KR20170136378A - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition which contains composite medicinal herb extracts as active ingredients, excellently improves skin condition, and is safe to be applied to skin. More specifically, the composition, according to the present invention, contains a Korean angelica root extract, a white atractylis extract, an Asparagus cochinchinensis Merr. extract, a liriope rhizome extract, a peach kernel extract, a Rehmanniae Radix Preparata extract, a milk vetch root extract, a dictamnus root extract, a safflower extract, a scutellaria extract, a Cimicifuga heracleifolia Komarov extract, a larch agaric extract, a licorice extract, and a moutan extract as the active ingredients, thereby bringing outstanding skin condition improvement effects such as anti-saccharification effects, skin-whitening effects, skin elasticity and wrinkle amelioration effects, antioxidant effects, and alleviation effects for troubled skin.

Description

[0001] The present invention relates to a composition for improving skin comprising a herbal extract,

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect.

The skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control function, excretion function, which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of skin cells may deteriorate and the skin condition may deteriorate.

Collagen and elastin present in the dermis of the skin perform functions such as mechanical rigidity of the skin, resistance of the connective tissue, maintenance of the cohesion of the tissue, and support of the cell adhesion, and have a great influence on the constituents of the skin. It is known that such collagen is reduced by photoaging due to stress, aging, or ultraviolet irradiation, while elastin is known to be distorted in three-dimensional structure by the enzymatic degradation enzyme Elastase after exposure to ultraviolet light. As a result, the skin tissue is loosened and the elasticity is lost.

When the cell function of the epidermis is lowered, the metabolism is not smooth and the exfoliation does not occur well, and when the ultraviolet rays are received in a state where the keratin is overly accumulated, the elasticity is lowered and wrinkles are generated. If the skin is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin becomes dry and wrinkles are formed.

When the skin receives ultraviolet rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through the melanoma. This keratinocyte turns over over a period of 28 days. Therefore, the melanin produced is generally lost by the keratinocyte periodically for 28 days. However, when the skin cell cycle is not properly controlled by the stress, skin aging, etc., keratinocytes do not fall out in the presentation period and the pigment such as spots, freckles, Calm occurs.

In the skin, sebum, sweat and cosmetic ingredients are decomposed into substances that are toxic to the skin by the fungus, which can cause skin irritation and inflammation. In addition, skin irritation caused by ultraviolet rays increases the production of nitrogen monoxide (NO), which is an inflammation mediator, to cause skin troubles. The production of nitrogen monoxide is mostly caused by iNOS, and it is known that iNOS is rapidly induced by stimulation such as LPS and cytokine to produce excessive NO.

A reactive oxygen species (ROS), also known as a toxic oxygen species, is a cell-generated toxic substance produced by physiological actions such as respiration and is constantly produced and extinguished, with 3-5% in normal conditions . These reactive oxygen species are free radicals such as superoxide radicals (O2-) and hydroxyl radicals (HO +), which are chemically bound to atoms or molecules that are not paired with the outermost electron orbits Highly unstable and highly reactive) or in the form of compounds with paired electrons such as hydrogen peroxide (H ₂ O ₂ ) or singlet radicals. Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. It has been reported that these reactive oxygen species attack biological molecules and damage cells and tissues and cause various diseases related to aging and various diseases.

Research has been continued to suppress the skin aging caused by various factors as described above, to improve whitening, wrinkles, elasticity or trouble, and to develop a substance having an antioxidative effect.

Materials having the above-mentioned effects are widely distributed in the natural world, and they have been mainly used as raw materials for foods, cosmetics, medicines and the like using materials derived from plants. However, the substances derived from such natural substances are not effective enough to be used in large quantities in order to obtain a meaningful effect, resulting in toxicity and price increase.

In order to solve the problems of such natural materials, the development of chemically synthesized materials has continued. Although they have a merit that they exert a superior effect even when used in a small amount compared to a natural substance, their use is limited due to a fatal problem that can cause large and small side effects to the human body.

Accordingly, it is inevitable to develop a substance derived from a natural product and having stability, while exhibiting an excellent effect on skin improvement.

KR 1020060092179 A KR 1020090002682 A

A problem to be solved by the present invention is to provide a complex herbal medicine extract which is derived from natural materials and has stability, while exhibiting excellent effects on skin, in particular whitening, wrinkle, elasticity, trouble improvement and antioxidation.

In order to solve the above-mentioned problems, the present invention provides a method for the treatment and / or prevention of diseases such as angelica gigantosa, extracts of Chrysanthemum gigantis, extracts of Chunmunmdong, extracts of mungmundong, extracts of doenjang, (For example, a cosmetic composition) for skin whitening, wrinkle improvement, elasticity improvement, trouble-improving or antioxidation, which comprises an extract of Rootini as an active ingredient.

[extract]

Angelica Gigas belongs to the persimmon, and contains the roots of Angelica Gigas Nakai . It has the property to make the body and the body healthy. The body of Angelica Gigas regulates the blood and the upper part of the Angelica gigas Has the effect of making blood.

Atractylodis Rhizoma Alba is an extract of Atractylodes japonica Koidzumi ex Kitamura or Atractylodes macrocephala Koidzumi (Chrysanthemum and Compositae) rootstock as it is or has been removed. It has a peculiar smell, taste a little bit weary, chewy, viscous and warm.

Asparagus cochinchinensis Merrill is a perennial herbaceous plant belonging to the Liliaceae. It grows in the southern coastal province of Korea. It is a root of roots, which is either boiled with hot water or steamed, then removed from the surface and dried.

Macmun-dong is a perennial herbaceous plant belonging to the Liliaceae, Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawler) is the bulge of the root.

Touin (桃仁) is a Rosaceae (Rosaceae) peach tree ( Prunus Persica Batsch or Prunus davidiana Franchet.

熟地 黄 (熟地黄) is the root of the scrophulariaceae ( Rehmannia glutinosa (Gaertner) Liboschitz ex Steudel) is steamed and dried, darkens the hair, is said to be effective in the recovery of fatigue.

Hwanggi (皇 耆) is a perennial herb that belongs to leguminosae ( Astragalus membranceus Bunge), 蒙古黄gi ( Astragalus membranaceus There is a bungee. mongholicus Hsiao), or as a root.

White pine bark is the roots of white pine ( Dictamnus dasycarpus Turczaininov), a perennial plant of rutaceae .

Safflower (safflower,Carthamus tinctorius) Is a biennial herbaceous plant belonging to CompositaeCarthamus tinctorius Linne) is a flower.

The golden (黄 芩, Scutellariae Radix) Scutellaria baicalensis Georgi belongs to the Labiatae, and is either removed as a root or removed. The roots are conical, twisted and curved, and the outer surface is tan to dark yellow. The vagina is firm, vulnerable and easy to cut. The cut surface is yellow and the center is reddish brown. There is little smell and taste is a little bit. It is distributed in Korea, China, Mongolia and eastern Siberia.

Rimoma (Cimicifugae Rhizoma) is a perennial herb of Ranunculaceae that grows in forests. Cimicifuga heracleifolia Komarov, Cimicifuga simplex Wormskjord, Cimicifuga dahurica Maximowicz) or stork horse (Cimicifuga foetida Linne).

Poria cocos Wolf is a sclerotia of Polyporaceae and is known to have anticancer action.

Licorice (licorice, Glycyrrhizae Radix) is a perennial herb belonging to leguminosae, licorice ( Glycyrrhiza uralensis Fischer), light and licorice ( Glycyrrhiza glabra Linne) or licorice (licorice licorice, Glycyrrhiza inflata Batal) as root or rootstock.

Mokdanpi (牧丹皮) is the peony (Paeoniaceae) Peony (Paeonia suffruticosa Andrews).

The present inventors have found that all of the above extracts have a biological activity beneficial to the skin, and the complex extract of two or more of the above extracts, preferably Angelica keiskei extract, Chrysanthemum extract, Chrysanthemum root extract, , A safflower extract, a golden extract, a horse extract, a ginseng extract, a licorice extract and a ginseng extract were found to have synergistic biological activity.

In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.

Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.

In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be subjected to a drying process (for example, drying at 40 to 70 DEG C for 15 to 50 hours) before pulverization and then pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.

The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.

[Composition]

The composition according to the present invention is effective for extracting Angelica gigas Nakai, extracts of Chrysanthemum morbus, extracts of Chunmunmundo, extracts of mungmundong, extracts of Doenjang, Seokgwangsu, Hwanggi, As an ingredient.

In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.

Each plant extract may be included in the composition according to the present invention in the following proportions. For example, the weight ratio of Angelica keiskei L.: Root Extract: Chungmun Dong Extract: Mukmun Dong Extract: Dane Extract: Sukjiophora Extract: Hwanggi Extract: White Rice Extract: Safflower Extract: 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 to 10 0.1 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 5: 0.1 to 5: 0.1 to 5, 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3, 1: 0.1 to 2: 0.1 to 2: 0.1 to 2: 1: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 ~ 1.5: 0.5 ~ 1.5: 0.5 ~ 1 0.5: 1.5 to 0.5: 1.5 to 0.5: 0.5 to 1.5.

The compositions according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012% , 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026% %, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% 0.005%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0058% , 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077% %, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095% 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275 %, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650% 0.0725%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000% , 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250 %, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0% 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9% , 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2% 5.4%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2% 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7% , 7.9%, 8. 9.2%, 9.4%, 9.4%, 9.5%, 9.6%, 8.3%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0% , 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21% %, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75% 85%, 90%, 95%, or 99% or more of the extract of the present invention. The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.

The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.

The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.

The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.

In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.

May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.

The composition according to the present invention may be provided as a topical skin composition.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.

As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.

In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.

The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.

The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.

The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .

As a result of treating the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And vitamin C, and the effect of quercetin, which is known to inhibit the activity of the skin elastase, is exerted. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkle and elasticity improvement effect.

Further, the composition according to the present invention has an effect of improving the trouble.

The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.

As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.

In addition, the composition according to the present invention has an antioxidative effect.

The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.

As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.

In addition, the composition according to the present invention has an antiherating effect.

The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.

As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.

The complex extract according to the present invention is safe for the skin and has an excellent effect for improving the skin including anti-glycation effect, skin whitening effect, skin elasticity and wrinkle improving effect, antioxidative effect and skin trouble improving effect.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

< Example  1> Preparation of Angelica gigantosa extract

The Angelica gigas was dried well and cut into three pieces. 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 탆 to prepare Angelica giganta Radix extract.

< Example  2> Preparation of extract

After thoroughly drying the flask, the flask was charged with 100 g of dry weight and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a crude extract.

< Example  3> Chunmun Dong  Preparation of extract

After the wells were well dried, 100 g of dry weight was added to the flask and extracted with 1000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare an astragmons dong extract.

< Example  4> Manufacture of Macromolecular Extract

After drying thoroughly, 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mulberry extract.

< Example  5> Preparation of Diane Extract

After thoroughly drying and finely grinding, 100 g of dry weight was put into a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a dried extract.

< Example  6> Preparation of Sukjipu Extract

After finely drying, 3 g of dried ginger was added to a flask and 100 g of dry weight was extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a watery extract.

< Example  7> Preparation of Hwanggi Extract

The dried yellow oak was thoroughly dried, and 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 mu m to prepare an extract of hwanggi.

< Example  8> White line blood  Preparation of extract

The white blood was well-dried, and after three-fold drying, 100 g of dry weight was added to the flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a white line extract.

< Example  9> Production of safflower extract

After drying the safflower well, it was diluted three times with 100 g of dry weight in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a safflower extract.

< Example  10> Preparation of gold extract

After the gold was dried well, the dried weight of 100 g was put into a flask and extracted with 1000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a golden extract.

< Example  11> Manufacture of horse riding extract

The riding was well dried and cut into three pieces, and 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a horse riding extract.

< Example  12> Manufacture of ginseng extract

After thoroughly drying the bamboo shoots, the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a bamboo shoot extract.

< Example  13> Preparation of licorice extract

The licorice was dried well, and the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a licorice extract.

< Example  14> Preparation of Root Extract

After thoroughly drying the slices, the dried weight of 100 g was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a herringbone extract.

< Example  However, Chunmun Dong , McMundoong, Doin, Sukjihwanghwang, Hwanggi, White line blood , Safflower, golden, horse riding, bokyeong, licorice, and rhizome (14 kinds) mixed extracts

In the same manner as in the above-mentioned examples, the same amounts of the extracts of Angelica gigas Nakai, Baekwoo, Chunmundong, Maekmundong, Doin, Seokgi, Hwanggi, , Mangmundong, doein, saengjiwanghwang, hwanggi, white pine bark, safflower, golden, horse riding, bokyeong, licorice and mulberry.

< Experimental Example  1> Antialcification  effect

In order to confirm anti-glycation efficacy, the present inventors measured glycosylation inhibitory activity using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were dissolved by using 1M phosphate buffer (pH 7.4), and prepared by diluting the sample to 50ppm with 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 [mu] l each was added to a 96-well plate. To each sample, 100 μl of 0.01 M aminoguanidine was added to each sample to be diluted to 50 ppm and used as a positive control. These samples were mixed well and finally glucose diluted with 1M phosphate buffer solution was added to the final concentration of glucose to 0.1 M and reacted at 70 ° C for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

Glycation group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. Absorbance was measured at 420 nm by adding 1M L-arginine and sample alone without glucose to measure the absorbance of the sample itself. The saccharide inhibitory activity can be obtained by the following formula. Experiments were performed three times each and expressed as a mean value.

[Equation 1]

('Glycation test group' in the above equation (1) means 'Absorbance of Glycation test group')

sample Inhibition rate (%) The control (DMSO, 50 ppm) - The positive control (Aminoguanidine, 55 ppm) 54.89 Example 1 42.31 Example 2 45.35 Example 3 48.37 Example 4 36.29 Example 5 27.77 Example 6 41.32 Example 7 51.22 Example 8 35.26 Example 9 31.86 Example 10 39.69 Example 11 47.28 Example 12 48.33 Example 13 50.68 Example 14 37.84 Example 15 (50 ppm) 67.69

As can be seen from the results shown in Table 1, the mixed extract showed better anticarcinogenic effect than aminoguanidine, which is known as an anticarcinogenic substance.

< Experimental Example  2> Melanin formation inhibitory effect

In order to confirm the whitening effect through inhibition of melanin formation, a culture solution of B-16 mouse melanoma cells in rat was cultured according to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980) The total amount of melanin was measured by adding an extract. In the experiment, first the toxicity of melanoma cells in rats was evaluated and the whitening evaluation was carried out at a concentration that is not toxic. DMSO was used as a negative control group, and albutin was used as a positive control group.

Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to be 100 ppm, and melanoma cells were cultured for 3 days. Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as the inhibition rate (%). The results are shown in Table 2 below. As shown in FIG.

sample Melanin production
(abs) Inhibition rate (%) Control group (DMSO, 10 ppm) 0.33 - Positive control (arbutin, 100 ppm) 0.228 30.91 Example 1 0.318 3.64 Example 2 0.290 12.12 Example 3 0.302 8.48 Example 4 0.298 9.70 Example 5 0.311 5.76 Example 6 0.301 8.79 Example 7 0.288 12.73 Example 8 0.273 17.27 Example 9 0.311 5.76 Example 10 0.297 10.00 Example 11 0.273 17.27 Example 12 0.253 23.33 Example 13 0.297 10.00 Example 14 0.285 13.64 Example 15
(100 ppm) 0.230 30.30

As can be seen from the results of Table 2 above, the mixed extract showed excellent melanin total amount reduction effect, which is useful for whitening purposes.

< Experimental Example  3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and the improvement of skin trouble, nitric oxide (NO) production inhibition experiment was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates so as to enter 3x10 ⁵ wells into each well, and cultured for 24 hours. Subsequently, the cell culture medium containing the sample was replaced with a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of GRIESS solution was added thereto, and the reaction was allowed to proceed at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect. Calculated using Equation (2) and shown in Table 3 below. Experiments were performed three times each and expressed as average values.

&Quot; (2) &quot;

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

sample NO production inhibition rate (%) Control group (DMSO, 10 ppm) - L-NMMA (positive control, 10 ppm) 41.02 Example 1 26.96 Example 2 25.39 Example 3 27.99 Example 4 30.15 Example 5 34.29 Example 6 26.42 Example 7 24.44 Example 8 34.21 Example 9 38.45 Example 10 36.41 Example 11 29.88 Example 12 32.17 Example 13 28.69 Example 14 34.15 Example 15
(10 ppm) 39.98

As can be seen from the results in Table 3, the mixed extracts showed a relatively low relative activity but excellent activity as a natural substance when compared with L-NMMA, a representative anti-inflammatory drug.

< Experimental Example  4> Promoting collagen synthesis

The sample was added to the culture medium of human - derived fibroblasts to confirm the promoting effect of type I collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay Kit). In order to measure the amount of collagen synthesis, the sample was added to a fibroblast culture medium (DMEM medium) at a final concentration of 10 ppm and cultured for 48 hours. The culture broth was taken and the degree of type 1 collagen synthesis was measured at each concentration using a PICP EIA kit And measured at 450 nm using a spectrophotometer.

For the comparison of the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium of the untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 / / ml. The increase rate of collagen production was calculated by the ratio of relative collagen production to the negative control, and the results are shown in Table 4 below.

sample Type 1 collagen production (ng / ml) Growth rate (%) Negative control group 150.2 - Positive control (vitamin C) 246.8 64.3 Example 1 200.7 33.6 Example 2 168.3 12.1 Example 3 218.5 19.4 Example 4 176.9 17.8 Example 5 194.3 29.4 Example 6 179.2 19.3 Example 7 198.9 32.4 Example 8 213.7 42.3 Example 9 217.3 44.7 Example 10 221.1 47.2 Example 11 184.9 23.1 Example 12 209.7 39.6 Example 13 209.5 39.5 Example 14 188.0 25.2 Example 15 (10 ppm) 246.3 64.0

As can be seen from the results in Table 4, the collagen synthesis was increased when the mixed extract was treated, and the collagen synthesis effect was similar to that of vitamin C, which is generally known to induce collagen synthesis.

< Experimental Example  5> Ella Stays  Active inhibitory effect

The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastinase was diluted with a buffer solution to make a final concentration of 0.5 mM after making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an inhibitor of Elastinase. Ellazease inhibition candidates were added to give a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of the absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.

&Quot; (3) &quot;

The inhibition rate of the ELA stase was calculated using the above equation (3) and shown in the following Table 5, and the experiment was performed three times each and expressed as an average value.

sample Enzyme activity Inhibition rate (%) Example 1 4.7 54.80 Example 2 4.9 52.88 Example 3 4.3 58.65 Example 4 5.1 50.96 Example 5 3.7 64.42 Example 6 5.5 47.12 Example 7 8.1 22.12 Example 8 5.7 45.19 Example 9 4.2 59.62 Example 10 6.0 42.31 Example 11 7.9 24.04 Example 12 5.2 50.00 Example 13 4.4 57.69 Example 14 6.5 37.50 Example 15
(10 ppm) 3.6 65.38 The positive control (Quercetin, 10 ppm) 3.5 66.35 The control (DMSO, 20 ppm) 10.4 -

As can be seen from the results of Table 5, when the mixed extract was treated, it was found that Elastase activity inhibitory effect excelling even in comparison with the positive control group, quercetin, was exerted. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

< Experimental Example  6> Antioxidant effect

The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of mixed extract or vitamin C, a positive control, was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

IC ₅₀ represents the concentration of the extract when the absorbance of the sample is half the absorbance of the control group. The results are shown in Table 6 below. This experiment was repeated 3 times.

sample IC50 (% w / v) Control group 0.0025 Positive control (vitamin C) concentration 0.0005 Example 1 Concentration 0.0007 Example 2 Concentration 0.0009 Example 3 Concentration 0.0022 Example 4 Concentration 0.0017 Example 5 Concentration 0.0011 Example 6 Concentration 0.0013 Example 7 Concentration 0.0023 Example 8 Concentration 0.0012 Example 9 Concentration 0.0015 Example 10 Concentration 0.0010 Example 11 Concentration 0.0021 Example 12 Concentration 0.0014 Example 13 Concentration 0.0024 Example 14 Concentration 0.0019 Example 15 Concentration 0.0006

As shown in Table 6, the mixed extracts showed a strong free radical scavenging ability as compared with vitamin C, confirming that the antioxidative effect was excellent.

Claims (4)

Skin whitening, which contains as an active ingredient, an effective ingredient, Angelica keiskei extract, Lycopersicon esculentum extract, Astragalus koreanum extract, Maekmoongdong extract, Diane extract, Sukjiophora extract, Hwanggi extract, White radish extract, Safflower extract, Golden extract, , Wrinkle improvement, elasticity improvement, trouble improvement, or antioxidation. The method according to claim 1, wherein the extract of Angelica keiskei is selected from the group consisting of Angelica keiskei keiskei extract, Angelica keiskei kusatake extract, 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10 : 0.1 to 10. 3. The cosmetic composition according to claim 1 or 2, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohols or a mixture thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition is antialcification, inhibition of melanin formation, promotion of collagen synthesis, inhibition of elastase activity or free radical scavenging.