KR102534239B1 - Cosmetic composition comprising glutathione, Maqui Berry extract and Mulberry root extract - Google Patents

Abstract

The present invention relates to a cosmetic composition, a food composition, an inner beauty composition, and a quasi-drug composition containing glutathione, a maqui berry extract, and a moth extract as active ingredients. The composition containing glutathione, maqui berry extract, and moth berry extract of the present invention as active ingredients has excellent skin whitening, skin trouble improvement, skin moisturizing or skin wrinkle improvement effects, so it can be used for skin whitening, skin trouble improvement, skin moisturizing or skin It is highly applicable as a cosmetic for wrinkle improvement. In addition, the composition of the present invention can be used as a quasi-drug composition, a food composition, or an inner beauty composition.

Description

COSMETIC COMPOSITION COMPRISING ACTIVE INGREDIENTS OF GLUTATHIONE, MAQUI Berry EXTRACT, AND COSMETIC Berry EXTRACT

The present invention relates to a cosmetic composition, a quasi-drug composition, a food composition, and an inner beauty composition containing glutathione, a maqui berry extract, and a moth bark extract as active ingredients. Specifically, the present invention relates to a composition for skin whitening, skin trouble improvement, skin moisturization, and skin wrinkle improvement comprising glutathione, maqui berry extract, and moth extract as active ingredients.

There are many factors that determine human skin color, and the activity of melanocytes that produce melanin pigment, the distribution of blood vessels, the thickness of skin, and other genetic factors play an important role. Among them, the activity of melanocytes in the human body to produce melanin pigment by the action of various enzymes, such as tyrosinase, is known to be the most important factor.

On the other hand, melanin is a dark brown pigment produced by oxidative polymerization of phenolic compounds. It not only determines human skin color, but also absorbs ultraviolet rays or exhibits an antioxidant effect as a free radical scavenger to protect the skin. is playing an important role. However, if the skin is excessively exposed to UV rays, or if melanin is excessively produced due to factors such as hormonal changes or stress, it can cause pigmentation in the skin, making the skin dark and cloudy, or causing spots or freckles, which can negatively affect skin beauty. can

Commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C or derivatives thereof, and various plant extracts. They can brighten the skin tone and achieve skin whitening by inhibiting the synthesis of melanin pigment, or improve hyperpigmentation of the skin, such as spots and freckles caused by ultraviolet rays, hormones, or genetic factors. However, there is a problem in that the amount of use is limited due to safety problems such as irritation and redness when applied to the skin, or a practical effect cannot be expected because the effect is insignificant.

Accordingly, the inventors of the present invention have made intensive efforts to develop substances with high utilization value as cosmetics or quasi-drug compositions for skin whitening without side effects and toxicity to the human body using natural raw materials, and as a result, glutathione and maqui The present invention was completed by finding that a composition containing a berry extract as an active ingredient has excellent effects not only on skin whitening but also on skin trouble improvement, moisturizing and wrinkle improvement.

One object of the present invention is to provide a cosmetic composition for skin whitening, skin trouble improvement, skin moisturization, and skin wrinkle improvement, containing glutathione, maqui berry extract, and moth bark extract as active ingredients.

Another object of the present invention is to provide a quasi-drug composition for skin whitening, skin trouble improvement, skin moisturization, and skin wrinkle improvement, containing glutathione, maqui berry extract, and moth bark extract as active ingredients.

A detailed description of this is as follows. Meanwhile, each description and embodiment disclosed in this application may also be applied to each other description and embodiment. That is, all combinations of various elements disclosed in this application fall within the scope of this application. In addition, the scope of the present application is not to be construed as being limited by the specific descriptions described below.

One aspect of the present invention for achieving the above object provides a cosmetic composition comprising glutathione, maqui berry extract, and moth extract as active ingredients.

The composition of the present invention is excellent in skin whitening, skin trouble improvement, skin moisturizing, or skin wrinkle improvement efficacy, so it is highly applicable as a cosmetic, and in particular, it can exhibit a remarkably excellent effect as a cosmetic for skin whitening.

In the present invention, the term "morus albaica (桑白皮)" refers to a medicine made from the root bark of a mulberry tree (Morus alba L.) or a plant of the same genus, and is effective in reducing asthma and blood pressure in oriental medicine, and has a diuretic action It is known that there are

In the present invention, the term "Maqui berry ( Aristotelia chilensis )" refers to an evergreen broad-leaved tree of the order Oxalinaceae, or its fruit, native to the rainforest region of southern Chile, and is rich in anthocyanins and polyphenols, which are antioxidants. Traditionally, it is known to be effective in arthritis, hyperlipidemia, diabetes and heart disease. In the present invention, maqui berry can be purchased commercially, or harvested or cultivated in nature, without limitation, and can be used without limitation in parts such as flowers, leaves, fruits, seeds, roots or stems.

In the present invention, the term "extract" refers to an extract obtained by extraction of the above-mentioned Mortice bark, Maqui berry, etc., a diluted or concentrated solution of the above-mentioned extract, a dried product obtained by drying the above-mentioned extract, a crude or purified product of the above-mentioned extract, or a mixture thereof. Etc., the extract itself and extracts of all formulations that can be formed using the extract. The extract of the present invention can be extracted from natural, hybrid, and mutant plants without limitation, and can also be extracted from plant tissue culture.

In the present invention, a method for extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

In the present invention, the type of extraction solvent used to extract maqui berry, moth berry, etc. is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more.

In the present invention, water or ethanol may be preferably used as a solvent for extracting moth bark, maqui berry, and the like. A solvent extract can be prepared by extracting Mortalis, Maqui berry, etc. at least once using the solvent, and a dried extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying can be prepared.

In the present invention, the extract obtained by hot water extraction or cold brewing is filtered to remove floating solid particles by filtering out the particles using, for example, nylon or by using cryofiltration, and then used as it is or freeze-dried, hot-air-dried, It can be dried using spray drying or the like.

In the present invention, the term "fraction" means a result obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components. In the present invention, the fractionation method for obtaining the fraction is not particularly limited, and may be performed according to a method commonly used in the art. A non-limiting example of the fractionation method is a method of obtaining a fraction from the extract by treating the extract obtained by extracting Mortice bark, Maqui berry, etc. with a predetermined solvent.

In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohol is used among the fractionation solvents, C1 to C4 lower alcohols may be specifically used.

In the present invention, the term "glutathione" is a water-soluble, crystalline peptide compound having a chemical formula of C ₁₀ H ₁₇ N ₃ O ₆ S and a molecular weight of about 307.3235 g/mol, which includes glutamate, cysteine and It is composed of three peptides, glycine, and is known to play an important role in redox reactions in vivo.

The glutathione is an antioxidant present in vivo and can effectively remove radicals in vivo by reacting reversibly with antioxidants in vivo, such as ascorbic acid (vitamin C) and tocopherol (vitamin E).

The glutathione can play a very important role as the final radical acceptor in the biological system related to the radical elimination.

Another aspect of the present invention provides a cosmetic composition for skin whitening comprising glutathione, a maqui berry extract, and a moth extract as active ingredients.

In the present invention, the term "skin whitening" means not only brightening the skin tone by inhibiting the synthesis of melanin pigment, but also improving skin hyperpigmentation such as spots or freckles caused by ultraviolet rays, hormones, stress, or genetic factors. do.

Another aspect of the present invention provides a cosmetic composition for improving skin troubles containing glutathione, maqui berry extract, and moth bark extract as active ingredients.

In the present invention, the term "skin trouble" means a skin disease or symptom that may be caused by inflammation caused by excessive production of nitric oxide (NO) in macrophages. The skin trouble includes all skin diseases related to inflammation regardless of their type, and non-limiting examples of skin diseases or symptoms related to the inflammation include skin rash, acne, pimple, atopic dermatitis, psoriasis, erythematous disease, skin Itching, seborrheic eczema, etc., but not limited thereto. Therefore, the term "trouble improvement" of the present invention means suppressing or inhibiting the generation of the trouble on the skin, or alleviating the trouble that has already been generated, and more specifically, suppressing the production of inflammation on the skin or already generated. It can mean "anti-inflammatory" activity that relieves or improves inflammation.

Another aspect of the present invention provides a cosmetic composition for moisturizing the skin containing glutathione, maqui berry extract, and moth extract as active ingredients.

In the present invention, the term "moisturizing the skin" means to increase the feeling of moisture in the skin and maintain a moist state.

Another aspect of the present invention provides a cosmetic composition for improving skin wrinkles comprising glutathione, a maqui berry extract, and a moth extract as active ingredients.

In the present invention, the term "wrinkle improvement" means suppressing or inhibiting the formation of wrinkles on the skin, or relieving wrinkles that have already been created, and refers to a state in which elastin and collagen are sufficiently present in the skin layer. Maintaining and enhancing skin elasticity may also be included in wrinkle improvement of the present invention.

The cosmetic composition according to the present invention may include glutathione and maqui berry extract as main components based on the Mortar bark extract, and the Mortar bark extract and main components may be included in an amount of 0.0001 to 50% by weight relative to the total weight of the cosmetic composition, preferably may be included in an amount of 0.001 to 10% by weight, more preferably 0.01 to 5% by weight.

When the content of the extract and the main component is less than 0.0001% by weight, it is difficult to expect skin whitening, skin trouble improvement, skin moisturizing and / or skin wrinkle improvement effects, and when it exceeds 50% by weight, the effect as distinct as the increasing content is Not expected.

In a preferred embodiment, the mixing ratio of glutathione, maqui berry extract, and moth extract contained in the cosmetic composition may be mixed at a weight ratio (w / w) of 1: 1 to 3: 1 to 3, 1: 1: 3 It is most preferable to mix in a weight ratio of.

When the above ingredients are within the above content range, they can bring about skin whitening, skin trouble improvement, skin moisturizing and/or skin wrinkle improvement effects with little skin irritation. It was confirmed that the cosmetic composition mixed in a ratio of 3 was the best combination.

In addition, the cosmetic composition is a softening lotion, astringent lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, skin bonding patch, skin bonding gel, powder, ointment, paste, gel, suspension, emulsion, spray, serum Or it may be formulated as a capsule, but is not particularly limited thereto.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as typical ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, A chelating agent, a colorant, a preservative, a flavoring agent, and the like may be appropriately mixed, but is not particularly limited thereto. Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, or tracanth and the like may be used.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component may be used. can be used

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleanser, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used. In addition, components included in the cosmetic composition may be included in amounts generally used in the field of skin science.

In addition, the cosmetic composition of the present invention includes commonly used adjuvants, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic actives, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, absorbents and dyes, and the like.

Another aspect of the present invention for achieving the above object provides a quasi-drug composition comprising glutathione, maqui berry extract, and moth rind extract as active ingredients. Terms such as glutathione, maqui berry, moth bark, extract, fraction, skin whitening, skin trouble, trouble improvement, anti-inflammatory, skin moisturizing and wrinkle improvement are the same as described above.

The quasi-drug composition according to the present invention may contain glutathione and maqui berry extract as main components based on the Morus burdock extract. may be included in an amount of 0.001 to 10% by weight, more preferably 0.01 to 5% by weight.

When the content of the extract and the main component is less than 0.0001% by weight, it is difficult to expect skin whitening, skin trouble improvement, skin moisturizing and / or skin wrinkle improvement effects, and when it exceeds 50% by weight, the effect as distinct as the increasing content is Not expected.

In a preferred embodiment, the mixing ratio of glutathione, maqui berry extract, and moth extract contained in the quasi-drug composition may be mixed at a weight ratio (w/w) of 1: 1 to 3: 1 to 3, and 1: 1: 3 It is most preferable to mix in a weight ratio of.

When the above ingredients are within the above content range, they can bring about skin whitening, skin trouble improvement, skin moisturizing and/or skin wrinkle improvement effects with little skin irritation. It was confirmed that the quasi-drug composition mixed in a ratio of 3 was the best combination.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, aromatics, preservatives, and the like. can include

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, laurage, and the like.

In addition, when the composition of the present invention is used as a quasi-drug, it may additionally contain one or more active ingredients exhibiting the same or similar functions. For example, it may include a known elasticity enhancing, wrinkle improving, moisturizing, or anti-inflammatory component. When an additional elasticity enhancement, wrinkle improvement, moisturizing, or anti-inflammatory component is included, the effect of improving elasticity, wrinkle improvement, moisturizing, or skin trouble improvement of the composition of the present invention may be further increased. When adding the above components, skin safety according to combined use, ease of formulation, and stability of active ingredients may be considered. The quasi-drug composition is a skin elasticity, wrinkle improvement or moisturizing component known in the art, retinoic acid, TGF, animal placenta-derived protein, betulinic acid and chlorella extract; As anti-inflammatory components known in the art, non-steroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, prednisolone, dexamethasone, allantoin, azene, hydrocortisone; And it may further include at least one component selected from the group consisting of derivatives thereof and various plant extracts. Additional components may be included in an amount of 0.0001 to 10% by weight (w/w) based on the total weight of the composition, and the content range may be adjusted according to skin safety and ease of formulation.

The quasi-drug composition of the present invention may be exemplified by a disinfectant cleanser, shower foam, ointment, wet tissue, coating agent, etc., but is not particularly limited thereto. It can be appropriately selected from conventional techniques.

In addition, the composition of the present invention, which includes glutathione and maqui berry extract as main components based on Morus burdock extract, is applied to the skin of the subject, skin whitening, trouble improvement, skin moisturizing, and / or wrinkle improvement method, etc. The subject includes, without limitation, mammals including mice, livestock, humans, and the like.

Another aspect of the present invention for achieving the above object provides a food composition comprising glutathione, maqui berry extract and moth extract as active ingredients.

The composition of the present invention, as well as skin whitening, skin trouble improvement, skin moisturizing or skin wrinkle improvement efficacy is excellent, so it is highly utilized as a health functional food composition, and can exhibit remarkably excellent effects, especially as an inner beauty food.

The food composition according to the present invention may contain glutathione and maqui berry extract as main components based on the Mortar bark extract, and the Mortar bark extract and main components may be included in an amount of 0.0001 to 50% by weight relative to the total weight of the food composition, preferably may be included in an amount of 0.001 to 10% by weight, more preferably 0.01 to 5% by weight.

When the content of the extract and the main component is less than 0.0001% by weight, it is difficult to expect skin whitening, skin trouble improvement, skin moisturizing and / or skin wrinkle improvement effects, and when it exceeds 50% by weight, the effect as distinct as the increasing content is Not expected.

In a preferred embodiment, the mixing ratio of glutathione, maqui berry extract, and moth extract contained in the food composition may be mixed at a weight ratio (w / w) of 1: 1 to 3: 1 to 3, 1: 1: 3 It is most preferable to mix in a weight ratio of.

When the above ingredients are within the above content range, they can bring about skin whitening, skin trouble improvement, skin moisturizing and/or skin wrinkle improvement effects with little skin irritation. It was confirmed that the food composition mixed in a ratio of 3 was the best combination.

The food composition of the present invention may include food additives acceptable in food science, in addition to the Mortar bark extract and main ingredients. The "food supplement additive" refers to a component that can be added to food supplementally, and can be appropriately selected and used by those skilled in the art as being added to prepare a health functional food of each formulation. Examples of food additives include nutrients, vitamins, electrolytes, flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH regulators, and stabilizers. A topical agent, a preservative, glycerin, alcohol, a carbonation agent used in carbonated beverages, and the like are included, but are not particularly limited thereto.

In addition, the food composition of the present invention may include health functional food. In the present invention, the term "health functional food" refers to food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, "functionality" means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material, and has excellent portability, so that the composition of the present invention Health functional food can be consumed as an adjuvant to enhance skin whitening, skin elasticity promotion or wrinkle improvement, skin moisturizing, antioxidant, or skin trouble improvement effect.

There are no restrictions on the form that the functional health food of the present invention can take, and it may include all foods in a conventional sense, and may be used interchangeably with terms known in the art, such as health functional food and functional food. In addition, the health functional food of the present invention may be prepared by mixing suitable other auxiliary ingredients and known additives that may be included in food according to the selection of those skilled in the art. Examples of the health functional food include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and vitamins There are complex agents, etc., and it can be prepared by adding juice, tea, jelly, juice, etc. prepared using the composition according to the present invention as a main component.

Another aspect of the present invention for achieving the above object provides a health functional food comprising the food composition. Terms such as glutathione, maqui berry, moth bark, extract, fraction, skin whitening, skin trouble, trouble improvement, anti-inflammatory, skin moisturizing, wrinkle improvement and health functional food are as described above.

The health functional food of the present invention may be characterized as an inner beauty food. In the present invention, the term "inner beauty food" is also called edible cosmetics or beauty food, and refers to food that improves skin constitution healthily by absorbing ingredients good for skin beauty and health into the body. Just like choosing cosmetics for your type, you can select and consume “inner beauty” foods that are suitable for you by considering your skin condition and lifestyle. The health functional food of the present invention has the advantage of exhibiting more excellent skin whitening, skin trouble improvement, skin moisturizing and wrinkle improvement effects by being consumed in the form of inner beauty food.

The composition containing glutathione, maqui berry extract, and moth berry extract of the present invention as active ingredients has excellent skin whitening, skin trouble improvement, skin moisturizing or skin wrinkle improvement effects, so it can be used for skin whitening, skin trouble improvement, skin moisturizing or skin It is highly applicable as a cosmetic for wrinkle improvement. In addition, the composition of the present invention can be used as a quasi-drug composition.

1 is a graph showing the melanin production inhibitory effect of the composition of the present invention.
2 is a graph showing the NO production inhibitory effect of the composition of the present invention.
Figure 3 is a graph showing the hyaluronic acid synthase (HAS3) expression effect of the composition of the present invention.
Figure 4 is a graph showing the collagen production effect of the composition of the present invention.

Hereinafter, the present invention will be described in more detail through the following examples. However, these examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited only to these examples.

Example 1: Preparation of the composition of the present invention

1-1: Manufacture of moth extract, etc.

Mortar extract was prepared through the following process, and Maqui berry extract was also prepared by performing the same process. After washing and pulverizing the moth bark, it was extracted with reflux cooling hot water for 1 hour and 30 minutes in a 20-fold volume of 30% ethanol solvent, and the extracted stock solution was filtered and concentrated under reduced pressure. The concentrate was lyophilized and used to make a dried powder. In addition, glutathione was purchased and used commercially.

1-2: Preparation of a composition containing moth bark extract, etc.

Glutathione, maqui berry extract, and moth extract prepared in Example 1-2 were respectively 1:1:3 (Preparation Example 1), 1:1:1 (Preparation Example 2), 1:3:1 (Preparation Example 3), etc. Compositions were prepared by mixing in various ratios.

Experimental Example 1: Confirmation of skin whitening effect

In order to confirm the whitening effect of the composition prepared in Example 1, the amount of melanin pigment present in melanocytes was measured and evaluated. Specifically, after treating B16 melanocytes with the composition at a concentration of 50 μg/mL and culturing for 72 hours, the amount of melanin finally produced was evaluated to evaluate the whitening effect. Melanin content was expressed as % based on DMSO control. As a positive control group, 50 μg/mL of Arbutin was used.

Referring to FIG. 1, as a result of the melanin production inhibition experiment, it can be seen that the mixture of glutathione, maqui berry, and moth bark of Example 1 exhibits an excellent whitening effect by inhibiting melanin synthesis compared to the control group. In particular, a 1:1:3 mixture of glutathione, maqui berry, and Morus cinnamon bark (Preparation Example 1) showed a synergistic effect that was superior to the whitening effect of the mixture and the control group at different ratios.

Experimental Example 2: Confirmation of skin trouble improvement effect

In order to confirm the skin trouble improving effect and anti-inflammatory effect of the composition prepared in Example 1, the NO production inhibitory effect was measured using Raw 264.7 cell. As a positive control, 20 mg/mL L-NMMA1 (NOS inhibitor) was used, and the composition was prepared at a concentration of 50 μg/mL, treated with cells, treated with 1 μg/mL LPS after 30 minutes, and cultured for 24 hours. The NO production inhibition ability was evaluated using the NO quantification kit.

Referring to FIG. 2, as a result of NO production inhibition experiment, it can be seen that the mixture of glutathione, maqui berry, and moth bark of Example 1 inhibits NO production compared to the control group and is effective in improving skin trouble. In particular, the 1:1:3 mixture of glutathione, maqui berry, and Morus cinnamon (Preparation Example 1) was 74.88% each compared to the 1:1:1 mixture (Preparation Example 2) and the 1:3:1 mixture (Preparation Example 3). , 81.04% and 87.06%, respectively, significantly inhibiting NO production.

Experimental Example 3: Confirmation of skin moisturizing effect

In order to confirm the moisturizing effect of the composition prepared in Example 1, the activity enhancing effect of hyaluronan synthases 3 (HAS3) was evaluated. Specifically, keratinocytes (HaCaT, human keratinocyte) were dispensed with 3 mL of culture medium at a density of 5 X 10 ⁵ cells/well in a 6 welll plate, and then cultured for 24 hours in a 37°C, 5% CO ₂ incubator. . Cultures were removed and washed once with PBS. The composition of Example 1 was prepared at 50 μg/mL in a serum-free culture medium, treated with 3 mL each, cultured for 24 hours, and RT-PCR was performed to compare the amount of HAS3 mRNA.

Referring to FIG. 3, in the case of a 1: 1: 3 mixture of glutathione, maqui berry, and Mortice bark (Preparation Example 1), hyaluronic acid synthases synthesis showed a statistically significant difference when compared to the control group. As a result, it can be seen that the mixture of glutathione, maqui berry, and moth bark of Example 1 exerts an excellent effect on skin moisturizing ability by inhibiting skin water loss.

Experimental Example 4: Confirmation of skin wrinkle improvement effect

In order to confirm the skin wrinkle improvement effect of the composition prepared in Example 1, the synthesis of procollagen was measured using a kit (procollagen type-1 C-peptide EIA kit, Takara, Japan).

Specifically, 1 mL of HDF (Human Dermal Fibroblast) cells were dispensed into a 24-well plate at a density of 5 × 10 ⁴ cell/mL, and treated with a concentration of 50 μg/mL of the composition of Example 1 at 37° C., 5% CO ₂ Incubated for 48 hours in an incubator. Add 100 μL of antibody-POD conjugate sol'n to a 96-well plate coated with anti-PIP antibody, add 20 μL of standard solution to the control and the above composition, block light with aluminum foil, and for 3 hours Incubated at 37°C. Then, the culture solution was removed, washed 4 times with PBS, 100 μL of substrate solution was added, left at room temperature for 15 minutes, 100 μL of H ₂ SO ₄ was added, shaken lightly, and absorbance was measured at 450 nm.

Referring to Figure 4, glutathione, maqui berry, 1: 1: 3 mixture (Preparation Example 1) shows a significant synergistic effect on the synthesis of Procollagen compared to the control group. As a result, the mixture of glutathione, maqui berry, and moth bark of Example 1 can increase the synthesis of procollagen, thereby improving skin wrinkles.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (13)

Contains glutathione, maqui berry extract, and moth berry extract as active ingredients, and the mixing ratio of the glutathione, maqui berry extract, and moth berry extract is mixed at a weight ratio of 1: 1: 3 based on weight ratio (w / w) Characterized in, cosmetic composition. The cosmetic composition according to claim 1, wherein the composition is for skin whitening. The cosmetic composition according to claim 1, wherein the composition is for improving skin troubles. The cosmetic composition according to claim 1, wherein the composition is for moisturizing the skin. The cosmetic composition according to claim 1, wherein the composition is for improving skin wrinkles. It contains glutathione, maqui berry extract and moth berry extract as active ingredients, and the mixing ratio of the glutathione, maqui berry extract and moth berry extract is 1: 1: 3 based on weight ratio (w / w). , Quasi-drug composition. The quasi-drug composition according to claim 6, wherein the composition is for skin whitening. The quasi-drug composition according to claim 6, wherein the composition is for improving skin troubles. The quasi-drug composition according to claim 6, wherein the composition is for moisturizing the skin. The quasi-drug composition according to claim 6, wherein the composition is for improving skin wrinkles. It contains glutathione, maqui berry extract and moth berry extract as active ingredients, and the mixing ratio of the glutathione, maqui berry extract and moth berry extract is 1: 1: 3 based on weight ratio (w / w). , food composition. The food composition according to claim 11, wherein the composition is a health functional food. It contains glutathione, maqui berry extract and moth berry extract as active ingredients, and the mixing ratio of the glutathione, maqui berry extract and moth berry extract is 1: 1: 3 based on weight ratio (w / w). , Inner beauty composition.

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