KR20080101821A - Cosmetic included to flos - Google Patents

Abstract

A composition for skin external application is provided to prevent and improve skin disease by including carthami Flos fractions, to be used as liquid, cream and ointment, to improve rough skin, atopic disease skin, black skin, and dry skin, to perform washing, moisturizing, and whitening functions and to remove wrinkles. A composition for skin external application includes Carthamus tinctorius L extract and a carrier allowed to cosmetology. The composition for skin external application additionally includes one or more selected in a group consisting of sulfur, Aloe, Mori Radicis Cortex, Phellodendri Cortex, Angelicae dahuricae Radix, Rhodiola sachalinensis, Aconiti koreani Tuber, Dictamni Radicis Cortx, Alum, Rosin, Pearl powder, Cholesterol, Talcum, fed wild ginseng, gypsum. Borax and silver.

Description

Skin external composition comprising safflower fractions {Cosmetic included to FLOS}

The present invention relates to an external preparation composition for skin, comprising safflower fractions, and more particularly, an alkaline calcium solution or potassium hydroxide or liquefied potassium extracted from a shell as a main solvent, and the fraction extracted from safflower as a main substrate of the present invention. The external preparation composition for skin containing.

In addition, the present invention is a skin external preparation having a liquid, cream, ointment form by additionally preventing and improving skin disease, whitening, moisturizing, and cleaning functional substances, and improves rough skin, atopic skin, dark skin, dry skin, The present invention relates to an external preparation composition for skin, comprising a safflower fraction, which is characterized by promoting washing, moisturizing, whitening, and wrinkle improvement.

Calcium powder is prepared using a shell, a method of extracting a functional active substance by mixing the calcium powder and a functional substance of a herb and impregnating it in purified water, and applying electricity to a precious metal as a catalyst for the safety of the calcium alkaline solution and potassium hydroxide solution. When the acid is reacted with the alkali, the substance dissolved in the alkali lowers the reduction rate to a solid, and relates to a method of mixing an acid stabilized safflower or an extract of an additive.

As economic growth grows, we are rich in diets, and many chemicals are naturally absorbed into our bodies.

As a result, toxins accumulate in the body and due to the excretion of the toxins, diseases accompanied by pruritus are generated on the skin.

Also, in order to pursue beauty with satisfaction of life, many cosmetics for beauty are provided.

Many cosmetics are being provided according to the development of cosmetics, but many cosmetics have been developed to solve skin diseases and beauty at the same time, but have not yet reached a satisfactory level.

Skin external preparations containing safflower extract have little research, they will.

The study of safflower was carried out in 1995 by Shimokoriyama et al., A study of carthamin as a pigment, and in 1988, by Goda, Nakano, etc., the component analysis was carried out using HPLC of the pigment, and in 1997 Watanabe et al. Therefore, in 2005, Jiang et al. Attempted a pigment separation study for clinical pharmacological effects.

In 1993-2003, Kim, Yoon and Spyridon studied and reported the change and safety of carthamin according to ph and temperature change.

In 1997, Zhang et al., N- [2- (5-hydroxy-1H-indol-3-yl) eth1] ferulamide, N- [2- (5-hydroxy-1H-indol-3-yl) from safflower oil. eth1] -p- coumaramide, N, N '-[2,2'-(5,5'-dihydroxy-4,4'-bi-1H-indol-3,3'-y1) diethyl] -di-p -coumaramide, N- [2- [3 '-[2- (p-coumaramide) ethyl] -5,5.-dihydroxy-4,4'-bi-1H-indol-3-y1] ethy1] ferulamide, N , N '-[2,2'-(5,5'-dihydroxy-4,4'-bi-1H-indol-3-y1) diethl] -diferulamide (5), N- [2- [5- ( beta-D-glucosyloxy) -1H-indol-3-yl] ethyl] -p-coumarmide, N- [2- [5- (beta-D-glucosyloxy) -1H-indol-3-yl] ethyl] ferulamide Separated,

In 1998, 6-hydroxy kaempferol 3-O-glucoside, 6-hydroxy kaempferol 7-O-glucoside, kaempferol 3-O-glucoside and querercetin 3-O-glucoside were isolated by Li Y. in China, and in 1999, by Srinivas et al. Alpha linolenic acids, palmitic acide, gamma linolenic acide, decanoic and dodecanoic acides were identified in safflower petals.In 2003, Tsutomu Kanehira et al. studied the antioxidant component of Kinobeon A through cell culture of safflower, and in 2006, Zhou et al. 4 ', 6'-asetonide-8Z-decaene-4,6-diyne-1-O-β-D-glucopyranoside was isolated.

However, there is still no provision of a pharmaceutical composition comprising the above substances, and research is actively underway in the medical and pharmaceutical fields.

Accordingly, the present invention has an object of providing a topical agent for the prevention and amelioration of the disease of the skin and a cosmetic using a comprehensive mixture of the above substances.

In addition, the composition that achieves the whitening effect of sulfur, lucifer of fir extract, mold extract coenzyme, coenzyme Q-10 as a free radical inhibitor, and placenta for wrinkle improvement and whitening, etc. Herbs and the like, including animal and mineral external preparation composition has been provided.

Changyangza has been provided with a composition using the extract as a cosmetic for improving pruritic skin diseases.

The conventionally provided compositions have tried to remove pruritus by removing free radicals, but have no effect or satisfactory effect depending on the chemical composition of the body fluid.

In addition, the extraction of the functional active material from the existing herbs and animal substances by extracting the method by impregnating with alcohol, or by impregnating with purified water to extract the active material. However, the alcohol extraction method is extremely limited in using the active material contained in alcohol, and the active material is obtained by distilling alcohol into a powdering method, and the active material made of powder is dissolved in purified water again and used in external preparations. In addition, in order to obtain a skin cosmetic functional substance including a cleaning function, a technique of extracting the active substance by an acidic or alkaline solvent is provided and widely used. However, functionally active substances extracted by the use of existing acidic or alkaline solvents put a heavy burden on the skin.

In addition, by using a known solution such as calcium hydroxide alkaline solution as a solvent safflower, liquefied sulfur with an additive. The solution of safflower and sulfur liquefied using potassium hydroxide as a solvent causes skin irritation, and when acid is mixed in a neutralization process to reduce the irritation, the safflower extract component and sulfur are solidified again.

In addition, there is a problem in that the effective substance is decomposed by heat and the effectiveness is reduced when the fraction obtained from the existing herbs is impregnated with water and heated.

Accordingly, the present invention supplements the above problems, and maximizes the whitening effect, the improvement effect of wrinkles in the prior art, including atopic skin disease, eczema skin disease, psoriasis, ringworm, acne, pruritus and the like. It aims to prevent or improve various skin diseases, and to provide an external preparation for skin that has an excellent effect on the effect of whitening and prevention and improvement of wrinkles.

In order to achieve the object of the present invention, the inventors have been studying the treatment or improvement of the main symptoms of skin diseases pruritus, inflammation, erythema, dead skin, blotch, blemishes, etc. It improves the action and blood circulation, and it is excellent in free radical scavenging and skin whitening function, and it is treated with shells, ionized strong alkaline calcium solution extracted from waste coral or sulfur dissolved in potassium hydroxide or potassium iodide to treat skin diseases. We found that the effect was improved.

The harmless strong alkaline calcium solution has no side effects of irritating the skin, has a strong bactericidal power and cell regeneration effect, and has been found to have a strong extraction power of a substance having a functional effect of whitening, moisturizing, and elimination of pruritus.

When extracting the herb extract, it was found that the extract extracted with solvents such as agglomerate calcium ion alkaline solution, potassium hydroxide, potassium iodide, MeOH, etc. was excellent for free radical scavenging.

Accordingly, when the waste calcium ions alkaline solution, potassium hydroxide, potassium iodide solution MeOH is used as a solvent, and the functional materials including safflower are impregnated in the solvent and heated, the extract is quickly extracted and the precious metal is used as a catalyst. When the noble metal was attached to the electrode and applied with electricity, the sulfur solution dissolved in the solvent was found to have a low rate of reduction of the sulfur solution into a solid by acid.

Therefore, the present invention extracts a strong alkaline ion calcium solution of ph 10 or more from the lung shell, and using the calcium solution or potassium hydroxide, potassium iodide, MeO as a solvent base, impregnating the safflower with the safflower extract, and thus adding Water is liquefied or extracted and further included in the safflower extract to prepare a skin cosmetic composition in cream, ointment and liquid form.

And the additional product is selected from any one or more of the herb extracts of herbs, any one or more extracts of animal extracts, minerals or extracts of any one or more of the extracts, or the herb or its extracts, animal substances or extracts, minerals or extracts thereof. Any one or more mixtures may be mixed with the composition to prepare a skin external preparation for preventing and improving skin diseases, skin whitening, wrinkle improvement, and skin cleansing.

In the past, alcohol was used in the process of liquefying to use the alkaline calcium ions for drinking, and sulfur and purified water were used simultaneously to prepare edible sulfur. However, it is accompanied by pain when contacted with alkali solution or alcohol on the skin wound.

Accordingly, another object of the present invention is to prepare a strong alkali calcium solution by extracting alkaline calcium ions with purified water, and to extract the active substance from the herb with the calcium solution, or simultaneously calcined powder and herb of the shell purified water. Method of obtaining a solution containing the functional active substance of the herb at the same time by heating it by impregnation or impregnating potassium hydroxide with safflower or functional herb in purified water, applying electricity and adding citric acid to adjust the acid to extract the effective substance. Its purpose is to provide a method.

Therefore, the purpose of neutralizing the pathological acid skin is another object to provide a weak alkaline cosmetics do not burden the skin at all.

According to a feature of the present invention for achieving the above object, the present invention is a skin external preparation having the purpose of improving or treating skin diseases including safflower fraction, whitening, moisturizing, aging prevention and wrinkle improvement, the external preparation for skin Is a support of safflower fraction in the form of liquid or cream or ointment and is a composition comprising 0.1% -20% by weight of safflower fraction in a pharmaceutically and cosmetically acceptable carrier.

In addition, sulfur, aloe, baekbaekpi, honggyeongcheon, baekbaek, baekbuja, white paper, alum, rosin, pearl powder, cholesterol, talc, camphor, ringworm, gypsum, borax, and silver as an additive to aid the functional enhancement of the composition. One or more substances selected from the group may be included in addition to the composition.

For example, safflower extract 0.1% -20%, sulfur solution 0.03% -10%, aloe extract 0.1% -20% by weight in 100 parts by weight of a pharmaceutically or cosmetically acceptable carrier. Morus extract 0.1% -20% weight. Rhodiola sac extract 0.1% -20% weight. Yellow-white extract 0.1% -20% weight. White paper extract 0.1% -20% by weight, white rich extract 0.1% -20% by weight, ringworm skin extract 0.1g-20g by weight, alum 0.1% -10% by weight, rosin 0.1% -10% by weight, pearl powder 0.1% -10% weight part, cholesterol 0.1% -10% weight part, gypsum 0.1g-10g weight part, talc 0.1% -10% weight part. 0.1 g-10 g weight part of borax, 0.1% -10% weight part of camphor, and 0.001% -2% weight part of silver.

In the term of the present invention, the fraction of safflower is an extract extracted from safflower seeds, stems, roots, leaves, collectively referred to as safflower fraction. Pasansan coral is a calcified coral, shells means shells of shellfish, shellfish, paternity, abalone and oysters that live in fresh or marine water.

The composition may further include any one or more kinds of herbal extracts selected according to the functionality of the herbal extract as an additional.

For example

100 parts by weight of a composition in the form of an ointment or cream or solution comprising a carrier and a safflower fraction, trichophyllum, sorghum, aloe, honeysuckle, cheongdae, horsetail, chickweed, comfrey, persimmon leaf, rice bran, mulberry, cocoon, eggplant. Rat melon. Tenza. apricot. Peach leaf or doin. Dong-ah. Pomegranate. Sidelines. Acacia, Schizandra. Plantain. Bear smell. Centennial, Pogongyoung. Gold. Kwonbaek. Brotherhood. goldthread. Gold. frankincense. Gyeji, Angelica, Machi, Rhubarb, Ginkgo, Garlic, Carrot, Yulmu, Centella, Green onion, Grapefruit, Perennial, Injin, Green tea, Rose of sharon, Tempered chrysanthemum, Hoi cho, Cactus, Ratberry, Chrysanthemum, Chestnut, Tare, Ginseng , Willow, walnut, plum, Changzai, Uijin Forsythia, Balsam, red ginseng, purslane, lake, jinbum, pandanche, cicada, hyunsam, chichi, nippon, rose, grafting tree , ringworm, ash , half , Evening Primrose Oil, Centella asiatica, Hamcho, Licorice, Harpoon, Herb, Peony, Lily, Yeongneung, Cypress, Burdock, Apple, Grapes, Fruits, Mushrooms, Beans, Sasam, Lichen, Platinum, Norin Wood, niglospores perica, vinewood, carnation, collardecavalo, beetle, walnut caridica, astaxanthin, acerola spp, starfish, pine, birch, sugar, fir, chemomile, marigold, rosemary , Peppermint, lemon balm, Douglas, jasmine, propolis, allantoin, tea tree, Any one or more kinds of extracts selected from lavender or maroni may be included as an additive.

Alternatively, the composition may further include any one or more kinds of animal extracts selected from animal extracts such as sesame (cicada shell) and earthworm extract.

Alternatively, the composition may further include any one or more kinds of minerals or extracts thereof selected from minerals or extracts thereof.

Alternatively, any one or more functional active substances selected from the herb extracts, any one or more functional active substances selected from animal extracts, minerals or any one or more functional active substances selected from the extracts may be mixed.

For example, 0.05% -7% by weight of the functional material selected from animal materials in 100 parts by weight of the composition containing the safflower extract. 0.01% -5% of the functional material selected from the mineral material may be further included.

Or 0.05% -5% of vitamin C as a vitamin, and 0.001% -2% of citric acid as a dispersant in the composition of Ph and the composition, to quickly absorb calcium ions into the composition. Can be.

Or a composition containing safflower fraction in a carrier containing calcium ion alkaline solution or potassium hydroxide or potassium iodide or MeOH solution in the lung shell, or sulfur, lettuce extract, white paper extract, sulfur white extract, red pepper extract, alum and rosin 0.3% -15% by weight of rucinol as a whitening agent in the composed composition 100. Terrain 0.3% -15% by weight. Coemzyne-Q10 parts by weight, arbutin 0.2% -2% by weight may be included as an additive.

Functional in the above means the function of improving the skin disease, whitening function, moisturizing function, cleaning function, wrinkle improvement prevention.

The carrier of the composition of the present invention is 5% to 45% stearic acid. In the form of a liquid, cream or ointment containing 5% -45% glycerin.

For example, 5% -45% of Stearic Acid to 100% calcium alkaline solution of ph 10-15 containing safflower fraction. 0.5% -1.5% of cellulose and 0.2% -0.7% by weight of Ar-165 in a mixture composed of 5% -45% of GLYCERIN. Lanette 1665 0.5% -2% by weight. GMS-105 0.3% -0.7% weight. GMS-205 0.3% -0.7% weight. D-P 0.08% -0.3% by weight. Tween 60 0.5% -1.5% weight. LP # 70 1% -10% weight. IPM 1% -6% by weight. SILICONE200 / 100CS 0.1% -1% weight. ALLANTOIN 0.01% -0.08% weight. Clofenensin 0.5% -5% by weight. PG 1% -5% by weight. 1% hyaluronic acid 0.1% -5% by weight. CARBPOL 941 0.1% -2.5% by weight. MSM 2% -5% weight, ceramide 0.1% -10% weight part, collagen 0.1% -10% weight part Kondracin 1% -10% weight part. 1% -10% by weight of elastin. At least one selected from the group consisting of materials may be mixed.

And or cetyl alcohol, water solubilizer, cetos KD, olive oil wax, montan wax, emulsion wax. lecithin. beeswax. Olive Liquid. Polyquarter. High cell. 젱 Tan gum. Cellulose, glycerol stearate. One or more substances of RMA, may be composed of a mixture consisting of 0.05% -2% additionally mixed in the carrier composition.

Referring to the components of the present invention in detail as follows.

The method of extracting strong alkali calcium ions from the waste shell is a conventionally known technique, which utilizes a technique of pulverizing the waste shell by pulverization, and impregnating the powder with purified water and heating it to a temperature of 90 degrees Celsius or more to extract a calcium ion solution. Calcium ions are extracted from the waste shell and can be used as a solvent for preparing a carrier using liquefied calcium ions.

An effective substance may be extracted by impregnating the at least one herb in the extracted pulmonary calcium ion alkaline solution or potassium hydroxide solution, or potassium iodide solution or MeOH solution. The extract of the herb is an alkaline herb solution.

Alternatively, citric acid may be dissolved in purified water to obtain a citric acid solution, the citric acid solution may be impregnated with safflower to obtain a safflower extract solution to obtain a safflower extract solution containing an acid, and the pH of the alkaline mixed solution may be adjusted, or a weakly acidic external preparation may be used. It can manufacture.

An important component of the composition of the present invention will be described as follows.

Calcium solutions are strong alkalis with a pH of 10-15. In general, alkalis of this level are immediately damaged when in contact with the skin, and severe pain when in contact with the wound. However, the calcium solution extracted from the lung shell does not damage the skin at all, even though it is strong alkali, and has no pain when contacted with the wound. Therefore, a large amount of contact with the skin has no side effects.

The calcium solution is quickly extracted in extracting the active substance of the herb with strong alkali. And strong alkali has a strong sterilizing power.

Citric acid is one of the polybasic carboxylic acids having a hydroxy group. The chemical formula is C6H8O7, which exists freely in the seeds or juices of plants. One component of the TCA circuit is an important role in metabolism in higher animals and also promotes the absorption of calcium.

Safflower is a safflower belonging to the compositae, and its scientific name is carthamus tinctorius L. It is a flower of annual vegetation.

The flower is nontoxic and improves blood circulation, activating blood, and acts as an antioxidant to eliminate free radicals, removes impurities from the skin, helps to supply the skin, and cleans the skin. . It also works to reduce heat, to calm hysterical mood and to relieve pain.

Looking at the action of the addition as follows.

Sulfur works with organic substances to form pentathiic acid (HO-SO2-S3-SO2-OH), which dissolves keratin harmlessly to the human body and acts as a pesticide. Sterilizes and removes the causative bacteria, bacteria, viruses, bacteria and fungi of spear, lower window, nematode nematodes, scabies and ringworm, and removes poison from the skin. Sulfur has a strong pain relief action, strong substance binding and transporting properties to penetrate the composition of the present invention deep into the cell membrane to exert weakness, strong interlocking action to prevent skin aging, and to regenerate cells quickly It reduces scar tissue of lost skin and has strong antioxidant activity, releasing the dissolved blood of damaged skin and promoting blood circulation. Sulfur is the eighth vital necessity in the human body and contains about 140 g.

In addition, sulfur is deeply involved in skin tissue's ability to purify (wrinkle, elasticity), the keratin protein in the epidermal layer prevents the loss of water to maintain moisture. It detoxifies harmful substances deposited on the epidermal tissue and maintains skin elasticity by sticking together various amino acids constituting collagenase enzyme directly involved in collagen molecules that contribute to skin elasticity. Sulfur is a water-soluble solution that is legally formulated to be sulfur. Water Soluble Dietary Sulfur is harmless, nontoxic and no side effects.

The amount of sulfur added may be increased by increasing the value of dietary sulfur extracted from pine and detoxified sulfur. Especially, skin shingles should contain as much sulfur as possible. The amount of water-soluble sulfur used in the skin of infants and children under adolescence is added at a low level.

Aloe refers to the entire plant belonging to the genus Aloe, has bactericidal power against bacteria and fungi, contains aloetin, which neutralizes toxins, and contains aloeurcin, which is effective for ulcers, steroids, amino acids, saponins, antibiotics, It contains wound healing hormones and minerals, and neutralizes the neutrality and oiliness of the skin, and has the effect of moisturizing the skin.

White paper (Radix Angelicae Dahuricae) is a root of the perennial herbaceous perennial herbaceous plant, which is non-toxic and helps skin whitening like safflower.

Cortex phellodendri is a cork layer of cork from the Rhaceae family. It is not toxic and has strong antibacterial properties, helps to whiten the skin and has a strong preservation of substances.

Among the antibiotics such as aminoglycoside, tetracycline, pulmonary nicillin, cephalosporin, macrolide, polypeptide, lincosamide, sulfonamide, fluoroquinolone, etc. More than one type of antimicrobial may be included.

Rhodiola is a Rhodiola sachinensis A. Bor, a perennial herbaceous plant belonging to the Crassulaceae family. It is not toxic and has anti-toxic effects on bacterial toxins and promotes skin metabolism.

Referring to the manufacturing method of the present invention will be produced in the following steps.

1.The waste angle is fired at 1000 degrees Celsius-1500 degrees Celsius directly and indirect heat for 1 minute-30 minutes

2. Put the powder into purified water and heat it for 5-30 minutes with 100-120 degree Celsius heat.

3. The heated powder is separated by a filter and a centrifuge to obtain a solution.

4. Add safflower to the solution and extract the active substance from safflower by adding temperature below 70 degrees Celsius. In this case, the solvent may be a calcium hydroxide, potassium iodide, or MeOH solution, rather than the waste calcium ions alkaline solution.

Alternatively, safflower is added to citric acid solution in which citric acid is dissolved in purified water and heated at a temperature of 70 degrees Celsius or less for 2 to 30 minutes to obtain safflower extract. The extract is a safflower extract solution of citric acid.

5. The alkaline safflower extract or citric acid safflower extract, or a mixed solution of alkali and citric acid extract is mixed with a carrier material at a temperature of 70 degrees Celsius or less and stirred to obtain an external skin preparation including safflower.

And the addition method of the addition to the external preparation including safflower is as follows.

1. Impregnated with an alkaline aqueous solution, an aqueous potassium iodine solution, or a MeOH solution obtained in the above 3, an herb having an alkali affinity as an ingredient in the additives, extracts the active substance in the above 4 step.

2. An acid-affinity herb is impregnated with an aqueous solution of alum or purified citric acid solution and heated to a temperature of 70 degrees Celsius or less to obtain an effective extract.

3. Mix the alkaline alkaline herbal extracts into one container, and mix the acidic herbal extracts into one container to obtain a mixed solution,

4. Place the +-electrode wire in the container. Then, a platinum group precious metal plate was attached to one of the + -electrode lines, and a stainless steel plate was attached to the other electrode line. Then, sulfur was added thereto, followed by stirring. Get

5. As an additive, dissolve substances such as rosin and camphor in ethyl alcohol to obtain a solution.

6. Slowly mix the alkaline mixed solution and acidic mixed solution with the carrier active material to keep the pH neutral, heat and stir at a temperature below 70 degrees Celsius, add the dissolved solution in sulfur solution and ethanol, and then add 70 degrees Celsius. After heating for 2 minutes to 10 minutes with the following heat, an alkaline safflower extract or citric acid safflower extract is put and stirred. The ph is maintained at 5-8.

7. The heat of 6 is cooled and stored in a container.

Therefore, according to the external preparation composition for skin according to the safflower extract of the present invention according to the above-mentioned solutions to the problem, it is effective in the improvement and moisturizing, cleaning, whitening of atopic pruritus, and has the effect of promoting the health of the skin without any side effects.

Hereinafter, the specific details for carrying out the skin external preparation composition containing the alkaline calcium solution of the shell according to the present invention will be described.

The present invention is an external preparation for skin having a liquid, cream and ointment formulation, wherein the external preparation is used in a carrier 100 in which the alkaline calcium solution of ph10-13 extracted from the lung shell contains 5% to 95% by weight of the total weight of the external preparation for skin. The structure is characterized by including 0.3% -20% by weight safflower.

Extraction of the active substance of safflower is performed by extracting MeOH, extracting with CHCl3, and performing column chromatography using Amberlite XAD-2 to obtain a water fraction and a MeOH fraction, which are mixed with a carrier.

Obtaining a strong alkali solution from the shell and impregnating the safflower with the solution to prepare a carrier component in the extracted solution,

Potassium hydroxide was added to purified water to obtain a potassium hydroxide solution, or potassium iodide was dissolved in purified water to obtain a solution, and safflower was impregnated into a selected solution of the two solutions to obtain a safflower extract solution. How to. At this time, the toxic metal is applied to the potassium hydroxide solution with electricity to lower the toxicity.

After citric acid is added to purified water to prepare a citric acid solution, the citric acid solution is impregnated with safflower and heated to extract the safflower active material, and the safflower extract includes a carrier and the like.

Potassium hydroxide, potassium iodide, citric acid or potassium alum can be used for neutralization of acid and alkali.

Alkaline calcium solution is an extract of the shell in the present invention is an important component responsible for sterilization, pruritus improvement, cell regeneration in the present invention, and also serves as a solvent to dissolve and extract the various components formulated in the composition of the present invention.

And safflower acts on blood vessels existing in the cells of the skin and acts to personalize blood circulation.

Shell shells are collectively called fresh shells, marine shellfish, abalones, shellfish, oysters, corals, mussels, etc., which are found in freshwater and in the ocean.

Shell powder contains a large amount of calcium as a strong alkaline material. Strong alkali solution absorbs large amount of oxygen to supply oxygen to diseased cell, and it is involved in normalization of autoimmune function by antioxidant activity, and it has the function of cell regeneration and pruritus improvement, and it is harmless and harmless even when it comes in contact with wound. It has strong sterilizing power and has the ability to liquefy sulfur.

In addition, the composition for external application of the skin of the present invention is a general-purpose cell regeneration material consisting of plant extracts, seaweed extracts, animal extracts, minerals or extracts thereof, chemicals or compounds thereof, antibacterial substances, bactericidal substances, moisturizing substances, nutritional substances, wrinkle improvement It may further include one or more components selected from the group consisting of substances, antipruritic substances, analgesics, hormonal agents, blood circulation promoters, and anti-arregic substances.

The composition further comprises 0.1 to 30.0 parts by weight of the plant extract based on 100 parts by weight of the carrier, the plant extract is a solvent extracted with the at least one solvent selected from the group consisting of water, aqueous alkali solution and C1-C4 alcohol Extracts, or plant essential oils.

Three hundred vines (eosungcho), zebra, aloe, honeysuckle, blue, baekbuja, horsetail, chickweed, comfrey, persimmon leaf, rice bran, mulberry, cocoon, eggplant. Rat melon. Tenza. apricot. Peach leaf or doin. Dong-ah. Pomegranate. Sidelines. Acacia, Schizandra. Plantain. Bear smell. Centennial, Pogongyoung. Gold. Kwonbaek. Brotherhood. goldthread. Gold. frankincense. Gyeji, Angelica, Machi, Rhubarb, Ginkgo, Garlic, Carrot, Yulmu, Centella, Green onion, Grapefruit, Perennial, Injin, Green tea, Rose of sharon, Tempered chrysanthemum, Hoi cho, Cactus, Ratberry, Chrysanthemum, Chestnut, Tare, Ginseng , Willow, Hodo, Plum, Changzai, Uijin Forsythia, Balsam, Red Ginseng, Purslane, Lake, Genuine, Panther, Black Root, Hyunsam, Chichi, Japanese Gooseberry, Rose, Graft, White Line, Ash Tree , Evening primrose, evening primrose oil, centella asiatica, seaweed, licorice, harpoon, vinegar, peony, lily, youngneunghyang, cypress, burdock, apple, grapes and other fruits, mushrooms, legumes, samsam, sapling, platinum , Lining tree, niglospores perica, marsh tree, carnation, collarde cavallo, beauties, walnut caridica, astaxanthin, acerola spicy, starfish, pine, birch, sugar tree, fir, chamomile, marigold Rosemary, Peppermint, Lemon Balm, Douglas, Jasmine, Propolis, Allantoin, Tea Tree, Lavender, horse chestnut extract of any one or more selected 0.1% -30% may be included as an additive.

The composition for external application for skin is a group consisting of nonsteroidal flufenamic acid, ibuprofen, ibuprofen, benzydamin, indomethacin, and steroid-based prednisolone dexamethasone. One or more anti-inflammatory agents selected from may further comprise 0.0001-2.0 parts by weight based on 100 parts by weight of the carrier.

The external preparation composition for skin is Aminoglycosides, Glycopeptides, Tetracyclines, Spectinomyxin, Bacitracin. One or more antibiotics selected from the group consisting of Polymyxin, and Fosfomyxin may further comprise 0.0001-2.0 parts by weight based on 100 parts by weight of the carrier.

The external application composition for skin may further include an extract extracted from an earthworm, a frog, a lizard, a snake, a centipede, an ant, an animal skin, an organ, or a placenta.

Seaweeds also include seaweed, kelp, coral, and seaweed. Calcinin, arginic acid, sodium arginate, potassium arginate and the like purified from seaweed extracts are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

Examples of minerals that can be added to the compositions of the present invention include silver, mica, gold, calcium, silicic acid, loess, marine clay, and the like.

Referring to the example of the method for producing a composition for external application of the skin of the present invention will be described. Alum is dissolved in water, sap, plant extract or deep sea water to prepare a solution, sulfur is dissolved in an aqueous alkali solution to prepare a solution, and the alum and sulfur solution are injected into a porous carrier material, followed by adsorption. Can be. The alkaline solution used to prepare the sulfur solution can be prepared from an aqueous solution of alkali metal or plastic shell of shellfish, abalone, shellfish and coral.

Shell powder is a strong alkaline substance, mostly containing calcium. The strong alkaline solution extracted from the lung shell absorbs a large amount of oxygen to supply oxygen to the diseased cell, and is involved in the normalization of autoimmune function through antioxidant activity, and has the function of cell regeneration and pruritus improvement, painless even when contacted with wounds, Harmless, harmless, strong bactericidal, has the function of liquefying silver and sulfur.

A solvent may be used when preparing the external preparation composition of the present invention, and examples of the solvent may include water, tree sap, and deep sea water.

The external composition for skin of the present invention may be a pharmaceutical composition. Compositions of the present invention generally also include a pharmaceutically acceptable carrier. Carriers for topical application, useful in practicing the present invention, include, but are not limited to, alkylene glycols or combinations of one or more derivatives of alkylene glycols and hydroxyalkylcelluloses.

After the formulation of the present invention is formulated, the solutions will be administered in a therapeutically effective amount in a manner compatible with the dosage formulation. The formulations are easily administered in a variety of dosage forms (eg, direct topical application, application via transdermal patches, etc.). The dosage can be appropriately selected in consideration of the condition of the skin disease.

The external composition for skin of the present invention may be a cosmetic composition. The cosmetic composition of the present invention is a skin gel, cream, lotion, powder, foundation, essence, gel, pack, foam cleansing, soap, skin ointment. Soap, cleansing, oil, powder foundation, emulsion foundation, or wax foundation formulation.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, the composition is vitamins, amino acids, proteins, surfactants, emulsifiers, stabilizers, thickeners It may further comprise one or more additives selected from the group consisting of preservatives, moisturizers, antioxidants, fragrances, pigments, stabilizers and preservatives.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, any of the above components can be mix | blended within the range which does not impair the objective and effect of this invention.

In the firing of shells, precious metals such as platinum, paradium and rhodium or rare earth metals such as cerium may be used as catalysts when extracting the components of the shells.

Example. One.

Produce.

Ingredients: Safflower 5500g, Andong, Korea 1kg freshwater scallop shell, 50g citric acid, 4,000ml purified water

Additional material including carrier: 450 g stearic acid, 450 g glycerin. 30 g of fiber, 14 g of Ar-165. Lanette 1665 40 g. 14 g of GMS-105. 14 g of GMS-205. D-P 6g. Tween60 30g. LP # 70 200 g. IPM120g. SILICONE200 / 100CS 20g. ALLANTOIN 1.6g. Clofenensin or benzoic acid 100 g. PG 100 g. 40 g of 1% hyaluronic acid. 100 g of ceramide. 100 g collagen, 100 g corndracin, 80 g elastin. CARBPOL 941 50 g. MSM 100g. 20 g of sulfur, 100 g of aloe, 100 g of alum. 100 g of borax, 100 g of pearl powder, 100 g of talc, 100 g of gypsum, 100 g of baekbaek, 100 g of yellow white, 100 g of red ginseng, 100 g of white paper, 100 g of ringworm, 100 g of cholesterol, 100 g of silver, 100 g of 100% of rosin. Prepare 100g of camphor.

Implementation for the specific process of manufacture

1. MeOH was added to 500g safflower, extracted three times at room temperature for 2 weeks, and then concentrated under reduced pressure to obtain an extract. The MeOH extract was suspended in distilled water, and extracted with CHCl3. The mixture was extracted repeatedly with a funnel, and the CHCL3 layer and the aqueous layer were separated. After fractionation, the resultant was concentrated under reduced pressure to obtain 16 g of CHCL3 extract. The remaining water layer (65 g) was subjected to column chromatography using Amberlite XAD-2 to obtain 6 g of water fraction and 1.3 g of 30% MeOH fraction, respectively. Carrier composition 100 g-450 g stearic acid, glycerin 100 g-450 g. 10g-30g of fiber, 4g-14g of Ar-165. Lanette 1665 weighs 10g-40g. GMS-105 6g-14g weight. GMS-205 6g-14g weight. D-P 1.6g-6g weight. Tween60 10g-30g weight. LP # 70 20g-200gweight. IPM 20g-120g weight. SILICONE200 / 100CS 2g-20g weight. ALLANTOIN 0.2g-1.6gweight. Clofenensin or benzoic acid 10 g-100 g weight. PG 20g-100g weight. 2 g-40 g weight of 1% of hyaluronic acid. Ceramide 2g-100g. 0.5-8 g collagen, 0.5 g-8 g of corndracin, 0.5 g-8 g of elastin. CARBPOL 941 2g-50g weight. MSM 40g-100g weight. A skin external preparation was prepared by mixing sulfur with a carrier composed of 0.3g-20g aloe extract 1g-100g.

2. Prepare 1,000g of freshwater algae selected from waste shellfish and Pasan lake, and put it in a heating furnace with a space of 0.2m3 made of magnetite brick, and direct heat to 1500 ℃ or more using oxygen and LPG gas. Heat the chopped tea in a minute to 30 minutes.

3. Put the heated mulsel in a grinder to grind and filter under high pressure to obtain 100 mesh-2,000 mesh powder.

4. Immerse 100 g of the powder in 2,000 cc purified water and heat for 5-30 min with 100-150 degree Celsius heat.

5. Separate the third heated purified water and crushed powder with a filter to obtain an alkaline solution of ph10-13.

6. a. Impregnated with safflower 10g-30g in 1000ml of the solution and heated to a temperature of 70 degrees Celsius or less for 3 minutes-5 hours to obtain safflower extract.

b. 1.5 g-3 of potassium hydroxide is added to 500 ml of purified water, and 0.5 g-15 g of safflower dry matter is added to 500 ml of liquefied potassium hydroxide solution or potassium iodide and heated to a temperature of 70 degrees Celsius or less to obtain a safflower extract.

c. In addition, 0.5g-30g of citric acid was dissolved in 1000ml of purified water and impregnated with 10g-30g of safflower in the citric acid solution, and heated to a temperature of 70 degrees Celsius or less, and a mixed solution of safflower extract and citric acid mixed with 0.5% -30% citric acid. Get

7. The safflower extract solution selected from the alkaline safflower extract solution or the citric acid safflower extract solution or the mixed solution of the alkaline safflower extract and the citric acid safflower extract solution was heated to 70 degrees Celsius, and the carrier composition of stearic acid 100g-450g, glycerin 100g-450g . 10g-30g of fiber, 4g-14g of Ar-165. Lanette 1665 weighs 10g-40g. GMS-105 6g-14g weight. GMS-205 6g-14g weight. D-P 1.6g-6g weight. Tween60 10g-30g weight. LP # 70 20g-200gweight. IPM 20g-120g weight. SILICONE200 / 100CS 2g-20g weight. ALLANTOIN 0.2g-1.6gweight. Clofenensin or benzoic acid 10 g-100 g weight. PG 20g-100g weight. 2 g-40 g weight of 1% of hyaluronic acid. Ceramide 2g-100g. 0.5-8 g of collagen, 0.5 g-8 g of corndracin, 0.5 g-8 g of elastin. CARBPOL 941 2g-50g weight. MSM 40g-100g weight. Sulfur 0.3g-20g Aloe extract 1g-100g parts by weight, alum 2g-40g parts by weight. Borax 2g-40g parts by weight. 1g-100 parts by weight of rosin. Pearl powder 3-40g parts by weight, talc 4-40g parts by weight, gypsum 4-40g parts by weight, baekbaek 1g-100g parts by weight, yellow white 1g-100g parts by weight, Honggyeongcheon 1g-100g parts by weight, white paper 1g-100g parts by weight, White ginseng skin 1g-100g weight part, white skin 1g-100g weight part. 1g-50g parts of cholesterol, 1g-100g parts of camphor. Silver was selected from 0.001g-2g parts by weight of at least one of the substances added, and then cooled in a container to prepare a skin external preparation comprising the safflower of the present invention.

Example 2.

Extraction of the active material of the addition.

1. Put 3 g-10 g of potassium hydroxide and 3 g-10 g of sulfur in 1000 ml of the solution or purified water obtained in the above step 1-4 into a container and liquefy. At this time, select one of platinum, palladium, and rhodium-plated precious metals on one of the +-electrodes of the wire through which electricity can flow, and attach the stainless steel to the other electrode and install it in a container. The -25 volt electricity can be dissolved by drawing in 2 seconds-10 seconds and stirring.

When the sulfur solution is mixed with a carrier prepared in Example 1, the carrier 100 is mixed with 0.03 g-20 g parts by weight.

2. Prepare a solution by adding 1 g-10 g of potassium hydroxide or potassium iodide and 1000 ml of purified water to a container, or by heating only with purified water to extract an active substance to obtain an aqueous solution of the active substance, and divide each 200 ml into three containers of the solution. The solution is extracted by impregnating 0.2g-60g of each selected material from herbs such as baekbaekpi, baekbaek, honggyeongcheon, baekji, aloe, baeksunpi, baekbuja in each solution.

3. In the preparation of the carrier according to Example 1, 0.1% -20% of the extract solution of each substance is mixed with the total amount of carrier 100 in the preparation process of the carrier.

Example 3.

0.3 g-15 weight part of rucinols in 100 g of the composition obtained by the process of Example 1 or the process of Example 2. 0.3 g-15 g weight of terane. Cogine-Q10 0.2g-5g parts by weight of any one or more selected by further mixing and heated to a temperature of 60 degrees Celsius-70 degrees Celsius 5 minutes-60 minutes to obtain a skin external preparation containing sulfur, safflower extract .

Example 4.

The composition for external application of skin is further included in 100 g of the composition obtained in Example 1 and Example 1-7 by further adding 0.05 g-5 g of a substance selected from at least one selected from vitamin C, vitamin E, vitamin A, and vitamin B6.

Example 5.

Experiment 1.

Example 4: Cyclooxygenase Activity Test

2-1: Cyclooxygenase-2 Inhibitory Effect

Experiments were conducted to know the effect on the activity of cyclooxygenase-2 in the composition prepared by the present invention.

RAW 264 · 7 of the amount of cultured cells with the addition of ribonucleotides, and Chi method replicon saccharide and then after 24 hours cyclooxygenase -2 Gras Dean E ₂ is water arachidonic acid metabolism that are generated by prostasin (cyclooxygenase-2) Quantitative ELISA method (Caman) is shown in Table 1 below.

In addition, to measure the direct inhibitory ability of cyclooxygenase-2 (cyclooxygenase-2), after washing for 24 hours after treatment with ribopli saccharite prepared by the achidonic acid (archidonic acid) and Example 1-Example 2 The composition was measured 30 minutes after each addition. The results were repeated three times and listed in Table 1 (Biochem Pharmacol. 61. 1195-1203 <2001>).

Table 1 Inhibitory Effect of Prostaglandin E ₂ Production in RAW264 · 7 Cells

As can be seen in Table 1, the composition of the present invention was found to inhibit the production of prostaglandin E ₂ at a concentration of 25 μg / ml without cytotoxicity.

As shown in the results of the 30-minute experiment, it was found that they directly inhibit cyclooxygenase-2.

2-2: Cyclooxygenase-2 Inhibitory Effect

First, 1,100 μM arachidonic acid and 25 μg hemoglobin were added to Tris buffer solution (0.1 M. pH 8.0) to make a substrate, and 10,000 units of cyclooxygenase and SBC, SBCO prepared according to Examples 1 and 2 were prepared. -1 and SBCO-2 are added at different concentrations. The cyclooxygenase activity of the enzyme was measured by the initial spring consumption (Yellow Spring Intrument Company.Model 53oxygen monitor) and the results are shown in Table 2 below. .

TABLE 2

* N = 3

As can be seen in Table 2, the skin disease improvement cosmetic composition of the present invention was able to confirm that the inhibitory effect on cyclooxygenase is excellent.

Example 5: Anti-inflammatory Effect Experiment

In order to determine the anti-inflammatory effects of the compositions SBC, SBCO-1, SBCO-2, the evaluation method using arachidonic acid (A. Crummey, GP Harper, EA Boyle and FR Mangan. Inhibition of arachidonic acid-induced ear edema as a model for assessing topical anti-inflammatory compound.Agents and Actions 1987: 20: 69-76).

Each of the 35 hairless mice, 7 of each, was cleaned with ethanol before sample application, and measured using a micrometer, and then treated with 0.5% and 1.0% of the composition of the present invention and indomethacin. (indomethacin) was divided into a comparison group treated with 1.0% and a control group treated with only arachidonic acid to conduct an experiment to determine the average value of the ear thickness of the mouse. 20 µl of the sample and 20 µl of ethanol were continuously applied to the control group for the treatment of the composition of the present invention and the comparison group for the treatment of indomethacin for 4 hours once a day.

One hour after the last application, ethanol was applied to the left and arachidonic acid was applied to the right ear to 2 mg / ear. After 1 hour of application, the edema of the ear was repeated three times for both ears using a micrometer. Measured. The left ear of the treated group was used as a control to determine the degree of inflammation caused by the sample itself. The anti-inflammatory effect was determined in the degree of edema inhibition based on the control group treated with arachidonic acid only, and the results are shown in Table 3.

TABLE 3

% Inhibition = (A-B) / A x 100

A: average thickness change of control ears (thickness of arachidonic acid treated-thickness of untreated ears)

B: Thickness of sample coated group ears-thickness of untreated ears (thickness of sample treated ears-thickness of untreated ears)

As shown in Table 3, the 1.0% SBC and SBCO 1.0% samples of the present invention showed high inhibition rates of 59.2% and 61.3%, respectively. These figures were shown to have a better anti-inflammatory effect than Indomethacin, which is a very good anti-inflammatory substance as a pharmaceutical ingredient. In addition, SBC, SBCO-1, and SBCO-2 showed a significant difference than that of arachidonic acid and indomethacin.

Example 6: Antimicrobial Activity

4-1: Antimicrobial Activity against Gram-positive Cocci

Staphylococcus intermediusrbs strain of atopic dermatitis was used in this experiment. This strain is the most commonly isolated strain from pyoderma and is the causative agent of superficial and deep pyoderma. Therefore, to determine the effect of the present composition on Staphylococcus aureus, the main cause of infection of skin diseases, the strains were tested as shown in Table 4 below.

1) Use strain: Staphylococcus intermediusrbs isolated from eczema ringworm skin, a kind of atopic dermatitis.

2) Experimental Method: After clotting 2 clony of Staphylococcus bacterium cultured in blood medium in 30ml physiological saline, it was diluted again 1000 times and used.

Negative Control: 9.99 ml saline and 10 ul of bacterial suspension.

Positive control: 9.99 ml of saline solution (cephalexin: final concentration -100 mg / ml) and 10 ul of bacterial suspension

Treatment groups: 2 mg of SBC, SBCO-1, SBCO-2 and 10 ul of bacterial suspension.

The reaction was carried out as above. The bacterial colonies of each group were measured at intervals of 10 minutes, 30 minutes, and 1 hour after the reaction (plate method).

3) Results: In the treatment of the present invention, as shown in (Table 4), the growth of bacteria was suppressed 100% in all of 10 minutes, 30 minutes, and 60 minutes.

[Table 4] Experimental method

TABLE 5

Comparison of Antimicrobial Activity against Gram-positive Cocci of the Compositions among Experimental Groups

As shown in Table 5, the growth of Staphylococcus aureus was confirmed in the negative control group because it did not contain antimicrobial agents. In the group using cephalexin as a positive control group, the growth of bacteria was observed in 10 minutes, 30 minutes, and 60 minutes. 100% inhibition. Accordingly, the present compositions SBC, SBCO-1, SBCO-2 proved to have an antimicrobial activity against Gram-positive cocci, particularly Staphylococcus aureus.

4-2: antimicrobial activity against gram-negative bacilli

Escherichia coli (E. coli) strain isolated from the urine of dogs infected with urinary infection was identified and used in this experiment.

1) Use strain: Escherichia coli (E. coli) as E. coli.

2) Experimental Method: Two colonies of E. coli bacteria cultured in blood medium were suspended in 30 ml saline solution and diluted 1,000 times.

Negative Control: 9.99 ml saline and 10 ul of bacterial suspension.

Positive control: 9.99 ml of saline (sephalexin: final concentration -100 mg / ml) and 10 ul of bacterial suspension.

Treatment groups: 2 mg of SBC, SBCO-1, SBCO-2 and 10 ul of bacterial suspension.

The reaction was carried out as described above, and the bacterial colonies of each group were measured at intervals of 15 minutes, 30 minutes, and 60 minutes (plate method).

Table 6 Experimental Method

Table 7 Antimicrobial Activity Test Results

As shown in Table 7, the growth of Escherichia coli was confirmed in the negative control group because it did not contain the antimicrobial agent. In the group using cephalexin as the positive control group, the growth of bacteria was increased in all 10, 30, and 60 minutes. % Was suppressed. Accordingly, the composition has been demonstrated antimicrobial activity against Gram-positive cocci, particularly E. coli.

4-3: Antimicrobial Effect of Staphylococcus aureus

Based on BHI (Brain Heart Infusion) medium using Staphylococcus aureus (ATCC (American Type Culture Collection) 6538), one of the causative agents of atopic dermatitis, as shown in Table 8 below. After culturing in the same medium, the number of viable cells was measured over time (UV spectrometer, 420 nm wavelength), and the results are shown in Table 8 below. Dilution of the medium for measuring the optical density (O.D) for viable cell count was diluted 10-fold with saline. The control bacterium used neomycin.

[Table 8] Absorbance over time of incubation

A: SBCO-2 0.8g / 100ml Medium

B: neomyxin 0.01g / 100ml medium

C: SBC 0.8g / 100ml Medium

D: polymixin 0.01g / 100ml badge

E: BHI media

The medium used in Table 8 is an example of using the amount of SBC as 0.8g in 100ml of the medium.

According to the results of Table 8, there was no growth of bacteria in the SBC-containing A and C medium for up to 27 hours, and in the B medium containing neomyxin up to 12 hours, using D medium or BHI medium itself. It can be seen that the growth rate of the bacteria is significantly reduced compared to the E medium. In particular, it can be seen that the growth inhibition of the bacteria is greater in A medium using sbco-2.

4-4: Confirm antimicrobial effect against acne-inducing bacteria

Propylonibacterium acne known as acne-inducing bacteria (Spion: Propionibacterium acne, KCTC (Korean Collection for Type Cultures, Bacterial Bank of Biotechnology Resource Center of Korea Research Institute of Bioscience and Biotechnology) 3314) was used, and Wilkins Chalgren Anaerobe Broth Incubated in a medium adjusted as shown in Table 3 on the basis of the following, and measuring the viable cell number change over time (UV spectrometer, 420 nm wavelength), the results are shown in

Indicated.

[Table 9] Absorbance over time of incubation

A: 0.8 g / 100 ml medium + 1.85 g trisodium phosphate

B: Polymixin 0.8 g / 100ml Medium

C: SBCO-2 0.8 g / 100 ml medium

D: Neomyxin 0.08g / 100ml Medium

E: medium control

As shown in Table 9, it can be confirmed that there is a high bacteriostatic (or bactericidal) effect in A and C medium using SBC.

Example 7: Animal experiment on the treatment effect of skin disease

7-1 Experiment Animals

The experimental animals were supplied with two dogs (dog doberman) treated at the animal hospital with atopic eczema, filamentous fungal infection and ringworm, and solid feed manufactured by a famous feed company until the day of the experiment. After supplying enough water and water, it was used for 3 days in an environment of 22 ± 2 degrees Celsius and 55 ± 15% humidity.

Clinical pathological findings of laboratory animals were 5.3 cm in diameter in the lower left neck. Width 3.2cm. Depth 5 mm. 7.2cm long on the neck of the lower right ear. 5.2cm wide. 6 mm deep, damaged parts of the dermis and subcutaneous tissue, 2-3 mm keratinocytes on one side of the epithelium, and severe wounds with slight inflammatory findings on the damaged cells were visually observed. In five sites, the alopecia of alopecia with a diameter of 2cm-8cm, centrifugal dilatation of the erythema and white keratin were removed from the area, and the necrosis progressed.

In each of the damaged areas, the blister membrane, the skin, the tissue, the mortar, and the like, were taken on the slide glass by a 3-5 mm incision, and 25% of potassium hydroxide was dropped and covered with a cover glass. After microscopic observation, it was proved that they are ringworm and filamentous fungi.

Electron microscopic findings indicate that the disease of the neck is atopic eczema and anti-vesicle ringworm, which is caused by ringworm, and the posterior thigh from the chest is a fungal infection caused by filamentous fungus and a complex skin disease caused by pancreatic ringworm.

7-2: Sample

The skin disease treatment functional cosmetics SBC, SBC) -1 and SBCO-2 prepared according to the embodiment of the present invention were used as samples.

The control group of this experiment was selected as one of the left and right sides of the neck and two of the five necrotic skin parts using a test material having the same biochemical values of body fluids. For the control group, the right neck wound area, right ringworm and fungal infection site were selected.

7-3: Clinical treatment of the lesion site

After controlling the behavior of the test animals, alcohol disinfection of the left wound and the surrounding area, all the surrounding hairs were removed, and after disinfection, local anesthesia was performed using 2% lidocaine HCL, and the abscess was washed with gauze and hydrogen peroxide. After removing the keratin in contact with the skin including the dermis using a mass, spray the alcohol on the damaged wounds using a spray nozzle, dry with air, and mix SBC, SBCO-1, and SBCO-2 in three portions. The wound was then protected with gauze and a neck guard was placed to prevent access to the hind legs. Necrotic skin has 2-3mm keratin. After removing the hair in the same manner as the above treatment, rubbing until the thickness of the keratin thinner using a diamond paper, rubbing the alcohol with a disinfectant, and then dried, gently tapping with the SBC mixed composition into the skin After infiltrating the active ingredient of SBCO-2, it was once again treated with SBCO-2 and treated three times daily.

In the clinical treatment of the control group, wounds with atopic eczema in the neck area of the control group were treated with conventional treatment methods, such as teramicin ointment or neomycin ointment, or ringworm therapeutic agent after treatment with exfoliation and exfoliation. Three times daily application of antibiotics, such as griseofulvin, ointments from steroids, antihistamines, and immunosuppressive agents such as centellasiatica and elidel for rapid recovery of wounds, were treated two times. The same treatment as for the neck was performed.

7-4: Kill Results

Compared with the control group, the treatment result is as follows.

a) observation of control group

(The third day of the treatment)

Visual observation of the treatment site showed that the treated wound in the right side of the neck showed a small amount of subcutaneous bleeding, a small amount of inflammation, a slight contraction of the wound, and necrosis from the right chest to the thigh. In the ongoing two skin areas, several small blisters are observed on the skin surface of the erythema. The eccentric expansion of the erythema stops, and in the action, the hind foot is in contact with the neck and the wall is in the right area. We can see that the number of rubbing actions decreases.

(The tenth day of the treatment)

Visual observation of the treatment site showed that the bleeding in the upper right area of the neck was stopped, the wound was reduced to 6.8cm in length, 4.5cm in width and 5mm in depth, and the infection site of fungus and ringworm was changed to light red. It became.

(The 30th day of the treatment)

The wound depth of the neck was constricted, but the skin of the wound was dried, so that a fractured crack was observed, and the color of the skin was changed to a reddish red color and the wound was deteriorated again. Fungal and ringworm infections were not changed.

b) Observation of the composition treatment group of the present invention

The treatment group treated with the composition SBC, SBCO-1, SBCO-2 prepared according to the present invention in combination was observed.

(The third day of the treatment)

No bleeding or secretion of the neck was observed, no inflammation was observed, the color of the wound tissue was pale pink, and the formation of blood vessels was well progressed for the restoration of cells from the subcutaneous tissue visually. It has been confirmed that the production of collagen is actively progressing. Also, in the ringworm and fungal dermatitis, no thick keratin remodeling was observed, but the color of the skin was changed to light orange.

(The tenth day of the treatment)

The wound was reduced to 2.6 cm in length, 2 cm in width, and 2.6 mm in depth, did not develop inflammation upon observation of inflammation, and scratching of the wound with the hind paw was not observed. The color of the stratum corneum of the ringworm and fungal infection area was changed to white, and the centrifugal area was reduced.

(The 30th day of the treatment)

The neck wound was the same color as normal skin, and the wound was healed into normal skin without dryness, and the skin of the fungus and ringworm infected area was confirmed that the erythema disappeared and the fine hairs were protruding. Relapse could not be confirmed by the 50th day of treatment.

c) Comparison of therapeutic functionalities between control and sample treatment groups by measuring IgE concentrations.

On the 30th day of the end point of application of the sample, 100 μl of the control group and the sample treatment group were soaked using capillary tubes in the skin of the control group and the sample administration group, and then centrifuged at 6,500 rpm for 20 minutes, and then 30 μl of serum Were isolated and serum IgE was measured using an ELISA kit (R & D system).

TABLE 10

According to the skin treatment example, it is effective in the treatment of filamentous fungal infections, and it is effective in restoring the epidermis, including the subcutaneous tissue, and the damage of the dermis. As shown in the table, the control group can be seen in atopic dermatitis. The concentration of IgE serum, which is an ataxia of the immune system, is increased. And because the sample treatment group is close to normal, it is effective in treating atopic dermatitis, eczema and dry skin.

In addition, the possibility of treatment for intractable skin diseases caused by immune system abnormalities such as psoriasis and palmar sinusitis, which are diseases caused by abnormal autoimmune system involvement in skin tissue immune system, was confirmed.

Example 8 Clinical Trial Results for the Treatment of Skin Diseases

Atopic dermatitis, dry ringworm and foot ringworm. Antiphasic vesicle ringworm. Various eczema. Malignant acne (acne). Itching of the skin. Shingles. Psoriasis, chronic skin conditions. Purulent diseases, such as palmar ulcer vesicles. Ten of each athlete's foot athletes apply SBC ointment to the skin disease area to do a pat and rub massage.

Shingles

Apply to wounds twice daily morning and afternoon. The application was applied twice or more times a day for six days. The improvement effect among those with skin of the disease is the effect of partial improvement of two people. Nearly perfect cure 8 people.

3. Atopic Dermatitis

Clinical example 1

Seven people with chronic atopic disease skin was massaged by applying the composition of the present invention three times a day. In 5 patients, there was a remarkable relief effect from 3 to 5 days, complete improvement effect from 10 days, complete regeneration of damaged skin after 30 days, pruritus disappeared from the next day of application, and 2 people started to relieve from 26 days after 3 months The result is a complete improvement effect.

Clinical example 2

Three people with early atopic disease skin were applied twice daily and massaged. Itching disappeared immediately after the application of the massage, three times a day after 6 days, twice a day after 10 days, massage was stopped after 60 days and completely improved.

Clinical Example 3.

The composition of the present invention was applied twice a day to two children's skins of the third year of atopic disease and massaged. The massage did not immediately feel itching, the erythema disappeared after 2 days of massage and the application massage was stopped due to a complete improvement effect at 60 days.

Clinical Example 4.

A skin external preparation prepared according to Example 1-5 of the present invention is applied to the outer surface of the skin of 15-year-old children aged 3 to 17 years old who have been diagnosed with atopic dermatitis by a dermatologist and have a history of severe atopic dermatitis. Apply three times a day to treat and observe.

Result: Pruritus disappears after 3-5 minutes of application.

Clinical example 5

Five patients diagnosed with pruritus on the skin around the anus were coated with the composition obtained in Examples 1-8 and the symptoms were observed. Pruritus improved immediately after application.

Whitening Functional Experiment.

The facial skin was treated with twice daily application to the facial skin of 38, 52, and 41 year old women with pale gray stains, and the results were observed.

RESULTS: After 30 days, the skin of all three subjects changed to milky skin color, and the composition of the present invention was found to have a whitening effect.

.

Claims (13)

A topical skin composition comprising safflower extract and a pharmaceutically or cosmetically acceptable carrier. According to claim 1, The skin external composition comprising safflower extract is 0.1 to 20 parts by weight based on 100 parts by weight of the carrier. According to claim 1, wherein the topical skin composition is based on 100 parts by weight of the carrier 0.03 to 20 parts by weight of sulfur. Aloe 0.1 to 20 parts by weight. 0.1 to 20 parts by weight of lettuce. Yellowish white 0.1 to 20 parts by weight. 0.1 to 20 parts by weight of white paper. 0.1-20 parts by weight. Baekjaja 0.1 to 20 parts by weight, ringworm skin 0.1 to 20 parts by weight. Alum 0.1 to 10 parts by weight. Rosin 0.1 to 10 parts by weight. 0.1 to 10 parts by weight of pearl powder. 0.1 to 10 parts by weight of cholesterol. Talc 0.1 to 10 parts by weight. Camphor 0.1 to 10 parts by weight, gypsum 0.1 to 10 parts by weight. Borax 0.1 to 10 parts by weight. The skin external composition comprising a safflower extract is configured by adding at least one selected from the group consisting of 0.001 to 2 parts by weight. According to any one of claims 1 to 3, wherein the external preparation composition for skin further comprises 0.1 to 30 parts by weight of plant extracts or plant essential oils based on 100 parts by weight of the carrier, wherein the plant or plant essential oils are 300 seconds, stain , Honeysuckle, horsetail, chickweed, comfrey, persimmon leaf, rice bran, mulberry, cocoon, eggplant. Rat melon. Tenza. apricot. Peach leaf or doin. Dong-ah. Pomegranate. Sidelines. Acacia, Schizandra. Plantain. Bear smell. Centennial, Pogongyoung. Gold. Kwonbaek. Brotherhood. goldthread. Gold. frankincense. Gyeji, Angelica, Machi, Rhubarb, Ginkgo, Garlic, Carrot, Yulmu, Centella, Green onion, Grapefruit, Perennial, Injin, Green tea, Rose of sharon, Tempered chrysanthemum, Hoi cho, Cactus, Ratberry, Chrysanthemum, Chestnut, Tare, Ginseng Willow , Evening Primrose, Lime tree, Centella asiatica, Seaweed, Licorice, Harpoon, Herb, Peony, Lily, Yeongneung, Cypress, Burdock, Apple, Grapes, Fruits, Mushrooms, Legumes, Sasam, Lee, Platinum , Lining tree, niglospores perica, marsh tree, carnation, collarde cavallo, beauties, walnut caridica, astaxanthin, acerola spicy, starfish, pine, birch, chemomile, marigold, rosemary, peppermint With lemon balm, Douglas, jasmine, propolis, allantoin, tea tree and lavender At least one skin external preparation composition selected from the group consisting of. The skin external composition according to any one of claims 1 to 4, wherein the plant extract is a solvent extract obtained by extracting the plant with one or more solvents selected from the group consisting of water, an aqueous alkali solution and an alcohol of C1-C4, or a plant essential oil. . According to any one of claims 1 to 4, wherein the external application composition for skin is flufenamic acid, ibuprofen, benzydamin, indomethacin, prednisolone , And dexamethasone (dexamethasone) further comprises at least one anti-inflammatory agent selected from the group consisting of 0.0001-2.0 parts by weight based on 100 parts by weight of the carrier. The method of any one of claims 1 to 4, wherein the topical skin composition is Aminoglycosides, Glycopeptides, Tetracyclines, Spectinomyxin, Bacitracin. Polymyxin, and Fosfomyxin further comprises at least one antibiotic selected from the group consisting of 0.0001-2.0 parts by weight based on 100 parts by weight of the carrier. The external preparation composition for skin according to any one of claims 1 to 4, wherein the composition is in liquid, latex, gel, cream, paste, or sheet form. The composition for external application for skin according to any one of claims 1 to 4, wherein the composition is for improving or treating a skin disease. 10. The composition for applying the external of the skin according to claim 9, wherein the skin disease is dermatitis, acne, dry skin, athlete's foot, eczema, allergic urticaria, pruritus, ringworm, psoriasis, varicella effusion, or atopic disease. The external preparation composition for skin according to any one of claims 1 to 4, wherein the external preparation composition for skin is a cosmetic composition. 12. The composition of claim 11 wherein the composition is a skin gel, cream, lotion, powder, foundation, essence, gel, pack, foam cleansing, soap, skin ointment. A skin external preparation composition that is a soap, cleansing, oil, powder foundation, emulsion foundation, or wax foundation formulation. The composition of claim 11, wherein the composition further comprises at least one additive selected from the group consisting of vitamins, amino acids, proteins, surfactants, emulsifiers, stabilizers, thickeners, preservatives, humectants, antioxidants, flavorings, pigments, stabilizers and preservatives. The external preparation composition for skin containing.

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