KR20100010316A - Cosmetic composition for preventing skin aging - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging containing a herbal extract mixture, more specifically, herbal extract consisting of white paper, Dongguin, Cheongung, Dongle, Sessin, Kwakeuneun, Doin, Bark skin, Sinyi, saengsonghyang and Baeksijam Herbal extract mixture (hereinafter referred to as 'Gagamcheon Forbidden') and herbal extract mixture consisting of ginseng, sulfur and honey (hereinafter referred to as 'Gagamkyung Okgo') and herbal extract mixture consisting of passerby, fruit root and Baekbongryeong (hereinafter referred to as 'Gagam Hongoksan') It relates to a cosmetic composition for preventing skin aging containing as an active ingredient. The cosmetic composition of the present invention is safe for the skin, and prevents and improves skin aging by improving cell proliferation and metabolic activity, free radical scavenging, improving skin wrinkles and improving skin elasticity by promoting collagen production.

Description

Cosmetic composition for preventing skin aging {Cosmetic composition for preventing skin aging}

The present invention relates to a cosmetic composition for preventing skin aging containing a herbal extract mixture, more specifically, herbal extract consisting of white paper, Dongguin, Cheongung, Dongle, Sessin, Kwakeuneun, Doin, Bark skin, Sinyi, saengsonghyang and Baeksijam Herbal extract mixture (hereinafter referred to as 'Gagamcheon Forbidden') and herbal extract mixture consisting of ginseng, sulfur and honey (hereinafter referred to as 'Gagamkyung Okgo') and herbal extract mixture consisting of passerby, fruit root and Baekbongryeong (hereinafter referred to as 'Gagam Hongoksan') It relates to a cosmetic composition for preventing skin aging containing as an active ingredient.

The skin of the human body is a part directly exposed to the external environment, and when exposed to excessive ultraviolet rays or pollutants, free radicals or free radicals are generated, and erythema, edema, rashes, tingling, and itching by the generated free radicals or free radicals Skin irritation and inflammatory reactions are induced. Skin troubles caused by these causes are not only aesthetic problems, but also substances produced during the inflammatory process may cause pigmentation of the skin and accelerate the breakdown of skin elastic fibers, thereby affecting the increase of skin wrinkles. It is known to lead to skin aging. Therefore, one of the areas of interest in cosmetics is how to delay skin aging. The final phenomena that appear in accordance with aging of the skin include wrinkle formation, skin elasticity reduction, aging spot formation, and the like, in particular, wrinkle formation is accepted as the most representative phenomenon.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111: 31-37 (1996)). Endogenous aging is a aging phenomenon that occurs naturally as the skin ages and the physiological function of the skin gradually decreases.

It is clinically characterized by reduced elasticity, rough skin texture, deep wrinkles and pigmentation (Arch Dermatol., 130: 87-95 (1994)). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress. And by these factors rapidly progressing aging of the skin, resulting in wrinkles, rough skin surface is generated is significantly reduced elasticity. (Physiology Of The Skin , Allured Publishing Corporation, Peter T. Pugliese.MD, p61 -72 )

Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, when the skin is exposed to harmful free radicals or free radicals, lipid peroxide is produced by oxidative action in the body and causes modification of various proteins constituting the skin. On the other hand, collagen and elastin, which are the major structural components of the skin that make up most of the dermis (about 70-80% of the total dry weight of the skin), are denatured or reduced in biosynthesis by natural or external stimuli, which accelerates skin aging. do.

In detail, the skin is divided into epidermis and dermis according to the structure of the skin. In the epidermis, the turnover of keratinocytes is reduced, the accumulation of the stratum corneum and the thickening of the stratum corneum and the water-containing ability This decreases and the keratin cures and wrinkles are generated.

In addition, the epidermal and dermal borders are involved in epidermal cell support, adhesion, transport of nutrients, and regulation of epidermal differentiation. As a result, the selective permeation function is weakened so that harmful components easily affect the dermis and wrinkles are generated. (Keene, J. Cell Biol., 104, p611 -621, 1987)

In addition, the dermis is a tissue composed of connective tissue below the epidermis, which is not densely packed like the epidermal keratinocytes but has a lot of extracellular space and is filled by a network of macromolecules called extracellular matrix. . This extracellular epilepsy is made from fibroblasts in the dermis and consists of fibrous proteins such as collagen and elastin and polysaccharides called acidic mucopolysaccharides such as hyaluronic acid. Extracellular epilepsy is directly involved in skin elasticity, metabolism and vitality. In more detail, the acidic mucopolysaccharide retains a large amount of water in its molecule to form a gel, and water in the gel is responsible for penetrating nutrients, metabolites, and hormones from blood vessels to cells in tissues. . Therefore, when the production of acidic mucopolysaccharides in extracellular epilepsy is promoted, not only the skin is fresh but also the metabolism of the skin is promoted, and skin aging can be prevented. In addition, the fibrous protein is responsible for maintaining the shape of the tissue and imparting elasticity to the skin. Therefore, when the production of fibrous protein is promoted in extracellular epilepsy, elasticity is given to the skin and wrinkles or fine lines are suppressed. When the activity of fibroblasts producing such extracellular epilepsy is degraded by internal and external aging factors, particularly UV, collagen biosynthesis decreases and denatured elastin increases. Collagen and elastic elastin proteins are the major proteins produced by fibroblasts and are involved in the skin's mechanical firmness, tissue adhesion and elasticity in the dermal layer of the skin (Journal of the american academy of dermatology, 21: 610-613 (1989)). . Collagen decreases with age and with increasing exposure to ultraviolet light. Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen types I and III constitute the constituents of the cytoplasm of the dermis, and collagen type IV is the major component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermal and dermal layers of the skin to regulate the permeation and transport of materials between the two layers, to control the differentiation of adjacent skin cells and to maintain the firmness of the skin. Is an important part of the skin aging process.

In order to suppress the skin aging or to slow the progression, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherol have been developed. However, such cosmetics or skin protectors have a problem in that the potency is low in the final formulation, the efficacy is slight, and the skin irritation is severe.

Recently, due to the skin irritation problem of these raw materials, the development of cosmetics using natural vegetable raw materials that are safer and have no skin irritation have been made. In particular, in Korea, we are developing a lot of cosmetics using natural herbal ingredients. This is because natural herbal ingredients have been used in private for a long time and safety and effectiveness have already been secured. Because there is.

Therefore, the present inventors conducted research on natural natural products that have been used as a herbal medicine in the past, and as a result, white paper, dongwain, cheongung, dongle, sessin, earthen root, caucus, bark skin, shinyi, gamsonghyang, Baekgangjam It is excellent in improving skin wrinkle and skin elasticity by enhancing cell proliferation and metabolic activity, free radical scavenging, and promoting collagen production in the herbal mixture consisting of mixed extract and ginseng, turmeric, honey mixed extract and algae, fruit root, Baekbongryeong mixed extract Found.

In the prior patents, moisturizing and whitening in the case of white paper (Korean Patent Publication 2006-92179), for the removal of waste (Korean Patent Publication 2002-93655), for the removal of waste in the case of copper and wine (Korean Patent Publication 2002-93655) ), Moisturizing for Koreans (Korean Patent Publication No. 1997-32840), moisturizing for earthwork muscles (Korean Patent Publication 2002-93655), moisturizing and whitening (Korean Patent Publication 2006-92179), whitening (Korean Patent Publication No. 2000-20728, Korean Patent Publication No. 2003-21811), In case of fruiting roots, there are examples used for removing waste matter (Korean Patent Publication No. 2002-93655), beauty (1995-7824), but examples used for anti-aging There was no. In addition, the herbal ingredients of the present invention, white paper, ginkoin, cheongung, dongle, sessin, earthen root, doin, velvet skin, shinyi, saengsonghyang, baekgangjam and ginseng, jihwang, honey, almond, fruit root, Baekbongryeong There is no mention of a cosmetic composition for preventing skin aging.

Therefore, the inventors of the present invention is a mixed extract (gagamcheoncheon forbidden) consisting of white paper, dongguin, cheonggung, roundle, sessin, earthworm, doin, bark skin, shinyi, persimmon fragrance and baekgangjam and mixed extract consisting of ginseng, sulfur and honey And the proliferation of fibroblasts that biosynthesize extracellular epilepsy, which is the most important role in maintaining the skin without wrinkles as described above. In addition to smoothing metabolism, it has the effect of promoting biosynthesis and excellent free radical inhibiting ability, thereby fundamentally preventing skin aging caused by wrinkles, fine lines, rough skin, and the like, and found no problem in stability. In addition, the synergistic effect of the three mixed extracts were found to exert a more excellent effect in preventing and improving skin aging to complete the present invention.

An object of the present invention is to provide a skin anti-aging cosmetic composition comprising a herbal extract mixture consisting of white paper, gingin, cheongung, dongle, sessin, earthworm, cauliflower, bark skin, shinyi, saengyanghyang and baekjap as an active ingredient. .

An object of the present invention is to provide a cosmetic composition for preventing skin aging, comprising the herbal extract mixture consisting of ginseng, sulfur and honey.

It is an object of the present invention to provide a cosmetic composition for preventing skin aging, comprising a herbal extract mixture consisting of algae, fruit root and Baekbongnyeong as an active ingredient.

  An object of the present invention is a herb extract mixture consisting of white paper, Dongguin, Cheongung, roundle, sessin, earthworm, Doin, bark skin, Shinyi, saengsonghyang and Baekjajam and herbal extract mixture consisting of ginseng, turmeric and honey, and passersby And it is to provide a cosmetic composition for preventing skin aging comprising all of the herbal extract mixture consisting of Baekbongryeong as an active ingredient.

An object of the present invention is to provide a cosmetic composition for preventing skin aging, characterized in that it has a cell proliferation and metabolic activity effect.

An object of the present invention is to provide a cosmetic composition for preventing skin aging, characterized in that it has a free radical scavenging effect.

An object of the present invention is to provide a cosmetic composition for preventing skin aging, characterized in that it has a collagen production promoting effect.

An object of the present invention is a herb extract mixture consisting of white paper, Dongguin, Cheongung, roundle, sessin, earthworm, Doin, bark skin, Shinyi, saengsonghyang and Baekjajam and herbal extract mixture consisting of ginseng, turmeric and honey, and passersby And to provide a method for producing a skin anti-aging cosmetic composition comprising the herbal extract mixture consisting of Baekbongryeong as an active ingredient.

According to an aspect of the present invention, the present invention provides a skin anti-aging cosmetic composition comprising an herbal extract mixture consisting of white paper, gingiwine, celestial organ, roundle, sesins, earthworm muscle, cabbage, bark skin, shinyi, saengsonghyang and napja To provide a composition.

According to another aspect of the present invention, the present invention provides a cosmetic composition for preventing skin aging, comprising as an active ingredient a herbal extract mixture consisting of ginseng, sulfur and honey.

According to another aspect of the present invention, the present invention provides a cosmetic composition for preventing skin aging, comprising as an active ingredient a herbal extract mixture consisting of algae, fruit roots and Baekbongnyeong.

According to another aspect of the present invention, the present invention provides a cosmetic composition for preventing skin aging comprising all three herbal extract mixtures as an active ingredient.

That is, the present invention is a herbal medicine extract mixture consisting of white paper, dongguin, cheonggung, roundle, sessin, earthworm, doin, bark skin, sinyi, saengsonghyang and baekgangjam and herbal extract mixture consisting of ginseng, turmeric and honey, and passersby And it provides a cosmetic composition for preventing skin aging comprising all of the herbal extract mixture consisting of Baekbongryeong as an active ingredient.

The present invention shows a synergistic effect by applying all three herbal extracts mixed extract at the same time. The term "synergy" in the present invention refers to the synergy or cooperation of each function that contributes to the overall effect by producing more than the combined effect of each of the different herbal extract mixtures. As shown in Experimental Examples 4 to 7, according to the present invention, when three herbal extract mixtures were simultaneously applied compared to the respective herbal extract mixtures, the skin metabolism activity, cell proliferation, collagen synthesis and more than the combined effects of each skin Anti-aging, improvement and therapeutic effect.

Cnidium officinale Makino used in the present invention is a perennial herb, 40-70 cm in height, and has the characteristic of smelling plants. The root of the uterus is an irregular nodular lump, 2-7 cm in diameter, yellowish brown with coarse wrinkles, several parallel raised bumps, and a top of the concave circular stem trace. Its cross section is yellowish white or greyish yellow with scattered yellowish brown oil and the formation layer is wavy yellowish gate. The root of Cheongung is used for various gynecological diseases such as cold syndrome, anemia and dysmenorrhea disorder with the aim of blood, tonic, soothing and pain relief in oriental medicine. The uterus inhibits the activity of melanocyte stimulating hormones, which in turn inhibits the production of tyrosinase, inhibits melanin production, thereby preventing pigmentation and exerts a whitening effect. It is a perennial plant of the family Apiaceae, native to China and grown as a medicinal crop. Its height is 30-60 cm and its components are known as cnidilide, ligustilide, neocnidilide, butylphthalide and sedanonic acid. Cheonggung was used to dry the root stem in oriental medicine and used for gynecological diseases such as uterine sedation, blood pressure control, antibacterial action, inhibitory effect on dermatophytes, headache, anemia, infertility, dysmenorrhea and coldness. It was put in the closet for some prevention in the private sector (Information, Shin Min-kyo, Ambassador of Yakyang, pp418, 1998).

As used in the present invention (Magnolia denudata Desrousseaux) is a deciduous shrub cultivated in gardens in southern China, the wild is less. It belongs to Magnoliaceae, and it is picked up and dried in the sun before spring buds open. The plant of origin is Magnolia denudata. Besides, the Korean herbal medicine standard contains magnolia kobus, Magnolia salicifolia Maxim.The Chinese Pharmacopoeia defines Magnolia biondii, Magnolia sprengeri, Magnolia liliflora, Magnolia faragesii. Shin is traditionally known to relieve sore bones with wind, to get rid of freckles on the face, to heal noses and runny noses, to brighten the eyes, and to produce beards and hair (primary wood). In addition, research has been reported on cosmetic compositions (Japanese Patent Laid-Open Publication No. 5-320034), skin disease treatment agents (Japanese Patent Laid-Open Publication No. 5-246874), and deodorant (Japanese Patent Laid-Open Publication No. 5-212099) having a skin whitening effect and an anti-inflammatory effect in association with sinusitis. However, it is completely distinct from the present invention. Existing god-identification studies have reported substances such as Magnolol, Syringin, Honokiol, Eugenol, and Thyramine.

The white paper (Angelica Dahuricae Radix) used in the present invention originates from a copper clam, which is a umbelliferae plant, and is a perennial herb and grows in grassy wetlands or shrubs or rivers. Collect roots in autumn and dry them in the sun. Existing ingredient identification studies show that Kumarins, Byak-angelicin, Oscine, Xangthotoxin, (-)-Nokadekenetin, etc. The substance is known. White paper is traditionally known to cure head soreness due to heat or wind. Herbal medicine, pain relief, hemostatics, used as a sedative. In studies related to white paper, the treatment of dermatitis by promoting sebum secretion (Japanese Patent Laid-Open No. 9-176030), hair growth inhibitor with excellent human stability (Japanese Patent Laid-Open No. 10-139639), and bathing agents with increased warming effect (Japanese Patent Laid-Open No. 06-206814) It is known that the white paper is made of plasters and powders in Dongbobogam and Shinnong-bonbyeon, and when applied to the face, it sends warm energy to the facial skin to promote blood circulation, oiling the complexion, and removing blemishes, freckles, and scars on the face. Doing.

The persimmon scent (Nardostachys chinensis Batal) used in the present invention is a perennial herb and grows in alpine pastures or grassland trenches, and is collected in spring and autumn to use underground diameters or roots. Persimmon is a member of the family Valerianaceae. The plant of origin in Korea is Nardostachysis chinensis Batal. In addition to this, it is defined as Nardostachysis jatamansi in China. Persimmon scent has traditionally been used in various herbal medicines such as bloating pain, vomiting, loss of appetite, and toothache. In addition, researches on cosmetic compositions (see Japanese Patent Application Laid-Open No. 8-259423) and dermatological treatments (Japanese Patent Application Laid-open No. 5-246874) have been reported in connection with sweet odor. However, no reports of radical scavenging effects have been reported. Existing research on the sensitizing component has reported compounds such as ethyl-β-D-glucopyranoside, Narstastachin, Kanshone, and Nardofuran.

Ginseng (Panax ginseng CA Mey.) Used in the present invention is a perennial herb, a component such as Ginsenosides, Damanarane, Oleanolic acid, Panaxynol, and the like. In terms of skin beauty, it has effects of promoting metabolism, preventing skin aging, inhibiting abnormal cells, and promoting blood circulation. It is used for anorexia, vomiting and diarrhea caused by physical weakness, boredom, fatigue, sweaty symptoms, and loss of nasal function due to lack of energy. It is also used in cases of shortness of breath due to weakened lung function, and is also used for thirst and thirst without energy due to damage to the fluid due to the production of essence. With the action of anxiety, many dreams and sleeplessness, do not sleep, play well, strengthen the mental power, enhance the thinking and spiritual activities. If the energy is weak, causing symptoms of blood loss will give blood (氣血). In addition, due to renal function weakness is a tonic effect (음 症: erectile dysfunction) when the tonic effect.

The baekbokyeong (Poria cocas Wolf.) Used in the present invention is a medicinal mushroom that has been used as a herbal material since ancient times as a mushroom of the fungus fungi Democracy. Medicinal is the fungus that this mushroom makes on pine roots in the ground, and fruiting bodies rarely form on the surface of the fungus when it is exposed to the surface. The bacterium is a potato or oval lump with a diameter of 10-30 cm, the surface is dark brown, and is covered with a hard wrinkled shell. Meat is similar to semi-dried cheese and is white in color. When the tissue is viewed under a microscope, many granules are mixed into the mycelium pieces and severely refract light rays. This is a variation of mycelium, which exhibits a dextrin reaction that changes color to dark reddish brown in potassium iodide solution. The fruiting body of Bokryeong spreads flat on the fungal nucleus, having only 2 to 20 mm of depth and 0.5 to 2 mm of pores arranged on the surface, showing no mushrooms, and almost white in color. Diuretics, soothing agents, tonics, used for the treatment of edema, mental anxiety, palpitations, dyspepsia. In addition, it has detoxification effect, prevents freckles, and protects the skin in terms of beauty of skin, and recently, there is an antitumor effect on pakiman, a polysaccharide as one of its ingredients. The effect is expected more.

Cnidium officinale Makino used in the present invention is said to have an effect of vasodilation, anti-inflammatory, antipyretic, analgesic treatment, and the like has been used for the purpose of the treatment of blood, tonic, sedation, analgesic, etc. in Oriental medicine.

Benincase hispida cogniaux, used in the present invention, is also known as the Baekdongdae because it is a support and bears fruit on the vines and is initially turquoise and whitens as if it is frosted. Thicken the base, make the face beautiful, and remove the black freckles.

Prunus armeniaca L. used in the present invention eliminates the appearance of blemishes, and the celadon removes dead sesame seeds and spreads wrinkled flesh.

The honey used in the present invention is stored by converting the nectar and pollen collected from the flowers of various plants into their food, two monosaccharides, glucose and fructose, carbohydrate, It contains minerals, vegetable pigments, enzymes and pollen. More than 80% of honey's components are monosaccharides such as glucose and fructose. This ingredient has excellent hygroscopicity and forms a moisture film on the skin, which has a continuous moisturizing effect. Honey extract contains a large amount of these ingredients has a strong moisturizing effect.

Polygonnatum odoratum (MILLER) var. Pluriflorum Ohwi used in the present invention is a perennial herb of a monocotyledonous lily of the genus Liliumaceae. It grows in mountains and fields. The coarse fleshy rhizome is sideways and the stem has six ridges and the tip is slanted obliquely. The height is 30-60cm. The leaves are shifted and spread to one side. 5-10cm long, 2-5cm long, oval, without petioles. In June-July, 1 ~ 2 greenish white flowers, 15 ~ 20mm long, hang on the axilla, and the peduncles merge with each other at the bottom. There are 6 stamens, attached to the upper part of the tube, and there are small projections on the operating table. Anther is 4mm long and almost the same length as operating table. Fruits are round with berry and ripen black from September to October. Eat young leaves and rhizomes in spring. The crude oil of the herbal medicine is dried root stems, and in one shot, the root stems are used for the treatment of alternation, diabetes and heart failure. It is distributed in Korea, Japan, and China. On the back of the leaf, there are glass-like protrusions and flowers are 2 ~ 2.5cm in length, var. Thunbergii, and on the veins on the back of the leaf, there are many bumps, and 1 ~ 4 flowers are running on the leaf, var. Maximowiczii. , The leaves are 16cm long, 5cm butterfly, and 4 flowers are running each. P. koreanum, the whole is large and hairy on the back, and 2-5 flowers are called P. robustum.

As used in the present invention, Prunus armeniaca L. is also referred to as sky root roots, also known as cheonhwabun (千 花粉), stigma (천 名), cheonggwa (天 瓜) and eliminates wrinkles on hands and face, hemorrhage, Used to treat red blood, red Baekli.

As used in the present invention (Thladiantha dubia Bunge) is to multiply and brighten the face, passerby eliminates blemishes.

Prunus persica used in the present invention is a seed of Rosaceae larch, Prunus persica (L.) B., emulsion, amygdalin, linolenic acid Etc. are contained. When the peach ripens around July-August, the seeds are collected, the hard seed husks are broken, and the vines are taken out and used mainly as herbal medicines. It is an egg-shaped one-piece small lump, 1.2-2.0 cm long, 0.6-1.2 cm wide, 0.3-0.7 cm thick, wrapped in a reddish brown thin shell, and white inside. The taste is slightly bitter and water is added to smash the fragrance of benzaldehyde. Doin is used for menstrual pain, amenorrhea and postpartum abdominal pain when the bleeding is in the body. It is used for pain, anti-inflammatory, detoxification, and as a relieving agent. It is known to have an effect of stopping a cough. Herbal prescriptions that include this include Dokukseunggi-tang (桃核 承 氣 湯), Rhubarb Moranpitang (大黃 牡丹皮 湯), Kejijibokyeonghwan (桂枝 茯 笭 丸) and the like.

Peony (Paeonia moutan Sims, P.subfruticosa Makino) used in the present invention is native to western China and is believed to be a naturalized plant introduced in China due to the records of Queen Seondeok in the Silla period. It has strong cold resistance and is distributed throughout the country except North Hamgyong, but it is cultivated in Danyang-gun, Jewon-gun, and Hwasun-gun, Jeonnam. Perennial deciduous shrub, 1 ~ 1.5 m high. Leaves alternate, two-time exudates, egg-shaped or frenzied, and degenerative lobules are always divided into three, and lateral lobes are arranged in three rows. Flowers are single and large in size, with 5 sepals, 5 or more petals, and most of the cultivars are double-flowered, variegated, rose, red, purple, and white. There are several stamens, the operating table is red, pistil is 2 to 5, pistil is lobed, and the flower bed is cup-shaped. The outer surface is dark brown to dark red, and there are small oval side root marks on the side, and there are vertical wrinkles. The inner side is light brown to dark red and flat. The fracture surface is rough and white to pink powdery. It has an unusual smell, so it can be easily identified, the taste is slightly spicy and bitter. The white and paeonol are attached to the inner and curved sides, and the stronger the fresh scent, the better the product. A shrub with peonies, planted with flowers or herbs from various places. In the fall, roots are removed, the neck is removed and sunburned. Root bark and cores include the pheonol glycosides phenosides, pheonolides, peonyflorin, benzoic acid, and pheonol. It is used for autonomic nervous system relief as an antipyretic, antipyretic, analgesic, and anti-inflammatory agent. It is also used for menstrual irregularities and back pain. When used together with the skin and peach seeds (doin) increases the anti-inflammatory effect and blood purification. Peony Root bark of Paeonia suffruticosa A. Paeonol and its glycosides, paeonoside, paeonolide, and paeoniflorin. It is contained. This is an essential drug of gynecological drugs, sedative action, fever lowering action, pain relief action, anti-inflammatory action and bloody action and blood pressure lowering action has been reported. Bark skin is known to show anti-inflammatory effect and blood circulation improvement when used in combination with causality. On the other hand, the efficacy of the endothelial skin associated with cosmetics has a boil treatment effect by anti-inflammatory action, tyrosinase activity inhibitory effect, it is known to prevent skin aging by promoting blood circulation and anti-inflammatory action and astringent action.

Seisin (Aiasarum sieboldii Miquel) used in the present invention dried the roots of the Korean cephalopods and the cephalopods belonging to the group Aristolochiaceae and are commonly referred to as the cephalopods. Unevenly bent strings are 2 ~ 4cm long, 2 ~ 3mm in diameter, yellowish brown nodes with roots of about 15cm in length and 1mm in diameter. It smells and tastes spicy and slightly numbs the tongue. Its component contains l-sesamin, a lignan compound, 1.9-3.4% of essential oils, and essential oils include methyleugenol, palmitic acid, saprolol, pinene, cineol and eucarbon. There are antipyretic, soothing, analgesic, anti-inflammatory, anti-allergic, local anesthetic and fungicidal effects.

Bombyx mori Linn used in the present invention is a dry worm of the larva inoculated with the larvae of Bombycidae silkworm family (Beauveriabassiana (Bals.) Vuill.), Dried or limed and kneaded Bake and dry it as a medicine. The herbal name is called Batyticatus Bombycis, Corpus Bombycis. Baeksijam has been traditionally used for jinjing, soothing, swelling, and seedling (prime metabolism), and has been studied as a dermatitis treatment (Japanese Patent Application Laid-Open No. 5-246874).

Rehmannia glutinosa Libschitz var.purpurea Makino used in the present invention is a perennial medicinal plant with a ginseng family, whose roots are used as a medicinal herb in one shot. It is called nine times to make the dried 9 times repeatedly dried, the best effect of the medicine. Lower back and knee pain, painful symptoms, dysmenorrhea, dizziness, etc. to treat the black hair is effective. In addition, it prevents skin keratinization, softens the skin, softens keratinocytes, and promotes blood circulation.

Various herbal ingredients in the 'Gagamcheon forbidden', 'Gagamgyeongokgo' and 'Gagamhongoksan' herbal extract mixtures provided by the present invention can be obtained by using various extraction methods known in the art, The content and the like can also be arbitrarily selected according to the purpose.

Preferably, it can be obtained using one or more solvents selected from the group consisting of water, ethanol, methanol, butanol, ethyl acetate, butylene glycol, hydrous propylene glycol, hydrous glycerin, hydrous ethanol, hydrous butylene glycol. In addition, it is preferable to extract by adding 5-20 times the volume amount of solvent at this time.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can obtain a herbal extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

The herbal mixture extract may be added to a conventional cosmetic composition to prepare a cosmetic composition for preventing skin aging.

The content of the herbal extract mixture should be appropriately selected according to the use of each cosmetic composition, the formulation of the cosmetic composition and the skin condition. The content of the herbal extract mixture may be contained in an amount of 0.001-20% by weight, preferably 1-10% by weight, based on the total weight of the cosmetic composition. If the content of the mixture is less than 0.001% by weight relative to the total weight, it is difficult to expect the desired effect in the present invention, if it is more than 20% by weight may cause problems in formulation stability.

'Gagamcheon Forbidden' and 'Gagamgyeongokgo' and 'Gagamhongoksan' provided by the present invention show much better cell proliferation and metabolic activation ability than a single application when applied simultaneously, and synergy by applying three mixed extracts simultaneously It is possible to maximize the skin cell activity by the effect. In this case, 'Gagamcheon Geumdan', 'Gagamgyeong Okgo' and 'Gagam Hongoksan' are preferably used in a weight ratio of 10:10:80 to 80:10:10.

The cosmetic composition of the present invention may include ingredients conventionally used in cosmetic compositions in addition to the herbal extract as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments, flavors, or carriers. It may include.

Carriers, excipients, and diluents that may be included in the composition containing the extract of the present invention as an active ingredient include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate , Gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is preferable to use diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants that are commonly used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are preferably mixed. In addition to simple excipients, lubricants such as magnesium styrate talc may also be used. Oral liquid preparations include suspending agents, liquid solutions, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories, and the like. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition of the present invention may further comprise a component selected from the group consisting of water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, the cosmetic composition of this invention can also mix | blend the other component normally mix | blended with a cosmetic composition as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cold depressants, delimiters, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyltrimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, In addition, any of the above components can be mix | blended within the range which does not impair the objective and effect of this invention.

Cosmetic compositions according to the invention can be prepared in any formulations conventionally prepared in the art, for example solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing It may be formulated as a cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, spray, and the like, but is not limited thereto. More preferably, it may be prepared in a formulation such as lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, and the like.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan may be used.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

Skin anti-aging cosmetic composition according to the present invention is characterized by having a cell proliferation and metabolic activity effect. The present invention is to increase the turn-over of the cell from the above characteristics to regenerate aging skin cells, increase the synthesis of extracellular epilepsy produced in the cell extracellular epilepsy directly involved in skin elasticity, metabolism, vitality, etc. The increase in the amount of the compound has the effect of preventing skin aging through increased skin elasticity, promote skin metabolism and enhance skin vitality.

The cosmetic composition for preventing skin aging according to the present invention is characterized by having a free radical scavenging effect. The present invention has the effect of preventing the skin aging through the skin wrinkle suppression and improvement, skin elasticity increase from the above characteristics.

Skin anti-aging cosmetic composition according to the invention is characterized in that it has a collagen production promoting effect. The present invention has the effect of preventing the skin aging through the skin wrinkle suppression and improvement, skin elasticity increase from the above characteristics.

The present invention has the effect of preventing skin aging through the inhibition and improvement of skin wrinkle generation, increase skin elasticity, promote skin metabolism and enhance skin vitality from the above characteristics bar term "anti-aging skin" in the present invention is wrinkle improvement It is interpreted as meaning including improving skin elasticity, promoting skin metabolism and enhancing skin vitality. The term "anti-aging of skin" also has the meaning of preventing, improving and treating skin aging.

According to another embodiment of the present invention, dried dried white paper, ginkoin, cheongung, dongle, sessin, earthen root, doin, neck skin, shinyi, saengsonghyang and Baekjap in a solvent and warmed at 60 ~ 80 ℃ for 2 to 5 hours After extraction, the mixture was filtered, cooled, and aged for 4 to 14 days at 5-15 ° C., followed by filtration to prepare a herbal extract mixture A. The dried ginseng, turmeric and honey were put in a solvent and warmed at 60 to 80 ° C. for 2 to 5 hours. After extraction, the resultant was filtered, cooled and aged for 4-14 days at 5-15 ° C., followed by filtration to prepare a herbal extract mixture B, and dried dried almonds, fruit roots and Baekbongryeong in a solvent at 2˜5 ° C. at 60 ° C. to 80 ° C. After extracting by heating for a period of time, filtered, cooled and aged for 4-14 days at 5-15 ℃ and then filtered to prepare a crude drug extract mixture C, a method for producing a skin anti-aging cosmetic composition for mixing the herbal extract mixture A, B, C To provide.

Cooling is preferably performed at room temperature after extraction of the dried herbal ingredients into a solvent, but is not limited thereto.

The order of preparing the herbal extract mixtures A, B and C is not limited.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

White ginseng, Dongguin, Cheongung, Dongle, Seshin, Earthen root, Doin, Peelwood, Sinyi, Gamsonghyang and Baekjajam according to the present invention as described above ('Gagamcheoncheondan') and made of ginseng, jihwang and honey Skin anti-aging cosmetic composition containing a mixed extract ('gagamyeongokgo'), almonds, fruit roots and baekbongyeong ('gagamhongoksan') as an active ingredient can be used safely without side effects on the skin. In addition, it prevents and improves skin aging by improving cell proliferation and metabolic activity, free radical scavenging, and improving skin wrinkles and skin elasticity by promoting collagen production.

Preparation Examples 1-10: Preparation of Gagamcheon Forbidden Mixture Extract

    Dried white paper, Dongguin, Cheongung, Dongle, Sessin, Earthen root, Doin, Bark skin, Sinyi, Gamyang incense, Baeksijam in distilled water 5kg and extracted by heating at 70 ℃ for 3 hours using an extractor equipped with cooling condenser After filtering with a mesh filter cloth (whatman), cooled to room temperature and left to mature for 7 days at 5-15 ℃, and filtered with a 0.45 μm filter to obtain 4.3 kg of extract.

menstruum Yield (kg) Example 2. ethanol 4.2 Example 3. Methanol 4.3 Example 4. Butylene glycol 4.0 Example 5. Propylene glycol 4.1 Example 6. glycerin 3.9 Example 7. Water Butylene Glycol (30 w / w%) 4.1 Example 8. Hydrous Propylene Glycol (30 w / w%) 4.1 Example 9. Hydrous ethanol (30 w / w%) 4.4 Example 10. Hydrous ethanol (70 w / w%) 4.3

Preparation Examples 11-20 : Preparation of Gagamgogo Mixed Extract

    Dry ginseng, turmeric and honey in 2kg of distilled water and extracted by heating at 70 ℃ for 3 hours using an extractor equipped with cooling condenser, filtered with 400 mesh filter cloth (whatman) and cooled to room temperature for 7 days at 5-15 ℃. After standing to age, it was filtered through a 0.45μm filter to obtain 1.6kg of extract.

menstruum Yield (kg) Example 12. ethanol 1.8 Example 13. Methanol 1.7 Example 14. Butylene glycol 1.7 Example 15. Propylene glycol 1.8 Example 16. glycerin 1.5 Example 17. Water Butylene Glycol (30 w / w%) 1.7 Example 18. Hydrous Propylene Glycol (30 w / w%) 1.6 Example 19. Hydrous ethanol (30 w / w%) 1.7 Example 20. Hydrous ethanol (70 w / w%) 1.8

Preparation Examples 21-30: Preparation of gagamhongoksan mixed extract

    Dry dried almonds, fruit roots and Baekbongryeong in 2kg of distilled water and extracted by heating at 70 ° C for 3 hours using an extractor equipped with a cooling condenser. After standing for one day and aged, the resultant was filtered with a 0.45 μm filter to obtain 1.4 kg of extract.

menstruum Yield (kg) Example 22. ethanol 1.5 Example 23. Methanol 1.3 Example 24. Butylene glycol 1.4 Example 25. Propylene glycol 1.2 Example 26. glycerin 1.3 Example 27. Water Butylene Glycol (30 w / w%) 1.4 Example 28. Hydrous Propylene Glycol (30 w / w%) 1.4 Example 29. Hydrous ethanol (30 w / w%) 1.4 Example 30. Hydrous ethanol (70 w / w%) 1.5

Experimental Example  1-7

Experimental Examples 1 to 7 below were prepared by experimenting the crude drug mixture extract extracted with a solvent of 30% ethanol under reduced pressure and concentration using an evaporator to prepare a powder.

Experimental Example  1. Cytotoxicity

Cytotoxicity in monolayer cell cultures was measured for the mixed extracts.

Experiment method

MTT assay [Reference: Mossman T. (1983) Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55 ~ 63] is a standard colorimetric assay that is widely used for cell proliferation and toxicity by measuring the number of living cells. MTT (3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide, Thiazolyl Blue Tetrazolium Bromide), a yellow water-soluble substrate in mitochondria of living cells, is also known as dehydrogenase (succinate dehydrogenase or mitochondrial dehydrogenase). Is reduced to a water-insoluble blue formazan derivative. The resulting formazan derivative is dissolved by adding a dissolving agent (usually using DMSO (dimethylsulfoxide)) and measuring the absorbance. The resulting formazan derivative is dissolved in DMSO and the optical density is measured. The absorbance measured is linearly correlated with the number of living cells.

More specifically, human fibroblasts cell line (ATCC, CRL-2310) was inoculated in a 24well plate at a concentration of 1x10 ⁴ cells / ml. Medium was used DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 10% bovine serum. 24 hours after inoculation, it was replaced with DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 2% of bovine serum, and 10 μl of test sample was added at a concentration of 50 μg / ml. Incubated in a CO ₂ incubator. After the incubation, the supernatant was removed and washed again by adding 200ul of PBS (phosphate buffered saline), 1.0ml of MTT solution was added per well, MTT was removed after 4 hours, and DMSO (Dimethy 1 Sulfoxide) was added 1.0ml per well. After incubation at 37 ℃ overnight, the absorbance was measured at a wave length of 570nm using an ELISA reader (sunrise, Austria). The results are shown in Table 1.

Table 1. Cytotoxicity in Monolayer Cell Culture of Mixed Extracts

sample Concentration (ug / ml) Cell survival rate (%) Gagamcheon Forbidden 0.1 101 One 100 10 103 50 100 100 102 Gagamgookgo One 103 10 102 50 100 100 101 200 101 Gagam Hongoksan One 103 10 104 50 101 100 103 200 100

As shown in the results of Table 1, the Gagamcheon forbidden, Gagam Gyeongokgo, Gagam Hongoksan all have a cell survival rate of 100% or more, it can be seen that the safety is very excellent.

Experimental Example  2. Free radical  Elimination Experiment (1)

The hydroxyl radical scavenging effect of the mixed extract was measured.

Experiment method

Human fibroblasts are placed on the well plate at 2 × 10 ⁵ cells / well and 0.2 ml of EMEM medium (Eagle's minimum essential medium, BRL , USA ) containing 10% FBS is incubated in a CO ₂ incubator for 24 hours. The mixed extract dissolved in DMSO is treated and 30mM H ₂ O ₂ is added to induce hydroxyl radicals, followed by incubation for 24 hours. Then, MTT assay is performed to determine the growth rate of the cultured cells. In MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed, and 0.5 ml of DMSO was added. Formazan was measured using an ELISA reader (sunrise, Austria) to measure absorbance at 570 nm. Was performed. As a positive control, EGCG (epigallocatechin gallate) with excellent radical scavenging ability was used. After absorbance measurement for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 1 below and the results are shown in Table 2 below.

Equation 1

Hydroxyl radical inhibition effect (%) = ((absorbance of experimental group) / (absorbance of control group)) x100

Experimental group: sample treatment group

Control group: sample untreated group (all other experimental conditions are the same)

        Table 2. Hydroxyl Radical Scavenging Effects on Mixed Extracts

sample density( ug Of ml ) Hydroxyl radical scavenging rate (%) Gagamcheon Forbidden 0.1 15 One 34 10 56 50 79 100 95 Gagamgookgo One 10 10 25 50 42 100 53 200 76 Gagam Hongoksan One 12 10 27 50 41 100 49 200 56 Positive control group (EGCG) 10 67

As can be seen from the results shown in Table 2, the herbal extract mixture of the present invention was confirmed that the hydroxyl radical scavenging ability is very excellent, and as the concentration increases, the radical scavenging ability is further increased.

Experimental Example  3. Free radical  Elimination effect experiment (2)

The peroxide radical scavenging effect (antioxidant effect) was measured for the mixed extract.

Experiment method

Human fibroblasts are placed on the well plate at 2 × 10 ⁵ cells / well, covered with 0.2 ml of EMEM medium containing 10% FBS, and incubated in a CO ₂ incubator for 24 hours. This is treated with a mixed extract dissolved in DMSO and incubated for 30 minutes. 10 µl of 1.5 mM xanthine and 10 µl of 0.25 U xanthine oxidase were added to the cultured cells to generate peroxide radicals, and then cultured in a CO ₂ incubator for 24 hours. MTT assay is performed to determine the growth rate of the cultured cells. MTT assay was performed by adding 0.1 ml of MTT solution to the cultured cells, incubating for 4 hours, removing the medium and adding 0.5 ml of DMSO, and measuring the absorbance at 570 nm using ELISA reader (sunrise, Austria). It was. As a positive control, EGCG (epigallocatechin gallate) with excellent radical scavenging ability was used. After measuring the absorbance for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below and the results are shown in Table 3 below.

Equation 2

Peroxide radical inhibition effect (%) = ((absorbance of experimental group) / (absorbance of control group)) x100

Experimental group: sample treatment group

Control group: sample untreated group (all other experimental conditions are the same)

        Table 3. Peroxide Radical Scavenging Effects on Mixed Extracts

sample density( ug Of ml ) Peroxide radical scavenging rate (%) Gagamcheon Forbidden 0.1 5 One 13 10 37 50 56 100 79 Gagamgookgo One 10 10 16 50 19 100 28 200 55 Gagam Hongoksan One 8 10 11 50 23 100 27 200 49 Positive control group (EGCG) 10 43

As can be seen through the results shown in Table 3, the herbal extract mixture of the present invention was very excellent peroxide radical scavenging ability, it was confirmed that the radical scavenging ability is increased as the concentration increases.

Experimental Example  4. Promotion of cell metabolism activity and synergy effect experiment

The metabolic activity and synergistic effect of the mixed extract was measured.

Experiment method

This experiment is to compare the cell proliferation ability of FBS (fetal bovine serum) containing nutrients and various hormones needed for cell growth with added mixed extract instead. The effect on proliferation can be seen.

Fibroblasts from neonatal foreskin tissue were placed in a 24-well plate at 2 × 10 ³ cells per well and incubated for 24 hours. One side of the mixed extract in DMEM medium and 10% FBS (fetal bovine serum) in DMEM medium is added to the control. After incubation for 4 days, MTT assay is performed to determine the proliferation rate of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed, and 0.5 ml of DMSO was added thereto. After measuring the absorbance for the experimental group and the control group, the cell metabolic activity was obtained using Equation 3 below and the results are shown in Table 4 below.

Equation 3

Cell metabolic activity promoting effect of the experimental group (%)

= ((Absorbance of experimental group) / (absorbance of negative control group)) x100

Cell metabolic activity promoting effect of the control group (%)

= ((Absorbance of control) / (negative control)) x100

Experimental group: FBS 0% + sample treatment group

Control group: FBS 10% + sample untreated group (all other experimental conditions are the same)

Negative Control: FBS 0%

        Table 4. Effect of promoting cell metabolism activity on mixed extract

     Example   Final concentration of the sample in the reaction solution (µg / ml) Promote cellular metabolic activity (proliferation) effect (%) Control (FBS added) Mixed extract added Gagamcheon Forbidden 10 109 105 50 118 116 100 129 127 Gagamgookgo 50 118 109 100 129 114 200 135 121 Gagam Hongoksan 50 118 108 100 129 110 200 135 114 Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan 50 118 115 100 129 127 200 135 138

As can be seen from the results shown in Table 4, the addition of the herbal extract mixture of the present invention showed the same effect as promoting the cell metabolism activity when FBS was added, and as the concentration increases the effect of promoting cell metabolism activity The results were further increased. In addition, the addition of the Gagamcheon forbidden, Gagam Gyeongok, and Gagamhongoksan showed a more enhanced effect of promoting cell metabolism than when added respectively.

Experimental Example  5. Cell proliferation effect and synergy effect experiment

Cell growth and synergy were measured for the mixed extract.

Experiment method

100 μl of 5% young calf serum (FBS) solution was added to 96 microwell plate (96 Corning), and then dispersed in DMEM medium containing 2% FBS. MTT assay was performed to evaluate the degree of cell proliferation. Fibroblasts from neonatal foreskin tissues were dispersed in 100 ul DMEM medium per well and cultured for 7 days. After the incubation, the supernatant was removed and washed again by adding 200ul of 5% PBS (phosphate buffered saline), and 150ul of DMSO was added per well, and then shaken for 10 minutes. At this time, the experiment was performed in the same manner without adding the mixed extract as a control group and the cell proliferation effect was compared from the difference in relative absorbance. After measuring the absorbance for the experimental group and the control group, using the following equation 4 to obtain the cell proliferation effect is shown in Table 5 below.

Equation 4

Cell proliferation effect (%) = ((absorbance of experimental group) / (absorbance of control group)) x100

Experimental group: sample treatment group

Control group: sample untreated group (all other experimental conditions are the same)

        Table 5. Cell Proliferation and Synergy Effects on Mixed Extracts

sample Concentration (ug / ml) Cell proliferation effect (%) Gagamcheon Forbidden 0.1 105 One 111 10 119 50 123 100 134 Gagamgookgo One 103 10 109 50 118 100 123 200 126 Gagam Hongoksan One 104 10 114 50 120 100 125 200 127 Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan 50 135 100 148 200 152

Control (2% FBS Medium): 100%

As can be seen from the results shown in Table 5, the experimental group treated with the herbal extract mixture shows a very high fibroblast proliferation effect compared to the control group without the herbal extract mixture. In addition, the experimental group treated with three herbal extract mixtures showed a superior cell proliferation effect, compared to the experimental group treated with Gagamcheon forbidden and Gagamgyeongokgo gagamgamoxane, respectively.

Experimental Example  6. type  Ⅰ Collagen Biosynthesis

The type I collagen biosynthesis was measured for the mixed extract. (Kanayama. N., and Terao, T. 1992, Gynecol. Obstet. Invest. 34, 24-26)

Experiment method

Procollagen type I C-peptide ELA kit (TaKaRa, cat. # MK101) was used to measure the amount of collagen synthesis released into the media from fibroblasts. Before using KIT, human fibroblasts cell line (ATCC, CRL-2310) was inoculated in a 24 well plate (24 well plate, Corning) at a concentration of 1 × 10 ⁴ cells / ml. Medium was used DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 10% FBS (bovine serum). After 24 hours of inoculation, 10 µl of the test sample was added to a concentration of 50 µg / ml and incubated in a 37%, 5% CO ₂ incubator for 24 hours. The cultured medium was transferred to an E-tube and centrifuged for 30 seconds at 15,000 rpm. The supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen type I C-peptide (PIP) using a kit (Takara, Japan).

 After implementation, the absorbance was measured at 450 nm with ELISA reader (sunrise, Austria). At this time, the experiment was conducted in the same manner as the positive control with the addition of vitamin C to compare the amount of collagen production from the difference in relative absorbance. Vitamin C used as a control is a substance that promotes collagen synthesis by helping the activity of fibroblasts. The results are shown in Table 6.

Table 6. Type I Collagen Biosynthesis of Mixed Extracts

sample Concentration (ug / ml)  PIP (ng / ml) Synthetic growth rate (%) Gagamcheon Forbidden 0.1 250 16 One 329 21 10 371 33 50 396 42 100 462 48 Gagamgookgo One 223 10 10 250 16 50 319 28 100 337 35 200 372 41 Gagam Hongoksan One 264 17 10 294 18 50 327 20 100 351 27 200 368 39 Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan 50 388 40 100 462 48 200 518 59 Control group (vitamin C 50µg / ml) 391 41 No treatment group 203 3

As can be seen from the results shown in Table 6, the experimental group treated with the herbal extract mixture shows a very high type I collagen biosynthesis compared to the control group without the herbal extract mixture. In addition, it shows the same effect as the control group treated with Vine C. In addition, the experimental group treated with the three herbal extract mixtures showed better type I collagen biosynthesis than the experimental group treated with Gagamcheon Forbidden and Gagam Gyeongokgo. As the concentration of herbal extract mixture increases, the amount of type I collagen biosynthesis increases.

Experimental Example  7. Type  Ⅰ Collagen mRNA  Expression level investigation

RT-PCR (Reverse Transcription-Polymerase Chain Reaction) was performed to measure the expression level of the type I collagen gene with respect to the mixed extract obtained from the above example. Human Normal Fibroblasts (ATCC-2706) in DMEM medium (Gibco / BRL) containing 10% bovine serum (Gibco / BRL) at a density of 1 × 10 ⁶ in a 60 mm culture dish in 37 ° C., 5% CO ₂ incubator. Raised for a day. Thereafter, the cells were exchanged with a medium containing 2% serum, and samples were added at different concentrations and incubated at 37 ° C for 24 hours. Then, total RNA was isolated from the cells by guanidium thiocyanate-phenol-chloroform extraction using Trizole reagent (Gibco / BRL). In order to perform RT-PCR, the RNA obtained by the above method was treated with RNA degrading enzyme-free DNAase (RNase-free DNase, Promega) at 37 ° C for 30 minutes to remove DNA contamination, phenol / chloroform extraction method and ethanol. Precipitation gave pure RNA.

RT-PCR was performed using 1 μg of RNA using an All in one RT / PCR kit (Superbio). Beta-actin was used as a control, and the primer of type I collagen used in RT-PCR was composed of SEQ ID NO: 1 and SEQ ID NO: 2, and the primer of beta-actin was composed of SEQ ID NO: 3 and SEQ ID NO: 4 It is. The reaction conditions were type I collagen at 94 ° C for 1 minute, 62 ° C for 1 minute, 72 ° C for 1 minute, and 30 cycles, for beta-actin at 95 ° C for 1 minute, 62 ° C for 1 minute, 72 ° C for 1 minute, and 22 cycles for PCR. The product was quantified by a gel image analyzer (Biorad) by electrophoresis on a 1.5% (w / v) agarose (agarose, Sigma) gel. Vitamin C used as a control is a substance that promotes collagen synthesis by helping the activity of fibroblasts. The results are shown in Table 8.

Table 7. Primers

SEQ ID NO: 1 5'-CTGGCAAAGAAGGCGGCAAA-3 ' SEQ ID NO: 2 5'-CTCACCACGATCACCACTCT-3 ' SEQ ID NO: 3 5'-GAGACCTTCAACACCCCAGCC-3 ' SEQ ID NO: 4 5'-GGCCATCTCTTGCTCGAAGTC-3 '

Table 8.

sample Type I Collagen Expression (%) 100 μg / ml 200 μg / ml Gagamcheon Forbidden 139.28 156.87 Gagamgookgo 112.92 111.62 Gagam Hongoksan 109.4 110.7 Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan 145.21 189.54 Control group (vitamin C 100 μg / ml) 131.1 156.3 No treatment group 100 100

As can be seen from the results shown in Table 8, compared to the control group not treated with the herbal extract mixture, the experimental group treated with the herbal extract mixture shows a very high type I collagen expression. In addition, it shows the same effect as the control group treated with Vine C. In addition, the experimental group treated with three herbal extract mixtures showed more superior type I collagen expression than the experimental group treated with Gagamcheon Forbidden and Gagam Gyeongokgo Gagam Hongoksan, respectively. As the concentration of herbal extract mixture increases, the amount of type I collagen expression increases. This result is also consistent with the result shown in Table 6 above.

Formulation example  And Comparative example

In the cosmetic composition having a composition ratio of the following table, a formulation example including the inventive herbal extract mixture and a comparative example without the herbal extract mixture were prepared.

Formulation example  And Comparative example  One.

A flexible lotion (skin) was prepared according to a conventional method as shown in the table below.

number Raw material Formulation Example 1 (% by weight) Comparative Example 2 (wt%) One Mixed extract (Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan 2.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene (60) Cured Castor Oil 1.00 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Remaining amount Remaining amount system 100.0 100.0

Formulation example  And Comparative example  2.

 Nutrients (lotions) were prepared according to a conventional method as shown in the following table.

number Raw material Formulation Example 2 (wt%) Comparative Example 2 (wt%) One Mixed Extract (Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan) 2.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 10.0 10.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.3 0.3 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount system 100.0 100.0

Formulation example  And Comparative Example 3

 Nutritious cream was prepared according to a conventional method as shown in the following table.

number Raw material Formulation Example 3 (wt%) Comparative Example 3 (wt%) One Mixed Extract (Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan) 5.0 - 2 Cytostolol 4.0 4.0 3 Polyglyceryl 2-oleate 3.0 3.0 4 Ceramide 0.7 0.7 5 Ceteares-4 2.0 2.0 6 cholesterol 3.0 3.0 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 22.0 22.0 10 Carboxy Vinyl Polymer 0.5 0.5 11 Triethanolamine 0.5 0.5 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount system 100.0 100.0

Formulation example  And Comparative example  4.

 Essences were prepared according to conventional methods as shown in the table below.

number Raw material Formulation Example 4 (wt%) Comparative Example 4 (wt%) One Mixed Extract (Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan) 7.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 15.0 15.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount system 100.0 100.0

Formulation example  And Comparative example  5.

 Emulsified foundations were prepared according to conventional methods as shown in the table below.

number Raw material Formulation Example 5 (wt%) Comparative Example 5 (wt%) One Mixed Extract (Gagamcheon Forbidden + Gagam Gyeongokgo + Gagam Hongoksan) 2.0 - 2 Beeswax 2.0 2.0 3 Pyromethicone 2.0 2.0 4 Liquid paraffin 5.0 5.0 5 Squalane 5.0 5.0 6 Stearic acid 2.0 2.0 7 Lipophilic monostearic acid glycerin 3.0 3.0 8 Caprylic / Capric Triglycerides 4.0 4.0 9 glycerin 4.0 4.0 10 Propylene glycol 3.0 3.0 11 Butylene glycol 3.0 3.0 12 Triethanolamine 1.0 1.0 13 Aluminum Magnesium Silicate 0.5 0.5 14 Pigment 12 12 15 antiseptic a very small amount a very small amount 16 Spices a very small amount a very small amount 17 Purified water Remaining amount Remaining amount system 100.0 100.0

Test Example  1. Measurement of skin elasticity improvement effect by reducing wrinkle depth

Experiment method

In order to test the effect of the mixed extract on the skin wrinkles, Formulation Example 3 was divided into two groups of 20 women (average age 42 years) and applied twice a day and then morning and evening to the left eye edge. Nourishing cream of Comparative Example 3 was applied to the right eye edge and used as a control. The skin wrinkle depth of the skin surface after 15 and 30 days of application was measured by Cutometer SEM 474 (Courage + Khazaka electronic GmbH. Germany). Skin elasticity measurement by Cutometer SEM 474 is a measure of the depth of wrinkles, the smaller the value is the better elasticity. The elasticity value was shown as a percentage decrease compared to the control, and the average value of 20 subjects is shown in Table 9.

Experiment result

table. 9

sample Skin elasticity (wrinkle depth reduction) effect (%) 0 days 15th 30 days Formulation Example 3 0 5.2 18.9 Comparative Example 3 0 0.5 1.2

As shown in the results of Table 9, the skin elasticity (decrease in wrinkle depth) of the embodiment containing the herbal extract mixture was increased by more than 10 times compared to the comparative example, 30 days after 15 days without containing the herbal mixture extract Poetry was found to increase more than 18 times.

Experimental Example 2. Experiment to confirm the effect of improving skin condition

In order to confirm the skin condition improvement effect during the skin coating on the essence of Formulation Example 4 and Comparative Example 4, the following clinical confirmation experiment was carried out. Healthy females aged 36 to 69 years old, divided into two groups of 30 people, the essence of Formulation Example 4 of Formulation Example 4 on the left side of the face, and the essence of Comparative Example 4 on the right side of the face. After applying for 4 weeks, the degree of improvement of skin condition was measured to confirm the improvement of skin condition. (A) Improvement effect on skin elasticity and sagging, and (B) Improvement effect on wrinkles and fine lines were judged by the following ratings, and evaluated by an average score of 30 people. The test results are shown in Table 10.

Table 10.

(A) Rating criteria for improvement effect on skin elasticity and sagging

Was greatly improved  3 points Was improved  2 points Slightly improved  1 point No improvement  0 points Worsened -1 point

(B) Rating criteria for improvement effect on wrinkles and fine lines

Was greatly improved  3 points Was improved  2 points Slightly improved  1 point No improvement  0 points Worsened -1 point

(C) Test results

sample (A) skin elasticity, sagging (B) wrinkles, fine lines Formulation Example 4 63 60 Comparative Example 4 32 29

As shown in the results of Table 10, in the group using Formulation Example 4, a much better skin improvement effect was confirmed in comparison with the group using the comparative example in both (A) and (B).

As described above, the mixed extract ('gagamcheon forbidden') and ginseng, jihwang and baekji, dongwain, cheonggung, roundle, sessin, earthen root, doujin, mokdanpi, shinyi, gamsonghyang and baekgangjam according to the present invention as described above Skin anti-aging cosmetic composition containing a mixed extract consisting of honey ('gagamyeongokgo'), almonds, fruit roots and baekbongyeong ('gagamhongoksan') as an active ingredient can be used safely without side effects on the skin. In addition, it prevents and improves skin aging by improving cell proliferation and metabolic activity, free radical scavenging, and improving skin wrinkles and skin elasticity by promoting collagen production.

<110> korea University industry and academy collaboration foundation <120> Cosmetic composition for preventing skin <160> 4 <170> KopatentIn 1.71 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> RT-PCR primer 1 for type 1 collagen <400> 1 ctggcaaaga aggcggcaaa 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> RT-PCR primer 2 for type 1 collagen <400> 2 ctcaccacga tcaccactct 20 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> RT-PCR primer 3 for beta-actin <400> 3 gagaccttca acaccccagc c 21 <210> 4 <211> 21 <212> DNA <213> Artificial Sequence <220> RT-PCR primer 4 for beta-actin <400> 4 ggccatctct tgctcgaagt c 21  

Claims (12)

A cosmetic composition for preventing skin aging comprising, as an active ingredient, a herbal extract mixture consisting of white paper, gingiwine, cheonggung, roundle, sessin, earthworm, doujinshi, bark skin, shinyi, saengsonghyang and napjap. A cosmetic composition for preventing skin aging, comprising a herbal extract mixture consisting of ginseng, sulfur, and honey as an active ingredient. A cosmetic composition for preventing skin aging, comprising as an active ingredient a herbal extract mixture consisting of almonds, fruit roots and baekbongryeong. A skin anti-aging cosmetic composition comprising all of the herbal extract mixtures of claim 1 as an active ingredient. The method of claim 1, wherein the herbal extract mixture is water, ethanol, methanol, butanol, ethyl acetate, butylene glycol, hydrous propylene glycol, hydrous glycerin, hydrous ethanol and hydrous butylene glycol A skin composition for preventing skin aging, characterized in that the extraction by adding a volume of 5-20 times one or more solvents selected from the group consisting of. The method of claim 4, wherein the weight ratio of the herbal extract mixture of claim 1, the herbal extract mixture of claim 2, and the herbal extract mixture of claim 3 is 10:10:80 to 80:10:10, respectively. Cosmetic composition. The cosmetic composition for preventing skin aging according to claim 4, wherein the herbal extract mixture is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition. The cosmetic composition for preventing skin aging according to any one of claims 1 to 4, wherein the cosmetic composition has a formulation of a lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence or a pack. The cosmetic composition for preventing skin aging according to any one of claims 1 to 4, wherein the cosmetic composition has a cell proliferation and metabolic activity effect. The cosmetic composition for preventing skin aging according to any one of claims 1 to 4, wherein the cosmetic composition has a free radical scavenging effect. The cosmetic composition for preventing skin aging according to any one of claims 1 to 4, wherein the cosmetic composition has a collagen production promoting effect. Put dried white paper, Dongguin, Cheongung, Dongle, Sessin, Earthgeun, Doin, Bark skin, Sinyi, Gamsonghyang and Baeksijam in a solvent and extract it by heating it at 60 ~ 80 ℃ for 2 ~ 5 hours, then filtered and cooled. After fermentation at -15 ℃ for 4-14 days, the crude drug extract mixture A was prepared by filtration, and dried ginseng, turmeric and honey were put in a solvent, extracted by heating for 2-5 hours at 60-80 ℃, filtered and cooled. After fermentation at 5-15 ° C. for 4-14 days, the crude drug extraction mixture B was prepared by filtration. After aging for 4 to 14 days at 5-15 ℃ and filtered to produce a herbal extract mixture C, the herbal extract mixture A, B, C method for producing a cosmetic composition for preventing skin aging.