KR20220068595A - Functional bath cleanser composition comprising plant powder - Google Patents

Abstract

According to the present invention, disclosed are a food composition and a cosmetic composition, particularly a bath composition having functions such as anti-oxidation, whitening, wrinkle improvement, anti-inflammation, injury treatment, moisturizing and the like by including plant components such as 1 to 40% by weight of green tea, 1 to 20% by weight of centella asiatica, 1 to 30% by weight of mugwort, 1 to 10% by weight of ginger and the like.

Description

Functional bath cleaner composition comprising plant powder

The present invention relates to a composition comprising a plant powder or plant extract. Specifically, it relates to a cosmetic composition or a food composition comprising a plant powder or a plant extract as a main component, and more particularly, to a functional bath composition.

Modern people are easily exposed to skin damage such as skin dryness and keratin formation due to various causes such as exposure to environmental pollutants, stress, frequent washing with color makeup, and natural skin aging due to increasing age. Therefore, efforts to protect and improve the skin are required.

When inflammation is induced in cells by various stresses, NO (Nitric Oxide), one of the active species, is generated. It is known to cause damage to the skin and cells and induce aging. Neutrophils, which are closely related to inflammation, migrate to the dermal layer and secrete elastase when the skin becomes inflamed. The secreted elastase causes wrinkles by decomposing elastin, which is involved in the elasticity of the skin. In addition, when inflammation occurs in the skin, hyperpigmentation occurs through an increase in tyrosinase activity by inflammatory mediators. Hyaluronidase, which is involved in the skin moisturizing function, is an enzyme that hydrolyzes hyaluronic acid, and the activity of hyaluronidase may cause skin dryness.

A bath agent refers to a drug that can not only recover from fatigue or protect the skin, but also treat diseases and relieve symptoms when taking a bath after mixing active substances in an appropriate concentration. People have used natural hot springs for wound healing and health promotion since ancient times, and various natural plants have been used for skin health purposes. Recently, as baths such as half-body baths and aroma baths are known to be beneficial to health, bath therapy using bath products containing various natural plant ingredients is attracting great attention. However, although there are various bath products containing mainly chemical components, bath products manufactured with plant components are not diverse. Therefore, there is a need to develop a bath composition that is effective in antioxidant, anti-inflammatory, wound healing, moisturizing, etc.

Laid-Open Patent Publication No. 10-2017-0009411

Seungheon Han et al., KOREAN JOURNAL OF ORIENTAL MEDICINE 16(3), 107-113(2010) Jeong Jeong, et al., Korean J. Oriental Physiology & Pathology 24(5), 837-842(2010)

In order to solve the above problems, an object of the present invention is to provide a bath composition that provides whitening, anti-wrinkle and anti-inflammatory effects through a blending ratio of green tea, centella asiatica, mugwort, and ginger powder.

In order to achieve the above object, the present invention provides a composition containing 1 to 40% by weight of green tea, 1 to 20% by weight of Centella asiatica, 1 to 30% by weight of mugwort, and 1 to 10% by weight of ginger as active ingredients.

In addition, the present invention provides a cosmetic composition or food composition for wrinkle improvement and anti-inflammatory effect comprising green tea, centella asiatica, mugwort, and ginger extract as an active ingredient.

The bath composition comprising green tea, centella asiatica, mugwort, and ginger powder according to the present invention as an active ingredient inhibits DPPH radical scavenging activity; inhibition of elastase enzyme activity; inhibition of tyrosinase activity; Excellent wrinkle improvement, anti-inflammatory activity, wound prevention, improvement or treatment effect, moisturizing effect, etc. were confirmed through cell viability measurement and nitric oxide (NO) production measurement. Prevention, improvement or treatment of wrinkles and skin inflammatory diseases can be used to

1 shows the evaluation results of DPPH scavenging ability of a composition according to the present invention.
2 shows the evaluation results of tyrosinase activity inhibition of the composition according to the present invention.
3 shows the evaluation results of the elastase activity inhibition of the composition according to the present invention.
4 shows the results of the evaluation of inhibition of inflammation of the composition according to the present invention.
5 shows the evaluation results of the prevention or improvement effect of the composition according to the present invention.
6 shows the moisturizing effect results of the composition according to the present invention.

Hereinafter, the present invention will be described in more detail as one embodiment as follows.

The present invention provides a composition comprising green tea powder, centella asiatica powder, mugwort powder, and ginger powder as active ingredients.

According to the present invention, the composition comprising green tea powder, centella asiatica powder, mugwort powder, ginger powder, or the composition comprising green tea extract, centella asiatica extract, mugwort extract, and ginger extract, has problems related to the inappropriate reaction of the bathing agent to the skin and skin diseases. It is useful as a cosmetic composition or a food composition that essentially solves or helps.

According to a preferred embodiment of the present invention, the composition can be used as a bath composition contained as an active ingredient for wrinkle improvement and anti-inflammatory

According to a preferred embodiment of the present invention, the cosmetic composition or the food composition may be in a mixed form with additives or excipients or other ingredients that are typically used in cosmetic compositions and food compositions.

The cosmetic composition according to the present invention contains 1 to 40% by weight of green tea (or green tea extract), 1 to 20% by weight of Centella asiatica (or Centella asiatica extract), 1 to 30% by weight of mugwort (or mugwort extract), ginger (or ginger extract) 1 ~10% by weight, preferably 30-40% by weight of green tea, 20-30% by weight of Centella asiatica, 20-30% by weight of mugwort, 10-20% by weight of ginger.

In an embodiment of the present invention, the cosmetic composition comprises 40% by weight of green tea (or green tea extract), 20% by weight of Centella asiatica (or Centella asiatica extract), 30% by weight of mugwort (or mugwort extract), 10% by weight of ginger (or ginger extract) may contain

In the case of the food composition according to the present invention, 1 to 40% by weight of green tea (or green tea extract), 1 to 20% by weight of Centella asiatica (or Centella asiatica extract), 1 to 30% by weight of mugwort (or mugwort extract), ginger (or ginger extract) 1 to 10% by weight, preferably 30 to 40% by weight of green tea, 20 to 30% by weight of Centella asiatica, 20 to 30% by weight of mugwort, and 10 to 20% by weight of ginger.

In an embodiment of the present invention, the food composition comprises 40% by weight of green tea (or green tea extract), 20% by weight of Centella asiatica (or Centella asiatica extract), 30% by weight of mugwort (or mugwort extract), 10% by weight of ginger (or ginger extract) may contain

If the plant components such as green tea (or green tea extract), centella asiatica (or centella asiatica extract), mugwort (or mugwort extract), ginger (or ginger extract) contained in the composition of the present invention are out of the above-mentioned mixing ratio, toxicity is induced or The antioxidant effect, the whitening effect, the wrinkle improvement effect, the anti-inflammatory effect, the prevention, improvement or treatment effect of skin wounds, and the moisturizing effect may be reduced.

The bath composition containing green tea extract, centella asiatica extract, mugwort extract, and ginger extract according to the present invention can be prepared by extracting green tea, centella asiatica, mugwort, and ginger with a polar solvent to prepare an extract.

According to a preferred embodiment of the present invention, green tea, centella asiatica, mugwort, and ginger powder may be extracted using water, an alcohol having 1 to 6 carbon atoms, or an aqueous alcohol solution to prepare a green tea, centella asiatica, mugwort, and ginger complex extract.

According to a preferred embodiment of the present invention, green tea extract, centella asiatica extract, mugwort extract, and ginger extract are most preferably extracts obtained by extracting green tea, centella asiatica, mugwort, and ginger pulverized product with a polar solvent. have.

The extraction is preferably performed at 40-100° C., more preferably at 30-40° C. If the temperature is too low, the efficacy may appear weak, the extraction time will be long, and if the extraction is at too high temperature, there may be denaturation of the components of green tea extract, centella asiatica extract, mugwort extract, and ginger extract.

The wrinkle-improving and anti-inflammatory bath composition of the present invention can be used to prepare a food composition for preventing or recovering skin damage and a cosmetic composition for preventing and recovering skin damage.

In the present invention, green tea extract, centella asiatica extract, mugwort extract, and ginger extract can be mixed with various common food ingredients to prepare food.

The cosmetic composition containing green tea extract, centella asiatica extract, mugwort extract, and ginger extract of the present invention can be used in a variety of basic products such as lotions, essences, creams, packs, foundations, makeup bases, color products, hair products, bath products, etc. It can be prepared in a dosage form. Specifically, it can be applied in various properties such as liquid, cream, paste, and solid, and can be applied to a conventional cosmetic preparation method.

The anti-wrinkle effect was confirmed by inhibiting elastase through the mixing ratio of green tea, centella asiatica, mugwort, and ginger powder according to the present invention, and the whitening effect was confirmed by inhibiting the activity of tyrosinase involved in melanin formation, hyaluronic acid It was confirmed that there was a moisturizing effect by inhibiting the activity of Ronidase. In addition, the anti-inflammatory effect was confirmed in macrophage cells, and it was confirmed that wound healing was excellent in keratinocytes.

Therefore, the green tea extract, centella asiatica extract, mugwort extract, and ginger extract according to the present invention inhibited elastase, tyrosinase, and hyaluronidase activity, and confirmed the anti-inflammatory effect in macrophage cells and wound healing in keratinocytes. Thus, it has the effect of preventing and improving skin diseases.

Hereinafter, specific examples such as Examples, Experimental Examples, etc. will be described to describe the present invention in detail.

However, the embodiments or experimental examples according to the present invention may be modified in various other forms, and the scope of the present invention is not to be construed as being limited to the examples described below.

Example 1: Preparation of a composition according to the present invention and preparation of an extract

Green tea, centella asiatica, mugwort, and ginger were washed several times with a washing machine so that there were no foreign substances using purified water, and then dried using a dryer. Drying was dry enough to break the plants easily. The dried plants were crushed using a crusher, and a composition was prepared by uniformly mixing 40% by weight of green tea, 20% by weight of Centella asiatica, 30% by weight of mugwort, and 10% by weight of ginger. 10 times purified water was added to the mixed plant, adjusted to 40° C., extracted for 4 hours, and filtered. The extract was dissolved in purified water at 100 mg/ml to prepare a stock solution and used in the experiment.

Example 2: Preparation of extract according to the present invention

500 ml of purified water was added to 50 g of green tea, centella asiatica, mugwort, and ginger each or a mixed plant as shown in Table 1, extracted by heating at 40° C. for 4 hours, and filtered. The extract was dissolved in purified water at 100 mg/ml to prepare a stock solution and used in the experiment.

plant mixing ratio Green tea: Centella asiatica: Mugwort: Ginger 4:3:2:1 Green tea: Centella asiatica: Mugwort: Ginger 4:2:3:1 Green tea: Centella asiatica: Mugwort: Ginger 3:3:3:1 Green tea: Centella asiatica: Mugwort: Ginger 4:2:2:2

Experimental Example 1. Evaluation of the antioxidant effect of the composition according to the present invention

In order to examine the antioxidant effect of the composition according to the present invention, a Free Radical Scavenging Activity Test was performed. The free radical scavenging test is a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299~303 (1993)), and the stable free radical DPPH (1,1-Diphenyl-2-picrylhydrazyl radical (Sigma) was used.

First, the plant extract sample obtained according to Example 2 was diluted to an appropriate concentration in methanol in 1 ml of 0.2 mM DPPH solution, mixed with 2 ml, and left at room temperature for 10 minutes, and then absorbance at 517 nm was measured.

On the other hand, the control in the free radical scavenging test was measured in the same way by putting methanol instead of the sample solution, and adding methanol instead of the DPPH solution to obtain each color correction value for the plant extract sample and the control.

As can be seen in FIG. 1 , in the complex extract according to the present invention, excellent effects were observed in the 4:2:3:1, 3:3:3:1, and 4:2:2:2 extracts.

Experimental Example 2 : of the composition according to the invention in vitro Tyrosinase activity inhibition test

Tyrosinase is an enzyme that helps to produce melanin by accelerating the oxidation process of a substance called tyrosine in a living body. In this experimental example, the above-described implementation by applying the method (Pomerantz S.H., J. Biochem., 24:161-168, 1966) for measuring the degree of inhibiting the formation of melanin from the oxidation of tyrosine by inhibiting the function of this enzyme The whitening effect of the plant extract obtained according to Example 2 was evaluated.

In the tyrosinase activity inhibition test, the control group added 0.1 M phosphate buffer (pH 6.8) instead of the plant extract sample and measured in the same manner.

The results were shown in FIG. 2 as Significant results according to students test (p < 0.05), (p < 0.01).

As a result of the experiment, the tyrosinase activity inhibitory effect of the plant extract according to Example 2 was excellent in the complex extract formulated in 4:2:3:1 and 4:2:2:2.

Experimental Example 3 : Elastase activity inhibition test of the composition according to the present invention

An Elastase Inhibition Activity Test was performed as a test for skin wrinkle inhibition and improvement effect by using the plant extract obtained according to Example 2 as a sample.

As a method of measuring the activity of elastase, an enzyme that decomposes elastin, the color change caused by the decomposition of ρ-nitroaniline is measured using N-Succinyl-Ala-Ala-Ala ρ-nitroaniline, which is an elastase substrate. This is a method for measuring elastase activity by measuring absorbance at a wavelength of 410 nm. The buffer solution is pH 8.0, 0.267 M Trizma-HCl (Sigma), the substrate solution is 8.8 mM N-Succinyl-(Ala) ₃ ρ-nitroaniline (Sigma), and the enzyme solution is 10 μg/ml (Sigma) of pig pancreatic elastase. concentration was used. 60 μl of the buffer solution, 20 μl of the substrate solution, and each of the plant extracts obtained according to Examples 1 and 2 above were dissolved in purified water at each concentration to make 100 μl of the sample solution, and then 20 μl of the enzyme solution was added in a water bath at 25° C. After reacting for 15 minutes, the amount of ρ-nitroaniline produced is measured at a wavelength of 405 nm using a microplate reader.

On the other hand, the control group for measuring the elastase activity was measured in the same manner by adding purified water instead of a plant extract, and a case where purified water was added instead of an enzyme solution to obtain color correction values for each was set (References: Lim, Suk- Won et al., The effect of two terpenoids, ursolic acid and oleanolic acid on epidermal permeability barrier and simultaneously on dermal functions, Journal of the Korean Cosmetic Society, 29(2), 205-232, 2003).

The results were shown in FIG. 3 as Significant results according to students test (p < 0.05), (p < 0.01).

As a result of the experiment, the elastase activity inhibitory effect of the plant extract according to Example 2 was excellent in the complex extracts formulated in 4:2:3:1 and 4:2:2:2.

Experimental Example 4: Anti-inflammatory test of the composition according to the present invention

In order to confirm the anti-inflammatory activity of the plant extract obtained in Example 2, the effect on NO (nitric oxide) production, which is known to play an important role in inducing inflammation, was investigated. Although the formation of general NO plays an important role in killing bacteria or removing tumors, NO produced by iNOS in an inflammatory state not only promotes inflammatory reactions such as vascular permeability and edema, but also promotes biosynthesis of inflammatory mediators to reduce inflammation. known to exacerbate it.

RAW264.7 cells were adjusted to 5×10 ⁵ cells/well using DMEM medium supplemented with 10% FBS, and then inoculated in a 24-well plate, sampled and incubated for 30 minutes, LPS (lipopolysaccharide, 1 μg/ml ) was added and cultured for 24 hours. The amount of NO generated was measured using a NO detection kit (iNtRON). The control group for the anti-inflammatory activity test was the experimental group treated with LPS alone.

The results were shown in FIG. 4 as Significant results according to students test (p < 0.05), (p < 0.01).

Looking at the measurement results in FIG. 4 , it was found that the NO reduction effect was excellent in the complex extracts formulated in 4:3:2:1 and 4:2:3:1.

Experimental Example 5: Test of wound improvement or therapeutic effect through gross morphological analysis of the composition according to the present invention

After dispensing the prepared HaCaT cells at a concentration of 2.5 x 10 ⁵ per well of the cell culture vessel and culturing for 248 hours to form a cell monolayer, the culture medium was removed and “scratch-damage” was induced with a p200 pipette tip. A culture medium containing the plant extract obtained in Example 2 was added to the cells induced in "scratches-damage", and then cultured for 48 hours.

In the present invention, the degree of recovery of the scratch wound was confirmed after each treatment of the extract at a concentration of 50 μg/ml in the “scratch-damaged” cell layer for 48 hours using an in vitro woound healing assay. The control group was treated with the same amount of DMSO in which the extract was dissolved.

As a result of the experiment, the scratched area was hardly reduced in the composites 4:3:2:1, 4:2:3:1, 3:3:3:1, and 50 μg/ml of the composite 4:2:2:2 In “scratch-damage”, it can be confirmed that the scratched area is reduced after 48 hours.

Experimental Example 6: Moisturizing effect test of the composition according to the present invention

A Hyaluronidase Inhibition Activity Test was performed as a test for moisturizing the skin using the plant extract obtained according to Example 2 as a sample.

The activity inhibition experiment of the hyaluronidase enzyme was performed as follows. 0.05 ml of hyaluronidase (7,900 unit/ml) dissolved in 0.1M acetate buffer (pH 3.5) and 0.1 ml of the sample solution were mixed and reacted at 37°C for 20 minutes, then 0.1 ml of 12.5 mM calcium chloride (CaCl ₂ ) was added and mixed. Then, it was reacted again for 20 minutes. As a control group, 0.05 ml of hyaluronidase (7,900 unit/ml) and 0.1 ml of distilled water were mixed instead of the extract, and hyaluronic acid (hyaluronic, HA; 12 mg/ml) dissolved in 0.1M acetate buffer (pH 3.5) as a substrate was added. After reacting for another 40 minutes, 0.1 ml of 0.4 N potassium tetraborate and 0.4 N NaOH solution were added to the 0.1 ml reaction mixture, heated in a water bath for 3 minutes, and then completely cooled. 3 ml of p-dimethylamino benzaldehyde (DMAB) reagent, a color developer, was added to the cooled reaction mixture, reacted at 37° C. for 20 minutes, and absorbance was measured at 585 nm.

As a result of the experiment, it was confirmed that the hyaluronidase activity was inhibited in the complexes 4:3:2:1, 4:2:3:1, 3:3:3:1, 4:2:2:2 compared to the control group. , the most excellent effect was confirmed in the complex 4:2:2:2.

Looking at the results in FIGS. 1, 2, 3, 4, 5 and 6, which are the efficacy results of the extract extracted at a low temperature of 40°C, in the complex extract, 4:2:3:1, 4:3:2 :1, 4:2:2:2 extract showed antioxidant, whitening, anti-wrinkle, anti-inflammatory and moisturizing effects, and 4:2:3:1 showed better antioxidant, whitening, anti-wrinkle and anti-inflammatory effects. . However, the wound improvement and moisturizing effect showed the best effect at 4:2:2:2.

According to the present invention, the bath composition containing green tea, centella asiatica, mugwort, and ginger mixed powder as an active ingredient is excellent in antioxidant, whitening, wrinkle improvement, wound healing and moisturizing effects, and is non-toxic, so it is a natural bio material for cosmetics or food. It can be widely used as a material for

In addition, since it provides an excellent natural biomaterial composition, it is very useful in the bio-bio food/pharmaceutical industry.

Claims (6)

A bath composition comprising 30-40 wt% of green tea powder, 20-30 wt% of Centella asiatica powder, 20-30 wt% of mugwort powder, and 10-20 wt% of ginger powder. The bath composition according to claim 1, wherein the bath composition is used for any one of whitening, wrinkle improvement, anti-inflammatory, moisturizing, and preventing or improving skin wounds. A cosmetic composition comprising 30-40 wt% of green tea extract, 20-30 wt% of Centella asiatica extract, 20-30 wt% of mugwort extract, and 10-20 wt% of ginger extract. The cosmetic composition according to claim 3, wherein the cosmetic composition is used for any one selected from among whitening, wrinkle improvement, anti-inflammatory, moisturizing, and preventing or improving skin wounds. A food composition comprising 30 to 40% by weight of green tea extract, 20 to 30% by weight of Centella asiatica extract, 20 to 30% by weight of mugwort extract, and 10 to 20% by weight of ginger extract. The food composition according to claim 5, wherein the food composition is for any one use selected from among whitening, wrinkle improvement, anti-inflammatory, moisturizing, and skin wound prevention or improvement.

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