KR20110130184A - An extract of cudrania tricuspidata and a cosmetic composition containing the extract with anti-acne effect - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Cudrania tricuspidata BUREAU extract is provided to suppress the growth of Propionibacterium acnes without side effects. CONSTITUTION: A Cudrania tricuspidata BUREAU extract for preventing and treating acne is prepared using water, hydrophilic organic solvent, or mixture solvent thereof, precipitating for a certain time, stirring, and filtering. The extraction solvent is anhydrous or hydrous methanol of 1-4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol, or butylenes glycol.

Description

An extract of Cudrania tricuspidata and a cosmetic composition containing the extract with anti-acne effect

The present invention relates to a method for extracting Cucumber tree and a cosmetic composition for acne skin containing the same, and more particularly, there is no irritation and safety on the skin and there is no problem of causing skin diseases, and by preventing the growth of acne-inducing bacteria, acne prevention and improvement It relates to a cosmetic composition for acne skin comprising a / relaxing effect.

The development of acne can lead to active sebum production due to changes in hormone secretion in the body, as well as genetic and environmental factors, closure of pores due to abnormal hyperkeratinization of hair follicle sebaceous glands, activation of acne bacteria and skin inflammatory reactions caused by acne bacteria. Four major causes and their interactions.

Therefore, acne from anti-androgens that inhibit sebum production, non-steroidal anti-inflammatory drugs that act as anti-inflammatory agents, resorcinol, sulfur and benzoyl peroxides that have antibacterial effects, and tetracycline, erythromycin, clindamycin, and mercuride that have antibiotic effects Methods of inhibiting fungal activity and treatment using vitamin A derivatives such as retinoic acid are used as acne treatment methods.

However, these conventional acne treatment methods have a number of problems in terms of efficacy or skin safety. The use of hormonal agents has been reported to cause adverse effects such as growth inhibition of the epidermis and hormonal abuse. Retinoic acid and benzoyl peroxide may cause birth defects, skin irritation, contact dermatitis, and clindamycin. Antibiotic of has been reported to have side effects such as emergence of resistant bacteria, allergic reactions, photosensitivity reactions.

On the other hand, acne treatment is not a short-term, but a steady and continuous treatment is required, so the acne treatment method with the above problems was only available for a short time or a limited use.

In recent years, researches are actively underway to search for effective ingredients that reduce the side effects from natural resources, including plants, and can be continuously used, and have a powerful anti-acne bactericidal effect. In Korea, extracts of situational mushrooms, green tea and golden, willow, licorice and licorice are used as raw materials for functional cosmetics and soaps related to acne treatment, and the development of cosmetic raw materials with anti-acne bactericidal efficacy using chemicals derived from natural products It's going on. However, the effect is very weak compared to the conventional acne treatment material, there is a problem that the mass production of active substances related to acne treatment is difficult.

In particular, natural products are composed of various components, and the method of separating and purifying effective active substances depends on the physicochemical properties of the active substances, and among the methods of separation and purification based on several different principles, it is possible to effectively combine appropriate methods. Since it has to be used, there are a lot of difficulties in extracting and separating substances having anti-acne activity from natural products.

Cucumber tree is a deciduous broad-leaved small arborescent tree of the dicotyledonous plant, Nettle, Mulberry, and is distributed in Korea (Jeonnam, Jeonbuk, Gyeongnam, Gyeongbuk, Chungnam, Yellow Sea), Japan, China, etc., and grows on both sides of villages and near villages. The main components of Cucumber tree are flavonoids, morin, rutin, beta-sitosterol, kaempferol-7-glucoside and populnin. ), Proline, glutamic acid, arginine, arginine, asparaginic acid, glucoside, glucoside, statachydrine, atocafesin, norarthocarpetin, 5 5-O-methylgenistein, carbohydrate (sucrose, glucose, organic acids), and the like. Leaves and berries are edible, and in one shot, stems and leaves are used as medicinal herbs. It has been reported to be effective in relieving pain, eliminating inflammation, and strengthening muscles and bones, thereby treating neuralgia, arthritis, boils, swelling, dermatitis, eczema, and bruises.

However, there have been no studies on the use of acne bacteria in acne bacteria, and there have been no reports on the use of acne prevention and / or treatment. The technique used has not been disclosed.

The present invention solves the problems of the prior art as described above is hypoallergenic and has no problem in causing skin diseases and excellent antimicrobial efficacy with respect to acne-inducing bacteria to provide a cosmetic composition for acne skin showing an acne prevention and improvement / alleviation effect The purpose.

In order to achieve the above object, the present invention is hypoallergenic, including the method of extracting the acne-improving active ingredient from Kudji mulberry tree and the active ingredient obtained therefrom, there is no problem in inducing skin diseases and has excellent antibacterial effect on acne-inducing bacteria. It provides a cosmetic composition for acne skin exhibiting an acne prevention and improvement / alleviation effect.

Extract of Cudrania japonica of the present invention is the leaching, elution from the Cudrania japonica, or some or all of the leaching liquor again, or prepared by drying the concentrate again, acupuncture, premises, regular, flow extract, etc. It is a concept that includes the chemicals that are contained in them and have a major effect.

Preferred Cucumber extract of the present invention is to add a predetermined extraction solvent to Cucumber tree, extracting a component that inhibits the growth of or propionibacterium acnes (Propionibacterium acnes) that exhibits acne prevention and / or therapeutic effect from the Cucumber tree solid extract ( It can be the primary extract of Cudrania japonica obtained by solid-liquid extraction.

The Cudrania tree of the present invention is prepared by completely drying after crushing, the Cudrania extract of the present invention by adding a hydrophilic organic solvent, such as methanol, ethanol, or a mixed solution of hydrophilic organic solvent and water to the Cudrania tree as an extraction solvent It can be obtained by the usual natural product extraction method of filtering and recovering the solution obtained after stirring at an appropriate time interval while depositing time.

Preferred extraction solvents include anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol, a pure solvent selected from the group of solvents including water or a mixture of two or more thereof A solvent may be used and the amount of the extraction solvent added to the plant material may be about 1 to 50 times the total volume of the plant material.

More preferably, as the extraction solvent of the extract of Cudrania japonica of the present invention, one pure solvent selected from anhydrous methanol, hydrous methanol, anhydrous ethanol and hydrous ethanol or a mixed solvent of one of the methanol and one of the ethanol is used. This is preferred.

At this time, the addition amount of the extraction solvent is preferably set to 5 ~ 20ml per 1g dry weight of Koji. Extraction may be carried out by a conventional general solvent extraction method such as extracting by immersion 1-7 days at room temperature or 1-5 hours at 50 ~ 95 ℃ heating conditions.

The cosmetic composition for acne skin of the present invention is characterized in that it comprises an active ingredient which has a prophylactic and / or therapeutic effect derived from the extract of Cudrania japonica, and the content of the Cudrania japonica extract is preferably 0.001 to 0.1% by weight. Do. This may cause side effects such as skin irritation and toxicity at high concentrations of more than 0.1% by weight, because the effect is weak at low concentrations of less than 0.001% by weight.

The cosmetic composition of the present invention is, in addition to the extract of Cucumber japonica, if necessary, various components generally used in cosmetics within the range of not deteriorating the effect of the present invention, for example, water-soluble components, powder components, oil, surfactants. , A moisturizer, a viscosity modifier, a preservative, an antioxidant, a flavoring agent, and a pigment can be added.

The cosmetic composition containing the extract of the Quercus japonica extract of the present invention may be prepared in any formulation commonly prepared in the cosmetic industry, for example, solutions, suspensions, pastes, gels, creams, lotions, powders, soaps, oils. It may be formulated as powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it is prepared in the form of a flexible lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, latex foundation, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, soap or powder Can be.

Cudrania japonica extract of the present invention can be used as an external preparation for acne skin in addition to the cosmetic composition for acne skin. Hereinafter, the present invention will be described in detail with reference to more specific examples, but the present invention is not limited only to the following examples.

The present invention solves the problems of the prior art as described above is hypoallergenic and there is no problem in inducing skin diseases, and excellent in antibacterial effects on acne-inducing bacteria to form a cosmetic composition for acne skin to show acne prevention and improvement / alleviation effect It can be used by adding to all forms of the stowage ensured the stability of.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

1. Use strain, medium and culture conditions

(1) strain

The strain used in the present invention is Propionibacterium which is the cause of acne acnes KCTC 3314, these strains were used by the Korea Gene Bank.

(2) medium and culture conditions

Reinforced clostridial (RC) medium (Merck, Germany) was used as a culture medium for acne bacteria, and P. acnes was activated 3 days before the experiment while stored at 4 ° C. The bacteria were inoculated in the culture medium and sealed in an anaerobic jar using a Gaspack system (Merck AnaerocultGaspack system, Germany), followed by anaerobic culture for 3 days in an incubator at 37 ℃.

Antimicrobial activity was measured using paper disk diffusion method and Broth dilution assay.

Example 1. Preparation of Cudrania extract

Cuji mulberry was purchased from commercially available dried outpost to prepare a powder using a grinder, and then put into 500g Cuji mulberry powder 10% 95% ethanol, and extracted with stirring at room temperature for 3 hours. Then, the extract solution was collected by filtration using a filter paper, and the recovered solution was concentrated under reduced pressure at 47 ° C. using a distillation apparatus equipped with a cooling capacitor.

Test Example 1 paper disk diffusion assay

After culturing the strain in a Reinforced clostridial (RC) liquid medium for 3 days, the bacteria suspension was adjusted to a constant concentration and evenly spread on a RC agar plate using a sterile swab. After the plate was dried, paper disk (Φ 8 mm, Adventec. USA) was placed on the plate surface inoculated with the strain, and the Cudrania extract was diluted in DMSO by concentration to absorb 50 μl / disk on the paper disk. The plate treated with the sample was incubated in an anaerobic jar using a Gaspack system for 3 days, and then the antimicrobial activity was measured by the size of the inhibition zone around the disc.

At this time, as a control, triclosan (triclosan), which is widely used as an antibacterial material, was used in the cosmetic composition.

Size of Acne Bacteria Growth Inhibitory Ring of Cucumber Mulberry Extract Sample Name Treatment concentration Restraining ring (mm) Triclosan 156 μg / ml 25 ± 2 313 ㎍ / ml 30 ± 2 625 μg / ml 36 ± 2 1250 μg / ml 38 ± 2 2500 μg / ml 46 ± 2 5000 μg / ml 48 ± 2 10000 μg / ml 50 ± 2 Example 1 1250 μg / ml 13 ± 2 2500 μg / ml 16 ± 2 5000 μg / ml 18 ± 2 10000 μg / ml 19 ± 2 50000µg / ml 22 ± 2

From the results of Table 1, the triclosan has a growth inhibitory ring size of about 50 ± 2 mm at a concentration of 10000 ㎍ / ml, and the 95% ethanol extract of Cudrania japonica is about 19 ± 2 mm at a concentration of 10000 μg / ml. Confirmed. However, this is a high potency concentration compared to triclosan as a single component, but considering that the zigzag 95% ethanol extract is a crude extract (crude extract) contains an excellent anti-acne fungal efficacy component.

Test Example 2 Broth dilution assay

Liquid medium dilution was performed by partially modifying broth-dilution using 96-well plates (K. Yasunaka et al., Antibacterial activity of crude extracts from mexican medicinal plants and purified coumarins and xanthones, J. Ethnopharmacol., 97, 293 (2005)). The acne bacteria cultured for 72 hours were adjusted to the value of McFarland standard 0.5, and 100 µl of the strain diluted to 1 × 10 6 CFU / ml was inoculated into each well containing 100 µl of the medium. The 95% ethanol extract of Cucumis japonica was dissolved in DMSO, and 100 μl each was added to the 96-well plate containing the strain and the medium using RC liquid medium and mixed well. After incubating 96-well plates inoculated with bacterial dilutions and samples for 3 days using an anaerocult gaspack system, the minimum concentration at which bacterial growth was not determined was determined as MIC (Minimum inhibitory concentration). Triclosan was used as a control.

Minimum Inhibitory Concentration of Acne Bacteria (CIC) Sample Name Minimum Inhibition Concentration (MIC) Triclosan 0.449 ± 0.2 μg / ml Example 1 12.73 ± 2.5 μg / ml

As can be seen from the above results, the minimum inhibitory concentrations of 95% ethanol extracts of triclosan and cooji mulberry tree were 0.449 ± 0.2 ㎍ / ml and 12.73 ± 2.5 ㎍ / ml, respectively. It has a higher efficacy concentration than Triclosan, which is a single component, but considering that Cudrania 95% ethanol extract is a crude extract, it contains an excellent anti-acne bacterium efficacy component.

Cosmetic composition characterized in that it comprises acne prevention and improvement / alleviation of active ingredients derived from kkujippong 95% ethanol extract of the present invention can be content of 0.001 ~ 0.1% by weight of kkujippong 95% ethanol extract. At this time, if the content of Koji mulberry extract to 0.002 ~ 0.05% by weight is more preferable.

Test Example 3 Evaluation of Skin Irritation

In order to evaluate the skin irritation induced by the Koji mulberry extract prepared in Example 1, a primary stimulation test and a cumulative stimulation test were performed on a human body through a human patch test.

Twenty healthy men and women were tested according to CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 20 μl of the sample was added dropwise to a Fin chamber, which was then patched on one side of the arm of the human body, which was the test site, and fixed with tape.

In the first skin irritation test, the patch was removed for 24 hours, and then the skin reaction of the test site after 4 hours and 24 hours after removing the patch was determined according to the criteria of Table 1 below, and the results are shown in Table 4 below. .

The Repeated Insult Patch Test (RIPT) is a three-week routine skin exposure followed by a second closed challenge test on new areas two weeks later to detect irritation and allergens on the skin. As a test to observe, 10 μl of a patient without a history of skin disease was added dropwise to the Finn Chamber in Example 1 at a concentration of 10 mg / ml, and then fixed with Scanpor tape inside both arms as test sites. After 24 hours of application, the patch was removed, and a total of 9 repetitive applications were performed on the same site at the same schedule at 24 hour intervals. The degree of skin irritation was evaluated visually based on the primary skin irritation evaluation criteria, and a scoring scale list was prepared.

Primary skin irritation test sample Number of test subjects Judgment result Irritation degree ++ + ± - Test group 20 - - - 20 0 Control group 20 - - - 20 0 (Criteria)
-: No erythema or unusual phenomenon
±: slightly reddish
+: Significantly redder than the surroundings
++: reddens and swells more heavily than the surroundings
(Stimulus calculation formula)
Stimulus = [{(±) Number × 1} + {(+) Number × 2} + {(++) Number × 3}] / Number of Subjects

Skin cumulative stimulation test sample Number of test subjects Induction stage Challenge stage Irritation degree 20 One 2 3 4 5 6 7 8 9 24h 48h 72h Test group 20 - - - - - - - - - - - - 0 Control group 20 - - - - - - - - - - - - 0 (formula)
Stimulus = [{(±) Number × 1} + {(+) Number × 2} + {(++) Number × 3}] / Number of Subjects

As can be seen from the results of Table 3 and Table 4, as a result of evaluating the skin irritation test on the human body, the single and cumulative patch, Cucumber mulberry extract prepared in Example 1 of the present invention does not cause irritation to the skin It was confirmed that the material is safe to apply.

Cosmetic composition containing kkujippong extract of the present invention can be used in a variety of cosmetics and cleansing agents for acne skin, the dosage form can be arbitrarily selected. Products to which the present composition may be added include cosmetics such as creams, milk lotions, foundations, skins, and the like, which are applied to various skins, and cleansing, face washes, soaps, treatments, and essences.

The following shows a specific formulation example of the cosmetic composition containing the kkujippung tree extract of the present invention, the formulation containing the cosmetic composition of the present invention is not limited thereto.

Formulation Example 1 Skin Compounding ingredient Content (% by weight) Example 2 Carboxyvinyl Polymer 3 Butylene glycol 4 Propylene glycol 3 Methylphenylpolysiloxane 0.1 Methyl paraben 0.1 ethyl alcohol 2 Triethanolamine 0.04 EDTA-2Na 0.02 Purified water to 100

Formulation Example 2 Lotion Compounding ingredient Content (% by weight) Example 2 Carboxyvinyl Polymer 4 Ethyl hydrobenzoate 0.1 Liquid paraffin 3 Vitamin E Acetate 0.1 silicon 0.8 Ketyl Alcohol 0.5 Sorbitan monostearate One Polysorbate 60 0.3 Methylsulfonylfetan 0.2 Propylene glycol 2 Butylene glycol 5 Methyl paraben 0.2 Triethanolamine 0.1 EDTA-2Na 0.03 Purified water to 100

Formulation Example 3 Essence Compounding ingredient Content (% by weight) Example 2 Carboxyvinyl Polymer 15 glycerin 6 Propylene glycol 3 Macadamia oil 0.5 Methyl paraben 0.1 ethyl alcohol 2 Triethanolamine 0.15 EDTA-2Na 0.02 Purified water to 100

According to the present invention can be seen using the kkujippung tree to prevent acne and improve / alleviate the effect, it is possible to extract separation to maximize the activity of the active ingredient exhibiting acne prevention and improvement / alleviation effect, according to the present invention Cosmetic composition for acne skin containing Cucumber extract can effectively prevent and improve / alleviate acne by inhibiting the growth and proliferation of propionibacterium acne. In addition, since the custard tree is a safe plant that has been edible for a long time, according to the present invention, there is no side effect compared to the conventional acne treatment method, and it is possible to obtain a cosmetic composition for acne skin having excellent safety while still being used continuously.

Claims (2)

Cucumber extract for preventing and improving acne extracted by adding water, hydrophilic organic solvent or mixed solvent thereof Claim 1
Koji mulberry extract, characterized in that it inhibits the growth of propionibacterium acne