KR100780666B1 - An extract from Dryopteris crassirhizoma for prevention and remedy of an acne and a composition of cosmetic including the extract - Google Patents

Abstract

The present invention is to prevent acne extracted by adding any one solvent selected from water, a hydrophilic organic solvent, a mixed solvent of water and a hydrophilic organic solvent, a mixed solvent of two or more hydrophilic organic solvents ( Dryopteris crassirhizoma ) and It relates to a therapeutic spectral extract and a cosmetic composition for acne skin containing the spectral extract. According to the present invention by inhibiting the growth of propionibacterium acnes , a major pathogen that aggravates acne symptoms using a spectator that is a natural plant, there is no side effect compared to the conventional acne treatment method, and it is possible to use continuously. A cosmetic composition for acne skin having excellent safety can be obtained.

Spectators, Dryopteris crassirhizoma, Acne, Cosmetics

Description

An extract from dryopteris crassirhizoma for prevention and remedy of an acne and a composition of cosmetic including the extract}

The present invention is to prevent acne extracted by adding any one solvent selected from water, a hydrophilic organic solvent, a mixed solvent of water and a hydrophilic organic solvent, a mixed solvent of two or more hydrophilic organic solvents ( Dryopteris crassirhizoma ) and It relates to a therapeutic spectral extract and a cosmetic composition for acne skin containing the spectral extract.

The development of acne, in addition to genetic and environmental factors, leads to active sebum production due to changes in hormone secretion in the body, pore closure due to abnormal hyperkeratinization of hair follicle sebaceous glands, activation of acne bacteria and skin inflammatory reactions caused by acne bacteria. Four major causes and their interactions.

Therefore, acne from anti-androgens that inhibit sebum production, non-steroidal anti-inflammatory drugs that act as anti-inflammatory agents, resorcinol, sulfur and benzoyl peroxides that have antibacterial effects, and tetracycline, erythromycin, clindamycin, and mercuride that have antibiotic effects Methods of inhibiting fungal activity and treatment using vitamin A derivatives such as retinoic acid are used as acne treatment methods.

However, these conventional acne treatment methods have a number of problems in terms of efficacy or skin safety. The use of hormonal agents has been reported to cause adverse effects such as growth inhibition of the epidermis and hormonal abuse. Retinoic acid and benzoyl peroxide may cause birth defects, skin irritation, contact dermatitis, and clindamycin. Antibiotic of has been reported to have side effects such as emergence of resistant bacteria, allergic reactions, photosensitivity reactions.

On the other hand, acne treatment is not a short-term, but a steady and continuous treatment is required, so the acne treatment method with the above problems was only available for a short time or a limited use.

In recent years, researches actively searching for effective ingredients that reduce the side effects from natural resources including plants and can be continuously used, and have a strong anti-acne bacterium effect, are in progress. In Korea, extracts of situational mushrooms, green tea, and golden, willow, licorice and licorice are used as raw materials for functional cosmetics and soaps related to acne treatment. It's going on. However, the effect is very weak compared to the conventional acne treatment material, there is a problem that the mass production of active substances related to acne treatment is difficult.

In particular, natural products are composed of various components, and the method of separating and purifying effective active substances depends on the physicochemical properties of the active substances, and among the methods of separation and purification based on several different principles, it is possible to effectively combine appropriate methods. There is a lot of difficulty in extracting and separating substances having anti acne activity from natural products.

Meanwhile, the crowd (Dry, Dryopteris) crassirhizoma ) is a perennial herb of ferns, and is a fern plant in Korea, Japan, Sakhalin, Kuril Islands, and northeastern China. The main components of the audience are 17-α (H) -trisnorhopanone, 17-β (H) -trisnorhopanone, campesterol, diplopterol, dryocrassin, dryocrassin ABBA, dryocrassin ABBP, dryocrassol, dryocrassyl acetate, fernene, fern-9 (11)- en-12-one, flavaspidic acid, hop-22 (29) -ene, and isoadiantone. Young leaves are used for edible, and oriental medicine uses root stem as a medicine. The spectators exhibit antiparasitic activity, antipyretic effect, detoxification effect, cold virus suppression effect, antibacterial and anticancer activity, and the water decoction is known to exhibit inhibitory action against skin fungi. It has also been reported that the spectators exhibit uterine excitability, hemostatic action, analgesic action and anti-inflammatory action.

In Japanese Patent Laid-Open No. 5-331041, a whitening cosmetic prepared by extracting an ingredient effective for whitening from natural products such as pomegranate bark and the like is disclosed. In this case, 300 g of methanol or 50% methanol aqueous solution was added to 10 g of the dried tube, the mixture was left to stir for 5 days, filtered, and then the cosmetic composition was prepared from the spectral extract obtained by freeze-drying to inhibit the activity of tyrosinase. By measuring, the use of the tyrosinase inhibitory effect of the spectral extract for whitening cosmetics is disclosed.

However, the fact that the spectators show strong activity against acne bacteria or that it is effective for the prevention and treatment of acne has not been reported so far, and extracts the ingredients that are effective for the prevention and treatment of acne from the audience and use them as cosmetics for acne skin. No technique has been disclosed.

Accordingly, an object of the present invention is to minimize the side effects associated with existing acne treatment methods, and to provide a new use of the crowd for preventing and treating acne in a way that can be continuously used and has excellent safety.

In particular, it is an object of the present invention to provide a use of the spectral extract to inhibit the growth of Propionibacterium acnes .

It is also an object of the present invention to provide a cosmetic composition for acne skin containing the spectral extract.

The spectral extract of the present invention is contained in the spectator itself, leaching, elution from the spectral part, or re-condensation of some or all of the leachate, or acupuncture, premise, regular, flow extract, etc. prepared by drying the concentrate again. It is a concept that includes the chemical itself, which is used and has a major effect.

The preferred spectral extract of the present invention is a high-liquid extract of a component that inhibits the growth of propionibacterium acnes or a component that exhibits an acne prevention and treatment effect by adding a predetermined extraction solvent to the spectator. It can be the primary extract in the tubes obtained by solid-liquid extraction.

The spectral of the present invention is prepared by completely drying and then pulverizing, the spectral extract of the present invention is added to the hydrophilic organic solvent, such as methanol, ethanol, or a mixed solution of a hydrophilic organic solvent and water as an extraction solvent while being deposited for a predetermined time After stirring at appropriate time intervals, the obtained solution can be obtained according to the usual natural product extraction method by filtration.

Preferred extraction solvents include one or more pure solvents selected from the group consisting of anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol, and water. Combined mixed solvents may be used, and the amount of extractant added to the plant material may be about 1 to 50 times the total volume of the plant material.

More preferably, as the extraction solvent of the primary extract of the present invention, one pure solvent selected from anhydrous methanol, hydrous methanol, anhydrous ethanol and hydrous ethanol, or a mixed solvent of one of the methanol and one of the ethanol It is preferable to use.

At this time, the amount of the extraction solvent is preferably 5 to 20ml per 1g dry weight in the tube. Primary extraction may be performed by a conventional general solvent extraction method, such as extraction by immersion at room temperature for 1 to 7 days or extraction for about 1 to 5 hours at a heating condition of 50 ~ 95 ℃.

According to the present invention, it is possible to maximize the efficacy of the active ingredient while minimizing the loss of the active ingredient associated with acne prevention and treatment from the crowd.

In addition, the spectral extract of the present invention has the effect of preventing and treating acne by the liquid-liquid extraction method by adding a new solvent to the spectral primary extract obtained by the solid-liquid extraction. It may be a secondary extract obtained by extracting the active ingredient.

Liquid-liquid extraction usually uses distribution equilibrium with various solvents in order to increase the activity of the active substance or to separate and purify only its components. As a secondary extraction solvent, ethyl acetate, hexane, chloroform, butanol, etc. Can be used. In the present invention, by using hexane as a secondary extraction solvent for liquid-liquid extraction, the separation or activity of the active ingredient having an acne prevention and treatment effect is increased.

That is, in order to further increase the activity of the active ingredient having an acne prevention and treatment effect from the primary extract of the present invention, the spectral extract of the present invention completely dissolves the primary extract of the tube obtained by the solid-liquid extraction in distilled water. After making an aqueous solution, the hexane (hexane) is added to the aqueous solution and a distribution process for eluting the active ingredient material in the hexane layer, and then recovering the hexane layer eluting the active ingredient material, to remove the hexane from It can be a secondary extract of spectators containing ingredients that have an effect on preventing and treating acne.

The cosmetic composition for acne skin of the present invention is characterized in that it comprises an active ingredient which has a preventive and therapeutic effect of acne derived from the spectral extract, the content of the tract extract is preferably 0.001 to 20% by weight, 0.5 ~ More preferably, it is 10 weight%. This may cause side effects such as skin irritation and toxicity at high concentrations of more than 20% by weight, because the effect is weak at low concentrations of less than 0.001% by weight.

The cosmetic composition of the present invention, in addition to the above-mentioned extract in the spectators, if necessary, various components generally used in cosmetics, such as water-soluble components, powder components, oil, surfactants, It is possible to mix a moisturizer, a viscosity modifier, a preservative, an antioxidant, a fragrance, and a pigment.

Cosmetic compositions containing the extracts of the present invention of the present invention may be prepared in any formulation commonly prepared in the cosmetic industry, for example, solutions, suspensions, pastes, gels, creams, lotions, powders, soaps, oils, Powder foundations, emulsion foundations, wax foundations and sprays and the like can be formulated, but is not limited thereto. More specifically, it is prepared in the form of a flexible lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, latex foundation, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, soap or powder Can be.

The spectral extract of the present invention can be used as an external preparation for acne skin in addition to the cosmetic composition for acne skin.

Hereinafter, the present invention will be described in detail with reference to more specific examples, but the present invention is not limited only to the following examples.

[ Example  1] Preparation of Spectators and Preparation of Spectrum Primary Extracts

The crowd purchased dry outposts sold in the market and prepared the powder using a grinder.

10 L of methanol was added to 1 kg of the prepared tube, followed by extraction with stirring at 300 rpm for 3 hours at room temperature. Then, the extraction solution was collected by filtration using filter paper, and the recovered solution was concentrated under reduced pressure at 47 ° C. using a distillation apparatus equipped with a cooling capacitor to prepare a primary extract in the tube.

Then, ethanol was added instead of methanol to prepare a secondary extract in the same manner by the same method.

[ Example  2] Preparation of Spectral Secondary Extracts

The primary extract of the tube in the state of completely removing the solvent was completely dissolved in distilled water to make an aqueous solution. The solution was then placed in a separatory funnel and the same volume of hexane (n-Hexane) was added. At this time, the separation funnel was shaken sufficiently so that the two solvents were well mixed so that the material could be sufficiently extracted in the hexane layer. After sufficiently shaking the separatory funnel fixed to the holder and allowed to stand until the two layers were separated, each of them was recovered, and the distilled water layer was placed in the separatory funnel again to repeat the above process to recover only the hexane layer. The hexane layer prepared a secondary extract in the tube by completely removing the solvent using a rotary vacuum concentrator.

[ Test Example ]

One. Use strain , Medium and culture conditions

The strain used in the present invention is Propionibacterium which is the cause of acne acnes KCTC3314, these strains were used by the Korea Gene Bank.

Reinforced clostridial (RC) medium (Merck, Germany) was used as a culture medium for acne bacteria, and P. acnes was activated 3 days before the experiment while stored at 4 ° C. The bacteria were inoculated in the culture medium and sealed in an anaerobic jar using a Gaspack system (Merck AnaerocultGaspack system, Germany), followed by anaerobic culture for 3 days in an incubator at 37 ℃.

2. Determination of antimicrobial activity against acne

Antimicrobial activity was measured using the paper disk diffusion method and the minimum inhibitory concentration (MIC).

(1) paper disk diffusion method

The strain was incubated in a Reinforced clostridial (RC) liquid medium for 3 days, and then the bacterial suspension was adjusted to a constant concentration (absorption value at 0.5 at an optical density of 620 nm) to agar plate using a sterile swab. She said. After drying the plate, paper disk (Φ 8mm, Adventec. USA) was placed on the plate surface inoculated with the strain, and the primary or secondary extract of the tube was diluted in DMSO and absorbed by 250 ~ 500㎍ / 50μl / disk. I was. The plate treated with the sample was incubated in an anaerobic jar using a Gaspack system for 3 days, and then the antimicrobial activity was measured by the size of the inhibition zone around the disc.

At this time, triclosan (triclosan), which is widely used as an antimicrobial agent of the cosmetic composition, was used.

<Table 1> Size of acne bacteria growth inhibitory ring of primary extract

Sample Name Treatment concentration (㎍ / 50µl / disk) Restraining ring (mm) One Triclosan 500 35 2 Primary extract of Example 1-Extraction solvent: methanol 500 28 3 Primary extract of Example 1-Extraction solvent: Ethanol 500 34 4 Secondary extract of Example 2-primary extraction solvent: ethanol, secondary extraction solvent: hexane 250 40

From the results of Table 1, it can be seen that there is a strong anti-acne bacterium activity in the primary extract (extract solvent: ethanol) and the secondary extract in the tube. In particular, the anti acne activity of the primary extract in the ethanol extract was found to be similar to the activity of triclosan used as a control. Even when methanol was used as the extraction solvent, the size of the growth inhibitory ring was found to be 28 mm, indicating that propionibacterium acne had excellent growth inhibition ability. In addition, it can be seen that the secondary extract of the spectral has a strong anti-acne bacterium activity even at a much lower level than the concentration of the primary extract of the spectral.

(2) Minimum inhibitory concentration (MIC)

MIC evaluation was performed with some modifications of the broth-dilution method using 96-well plates (K. Yasunaka et al ., Antibacterial activity of crude extracts from mexican medicinal plants and purified coumarins and xanthones, J. Ethnopharmacol. , 97 , 293 (2005)). The acne bacteria cultured for 72 hours were adjusted to the value of McFarland standard 0.5, and 20 µl of each strain containing 160 µl of the medium diluted to 1.5x10 ⁸ cells / ml was inoculated. Primary or secondary extracts in the tube were dissolved in DMSO, and 20 µl each was added to the 96-well plate containing the strain and the medium using RC liquid medium and mixed well. 10% DMSO was used as a control. After incubating 96-well plates inoculated with bacterial dilutions and samples using an anaerocult gaspack system for 3 days, the minimum concentration at which bacterial growth was not determined was determined by MIC. Triclosan was used as a control.

TABLE 2

Sample Name Minimum inhibitory concentration One Triclosan 0.004mg / ml 2 Primary extract of Example 1 (extraction solvent: ethanol) 0.0016mg / ml

As can be seen from the above results, the primary extract of the spectral extract has a much lower minimum inhibitory concentration than the control triclosan, and the spectral extract of the present invention and the cosmetic composition containing the same prevent and treat acne and grow propionibacterium acne. It can be seen that the inhibitory effect is very excellent.

Cosmetic composition comprising an active ingredient for preventing and treating acne derived from the spectral extract of the present invention may be the content of the spectral extract to 0.001 to 20% by weight. At this time, the content of the spectral extract is more preferably 0.5 to 10% by weight.

Cosmetic composition containing the spectral extract of the present invention can be used in a variety of cosmetics and cleansing agents for acne skin, the dosage form can be arbitrarily selected. Products to which the present composition may be added include cosmetics such as creams, milk lotions, foundations, skins, and the like, which are applied to various skins, and cleansing, face washes, soaps, treatments, and essences.

The following shows an example of the specific formulation of the cosmetic composition containing the spectral extract of the present invention, the formulation comprising the cosmetic composition of the present invention is not limited thereto.

Formulation Example 1 Skin Toner

Ingredient Name Content (% by weight) Spectral secondary extract 2.5 glycerin 5.0 1,3-butylene glycol 3.0 ethanol 5.0 Polyoxyethylene nonylphenyl ether 0.5 Spices 0.2 antiseptic A reasonable amount Purified water to 100

<Formulation Example 2> Converging Lotion (astringent)

Ingredient Name Content (% by weight) Spectral secondary extract 5.0 glycerin 3.0 Citric acid 0.1 ethanol 10.0 Polyoxyethylene oleyl ether 1.0 Spices 0.1 Sorbitol 2.0 antiseptic A reasonable amount Purified water to 100

Formulation Example 3 Lotion (Emulsion)

Ingredient Name Content (% by weight) Spectral secondary extract 4.0 glycerin 5.0 1,3-butylene glycol 8.0 Squalane 10.0 Monooleic acid polyoxyethylene sorbitan (6E.0) 2.0 Triethanolamine 1.5 Glyceryl Stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxy Vinyl Polymer 0.1 Arginine 0.1 Spices 0.2 antiseptic A reasonable amount Purified water to 100

<Formulation Example 4> Cream

Ingredient Name Content (% by weight) Spectral secondary extract 1.0 glycerin 5.0 Stearic acid 8.0 Squalane 5.0 Self-emulsifying glycerin monostearate 2.5 Monostearic acid polyoxyethylene sorbitan 1.5 Propylene glycol 4.0 Stearyl glycyrrhetinate 0.2 vaseline 2.0 Antioxidant A reasonable amount Spices 0.2 antiseptic A reasonable amount Purified water to 100

Formulation Example 5 Latex Liquid Foundation

Ingredient Name Content (% by weight) Spectral secondary extract 0.5 Cetostearyl alcohol 0.5 Liquid lanolin 2.0 Liquid paraffin 3.0 Myristic acid isopropyl 5.0 Carboxymethyl Cellulose Sodium 0.2 Bentonite 0.5 glycerin 3.0 Titanium dioxide 8.0 Talc 4.0 Pigmentation Pigment A reasonable amount Triethanolamine 1.0 Stearic acid 2.0 Antioxidant A reasonable amount Spices 0.2 antiseptic A reasonable amount Purified water to 100

<Formulation Example 6> Soap

Ingredient Name Content (% by weight) Spectral secondary extract 1.0 Palm oil fatty acid 1.0 Titanium dioxide 0.3 Sodium Ethyleneamine Tetraacetate 0.1 Spices 0.5 Pigment A reasonable amount glycerin 1.0 Antioxidant A reasonable amount Soap Chips (Base) to 100

According to the present invention, it is possible to see the effect of acne prevention and treatment by using the spectators, and extractable separation that can maximize the activity of the active ingredient exhibiting acne prevention and treatment effect is possible, comprising the spectral extract according to the present invention The cosmetic composition for acne skin can effectively prevent and treat acne by effectively inhibiting the growth and proliferation of propionibacterium acne. And, because the crowd is a safe plant that has been edible for a long time, according to the present invention has no side effects compared to the conventional acne treatment method, it is possible to obtain a cosmetic composition for acne skin with excellent safety and excellent use.

Claims (7)

A spectator for preventing and treating acne extracted by adding any one selected from water, a hydrophilic organic solvent, a mixed solvent of water and a hydrophilic organic solvent, and a mixed solvent of two or more hydrophilic organic solvents to a dryopteris crassirhizoma extract. The method of claim 1, The spectral extract is spectral extract, characterized in that to inhibit the growth of Propionibacterium acne ( Propionibacterium acnes ). The method of claim 2, The hydrophilic organic solvent is a spectral extract, characterized in that methanol or ethanol. The method of claim 3, wherein The spectral extract, characterized in that the second extract the active ingredient showing the acne prevention and treatment effect by using hexane (hexane) as a secondary extraction solvent in the spectral extract. Cosmetic composition for acne skin containing the spectral extract of any one of claims 1 to 4. The method of claim 5, The content of the extract in the tube is a cosmetic composition for acne skin, characterized in that 0.001 ~ 20.0% by weight. The method of claim 6, The formulation of the cosmetic composition for acne skin is a cosmetic composition for acne skin, characterized in that any one selected from flexible lotion, astringent lotion, lotion, cream, emulsion foundation, soap.