KR102182710B1 - Compositions for Skin Moisture Comprising Extract of Plectranthus tomentosa or Complex Extract thereof - Google Patents
Compositions for Skin Moisture Comprising Extract of Plectranthus tomentosa or Complex Extract thereof Download PDFInfo
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- KR102182710B1 KR102182710B1 KR1020190043544A KR20190043544A KR102182710B1 KR 102182710 B1 KR102182710 B1 KR 102182710B1 KR 1020190043544 A KR1020190043544 A KR 1020190043544A KR 20190043544 A KR20190043544 A KR 20190043544A KR 102182710 B1 KR102182710 B1 KR 102182710B1
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- KR
- South Korea
- Prior art keywords
- extract
- tea tree
- skin
- moisturizing
- centella asiatica
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Classifications
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
본 발명은 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 유효성분으로 포함하는 피부 보습용 화장료 조성물에 관한 것이다.
본 발명의 피부 보습용 조성물은 히알루론산(Hyaluronic acid)의 생성을 증가시키고, 필라그린(Filaggrin) 및 히알루론산 합성효소(HAS-3, hyaluronan synthase-3) 유전자 발현을 촉진시켜, 이로 인해 우수한 보습 효과가 있으므로, 화장품, 식품, 및 의약외품 분야에서 피부 보습 목적으로 유용하게 이용가능하다. 또한, 본 발명의 조성물은 인체에 매우 안전할 뿐만 아니라, 안정성도 매우 탁월하다.The present invention relates to a cosmetic composition for moisturizing skin comprising a single extract of rose herb, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as an active ingredient.
The composition for moisturizing skin of the present invention increases the production of hyaluronic acid and promotes gene expression of filaggrin and hyaluronic acid synthase (HAS-3, hyaluronan synthase-3), thereby excellent moisturizing Since it is effective, it can be usefully used for skin moisturizing purposes in cosmetics, food, and quasi-drug fields. In addition, the composition of the present invention is not only very safe for the human body, but also has excellent stability.
Description
본 발명은 피부 보습용 조성물에 관한 것으로, 더욱 구체적으로 장미허브 추출물, 또는 이의 복합 추출물을 유효성분으로 함유하는 피부 보습용 조성물을 유효성분으로 함유하는 피부 보습용 조성물에 관한 것이다.The present invention relates to a composition for moisturizing the skin, and more particularly, to a composition for moisturizing skin containing a composition for moisturizing the skin containing a rose herb extract or a complex extract thereof as an active ingredient.
일반적으로 피부에 사용되는 보습용 화장료 조성물은 사람의 모발이나 피부 등에 수분을 일정량 유지함으로써 부드럽고 생기있게 하며, 갈라짐, 건조함 등의 손상을 방지해주는 역할을 한다. 즉, 피부 보습용 화장료 조성물은 피부나 모발에 일정량 이상의 수분을 공급하거나 수분이 유지함으로써 피부나 모발을 미화하고, 건강하게 유지하기 위한 목적으로 사용된다. In general, moisturizing cosmetic compositions used for skin make it soft and vital by maintaining a certain amount of moisture in human hair or skin, and prevent damage such as cracking and dryness. That is, the cosmetic composition for moisturizing skin is used for the purpose of beautifying skin or hair and maintaining healthy skin or hair by supplying or maintaining moisture in a certain amount or more to the skin or hair.
피부는 수분 유지에 필수적인 기능을 하는 기관으로, 수분 조절에 대략 2/3 정도의 비중을 담당하는 것으로 알려져 있다. 따라서, 화장품 분야에서는 수분의 공급, 유지, 즉 보습과 관련된 기능성 연구가 활발하게 진행되고 있다. 또한, 최근에는 천연물 등으로부터 얻은 생리 활성 물질을 화장료에 도입하여 피부 자체의 고유 방어 기능을 더욱 강화함으로써 피부의 보습력을 증가시키는 화장품의 개발이 활발하게 진행되는 추세이다.The skin is an organ that plays an essential function in maintaining moisture, and it is known to be responsible for about 2/3 of the weight in moisture control. Therefore, in the field of cosmetics, functional studies related to supply and maintenance of moisture, that is, moisturizing, are actively being conducted. In addition, in recent years, the development of cosmetics that increase the moisturizing power of the skin by introducing physiologically active substances obtained from natural products into cosmetics to further strengthen the skin's own defense function has been actively progressed.
피부 보습 효과가 있는 천연소재기술과 관련하여, 특허공개공보 제10-2015-0045049호 ‘식물 추출물을 함유하는 피부 보습용 화장료 조성물 및 이의 제조방법’은 편백잎 액상 추출물, 솔잎 액상 추출물, 잣나무잎 액상 추출물, 녹차잎 액상 추출물 및 병풀 액상 추출물의 발효 추출물이 갖는 피부보습 효과를 개시한 바 있고, 특허공개 제10-2017-0130865호 ‘식물 추출물을 포함하는 피부 항염 또는 피부 보습용 조성물’은 피버퓨 추출물, 꿀풀 추출물 및 오이풀 추출물 등을 조합한 성분이 피부 항염 또는 피부 보습에 효과가 있음을 개시한 바 있다. Regarding natural material technology with skin moisturizing effect, Patent Laid-Open Publication No. 10-2015-0045049'A cosmetic composition for moisturizing skin containing plant extracts and its manufacturing method' refers to liquid extract of cypress leaves, liquid extract of pine needles, and pine needles. The skin moisturizing effect of the fermented extract of liquid extract, green tea leaf liquid extract and centella asiatica liquid extract has been disclosed, and Patent Publication No. 10-2017-0130865'A composition for skin anti-inflammatory or skin moisturizing containing plant extracts' is Fever. It has been disclosed that a combination of Pew extract, Lamiaceae extract, and cucumber grass extract is effective in skin anti-inflammatory or skin moisturizing.
그러나, 복합 식물추출물을 이용한 다양한 생리활성 연구는 아직 미흡한 실정이고, 특히 산업현장에서 적용 가능하도록 충분한 보습 효과를 갖는 식물 복합추출물에 대한 연구가 미진하다. 또한, 기능성 화장품 또는 식품 등의 원료로서 개발이 가능하도록 충분하게 보습 효과를 갖는 식물 추출물의 조합에 관한 연구 결과는 아직까지 거의 없는 실정이다.However, studies on various physiological activities using complex plant extracts are still insufficient, and studies on plant complex extracts having sufficient moisturizing effect to be applicable in industrial sites are not yet insufficient. In addition, there are few research results on the combination of plant extracts having sufficient moisturizing effect so that they can be developed as raw materials for functional cosmetics or foods.
이러한 배경 하에서, 본 발명자들은 피부 보습 효과가 우수한 천연물질의 조합에 관하여 연구를 수행하였으며, 장미허브 추출물이 우수한 피부 보습 효과를 갖고 있음을 일차적으로 확인한 후, 이를 추가적으로 병풀 추출물, 티트리 추출물, 및 마치현 추출물과 조합한 복합 추출물이 실제 산업현장에서 적용할 수 있는 정도로 탁월한 보습 효과를 나타냄을 규명하여, 본 발명을 완성하게 되었다.Under this background, the present inventors conducted a study on the combination of natural substances having excellent skin moisturizing effect, and after first confirming that the rose herb extract has an excellent skin moisturizing effect, it was additionally centella extract, tea tree extract, and The present invention was completed by finding out that the complex extract combined with the extract of the machisuki shows an excellent moisturizing effect to the extent that it can be applied in an actual industrial field.
따라서, 본 발명의 주된 목적은 장미허브 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 유효성분으로 포함하는 피부 보습용 화장료 조성물을 제공하는 것이다.Accordingly, the main object of the present invention is to provide a cosmetic composition for skin moisturizing comprising a rose herb extract, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as an active ingredient.
본 발명의 다른 목적은 장미허브 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 유효성분으로 포함하는 피부 보습용 식품 조성물을 제공하는 것이다.Another object of the present invention is to provide a food composition for moisturizing the skin comprising a rose herb extract, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as an active ingredient.
본 발명의 또 다른 목적은 장미허브 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 유효성분으로 포함하는 피부 보습용 의약외품 조성물을 제공하는 것이다.Another object of the present invention is to provide a quasi-drug composition for skin moisturizing comprising a rose herb extract, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as an active ingredient.
본 발명의 다른 목적 및 이점은 하기의 발명의 상세한 설명, 청구범위 및 도면에 의해 보다 명확하게 된다.Other objects and advantages of the present invention will become more apparent by the following detailed description, claims and drawings.
본 발명의 한 양태에 따르면, 본 발명은 장미허브 추출물을 유효성분으로 포함하는 피부 보습용 화장료 조성물을 제공한다.According to one aspect of the present invention, the present invention provides a skin moisturizing cosmetic composition comprising a rose herb extract as an active ingredient.
또한, 본 발명의 한 양태에 따르면, 본 발명은 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 유효성분으로 포함하는 피부 보습용 화장료 조성물을 유효성분으로 포함하는 피부 보습용 화장료 조성물을 제공한다.In addition, according to an aspect of the present invention, the present invention provides a cosmetic composition for moisturizing skin comprising a cosmetic composition for moisturizing skin comprising a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as an active ingredient. do.
본 발명자들은 피부 보습 효과가 우수한 물질의 조합에 관하여 연구를 수행하였고, 여러 식물체 중 장미허브 추출물이 가장 우수한 피부 보습 효과가 있음을 확인하였다. 또한, 장미허브 추출물에 병풀 추출물, 티트리 추출물, 및 마치현 추출물을 조합하여 제조한 복합 추출물이 종래 장미허브 단일 추출물과 대비하여 인간각질형성세포에서 히알루론산의 생성을 유의하게 증가시키고, 보습 관련 유전자인 Filaggrin 및 Hyaluronan synthase-3 유전자의 발현을 유의하게 증가시킴으로써, 더욱 우수한 보습 효과가 있음을 증명하였다.The present inventors conducted a study on the combination of substances having excellent skin moisturizing effect, and it was confirmed that rose herb extract among various plants has the best skin moisturizing effect. In addition, the complex extract prepared by combining the rose herb extract with centella asiatica extract, tea tree extract, and machisuki extract significantly increased the production of hyaluronic acid in human keratinocytes compared to the conventional rose herb single extract, and moisturizing-related genes By significantly increasing the expression of the Filaggrin and Hyaluronan synthase-3 genes, it was demonstrated that there is a more excellent moisturizing effect.
본 발명의 "장미허브(Plectranthus tomentosa)"는 쌍떡잎식물 꿀풀과의 다육식물을 의미하고, "병풀(Centella asiatica)"은 쌍떡잎식물 이판화군 산형화목 미나리과의 여러해살이풀을 의미하며, "티트리(Melaleuca alternifolia)"는 쌍떡잎식물 도금양목 도금양과의 허브를 의미하고, "마치현(Portulaca oleracea L.)"은 쇠비름과의 쇠비름의 전초를 의미한다. The "rose herb ( Plectranthus) of the present invention tomentosa )" means a succulent plant of the dicotyledonous family Lamiaceae, " Centella asiatica " means a perennial plant of the umbel family of the dicotyledons Ipanhwa group, and "Tea tree ( Melaleuca alternifolia )" is a dicotyledon plating Yangmok means the herb of the family of gildedyang, and "Machihyeon ( Portulaca oleracea L.)" means the outpost of the purslane family.
본 발명의 상기 장미허브, 병풀, 티트리, 및 마치현은, 이들의 천연, 잡종 또는 변종 식물로부터 추출될 수 있고, 식물 조직 배양물에서도 추출할 수 있다. The rose herb, centella asiatica, tea tree, and masquerade of the present invention can be extracted from their natural, hybrid or variety plants, and can also be extracted from plant tissue culture.
본 발명에서 사용되는 용어, "피부 보습" 이란 피부에 수분감을 증가시켜주고, 촉촉한 상태를 유지시키는 것을 의미한다. 본 발명의 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물은 우수한 수분 보유 효과를 나타내었는바, 이로부터 본 발명의 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 포함하는 조성물은 피부 보습 용도로 사용될 수 있음을 확인하였다.The term "skin moisturizing" as used in the present invention means to increase the moisture sensation on the skin and maintain a moist state. The rose herb extract of the present invention, centella asiatica extract, tea tree extract, and masquerade extract exhibited excellent water retention effect, from which the rose herb single extract of the present invention, or rose herb, centella asiatica, tea tree, and a combination of It was confirmed that the composition containing the extract can be used for skin moisturizing purposes.
본 발명에서 사용되는 용어, "추출물"은 식물의 추출 처리로 얻어지는 추출액, 상기 추출액의 희석액이나 농축액, 상기 추출액을 건조하여 얻어지는 건조물, 상기 추출액의 조정제물이나 정제물, 또는 이들의 혼합물 등, 추출액 자체 및 추출액을 이용하여 형성 가능한 모든 제형의 추출물을 포함한다. 본 발명의 상기 추출물은 바람직하게 추출 후 건조 분말 형태로 제조되어 사용될 수 있다. The term "extract" as used in the present invention refers to an extract obtained by extracting a plant, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof, etc. It includes extracts of all formulations that can be formed using itself and extracts. The extract of the present invention may be preferably prepared and used in the form of a dry powder after extraction.
본 발명의 상기 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물의 추출에 있어서, 상기 추출물을 추출하는 방법은 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 추출할 수 있다. 상기 추출방법의 비제한적인 예로는, 열수 추출법, 초음파 추출법, 여과법, 환류 추출법 등을 들 수 있으며, 이들은 단독으로 수행되거나 2종 이상의 추출방법을 병용하여 수행될 수 있다. In the extraction of the single extract of rose herb of the present invention, or the complex extract of rose herb, centella asiatica, tea tree, and masquerade, the method of extracting the extract is not particularly limited, and a method commonly used in the art You can extract accordingly. Non-limiting examples of the extraction method may include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more extraction methods.
본 발명에서 상기 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 추출하는데 사용되는 추출 용매의 종류는 특별히 제한되지 아니하며, 당해 기술 분야에서 공지된 임의의 용매를 사용할 수 있다. 상기 추출 용매의 비제한적인 예로는 물; 메탄올, 에탄올, 프로필알코올, 부틸알코올 등의 C1 내지 C4의 저급 알코올; 글리세린, 부틸렌글리콜, 프로필렌글리콜 등의 다가 알코올; 및 메틸아세테이트, 에틸아세테이트, 아세톤, 벤젠, 헥산, 디에틸에테르, 디클로로메탄 등의 탄화수소계 용매; 또는 이들의 혼합물을 사용할 수 있으며, 바람직하게, 물, 저급알코올, 1,3-부틸렌글리콜, 에틸아세테이트를 단독으로 사용하거나 2종 이상 혼합하여 사용할 수 있다. In the present invention, the type of the extraction solvent used to extract the single extract of rose herb, or the complex extract of rose herb, centella asiatica, tea tree, and machihyun is not particularly limited, and any solvent known in the art may be used. have. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more.
본 발명에서 열수 추출 또는 냉침 추출한 추출물은 부유하는 고체 입자를 제거하기 위해 여과, 예를 들어 나일론 등을 이용해 입자를 걸러내거나 냉동여과법 등을 이용해 여과한 후, 그대로 사용하거나 이를 동결건조, 열풍건조, 분무건조 등을 이용해 건조하여 사용할 수 있다.In the present invention, the extract extracted with hot water or cold needle is filtered to remove floating solid particles, for example, nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., or use as such, or freeze drying, hot air drying, It can be used after drying by spray drying or the like.
또한, 본 발명의 상기 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물이 함유된 피부 보습용 화장료 조성물에서, 전체 조성물 100 중량부 대비 상기 장미허브 추출물은 0.1 내지 20 중량부로 포함될 수 있고, 상기 병풀 추출물은 0.01 내지 2.5 중량부, 상기 티트리 추출물은 0.01 내지 2.5 중량부, 및 상기 마치현 추출물은 0.01 내지 2.5 중량부로 포함될 수 있다. In addition, in the cosmetic composition for moisturizing the skin containing a single extract of the rose herb of the present invention, or a complex extract of rose herb, centella, tea tree, and macula, the rose herb extract is 0.1 to 20 parts by weight based on 100 parts by weight of the total composition. It may be included in parts, the centella asiatica extract may be included in an amount of 0.01 to 2.5 parts by weight, the tea tree extract in an amount of 0.01 to 2.5 parts by weight, and the portulaca oleracea extract may be included in an amount of 0.01 to 2.5 parts by weight.
또한, 본 발명의 상기 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물이 함유된 피부 보습용 화장료 조성물에서, 상기 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물은 5-15:0.5-2:0.5-2:0.5-2의 중량비로 혼합되는 것이 바람직하고, 10:1:1:1의 중량비로 혼합되는 것이 가장 바람직하다. 본 발명의 실시예에서는 상기 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 포함하는 피부 보습용 조성물이 세포 독성이 없고, 히알루론산의 합성을 증가하여 피부 보습에 개선 효과가 있음을 확인할 수 있었다. 특히, 상기 추출물들을 다양한 비율로 혼합하여 피부 보습 효과를 확인한 결과, 장미허브, 병풀, 티트리, 및 마치현이 10:1:1:1의 비율로 혼합된 복합 추출물에서 피부 보습에 가장 높은 시너지 효과가 있음을 확인할 수 있었다.In addition, in the cosmetic composition for moisturizing the skin containing the rose herb, centella asiatica, tea tree, and the complex extract of machi-hyun of the present invention, the rose-herb extract, centella asiatica extract, tea tree extract, and machi-hyun extract are 5-15:0.5 It is preferably mixed in a weight ratio of -2:0.5-2:0.5-2, and most preferably mixed in a weight ratio of 10:1:1:1. In an embodiment of the present invention, it can be confirmed that the composition for skin moisturizing comprising the complex extract of rose herb, centella asiatica, tea tree, and masquerade has no cytotoxicity, and has an improving effect on skin moisturizing by increasing the synthesis of hyaluronic acid. there was. In particular, as a result of confirming the skin moisturizing effect by mixing the above extracts in various ratios, the highest synergistic effect on skin moisturizing in the complex extract in which rose herb, centella asiatica, tea tree, and masquerade were mixed in a ratio of 10:1:1:1 It was confirmed that there is.
본 발명의 상기 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물은 우수한 피부 보습 효과를 나타내는데, 구체적으로 히알루론산(Hyaluronic acid)의 합성을 촉진하고, 필라그린(Filaggrin) 및 히알루론산 합성효소(HAS-3, hyaluronan synthase-3) 유전자의 발현을 증가시키는 것을 특징으로 한다. The single extract of the rose herb of the present invention, or the complex extract of rose herb, centella, tea tree, and machihyun exhibit excellent skin moisturizing effect, specifically promoting the synthesis of hyaluronic acid, and filaggrin And hyaluronic acid synthase (HAS-3, hyaluronan synthase-3) characterized by increasing the expression of the gene.
본 발명의 피부 보습용 화장료 조성물에서, 상기 조성물은 용액, 외용연고, 크림, 폼, 영양화장수, 유연화장수, 마스크 팩, 유연수, 유액, 메이크업베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린크림, 선오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 패치 및 스프레이로 구성된 군으로부터 선택되는 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.In the skin moisturizing cosmetic composition of the present invention, the composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, mask pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen Cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray It can be, but is not limited thereto.
또한, 본 발명의 화장료 조성물은 일반 피부 화장료에 배합되는 화장품학적으로 허용 가능한 담체를 1종 이상 추가로 포함할 수 있으며, 통상의 성분으로 예를 들면 유분, 물, 계면활성제, 보습제, 저급 알콜, 증점제, 킬레이트제, 색소, 방부제, 향료 등을 적절히 배합할 수 있으나, 이에 제한되는 것은 아니다. In addition, the cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately mixed, but the present invention is not limited thereto.
본 발명의 화장료 조성물에 포함되는 화장품학적으로 허용 가능한 담체는 제형에 따라 다양하다.The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.
본 발명의 제형이 연고, 페이스트, 크림 또는 젤인 경우에는, 담체 성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화아연 또는 이들의 혼합물이 이용될 수 있다.When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these can be used.
본 발명의 제형이 파우더 또는 스프레이인 경우에는, 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록사이드, 칼슘 실케이트, 폴리아미드 파우더 또는 이들의 혼합물이 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸에테르와 같은 추진제를 포함할 수 있다.When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component, and in particular, in the case of a spray, additional chloro Propellants such as fluorohydrocarbons, propane/butane or dimethylether may be included.
본 발명의 제형이 용액 또는 유탁액인 경우에는, 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되며, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알콜, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일이 이용될 수 있으며, 특히, 목화씨 오일, 땅콩 오일, 옥수수 배종 오일, 올리브오일, 피마자 오일 및 참깨 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 이용될 수 있다.When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used. have.
본 발명의 제형이 현탁액인 경우에는, 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알콜, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.
본 발명의 제형이 비누인 경우에는 담체 성분으로서 지방산의 알칼리 금속 염, 지방산 헤미에스테르 염, 지방산 단백질 히드롤리제이트, 이세티오네이트, 라놀린 유도체, 지방족 알콜, 식물성 유, 글리세롤, 당 등이 이용될 수 있다.When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. may be used as a carrier component. I can.
본 발명의 다른 양태에 따르면, 본 발명은 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물에서 선택된 어느 하나를 유효성분으로 포함하는 피부 보습용 식품 조성물을 제공한다. 본 발명의 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물과, 피부 보습은 상기에서 설명한 바와 같다. 상기 식품 조성물은 건강기능식품의 형태로 사용될 수 있으나, 이에 제한되는 것은 아니다.According to another aspect of the present invention, the present invention provides a food composition for skin moisturizing, comprising, as an active ingredient, any one selected from a single extract of rose herb, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun. The rose herb extract, centella asiatica extract, tea tree extract, and masquerade extract of the present invention, and skin moisturizing are as described above. The food composition may be used in the form of a health functional food, but is not limited thereto.
본 발명의 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 포함하는 식물 추출물, 이의 분획물 또는 이의 가공물의 형태로 포함될 수 있다. 또한, 상기 조성물은 유효성분 이외에 식품학적으로 허용 가능한 식품보조첨가제를 포함할 수 있다.It may be included in the form of a single extract of rose herb of the present invention, or a plant extract including a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun, a fraction thereof, or a processed product thereof. In addition, the composition may contain food additives that are acceptable food additives in addition to the active ingredient.
본 발명에 있어서, "식품보조첨가제"란 식품에 보조적으로 첨가될 수 있는 구성요소를 의미하며, 각 제형의 건강기능식품을 제조하는데 첨가되는 것으로서 당업자가 적절히 선택하여 사용할 수 있다. 식품보조첨가제의 예로는 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 충진제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등이 포함되지만, 상기 예들에 의해 본 발명의 식품보조첨가제의 종류가 제한되는 것은 아니다.In the present invention, the term "food supplementary additive" refers to a component that can be added auxiliary to food, and is added to the manufacture of health functional foods of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavors, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.
본 발명의 식품 조성물에는 건강기능식품이 포함될 수 있다. 본 발명에 있어서, "건강기능식품"이란 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 정제, 캅셀, 분말, 과립, 액상 및 환 등의 형태로 제조 및 가공한 식품을 말한다. 여기서 “기능성”이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻는 것을 의미한다. 본 발명의 건강기능식품은 당업계에서 통상적으로 사용되는 방법으로 제조 가능하며, 상기 제조시에는 당업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. The food composition of the present invention may contain a health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, the term “functionality” means obtaining useful effects for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and during the production, raw materials and ingredients commonly added in the art may be added.
또한, 상기 건강기능식품의 제형 또한 건강기능식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 본 발명의 식품 조성물은 다양한 형태의 제형으로 제조될 수 있으며, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고, 휴대성이 뛰어나, 본 발명의 건강기능식품은 피부 보습 효과를 증진시키기 위한 보조제로 섭취가 가능하다.In addition, the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be prepared in various forms of formulation, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability. Health functional foods can be consumed as supplements to enhance the skin moisturizing effect.
본 발명의 건강기능식품이 취할 수 있는 형태에는 제한이 없으며, 통상적인 의미의 식품을 모두 포함할 수 있고, 기능성 식품 등 당업계에 알려진 용어와 혼용이 가능하다. 아울러 본 발명의 건강기능식품은 당업자의 선택에 따라 식품에 포함될 수 있는 적절한 기타 보조 성분과 공지의 첨가제를 혼합하여 제조할 수 있다. 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올 음료 및 비타민 복합제 등이 있으며, 본 발명에 따른 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 주성분으로 하여 제조한 즙, 차, 젤리 및 주스 등에 첨가하여 제조할 수 있다. 또한, 동물을 위한 사료로 이용되는 식품도 포함한다.There is no limit to the form that the health functional food of the present invention can take, and may include all foods in a conventional sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing appropriate other auxiliary ingredients and known additives that may be included in the food according to the choice of a person skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes and the like, and it can be prepared by adding a single extract of rose herb according to the present invention, or juice, tea, jelly, juice, etc., prepared by using a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as main components. It also includes foods used as feed for animals.
또한, 본 발명의 다른 양태에 따르면, 본 발명은 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물에서 선택된 어느 하나를 유효성분으로 포함하는 피부 보습용 의약외품 조성물을 제공한다. 본 발명의 상기 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물과, 피부 보습은 상기에서 설명한 바와 같다.In addition, according to another aspect of the present invention, the present invention provides a quasi-drug composition for skin moisturizing comprising, as an active ingredient, any one selected from a single extract of rose herb, or a complex extract of rose herb, centella asiatica, tea tree, and macula. . The single extract of the rose herb of the present invention, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun, and skin moisturizing are as described above.
본 발명의 의약외품 조성물에는 상기 성분 외에 필요에 따라 약학적으로 허용 가능한 담체, 부형제 또는 희석제를 더욱 포함할 수 있다. 상기 약학적으로 허용 가능한 담체, 부형제 또는 희석제는 본 발명의 효과를 해하지 않는 한 제한되지 않으며, 예를 들어 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제, 윤활제, 감미제, 방향제, 보존제 등을 포함할 수 있다.The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent as needed in addition to the above ingredients. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. Can include.
본 발명의 약학적으로 허용 가능한 담체, 부형제 또는 희석제의 대표적인 예로는, 락토즈, 덱스트로스, 슈크로스, 솔비톨, 만니톨, 자일리톨, 말티톨, 전분, 젤라틴, 글리세린, 아카시아 고무, 알지네이트, 칼슘포스페이트, 칼슘카보네이트, 칼슘실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트, 광물유, 프로필렌글리콜, 폴리에틸렌글리콜, 식물성 오일, 주사가능한 에스테르, 위텝솔, 마크로골, 트윈 61, 카카오지, 라우리지 등을 들 수 있다.Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , Injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, and laurage.
또한, 본 발명의 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 의약외품으로 사용하는 경우, 추가로 동일 또는 유사한 기능을 나타내는 유효성분을 1종 이상 함유할 수 있다. 예컨대, 공지의 보습 성분을 포함할 수 있을 것이다. 추가적인 보습 성분을 포함하게 되면 본 발명 조성물의 보습 효과는 더욱 증가할 수 있다. 상기 성분 추가 시에는 복합 사용에 따른 피부 안전성, 제형화의 용이성, 유효성분들의 안정성을 고려할 수 있다. 상기 의약외품 조성물은 당업계에 공지된 보습 성분으로서, 레티놀산, TGF, 동물 태반 유래의 단백질, 베툴린산 및 클로렐라 추출물; 및 이들의 유도체와 각종 식물추출물로 구성되는 군으로부터 선택되는 1종 또는 2종 이상의 성분을 추가로 포함할 수 있다. 추가 성분은 전체 조성물 중량에 대하여 0.001 중량% 내지 10중량%로 포함될 수 있을 것이며, 상기 함량 범위는 피부 안전성과, 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물의 제형화 시의 용이성 등의 요건에 따라 조절될 수 있을 것이다.In addition, when a single extract of rose herb of the present invention, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun is used as a quasi-drug, it may further contain one or more active ingredients showing the same or similar function. For example, a known moisturizing component may be included. When the additional moisturizing component is included, the moisturizing effect of the composition of the present invention may be further increased. When the above ingredients are added, skin safety, ease of formulation, and stability of active ingredients can be considered in combination with use. The quasi-drug composition is a moisturizing component known in the art, including retinoleic acid, TGF, protein derived from animal placenta, betulinic acid and chlorella extract; And it may further include one or more components selected from the group consisting of derivatives and various plant extracts thereof. The additional ingredients may be included in an amount of 0.001% to 10% by weight based on the total weight of the composition, and the content range is a formulation of a single extract of rose herb, or a complex extract of rose herb, centella asiatica, tea tree, and masquerade. It may be adjusted according to requirements such as ease of use.
본 발명의 의약외품 조성물은 소독 청결제, 샤워폼, 연고액, 물티슈, 코팅제 등을 예시할 수 있으나 이에 제한되는 것이 아니며, 의약외품의 제제화 방법, 용량, 이용방법, 구성성분 등은 기술 분야에 공지된 통상의 기술로부터 적절히 선택될 수 있다.The quasi-drug composition of the present invention may exemplify a disinfectant cleaner, a shower foam, an ointment, a wet tissue, a coating agent, etc., but is not limited thereto, and the method of formulating a quasi-drug, a dosage, a method of use, a component, etc., are known in the art. It can be appropriately selected from the techniques of.
또한, 본 발명의 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물은 개체의 피부에 도포하는 단계를 포함하는, 피부 보습 방법에 사용될 수 있다. 상기 개체는 쥐, 가축, 인간 등을 포함하는 포유동물을 제한 없이 포함한다.In addition, a single extract of rose herb of the present invention, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyeon may be used in a skin moisturizing method, including the step of applying to the skin of an individual. The subject includes, without limitation, mammals including mice, livestock, humans, and the like.
본 발명의 특징 및 이점을 요약하면 다음과 같다:The features and advantages of the present invention are summarized as follows:
(i) 본 발명은 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 유효성분으로 포함하는 피부 보습용 조성물을 제공한다.(i) The present invention provides a composition for moisturizing skin comprising a single extract of rose herb, or a complex extract of rose herb, centella asiatica, tea tree, and machi-hyun as an active ingredient.
(ii) 본 발명의 피부 보습용 조성물은 히알루론산(Hyaluronic acid)의 합성을 촉진하고, 필라그린(Filaggrin) 및 히알루론산 합성효소(HAS-3, hyaluronan synthase-3) 유전자의 발현을 증가시켜 우수한 보습 효과가 있으므로, 화장품, 식품, 및 의약외품 분야에서 피부 보습 목적으로 유용하게 이용 가능하다.(ii) The composition for moisturizing skin of the present invention promotes the synthesis of hyaluronic acid, and increases the expression of filaggrin and hyaluronic acid synthase (HAS-3, hyaluronan synthase-3) genes. Since it has a moisturizing effect, it can be usefully used for skin moisturizing purposes in cosmetics, food, and quasi-drug fields.
(iii) 또한, 본 발명의 조성물은 인체에 매우 안전할 뿐만 아니라, 안정성도 매우 탁월하다.(iii) In addition, the composition of the present invention is not only very safe for the human body, but also very excellent in stability.
도 1은 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물을 단독으로 인간각질형성세포(HaCaT)에 처리한 후, 세포 생존율의 변화를 측정하여 나타낸 그래프이다.
도 2는 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물을 각각 단독으로 인간각질형성세포에 처리한 후, 히알루론산의 생성량 변화를 측정하여 나타낸 그래프이다.
도 3은 조합 비율을 달리하여 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 인간각질형성세포에 처리한 후, 히알루론산의 생성량 변화를 측정하여 나타낸 그래프이다.
도 4는 조합 비율을 달리하여 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 인간각질형성세포에 처리한 후, 히알루론산의 생성량 변화를 측정하여 나타낸 그래프이다.
도 5는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물(10:1:1:1 중량비)을 인간각질형성세포에 처리한 후, 보습 관련 유전자인 필라그린(Filaggrin) 유전자의 발현량을 측정하여 나타낸 그래프이다.
도 6은 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물(10:1:1:1 중량비)을 인간각질형성세포에 처리한 후, 보습 관련 유전자인 히알루론산 합성효소(HAS-3, hyaluronan synthase-3) 유전자의 발현량을 측정하여 나타낸 그래프이다.FIG. 1 is a graph showing changes in cell viability after treatment with rose herb extract, centella asiatica extract, tea tree extract, and masquerade extract alone on human keratinocytes (HaCaT).
FIG. 2 is a graph showing changes in the amount of hyaluronic acid produced after each treatment of human keratinocytes with rose herb extract, centella asiatica extract, tea tree extract, and masquerade extract alone.
3 is a graph showing changes in the amount of hyaluronic acid produced after treating human keratinocytes with complex extracts of rose herb, centella asiatica, tea tree, and machi-hyun at different combinations.
FIG. 4 is a graph showing changes in the amount of hyaluronic acid produced after treating human keratinocytes with complex extracts of rose herb, centella asiatica, tea tree, and machi-hyun at different combinations.
Figure 5 shows the expression level of the moisturizing-related gene Filaggrin gene after treatment of the complex extracts (10:1:1:1 by weight) of rose herb, centella asiatica, tea tree, and machi-hyun on human keratinocytes. This is a graph that was measured and shown.
Fig. 6 is a hyaluronic acid synthase (HAS-3, hyaluronan) that is a moisturizing-related gene after treating human keratinocytes with complex extracts (10:1:1:1 by weight) of rose herb, centella asiatica, tea tree, and machis. This is a graph showing the expression level of synthase-3) gene.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail through examples. Since these examples are for illustrative purposes only, the scope of the present invention is not to be construed as being limited by these examples.
실시예Example 1. One. 장미허브Rose herb , , 병풀Centella , , 티트리Tea tree , 및 , And 마치현Machi Prefecture 추출물의 제조 Preparation of extract
장미허브, 병풀, 티트리, 및 마치현의 전초 0.5kg을 정제수로 세척하고 건조시킨 다음, 분쇄하고 세말화하여 분말을 얻었다. 분말화된 장미허브는, 병풀, 티트리, 및 마치현에 각각 10배 중량의 정제수를 가한 후, 하룻밤 동안 교반시키며 정제수에 추출한 후, 여과하여 그 상등액을 동결건조하여 추출물을 수득하였다.Rose herb, centella asiatica, tea tree, and 0.5 kg of outpost of machi-hage were washed with purified water, dried, pulverized and finely divided to obtain powder. Powdered rose herb was added 10 times the weight of purified water to Centella asiatica, tea tree, and portulaca, and then stirred overnight, extracted with purified water, filtered, and lyophilized to obtain an extract.
실시예Example 2. 2. 장미허브Rose herb , , 병풀Centella , , 티트리Tea tree , 및 , And 마치현의Machi prefecture 단일추출물에 대한 세포 독성 효과 검증 Verification of cytotoxic effect on single extract
장미허브, 병풀, 티트리, 및 마치현 추출물이 인간각질형성세포(HaCaT, Human immortalized keratinocyte)의 생장에 미치는 효과를 아래와 같은 방법으로 확인하였다. The effect of extracts of rose herb, centella asiatica, tea tree, and mackerel on the growth of human immortalized keratinocytes (HaCaT) was confirmed by the following method.
우선, 10% FBS(fetal bovine serum, Cambrex)가 포함된 전용 배지인 DMEM(Dulbeccos modified Eagles medium) 배지에 인간각질형성세포를 24 well 세포 배양 접시에 1×105 농도로 접종한 후 24시간 동안, 37℃, 5% CO2 습윤 조건에서 배양하였다. 이후, 배지를 제거하고 무혈청 DMEM 배지로 희석한 실시예 1에서 제조한 장미허브, 병풀, 티트리, 및 마치현 추출물을 농도별(10, 100, 및 250㎍/mL)로 처리하여 24시간 동안 배양하였다. 24시간 후, 1mg/mL의 MTT를 처리하고 2시간 후, 세포에 MTT 처리로 인해 생성된 포마잔(formazan)을 DMSO로 녹여 570nm에서 흡광도를 측정하였다.First, human keratinocytes were inoculated in a 24 well cell culture dish at a concentration of 1×10 5 in DMEM (Dulbeccos modified Eagles medium), a dedicated medium containing 10% FBS (fetal bovine serum, Cambrex), and then for 24 hours. , 37 ℃, 5% CO 2 was cultured in wet conditions. Thereafter, the medium was removed and the rose herb, centella asiatica, tea tree, and masquerade extract prepared in Example 1 diluted with serum-free DMEM medium were treated at different concentrations (10, 100, and 250 μg/mL) for 24 hours. Cultured. After 24 hours, 1 mg/mL of MTT was treated, and after 2 hours, formazan produced by the MTT treatment was dissolved in DMSO and absorbance was measured at 570 nm.
그 결과, 인간각질형성세포에 장미허브, 병풀, 티트리, 및 마치현 추출물을 처리한 경우, 각각의 추출물이 인간각질형성세포의 세포 생존율에 크게 영향을 미치지 않는 것으로 확인되었으며, 이를 통해 장미허브, 병풀, 티트리, 및 마치현 추출물이 각각 인체에 독성을 나타내지 않음을 확인할 수 있었다(도 1 참조).As a result, when the human keratinocytes were treated with rose herb, centella asiatica, tea tree, and masquerade extracts, it was confirmed that each extract did not significantly affect the cell survival rate of human keratinocytes. Centella asiatica, tea tree, and masquerade extracts were confirmed to not exhibit toxicity to the human body, respectively (see FIG. 1).
실시예Example 3. 히알루론산( 3. Hyaluronic acid ( HyaluronanHyaluronan ) 합성 촉진) Synthesis promotion
3-1. 단일 추출물의 효과3-1. The effect of a single extract
상기 실시예에 따라 제조된 장미허브, 병풀, 티트리, 및 마치현 추출물이 히알루론산(Hyaluronan) 합성에 미치는 영향을 실험하였다.The effect of the extracts of rose herb, centella asiatica, tea tree, and masquerade as prepared according to the above example was tested on the synthesis of hyaluronic acid (Hyaluronan).
또한, 히알루론산(HA, Hyaluronan, Hyaluronic acid)은 탄수화물 형태의 성분으로 진피층 및 표피 내에서 수분과 결합하여 세포가 정상적으로 활동할 수 있는 환경을 조성하는 중요한 역할을 하는 물질이다. 진피층 및 표피에 존재하는 생리활성 물질의 하나인 히알루론산은 섬유아세포에서 합성되는 물질로 히알루론산 합성효소(Hyaluronan synthase)에 의해 생산된다. 따라서, 본 실시예에서는 피부 보습과 직접적으로 관련된 인간각질형성세포 내에서 히알루론산 합성량을 확인하였다.In addition, hyaluronic acid (HA, Hyaluronan, Hyaluronic acid) is a substance in the form of carbohydrates and is a substance that plays an important role in creating an environment in which cells can function normally by binding with moisture in the dermis and epidermis. Hyaluronic acid, one of the physiologically active substances present in the dermis and epidermis, is a substance synthesized in fibroblasts and is produced by hyaluronic acid synthase (Hyaluronan synthase). Therefore, in this example, the amount of hyaluronic acid synthesis in human keratinocytes directly related to skin moisturization was confirmed.
인간각질형성세포를 24 well 세포배양접시에 1㎖의 배양액을 넣고 약 1×105의 농도로 세포를 접종한 후, 37℃, 5% CO2 환경에서 24시간 동안 배양하였다. 그 후, 실시예 1에서 제조한 장미허브, 병풀, 티트리, 및 마치현 추출물(10, 100, 및 250㎍/㎖)을 포함한 배지로 교체하여 24시간 배양하였다. 이후 배양액을 회수(harvest)하여 4℃, 7,500rpm 환경에서 5분간 원심분리(centrifuge)한 뒤, ELISA 방법을 이용하여 Hyaluronan(Hyaluronan kit, R&D Systems, Inc., Minneapolis, MN, USA)의 발현량을 확인하였다. Human keratinocytes were inoculated with 1 ml of culture medium in a 24 well cell culture dish, and the cells were inoculated at a concentration of about 1×10 5 , and then cultured for 24 hours in an environment of 37° C. and 5% CO 2 . Thereafter, it was cultured for 24 hours by replacing it with a medium containing rose herb, centella asiatica, tea tree, and machi-suki extract (10, 100, and 250 µg/ml) prepared in Example 1. Thereafter, the culture medium was harvested and centrifuged for 5 minutes in an environment at 4°C and 7,500 rpm, and then the expression level of Hyaluronan (Hyaluronan kit, R&D Systems, Inc., Minneapolis, MN, USA) using an ELISA method. Was confirmed.
히알루론산(HA, Hyaluronan, Hyaluronic acid)은 주로 표피 각질형성세포와 진피 섬유아세포에 의해 합성되며, 수분과 결합하여 세포가 정상적으로 활동할 수 있는 환경을 조성하는 중요한 역할을 하는 물질이다. 피부에서 히알루론산의 감소는 피부 탄력 저하 및 수분 함유량 감소의 직접적인 원인 중 하나이다. 따라서 본 실시예에서는 인간각질형성세포 내에서 히알루론산의 생성량을 확인하였다. 그 결과, 시료 무처리군(Nor)에 비해 실시예 1의 방법에 따라 제조된 장미허브, 병풀, 티트리, 및 마치현 추출물의 처리군에서는 히알루론산(Hyaluronan)의 생성량이 농도 의존적으로 증가하는 양상을 확인할 수 있었고, 이중 장미허브 추출물의 히알루론산(Hyaluronan) 생성 촉진능이 가장 우수한 것으로 확인되었다(도 2 참조). Hyaluronic acid (HA, Hyaluronan, Hyaluronic acid) is mainly synthesized by epidermal keratinocytes and dermal fibroblasts, and is a substance that plays an important role in creating an environment in which cells can function normally by binding with water. The reduction of hyaluronic acid in the skin is one of the direct causes of the decrease in skin elasticity and moisture content. Therefore, in this example, the amount of hyaluronic acid produced in human keratinocytes was confirmed. As a result, compared to the sample untreated group (Nor), the amount of hyaluronic acid (Hyaluronan) produced increases in a concentration-dependent manner in the group treated with the rose herb, centella asiatica, tea tree, and machisuki extract prepared according to the method of Example 1 Among them, it was confirmed that the rose herb extract had the best ability to promote hyaluronic acid production (see FIG. 2).
3-2. 복합 추출물의 효과3-2. Effect of complex extract
상기 실시예 3-1과 동일한 방법으로 히알루론산(Hyaluronan) 합성 변화를 측정하되, 장미허브, 병풀, 티트리, 및 마치현을 조합하여 이들의 복합 추출물의 효과를 검증하였다. The change in the synthesis of hyaluronic acid (Hyaluronan) was measured in the same manner as in Example 3-1, but the effect of the complex extract thereof was verified by combining rose herb, centella asiatica, tea tree, and machis.
복합 추출물은 10, 100, 및 250㎍/㎖의 농도를 사용하였고, 사용된 식물추출물 간의 중랑비는 하기 [표 1]에 나타내었다.Complex extracts were used at concentrations of 10, 100, and 250 ㎍ / ㎖, and the middle ratio between the plant extracts used are shown in Table 1 below.
[표 1][Table 1]
실험 결과, 도 3 및 도 4에 도시된 바와 같이, 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물은 전반적으로 높은 히알루론산(Hyaluronan) 생성 촉진능을 보이는 것으로 나타났다. 특히, 장미허브, 병풀, 티트리, 및 마치현이 5 내지 10:0.5-2:0.5-2:0.5-2의 비율로 혼합된 실험예 3 내지 8의 복합 추출물에서 우수한 히알루론산(Hyaluronan) 생성 촉진능이 관찰되었고, 장미허브, 병풀, 티트리, 및 마치현이 10:1:1:1의 비율로 혼합된 실험예 4의 복합 추출물에서 가장 우수한 효과가 관찰되었다. As a result of the experiment, as shown in FIGS. 3 and 4, it was found that the complex extracts of rose herb, centella asiatica, tea tree, and machi-hyeon showed high overall hyaluronic acid (Hyaluronan) production promoting ability. In particular, in the complex extracts of Experimental Examples 3 to 8 in which rose herb, centella asiatica, tea tree, and machi-hyun were mixed in a ratio of 5 to 10:0.5-2:0.5-2:0.5-2, excellent hyaluronic acid (Hyaluronan) production was promoted The ability was observed, and the most excellent effect was observed in the complex extract of Experimental Example 4 in which rose herb, centella asiatica, tea tree, and machi-hyeon were mixed in a ratio of 10:1:1:1.
즉, 각각의 추출물을 따로 사용하였을 때보다 본 발명에서와 같이 장미허브, 병풀, 티트리, 및 마치현 추출물을 일정한 비율로 혼합하여 사용하였을 때 상승적인 히알루론산(Hyaluronan) 생성 촉진 효과(synergy effect)를 보이는 것을 확인하였다.That is, the synergy effect of synergistic hyaluronic acid production when using a mixture of rose herb, centella asiatica, tea tree, and masquerade as in the present invention rather than using each extract separately. It was confirmed that it showed.
실시예Example 4. 4. FilaggrinFilaggrin 및 And HyaluronanHyaluronan synthasesynthase -3 유전자 발현 분석-3 gene expression analysis
상기 실시예 3에서 히알루론산(Hyaluronan) 생성 촉진 효과가 가장 우수한 것으로 나타난 실험예 4의 복합 추출물이 필라그린(Filaggrin, FLG, 각질층 형성 인자) 및 Hyaluronan synthase-3(HAS-3, 피부보습 인자) 유전자 발현에 미치는 영향을 실험하였다.The composite extract of Experimental Example 4, which was shown to have the best hyaluronic acid production promoting effect in Example 3, was Filaggrin (FLG, stratum corneum forming factor) and Hyaluronan synthase-3 (HAS-3, skin moisturizing factor). The effect on gene expression was tested.
먼저, 100mm 세포 배양 접시에 인간각질형성세포를 10% FBS를 첨가한 DMEM 배지에 1×106 세포 농도로 접종하여 37℃, 5% CO2 배양기에서 24시간 동안 배양하였다. 24시간 배양하여 상기 실험예 4의 추출물을 각각 100μg/mL 및 250μg/mL 농도가 되도록 DMEM 배지에 희석하여 제조한 희석용액을 첨가하여 24시간 동안 동일 조건에서 배양하였다. 이후 Trizol reagent(invitrogen, USA)를 이용하여 세포를 회수하여 RNA를 분리하였다. RT-PCR 방법을 이용하여 Filaggrin 및 hyaluronan synthase-3의 mRNA 발현량을 확인하였다. 사용한 프라이머는 다음과 같다:First, human keratinocytes were inoculated at a concentration of 1×10 6 cells in DMEM medium to which 10% FBS was added to a 100 mm cell culture dish, and cultured in a 37° C., 5% CO 2 incubator for 24 hours. After culturing for 24 hours, the extract of Experimental Example 4 was diluted in DMEM medium to a concentration of 100 μg/mL and 250 μg/mL, respectively, and a diluted solution prepared was added, followed by incubation under the same conditions for 24 hours. Then, RNA was isolated by recovering cells using a Trizol reagent (invitrogen, USA). The mRNA expression levels of Filaggrin and hyaluronan synthase-3 were confirmed using RT-PCR method. The primers used were as follows:
- Filaggrin : 정방향 5′-AAG CTT CAT GGT GAT GCG AC-3′, 역방향 5′-TCA AGC AGA AGA GGA AGG CA-3′-Filaggrin:
- Hyaluronan synthase-3: 정방향 5′-CCC AGC CAG ATT TGT TGA TG-3′, 역방향 5′-AGT GGT CAC GGG TTT CTT CC-3′-Hyaluronan synthase-3: forward 5′-CCC AGC CAG ATT TGT TGA TG-3′, reverse 5′-AGT GGT CAC GGG TTT CTT CC-3′
- GAPDH: 정방향 5'-ACC ACA GTC CAT GCC ATC AC-3', 역방향 5'-CCA CCA CCC TGT TGC TGT AG-3'.-GAPDH: Forward 5'-ACC ACA GTC CAT GCC ATC AC-3', Reverse 5'-CCA CCA CCC TGT TGC TGT AG-3'.
상기와 같은 프라이머를 이용하여 RT-PCR 수행하고, 그 결과를 도 5 및 도 6에 정리하였다.RT-PCR was performed using the above primers, and the results are summarized in FIGS. 5 and 6.
필라그린은 표피 과립층에서 케라토히알린과립(keratohyalin granule)을 형성하는 단백질인 프로필라그린(profilaggrin)의 형태로 존재하다가, 각질형성세포의 최종분화과정에서 필라그린으로 분해된 후에 각질세포막을 형성할 때 케라틴 필라멘트를 응집하여 각질세포의 단단하고 편평한 구조를 만듦으로써 피부장벽에서 벽돌(brick)의 역할을 수행하게 된다. 또한, 진피층 및 표피에 존재하는 생리활성 물질의 하나인 히알루론산은 섬유아세포에서 합성되는 물질로 히알루론산 합성효소(Hyaluronan synthase)에 의해 생산된다. Filaggrin exists in the form of profilaggrin, a protein that forms keratohyalin granules in the granular layer of the epidermis, and is decomposed into filaggrin in the final differentiation process of keratinocytes to form a keratinocyte membrane. At this time, keratin filaments are aggregated to form a hard and flat structure of keratinocytes, thereby playing the role of a brick in the skin barrier. In addition, hyaluronic acid, one of the physiologically active substances present in the dermis and epidermis, is a substance synthesized in fibroblasts and is produced by hyaluronic acid synthase (Hyaluronan synthase).
따라서, 본 실시예에서는 피부 보습과 직접적으로 관련된 인간각질형성세포 내에서 필라그린 유전자과 히알루론산 합성효소의 mRNA 발현을 확인하였다. 그 결과, 시료 무처리군에 비해, 장미허브, 병풀, 티트리, 및 마치현 추출물이 10:1:1:1의 비율로 혼합된 실험예 4의 복합 추출물 처리군에서는 필라그린(filaggrin) 유전자의 mRNA 발현량이 각각 331%(100μg/mL) 및 368%(250μg/mL) 증가하였고, 히알루론산 합성효소(hyaluronan synthase-3)의 mRNA 발현량이 각각 221%(100μg/mL) 및 236%(250μg/mL) 증가한 것으로 관찰되었다(도 5 및 도 6 참조). Accordingly, in this example, mRNA expression of the pilagrin gene and hyaluronic acid synthase was confirmed in human keratinocytes directly related to skin moisturization. As a result, compared to the sample untreated group, in the complex extract-treated group of Experimental Example 4 in which rose herb, centella asiatica, tea tree, and masquerade extract were mixed in a ratio of 10:1:1:1, the filaggrin gene was The mRNA expression levels increased by 331% (100 μg/mL) and 368% (250 μg/mL), respectively, and the mRNA expression levels of hyaluronan synthase-3 were 221% (100 μg/mL) and 236% (250 μg/mL), respectively. mL) was observed to increase (see FIGS. 5 and 6).
또한, 장미허브 단독 추출물과 비교하여도, 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물이 10:1:1:1의 비율로 혼합된 실험예 4의 복합 추출물 처리군에서는 필라그린(filaggrin)의 mRNA 발현량이 각각 175%(100μg/mL) 및 178%(250μg/mL) 증가하였고, 히알루론산 합성효소(hyaluronan synthase-3)의 mRNA 발현량이 각각 139%(100μg/mL) 및 141%(250μg/mL) 증가한 것으로 관찰되었다(도 5 및 도 6 참조). 상기와 같은 결과를 통해, 본 발명의 장미허브 단일 추출물, 또는 장미허브, 병풀, 티트리, 및 마치현의 복합 추출물을 포함하는 피부 보습용 조성물은 세포독성이 없고, 각질형성 및 히알루론산의 합성을 증가하여 피부 보습에 개선 효과가 있음을 확인할 수 있었으며, 특히, 장미허브, 병풀, 티트리, 및 마치현이 10:1:1:1의 비율로 혼합된 복합 추출물에서 피부 보습에 가장 높은 시너지 효과가 있음을 확인할 수 있었다.In addition, compared to the rose herb extract alone, the complex extract treatment group of Experimental Example 4 in which the complex extracts of rose herb, centella asiatica, tea tree, and masquerade were mixed in a ratio of 10:1:1:1, filaggrin ) MRNA expression levels increased by 175% (100 μg/mL) and 178% (250 μg/mL), respectively, and the mRNA expression levels of hyaluronan synthase-3 were 139% (100 μg/mL) and 141% ( 250 μg/mL) was observed to increase (see FIGS. 5 and 6). Through the results as described above, the composition for moisturizing skin comprising a single extract of rose herb of the present invention, or a complex extract of rose herb, centella asiatica, tea tree, and masquerade, has no cytotoxicity, and keratin formation and synthesis of hyaluronic acid It was confirmed that there was an improvement effect on skin moisturizing by increasing it. In particular, the complex extract in which rose herb, centella asiatica, tea tree, and masquerade were mixed in a ratio of 10:1:1:1 showed the highest synergistic effect on skin moisturizing. It could be confirmed that there is.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다.As described above, specific parts of the present invention have been described in detail, and it is obvious that these specific techniques are only preferred embodiments and are not intended to limit the scope of the present invention to those of ordinary skill in the art.
따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.Therefore, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.
Claims (8)
상기 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물은 10:1:1:1의 중량비인 것을 특징으로 하는,
피부 보습용 화장료 조성물.Including a complex extract of rose herb, centella asiatica, tea tree, and macula as an active ingredient,
The rose herb extract, centella asiatica extract, tea tree extract, and masquerade extract are characterized in that the weight ratio of 10:1:1:1,
Cosmetic composition for moisturizing the skin.
상기 조성물은 히알루론산(Hyaluronic acid)의 합성을 촉진하고, 필라그린(Filaggrin) 및 히알루론산 합성효소(HAS-3, hyaluronan synthase-3) 유전자의 발현을 증가시키는 것을 특징으로 하는 피부 보습용 화장료 조성물.The method of claim 2,
The composition promotes the synthesis of hyaluronic acid, and increases the expression of filaggrin and hyaluronan synthase-3 (HAS-3) genes. .
상기 조성물은 용액, 외용연고, 크림, 폼, 영양화장수, 유연화장수, 마스크 팩, 유연수, 유액, 메이크업베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린크림, 선오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 패치 및 스프레이로 구성된 군으로부터 선택되는 제형을 갖는 것을 특징으로 하는 피부 보습용 화장료 조성물.The method of claim 2,
The composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, mask pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion, A cosmetic composition for moisturizing skin, characterized in that it has a formulation selected from the group consisting of paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray.
상기 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물은 10:1:1:1의 중량비인 것을 특징으로 하는,
피부 보습용 식품 조성물.Including a complex extract of rose herb, centella asiatica, tea tree, and macula as an active ingredient,
The rose herb extract, centella asiatica extract, tea tree extract, and masquerade extract are characterized in that the weight ratio of 10:1:1:1,
Food composition for moisturizing the skin.
상기 장미허브 추출물, 병풀 추출물, 티트리 추출물, 및 마치현 추출물은 10:1:1:1의 중량비인 것을 특징으로 하는,
피부 보습용 의약외품 조성물.Including a complex extract of rose herb, centella asiatica, tea tree, and macula as an active ingredient,
The rose herb extract, centella asiatica extract, tea tree extract, and masquerade extract are characterized in that the weight ratio of 10:1:1:1,
A quasi-drug composition for skin moisturizing.
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