KR102223368B1 - Cosmetic composition for skin - Google Patents

Abstract

As an active ingredient, it relates to a cosmetic composition comprising a golden extract, a licorice extract, a gosam extract, a white sesame extract, and a hwangbaek extract, and has anti-wrinkle, whitening, antioxidant and anti-inflammatory activities.

Description

Cosmetic composition for skin}

The present invention relates to a cosmetic composition comprising golden extract, licorice extract, gosam extract, baekseonpi extract and hwangbaek extract as active ingredients, and has anti-wrinkle, whitening, antioxidant and anti-inflammatory activities.

It is known that the skin is wrinkled due to a deficiency of collagen, the skin collagen. Collagen is a major protein constituting the skin dermis and plays a role in maintaining skin structure and elasticity. As the synthesis of collagen decreases with age, the dermal layer of the skin is depressed and wrinkles are created.

Tyrosinase is an enzyme present in most plant and animal tissues, including mushrooms, potatoes, and apples, and is an enzyme involved in the most important early rate-determining step in the melanin biosynthesis pathway in the human body. Many whitening ingredients have a mechanism of action that inhibits the tyrosinase enzyme.

For the purpose of skin health or beauty, it has anti-inflammatory and antioxidant effects, as well as increasing collagen synthesis or inhibiting tyrosinase activity, so it is required to find a component that improves skin wrinkles and has a whitening effect.

An example of the present invention aims to provide a cosmetic composition comprising a golden extract, a licorice extract, a gosam extract, a baekseonpi extract, and a hwangbaek extract as an active ingredient.

The present invention completes the present invention by confirming that it is possible to prepare a cosmetic composition having anti-wrinkle and whitening activities as well as anti-oxidation and anti-inflammatory properties when using golden, licorice, and white ginseng known as traditional herbal medicines as active ingredients. I did. Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition comprising a golden extract, a licorice extract, a gosam extract, a baekseonpi extract, and a hwangbaek extract as an active ingredient.

Golden ( Scutellaria baicalensis ) is a dicotyledonous plant, a perennial herb of the Lamiaceae family, and is distributed in Korea, China, Mongolia, and eastern Siberia. In the conventional oriental medicine, the root of gold was used as an antipyretic, diuretic, branch, and otodam agent.

Liriope (Broadleaf Liriope, Liriope) platyphylla ) is a perennial plant of the lily family Liliaceae, a monocotyledonous plant, and is widely distributed not only in Korea, Japan, China, and Taiwan, Northeast Asia, but also worldwide. It is the top of the ovary, and the fruit is round with capsules, and the skin is peeled off early, so the seeds are exposed and the color is purple. In conventional oriental medicine, tuber root was used as a medicinal material, but it was used as a tonic, jinhae, expectorant, and strong heart.

Sophora flavescens is a perennial plant of the dicotyledonous rosewood leguminous family, and is also known as a thief's wand, a red ginseng, and a snake's spermatozoa. In conventional oriental medicine, dried roots are called gosam, and it is known to be prescribed for indigestion, neuralgia, hepatitis, jaundice, and hemorrhoids due to its taste and efficacy of ginseng. In the private sector, stems and leaves were also used as insecticides.

Ringworm blood is ringworm of the family of Woonhyang ( Dictamnus dasycarpus Turcz . ), and used for skin rash due to moist heat, skin erosion, eczema, rubella, itching and jaundice due to acute hepatitis, moist heat limb paralysis, and also used in seawater, throat dryness, and alternately. As a pharmacological action, it has been known to have antipyretic action and suppression of skin fungi.

Hwangbaek is the dried bark of the rutaceae family. The bark is peeled off the trunk of the tree before and after the lower limbs to remove or chop and dry in the sun. Hwangbaek is known to reduce blood sugar, inhibit the growth of pneumococcal, tuberculosis, and staphylococcus, and inhibit the reproduction of tumor cells and sterilize them. In addition, it is known to promote the secretion of gastric juice when taken and to lead to an increase in appetite.

The licorice extract may contain 50 to 150 parts by weight, specifically 80 to 120 parts by weight, more specifically 90 to 110 parts by weight, for example, 100 parts by weight, based on 100 parts by weight of the golden extract.

The Gosam extract may include 150 to 250 parts by weight, specifically 180 to 220 parts by weight, more specifically 190 to 210 parts by weight, for example, 200 parts by weight of the Gosam extract based on 100 parts by weight of the golden extract.

The Baekseonpi extract may contain 50 to 150 parts by weight, specifically 80 to 120 parts by weight, more specifically 90 to 110 parts by weight, for example, 100 parts by weight, based on 100 parts by weight of the golden extract. .

The Hwangbaek extract may include 50 to 150 parts by weight, specifically 80 to 120 parts by weight, more specifically 90 to 110 parts by weight, for example, 100 parts by weight, of the Hwangbaek extract based on 100 parts by weight of the golden extract.

Specifically, based on 100 parts by weight of the golden extract in the cosmetic composition, it may include 50 to 150 parts by weight of a licorice extract, 150 to 250 parts by weight of a gosam extract, 50 to 150 parts by weight of a tinea extract, and 50 to 150 parts by weight of a yellow white extract have.

For example, each extract of the cosmetic composition may be an extract extracted from one or more solvents selected from the group consisting of water, organic solvents, and mixtures thereof. The solvent may be, for example, butylene glycol.

The extraction may be, for example, a liquid obtained by chilling, heating, or filtering at room temperature, or may be obtained by evaporating, spray drying, or freeze-drying a solvent from the liquid, but is not limited thereto.

The cosmetic composition may contain a cosmetically acceptable carrier, for example, a fatty substance, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, and lipid vesicles One or more selected from among may be included, and any ingredient that can be used in cosmetics may be included without being limited thereto.

For example, the carrier is animal fat, vegetable fat, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, lactose, talc, silica, aluminum hydroxide, calcium As silicate, polyamide powder, chlorofluorohardcarbon, propane/butane dimethyl ether, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil Cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol, sorbitan fatty acid ester, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, and one or more carriers selected from the group consisting of tracanite may be included, but not limited to the formulation or necessity.

The cosmetic composition is provided in the form of a solution, gel, solid, anhydrous product of dough, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule, an ionic (liposome), and a nonionic vesicle dispersant. Can be. Specifically, flexible lotion, astringent lotion, nutrition lotion, nutrition lotion, nutrition essence, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, gel, powder, makeup base, It may be a formulation selected from the group consisting of body lotion, body cream, body oil, body essence, lotion, ointment, gel, cream, patch, and spray, but is not limited thereto.

The cosmetic composition has an antioxidant effect, and may be an antioxidant cosmetic composition. Specifically, the cosmetic composition may be characterized by having DPPH scavenging ability.

The cosmetic composition has an anti-inflammatory effect and can be used as an anti-inflammatory cosmetic composition, and specifically, it may be characterized in that it inhibits NO synthesis. It can be used to improve skin irritation or trouble.

In addition, the cosmetic composition may be a cosmetic composition for promoting collagen synthesis, enhancing skin elasticity or anti-wrinkle. Specifically, the cosmetic composition is characterized in that the collagen synthesis ability is improved, and the collagen synthesis ability is improved to increase the generation of wrinkles, thereby improving skin elasticity. For example, the composition may be characterized in that the increase rate of collagen synthesis is 130 to 250%, specifically 150 to 210%, and more specifically 170 to 200%, compared to 100% of the negative control without sample treatment.

Since the composition has a whitening effect, it can be used as a cosmetic composition for whitening, and specifically, it may be characterized by inhibiting the activity of tyrosinase.

As another example of the present invention, it provides a pharmaceutical composition for preventing or treating skin aging, comprising a golden extract, a licorice extract, a gosam extract, a baekseonpi extract, and a hwangbaek extract as active ingredients.

Details of the cosmetic composition may be equally applied to the pharmaceutical composition.

The composition of the present invention not only has excellent antioxidant and anti-inflammatory effects, but also has anti-wrinkle, anti-wrinkle and whitening effects, and thus can be usefully used as an active ingredient in skin cosmetics.

1A to 4B are views exemplarily showing experimental results according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail by specific examples. However, the present invention is not limited to the following examples, and it is apparent to those skilled in the art that various modifications or modifications are possible within the spirit and scope of the present invention. Accordingly, the appended claims should be broadly interpreted in accordance with the spirit and scope of the present invention.

Example 1. Preparation of pharmaceutical extract

Each of the gold, maekmundong, gosam, baekseonpi, and hwangbaek was heated in a mixture of butylene glycol and water at 95° C. for 1 hour and 30 minutes, stirred, and then filtered under reduced pressure through a filter paper. The filtered extract was refrigerated at a temperature of 4° C. for 24 hours, and then filtered under reduced pressure to obtain an extract, which was stored refrigerated at a temperature of 4° C.

Example 2. Preparation of cosmetic composition

The extract prepared in Example 1 was weighed according to the weight shown in Table 1 below, and the mixture was stirred and then filtered under reduced pressure with a filter paper to prepare a cosmetic composition. As shown in Table 1, Comparative Example 1, which did not contain any extract, and Comparative Examples 2 and 3 in which the composition ratio of each extract was different were used as controls.

Gold Maekmundong Gosam Ringworm Yellow white Example 2 100g 100g 200g 100g 100g Comparative Example 1 - - - - - Comparative Example 2 200g 100g 200g 100g 100g Comparative Example 3 200g 100g 200g 100g 100g

Example 3. Collagen Synthesis ability evaluation

Fibroblasts in the dermal layer were used to measure the degree of increase in the synthesis of collagen, a major protein in the skin.

After dispensing normal human dermal fibroblast (NHDF) into 96 wells, the compositions of Example 2 and Comparative Examples 1 to 4 were added and cultured _{in a CO 2 incubator.} After treatment with the antibody-POD conjugate solution of the Procollagen PIP EIA kit, it was cultured. After removing the culture medium and washing with PBS, the prepared sample was measured for absorbance, and the rate of increase in collagen synthesis was calculated as a relative value based on 100% of the synthesis ability of Comparative Example 1 without processing the sample. The results are shown in Table 2 below and FIGS. 1A to 1E.

division Collagen synthesis increase rate (%) Example 2 184 Comparative Example 1 100 Comparative Example 2 126 Comparative Example 3 161 Comparative Example 4 145

As can be seen from the above results, the composition of Example 2 not only has remarkably excellent collagen synthesis ability compared to Comparative Example 1, which is a negative control, but also has excellent collagen synthesis effect compared to Comparative Examples 2 and 3 having a similar composition or different mixing ratio. It was confirmed that the wrinkle effect was excellent.

Example 4. Evaluation of antioxidant activity according to DPPH scavenging ability

DPPH (1,1,-diphenyl-2picrylhydrazyl radical) is a reagent that can measure the reducing power of antioxidants because it exists as a radical with a stabilized structure centered on nitrogen in the compound. Using DPPH, the antioxidant effect of the composition of Example 2 was confirmed. Ascorbic acid, known as a representative antioxidant, was used as a positive control.

To 0.5ml of 0.2mM DPPH methanol solution, 1ml of sample solution of 6.25vv%, 12.5v/v%, 25v/v%, 50v/v% concentration was added, mixed, reacted at room temperature for 30 minutes, and reduced absorbance at 517 nm. It was measured. The size of the reducing power of the sample is expressed as the radical scavenging activity (SC ₅₀ ), and the SC ₅₀ indicates the concentration of the sample required to reduce the concentration of DPPH by 50%. The measurement results of the antioxidant activity of Example 2 are shown in Fig. 2A, and the measurement results of the positive control group are shown in Fig. 2B, respectively. As can be seen from the results, it was confirmed that the composition of Example 2 had an antioxidant effect similar to that of ascorbic acid.

In addition, it was confirmed that antioxidant activity also increased with increasing concentration. In particular, it was confirmed that it has a high antioxidant activity compared to the concentration at 6.25v/v%.

Example 5. Evaluation of NO synthesis inhibitory ability

NO is a pathological secondary signaling substance secreted by many cells. It regulates the activity of COX and acts synergistically on the inflammatory response. In Raw264.7 cells, NO generated from NOS induced by LPS is measured and inflammation The effect of inhibiting the reaction was confirmed.

After treating the composition of Example 2 on Raw264.7 cells, the NO synthesis inhibitory ability was evaluated using the NO quantification kit based on the following equation (1). The control group was treated with only LPS without adding a sample. The results are shown in FIG. 3.

[Equation 1]

NO synthesis inhibitory ability (%) = {1-(Slope of NO synthesis amount when sample is added)}/(NO synthesis amount when sample is not added) * 100

From the results, it can be seen that the composition of Example 2 according to the present invention has an anti-inflammatory effect.

Example 6. Evaluation of tyrosinase inhibitory activity

Tyrosinase is an enzyme involved in the most important early rate-determining step in the melanin biosynthesis pathway in the human body, and can be seen to have whitening activity when tyrosinase is inhibited. The whitening effect of the composition of the present invention was evaluated by measuring the inhibitory effect of tyrosinase on the DOPA oxidation reaction.

Example 2 The composition treatment results are shown in Fig. 4a, and the positive control kojic acid treatment results are shown in Fig. 4b, respectively. It was confirmed that the composition of Example 2 had whitening activity by inhibiting tyrosinase in a concentration-dependent manner. In particular, it was confirmed that the composition had a whitening effect similar to that of the positive control kojic acid, and specifically, the composition of Example 2 had a whitening effect corresponding to 10.2 μg/ml of kojic acid.

Claims (9)

It contains golden extract, licorice extract, gosam extract, white sesame extract and hwangbaek extract as active ingredients,
Based on 100 parts by weight of the golden extract, for enhancing skin elasticity or anti-wrinkle, including 80 to 120 parts by weight of Macmundong extract, 180 to 220 parts by weight of Gosam extract, 80 to 120 parts by weight of Baekseonpi extract and 80 to 120 parts by weight of Hwangbaek extract Cosmetic composition. The cosmetic composition of claim 1, wherein the extract is at least one extract selected from the group consisting of water, organic solvents, and mixtures thereof. The cosmetic composition of claim 1, wherein the extract is a liquid obtained by chilling, heating, or filtering at room temperature, or by evaporating, spray drying, or freeze-drying a solvent from the liquid. The method of claim 1, wherein the cosmetic composition is a softening lotion, astringent lotion, nourishing lotion, nourishing lotion, nourishing essence, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, A cosmetic composition, a formulation selected from the group consisting of gel, powder, makeup base, body lotion, body cream, body oil, body essence, lotion, gel, cream, patch, and spray. delete delete delete delete delete

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