KR20080001031A - A cosmetic composition for wrinkle improvement containing a extract of prunella vulgaris - Google Patents

Abstract

A cosmetic composition comprising the extract of Prunella vulgaris is provided to maintain and increase elasticity of the skin by promoting biosynthesis of elastin and inhibiting synthesis of elastase, and improve inhibition of oxidation, so that it is useful for improving skin wrinkles. A cosmetic composition for improving skin wrinkles comprises 0.005-50 wt.% of the extract of Prunella vulgaris which is prepared by extracting Prunella vulgaris with a polar solvent selected from water, C1-C4 lower alcohol and a mixed solvent thereof, and has a formulation selected from skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.

Description

A cosmetic composition for wrinkle improvement containing a extract of Prunella vulgaris}

The present invention relates to a cosmetic composition for improving wrinkles containing hachochos extract. Specifically, the present invention relates to a cosmetic composition for improving wrinkles, which contains a hachocho extract and has an excellent effect of promoting elastin biosynthesis and inhibiting elastay synthesis, and also relates to a cosmetic composition having an excellent antioxidant effect.

Cosmetics are deeply involved in our daily lives and used by many people. Before the development of human civilization, the purpose of cremation was to protect the body from nature.However, with the development of science and civilization, the purpose of cremation is to keep the body clean and beautiful, and to delay the aging to make a beautiful and pleasant life. Changed. The function of cosmetics is not only clean and aesthetic, but also free radicals and free radicals caused by external factors such as moisture, pollutants and stress, ultraviolet rays, and internal factors such as deterioration of immune cells and deterioration of cell activity. This is to remove or suppress the cause of skin aging such as protein, nucleic acid, and cell membrane lipid destruction by peroxides. In addition, cosmetics having a whitening, skin wrinkle improvement and suppression, and sunscreen functions to meet the consumer's desire for high-performance cosmetics continue to be developed. In particular, wrinkle improvement and suppression products are of great interest to consumers seeking to stay younger.

Wrinkles are formed by repetitive movements of muscles in a specific direction for a long time, affected by age, external environment, and UV irradiation. Damage to skin cells caused by these factors causes various aging phenomena. The collagen, elastin, and GAGs constituting the dermal matrix are not easily produced and degraded, resulting in a loss of firmness and wrinkles.

Currently, wrinkle improvement cosmetics are known as retinoids, adenosine, animal placental protein, chlorella extract and the like. The most well known retinol is a substance that promotes collagen synthesis, but it is unstable and has limited use due to safety problems such as irritation and seizures when applied to the skin. Chlorella extract, etc., is insignificant and it is difficult to expect an improvement in skin wrinkles. Known.

      On the other hand, the skin is exposed to ultraviolet light, and photochemical reactions that produce reactive oxygen species continue to occur, and these reactive oxygen species lead to damage to skin cells and tissues. They break the antioxidant defenses, which consist of antioxidant and non-enzymatic antioxidants, shifting the oxidant / antioxidant balance towards the oxidative state. Therefore, for the protection of a healthy body, a system that can effectively protect the generated free radicals must be included in the prescription of cosmetics. In other words, it is necessary to build an antioxidant defense system for skin that maintains an oxidant / antioxidant balance.

Materials known to have antioxidant activity so far include ascorbic acid, DL-alpha-tocopherol, beta-carotene, dibutylhydroxytoluene (BHT), and the like. However, since these substances are used as synthetic products, they may cause skin safety problems in long-term use or in large amounts, and thus, there is an urgent need for the development of new antioxidant cosmetics that can be applied to the skin more safely and usefully.

On the other hand, hachocho extract is a cosmetic composition for removing skin impurities such as blemishes, freckles (Korean Patent Publication No. 10-2002-0093655), cosmetics for treating atopic dermatitis (Korean Patent Publication No. 10-2003-0032631), whitening Cosmetic cosmetics (Korean Patent Publication No. 10-2005-0018321) and the like are known.

An object of the present invention is to improve the wrinkles of the skin by containing the hachori extract. In particular, it has the effect of increasing the synthesis of elastin and inhibiting the activity of elastase, and to maintain the elasticity and increase the skin's elasticity. In addition, it aims to maintain healthy skin by enhancing the antioxidant effect.

As a result of studying various herbal extracts in order to achieve the technical problem, it is an object of the present invention to improve the skin condition by providing it to the cosmetic composition because the hachocho extract has excellent wrinkle improvement and antioxidant effect.

The present invention provides a cosmetic composition for anti-wrinkle containing the hachocho extract. In particular, it promotes the production of elastin and inhibits the synthesis of elastin, characterized in that it is utilized for skin wrinkle improvement.

In addition, the composition is characterized in that it is also used as an antioxidant cosmetic.

Hagocho is also called nectar and is a perennial herb with nectar. It is native to Korea and Manchuria. It is a perennial herb and grows in mountain fields and roadside grass fields with a height of 20-30cm. The main stem is square, with white hairs all over, and the flowers are purple and close to the flowers. The leaves face each other and have a serrated tooth shape. Use dried herbs or flowers of nectar as a Chinese medicine. Ingredients include triterpenoids, oleanolic acid, ursolic acid, rutin, hyperoside, and caffeic acid. In civilian use, it is used for tonic, hypertension, uterus, menstrual irregularity, diuresis, eye disease, goiter, gonorrhea, nausea, head, fever, playing window, fever and fever. It is used for various treatments such as adenocarcinoma and lymph node tumors. It also has the effect of inhibiting the growth of pathogenic microorganisms such as tuberculosis bacilli, dysentery bacilli, staphylococci, and hemolytic streptococci. According to <Agreement Relief>, it is effective for urinary stones and removes the heat of the liver and phlegm and improves eye diseases, headaches and dizziness. In <Dictionary Synonym>, there are detoxification, coercive action, diuretic action, bacteriophage, etc. It is said that it can be used for playing window, permanent, yeastitis, head window, carcinoma, hepatic, edema, lobster, hypertension, pulmonary tuberculosis, epidemic lobe. The Chinese medicinal dictionaries have been shown to be effective in Nag, permanent, acute mastitis, breast cancer, ocular pain, and dizziness. Jeonnam Herbal Herbs is said to be effective in the treatment of wounds cut in guan wasa, bruises and knives. Korea Herbal Herbs Book is said to be effective in tuberculous lymphadenitis, boils, and hypertension.

Hachocho extract according to the present invention can be prepared as follows.

Wash the vinegar with water and dry it in the shade. Dry vinegar is placed in an extraction container and extracted for a certain period of time at an appropriate temperature in an appropriate amount of extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. Preferably, hot water is extracted by adding ethanol.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of water bath extraction.

The deposition temperature is 30 ~ 70 ℃, more preferably 40 ~ 60 ℃, most preferably maintained at about 50 ℃ is most effective in the prevention of decay, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After obtaining the Hachocho extract, the solids are removed using a filter paper, the suspension is centrifuged, and the supernatant can be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

Hachocho extract according to the present invention is excellent in anti-wrinkle effect, promotes the production of elastin, inhibits the production of elastin and shows the use of skin condition improvement. The cosmetic composition of the present invention improves wrinkles of the skin by a cell proliferation promoting effect and is excellent in stability in the formulation. It also has an excellent antioxidant effect. This fact is specified in the following examples.

In the composition of the present invention, the edible extract may be contained in an amount of 0.005 to 50 wt% based on the total weight of the composition.

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the Hachowort extract.

Cosmetics prepared with a cosmetic composition containing the edible extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to the cosmetic containing the extract of the haejucho of the present invention, by containing a dermatologically acceptable medium or base can be prepared in the form of adjuvants that can be applied topically or systemically applied commonly used in the field of dermatology.

Suitable formulations of cosmetics include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics, obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. Can be provided in the form of a vesicle dispersant, cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the Hagocho extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Hacho Extract

50 g of vinegar was finely crushed in a crusher, immersed in 200 ml of 100% ethanol, and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Experimental Example 1 Cell Proliferation Effect

In order to see the skin cell proliferation effect of Prunella vulgaris extract, human normal fibroblasts were inoculated to 1 × 10 ⁴ cells in each well of a 96-well microplate. Incubate for 24 hours in Dulbecco's Modified Eagle's Medium. After the culture, the extract of Preparation Example 1 prepared above was replaced with DM medium without serum adjusted to a final concentration of 50 to 500 µg / ml, followed by further incubation for 24 hours. MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium, 3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium after incubation : 5 mg / ml) 10 µl of solution was added thereto, and the reaction solution was removed after standing for 4 hours. 100 μl of dimethyl sulfoxide solution was added to each well to dissolve the stained cells, and the absorbance at 570 nm was measured with a microplate reader.

By performing the above procedure, the cell proliferation effect was obtained from Equation 1 below, and the results are shown in Table 1 below.

(Equation 1)

Cell proliferation effect (%) = {(absorbance at extract treatment-absorbance of control) / absorbance of control} X 100

Sample (µg / mL) Cell proliferation effect (%) Control - 50 38.2 100 51.9 200 70.5 300 75.8 500 83.7

      According to the above Table 1, Hachocho extract can be confirmed that the cell proliferation effect is excellent in improving the skin wrinkles.

Experimental Example 2 Elastin Synthesis Effect

Human normal fibroblasts isolated from 10 ⁵ newborn skins were cultured in 6-well plates for 24 hours, and then the extract samples of Preparation Example 1 were treated with 50-500 µg / ml. After 24 to 48 hours of incubation, the amount of elastin newly released by the fibroblasts was measured using a commercially available Fastin ™ elastin assay kit. The amount of elastin was corrected by cell number or protein amount. The results are shown in Table 2.

Sample (µg / mL) Elastin Synthesis Increase (%) Control - 50 18.9 100 34.2 200 65.2 300 79.8 500 85.4

     According to the above Table 2, Hachocho extract shows an excellent effect on the increase in elastin synthesis, it can be confirmed that there is a skin wrinkle improvement effect through this.

(Experimental example 3) Late stay activation inhibitory effect

When elastins, an enzyme that degrades elastin, is active, elastin, an elastic fiber, is broken down to accelerate skin wrinkle formation. Therefore, in this experimental example, the inhibition rate of the elastase of the extract of Preparation Example 1 was measured. Degradation of N-succinyl- (alanine) -3-p-nitroanilide, which is the substrate of elastase, results in p-nitroaniline, which is yellow in color. The degree of color development was measured by absorbance using a spectrophotometer to evaluate the rate of inhibition of elastase activity (The synthesis and analytical use of a high sensitive and convenient substrate of elastase, Biochemical Medicine 11, 350-357, 1974). The method of calculating the activity inhibition rate of the elastase is shown in Equation 2 below, and the detailed method is as described in the above document. The results are shown in Table 3.

(Equation 2)

     % Elastase activity inhibition = (1-B / A) × 100

     A: OD 400 without inhibitor

     B: OD 400 in the presence of inhibitor

Sample (µg / mL) Elastase Synthetic Inhibitory Effect (%) Control - 50 10.3 100 22.6 200 40.2 300 62.5 500 70.5

      According to the Table 3, the Hachocho extract is excellent in inhibiting the elastase synthesis can be confirmed that there is an effect to improve the skin wrinkles.

Experimental Example 4 Antioxidant Effect

In order to confirm the antioxidant efficacy, a free radical 1,1-diphenyl-2-picrylhydrazyl (1,1-Diphenyl-2-picrylhydrazyl; hereinafter referred to as DPPH) was used. DPPH was used by dissolving in ethanol to 2 × 10-4 M concentration. 0.15 mL of DPPH, 0.15 mL of an extract sample of Preparation Example 1 (1000 ppm-30 ppm), or vitamin C (1000 ppm-30 ppm) was added thereto, followed by reaction at room temperature for 30 minutes, followed by measurement of absorbance at 570 nm. Modified) DPPH scavenging effect was compared to IC ₅₀ (50% Inhibitory activity) for the sample with a DPPH radical scavenging activity value of 50% or more calculated using Equation 3 below.

(Equation 3)

      DPPH radical scavenging activity (%) = ((A control-B control)-(A sample-B sample)) / (A control -B control) × 100

    A control: Absorbance of control group without sample

    A sample: Absorbance of reaction group to which sample is added

    B control: Absorbance of control group without DPPH

    B sample: Absorbance of reaction group without DPPH

     As a result, Table 4 shows the results of measuring the scavenging effect of free radicals using DPPH at 1000 ppm concentration of haecho extract and vitamin C (Roche, Switzerland) for each concentration.

Sample (µg / mL) IC ₅₀ (μg / ml) Vitamin C 3.86 50 32.41 100 15.19 200 8.25 300 6.72 500 5.48

According to Table 4, Hachocho extract has an excellent antioxidant effect through which it can be confirmed that effective in improving the skin condition.

Preparation Example 2 Examples and Comparative Examples

To prepare a cosmetic composition containing the extract of Hagocho of Preparation Example 1, the cosmetic composition lacking the haegocho extract was prepared in the composition ratio of Table 5 to compare with this.

division Content (% by weight) ingredient Example Comparative example Hagocho extract 1.0 - Butylene glycol 2.0 2.0 Propylene glycol 6.0 6.0 Carboxy Vinyl Polymer 0.5 0.5 Beeswax 2.0 2.0 vaseline 7.0 7.0 Polysorbate 60 2.0 2.0 Sorbitan sesquioleate 2.5 2.5 Squalane 3.0 3.0 Liquid paraffin 10.0 10.0 Triethanolamine 0.5 0.5 Tocopheryl Acetate 0.1 0.1 Spices, preservatives a very small amount a very small amount Purified water Remaining amount Remaining amount Sum 100 100

Experimental Example 5 Clinical Effect of External Wrinkle Improvement

The clinical effect of skin wrinkle improvement on cosmetics containing Hachocho extract of the present invention was measured. Divided 20 women (age 44.5 years old) with 2 groups (A and B) and applied Examples to Group A and Comparative Examples to Group B for 12 weeks around wrinkled eyes. 2 times / day, 0.2 g / time), and the degree of improvement of skin wrinkles (ΔW) was measured by classifying the objective evaluation of the skilled person and the subjective evaluation of the subject into the following seven levels. The results are shown in Tables 6 and 7 below.

In addition, the skin wrinkle effect (Skin Visiometer SV 600 FireWire, CK Electronic GmbH) and the elasticity measuring instrument (Cutometer MPA 580, CK Electronic GmbH) were used to measure wrinkle improvement and elasticity of the skin. Is shown in Table 9.

* Skin wrinkle evaluation criteria

Deteriorate very badly -3 worse -2 Slightly worse -One No change 0 Slightly improved One Improving 2 Very improved 3

Objective evaluation of the skilled person Average wrinkle improvement (△ W) Example Comparative example T0 T12 T0 T12 Subject 1 0 2 0 One Subject 2 0 3 0 0 Subject 3 0 One 0 0 Subject 4 0 2 0 0 Subject 5 0 3 0 0 Subject 6 0 2 0 One Subject 7 0 One 0 0 Subject 8 0 2 0 0 Subject 9 0 3 0 0 Subject 10 0 3 0 One △ W (T12-T0) 2.2 0.3

Subjective Evaluation of the Subject division Average wrinkle improvement (△ W) Example Comparative example T0 T12 T0 T12 Subject 1 0 One 0 One Subject 2 0 3 0 0 Subject 3 0 2 0 0 Subject 4 0 3 0 0 Subject 5 0 One 0 One Subject 6 0 3 0 0 Subject 7 0 2 0 0 Subject 8 0 3 0 One Subject 9 0 2 0 0 Subject 10 0 2 0 One △ W (T12-T0) 2.2 0.4

As a result of the test, after 12 weeks, the examples containing the vinegar extract showed that the average wrinkle improvement had a significant wrinkle improvement effect compared to the comparative example, with the objective evaluation of the skilled person and the subjective evaluation of the subject being 2.2, respectively.

division Wrinkle improvement effect Elasticity Average depth of wrinkles (%) Roughness average (%) Skin adsorption degree (%) Example T0 100.0 100.0 100.0 T12 75.5 62.4 66.5 Comparative example T0 100.0 100.0 100.0 T12 99.1 96.4 97.5

In Table 8, the average depth of the wrinkles is divided into five equal parts of the sample made of silicone rubber at the site where wrinkles are to be measured, and represents the average value of the difference between the highest and lowest points of the wrinkle height in each of five places. The roughness mean represents the mean value of the difference in wrinkle depth at each measurement site (dividing the whole into 255 measurement site points) from the average value of the total wrinkle depth in the sample. The lower the average depth and roughness of the wrinkles, the smaller the wrinkles are, and the smaller the skin aging is.

      From the results shown in Table 8 above, when the relative numerical calculation before / after the application of the example, the average depth value of the wrinkles were improved by 22.1% after the application of the example when viewed as 100%, wrinkle improvement improved by roughness average As it can be seen that the effect is improved by 37.4%, it can be seen that the cosmetic composition containing the vinegar extract according to the present invention has a considerable effect on the improvement of wrinkles of the skin.

       In addition, in Table 8, the degree of skin adsorption indicates the degree of adsorption of the skin into the cutometer measuring device (penetration depth of the skin into the aperture), and the smaller the value is, the more preferable. When viewed as 100% before application, it can be confirmed that the elasticity of the skin is improved to a significant extent by 32.23% after the application of the above example.

      As a result, the fact that it can be confirmed through the results shown in Table 8 is that the cosmetics containing the extract of Hagocho according to the present invention is to enhance the skin wrinkle improvement effect and elasticity effect, showing an excellent effect of preventing skin aging Able to know.

      On the basis of the results of the previous experimental example, to present a cosmetic composition containing the hachocho extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Flexible Lotion

     Formulation example of the flexible lotion containing a vinegar extract was prepared as shown in Table 9.

Raw material Content (% by weight) Hagocho extract 1.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

Formulation Example 2 Nutrition Cream

Formulation example of nutrition cream containing the extract of Hagocho was prepared as shown in Table 10.

Raw material Content (% by weight) Hagocho extract 2.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Remaining amount

As described above, the cosmetic composition containing the hachocho extract is effective in improving skin wrinkles, in particular, it is excellent in promoting elastin biosynthesis and inhibiting elastin synthesis, and also excellent in antioxidant effect. The cosmetic composition containing the Hachocho extract is useful for preventing skin aging cosmetic composition through the wrinkle improvement effect.

Claims (8)

Wrinkle improvement cosmetic composition containing a vinegar extract. The cosmetic composition for improving wrinkles according to claim 1, wherein the composition promotes elastin synthesis and inhibits elastase activity. Antioxidant cosmetic composition containing a haze extract. The cosmetic composition according to any one of claims 1 to 3, wherein the extract is Prunella vulgaris. The cosmetic composition according to any one of claims 1 to 3, wherein the extract is hydrothermally extracted. The cosmetic composition according to claim 5, wherein the hot water extraction is performed by adding a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The cosmetic composition according to any one of claims 1 to 3, wherein the content of the extract is 0.005 to 50% by weight based on the total weight of the cosmetic composition. The composition according to any one of claims 1 to 3, wherein the composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream , Essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow Cosmetic composition to be.