KR20100000112A - A cosmetic composition for preventing and improving dermatitis - Google Patents

Abstract

PURPOSE: A cosmetic composition containing natural products extract for preventing and relieving dermatitis is provided to suppress nitrogen oxide(NO) generation and lipid peroxidation and prevent dermatitis. CONSTITUTION: A cosmetic composition for preventing and relieving dermatitis contains natural product extract of Taraxacum platycarpum, Phaseolus radiates, Betula phatyphylla japonica and Rumex crispus in a weight ratio of 1-5:1-2: 1-2:1-2. The content of natural product extract is 0.005~50 weight% based on total cosmetic weight. The cosmetic composition is used in the form of skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage lotion, moisture cream, hand cream, nutrition essence or eye shadow.

Description

Cosmetic composition for preventing and improving dermatitis

The present invention relates to a cosmetic composition for preventing and improving dermatitis, in particular, dandelion ( Taraxacum) platycarpum ), mung bean ( Phaseolus) Radiatus ), birch ( Betula phatyphylla japonica ) and Rumex crispus extracts contain a natural extract mixture as an active ingredient, antioxidant effect, NO production inhibitory effect, lipid peroxidation inhibitory effect, anti-inflammatory effect, cell It relates to a cosmetic composition for preventing and improving dermatitis due to the increase of the proliferative effect.

Antioxidation refers to the inhibition of various oxidation reactions in the body. Oxidation refers to a phenomenon in which various unsaturated fatty acids present in the body are oxidized to form lipid peroxides and aging is accelerated by the increase of these substances. In order to prevent the aging caused by the free radicals formed by the oxidation reaction, SOD (superoxide dismutase), CAT (Catalase), GSHpx (Glutathione peroxidase), etc. exist to protect the body from oxidative stress. .

Inflammatory reactions are known to have a number of causes, one of which is the cytokine produced by macrophages and NO (nitric oxide). NO is produced by iNOS in various pathological processes such as inflammation and cancer. INOS is produced from L-arginine as an important enzyme that regulates inflammation, vasodilation, neurotransmission, tumor cell and homeostasis.

Skin cells are damaged by such oxidative stress and inflammatory reactions. If damaged skin cell proliferation is not properly performed, the skin is further damaged, so it is also important to actively proliferate the skin cells.

The purpose of this study was to develop natural products with excellent anti-dermatitis prevention and improvement effect due to antioxidant effect, NO production inhibitory effect, lipid peroxidation inhibitory effect, anti-inflammatory effect, and cell proliferation effect.

The present invention was intended to develop a substance that exhibits an antioxidant effect, an NO production inhibitory effect, a lipid peroxidation inhibitory effect, an anti-inflammatory effect, and a cell proliferation effect.

Accordingly, the present invention is a natural extract mixture formed by mixing the extract of dandelion, mung bean, birch and thorax extracts by the synergism between the extracts antioxidant effect, NO production inhibitory effect, lipid peroxidation inhibitory effect, anti-inflammatory effect, cell proliferation effect The present invention has been completed by finding out that it is effective in preventing and improving dermatitis.

Dermatitis prevention and improvement cosmetic composition for achieving the above object of the present invention comprises the following configuration.

According to an embodiment of the present invention, the cosmetic composition for preventing and improving dermatitis according to the present invention is formed by mixing extracts of dandelion, mung bean, birch and russetula.

According to another embodiment of the present invention, the natural extract mixture, dandelion: mung bean: birch: Sorbus extracts are mixed in a weight ratio of 1 to 5: 1 to 2: 1 to 2: 1 to 2 as an active ingredient, respectively. It features.

According to another embodiment of the present invention, the composition is characterized by having an antioxidant effect, NO production inhibitory effect and lipid peroxidation inhibitory effect.

According to another embodiment of the present invention, the composition is characterized by having an anti-inflammatory effect and a cell proliferation effect.

According to another embodiment of the present invention, the natural extract mixture is characterized in that 0.005 to 50% by weight relative to the total weight of the cosmetic composition.

According to another embodiment of the present invention the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition It is characterized in that it is formed of one of the formulations selected from the group consisting of essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.

The present invention is a natural extract mixture formed by mixing the extracts of dandelion, mung bean, birch and russetula extracts by the synergism between the extracts, antioxidant effect, NO production inhibitory effect, lipid peroxidation inhibitory effect, anti-inflammatory effect, cell proliferation effect It is effective to prevent and improve dermatitis.

Hereinafter, the present invention will be described in detail.

The cosmetic composition for preventing and improving dermatitis according to the present invention contains a natural extract mixture formed by mixing a dandelion, mung beans, birch and soruja extract as an active ingredient, the 'natural extract mixture' used in the present invention is dandelion, Not only refers to mixing and extracting mung bean, birch, and sorghum, but also extracting and mixing each of them, and other impurities are included unless they impair the effect of the present invention.

 By containing the natural extract mixture, it is possible to provide a cosmetic composition having an antioxidant effect, an NO production inhibitory effect, a lipid peroxidation inhibitory effect, an anti-inflammatory effect, and a cell proliferation effect which will be described below.

Dandelion ( Taraxacum) used in the present invention platycarpum ) is distributed in Korea, China, and Japan. It is a perennial plant of the dicotyledons, asteraceae, grows in sunny places in the field. In oriental medicine, plants before flowering are used as a medicine called pogongyoung (蒲公英), which is effective for swelling, mastitis, sore throat, appendicitis, peritonitis, acute hepatitis, and jaundice due to heat, and is also used for symptoms of urinating due to fever. In the private sector, it is also used as a drug for quick secretion of milk.

Phaseolus radiatus ) is an annual herb that is a dicotyledonous plant rosemary legume and is also known as Andu and Gildu. It grows well on warm climate loam (black soil with a good mix of sand and clay). Ingredients are starch 53-54%, protein 25-26%, high nutritional value and good flavor. Eat with green beans, mung bean cake, rice cake, green bean tea, mung bean porridge, and host greens. In the private sector, it is used to treat skin diseases and has antipyretic and detoxification effects. The origin is assumed to be India and is mainly distributed in Asia such as Korea, China and India.

Birch (B etula phatyphylla japonica ) is a deciduous arborescent of the dicotyledonous oak tree birch family and grows in the deep mountain. The bark is beautiful and is planted in garden, street and canned trees. Wood is used to make furniture, and in oriental medicine, bark is called baekhwapi (白樺 皮) is used for diuresis, pain, fever. It is distributed in Korea (central north) and Japan.

Rumex ( Rumex crispus ) is a perennial herbaceous genus, 30-80cm high, leaves alternate, oval lanceolate. In June-July, green flowers bloom on the stems of the leaf axes in inflorescences, and the fruit bears achenes. Young leaves are edible. It is found in humid fields, and is distributed in the north of central China.

The natural extract mixture used in the present invention is formed by mixing the dandelion, mung bean, birch, Soruch extract, the natural extract mixture can be extracted by a variety of methods, preferably dandelion, mung beans, birch, snail It is suitable to extract hydrothermally the polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, most preferably by adding ethanol.

In addition, the natural extract mixture formed by mixing the extract of dandelion, mung beans, birch and russetula according to the present invention can be prepared as follows.

 Dandelion, mung beans, birch, and russet are washed with water, and then dried in the shade. Dried dandelion, mung beans, birch trees, and borage are respectively immersed in the extraction container and extracted at an appropriate deposition temperature and deposition time in an appropriate amount of extraction solvent. After this time the extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably ethanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The deposition temperature is 30 ~ 90 ℃, preferably 60 ~ 80 ℃, most preferably maintained at about 70 ℃ is most effective in the prevention of rot, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After obtaining the dandelion, mung bean, birch, sorbus extract, respectively, the solids are removed using a filter paper, the suspension is centrifuged, and the supernatant may be filtered under reduced pressure.

The filtered extract may be concentrated or freeze-dried at 20 to 100 ° C., preferably 50 to 70 ° C., using a vacuum rotary concentrator.

The natural extract mixture used in the present invention is preferably dandelion: mung bean: birch: Sorbus extract is mixed in a weight ratio of 1 to 5: 1 to 2: 1 to 2: 1 to 2 as an active ingredient. Outside this ratio, the effect of preventing or improving dermatitis is lessened.

The natural extract mixture in the composition of the present invention may be contained in 0.005 to 50% by weight relative to the total weight of the composition. When the content of the natural extract mixture is less than 0.005% by weight, the effect of preventing and improving dermatitis due to the inclusion of the natural extract mixture is insignificant, and when it exceeds 50% by weight, problems in formulation stability may occur.

The composition of the present invention may further contain one or more active ingredients exhibiting the same or similar function in addition to the natural extract mixture.

Cosmetics prepared with a cosmetic composition containing the natural extract mixture of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the natural extraction mixture of the present invention, it can be prepared in the form of adjuvants that can be applied topically or systemically as commonly used in the field of dermatology by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition to the natural extract mixtures, the cosmetic compositions of the present invention may further contain fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited thereto.

[ Production Example  1] Preparation of Dandelion Extract

50 g of dandelion was finely crushed in a crusher and immersed in 200 ml of 50% ethanol and warmed in a 70 ° C. water bath for 10 hours or more. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. It was concentrated by lyophilization and dissolved in 10% ethanol to prepare a dandelion extract.

[ Production Example  2] Preparation of Mung Bean Extract

50 g of green beans were finely crushed in a crusher and immersed in 200 ml of 50% ethanol and warmed in a 70 ° C. water bath for 10 hours or more. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. It was concentrated by lyophilization and then dissolved in 10% ethanol to prepare a mung bean extract.

[ Production Example  3] Preparation of Birch Extract

50 g of birch was finely crushed in a crusher, immersed in 200 ml of 50% ethanol, and warmed in a 70 ° C. water bath for 10 hours or more. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. It was concentrated by lyophilization and dissolved in 10% ethanol to prepare a birch extract.

[ Production Example  4] Rumor  Preparation of Extract

50 g of the rubus was finely crushed in a crusher, immersed in 200 ml of 50% ethanol, and warmed in a 70 ° C. water bath for at least 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. It was concentrated by lyophilization and dissolved in 10% ethanol to prepare an extract.

[ Experimental Example  1] Antioxidant Activity Test

In order to confirm the antioxidant activity, it was generally measured using a DPPH radical, which is a method of measuring antioxidant activity. Dandelion extract having a final concentration of 100 ㎍ / ㎖ in the extract extracted in Preparation Examples 1 to 4, and mung bean extract: birch extract: Sorujan yi extract weight ratio of 1: 1: 1, the final concentration of 100 ㎍ / ㎖ extract mixture, dandelion extract: mung bean extract: birch extract: Soryu extract with a weight ratio of 3: 1: 1: 1, the natural extract mixture with a final concentration of 100㎍ / ㎖ and 0.2mM DPPH as an antioxidant solution Each solution was mixed with 100ul and reacted at 37 ° C for 30 minutes, and then the absorbance was measured at 520 nm using an ELISA reader.

Experiment with vitamin C (100 ㎍ / ㎖) known as an antioxidant to compare the relative level of the extract by the following equation (1). As a result of Table 1, the antioxidant power of natural extract mixture was slightly lower than that of Mung bean, birch and Sorbus extract extract, but it was stronger than that of dandelion extract. Could know.

% Antioxidant activity = (1-treated absorbance / untreated absorbance) × 100

Sample Name Vitamin c Dandelion extract Green Beans, Birch, Rumor  Extract mixture Natural extraction  mixture Antioxidant activity (%) 66.2% 38.7% 75.7% 69.8%

[ Experimental Example  2] cell proliferation effect

In order to examine the skin cell proliferation effect of the dandelion extract, mung bean, birch, sol-vu extract and natural extract mixtures formed in the same manner as in Experimental Example 1, normal human fibroblasts were prepared using 96-well micro Each well of the plate (96-well microplate) is inoculated to be 1 × 10 ⁴ cells and incubated for 24 hours in DMEM (Dulbecco's Modified Eagle's Medium) medium. After incubation, the dandelion extract prepared above, mung bean, birch, Sorbus extract mixture and natural extract mixture was replaced with DM medium without serum adjusted to a final concentration of 100 ㎍ / ㎖ and further incubated for 24 hours. After incubation, 10 μl of MTT (3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium: 5 mg / ml) solution was added thereto, and the reaction solution was removed after 4 hours. 100 μl of dimethyl sulfoxide solution was added to each well to dissolve the stained cells, and the absorbance at 570 nm was measured with a microplate reader.

By performing the above procedure, the cell proliferation effect was obtained from Equation 2 below, and the results are shown in Table 2.

Cell proliferation effect (%) = {(absorbance at extract treatment-absorbance of control) / absorbance of control} X 100

Sample Name Dandelion extract Mung Bean, Birch, Soru-Zip Extract Mixture Natural extraction  mixture Cell proliferation effect (%) 24.1% 51.6% 52.3%

     According to the above Table 2, it can be seen that the natural extract mixture has excellent skin cell proliferation effect and has a skin cell regeneration effect.

[ Experimental Example  3] NO  Amount

To investigate the effects of dandelion extract, mung bean, birch, sorbus extract and natural extract mixtures on the production of NO in RAW 264.7 cells induced oxidative stress with LPS, respectively. The test was performed. 8 x 10 ⁵ cells were injected into 24 well plates, and the extracts were treated with 100 µg / ml for 2 hours, followed by LPS (1 µg / ml) for 20 hours. 50 μg of 1% selfanilamide and 50 μg of 0.1% naphthylenediamine dihydrochloride were added to 100 μg of the media supernatant, followed by reaction at room temperature for 10 minutes, and the absorbance was measured at 550 nm. At this time, the standard curve was used to prepare NaNO ₂ for each concentration.

Sample Name extract Untreated group Dandelion extract Green Beans, Birch, Rumor  Extract mixture Natural extraction  mixture NO production amount (μM) 12.1 5.4 8.6 5.8

Through the test, the natural extract mixture was able to inhibit the production of NO far better than the mung bean, birch, Sorui extract extract, it was confirmed that the dandelion extract has a similar NO production inhibitory effect.

[ Experimental Example  4] lipid peroxidation

Determination of TBARS content of the effects of dandelion extract, mung bean, birch, sorbus extract mixture and natural extract mixture on the degree of lipid peroxidation in RAW 264.7 cells induced oxidative stress with LPS in the same manner as in Experiment 1 It was found through. 8 x 10 ⁵ cells were injected into 24 well plates, and the extracts were treated with 100 µg / ml for 2 hours, followed by LPS (1 µg / ml) for 20 hours. 400 μg of TBARS solution was added to the 200 μg medium supernatant, followed by reaction at 95 ° C. for 30 minutes, centrifugation at 4000 rpm for 10 minutes, and the supernatant was taken to measure absorbance at 532 nm. At this time, the standard curve was prepared by preparing 1,1,3,3-TMP (Tetramethoxypropane) by concentration.

Sample Name Dandelion extract Mung Bean, Birch, Soru-Zip Extract Mixture Natural extraction  mixture TBARS IC ₅₀ (µg / ml) 150.7 207.9 148.2

According to Table 4, it was confirmed that when the natural extract mixture was treated, there was an excellent lipid peroxidation inhibitory effect with the mung bean, birch, and thorax extract mixtures, and the dandelion extract had a similar lipid peroxidation inhibitory effect.

[ Production Example  5] Example  And Comparative example

To prepare a cosmetic containing a dandelion extract (Comparative Example 1) and mung bean, birch, Sorbus extract extract (Comparative Example 2) and natural extract mixture (Example 1) formed in the same manner as in Experimental Example 1, To compare with this, a cosmetic composition (Comparative Example 3) lacking an extract was prepared in the following composition ratio.

division Content (% by weight) ingredient Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Natural extraction mixture 2.0 - - - Dandelion extract - 2.0 - - Mung Bean, Birch, Soru-Zip Extract Mixture - - 2.0 - Cetearyl Octanoate 5.0 5.0 5.0 5.0 Isopropyl Palmitate 5.0 5.0 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 2.0 2.0 Polysorbate 60 1.7 1.7 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 1.3 1.3 Spices, preservatives dose dose dose dose Purified water Remaining amount Remaining amount Remaining amount Remaining amount Sum 100 100 100 100

[ Experimental Example  5] anti-inflammatory experiment

In order to examine the anti-inflammatory effect on Preparation Example 5, five ICR mice were divided into five groups. Using the right ear of the ICR mouse as a test site, the ear is washed with ethanol before application of the sample, and 20 μl of the sample is continuously applied once a day for 4 days, and 1 mg of arachidonic acid is applied to the right ear 1 hour after the last application. After 1 hour, the degree of edema of the ear was repeatedly measured three times on both ears with a micrometer. The anti-inflammatory effect was determined based on the arachidonic acid treatment group as shown in Equation 3 below, and the results are shown in Table 6 below. The value is shown by the average value of each group.

Inhibition Rate (%) = (A-B) / A x 100

A: Change of control ear (thickness after sample-thickness of ear before sample treatment)

B: Change of Ear in Sample Treatment Group (Thickness of Ear after Sample Treatment-Ear Thickness Before Sample Treatment)

Ear thickness (㎛) Inhibition rate of inflammation (%) Before sample processing After sample processing Control 253 551 - Example 1 249 329 73.2 Comparative Example 1 257 419 45.6 Comparative Example 2 251 347 67.8 Comparative Example 3 256 540 4.7

       From the results shown in Table 6, it was confirmed that Example 1 has the highest anti-inflammatory effect.

[ Formulation example  1] flexible lotion

     Formulation examples of the flexible lotion containing a natural extract mixture was prepared as shown in Table 7.

Raw material Content (% by weight) Natural extraction mixture 1.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

[ Formulation example  2] nutrition cream

Formulation examples of nutrition cream containing a natural extract mixture was prepared as shown in Table 8.

Raw material Content (% by weight) Natural extraction mixture 2.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Remaining amount

Claims (6)

A cosmetic composition for preventing and improving dermatitis, which contains a natural extract mixture formed by mixing dandelion, mung beans, birch, and rume extract. The method of claim 1, wherein the natural extract mixture, Dandelion: Mung bean: Birch: Sorbus extract extract is a skin composition for preventing and improving dermatitis, characterized in that mixed in a weight ratio of 1 to 5: 1 to 2: 1 to 2: 1 to 2. The cosmetic composition for preventing and improving dermatitis according to claim 1, wherein the composition has an antioxidant effect, an NO production inhibitory effect, and a lipid peroxidation inhibitory effect. The cosmetic composition for preventing and improving dermatitis according to claim 1, wherein the composition has an anti-inflammatory effect and a cell proliferation effect. The cosmetic composition for preventing and improving dermatitis according to claim 1, wherein the natural extract mixture is 0.005 to 50% by weight based on the total weight of the cosmetic composition. According to claim 1, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack , Soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow, characterized in that formed from any one of the formulation group selected from the group consisting of Cosmetic composition for.