KR102612017B1 - Composition for skin improvement comprising multiple extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing mixed extracts of rose, peony, and iris as active ingredients, and more specifically, to a cosmetic composition with excellent effects such as skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory properties. It relates to composition. The composition of the present invention has excellent anti-inflammatory and antioxidant effects, reduces the total amount of melanin, promotes collagen synthesis, and has an excellent moisturizing effect, so it can be used as a cosmetic composition for whitening skin, improving elasticity or improving wrinkles, and moisturizing the skin. You can. Additionally, the composition containing the mixed extract of rose, peony and iris of the present invention can be used as a functional food composition and quasi-drug composition.

Description

Cosmetic composition comprising mixed extract {Composition for skin improvement comprising multiple extract}

The present invention relates to a cosmetic composition containing mixed extracts of rose, peony and iris, and more specifically, to a cosmetic composition containing the mixed extract as an active ingredient for skin whitening, skin elasticity improvement, wrinkle improvement, skin moisturizing, antioxidant or anti-inflammatory, etc. It relates to a cosmetic composition with excellent effects. Additionally, the present invention relates to a functional food composition and a quasi-drug composition containing mixed extracts of rose, peony, and iris.

Commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C (L-Ascorbic acid) or their derivatives, and various plant extracts. There is. By inhibiting the synthesis of melanin pigment, they can not only brighten skin tone and achieve skin whitening, but also improve skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, or genetics. However, when applied to the skin, there is a limit to the amount of use due to safety issues such as irritation and redness, or the effect is so small that no practical effect can be expected.

Collagen is a major matrix protein produced by skin fibroblasts and is present in the extracellular matrix. Its important functions include mechanical rigidity of the skin, connective tissue resistance and tissue adhesion, support for cell adhesion, and cell division and differentiation. Induction (during the growth of organisms or wound healing) is known. Collagen decreases due to age and photoaging caused by ultraviolet irradiation, and collagen reduction is promoted by collagenase enzyme activity that decomposes collagen. This is known to be closely related to the formation of wrinkles on the skin.

In addition, collagen is a matrix protein that plays an important role in retaining moisture in the dermal layer. These matrix proteins absorb moisture and increase the water retention capacity within the structure they form, allowing the skin to maintain an appropriate level of moisture. It is known to be involved in maintaining skin elasticity.

Currently, retinoids, adenosine, animal placenta-derived proteins, and chlorella extract are known as cosmetics for improving skin elasticity and improving wrinkles. Retinol, the most well-known substance, is a substance that promotes collagen synthesis, but it is unstable, and its usage is limited due to safety issues such as irritation and redness when applied to the skin. Chlorella extract, etc., has minimal effects and does not actually improve skin elasticity and improve wrinkles. It is known to be difficult to anticipate.

The human body has a balance of pro-oxidation substances and antioxidant substances, but when this balance is lost due to various factors and the trend goes in the direction of promoting oxidation, oxidative stress is induced in the body, causing cell damage and damage. It causes pathological diseases. Reactive oxygen species (ROS), which are the direct cause of this oxidative stress, are chemically unstable and highly reactive, so they can easily react with various biomaterials such as DNA, proteins, lipids, and carbohydrates, and can destroy macromolecules in vivo. It attacks cells and tissues, causing irreversible damage or causing mutations, cytotoxicity, and cancer.

Meanwhile, within the human body, macrophages react to pathogens and induce inflammation such as tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Generates nitric oxide (NO) and prostaglandin (prostaglandin E2, PGE2) by synthesizing inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ) is created. Physiologically, NO plays a variety of roles, including eliminating bacteria and tumors, regulating blood pressure, and mediating nerve transmission. However, when an inflammatory response occurs, the expression of iNOS increases in related cells, producing a large amount of NO. Excessively produced NO causes tissue damage, genetic mutation, nerve damage, etc., and increases vascular permeability, leading to edema. Promotes inflammatory reactions such as:

During an inflammatory reaction, various free radicals are generated along with various inflammation-inducing factors. Normal free radicals are involved in maintaining cellular homeostasis and affect cell differentiation, growth, survival, and aging. Among these free radicals, reactive oxygen species (ROS) are constantly generated in mitochondria within cells through the oxidation and reduction processes of oxygen caused by respiration and immune reactions. Harmful ROS generally include superoxide dismutase, catalase, glutathione peroxidase, glutamine reductase, vitamin C, vitamin E, uric acid, bilirubin and It is eliminated through the action of the same antioxidant system. However, when the balance between generation and elimination of free radicals is broken, oxidative stress occurs, causing various pathological changes such as inflammation, aging, or cancer. In fact, it has been confirmed that oxidative stress is increased in many clinical diseases, and various studies are being conducted on new antioxidant substances to reduce oxidative stress.

Research is continuing on ingredients that protect the skin from various causes such as environmental irritation, stress, and chemical treatments, and are eco-friendly and harmless to the environment and humans.

As described above, the above-described cosmetic compositions have problems such as causing economic burden and pain to people or causing side effects, and having temporary effects or using only fragmentary pharmacological mechanisms, so the effects are more excellent and lasting, The demand for compositions without side effects is increasing.

Against this background, the present inventors conducted research on substances with excellent skin whitening, skin elasticity enhancement, wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects, and developed a composition containing mixed extracts of rose, peony and iris as active ingredients. By discovering that it reduces the total amount of melanin, promotes collagen synthesis, moisturizes, has anti-inflammatory and antioxidant effects, it has led to the completion of cosmetic compositions, functional food compositions and quasi-drug compositions using it.

Republic of Korea Patent No. 10-0993603 Republic of Korea Patent No. 10-1638538

The main object of the present invention is to provide a cosmetic composition containing mixed extracts of rose, peony and iris as active ingredients.

Another object of the present invention is to provide a functional food composition containing mixed extracts of rose, peony and iris as active ingredients.

Another object of the present invention is to provide a quasi-drug composition containing a mixed extract of rose, peony and iris as an active ingredient.

In order to achieve the above object, one aspect of the present invention provides a cosmetic composition containing mixed extracts of rose, peony, and iris as an active ingredient.

The term "rose (Rosa hybrida)" used in the present invention is a temperate evergreen shrub that loves sunlight. At the end of the 18th century, Asian original species were introduced to Europe, and crossbreeding between these European and Asian original species occurred, creating varieties with many ecological changes, such as flower color and shape, as well as four-season and flowering characteristics. In general, it is white, red, yellow, or pink, but its shape, shape, and color vary greatly depending on the variety. The timing and duration of flower blooming also varies greatly depending on the variety. Roses improve blood circulation and are known to be effective in diuresis, detoxification, menstrual irregularities, menstrual pain, constipation, and gastroenteritis.

The term "paeonia lactiflora" used in the present invention is a perennial dicotyledonous plant belonging to the peony family and grows in mountainous areas. The flowers are beautiful and are used for gardening. In China, it was already cultivated for ornamental purposes during the Jin and Myeongseong Dynasties, and its cultivation history has a long history. The root is said to be effective in pain relief, abdominal pain, menstrual pain, amenorrhea, hematemesis, anemia, tarmaca, etc. and is widely used as a medicinal herb in oriental medicine.

The term "Iris" used in the present invention is a plant of the Iris family of the monocot family Liliaceae, and about 200 species grow in temperate regions of the Northern Hemisphere, and 14 species exist in Korea. There are many varieties, but they are divided into four lines: English iris, German iris, Spanish iris, and Dutch iris. The rhizome is used as a medicine and is known to be effective for indigestion, bruises, and hemorrhoids.

In the present invention, “extract” refers to a liquid component obtained by immersing the desired substance in various solvents and then extracting the substance for a certain period of time at room temperature, low temperature, or heated state, and removing the solvent from the liquid component. It refers to the result of solid content, etc. In addition, in addition to the above results, it can be comprehensively interpreted to include all dilutions of the results, concentrates thereof, crude preparations, purifications, etc. of the above results.

In the present invention, the extract refers to rose, peony, and iris extract. The rose, peony, and iris extracts can be extracted from various organs of natural, hybrid, and varietal plants, for example, roots, aerial parts, stems, leaves, flowers, fruit bodies, and fruit peels, as well as plant tissues. do. The rose, peony, and iris extracts can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited as long as it can obtain an extract, but may specifically be water, a polar solvent, or a non-polar solvent, and more specifically, water, a lower alcohol having 1 to 6 carbon atoms ( methanol, ethanol, propanol, or butanol, etc.), or a mixed solvent thereof, and most specifically, it may be methanol or a mixed solvent thereof. Additionally, in the present invention, sugars may be included as a solvent for the extract. Specifically, sugars such as honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide may be used, but are not limited thereto.

In addition, the method for obtaining the extract is not particularly limited as long as it is possible to obtain the rose, peony, and iris extract, but specifically, the roots, stems, leaves, fruits, and flowers of the rose, peony, and iris. Methods such as cold needle extraction, heat extraction, extraction by applying ultrasonic waves, and reflux extraction using a reflux condenser can be used by immersing dried materials, processed products, etc. in the solvent and extracting at room temperature.

The term "mixed extract" used in the present invention may be an extract obtained by mixing the extracts of each component of rose, peony, and iris in an appropriate weight mixing ratio, and may also be an extract obtained by mixing rose, peony, and iris in an appropriate weight mixing ratio. It could be something. The mixed extract was confirmed to be more effective in skin whitening, improving skin elasticity or wrinkle improvement, skin moisturizing, preventing skin aging, antioxidant, and improving skin inflammation than the individual rose, peony, and iris single extracts. It was found to have an excellent synergistic effect. You can.

The weight mixing ratio of rose extract, peony extract, and iris extract is a weight ratio (w/w) of 1~25:1~25:1~25, specifically, a weight ratio (w/w) of 1~15:1~15:1~15. w), more specifically, it may be a weight ratio (w/w) of 1 to 5: 1 to 5: 1 to 5, and may have the same weight mixing ratio.

In the present invention, sugars may be additionally included in the mixed extract. Sugars include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide, and any substance commonly used in the art can be used. More specifically, honey and royal jelly can be used as sugars.

The term “honey” used in the present invention refers to what bees collect from the nectar glands of flowers and store it as food. Honey is divided into native honey and beekeeping honey depending on the type of bee, and is called acacia honey, bush honey, rape honey, chestnut honey, buckwheat honey, etc. depending on the flower. The color and taste vary depending on the type of flower. Honey is about 80% carbohydrates, with 36-38% fructose, 34-36% glucose, and 2-3% sugar and dextrin. In addition, it contains 0.2-0.3% protein, 0.05-0.2% ash, vitamin B complex B1, B2, B6, pantothenic acid, formic acid, lactic acid, malic acid, pigment, gum, wax, enzyme, etc. In Korea, honey is widely used for edible and medicinal purposes, and is also used as a sweetener.

The term "royal jelly" used in the present invention refers to a 'gift offered to the king' by worker bees and food for the queen bee. Royal jelly is known to have various effects as it contains a mysterious nutritional substance containing proteins, fats, carbohydrates, and various trace elements, as well as an ingredient called R, which has not yet been discovered. In addition, royal jelly itself is known to be nutritious and effective for skin care.

According to one embodiment of the present invention, when sugars such as honey and royal jelly are added to the mixed extract of rose, peony, and iris, collagen synthesis is enhanced and melanin is increased compared to single extracts of rose, peony, and iris. It was confirmed that production was suppressed, expression of HAS, a moisturizing factor, was increased, radical scavenging ability, and NO production were suppressed.

Through this, when sugars such as honey and/or royal jelly are added to the mixed extract of rose, peony, and iris, it creates a synergistic effect with the extract, resulting in better skin whitening, skin elasticity, skin moisturizing, anti-inflammatory, and antioxidant effects. And it was found.

In the present invention, the cosmetic composition may have effects such as skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects, but is not limited thereto.

“Skin whitening” in the present invention refers to not only brightening skin tone by inhibiting the synthesis of melanin pigment, but also improving hyperpigmentation of the skin such as spots or freckles caused by ultraviolet rays, hormones, or genetics.

According to one specific embodiment, the total amount of melanin is measured after treating melanoma cells with a mixed extract of rose, peony, and iris of the present invention or a composition obtained by adding honey and/or royal jelly to a mixed extract of rose, peony, and iris. As a result, it was confirmed that the mixed extract can be used for skin whitening because it shows a better melanin production inhibition effect on melanoma cells compared to the control group or single extract (Table 3).

Through this, when honey and/or royal jelly are added to a mixed extract of rose, peony, and iris, or a mixed extract of rose, peony, and iris, it produces a synergistic effect compared to a single extract, resulting in a better skin whitening effect. Could know.

In the present invention, "promoting skin elasticity" refers to alleviating the degree of sagging or sagging skin, in which elastic fibers made of elastin exist together with collagen fibers called collagen, a state in which sufficient elastin and collagen are present. This means that skin elasticity is maintained. In the present invention, “wrinkle improvement” refers to suppressing or inhibiting the formation of wrinkles on the skin or alleviating wrinkles that have already formed.

According to one specific example, the synthesis of procollagen was measured by culturing HF cells treated with a mixed extract of rose, peony, and iris of the present invention or a composition obtained by adding honey or royal jelly to a mixed extract of rose, peony, and iris. As a result, it was confirmed that the collagen synthesis effect was the best in the mixed extract (Table 2).

Through this, when honey and/or royal jelly are added to a mixed extract of rose, peony, and iris, or a mixed extract of rose, peony, and iris, it creates a synergistic effect compared to a single extract, and has a better effect in improving skin elasticity. And it was found.

“Skin moisturizing” in the present invention means increasing moisture in the skin and maintaining a moist state. The skin moisturizing effect can help improve skin wrinkles and increase elasticity.

According to one specific embodiment, keratinocytes (HaCaT, human keratinocytes) are cultured in a mixed extract of rose, peony, and iris of the present invention, or a composition obtained by additionally treating the mixed extract of rose, peony, and iris with honey or royal jelly. As a result of a moisturizing experiment comparing the amount of HAS3 mRNA, it was confirmed that the mixed extract increased HAS3 mRNA expression compared to the control and single extract (Table 4).

According to one specific embodiment, keratinocytes (HaCaT, human keratinocytes) are cultured in a composition obtained by adding honey and/or royal jelly to a mixed extract of rose, peony, and iris or a mixed extract of rose, peony, and iris of the present invention, , As a result of testing the moisturizing power by comparing the amount of HAS3 mRNA, it was confirmed that HAS3 mRNA expression was increased when treated with the mixed extract compared to the control group and single extract (Table 4).

These results showed that the mixed extract can have a skin moisturizing effect.

In addition, when honey and/or royal jelly are added to a mixed extract of rose, peony, and iris or a mixed extract of rose, peony, and iris, it produces a synergistic effect compared to a single extract, resulting in a better effect on skin moisturization. And it was found.

In the present invention, "antioxidation" refers to the action of suppressing oxidation. The human body has a balance of prooxidants and antioxidants, but this balance can become unbalanced due to various factors. When this is achieved and the tendency is toward promoting oxidation, oxidative stress is induced, causing potential cell damage and pathological diseases. Reactive oxygen species (ROS), which are the direct cause of this oxidative stress, are unstable and highly reactive, easily reacting with various biomaterials, and attacking macromolecules in the body, causing irreversible damage to cells and tissues, mutations, and mutations. It causes cytotoxicity and carcinogenesis. Reactive nitrogen species (RNS), such as NO, HNO ₂ , and ONOO ^- , are produced in large quantities due to the immune response of macrophages, neutrophils, and other immune cells during inflammatory reactions, and ROS is also produced at this time. The above-described free radicals oxidize and destroy cells in the body, thereby exposing them to various diseases. Therefore, if the mixed extract of the present invention is included in cosmetics, it can contribute to health promotion by achieving an antioxidant effect.

According to one specific example, the DPPH radical scavenging ability (Table 5) of the mixed extract of rose, peony, and iris of the present invention was analyzed and it was confirmed to have strong antioxidant ability. It was confirmed that the IC ₅₀ value of the mixed extract was reduced compared to the single extract. These results showed that the mixed extract can exhibit antioxidant effects.

Through this, it can be seen that when honey and/or royal jelly are added to a mixed extract of rose, peony, and iris or to a mixed extract of rose, peony, and iris, it produces a synergistic effect compared to a single extract and has a superior antioxidant effect. I was able to.

“Anti-inflammatory” in the present invention refers to the action of suppressing inflammation, and the control of inflammatory response is known to be very complex, and is known to enhance the in vivo repair system and reduce damage. However, if the inflammatory response continues due to repeated tissue damage or regeneration, ROS and RNS are excessively produced in inflammation-related cells, resulting in permanent genetic modification. As such, ROS and RNS are deeply related to the inflammatory response that regulates the actions of various cells in vivo. During the inflammatory process, large amounts of proinflammatory cytokines, nitric oxide (NO), and prostaglandin E2 (PGE2) are released into inducible nitric oxide synthase (iNOS) and cyclooxygenase. It is produced by (cyclooxygenase-2, COX-2). Inflammation is a cause of various inflammatory diseases, and the composition containing the mixed extract of rose, peony and iris of the present invention can have a preventive and ameliorating effect on various inflammatory diseases through anti-inflammatory action.

According to one specific example, as a result of analyzing the NO production inhibition effect of the mixed extract of rose, peony, and iris of the present invention, it was confirmed that the mixed extract had a higher NO production inhibition ability when treated at the same concentration compared to the single extract, It was confirmed that the mixed extract had a strong anti-inflammatory effect (Table 6).

Through this, when honey and/or royal jelly are added to a mixed extract of rose, peony, and iris or a mixed extract of rose, peony, and iris, it produces a synergistic effect compared to a single extract and has a superior anti-inflammatory effect. Could know.

The cosmetic composition according to the present invention includes solution, external ointment, cream, foam, nourishing lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen cream, sun oil, suspension, Can be prepared in a formulation selected from the group consisting of emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. That is not the case.

In addition, the cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers that are blended with general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, moisturizer, lower alcohol, Thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately mixed, but are not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, the carrier ingredient may be animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as the carrier ingredient, and especially in the case of a spray, chloro May contain propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. there is.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and miso. Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier ingredients. You can.

The cosmetic composition of the present invention not only contains sugars of honey or royal jelly in addition to the mixed extract of rose, peony, and iris or the mixed extract of rose, peony, and iris, but also contains auxiliaries commonly used in cosmetic compositions such as hydrophilic or It may contain lipophilic gelling agents, hydrophilic or lipophilic activators, preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, odorants and dyes.

As another aspect for achieving the above object, the present invention provides a functional food composition containing mixed extracts of rose, peony, and iris as an active ingredient.

In the present invention, the functional food composition may additionally include sugars. Sugars include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide, and any substance commonly used in the art can be used.

In the present invention, the functional food composition may have effects such as skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects, but is not limited thereto.

The rose, peony, iris, extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory are as described above. The functional food composition may be used in the form of a health functional food, but is not limited thereto.

The functional food composition containing the mixed extract of rose and iris included in the functional food composition of the present invention as an active ingredient may be included in the form of an extract thereof, a fraction thereof, or a processed product thereof. Additionally, the composition may include food additives that are foodologically acceptable in addition to the active ingredients.

In the present invention, “food supplement” refers to a component that can be added to food as an auxiliary ingredient, and can be appropriately selected and used by a person skilled in the art as it is added to manufacture each type of health functional food. Examples of food supplements include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, and protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc., but the types of food supplements of the present invention are not limited to the above examples.

The functional food composition of the present invention may include health functional foods. In the present invention, “health functional food” refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills using raw materials or ingredients with functional properties useful to the human body. Here, “functionality” means controlling nutrients for the structure and function of the human body or obtaining useful effects for health purposes, such as physiological effects. The health functional food of the present invention can be manufactured by a method commonly used in the industry, and can be manufactured by adding raw materials and components commonly added in the industry. Additionally, the formulation of the health functional food can also be manufactured without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be manufactured in various types of formulations, and unlike general drugs, it has the advantage of being made from food as a raw material and has no side effects that may occur when taking the drug for a long period of time, and is excellent in portability, making it suitable for use in the formulation of the present invention. Health functional foods can be taken as supplements to whiten skin, improve skin elasticity or improve wrinkles, or to improve skin moisturization, antioxidant, and anti-inflammatory effects.

There is no limit to the form that the health functional food of the present invention can take, and it can include all foods in the conventional sense and can be used interchangeably with terms known in the art, such as functional food. In addition, the health functional food of the present invention can be manufactured by mixing known additives with other appropriate auxiliary ingredients that can be included in the food according to the selection of a person skilled in the art. Examples of foods that can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes, etc., and they can be prepared by adding the compound represented by Chemical Formula 1 according to the present invention to juices, teas, jellies, etc. prepared as main ingredients. It also includes foods used as feed for animals.

As another aspect for achieving the above object, the present invention provides a quasi-drug composition containing mixed extracts of rose, peony, and iris as an active ingredient.

In the present invention, the quasi-drug composition may contain sugars. Sugars include, but are not limited to, honey, royal jelly, sugar, fructose, sugar alcohols, and fructooligosaccharide, and any substance commonly used in the art can be used.

In the present invention, the quasi-drug composition may have effects such as skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory effects, but is not limited thereto.

The rose, peony, iris, extract, mixed extract, honey, royal jelly, skin whitening, skin elasticity improvement and wrinkle improvement, skin moisturizing, antioxidant, and anti-inflammatory are as described above.

In addition to the above ingredients, the quasi-drug composition of the present invention may further include pharmaceutically acceptable carriers, excipients, or diluents as needed. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effect of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It can be included.

Representative examples of pharmaceutically acceptable carriers, excipients or diluents of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium. Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil. , injectable esters, Wethepsol, Macrogol, Tween 61, Kakaoji, Lauridge, etc.

In addition, when the composition containing the mixed extract of rose, peony and iris of the present invention as an active ingredient is used as a quasi-drug, it may additionally contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients. If additional skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing ingredients are included, the skin whitening, elasticity enhancement, wrinkle improvement, and moisturizing effects of the composition of the present invention can be further increased. When adding the above ingredients, skin safety due to combined use, ease of formulation, and stability of the active ingredients can be taken into consideration. The quasi-drug composition includes whitening ingredients known in the art, such as substances that inhibit tyrosinase enzyme activity such as Kojic acid and Arbutin, Hydroquinone, Vitamin-C (L-Ascorbic acid); Skin elasticity, wrinkle improvement or moisturizing ingredients known in the art include retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract; and one or two or more ingredients selected from the group consisting of their derivatives and various plant extracts. Additional ingredients may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety and ease of use.

The quasi-drug composition of the present invention may include, but is not limited to, disinfectant cleaner, shower foam, ointment, wet tissue, coating agent, etc., and the formulation method, dosage, usage method, and components of the quasi-drug are commonly known in the technical field. It can be appropriately selected from the techniques.

In addition, the quasi-drug composition containing the mixed extract of rose, peony and iris of the present invention as an active ingredient is used in a method for whitening skin, improving skin elasticity or improving wrinkles, or moisturizing skin, including the step of applying it to the skin of an individual. can be used for The subject includes mammals, including rats, livestock, humans, etc., without limitation.

The cosmetic composition containing the mixed extract of rose, peony and iris of the present invention as an active ingredient has excellent skin whitening, elasticity improvement or wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory effects. Specifically, the composition containing the mixed extract of the present invention shows the effect of reducing the total amount of melanin, has an excellent effect of promoting collagen synthesis and moisturizing effect, and has an inhibitory effect on NO production and a DPPH radical scavenging effect compared to a single extract, so it is used for skin whitening and elasticity improvement. It has high utility as a cosmetic composition for wrinkle improvement, skin moisturizing, antioxidant and anti-inflammatory purposes. Additionally, the composition containing the mixed extract of rose, peony and iris of the present invention as an active ingredient can be used as a functional food composition and quasi-drug composition.

Hereinafter, the present invention will be explained in detail by the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Manufacturing example 1. Preparation of rose, peony, and iris extracts

After washing the roses, peonies, and iris with water, the washed roses, peonies, and iris were placed in an extraction vessel and extracted slowly at room temperature by adding an extraction solvent. The obtained extract was filtered to remove solids using filter paper, etc., and then the extract was concentrated under reduced pressure to prepare each extract.

Manufacturing example 2. Preparation of mixed extract

A mixed extract was prepared by injecting equal amounts of the single extracts of rose, peony, and iris prepared in Preparation Example 1. Mineral water or sugars such as honey and/or royal jelly were added to each mixed extract. An example of the preparation ratio of the mixed extract is shown in Table 1.

ingredient Example 1 ( % ) Example 2 ( % ) Example 3 ( % ) Example 4 ( % ) mineral water 25 - - - rose 25 25 25 25 peony 25 25 25 25 Iris 25 25 25 25 honey - 25 - 12.5 royal jelly - - 25 12.5

The contents of Examples 1 to 4 were determined based on each extract of Preparation Example 1. In Examples 1 to 4, the maximum content ratio was adjusted so that each extract was mixed in equal proportions to produce a synergistic effect through collaborative action.

Experiment example 1. Collagen synthesis enhancement effect

Collagen synthesis enhancement effect using cells cultured as follows for the single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and mixed extracts of Examples 1 to 4 prepared in Preparation Example 2. was measured.

The synthesis of procollagen was measured using a kit (procollagen type-1 C-peptide EIA kit, Takara, Japan). HF (Human Fibroblast) cells were placed in a ^24- well plate at a density of 5 0.01% by weight of the mixed extract of Examples 1 to 4 was added and cultured in an incubator at 37°C and 5% CO ₂ for 48 hours. Add 100 ㎕ of antibody-POD conjugate sol'n to an anti-PIP antibody-coated 96 well plate and add each single solution prepared in Preparation Example 1 without any additives. 20 ㎕ of the standard solution was added to the extract, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, blocked from light with aluminum foil, and incubated at 37°C for 3 hours.

The culture medium was removed, washed four times with PBS, thoroughly removed, 100 ㎕ of substrate solution was added, left at room temperature for 15 minutes, 100 ㎕ of H ₂ SO ₄ was added, shaken gently, and absorbance was measured at 450 nm. The experimental results are shown in Table 2. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Collagen expression Increase (%) (n=3) division Collagen synthesis increase rate (%) Untreated 0 rose 1.4 peony 1.9 Iris 2.7 honey 7.05 royal jelly 11.73 Example 1 8.06 Example 2 18.5 Example 3 22.42 Example 4 25.835

As a result, as shown in Table 2, it was confirmed that the treatment of the mixed extract of Example 1 prepared in Preparation Example 2 had a better effect of increasing collagen synthesis than each single extract prepared in Preparation Example 1, and Preparation Example 2 It was confirmed that the mixed extracts of Examples 2 to 4 prepared in were more effective in increasing collagen synthesis than the mixed extract of Example 1 by honey and/or royal jelly.

Experiment example 2. Melanin production inhibition effect

In order to confirm the whitening effect of the single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, cells were cultured as follows. The melanin suppression effect was measured using .

Specifically, the whitening efficacy of the sample was evaluated by measuring the degree to which the amount of melanin pigment present in melanocytes was reduced. The sample was diluted to 0.01% by weight, treated with B16 melanocytes, cultured for 48 hours, and melanin was quantified to evaluate whitening efficacy. The melanin content was calculated based on the total protein amount and then calculated as a percentage based on the DMSO control group. Arbutin 100 ppm (Lim YJ et al., 2009, Arch Pharm Res.) was used as a positive control.

The results are shown in Table 3 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

amount of melanin produced (%) (n=3) sample Melanin production amount (%) Untreated 100 Arbutin (positive control) 55 rose 82 peony 86 Iris 115 honey 120 royal jelly 146 Example 1 79 Example 2 65 Example 3 73 Example 4 51

As a result, as shown in Table 3, it was confirmed that the treatment of the mixed extract of Example 1 prepared in Preparation Example 2 had a superior melanin production inhibition effect than each single extract prepared in Preparation Example 1, and Preparation Example 2 It was confirmed that the mixed extract of Examples 2 to 4 prepared in has a more excellent melanin production inhibition effect than the mixed extract of Example 1 by honey and/or royal jelly.

Experiment example 3. Moisturizing factor synthesis factor enhancement effect

In order to confirm the effect of enhancing moisturizing factor synthesis elements for the single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, the following The skin moisturizing effect was measured using cells cultured together.

Specifically, under conditions of 37°C and 5% CO ₂ , 10% FBS (Gibco), streptomycin 100 ㎍/㎖, penicillin 100 ㎍/㎖, amphotericin B 0.25 ㎍/㎖. Keratinocytes (HaCaT, human keratinocytes) were proliferated using DMEM medium supplemented with ㎖.

The formed keratinocytes ( _HaCaT , human keratinocytes) were dispensed into 6 wells at a density of ⁵ The cultured medium was washed with PBS to remove FBS. To the medium from which FBS was removed, untreated, single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and Examples 1, 2, 3, and 4 prepared in Preparation Example 2 were added to 0.01% by weight. After treating each with 3 ml, they were cultured again, and the amount of HAS3 (Hyaluronan Synthases 3) mRNA was compared. RNA was synthesized using an RNA extraction kit (RNeasy Mini Kit, Qiagen, USA), cDNA was synthesized using SuperScript II reverse transcriptase (Invitrogen, USA), and Platinum PCR supermix. (Invitrogen, USA)) and PCR was performed using the prepared primers. For cDNA synthesis, 1 μg of total RNA was heated at 50°C for 1 hour and 85°C for 5 minutes, and then the reaction was stopped. PCR amplification consisted of denaturation at 94°C for 50 seconds, annealing at 60°C for 50 seconds, and extension at 72°C for 50 seconds. This was repeated for 26 cycles, and the final extension was performed at 72°C for 5 minutes, and the amplified product was electrophoresed on a 2% agarose gel to determine gene expression using the Fusion Fx5 detection system (VilberLourmat, Germany). It was confirmed.

The results are shown in Table 4 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Increased HAS expression (%) (n=3) division HAS mRNA manifestation ( % ) Untreated 100 rose 103 peony 93 Iris 95 honey 112 royal jelly 115 Example 1 108 Example 2 120 Example 3 128 Example 4 140

As a result, as shown in Table 4, it was confirmed that when processing the mixed extract of Example 1 prepared in Preparation Example 2, HAS expression increased compared to each single extract prepared in Preparation Example 1. It was confirmed that the mixed extract of Examples 2 to 4 increased HAS expression more than the mixed extract of Example 1 by honey and/or royal jelly.

Experiment example 4. Antioxidant effect

In order to confirm the antioxidant effect of the single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, cells were cultured as follows. The skin antioxidant effect was measured using .

Specifically, the radical scavenging ability of the sample was evaluated using the change of DPPH from purple to yellow when the radicals present in DPPH are scavenged. Vit C was used as a control. The sample was used after dissolving it in methanol. The experimental method was to mix 100 μl of each sample with a 0.15 mM DPPH solution and react at room temperature for 30 minutes, and then measure the change in color of DPPH by measuring the absorbance (A540) at 540 nm. The IC ₅₀ value was obtained from the A540 value to compare and evaluate the radical scavenging ability.

The results are shown in Table 5 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

radical scavenging ability (IC ₅₀ ) (n=3) division IC ₅₀ Vit C 0.0005 rose 0.0242 peony 0.0231 Iris 0.27 honey 12.04 royal jelly 6.049 Example 1 0.021 Example 2 0.0224 Example 3 0.0198 Example 4 0.01899

As a result, as shown in Table 5, it was confirmed that the mixed extract of Example 1 prepared in Preparation Example 2 had a superior antioxidant effect than each single extract prepared in Preparation Example 1. It was confirmed that the mixed extract of Examples 2 to 4 had a better antioxidant effect than the mixed extract of Example 1 due to honey and/or royal jelly.

Experimental Example 5. Anti-inflammatory effect

In order to confirm the anti-inflammatory effect of the single extracts of rose, peony, and iris prepared in Preparation Example 1, honey, royal jelly, and the mixed extract of Examples 1 to 4 prepared in Preparation Example 2, cells were cultured as follows. The skin anti-inflammatory effect was measured using .

Specifically, the NO production inhibition effect of the extracts was measured using Raw 264.7 cells. As a positive control, 20 mg/ml L-NMMA1 (NOS inhibitor) was used, and the sample was diluted to a concentration of 0.01% by weight, treated 30 minutes ago, treated with 1 μg/ml LPS, and cultured for 24 hours using the NO Quantification Kit. The ability to inhibit NO production was evaluated as follows.

The results are shown in Table 6 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

NO production inhibition effect (%) (n=3) division NO production inhibition effect ( % ) Positive control group (NOS inhibitor) 42 rose 20 peony 18 Iris 16 honey 13.1 royal jelly 15.5 Example 1 26 Example 2 33 Example 3 37 Example 4 45

As a result, as shown in Table 6 above, when processing the mixed extract of Example 1 prepared in Preparation Example 2, it was confirmed that the NO production inhibition ability was higher than that of each single extract prepared in Preparation Example 1, and the The mixed extracts of Examples 2 to 4 were confirmed to have a higher NO production inhibition ability than the mixed extract of Example 1 due to honey and/or royal jelly.

That is, it can be seen that the mixed extract according to the present invention (Example 1) has a superior synergistic effect than the individual rose, peony, and iris single extracts, and through Examples 2 to 4, the mixed extract (Example 1) with honey and/or royal jelly added In this case, it can be seen that the synergistic effect of the mixed extract is higher.

These results show that the mixed extract is very effective in skin whitening, improving skin elasticity or wrinkles, moisturizing skin, preventing skin aging, antioxidant, and improving skin inflammation.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.

Claims (13)

delete delete A cosmetic composition for skin whitening containing mixed extracts of rose, peony and iris as active ingredients.
A cosmetic composition for improving skin elasticity or improving wrinkles, comprising mixed extracts of rose, peony and iris as active ingredients.
A cosmetic composition for moisturizing skin containing mixed extracts of rose, peony and iris as active ingredients.
An antioxidant cosmetic composition containing mixed extracts of rose, peony and iris as active ingredients.
An anti-inflammatory cosmetic composition containing mixed extracts of rose, peony and iris as active ingredients.
A functional food composition containing mixed extracts of rose, peony and iris as active ingredients,
The functional food composition is for skin whitening, improving skin elasticity or wrinkles, moisturizing the skin, antioxidant or anti-inflammatory.
According to clause 8,
A functional food composition further comprising honey, royal jelly, or both.
delete A quasi-drug composition containing mixed extracts of rose, peony and iris as active ingredients,
The quasi-drug composition is for skin whitening, improving skin elasticity or wrinkles, moisturizing the skin, antioxidant, or anti-inflammatory.

According to clause 11,
A quasi-drug composition further comprising honey, royal jelly, or both.
According to any one of claims 3 to 7,
A cosmetic composition further comprising honey, royal jelly, or both.