KR20200057677A - Composition for improving skin conditions comprising artemisiae annuae herba, centella asiatica and green tea extract or fraction thereof and method for improving skin conditions using the same - Google Patents

Abstract

The present invention relates to a composition for improving skin conditions, containing a complex extract of two or more selected from a group consisting of Artemisia annua, Centella asiatica, and green tea as an active component. Especially, the present invention provides a composition for improving skin conditions which contains a complex extract of two or more selected from the group consisting of Artemisia annua, Centella asiatica, and green tea as an active component, thereby having an effect of improving skin by synergy effects between extracts and/or between active components in the extract or by a collaboration thereof, and improving durability of effects.

Description

Composition for improving skin condition containing a mixture extract of Cheongho, Byeongpung and green tea or a fraction thereof as an active ingredient and a method for improving skin condition using the same TECHNICAL FIELD METHOD FOR IMPROVING SKIN CONDITIONS USING THE SAME}

The present invention relates to a composition for improving skin condition containing a mixed extract of Cheongho, Yeungpung and green tea or a fraction thereof as an active ingredient and a method for improving skin condition using the same. Specifically, the present invention improves the skin texture for improving skin texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation, which contains a mixed extract of Cheongho, Centella and Green Tea as an active ingredient. It relates to a cosmetic composition, a quasi-drug composition, or a food composition.

In addition, the present invention relates to a pharmaceutical composition for the prevention or treatment of skin diseases containing a mixed extract of Cheongho, Yeolpung and green tea or a fraction thereof as an active ingredient.

In addition, the present invention relates to a method for improving skin conditions or preventing or treating skin diseases using the compositions.

Cosmetics can be largely divided into basic cosmetics for nourishing the skin and color cosmetics in which pigments that are not harmful to the human body are mixed to express the contours individually. In particular, basic cosmetics include latex, lotion, skin, lotion, etc., and most women and men use basic cosmetics. These basic cosmetics function to prevent skin troubles by forming a thin protective film while calming the damaged skin due to cleansing, as well as cleansing the skin by removing dead skin and impurities of the skin, and controlling oil and moisture in a balanced manner.

In addition, in recent years, as the income increases, the standard of living has improved, making more investments in cosmetics to enhance one's image. In addition, as the elderly population increases, interest and demand for functional cosmetics related to anti-aging, wrinkle improvement, whitening, and UV protection are increasing. However, since the composition of these basic cosmetics or functional cosmetics is mostly made of chemicals, skin troubles often occur when applied to sensitive skin.

On the other hand, inflammation is a series of defenses aimed at minimizing the reaction and restoring the damaged area to the original state when cells or tissues are damaged by any cause.It causes nerves and blood vessels, lymphatic vessels, fluid reactions, and cellular reactions. It causes pain, swelling, redness, and fever, causing dysfunction. Causes of inflammation include physical factors such as trauma, frostbite, burns, and radioactivity, chemical factors such as acids, and immunological factors caused by antibody reactions, and also caused by blood vessels and hormonal imbalance. do. Vascular expansion occurs due to various chemical mediators secreted by cells damaged by external stimulation, and as permeability increases, antibodies, complements, plasma, and phagocytic cells crowd into the inflammatory site. This phenomenon causes erythema.

Anti-inflammatory drugs that act to remove inflammatory sources, reduce biological reactions, and symptoms to eliminate inflammation. Materials that have been used for anti-inflammatory purposes to date include non-steroidal drugs such as flufenamic acid, ibuprofen, benzydamine, and indomethacin, and prednisolone as a steroid system. , Dexamethasone (dexamethasone), and the like, but the safety of the skin, or when containing cosmetics, most of them have problems in terms of stability, so their use is limited.

Therefore, in recent years, it is excellent in moisturizing, anti-aging, antioxidant, and anti-inflammatory effects, and thus improves the skin condition or prevents skin aging or skin damage. A lot of research is going on. However, in the case of using the plant extract alone, it often shows slight skin improvement efficacy.

Under these backgrounds, the present inventors have studied synergistic effects and cooperative action when using two or more of the extracts from Cheongho, Yeongpung and green tea as a result of research to solve the minor skin improvement efficacy effect of using these plant extracts alone. It was confirmed that the improvement and elasticity promoting effect is excellent, and the skin improvement effect is more excellent than the use of the extracts alone, and the present invention has been completed.

An object of the present invention is to provide a composition for improving a skin condition containing two or more mixed extracts selected from the group consisting of Cheongho, Yeungpung and green tea, or a fraction thereof, and a method for improving the skin condition using the same.

Specifically, one object of the present invention is to improve skin texture, improve skin elasticity, prevent skin wrinkles or improve skin, containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Cypress, and Green Tea as active ingredients. It is to provide a cosmetic composition, quasi-drug composition or food composition for preventing or improving aging and improving skin inflammation.

Another object of the present invention is to provide a pharmaceutical composition for the prevention or treatment of skin diseases containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green tea as an active ingredient.

Another object of the present invention is to provide a method of improving skin conditions or preventing or treating skin diseases using the compositions.

In order to solve the above problems, as an embodiment of the present invention, as a cosmetic composition for improving skin condition containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Yeongpung and green tea as an active ingredient, the skin The improvement of the condition provides a cosmetic composition that is one or more selected from the group consisting of skin texture improvement, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, and skin inflammation improvement.

In another embodiment of the present invention, the composition may include one or more selected from the group consisting of Centella and green tea, and a mixed extract of Cheongho or a fraction thereof.

In another embodiment of the present invention, the composition may be one containing a mixed extract of Cheongho, Yeungpung and green tea or a fraction thereof.

Cheongho ( Artemisiae Annuae Herba) of the present invention refers to the above-ground portion of the dicotyledonous campanula family Artemisia annua Linne or Artemisia apiacea Hance. The wormwood mugwort is also called wormwood, small leaf wormwood, ultra high, hyanggo, dog wormwood, wormwood, etc. and inhabit roadsides, glade, and riversides, and are distributed in Korea, Japan, Taiwan, Mongolia, and Siberia. The wormwood mugwort is the ground part, the stem is cylindrical, branched from the top, and is 30 ~ 80 cm long and 2 ~ 6 mm in diameter. The outer surface is yellow-green to yellow-brown, and has vertical ridges. The vagina is hard and well cut, the leaves are opposite, dark green to greenish brown, and brittle. The whole thing is deep in the right idol three times when opened, and the lobe and small lobe are round or long oval, and the bristles are covered on both sides. Dogwormwood has a distinctive scent and slightly bitter taste. Kaesan wormwood is a stem with a flower stalk with few leaves in the upper part and is 60 ~ 90 cm long. The outer surface of the stem is yellowish brown, the vagina is hard, and the curved surface is fibrous. The leaves are greenish brown, finely wrinkled, curled, brittle, and yellowish green. When the cross section of the dog's wormwood is viewed under a microscope, the epidermal cells at the corners of the stem are prominent outward, and the inside is composed of olfactory tissue. The vascular bundle is concentrated in the corner area, and the fiber bundle is arranged in the shape of a half moon around the top. The dog wormwood has a peculiar smell and a mild taste.

A substance called artemisin, which can only kill and kill cancer cells contained in Cheongho, is known to be a sedative component that is 1200 times stronger than conventional anticancer drugs. In addition, Cheongho is known to have antipyretic analgesic, immune enhancement, liver function protection, and anti-malaria action.

Centella asiatica of the present invention is a perennial plant belonging to the mountain family. Cypress is also called gotucola, tiger grass, clam grass or horseshoe grass, and is often native to the mountains and fields of the southern islands of the Korean peninsula. The main stem extends to the side, and there are two degenerated scale-like leaves near the root node. The leaf is dense at the node, elongated, 2 ~ 5cm in diameter, the surface is shiny, there are dull teeth on the edges, and the petiole is 4 ~ 20cm long. The flowers are reddish-purple, and 3 ~ 4 flowers on the leaf axil, in mountain-shaped inflorescence, are shaped like a head. The pistil is two-pronged, and the fruit is a round shape of the subfamily. Centella asiatica, a component of Madecassol, is used as a soothing ingredient to soothe weakened skin due to irritation and to strengthen its own vitality.

Green tea of the present invention refers to the leaves of the dried tea tree without being fermented and roasted as it is. Tea tree ( Camellia sinensis ) is a perennial evergreen tree belonging to the Camellia family, and is widely cultivated in subtropical regions such as Africa and Russia, mainly in Korea and Asia. The main components of green tea are catechin, caffeine, protein, free amino acid, starch, fiber, pectin, chlorophyll, and flovonol derivatives. Green tea is known for its anti-cancer effect, prevention of hypertension and arteriosclerosis, anti-diabetes effect, and anti-obesity effect. In addition, green tea contains a large amount of catechins, which prevents skin damage caused by ultraviolet rays, cares for pores and keeps them elastic, and green tea is a general-purpose soothing ingredient that is good to rinse with water.

As used in the present invention, the term "extract" is an extract obtained by the extraction treatment of plants, a dilution or concentrate of the extract, a dried product obtained by drying the extract, a crude product or a purified product of the extract, or a mixture thereof, etc. It includes the extract itself and extracts of all formulations that can be formed using the extract. The extract of the present invention may preferably be prepared and used in dry powder form after extraction.

The extract of the present invention can be extracted from natural, hybrid or varietal plants of the respective plants, and can also be extracted from plant tissue cultures.

In the present invention, the type of the extraction solvent used to extract the plant is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, glycerin, alcohol, 1,3-butylene glycol, and one or more solvents selected from the group consisting of ethyl acetate, and the like, and when using alcohol as a solvent More preferably, alcohols of C ₁ to C ₄ can be used. The extract of the present invention may preferably be water, glycerin or ethanol extract.

In the extract of the present invention, the method for extracting the plant is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method may include vacuum low temperature extraction, room temperature extraction, hot water extraction, cold immersion extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, etc., which may be performed alone or in combination of two or more methods. Can be performed.

The extract of the present invention may be more preferably extracted by a vacuum low temperature extraction method. Vacuum low temperature extraction is a method of extraction under low temperature conditions using an extraction solvent under vacuum.

In the case of extracting by the vacuum low temperature extraction method in the present invention, it is preferable to repeatedly extract one to five times, and it is more preferable to repeatedly extract three times, but is not limited thereto. The extraction solvent may be added 0.1 times to 10 times based on the weight of the dried plant, preferably 0.3 times to 5 times. The extraction temperature may be preferably 30 ° C to 70 ° C, more preferably 40 ° C to 60 ° C, most preferably 60 ° C, and the extraction time is preferably 12 hours to 48 hours, but is not limited thereto. . When extracting by the vacuum low temperature extraction method, there is an advantage that can improve the extraction efficiency without destroying or loss of the active ingredient, furthermore, in the case of preparing the mixed extract by the vacuum low temperature extraction method, the skin condition is improved by the collaboration between the active ingredients , There is an advantage that the skin disease treatment efficacy becomes more excellent.

In the method for producing the extract of the plant of the present invention, the vacuum concentration is preferably a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, drying is preferably carried out under reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

According to a specific embodiment of the present invention, after washing chungho, bottled green tea and green tea with water and drying in the shade, crushing the dried cheonho, bottled green tea and green tea into an extraction container, and vacuuming at a low temperature of 60 ° C. as an extraction solvent. Extracted repeatedly to obtain an extract. The obtained extract was filtered by removing solids using filter paper or the like, and then the extract was concentrated under reduced pressure to prepare an extract.

The term "fraction" as used in the present invention means a result obtained by performing a fraction to separate a specific component or a specific component group from a mixture containing various various constituent components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. As a non-limiting example of the fractionation method, a method of obtaining a fraction from the extract by treating a predetermined solvent with an extract obtained by extracting two or more kinds selected from the group consisting of Cheongho, Yeolpung and Green Tea may be mentioned.

In the present invention, the type of the solvent used to obtain the fraction is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fractional solvent include polar solvents such as water, glycerin and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohol is used in the fractional solvent, alcohols of C ₁ to C ₄ may be preferably used.

The two or more mixed extracts selected from the group consisting of the cheongho, bottled grass and green tea of the present invention, respectively, after extracting the cheongho, bottled grass and green tea, respectively, or by mixing two or more extracts, or the cheongho, bottled grass and green tea It can be prepared by extracting together two or more selected from the group consisting of.

The two or more mixed extracts selected from the group consisting of the Cheongho, Byeongpul and green tea of the present invention have a superior synergistic effect in anticancer efficacy than when using the respective extracts of Cheongho, Byeongpul or Green Tea alone. Shows. In particular, two or more kinds of mixed extracts selected from the group consisting of Cheongho, Cypress, and green tea are excellent synergy for improving skin texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation. It shows an effect, and it shows an improvement in the skin condition to a considerable extent, or the prevention, improvement or treatment effect of skin diseases compared to the case where each of them is used alone.

The weight ratio of two or more selected from the group consisting of the extract of Cheongho contained in the composition of the present invention or a fraction thereof, an extract of Centella or a fraction thereof, and an extract of green tea or a fraction thereof is not particularly limited. In the case of a mixed extract, it is preferably about 1: 1, and in the case of three types of mixed extract, it may be preferably contained in a weight ratio of about 1: 1: 1. When contained in the above range, it is possible to effectively improve the skin condition and has the advantage of effectively preventing, improving or treating skin diseases.

The two or more mixed extracts or fractions thereof selected from the group consisting of the Cheongho, Yeongpung and green tea of the present invention are sole extracts of each of the plants by synergistic effect or cooperative action between the extracts and / or the active ingredients in the extract. It shows a better skin condition improvement efficacy compared to this, which was first identified in the present invention.

Specifically, two or more mixed extracts or fractions thereof selected from the group consisting of the Cheongho, Yeongpung and green tea of the present invention exhibit an effect of improving skin texture and improving skin elasticity. In addition, two or more mixed extracts or fractions thereof selected from the group consisting of the Cheongho, Yeongpung and green tea of the present invention exhibit excellent anti-inflammatory efficacy. Particularly, two or more mixed extracts or fractions thereof selected from the group consisting of the Cheongho, Yeungpung and green tea of the present invention exhibit better nitric oxide (NO) production inhibitory effect than the single extract of each plant.

According to an embodiment of the present invention, the essence containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Yeongpung and Green Tea is targeted at 30 women aged 35 to 50 years old on the face area for 3 months When applied, it was confirmed that it shows an effect of improving skin texture and improving skin elasticity (Experimental Example 1), and it was found through these results that skin wrinkle prevention or improvement, skin aging prevention or improvement effect can also be exhibited.

In addition, according to an embodiment of the present invention, when two or more mixed extracts selected from the group consisting of Cheongho, Yeungpung and Green Tea or fractions thereof are treated in fibroblasts, collagen synthesis is promoted higher than that of the extract alone. The effect was confirmed (Experimental Example 2), and through these results, it was found that the elasticity enhancement, skin aging prevention or improvement, and skin wrinkle improvement effect can be exhibited.

In addition, according to an embodiment of the present invention, when two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Yeolpung and Green Tea are treated in Raw 264.7 cells, nitrogen monoxide is higher than the extracts of the extracts alone. (NO) It was confirmed that it can exhibit a more excellent anti-inflammatory effect by showing a production inhibitory effect (Experimental Example 4), and through these results, it was found that it can also exhibit skin anti-aging or improvement and skin inflammation improvement effects.

In addition, the composition of the present invention does not exhibit cytotoxicity in a concentration range sufficiently exhibiting the effect of improving the skin condition, and is derived from a natural product, and has the advantage that it can be stably used without seriously irritating or causing harmful effects in the body. Yes (Experimental Example 3).

The term used in the present invention, "improve skin condition" may mean one or more selected from the group consisting of skin texture improvement, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, and skin inflammation improvement. .

As used herein, the term "improvement" means any action that at least reduces the severity of a condition or a parameter associated with treatment, such as at least a symptom.

The cosmetic composition according to the present invention is a solution, ointment for external use, cream, foam, nutrition lotion, soft lotion, perfume, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil , Suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, can be prepared in a formulation selected from the group consisting of patch and spray, It is not limited thereto.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and for example, oil, water, surfactant, moisturizer, lower alcohol, and thickener as common ingredients , Chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended, but is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention may vary depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. as carrier components This may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and in the case of a spray, additionally, chlorofluorohydro Propellants such as carbon, propane / butane or dimethyl ether, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, glycerin, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo Eight, propylene glycol, 1,3-butylglycol oil and the like can be used, in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or sorbitan Fatty acid esters may be used, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, liquid diluents such as water, glycerin, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, and the like, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, alkali metal salt of fatty acid, fatty acid hemiester salt, fatty acid protein hydrolyzate, isethionate, lanolin derivative, aliphatic alcohol, vegetable oil, glycerol, sugar, etc. are used as carrier components. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

According to another embodiment of the present invention, as a quasi-drug composition containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Yeungpung and green tea as an active ingredient, the improvement of the skin condition Provides a quasi-drug composition that is one or more selected from the group consisting of improving texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation.

The term "quasi-drug" as used in the present invention refers to articles with less action than pharmaceuticals among articles used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals, for example According to the report, quasi-drug products exclude products used for pharmaceutical use, and include products used to treat or prevent disease in humans and animals, and products that have little or no direct action on the human body.

The quasi-drug composition of the present invention may be prepared from a group consisting of body cleanser, foam, soap, mask, ointment, cream, lotion, essence and spray, but is not limited thereto.

In the quasi-drug composition of the present invention, a description of two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green Tea, skin texture improvement, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement And the description of skin inflammation improvement is the same as described above with respect to the cosmetic composition.

According to another embodiment of the present invention, as a food composition for improving the skin condition containing as an active ingredient two or more mixed extracts selected from the group consisting of Cheongho, Centella and Green Tea, the improvement of the skin condition It provides a food composition, which is at least one selected from the group consisting of improving texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation.

The food composition of the present invention is not particularly limited, and includes a health functional food composition.

When the health functional food composition of the present invention is used as a food additive, the composition may be added as it is or used with other foods or food ingredients, and may be suitably used according to a conventional method.

The type of the food is not particularly limited, and includes all foods in the ordinary sense. Non-limiting examples of foods to which the above substances can be added are meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other dairy products including noodles, gums, ice cream, various soups, beverages, tea , Drinks, alcoholic beverages and vitamin complexes.

When the health functional food composition of the present invention is a beverage composition, it may contain various flavoring agents or natural carbohydrates, etc., as additional components, like a conventional beverage. Non-limiting examples of the natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Natural sweeteners such as dextrin and cyclodextrin; And synthetic sweeteners such as saccharin and aspartame. The proportion of the additional component added can be appropriately determined by the choice of those skilled in the art.

In addition to the above, the health functional food composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin , Alcohol, carbonic acid used in carbonated beverages, and the like. In addition, the health functional food composition of the present invention may contain flesh for the preparation of natural fruit juices, fruit drinks or vegetable drinks. These ingredients may be used independently or in combination of two or more. The proportions of these additives can also be appropriately selected by those skilled in the art.

In the food composition of the present invention, a description of two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green tea, improving skin texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging And the description of skin inflammation improvement is the same as described above with respect to the cosmetic composition.

In the cosmetic composition, quasi-drug composition or food composition of the present invention, two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green Tea are preferably in dry weight, 0.01% to 20.0% by weight of the total composition It may be contained, and more preferably, it may be contained in 0.1% to 10% by weight. Within this range, there is an advantage of improving skin condition without cytotoxicity.

According to another embodiment of the present invention, there is provided a pharmaceutical composition for the prevention or treatment of skin disorders containing as an active ingredient two or more mixed extracts selected from the group consisting of Cheongho, Centella and Green Tea as an active ingredient.

The skin disease includes at least one selected from the group consisting of skin wrinkles, skin aging and skin inflammatory diseases.

In the pharmaceutical composition of the present invention, a description of two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green Tea, and descriptions of skin wrinkles, skin aging, and skin inflammation are related to the cosmetic composition. It is the same as described in the.

The skin inflammatory disease of the present invention means any disease caused by immune stimulation. Non-limiting examples of the skin inflammatory disease include atopic dermatitis, eczema, seborrheic dermatitis, psoriasis, acne.

In the pharmaceutical composition of the present invention, the effective content of two or more kinds of mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green Tea is not particularly limited, and is 0.00001 wt% to 99.99 wt% based on the total weight of the pharmaceutical composition It may be included as or may be included in the total content.

The pharmaceutical composition of the present invention, in addition to containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Cypress, and green tea as an active ingredient, may further include a pharmaceutically acceptable carrier.

In the present invention, the term "pharmaceutically acceptable" means that it can be conventionally used in the pharmaceutical field, without stimulating the organism when administered, without inhibiting the biological activity and properties of the compound to be administered.

The pharmaceutical composition of the present invention is formulated with the carrier, it can be used as food, pharmaceuticals, feed additives, drinking water additives and the like.

In the present invention, the type of the carrier is not particularly limited, and any carrier conventionally used in the art may be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, ethanol, and the like. Can be. These may be used alone or in combination of two or more.

In addition, the pharmaceutical composition of the present invention can be used by adding other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostatic agents, if necessary, fillers, extenders, wetting agents, disintegrants, dispersants, surfactants , Binders or lubricants may be added and used.

The pharmaceutical composition of the present invention may be formulated and used in various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of the formulation for oral administration include troches, lozenges, tablets, aqueous suspensions, oily suspensions, preparation powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs, etc. Can be heard.

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin; Excipients such as Dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax can be used, and sweeteners, fragrances, syrups, etc. can also be used. Can be.

Furthermore, in the case of capsules, in addition to the above-mentioned substances, a liquid carrier such as fatty oil may be additionally used.

Non-limiting examples of the parenteral preparation include injection solutions, suppositories, respiratory inhalation powders, spray aerosols, oral sprays, mouthwashes, toothpastes, ointments, powders for application, oils, creams, and the like. have.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, external preparations, etc. can be used, and the non-aqueous solvents, suspensions are propylene glycol, polyethylene Vegetable oils such as glycol and olive oil, and injectable esters such as ethyl oleate may be used.

In addition, more specifically, when the pharmaceutical composition of the present invention is formulated into an injection solution, the composition of the present invention is mixed with water with a stabilizer or a buffer to prepare a solution or suspension, and it is used as an ampoule or vial. It can be formulated for unit administration. In addition, when the pharmaceutical composition of the present invention is formulated as an aerosol agent, a propellant or the like may be combined with an additive so that the dispersed dispersion or wet powder is dispersed.

In addition, when formulating the pharmaceutical composition of the present invention with ointment, cream, etc., animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Etc. can be formulated as a carrier.

The pharmaceutical composition of the present invention may preferably be formulated into a formulation for parenteral administration, more preferably consisting of a gel, patch, spray, ointment, warning agent, lotion agent, linement agent, pasta agent and cataplasma agent It may have a formulation selected from the group.

The pharmaceutically effective amount and effective dose of the pharmaceutical composition of the present invention may be varied by a method of formulating the pharmaceutical composition, a method of administration, an administration time and / or a route of administration, and a reaction to be achieved by administration of the pharmaceutical composition. The type and extent of the target, the type of subject to be administered, the age, weight, general health condition, the severity or severity of the disease, sex, diet, excretion, drugs used simultaneously or simultaneously with the subject, or other components of the composition. It can be varied according to various factors including similar factors well known in the pharmaceutical field, and those skilled in the art can easily determine and prescribe an effective dosage for the desired treatment. Administration of the pharmaceutical composition of the present invention may be administered once a day, it may be divided into several times. Therefore, the above dosage does not limit the scope of the present invention in any way.

The preferred dosage of the pharmaceutical composition of the present invention may be 1 mg / kg to 1, OOO mg / kg per day.

The route of administration and the mode of administration of the pharmaceutical composition of the present invention may be independent of each other, and are not particularly limited in the manner, and as long as the pharmaceutical composition can reach the target site of interest in any route and mode of administration. Can follow. The pharmaceutical composition may be administered by oral administration or parenteral administration.

As the method of parenteral administration, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration, or subcutaneous administration may be used, and the composition may be applied to a diseased site, sprayed, or inhaled. May be, but not limited to.

The pharmaceutical composition of the present invention may preferably be administered parenterally, and more preferably, it may be administered in a manner of topically applying to an area in need of preventing, improving or treating skin conditions or improving skin condition. have.

As used in the present invention, the term "prevention" refers to all actions to suppress or delay the development of skin diseases including skin wrinkles, skin aging and skin inflammatory diseases by administering the pharmaceutical composition of the present invention to an individual. .

As used in the present invention, the term "treatment" refers to all actions to improve or benefit the symptoms of skin wrinkles, skin aging and skin inflammatory diseases by administering the pharmaceutical composition of the present invention to an individual.

According to another embodiment of the present invention, comprising the administration of a cosmetic composition, quasi-drug composition or food composition containing as an active ingredient two or more mixed extracts selected from the group consisting of Cheongho, Centella and Green Tea as an active ingredient. It provides a method for improving the skin condition.

The term "individual" in the present invention means all animals, including mammals, including rats, livestock, humans, and the like.

In the present invention, the cosmetic composition, quasi-drug composition or food composition containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Centella and Green tea is the same as described above, meaning of improving skin condition It is also the same as described above.

In the present invention, the description of the dosage, route of administration, administration method, and the like of the composition is replaced with the contents described in connection with the pharmaceutical composition of the present invention.

According to another embodiment of the present invention, comprising the administration of a pharmaceutical composition containing as an active ingredient two or more mixed extracts selected from the group consisting of Cheongho, Centella and Green Tea as an active ingredient, prevention of skin diseases Or provide a method of treatment.

In the present invention, the pharmaceutical composition containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Yeolpung, and green tea as the active ingredient is the same as described above, and the meaning of the individual, skin disease, prevention, treatment is also It is the same as described above.

In the present invention, a description of the dosage, route of administration, and mode of administration of the pharmaceutical composition is the same as described in connection with the pharmaceutical composition of the present invention.

The composition containing two or more mixed extracts or fractions thereof selected from the group consisting of Cheongho, Yeungpung and green tea of the present invention as an active ingredient may be used as a synergistic effect or cooperative action between extracts and / or active ingredients in the extract. It has excellent effect of improving texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation. Specifically, the composition of the present invention exhibits excellent skin condition improvement effects such as improving skin texture, improving skin elasticity, preventing aging through improving collagen synthesis, and improving wrinkles. In particular, nitrogen monoxide (better than the single extract of each plant) NO) It has an excellent effect of suppressing the inflammatory reaction by showing a production inhibitory effect.

In addition, since the composition of the present invention is a substance derived from natural products, it is non-toxic and does not cause side effects even when applied to the human body, and thus has high utilization as a cosmetic, quasi-drug, food and / or pharmaceutical composition.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Preparation Example 1: Preparation of plant extracts

In order to solve the slight effect of skin improvement in the case of using the plant extracts alone, in order to prepare the extracts extracted together with not only the flavonoids of each plant but also the components capable of cooperative action, the efficacy components are destroyed by a vacuum low temperature method. Without.

Specifically, cheonho, bottled green tea and green tea were washed with water and dried in the shade, and then the dried cheonho, bottled green tea and green tea were crushed and put into an extraction container, followed by repeated extraction under vacuum at a low temperature of 60 ° C with glycerin to extract each. Was obtained. The obtained extract was filtered by removing solids using filter paper or the like, and then the extract was concentrated under reduced pressure to prepare an extract.

Examples 1-6 and Comparative Examples: Preparation of essence containing mixed extract of the present invention

A cosmetic composition of an essence formulation was prepared as shown in Table 1 below (unit: wt%). At this time, in this example, after preparing each extract of Cheongho, Yeungpung and green tea, they were mixed to prepare a mixed extract.

Raw material name Comparative example Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Cetearyl alcohol 2 2 2 2 2 2 2 Cetearyl glucoside One One One One One One One Jojoba seed oil 5 5 5 5 5 5 5 Silica 0.5 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 10 10 10 10 10 10 10 Cheongho Extract - 2.5 2.5 2.5 - - - Centella extract - - 2.5 - 2.5 - 2.5 Green Tea Extract - - - 2.5 - 2.5 2.5 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100

Experimental Example 1: Measurement of skin improvement effect

For the products of Comparative Examples and Examples 1 to 6, skin improvement effects were performed on healthy 35- to 50-year-old women as follows.

Essences of Examples 1 to 6 containing 30% by weight of a mixture of two extracts of Cheongho, Byeongpul and Green Tea in the right eye area, and 30 females aged 35 to 50 years 1 It was applied twice a day for 3 months.

After 3 months, the degree of improvement of the skin was evaluated through a subject's questionnaire and image analysis of the skin. The subject's questionnaire was judged in three stages of no improvement, slight improvement, and significant improvement compared to before using skin texture improvement and elasticity enhancement, and the results are shown in Table 2 (unit: persons).

sample No improvement Slight improvement Significant improvement Comparative example 8 2 0 Example 1 0 4 5 Example 2 0 4 5 Example 3 0 5 4 Example 4 One 5 4 Example 5 One 4 5 Example 6 2 4 4

As can be seen from Table 2, when Examples 1 to 6 according to the present invention were used, it can be seen that the effect of improving the skin condition including the skin texture improvement and the elasticity enhancing effect is excellent.

Experimental Example 2: Measurement of collagen synthesis promoting effect

For the mixed extract prepared in Preparation Example 1, the effect of enhancing collagen synthesis was measured using cells cultured as follows.

First, human fibroblast cells were cultured in a 24-well plate (24-well plate, corning company) for 24 hours, and then L-2,3-3H proline was reacted for 24 hours. At this time, as a test group, 1% by weight of the mixed extracts of the two types of Cheongho, Yeungpung, and Green Tea were each added, and nothing was treated as a control.

After reacting the L-2,3-3H proline, the amount of new collagen secreted by fibroblasts in the cell medium was measured and compared using a collagen degrading enzyme and a radioisotope activity meter. Serum 1% was used as a control, and serum-free medium was used as the experimental group.

Type of sample Collagen synthesis enhancement effect (%) Control 260 Cheongho + Bottleweed 190 Cheongho + Green Tea 187 Bottle grass + green tea 172

As can be seen from the results in Table 3, it was found that the mixed extracts of two kinds of Cheongho, Yeungpung, and green tea had an excellent effect of enhancing the synthesis of collagen at the cellular level. Or it was found that the improvement and the effect of improving skin wrinkles can be exhibited.

Experimental Example 3: Cytotoxicity Test

In order to verify the primary safety as a raw material used in cosmetics, V79-4 cells (Chinese hamster, continuous cell line of lung tissue fibroblasts) were mixed with respect to the mixed extract of two kinds of Cheongho, Perilla and Green tea prepared in Preparation Example 1 above. Cultured to MTT test [Reference: Mossman T. (1983). Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55-63. Table 4 shows the results of the cytotoxicity test.

sample ^a IC ₅₀ (%, w / v) Sodium lauryl sulfate (SLS) 0.005 Squalane 0.05 Cheongho + Bottleweed One Cheongho + Green Tea One Bottle grass + green tea One

Note) ^a IC ₅₀ : Inhibitory Concentration 50, 50% cell killing concentration

As shown in Table 4, the IC ₅₀ value of the two mixed extracts of Cheongho, Yeungpung, and Green Tea was 1.0, and the cytotoxicity was lowered by 2000 times or more compared to sodium lauryl sulfate, a synthetic surfactant used in cosmetics. In addition, it can be seen that, even when compared to squalane, which is a useful material having excellent safety, cytotoxicity is lower than 200 times, safety is very excellent.

Experimental Example 4: Anti-inflammatory effect test (NO Assay)

Raw 264.7 cells were used to evaluate the inhibitory effects of NO generation on the same concentrations of Cheongho, Yeongpung, and Green tea extracts and their two mixed extracts and three mixed extracts.

The sample was diluted to a concentration of 1% by weight, pre-treated for 30 minutes, treated with 1 mg / ml LPS, incubated for 24 hours, and then the NO production inhibitory ability was evaluated using a NO quantitative kit as follows.

NO generation inhibitory ability (%) = {1- (NO production amount when sample is added / NO production amount when sample is not added)} x100

Positive control: 10mg / ml L-NMMA1 (NOS inhibitor)

The results are shown in Table 5 below.

1% sample Positive control Cheongho Bottleweed green tea Cheongho
+ Bottleweed
(1: 1) Cheongho
+ Green tea
(1: 1) Bottleweed
+ Green tea
(1: 1) 3 types
mixture
(1: 1: 1) NO increase inhibition (%) 42 20 18 16 42 41 35 48

As shown in Table 5, compared to each single extract, in the case of two or more mixed extracts treated with the same concentration, it was confirmed that the ability to inhibit NO generation is twice as high, and the mixed extract according to the present invention was used for each single extract. It can be seen that it has a better cooperative action than the extract, and it can be seen that it is very effective in preventing or improving skin aging and improving skin inflammation.

Claims (7)

A cosmetic composition for improving skin condition comprising one or more selected from the group consisting of coriander and green tea and a mixed extract of Cheongho or a fraction thereof as an active ingredient, wherein the improvement of the skin condition is improvement of skin elasticity or prevention or improvement of skin wrinkles Will, cosmetic composition.
The cosmetic composition according to claim 1, wherein the composition contains a mixed extract of Cheongho, Centella and green tea or a fraction thereof.
The cosmetic composition of claim 1, wherein the improvement of the skin condition is performed by inhibiting nitrogen monoxide production.
As a quasi-drug composition for improving skin condition, comprising one or more selected from the group consisting of coriander and green tea and a mixed extract of Cheongho or a fraction thereof as an active ingredient, wherein the improvement of the skin condition is improvement of skin elasticity or prevention or improvement of skin wrinkles Will, quasi-drug composition.
The quasi-drug composition according to claim 4, wherein the composition contains a mixed extract of Cheongho, Cypress, and green tea or a fraction thereof.
A pharmaceutical composition for the prevention or treatment of skin diseases containing one or more selected from the group consisting of coriander and green tea and a mixed extract of Cheongho or a fraction thereof as an active ingredient, wherein the skin disease is skin wrinkles.
The pharmaceutical composition of claim 6, wherein the composition contains a mixed extract of Cheongho, Cypress, and green tea or a fraction thereof.