KR102381251B1 - Cosmetic composition for anti-oxidation, skin moisturizing, or improving of acne skin or skin trouble containing complex extract of Eriobotryae Folium - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an Eriobotryae folium complex extract. More specifically, the present invention relates to a cosmetic composition comprising complex extracts of Eriobotryae folium, dictamnus root, lappa fruit, Salicis radicis cortex and Pueraria lobata, thereby being effective in alleviating acne skin and skin trouble. The Eriobotryae Folium complex extract according to the present invention exhibits excellent antibacterial activity against a Propionibacterium acnes strain, a Staphylococcus epidermidis stain, and a Staphylococcus aureus strain, which are bacteria causing acne, as well as excellent activity of inhibiting nitric oxide (NO) production. Accordingly, the Eriobotryae Folium complex extract not only exhibits remarkable acne skin and skin trouble alleviating effects, but also exhibits excellent antioxidant and skin moisturizing effects. Therefore, the Eriobotryae Folium complex extract can be usefully employed in products such as quasi-drugs and health functional foods as well as cosmetics for alleviating acne skin and skin trouble.

Description

TECHNICAL FIELD A cosmetic composition for anti-oxidation, skin moisturizing, improving skin acne, or improving skin troubles, comprising a bivalve leaf complex extract as an active ingredient. Eriobotryae Folium}

The present invention relates to a cosmetic composition comprising a complex extract of Eriobotryae Folium . Specifically, it relates to a cosmetic composition having antioxidant, skin moisturizing, skin acne improvement and skin trouble improvement effects by including the bipayeop, tinea skin, woobangja, eugeunpi and galgeun complex extract as active ingredients.

Recently, interest in cosmetics derived from natural products is increasing. In addition, as the demand for functional cosmetics increases, there is a problem in that the protective ability or regeneration ability of the skin is rather reduced due to various chemical ingredients used as functional ingredients. Accordingly, it is becoming fashionable to make and use cosmetics made of natural materials, from skins, lotions, to color cosmetics. In the case of people with skin diseases such as atopic dermatitis or sensitive skin, interest in cosmetics derived from natural products with excellent skin improvement activity while minimizing skin irritation is increasing.

Loquat ( Eriobotrya japonica ) is an evergreen tree belonging to the Rosaceae family, and its fruits, roots, stem bark and leaves are used medicinally in oriental medicine. In particular, bipayeop is known to have the effect of clearing the lungs, harmonizing the stomach, lowering the qi, and clearing the phlegm (Chinese Medicine Dictionary). In addition, it is effective for skin diseases related to inflammation, cough, sputum, etc., and pharmacological activities such as lowering blood sugar, improving liver function and various anti-inflammatory activities have been reported (Nishioka et al ., Biol. Pharm. Bull., 25 : 1053-1057, 2002). Loquat leaves contain a large amount of various triterpenoid compounds including ursolic acid, oleanolic acid, maslinic acid and tomentic acid (Taniguchi et al. al., Phytochemistry, 59: 315-323 (2002)), as well as sesquiterpene glycosides, flavonoid glycosides, and various polyphenols are reported to be contained (Shimizu et al . , Chem . Pharm. Bull., 34: 2614-2617 (1986); de Tommaasi et al. , J. Nat. Prod., 55: 1025-1032, 1067-1073 (1992); Kawahara et al ., Chem Pharm. Bull., 50: 1619-1620 (2002)).

Conventionally, due to its skin improvement activity, bipa leaf has been studied as a raw material for antioxidant, skin moisturizing, or wrinkle improvement cosmetics.

Korean Patent Registration No. 10-0684776 discloses that the extract of bipayeop has skin wrinkle improvement activity, and Korean Patent Publication No. 10-2020-0108207 discloses that the fat-soluble extract of bipayeop and pogongyeong has skin moisturizing activity. This is disclosed.

The present inventors have conducted research on natural products that are non-toxic and have excellent skin condition improvement effects, and when bivalve leaf is used as a complex extract with tinea dermatophyllum, chrysanthemum rhododendron, Eugeun derma and ginseng root, the effect of improving skin acne and improving skin troubles is very good. , has completed the present invention by discovering that it exhibits excellent antioxidant and skin moisturizing effects.

KR 10-0684776 B KR 10-2020-0108207 A

An object to be solved by the present invention is to provide a cosmetic composition for antioxidation, skin moisturizing, improving skin acne, or improving skin troubles, which contains a complex extract of bipayop, tinea skin, woobangja, eugeunpi, and galgeun as active ingredients.

Another object of the present invention is to provide a composition for external application for anti-oxidation, skin moisturizing, improving skin acne or improving skin troubles, comprising the above-mentioned bipayeop, tinea skin, woobangja, eugeunpi, and galgeun complex extract as active ingredients.

Another object of the present invention is to provide a food composition for antioxidant, skin moisturizing, skin acne improvement or skin trouble improvement comprising the above-mentioned bipayeop, tinea skin, woobangja, eugeunpi and galgeun complex extract as active ingredients.

In order to achieve the above object, the present invention provides a cosmetic composition for anti-oxidation, skin moisturizing, skin acne improvement or skin trouble improvement, comprising a bipayeop, tinea skin, woobangja, eugeunpi and galgeun complex extract as active ingredients.

According to an embodiment of the present invention, the complex extract may be obtained by extracting bipayeop, baekseonpi, woobangja, eugeunpi, and brown root with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

According to an embodiment of the present invention, the composition may have a nitric oxide (NO) production inhibitory activity.

According to one embodiment of the present invention, the composition is Propionibacterium acnes ( Propionibacterium acnes ) strain, Staphylococcus epidermidis ( Staphylococcus epidermidis ) strain, and Staphylococcus aureus ( Staphylococcus aureus ) for the strain It may have antibacterial activity.

According to an embodiment of the present invention, the complex extract contains a bivalve leaf extract, tinea skin extract, lavender extract, Eugeunpi extract, and black root extract in a weight ratio of 1: 0.5-2.0: 0.1-1.5: 0.05-1.0: 0.05-1.0 by weight. it may have been

According to an embodiment of the present invention, the complex extract may be 0.001 to 10% by weight based on the total weight of the composition.

According to an embodiment of the present invention, the composition may further include 10 to 30 parts by weight of lavender extract based on 100 parts by weight of the complex extract.

According to one embodiment of the present invention, the cosmetic composition may be a formulation selected from lotion, emulsion, cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, and makeup remover.

In addition, the present invention provides a composition for external application for anti-oxidation, skin moisturizing, skin acne improvement or skin trouble improvement, comprising the complex extract of bipayeop, tinea skin, woobangja, eugeunpi, and galgeun as active ingredients.

According to an embodiment of the present invention, the composition for external application for skin may be a formulation selected from ointments, patches, gels, creams and sprays.

In addition, the present invention provides a food composition for antioxidant, skin moisturizing, skin acne improvement, or skin trouble improvement, comprising a bipayeop, tinea skin, woobangja, eugeunpi, and galgeun complex extract as active ingredients.

The bipalea complex extract according to the present invention exhibits remarkable antibacterial activity against acne causative bacteria and nitric oxide (NO) production inhibitory activity, etc. indicates Therefore, the bipayeop complex extract can be usefully used in products such as quasi-drugs and health functional foods as well as cosmetics for improving skin acne and skin troubles.

Hereinafter, the present invention will be described in detail.

According to one embodiment of the present invention, the present invention provides a cosmetic composition for antioxidation, skin moisturizing, skin acne improvement or skin trouble improvement, comprising the extract of bipayeop, tinea skin, woobangja, eugeunpi, and rhubarb as active ingredients do.

As used herein, the term "composite extract" refers to a mixture of 1) biphaeop extract, tinea skin extract, eucalyptus extract, rhododendron extract, and black root extract, or 2) extracting the extract after first mixing pipayeop, tinea skin, woobangja, yew geunpi and black root. means form. As for the complex extract, it is more preferable in terms of antioxidant, skin moisturizing, skin acne improvement or skin trouble improvement efficacy to prepare each extract separately and then mix it as in No. 1) above.

In the present invention, "bipayeop complex extract" means "bipayeop, baekseonpi, woobangja, yugeunpi and galgeun complex extract".

The loquat leaf ( Eriobotryae Folium ) is a leaf portion of loquat (Eriobotrya japonica), an evergreen tree belonging to the family Rosaceae. In oriental medicine, fruits, roots, stem bark and leaves are used medicinally. Bipayeop is known to have the effect of clearing the lungs, harmonizing the stomach, strengthening the qi, and clearing the wall. Bipa leaf contains a lot of triterpenoid compounds, including ursolic acid, oleanolic acid, masrinic acid, and tometic acid, and has been reported to have antioxidant, anti-inflammatory, anti-HIV, anti-mutagenic and anti-cancer activity.

As used herein, the term "loquat leaf extract" refers to an extract obtained by extracting the leaves of a loquat tree.

The baekseonpi refers to the root bark of tinea ( Dictamnus dsycarpus Turez ), which is a perennial herb of the rutaceae family. The baekseonpi is the root of tinea in the fall, washed with water, peeled and dried. As ingredients, the root contains 0.13% of alkaloids, and the root bark contains 0.02 to 0.03% of dictamnin (dictamine), as well as skimmianin, triconeline, choline, dictamnolactone, praxinelon, dictamnolide, obaku. There are rice fields, saponins, campesterol, sitosterol, and essential oils. It is known as an important drug used for restless limb paralysis in oriental medicine, and is used as a parasitic drug and is used as a fever reducing drug, analgesic, antispasmodic, antispasmodic, sedative, It is used as a diuretic.

In the present invention, "tinea skin extract" refers to an extract obtained by extracting the root bark of ringworm.

The woobangja ( Arctii Fructus ) is a fruit of burdock ( Arctium luppa L. ) native to India belonging to the Asteraceae (Compositae), arctiin, arctigenin (arctigenin), iso-actigenin (isoarctigenin), etc. It contains and is known to exhibit blood sugar-lowering action (information and exposure, Dohaehyangyak Dictionary, Youngrimsa, pp.1010-1011, 1998).

In the present invention, the term "Ubangja extract" refers to an extract extracted from burdock ( Arctium lappa L. ) fruit.

The yugeunpi ( Ulmus pumila L. ) is the root bark of a species of bison in the genus Elm, and the scientific name of the tree is Ulmus pumila and is called Siberian elm . Yugeunpi has been widely used as a herbal medicine. Recently, there are studies on the anti-obesity and anti-cancer effects of Yugeunpi, and also studies related to skin such as improvement of atopic dermatitis and improvement of wrinkles caused by UV rays have been reported.

In the present invention, "Yugunpi extract" refers to an extract obtained by extracting the root bark of Bizul tree.

The kudzu root (arrowhead root, Pueraria radix) refers to the dried root or bark of the legume vine in Korea, China, and Japan, or the dried root after removing the bark of arrowroot (Pueraria montana var. lobata: also referred to as Pueraria lobata). The main chemical components are puerarin and daidzin, which are a kind of isoflavones. In some studies on cancer cells, it has been reported that the treatment of arrowroot extract or powder inhibited the proliferation of breast cancer cells and reduced the growth of breast cancer tumors. In addition, galgeun is used in the treatment of colds, high fever, headache, muscle pain, and stiffness in the back of the neck as a mechanism to release heat while relaxing muscles in oriental medicine. It is also used to raise yang and stop diarrhea. In addition, it is used to replenish the body's essence, quench thirst, and relieve alcohol poison (酒毒). In addition, it is used for dysentery, high blood pressure, heart-related diseases, diabetes, and cancer.

In the present invention, the term "Gargeun extract" refers to an extract extracted from the dried root of arrowroot.

In the present invention, the bipayeop, tinea skin, Ubangja, Yugeunpi, and black root are each or a mixture, in a weight ratio of 1: 10 to 30, preferably 1: 15 to 25, more preferably 1: 18 to 22, with the extraction solvent. It may be prepared by mixing and extracting at 65 to 95 ℃, preferably 70 to 90 ℃, more preferably 75 to 85 ℃ for 1 to 10 hours, preferably for 1 to 5 hours, followed by concentration under reduced pressure. . When the weight ratio is out of the above range, the active ingredients of bipayeop, baekseonpi, woobangja, yugeunpi and galgeun may be extracted in a small amount in the extract.

The extraction solvent for extracting each of the extracts is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The lower alcohol may include 20 to 80% methanol, ethanol, butanol or propanol.

The extraction solvent is not particularly limited, but the extract extracted with an aqueous solution of 60 to 80% ethanol preferably acts on the alleviation or improvement of skin acne or skin troubles.

The complex extract of bipayeop, baekseonpi, woobangja, eugeunpi and galgeun effectively inhibits the production of nitric oxide (NO), thereby exhibiting anti-inflammatory or trouble improving efficacy. In addition, the extract of bipayeop, tinea skin, sphagnum asiatica, rhododendron prickly pear, and black root extract of the present invention is a Propionibacterium acnes strain, a Staphylococcus epidermidis strain, and a Staphylococcus aureus ( Staphylococcus aureus ) Since it exhibits excellent antibacterial activity against the strain, it exhibits antibacterial or skin acne improving effect, and shows antioxidant or skin moisturizing effect. In addition, since the extract of the bivalve leaf complex of the present invention is a natural product-derived material, it is non-toxic and does not cause side effects even when applied to the human body, so it can be used as a cosmetic composition.

The inflammatory response is a mechanism to regenerate damage caused by physical action, harmful substances, chemical stimuli, bacterial infection, etc. as part of the body's defense response to external stimuli. The continuous inflammatory response rather causes mucosal damage and promotes skin tissue damage, inflammation, and skin troubles.

Macrophages play a very important role in the immune response and regulate various mediators of inflammation, including nitric oxide, prostaglandins, and inflammatory cytokines, which are immune proteins. Among them, nitric oxide plays an important role, such as killing bacteria or removing tumors, but excessive nitric oxide production by etiology causes inflammation, and such inflammation causes skin troubles.

That is, when the nitric oxide production of macrophages is suppressed, skin inflammation can be prevented, alleviated, improved and treated, and skin troubles caused by inflammatory reactions can be prevented, alleviated, improved and treated.

Specifically, in an embodiment of the present invention, it was confirmed that nitric oxide production in macrophages was inhibited compared to that using the basal medium in the case of using each of the bivalve leaf extract, tinea skin extract, woobangja extract, eugeunpi extract, and garlic root extract.

In particular, nitric oxide, which is superior to that of a single extract of each plant, by the synergistic or cooperative action between the extracts and/or the active ingredients in the extract, the extract of bipayeop, white ginseng, woobangja, eugeunpi, and galgeun complex extract of the present invention Compared with L-NMMA (L-NG-Monomethylarginine), which is a known nitric oxide production inhibitor, it has been confirmed that it has an equivalent and very strong nitric oxide production inhibitory ability in macrophages did

Specifically, the bipayeop, tinea skin, Ubangja, Eugeunpi, and bald eagle complex extract of the present invention further increases the ability to inhibit nitric oxide production than the single extract of bipayeop, tinea skin, woobangja, yewgeunpi or galgeun, resulting in better anti-inflammatory or skin trouble improvement. efficacy could be shown.

On the other hand, in the present invention, "acne (Acne vulgaris)" is an acne causative bacterium, Propionibacterium acnes ) strain, Staphylococcus epidermidis ) strain, or Staphylococcus aureus ( Staphylococcus aureus ) ) may be "bacterial infectious acne" caused by the proliferation of the strain.

When the proliferation of the Propionibacterium acnes strain, Staphylococcus epidermidis strain, or Staphylococcus aureus strain is inhibited, it occurs due to the proliferation of the acne causative bacteria It can relieve, improve and treat skin acne.

In particular, Propionibacterium acnes is anaerobic, Gram positive, and bacillus that live as symbiotic bacteria in the sebaceous gland unit. Abnormal follicular epithelium exfoliation causes anaerobic conditions in the hair follicles, promoting the proliferation of Propionibacterium acnes. In addition, Propionibacterium acnes metabolizes the glycerol component of the excessively secreted sebum triglyceride into free fatty acids by secreting a lipase and a chemotactic factor. These extra free fatty acids promote the production of inflammatory white blood cells around the hair follicles. On the other hand, inhibiting the proliferation of Propionibacterium acnes minimizes the rupture of the comedones and suppresses the development of acne.

Specifically, in one embodiment of the present invention, it was confirmed that the proliferation of acne-causing bacteria was inhibited compared to the use of the basic medium when each of the extracts of pipayeop, tinea skin extract, woobangja extract, eugeunpi extract, and galgeun extract were used.

In particular, the bipayeop, tinea skin, woobangja, Eugeunpi, and ginseng complex extract of the present invention has a synergistic or cooperative action between the extracts and/or active ingredients in the extract, compared to the individual extracts of each plant against acne causative bacteria. It was confirmed that there was a stronger antibacterial activity.

The complex extract contains a bivalve leaf extract, a white serrata extract, a chestnut extract, a Eugeunpi extract, and a ginseng root extract in a weight ratio of 1: 0.5-2.0: 0.1-1.5: 0.05-1.0: 0.05-1.0, preferably 1: 0.5-1.5: 0.1 -1.0:0.1-0.5:0.1-0.5 weight ratio, more preferably 1:0.7-1.3:0.3-0.7:0.1-0.4:0.1-0.4 weight ratio, more preferably 1:0.8-1.2:0.4-0.6 : 0.2-0.3: It may be included in a weight ratio of 0.2-0.3. When the weight ratio of each extract is within the above range, it is possible to maximize the synergistic effect of the combined use of the bivalve leaf extract, white sagebrush extract, woobangja extract, eugeunpi extract and ginseng root extract.

The bipayeop complex extract may be a fraction obtained by refractionation of a complex extract obtained by extracting bipayeop, baekseonpi, woobangja, eugeunpi, and brown root with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof with a second organic solvent. Preferably, the non-leaf leaf complex extract may be a fraction obtained by refractionation of the non-leaf leaf complex extract with hexane, ethyl acetate, butanol or water.

As used herein, the term "composite extract" used while referring to loquat leaves includes not only the crude extract obtained by treating the extract with an extraction solvent, but also the processed product of the loquat foliar complex extract. For example, the non-leaf complex extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

Also, in a broad sense, the non-leaf complex extract of the present invention is meant to include a processed product of the non-leaf leaf complex extract formulated so as to be able to administer to animals, for example, the non-leaf leaf complex extract powder. . Although the present invention conducted the experiment with the non-leaf leaf complex extract, it will be expected by those skilled in the art that the desired effect can be achieved even in the same form as the non-pariet complex extract.

In the present invention, the meaning of "contained as an active ingredient" means to include an amount sufficient to achieve the efficacy or activity of the extracts of bipayeop, baekseonpi, woobangja, yugunpi and galgeun extract.

In an exemplary embodiment, the bivalve leaf, tinea dermatophyte, champignon serrata, Eugeun derma and ginseng root complex extract may be present in the composition in any amount desired to provide effective antioxidant, skin moisturizing, skin acne improvement and/or skin trouble improvement effects. there is. According to one aspect, the extract of bibayeop, tinea skin, sagebrush, Eugeunpi, and black root complex extract may be 0.0001 to 99.9% by weight, preferably 0.001 to 30% by weight, more preferably 0.001 to 10% by weight, more preferably may be included in an amount of 0.01 to 10% by weight, more preferably 0.1 to 5% by weight. When the content of the bipayeop, tinea skin, woobangja, yugunpi and galgeun complex extract is less than the above range, it is difficult to expect the effect of the complex extract, and when it exceeds the above range, the increase in the effect according to the content increase is insignificant. In addition, there is a problem that the stability of the formulation is reduced. That is, by including the mixture of the present invention in the above range in the composition, excellent antioxidant, skin moisturizing, skin acne improvement and/or skin trouble improvement effects can be realized, formulation and product stability, and excellent effect with an optimal content in terms of economy can exert

The cosmetic composition of the present invention may further contain 10 to 30 parts by weight, preferably 15 to 25 parts by weight, of the lavender extract based on 100 parts by weight of the bipale leaf complex extract. When the lavender extract is included in the above weight ratio with respect to the extract of the bivalve leaf complex, the skin moisturizing effect, skin acne improvement and/or skin trouble improvement effects are further improved. When the content of the lavender extract with respect to the bivalve leaf complex extract is less than the lower limit, the effect due to the addition of the lavender extract becomes insignificant, and when the content of the lavender extract exceeds the upper limit, the increase in skin moisturizing, skin acne improvement and / Alternatively, there is a problem in that the skin trouble improvement effect is rather lowered.

The lavender extract is not particularly limited, but may be prepared in the same manner as the bipayeop extract.

According to a preferred aspect of the present invention, the formulation of the cosmetic composition for antioxidant, skin moisturizing, skin acne improvement or skin trouble improvement is a lotion, emulsion, cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, It may be one selected from the group consisting of a makeup base, foundation, powder, and makeup remover.

According to a preferred aspect of the present invention, the cosmetic composition for antioxidation, skin moisturizing, skin acne improvement or skin trouble improvement includes a dermatologically acceptable excipient in addition to the bipayeop, tinea skin, woobangja, eugeunpi and galgeun complex extract. may include

The excipient is not limited thereto, but may include, for example, an emollient, a skin penetration enhancer, a colorant, a fragrance, an emulsifier, a thickening agent, and a solvent. In addition, fragrances, dyes, bactericides, antioxidants, preservatives and moisturizing agents may be additionally included, and for the purpose of improving physical properties, thickeners, inorganic salts, synthetic polymer materials, and the like may be included.

According to a preferred embodiment of the present invention, the moisturizing agent is glycerin, butylene glycol, propylene glycol, sorbitol, hexylene glycol, dipropylene glycol, diglycerin, 1,2-hexanediol, panthenol, betaine, squalane, petrolatum, fluid It may be at least one selected from paraffin and hydrolyzed wheat gluten, but is not limited thereto.

The cosmetic composition of the present invention may be used alone or in overlapping application, or may be used in overlapping application with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

According to another aspect of the present invention, the present invention relates to a composition for external application for anti-oxidation, skin moisturizing, improving skin acne, or improving skin troubles, comprising a complex extract of bipayeop, tinea skin, woobangja, eugeunpi, and galgeun as active ingredients will be. In the present invention, the terms "composite extract of bivalve leaves", "skin acne", and "skin trouble" are the same as described above.

According to an embodiment of the present invention, the external preparation may be in the form of an ointment, patch, gel, cream or spray.

In the case of using the composition for external application for skin containing the complex extract of bipalea, tinea dermatophyllum, chrysanthemum rhizome, and ginseng root of the present invention as an active ingredient, additional fatty substances, organic solvents, solubilizers, thickening agents and gelling agents, emollients, Antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential It may contain adjuvants conventionally used in the field of dermatology, such as oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in external preparations for the skin. In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

According to another aspect of the present invention, the present invention provides a health functional food composition for antioxidant, skin moisturizing, skin acne improvement, or skin trouble improvement, comprising a complex extract of bipayop, tinea skin, woobangja, eugeunpi and galgeun as active ingredients is about In the present invention, the terms "composite extract of bivalve leaves", "skin acne", and "skin trouble" are the same as described above.

The health functional food composition of the present invention can be usefully used for preventing or improving skin acne or skin troubles.

The term "prevention" of the present invention means anything that suppresses or delays the above-mentioned skin acne or skin troubles.

As used herein, the term “improvement” refers to at least reducing parameters related to the condition to be treated, for example, the severity of symptoms, by administration of a composition comprising the extract of the present invention comprising the extract do.

The health functional food composition may be provided in the form of powder, granule, tablet, capsule, syrup or beverage, and the health functional food composition is used together with other foods or food additives in addition to the active ingredient, and is suitable according to a conventional method. can be used The mixed amount of the active ingredient may be suitably determined according to the purpose of its use, for example, prevention, improvement or therapeutic treatment. According to one aspect, the extract of bipayeop, tinea skin, Ubangja, Yugeunpi, and black root complex extract is 0.001 to 30% by weight, preferably 0.001 to 10% by weight, more preferably 0.01 to 10% by weight, more preferably 0.1 to It may be included in 5% by weight.

The effective dose of the active ingredient contained in the health food composition is the type and content of the active ingredient and other ingredients contained in the composition, the type of formulation, the age, weight, general health status, sex and diet, administration time, administration It may be adjusted according to various factors including the route, duration of intake, and drugs used at the same time, but is not limited thereto. In addition, in the case of long-term intake for the purpose of improving skin acne or skin troubles, it may be less than the above range, and since the active ingredient does not have any problem in terms of safety, it is certain that it can be used in an amount above the above range .

According to another aspect of the present invention, the present invention provides a skin acne or skin trouble prevention or pharmaceutical composition comprising the extract of bipayeop, tinea skin, woobangja, eugeunpi and galgeun complex extract as an active ingredient. In the present invention, the terms "composite extract of bivalve leaves", "skin acne", and "skin trouble" are the same as described above.

Through the pharmaceutical composition of the present invention, it is possible to provide the non-pariety complex extract at a pharmaceutically effective concentration to achieve the object and desired effect of the present invention. /kg, preferably 0.01 to 10 mg/kg, more preferably 0.1 to 5 mg/kg of dosage (or application amount) may be divided once or several times a day (administration or application).

The pharmaceutical composition includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers are those commonly used in formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginic acid, gelatin, calcium silicate, microcrystalline cellulose, poly vinylpyrrolidone, cellulose, purified water, syrup, methyl cellulose, methylhydroxybenzoic acid, propylhydroxybenzoic acid, talc, magnesium stearate, mineral oil, and the like. The pharmaceutical composition of the present invention may further include commonly used additives such as lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, and preservatives in addition to the above components.

The composition according to the present invention may be prepared in conventional pharmaceutical preparations, for example, tablets, granules, capsules, powders, liquid preparations, or syrups; Alternatively, it may be formulated as an ointment, warning agent, pasta agent, cataplasmase, cream, or emulsion, etc.

In addition, the composition according to the present invention may further comprise a pharmaceutically acceptable carrier. Carriers that can be used in the composition of the present invention are conventional in the pharmaceutical field, and for example, in the case of formulations for oral administration, there are binders, lubricants, disintegrants, excipients, solubilizers, stabilizers, etc., and for topical administration In the case of formulations, there are bases, excipients, lubricants, preservatives, and the like.

A suitable dosage of the pharmaceutical composition of the present invention may be prescribed variously depending on factors such as formulation method, administration mode, age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and response sensitivity of the patient. can

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited by the following examples.

< Examples and Comparative Examples>

Example 1: Preparation of the extract of the bivalve leaf complex

(1) Preparation of loquat leaf extract

After mixing the pulverized material of unleaved leaves and a 70% aqueous ethanol solution in a weight ratio of 1:20, extraction was performed at 80° C. for 3 hours to obtain an ethanol extract of unfoliated leaves. The obtained extract was filtered and concentrated under reduced pressure, and then powdered by freeze-drying.

(2) Preparation of Ringworm Skin Extract

The pulverized tinea skin powder and 70% ethanol aqueous solution were mixed in a weight ratio of 1:20 and extracted at 80° C. for 3 hours to obtain an ethanol extract of tinea skin. The obtained extract was filtered and concentrated under reduced pressure, and then powdered by freeze-drying.

(3) Preparation of extract of fenugreek

After mixing the pulverized material of fenugreek and 70% aqueous ethanol solution in a weight ratio of 1:20, extraction was performed at 80° C. for 3 hours to obtain an ethanol extract of fenugreek. The obtained extract was filtered and concentrated under reduced pressure, and then powdered by freeze-drying.

(4) Preparation of Yugeun skin extract

After mixing the pulverized lotus root and 70% ethanol aqueous solution in a weight ratio of 1:20, extraction was performed at 80° C. for 3 hours to obtain an ethanol extract of Yugeunpi. The obtained extract was filtered and concentrated under reduced pressure, and then powdered by freeze-drying.

(5) Preparation of Root Extract

The ground root ground root and 70% ethanol aqueous solution were mixed in a weight ratio of 1:20 and extracted at 80 °C for 3 hours to obtain an ethanol extract of ground root root. The obtained extract was filtered and concentrated under reduced pressure, and then powdered by freeze-drying.

(6) Preparation of the extract of loquat foliar complex

The bivalve leaf extract, baekseonpi extract, woobangja extract, eugeunpi extract, and black root extract were mixed in a weight ratio of 1:1:0.5:0.25:0.25 to prepare a bipale leaf complex extract.

Example 2: Preparation of the extract of the bivalve leaf complex

In the same manner as in Example 1, except that, in step (6), the bivalve leaf extract, chrysanthemum extract, chestnut extract, Eugeunpi extract, and black root extract were mixed in a weight ratio of 1: 1: 1: 1: 1 to prepare a bivalve leaf complex extract. .

Example 3: Preparation of loquat leaf complex extract - addition of lavender extract

After mixing the pulverized lavender with a 70% aqueous ethanol solution in a weight ratio of 1:20, extraction was performed at 80° C. for 3 hours to obtain an ethanol extract from the leaves. The obtained extract was filtered and concentrated under reduced pressure, and then powdered by freeze-drying.

By mixing 20 parts by weight of the lavender extract with respect to 100 parts by weight of the non-leaf leaf complex extract prepared in Example 1, a non-leaf leaf complex extract was prepared.

Comparative Example 1: Bipayeop extract alone

An extract of bivalve leaves was prepared in the same manner as in step (1) of Example 1.

Comparative Example 2: Ringworm extract alone

A tinea skin extract was prepared in the same manner as in step (2) of Example 1.

Comparative Example 3: Algae extract alone

An extract of U.S. basilica was prepared in the same manner as in step (3) of Example 1.

Comparative Example 4: Eugeunpi extract alone

A Eugeunpi extract was prepared in the same manner as in step (4) of Example 1.

Comparative Example 5: Garlic root extract alone

The extract of the ginseng root was prepared in the same manner as in step (5) of Example 1.

< Test Example>

statistical processing

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). The difference (p <0.05) between the experimental groups was determined using one-way ANOVA using Duncan's post-hoc test. Each of the following experiments was repeated at least 3 times, and each experimental data was expressed as the mean ㅁ standard deviation (SD).

Test Example 1: Cytotoxicity evaluation using MTT assay

In order to examine the effect of the MTS assay method on the cell viability of the sample obtained in the above example, an experiment was performed as follows using the method described in the existing literature (Choi JH et al ., Flowers of Inula japonica attenuate inflammatory responses, Immune Network 2010, 10(5), 145-152).

First, Raw 264.7 cells were aliquoted at a density of 5×10 ⁴ cell/mL in a 24-well plate and cultured at 5% CO ₂ , 37° C. conditions. After 24 hours, the samples according to Examples 1 to 3 were treated with each concentration (0.1, 0.5, 1.0, 5.0 mg/ml) and further cultured for 24 hours. Then, the supernatant was removed, 1 μg/mL of MTT solution was added, and left for 2 hours. Then, 100 μl of DMSO was added per well to dissolve formazan crystal for 20 minutes, and absorbance was measured at 540 nm with an ELISA plate reader. Cell viability was calculated by Equation 1 below, and the results are shown in Table 1 below as relative ratios to the control group.

[Equation 1]

Cell viability (%) = (absorbance of sample treatment group / absorbance of control group) × 100

sample (mg/mL) Cell viability (%) Control group (untreated group) 100 Example 1 0.1 113.1 0.5 108.6 1.0 98.7 10.0 95.5 Example 2 0.1 105.7 0.5 102.1 1.0 99.4 10.0 92.6 Example 3 0.1 115.2 0.5 109.8 1.0 103.7 10.0 96.1

Referring to Table 1, it can be seen that the complex extract according to an embodiment of the present invention does not exhibit significant toxicity up to a concentration of 10.0 mg/mL.

Test Example 2: Antioxidant activity (DPPH radical scavenging ability)

DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging ability was measured using the method of Cho et al. (2011). The non-leaf leaf complex extract according to the example was diluted in DMSO and adjusted to concentrations of 0.1, 0.5, and 1.0 mg/ml, respectively. Then, 0.1 mL of a DPPH (Sigma-Aldrich Co, USA.) solution having a concentration of 0.4 mM was added to 0.1 mL of each concentration sample, followed by reaction at room temperature for 30 minutes. Absorbance at 515 nm was measured using a microplate reader (Thermo Fisher Scientific, Rockford, IL, USA), and after calculation as in Equation 2 below, the results are shown in Table 2 below. As a positive control, ascorbic acid (50 μg/μl), a representative antioxidant, was used.

[Equation 2]

DPPH radical scavenging ability (%) = [(Ac-As) / Ac] × 100

(Ac: control absorbance, As: sample group absorbance)

sample (mg/mL) DPPH scavenging ability (%) Control group (untreated group) - positive control 100 Example 1 0.1 48.2±3.6 0.5 59.8±4.0 1.0 76.5±2.4 Example 2 0.1 43.1±1.2 0.5 54.7±2.9 1.0 71.8±2.3 Example 3 0.1 56.1±1.5 0.5 68.2±3.1 1.0 83.3±0.7 Comparative Example 1 0.1 34.0±0.6 0.5 46.9±3.5 1.0 60.6±2.4

As shown in Table 2, it can be confirmed that the bipayeop complex extract according to the example exhibits a stronger antioxidant effect than the bipayeop extract of Comparative Example 1.

Test Example 3: Nitric oxide (NO) production inhibitory effect

In order to confirm the skin trouble improvement effect and anti-inflammatory effect of the bivalve leaf complex extract, a nitric oxide (NO) production inhibitory test was conducted by the GRIESS method using the RAW264.7 cell line (ATCC number: CRL-2278).

It was dispensed to a 96-well plate at 2×10 ⁵ cells/well per well, and then incubated at 37° C., 5% CO ₂ in an incubator for 24 hours. After overnight, it was exchanged with a fresh medium, and LPS (Lipopolysaccharide) (1 μg/mL) as a stimulus and the samples of Examples and Comparative Examples were administered at concentrations of 0.1, 0.5 and 1.0 mg/mL, and incubated for 24 hours. After incubation, the medium supernatant was taken and centrifuged at 13,000 rpm for 3 minutes to collect only the supernatant, reacted with Griess reagent 1:1, and absorbance was measured at 570 nm, and the result was a negative control (LPS alone treatment) The relative ratios are shown in Table 3 below. As a positive control, an NO-production inhibitor, L-NMMA (L-NG-Monomethylarginine), was used at a concentration of 10 μg/mL.

[Equation 3]

NO production inhibition rate (%)={(absorbance of negative control group - absorbance of each extract)/absorbance of negative control} × 100

division NO production inhibition rate (%) 0.1 mg/mL 0.5 mg/mL 1.0 mg/mL negative control - L-NMMA 40.23 Example 1 14.5±1.1 27.3±0.7 34.6±2.0 Example 2 12.2±2.4 24.8±3.5 31.3±3.3 Example 3 20.5±4.7 33.6±3.4 38.5±1.6 Comparative Example 1 6.3±3.2 14.2±5.3 22.2±7.8 Comparative Example 2 4.2±2.4 9.5±4.2 13.9±2.5 Comparative Example 3 4.1±1.3 11.4±6.3 16.3±1.1 Comparative Example 4 3.4±2.1 10.1±4.7 15.7±2.0 Comparative Example 5 4.6±0.5 6.9±1.2 10.6±1.6

As shown in Table 3, it can be confirmed that the non-pariety complex extract of Examples has a significant NO production inhibitory effect compared to the single extracts of Comparative Examples 1 to 5.

In particular, it was confirmed that when the concentration of the bipayeop complex extract of Example is 1.0 mg/mL or more, it exhibits excellent NO production inhibitory efficacy to a degree similar to that of L-NMMA, which is known as an NO production inhibitor.

Test Example 4: Antibacterial activity against acne causative bacteria

In order to check whether the extracts according to Examples and Comparative Examples have direct antibacterial activity against the acne-causing bacteria, Propionibacterium acnes known as the acne-causing bacteria, the Staphylococcus epidermidis strain, and The effect of inhibiting the growth of acne-causing bacteria was verified for Staphylococcus aureus strains.

strain name Strain No. culture conditions P. acnes KCTC 5012 Anaerobic, 37°C, 48h S. epidermidis KACC 13234 Aerobic, 30℃, 48h S. aureus KCTC 3881 Aerobic, 30℃, 24h

For verification, the minimum growth inhibitory concentration (Minimun Inhibitory Concentration, MIC) for Propionibacterium acnes strain, Staphylococcus epidermidis strain, and Staphylococcus aureus strain was obtained according to the broth dilution method.

First, the Propionibacterium acnes strain was cultured using RCM medium, Staphylococcus epidermidis strain was NB medium, and Staphylococcus aureus strain was NA medium. After culturing under the culture conditions of Table 4 used in the test.

Experimental groups according to Examples and Comparative Examples were prepared by mixing a liquid medium for each strain, and a sample according to Examples or Comparative Examples, respectively, and the three strains were prepared with an initial number of 1 × 10 ⁶ CFU/ml, respectively. Afterwards, a microbial reduction efficacy test was performed. After inoculating each of the three prepared strains into the experimental groups according to Examples and Comparative Examples, they were cultured under optimal conditions for each strain, and samples were collected every 0, 1, and 24 hours, and then diluted 10-fold with sterile saline to agar medium. smeared on

After culturing the plated plates under optimal conditions, the reduction rate was confirmed by confirming and counting typical colonies, thereby confirming the reduction efficacy against three types of microorganisms. For comparative verification, 0.1% (w/v) Salicylic acid (positive control 1) and 1% (w/w) Tea Tree oil (positive control 2), which are antibacterial substances well known for their antibacterial effect against conventional acne causative bacteria, were used. .

division MIC (ppm) P. acnes S. epidermidis S. aureus negative control - - - Positive Control 1 1000 780 2500 positive control 2 3000 2000 >10000 Example 1 310 250 400 Example 2 390 325 450 Example 3 200 170 250 Comparative Example 1 1400 1000 1500 Comparative Example 2 7500 >10000 8000 Comparative Example 3 >10000 >10000 >10000 Comparative Example 4 2500 1500 6000 Comparative Example 5 5000 7500 >10000

As shown in Table 5, it was confirmed that the experimental group treated with the bivalve leaf complex extract according to the example had significantly higher antibacterial activity against the acne causative bacteria than the experimental group treated with the single extract of Comparative Examples 1 to 5.

In particular, it was measured that the antibacterial effect of the non-pale leaf complex extract of Example 3 was the best, and even more excellent effects were observed compared to the positive control group. From the above results, it can be seen that the bivalve leaf complex extract of the present invention can be very usefully used to improve acne-prone skin by effectively killing acne-causing bacteria.

<Production Example 1> Preparation of cosmetics (essence)

Essence was prepared as a cosmetic composition to which the extracts according to Examples and Comparative Examples were respectively applied with the composition shown in Table 6 below.

Ingredients content (wt%) Preparation 1-1 Preparation 1-2 Preparation 1-3 Comparative Preparation Example 1 Comparative Preparation Example 2 Comparative Preparation Example 3 Comparative Preparation Example 4 Comparative Preparation Example 5 Example 1 3.0 Example 2 3.0 Example 3 3.0 Comparative Example 1 3.0 Comparative Example 2 3.0 Comparative Example 3 3.0 Comparative Example 4 3.0 Comparative Example 5 3.0 triethanolamine 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Carboxyvinyl Polymer (1) 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 propylene glycol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 paraoxybenzoic acid
ester 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 beeswax 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 cetostearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 glyceryl mono
stearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 monostearic acid
Sorbitan(2) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 monostearic acid
Polyethylene glycol 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 propyl paraoxybenzoate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Dimethylsiloxane 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 isocetyl
Octanoate (3) 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Spices appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount Distilled water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount

* Note ¹ : Carboxyvinyl polymer - 1 wt% aqueous solution of Carbopol 941 manufactured by BFGoodrich, USA

* Note ² : Sorbitan monostearate - Arlacel 60 from ICI, UK

* Note ³ : Isocetyl octanoate - ICEH from Nihon KoKyu Alcohol, Japan

Test Example 5: Skin moisturizing effect

The clinical moisturizing effect and lasting power of the cosmetic compositions of Preparation Examples and Comparative Preparation Examples were measured as follows.

After selecting 80 healthy adult males and females who feel dry skin through a survey, randomly dividing them into 8 groups (10 people each), in each group, Preparation Examples 1-1 to 1-3 and Comparative Preparation Examples 1 to The essence prepared according to 5 was applied to the entire face twice daily (morning/evening) for 30 days. At this time, before applying the essence, measure the moisture content of the skin using a moisture meter (Corneometer, CM825 courage Khazaka electronic GmbHtk, Germany) under constant temperature and humidity conditions (24 ℃, 40% humidity) and set this as a default value, 30 After the elapse of days, the amount of skin moisture was measured, and the amount of change thereof was evaluated. For reference, the measurement value of the moisture meter increases as the amount of skin moisture increases, and the measurement coefficient is an Arbitrary unit (A.U.). The experimental results are shown in Table 7 below.

division before application after 30 days Skin moisture increase rate (%) Control group (untreated group) 25.76 26.03 1.05 Preparation 1-1 24.95 46.27 85.45 Preparation 1-2 24.20 43.15 78.31 Preparation 1-3 24.28 50.31 107.21 Comparative Preparation Example 1 23.90 38.18 59.75 Comparative Preparation Example 2 23.50 31.09 32.30 Comparative Preparation Example 3 23.22 25.64 10.42 Comparative Preparation Example 4 25.23 34.46 36.58 Comparative Preparation Example 5 23.19 29.77 28.37

As shown in Table 7, it was confirmed that the cosmetic composition according to Preparation Example had significantly higher skin moisturizing effect and durability than the cosmetic composition of Comparative Preparation Example.

Test Example 6: Human patch test

The human patch test was conducted in accordance with the Code of Ethics based on the Declaration of Helsinki and the guidelines for human application of cosmetics by the Ministry of Food and Drug Safety, and the method devised by Frosch & Kligman (J. Am. Acad. Dermatol, 1(1) 35 (1979)) ) was applied to 32 healthy female subjects aged 20 to 60 years (mean age 41.34ㅁ8.06) without specific skin diseases or allergies. First, the patch site was disinfected with 70% ethanol, and then, Van der Bend (Van der Bend, Netherlands) to which 20 μl of the samples of Examples 1 to 3 (5.0 mg/mL) was applied was patched. After 48 h, the patch was removed, the test site was marked with a skin marker (Chemotechnique Diagnostics AB, Sweden), and the reactivity of each test site was evaluated after 20 min and 24 h. The evaluation criteria of the primary skin irritation test (Skin Primary irritation Test) were based on the judgment criteria of the PCPC Guidelines presented in Table 8 below, and the degree of skin reaction (average reactivity) evaluated twice according to the following Equation 4 was scored.

reactivity Clinical symptoms (response) 0 no response One faint or mild erythema 2 moderate erythema; and barely detectable edema and/or papule formation at the patch border 3 moderate erythema; and edema that has spread throughout the patch site 4 severe erythema; and severe edema; or blistering 5 Severe reaction spread beyond the patch site

[Equation 4]

Average reactivity = [{(reactivity × number of subjects who responded)/(total number of subjects × highest score (5 points))} × 100] × 1/2

In order to evaluate the degree of skin irritation on human skin, 20 μL of the liquid samples of Examples 1 to 4 were applied to 32 subjects, respectively, and a human patch test was performed, and the results are shown in Table 9 below.

division Number of subjects who responded (persons) reactivity 24 hours 48 hours Average negative control 0 0.0 0.0 0.0 Example 1 0 0.0 0.0 0.0 Example 2 0 0.0 0.0 0.0 Example 3 0 0.0 0.0 0.0

Looking at Table 9, the composition according to the embodiment of the present invention was applied to evaluate the degree of skin irritation, and as a result of evaluating the degree of skin irritation, all 32 subjects showed a negative reaction with a response grade of 0.0, so the composition of the present invention was the primary stimulation of human skin It is judged to be a non-irritating material from the side.

Examples of preparation for the non-leaf leaf complex extract of the present invention are exemplified below.

<Production Example 2> Preparation of cosmetics

<2-1> Softening lotion (skin)

In order to prepare a softening lotion for antioxidant, skin moisturizing, skin acne improvement or skin trouble improvement containing the bipale leaf complex extract of the present invention, it is formulated according to a conventional manufacturing method in the cosmetic field by mixing as described in Table 10 below. can do.

ingredient content (wt%) Example 3 non-leaf complex extract 2.0 glycerin 6.0 1,3-butylene glycol 3.0 PG 1500 1.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

<2-2> Nutrient lotion (lotion)

In order to prepare a nutrient lotion for antioxidant, skin moisturizing, skin acne improvement or skin trouble improvement containing the bipale leaf complex extract of the present invention, it is formulated according to a conventional manufacturing method in the cosmetic field by mixing as described in Table 11 below. can do.

ingredient content (wt%) Example 3 non-leaf complex extract 2.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 tocopheryl acetate 3.0 liquid paraffin 5.0 squalane 3.0 Macadamin Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

<2-3> Nourishing Cream

In order to prepare a nourishing cream for antioxidant, skin moisturizing, skin acne improvement or skin trouble improvement containing the bipale leaf complex extract of the present invention, it is formulated according to a conventional manufacturing method in the cosmetic field by mixing as described in Table 12 below. can do.

ingredient content (wt%) Example 3 non-leaf complex extract 2.0 lipophilic monostearate glycerin 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearylisostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

<2-4> Latex for mask pack

In order to prepare an emulsion for antioxidation, skin moisturizing, skin acne improvement or skin trouble improvement mask pack containing the bivalve leaf complex extract of the present invention, it is formulated as described in Table 13 below and followed according to a conventional manufacturing method in the cosmetic field. can be manufactured.

ingredient content (wt%) Example 3 non-leaf complex extract 2.0 polyvinyl alcohol 15.0 Cellulose Gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-Panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

<Production Example 3> Preparation of health functional food

<3-1> Manufacture of health drinks

A health drink containing the bipayeop complex extract of the present invention with the ingredients and contents shown in Table 14 below was prepared according to a conventional method.

After mixing the ingredients in Table 14 according to the usual health drink manufacturing method, after stirring and heating at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demanding country, and use.

ingredient content Example 3 non-leaf complex extract 500 mg citric acid 1000 mg oligosaccharide 10 g Plum Concentrate 2.0 g Taurine 1.0 g Purified water remaining amount Sum 100g

Although the present invention has been described as the above-mentioned preferred embodiment, it is possible to make various modifications and variations without departing from the spirit and scope of the invention. It is also intended that the appended claims cover such modifications and variations as fall within the scope of the present invention.

Claims (7)

A cosmetic composition for antioxidation, skin moisturizing, skin acne improvement or skin trouble improvement, comprising a complex extract of bipayeop, tinea skin, woobangja, eugeunpi, and ginseng root as active ingredients. According to claim 1,
The composition is a cosmetic composition, characterized in that it has nitric oxide (NO) production inhibitory activity. According to claim 1,
The composition is Propionibacterium acnes strain, Staphylococcus epidermidis strain, and Staphylococcus aureus ) Cosmetics, characterized in that it has antibacterial activity against the strain composition. According to claim 1,
The complex extract is a cosmetic composition, characterized in that it contains a bivalve leaf extract, a baekseonpi extract, a chestnut extract, a Eugeunpi extract, and a ginseng root extract in a weight ratio of 1: 0.5-2.0: 0.1-1.5: 0.05-1.0: 0.05-1.0. According to claim 1,
The complex extract is a cosmetic composition, characterized in that 0.001 to 10% by weight based on the total weight of the composition. According to claim 1,
The composition is a cosmetic composition, characterized in that it further comprises 10 to 30 parts by weight of lavender extract based on 100 parts by weight of the complex extract. A health functional food composition for antioxidant, skin moisturizing, skin acne improvement, or skin trouble improvement, comprising a complex extract of bipayop, tinea skin, woobangja, eugeunpi, and ginseng root as active ingredients.