis, a coagulase-negative staphylococcus
ermidis) is also known to exist,
(Skin, 10,585,1969) It has also been shown to degrade triglycerides. (J. Inves
t. derm. 53,1,1969)

Conventionally, many drugs have been used for the purpose of suppressing the growth of such microorganisms that induce skin inflammation. For example, tetracycline, penicillin, erythromycin, chloramphenicol, and other antibiotics, bactericides, and antibacterial agents are known, but these have side effects and suppress even useful microorganisms on the skin, and they are commonly used in the skin. It has been pointed out that it sometimes causes an unfavorable situation from the viewpoint of so-called ecology, which destroys the delicate balance of the presence of bacteria.

In recent years, coagulase-negative staphylococci represented by Staphylococcus aureus and Staphylococcus epidermidis, which have been considered as non-pathogenic indigenous bacteria, are involved in the aspect of attenuated bacterium infection and atopic dermatitis. From the aspect of the above, attention has been paid to research on the bacterial flora in many cases. In addition, there have been some reports on the healthy skin flora, and the relationship between the distribution of healthy skin indigenous bacteria and the skin quality has been clarified, and the necessity of controlling skin microorganisms has been emphasized.

[0006]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention As a result of detailed examination of healthy skin flora, the number of Staphylococcus epidermidis, which accounts for all or most of aerobic bacteria in healthy beautiful skin, has been found. , It was found that there was little fluctuation throughout the day and a constant bacterial count was maintained.

[0007] Therefore, the inventors of the present invention have thought that it may contribute to the prevention and improvement of skin roughness by keeping the number of bacteria on the skin constant, and as a result, have conducted extensive studies and as a result, have weakly acidic and antibacterial properties. It was found that the continuous use of the herbal medicine extract effectively controls the number of skin indigenous bacteria, and the present invention has been completed.

[0008]

[Means for Solving the Problems] That is, the present invention provides an external preparation for skin, which comprises, as an active ingredient, one or more kinds of extracts obtained from the following plants having bactericidal and antibacterial activities in a weakly acidic region. It is about.

Plants having a bactericidal and antibacterial action in the weakly acidic region used in the present invention are (1) Amanda Hydrangeae Dulcis
Folium (2) Jiang Sanguisorbae Rhiz
oma (3) Sophorae Radix (4) Sophora Flos (5) Sophorae Frost (5) Gomiko Schisandrae Fruct
us (6) Karin Pseudocydoniae Fr
uctus (7) Yomi fruit Rosae Fructus (8) Summer hay Prunellae Spica (9) Peony radish Paeoniae Radix (10) Windbreak Saposhnikoviae Ra
diox (11) peony skin Moutan Cortex (12) yellow oak Phellodendri Court
ex (13) Huanglian There are 13 types of Coptidis Rhizoma.

These crude drug extracts used in the present invention have antibacterial properties under mildly acidic conditions, and when applied on the skin, the number of microbes on the skin is controlled to control the number and distribution of skin-resident bacteria. It is possible to improve the skin roughness and acne caused by the condition and keep the skin in a healthy condition.

[0011] These extracts have different antibacterial activity depending on the type of bacteria acting, and the antibacterial activity of some of these extracts is influenced by pH. Therefore, by selecting and using these extracts, it is possible to deal with individual differences in skin bacterial flora, and therefore, it is possible to effectively apply them in accordance with the skin quality and skin condition of an individual.

In the present invention, the herbal medicine of the plant, that is, the whole or part of the plant (eg, whole plant, leaf, root, rhizome, stem, root bark, flower) is simply processed (eg, dried,
Cut, powdered) or its extract is used.

The extract used in the present invention may be an as-extracted solution, an extract obtained by concentrating the solvent, or a powder obtained by distilling the solvent or a viscous substance, Also, a diluted solution thereof can be used.

As the method for producing the herbal medicine extract used in the present invention, the above-mentioned plant or herbal medicine dried product is treated with water or an organic solvent (petroleum ether, cyclohexane, carbon tetrachloride, toluene, benzene, dichloromethane, chloroform, ether, ethyl acetate, Butanol, acetone, n-propanol, ethanol, methanol, pyridine, polyethylene glycol, propylene glycol, butylene glycol, etc.) or a solvent in which these are mixed at a constant ratio, for example, hydrous alcohol or the like is used. Preferably,
Ethanol, water, benzene, butylene glycol, hydroalcohol and the like are preferable. The extraction conditions are not particularly limited as long as they are generally used for plant extraction.

The dosage form of the external preparation for skin of the present invention is arbitrary,
For example, a dosage form such as lotion, cream, emulsion, pack, or the like can be used, and can be blended in any amount at any stage of the production process. The external preparation for skin of the present invention includes, in addition to the above-mentioned essential components, various components generally used in cosmetics, quasi-drugs, pharmaceuticals, etc., and an aqueous component within a range that does not impair the effects of the present invention as necessary. An oil component, a moisturizer, a thickener, an antiseptic, an antioxidant, a fragrance, a coloring agent, a drug and the like can be added. The blending amount is 0.005 as the dry residue amount.
It is desirable to contain 10% by weight, and more preferably 0.05% to 5% by weight is suitable because it shows a remarkable effect on bacteria on the skin.

[0016]

The external preparation for skin of the present invention is effective for the prevention, treatment and treatment of rough skin or acne.

The present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the examples shown below. In addition, in the following examples, the compounding amount is shown by weight%.

(Production Example 1) The dried powder of each crude drug was extracted with 10 volumes of ethanol at room temperature for 7 days, filtered and concentrated under reduced pressure to remove ethanol to obtain an extract.

(Production Example 2) The dried powder of each crude drug was extracted with 5-fold amount of 1,3-butylene grelicol and then filtered to obtain an extract.

Example 1 The antibacterial activity of each crude drug extract against various bacteria under mildly acidic conditions was measured by an agar plate diffusion method using a heart infusion agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.). The extract of Production Example 1 was made 10-fold volume with ethanol, and 0.05 ml thereof was soaked in a filter paper disk having a diameter of 8 mm, and closely contacted with the agar plate on which the test bacterium was inoculated and dispersed.
It was cultured at 30 ° C. for 24 hours. At the end of culturing, the diameter of the transparent zone (growth inhibition zone) in which the growth of bacteria around the filter paper was inhibited was determined.

The results are shown in Table 1. Two Staphylococcus strains have a pH of 5.5, and Micrococcus luteus has a pH of 6.
The size of the inhibition circle on medium 0 is shown.

[0021]

[Table 1]

Example 2 The growth inhibition rate of each extract against Staphylococcus epidermidis was measured. To a heart infusion medium (manufactured by Nissui Pharmaceutical Co., Ltd.) adjusted to pH 5.5 and pH 7.2, the diluted solution of the crude drug ethanol extract used in Example 1 was added to 0.2%, and Staphylococcus epidermidis was added. AT
After inoculating CC 14990 and culturing at 30 ° C. for 24 hours, the viable cell count was measured. The growth inhibition rate was calculated by the following formula. The results are shown in Table 2.

[0023]

[Equation 1]

[0024]

[Table 2]

(Example 3) Lotion

[0026]

[Table 3]

To the component (1), (4), (5) and (6) were mixed and dissolved at room temperature, and (2), (3) and (7) were added with stirring to obtain a lotion.

(Example 4) Cream

[0029]

[Table 4]

The above components (2) to (9) are mixed and heated to 7
Set to 5 ° C. In addition, the above prescriptions (1) and (10) to (1
2) was heated in the same manner and heated to 75 ° C., and the mixture was uniformly emulsified with a homomixer to obtain an O / W cream.

(Example 5) Pack

[0032]

[Table 5]

After adding (4), (5) and (7) to the above component (9) with stirring and dissolving, (3) was added and heated to 70 ° C. to dissolve. Next, (1), (2) and (8) were added to and dissolved in (6), and the mixture was cooled to obtain a pack.

Example 6 Emulsion

[0035]

[Table 6]

The above components (1) to (8) were mixed and heated and dissolved at 70 ° C. After mixing (11) to (12),
The mixture was heated to 70 ° C. and homogenized with a homomixer. This was dissolved in (9) with stirring (1
0) to (13) and (14) were added, and the mixture was cooled to 30 ° C. with good mixing to obtain an emulsion.

Example 7 The effect of continuous application of the product of the present invention on microorganisms on the skin was measured by the following method. After washing the face of four test subjects, 1 g of the lotion shown in Example 3 was uniformly sprayed on the right cheek, and the lotion of Example 3 was not added to the lotion of Example 3 on the left cheek (comparative example). ) Was similarly sprayed. After that, I had a daily life, and on the next day, swab 1 cm on the cheek.
Bacteria were collected from all sides, and the number of general viable bacteria, the number of salt-tolerant microorganisms,
And the number of anaerobic bacteria was measured and set as the first day. After collecting the bacteria, the face was washed, and then lotion was sprayed in the same manner, and the process of collecting the bacteria the next day was performed until the 8th day, and the fluctuation of the viable cell count was measured.

FIG. 1 shows the results when the lotion of Example 3 was applied, and FIG. 2 shows the results of the comparative example. On the cheeks to which the lotion containing the extract was applied, the viable cell count did not fluctuate with the passage of days, and individual differences were also small. Regarding the bacterial species, the number of salt-tolerant bacteria such as Staphylococcus spp. And Micrococcus spp. Has become large in the aerobic bacteria, and the difference in the number of aerobic and anaerobic bacteria has occurred. Is small, and the maintenance of the indigenous bacterial ecosystem is strongly maintained.

[Brief description of drawings]

FIG. 1 is a graph showing changes in the number of bacteria on human skin when continuous application of the lotion shown in Example 3 is performed.

FIG. 2 is a graph of a comparative example with respect to FIG. That is, it is a graph showing changes in the number of bacteria on human skin when the lotion shown in Example 3 was applied with lotion without adding a herbal medicine extract.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Internal reference number FI Technical display location A61K 35/78 J 7822-4C K 7822-4C F 7822-4C N 7822-4C ADA C 7822-4C ADZ H 7822-4C

Claims (1)

[Claims] 1. One or more kinds of crude drug extracts selected from sweet green tea, ground peony, bitter ginseng, Sophora, schizandra, yellow oak, yellow ream, quince, corn, summer hay, peony, windbreak, peony skin. A skin external preparation characterized by containing.