KR102311011B1 - A composition for promoting the proliferation of human adipose derived stem cell - Google Patents

Abstract

The present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone ), glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioctic acid, lactoflavin, xanthophyll ), onion, rice bran, bamboo, carrot and hibiscus extract or a cosmetic composition for improving skin elasticity and wrinkle improvement comprising one or more substances selected from the group consisting of extracts or fractions thereof as an active ingredient, food composition, pharmaceutical composition, quasi-drug composition , A composition for promoting proliferation of human adipose-derived stem cells, and a method for increasing skin elasticity, comprising administering the composition to an individual other than a human.
The composition of the present invention can be used for anti-aging purposes by enhancing the subcutaneous fat layer structure, increasing skin elasticity, and improving wrinkles by promoting the proliferation of human adipose-derived stem cells.

Description

Composition for promoting the proliferation of human adipose stem cells {A composition for promoting the proliferation of human adipose derived stem cell}

The present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone ), glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioctic acid, lactoflavin, xanthophyll ), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and at least one material selected from the group consisting of fractions of the extract as an active ingredient, a cosmetic composition for increasing skin elasticity and improving wrinkles, food composition , a pharmaceutical composition, a quasi-drug composition, a composition for promoting the proliferation of human adipose-derived stem cells, and a method of increasing skin elasticity, comprising administering the composition to a subject other than a human.

Adipose tissue, along with muscles, plays a role as a support for the structure of the skin. Although the number of fat cells is 30-40%, they are structurally important cells in the skin, accounting for 90% of the volume. It is known that the subcutaneous adipose tissue decreases with the aging of the individual, and accordingly, the elasticity of the skin decreases and wrinkles occur. In addition, there are many cases in which facial elasticity is reduced and wrinkles are increased when fat is reduced due to a rapid diet. Therefore, increasing the volume of the skin by controlling the number of adipose tissue that plays an important role in maintaining the structure in the skin can be an improvement method for skin elasticity and wrinkles. However, in the case of fat grafting surgery currently used to improve skin elasticity, the transplanted stem cells have a very low engraftment rate in vivo, and there are problems such as reoperation after a certain period of time and the transplanted fat clumping. Therefore, it is essential to study a material that can strengthen the structure of the subcutaneous fat layer without such surgery.

However, in the case of the conventional technique of directly injecting adipose-derived stem cells, safety problems may occur, and in the case of the technique of mimicking the components secreted in the cell culture process and supplying them to the skin, there is a limitation in that it is difficult to imitate the actual components as they are. In addition, human adipose-derived stem cells decrease the cell activity as the skin ages and the time taken for proliferation more than doubles (Gerontolog 2011; 57:66-75), so the adipose stem cells present in the aging skin has a problem in that the reactivity to other application technologies is greatly reduced. Therefore, it is essential to study a substance that can activate the subcutaneous adipose tissue by promoting the proliferation of adipose stem cells without skin irritation.

Accordingly, the inventors of the present invention, as a result of earnest efforts to find a substance that effectively promotes the proliferation of adipose-derived stem cells, raspberry ketone, gallic acid, caffeic acid, carnosic acid) , terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione menadione), thioctic acid, lactoflavin, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and fractions of the extracts are the proliferation of human adipose-derived stem cells. The present invention was completed by confirming that the material can be used for the purpose of increasing skin elasticity and improving wrinkles.

One object of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, Tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioctic acid, lactoflavin, Xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and one or more substances selected from the group consisting of fractions of the extract as an active ingredient, Skin elasticity increase and wrinkle improvement cosmetic to provide a composition.

Another object of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoful It is to provide a food composition for increasing skin elasticity and improving wrinkles, comprising one or more substances selected from the group consisting of rabine, xanthophyll, onion, rice bran, bamboo, carrot and hibiscus extract or fractions thereof as an active ingredient.

Another object of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lac Toflavin, xanthophylls, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and one or more substances selected from the group consisting of hibiscus extracts and fractions of the extract as an active ingredient, including as an active ingredient, a medicament for increasing skin elasticity and improving wrinkles to provide a composition.

Another object of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lac Topflavin, xanthophylls, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and quasi-drugs for improving skin elasticity and wrinkle improvement comprising at least one substance selected from the group consisting of fractions of the extract as an active ingredient to provide a composition.

Another object of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lac Toflavin, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and one or more substances selected from the group consisting of hibiscus extracts and fractions of the extract as an active ingredient, Human adipose-derived stem cell proliferation promotion To provide a composition for

Another object of the present invention is to provide a method for increasing skin elasticity, comprising administering the composition to a subject other than humans.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present application may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this application fall within the scope of this application. In addition, it cannot be seen that the scope of the present application is limited by the detailed description described below.

In one aspect for achieving the above object, the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol (terpineol), sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetinic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), thioctic acid, Lactoflavin (lactoflavin), xanthophyll (xanthophyll), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and one or more substances selected from the group consisting of each fraction of the extract as an active ingredient, Provided is a cosmetic composition for increasing skin elasticity and improving wrinkles. The composition comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 of the above substances. Dogs, 16, 17, 18, 19, 20, 21, 22, 23 or 24 substances may be included as active ingredients.

In addition, the present invention is raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin or It provides a cosmetic composition for increasing skin elasticity and improving wrinkles, comprising a cosmetically acceptable salt of Topil as an active ingredient.

In the present invention, "cosmetically acceptable salts" are raspberry ketone, gallic acid, caffeic acid, carnoic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione. , thioxane, lactoflavin, or xanthophyll is in the form of a salt combined with other substances, and refers to a substance that can cosmetically exhibit similar activity to the above compound.

The inventors of the present invention made diligent efforts to develop substances that can activate and restore the aged subcutaneous fat layer, and as a result, raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid , It was confirmed that lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, xanthophylls, onion extract, rice bran extract, bamboo extract, carrot extract and hibiscus extract promote the proliferation of human adipose-derived stem cells. Through this, it was found that the composition containing the material can be used as a cosmetic composition for increasing skin elasticity and improving wrinkles. The effect of increasing skin elasticity and improving wrinkles of the material is not known at all until now, and it can be said that it is very significant in that it was first developed by the present inventors.

The molecular formula of the term “Raspberry ketone” of the present invention is C ₁₀ H ₁₂ O ₂ , and the molecular weight is 164.20, which can be represented by Chemical Formula 1 below.

[Formula 1]

The raspberry ketone of the present invention is not limited to a method for obtaining the raspberry ketone, and a commercially available material may be used or chemically synthesized by a method known in the art.

The molecular formula of the term “gallic acid” of the present invention is C ₇ H ₆ O ₅ , and the molecular weight is 170.12, which can be represented by Formula 2 below.

[Formula 2]

Gallic acid of the present invention is not limited to a method for obtaining the gallic acid, and may be chemically synthesized by a method known in the art, or a commercially available material may be used.

The molecular formula of the term “caffeic acid” of the present invention is C ₉ H ₈ O ₄ , and the molecular weight is 180.157, which can be represented by Chemical Formula 3 below.

[Formula 3]

The caffeic acid of the present invention is not limited to a method for obtaining the caffeic acid, and a commercially available material may be used or chemically synthesized by a method known in the art.

The molecular formula of the term “carnosic acid” of the present invention is C ₂₀ H ₂₈ O ₄ , and the molecular weight is 332.44, which can be represented by Chemical Formula 4 below.

[Formula 4]

Carnosic acid of the present invention is not limited to the method for obtaining it, and may be chemically synthesized by a method known in the art, or a commercially available material may be used.

The molecular formula of the term “terpineol” of the present invention is C ₁₀ H ₁₈ O, and the molecular weight is 154, which can be represented by Chemical Formula 5 below.

[Formula 5]

The terpineol of the present invention is not limited to the method for obtaining it, and a commercially available material may be used or chemically synthesized by a method known in the art.

The molecular formula of the term "sodium mannose phosphate" of the present invention is C ₆ H ₁₂ O ₉ PNA, and the molecular weight is 282.12. Specifically, the sodium mannose phosphate may be sodium mannose 6-phosphate, or D-mannose-6-phosphate sodium salt, but is not limited thereto, which is represented by Formula 6 below. can

[Formula 6]

The sodium mannose phosphate of the present invention is not limited to a method for obtaining the sodium mannose phosphate, and may be chemically synthesized by a method known in the art, or a commercially available material may be used.

The molecular formula of the term “tropolone” of the present invention is C ₇ H ₆ O ₂ , and the molecular weight is 122, which can be represented by Chemical Formula 7 below.

[Formula 7]

Tropolon of the present invention is not limited to the method for obtaining it, and may be chemically synthesized by a method known in the art, or a commercially available material may be used.

The molecular formula of the term “glycyrrhetinic acid” of the present invention is C ₃₀ H ₄₆ O ₄ , and the molecular weight is 471, which can be represented by Chemical Formula 8 below.

[Formula 8]

The glycyrrhetic acid of the present invention is not limited to the method for obtaining it, and a commercially available material may be used or chemically synthesized by a method known in the art.

The molecular formula of the term “lanosterol” of the present invention is C ₃₀ H ₅₀ O, and the molecular weight is 427, which can be represented by Chemical Formula 9 below.

[Formula 9]

The lanosterol of the present invention is not limited to a method for obtaining the lanosterol, and a commercially available material may be used or chemically synthesized by a method known in the art.

The molecular formula of the term “sodium riboflavin phosphate” of the present invention is C ₁₇ H ₂₀ N ₄ NaO ₉ P, and the molecular weight is 478.33, which can be represented by the following Chemical Formula 10.

[Formula 10]

The sodium riboflavin phosphate of the present invention is not limited to a method for obtaining the sodium riboflavin phosphate, and a commercially available material may be used or chemically synthesized by a method known in the art.

The term “menadione” of the present invention is a yellow crystal that is a type of vitamin K, the molecular formula is C ₁₁ H ₈ O ₂ , and the molecular weight is 172.18, which can be represented by Chemical Formula 11 below.

[Formula 11]

Menadione of the present invention is not limited to the method for obtaining it, and may be chemically synthesized by a method known in the art, or a commercially available material may be used.

The term “thioctic acid” of the present invention is also referred to as lipoic acid, a molecular formula of C ₈ H ₁₄ O ₂ S ₂ , and a molecular weight of 206, which can be represented by Chemical Formula 12 below.

[Formula 12]

The thioxane or lipoic acid of the present invention is not limited to the method for obtaining it, and a commercially available material may be used or chemically synthesized by a method known in the art.

As used herein, the term “lactoflavin” refers to riboflavin isolated from milk (riboflavin, vitamin B ₂ ). The lactoflavin of the present invention has a molecular formula C ₁₇ H ₂₀ N ₄ O ₆ and a molecular weight of 376.36, which can be represented by the following Chemical Formula 13.

[Formula 13]

The lactoflavin of the present invention is not limited to a method for obtaining the lactoflavin, and a commercially available material may be used or chemically synthesized by a method known in the art.

The term "xanthophyll" of the present invention is a carotenoid oxycarotenoid pigment present in the green part of leaves, flowers, fruits, etc. of plants, and has a yellow color, molecular formula C ₄₀ H ₅₆ O ₂ , molecular weight is 569 . The xanthophyll may be specifically lutein, and may be represented by the following Chemical Formula 14.

[Formula 14]

The xanthophyll or lutein of the present invention is not limited to a method for obtaining the xanthophyll or lutein, and a commercially available material may be used or chemically synthesized by a method known in the art.

The raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin or xanthophyll are solvated It may exist in unsolvated form as well as in unsolvated form. In addition, the compound may exist in crystalline or amorphous form, and all such physical forms are included within the scope of the present invention.

In addition, it is apparent that the scope of the compounds includes not only the compounds, but also cosmetically, food, and pharmaceutically acceptable salts thereof.

The term “onion ( Allium cepa )” of the present invention is a biennial herb of the Liliaceae family, a monocotyledonous plant, and contains quercetin, chromium and allicin as main ingredients, and is known to have anti-inflammatory, blood sugar control, blood pressure lowering actions, etc. . The onion may be purchased commercially sold, or harvested or grown in nature without limitation, and roots, stems, leaves, etc. may be used without limitation.

As used herein, the term “rice bran” refers to a pulverized mixture of pericarp, seed hide, and aloe layer produced when milling brown rice to make polished rice. It is known to lower blood cholesterol, antioxidant, and anticancer effects.

As used herein, the term "bamboo ( Bambusoideae )" is an evergreen perennial plant belonging to the monocotyledonous plant, subfamily Bamboo, and known for its antioxidant, bactericidal, and anticancer effects in oriental medicine. The bamboo may be purchased commercially, or harvested or grown in nature without limitation, and roots, stems, leaves, etc. may be used without limitation.

As used herein, the term "carrot (carrot, Daucus carota var. sativa )" is a biennial herb of the dicotyledonous umbel family, and contains beta-carotene, lycopene, etc. is known The carrot may be purchased commercially sold, or harvested or grown in nature without limitation, and roots, stems, leaves, etc. may be used without limitation.

As used herein, the term "Hibiscus (Rose of China, Hibiscus )" refers to a plant belonging to the genus Mallow and Mugunghwa, a dicotyledonous plant, and is rich in hibiscus acid, hydroxycitric acid, etc. . The hibiscus may be purchased commercially, or harvested or grown in nature without limitation, and roots, stems, leaves, etc. may be used without limitation.

As used herein, the term "extract" refers to a liquid component obtained by immersing a target substance in various solvents and then extracting it for a certain period of time at room temperature, low temperature or heating condition, and removing the solvent from the liquid component. It means the result of solid content, etc. In addition, in addition to the result, it can be comprehensively interpreted as including all of the diluent of the result, the concentrate thereof, the prepared product thereof, the purified product, and the like.

In the present invention, the extract may be an onion, rice bran, bamboo, carrot or hibiscus extract. The extract may be extracted from various organs of natural, hybrid, and variegated plants, and may be extracted from, for example, roots, above-ground parts, stems, leaves, flowers, fruit bodies, fruit skins, as well as plant tissues. The extract can be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited as long as the extract can be obtained, but may be water, a polar solvent or a non-polar solvent, specifically water, a lower alcohol having 1 to 6 carbon atoms (methanol, ethanol, propanol or butanol), polyhydric alcohols such as glycerin and butylene glycol, hydrocarbon solvents such as methyl acetate and ethyl acetate, or mixed solvents thereof, or more specifically water, lower alcohol, 1,3-butylene glycol , or a mixed solvent thereof.

In addition, the method for obtaining the extract is not particularly limited thereto, as long as the extract can be obtained from the extract, but specifically, roots, stems, leaves, fruits, flowers, dried products thereof, Methods such as cold extraction, heating extraction, ultrasonic extraction by adding ultrasonic waves, and reflux extraction using a reflux condenser can be used by immersing the processed product in the solvent and extracting at room temperature.

As used herein, the term "fraction" refers to a result obtained by performing fractionation in order to separate a specific component or a specific group of components from a mixture including various components. In the present invention, the fraction may be a fraction of onion extract, rice bran extract, bamboo extract, carrot extract and hibiscus extract.

The fractionation method for obtaining the fraction in the present invention is not particularly limited thereto, as long as it can exhibit the effect of increasing skin elasticity and improving wrinkles, but may be performed according to a method commonly used in the art. For example, a solvent fractionation method performed by treating various solvents, an ultrafiltration fractionation method performed by passing through an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (separation according to size, charge, hydrophobicity or affinity) It can be a chromatographic fractionation method for performing a chromatographic fractionation method, and a combination thereof.

In the present invention, the type of the fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water, distilled water, and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. In the case of using alcohol in the fractionation solvent, preferably C1 to C4 alcohol may be used.

The cosmetic composition of the present invention includes raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin , xanthophyll, onion, rice bran, bamboo, carrot and hibiscus extract or one or more substances selected from the group consisting of fractions thereof, based on the total weight of the composition may include 0.0001 to 2% by weight (w / w), but this not limited If it is less than 0.0001% by weight, the effect may be insignificant, and if it is more than 2% by weight, a problem indicative of cytotoxicity may occur.

In the present invention, the cosmetic composition may promote the proliferation of human adipose derived stem cells. That is, the cosmetic composition may increase skin elasticity and improve wrinkles by promoting the proliferation of human adipose-derived stem cells.

As used herein, the term “human adipose derived stem cell” refers to a stem cell that can be harvested from human adipose tissue, and refers to a multipotent cell capable of differentiating into various cells of the body. Adipose-derived stem cells exist in subcutaneous adipose tissue and have the ability to differentiate into adipocytes. Adipokine secreted by adipose-derived stem cells is known to not only increase collagen synthesis in the dermal layer of the skin, but also affect skin regeneration.

As used herein, the term “adipose cell” refers to a cell that stores fat in adipose tissue of an individual, and specifically may refer to white adipocytes present in the subcutaneous fat layer, but is not limited thereto. does not

In one embodiment of the present invention, raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione in human adipose-derived stem cells , thioxan, lactoflavin, xanthophyll, onion, rice bran, bamboo, carrot and hibiscus extracts were treated to significantly increase the cell activity rate of human adipose-derived stem cells compared to the control group, thereby promoting their proliferation. It was confirmed that it is possible. Therefore, the composition of the present invention comprising the above material can be used for anti-aging purposes by increasing skin elasticity and improving wrinkles.

As used herein, the term “increasing skin elasticity” refers to enhancing skin elasticity by strengthening the subcutaneous fat layer structure of an individual using the composition of the present invention.

The term "improvement" of the present invention means alleviating the symptoms of skin wrinkles in an individual by using the composition of the present invention.

In the present invention, the cosmetic composition comprises a solution, an external ointment, a cream, a foam, a nourishing lotion, a softening lotion, a pack, a softening water, an emulsion, a makeup base, an essence, a soap, a liquid detergent, a bath agent, a sunscreen cream, a sun oil, Suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays may be prepared in a formulation selected from the group consisting of, but It is not limited.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and conventional ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures thereof may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component. In particular, in the case of a spray, additional chlorophyll propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty ester, fatty acid ester of polyethylene glycol or sorbitan may be used. have.

When the formulation of the present invention is a suspension, as a carrier component a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. may be used as carrier components. have.

The cosmetic composition of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactofle In addition to one or more substances selected from the group consisting of rabine, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and fractions of the extract, commonly used adjuvants such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic actives, preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, odorants and dyes, and the like.

In another aspect for achieving the above object, the present invention provides raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, mena Dione, thioxane, lactoflavin, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and one or more substances selected from the group consisting of hibiscus extracts as an active ingredient, skin elasticity It provides a food composition for increasing and improving wrinkles. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the proliferation of human adipose-derived stem cells. The raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, xanthophyll, onion, Rice bran, bamboo, carrot, hibiscus, extracts, fractions, skin elasticity increase and improvement are as described above.

In addition, the present invention is raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin or It provides a food composition for improving skin elasticity and improving skin elasticity, comprising a pharmaceutically acceptable salt of topil as an active ingredient.

In the present invention, "food pharmaceutically acceptable salts" are raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione. , thioxane, lactoflavin, or xanthophyll is in the form of a salt combined with other substances, and refers to a substance that can exhibit similar activity to the above compound in terms of food.

The food composition of the present invention is raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, In addition to one or more substances selected from the group consisting of xanthophylls, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and fractions of the extract, it may contain a food additive that is pharmaceutically acceptable.

In the present invention, "food supplementary additive" means a component that can be added auxiliary to food, it is added to manufacture a health functional food of each formulation, and those skilled in the art can appropriately select and use it. Examples of food supplement additives include various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like.

The functional food composition of the present invention may include a health functional food. In the present invention, "health functional food" refers to a food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. using raw materials or ingredients useful for the human body. Here, the term “functionality” refers to obtaining useful effects for health purposes such as regulating nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be manufactured by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and components commonly added in the art. In addition, if the formulation of the health functional food is also recognized as a health functional food, it may be manufactured without limitation. The food composition of the present invention can be prepared in various types of dosage forms, and unlike general drugs, it has the advantage of not having side effects that may occur during long-term administration of the drug using food as a raw material, and has excellent portability, Health functional food can be taken as a supplement to increase skin elasticity and improve wrinkles.

There is no limitation in the form that the health functional food of the present invention can take, and it may include any food in a conventional sense, and may be used interchangeably with terms known in the art, such as functional food. In addition, the health functional food of the present invention can be prepared by mixing known additives with other suitable auxiliary ingredients that may be included in the food according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and vitamin complex, etc. It also includes food used as feed for animals.

In another aspect for achieving the above object, the present invention provides raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, Menadion, thioxane, lactoflavin, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and skin comprising one or more substances selected from the group consisting of fractions of the extract as an active ingredient. It provides a pharmaceutical composition for increasing elasticity and improving wrinkles. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the proliferation of human adipose-derived stem cells. The raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, xanthophyll, onion, Rice bran, bamboo, carrot, hibiscus, extracts, fractions, skin elasticity increase and improvement are as described above.

In addition, the present invention is raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin or It provides a pharmaceutical composition for improving skin elasticity and improving skin elasticity, comprising a pharmaceutically acceptable salt of Topil as an active ingredient.

In the present invention, "pharmaceutically acceptable salts" are raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione. , thioxane, lactoflavin, or xanthophyll is in the form of a salt combined with another substance, and refers to a substance that can pharmaceutically exhibit similar activity to the above compound.

The pharmaceutical composition of the present invention can be used as a single formulation, and can be prepared and used as a combination formulation by additionally including a drug known to have an effect of increasing skin elasticity and improving wrinkles, and can be formulated using a pharmaceutically acceptable carrier or excipient. It may be prepared in unit dose form or by introduction into a multi-dose container.

The pharmaceutical composition may be administered in a pharmaceutically effective amount. The "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level depends on the subject type and severity, age, sex, activity of the drug, and the drug. Sensitivity to, administration time, administration route and excretion rate, duration of treatment, factors including concomitant drugs, and other factors well known in the medical field.

In another aspect for achieving the above object, the present invention provides raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, Menadion, thioxane, lactoflavin, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and skin comprising one or more substances selected from the group consisting of fractions of the extract as an active ingredient. To provide a quasi-drug composition for increasing elasticity and improving wrinkles. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes. The raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, xanthophyll, onion, Rice bran, bamboo, carrot, hibiscus, extracts, fractions, skin elasticity increase and improvement are as described above.

In addition, the present invention is raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin or It provides a quasi-drug composition for improving skin elasticity and improving skin elasticity, comprising a pharmaceutically acceptable salt of Topil as an active ingredient. The “pharmaceutically acceptable salt” is the same as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent if necessary in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it does not impair the effects of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. may include

The "pharmaceutically acceptable carrier" may mean a carrier, excipient or diluent that does not inhibit the biological activity and properties of the injected compound without irritating the organism, and specifically, non-naturally occurring carrier). The type of carrier usable in the present invention is not particularly limited, and any carrier commonly used in the art and pharmaceutically acceptable may be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and the like. These may be used alone or in mixture of two or more.

The composition comprising a pharmaceutically acceptable carrier may be in various oral or parenteral formulations. In the case of formulation, it is prepared using diluents or excipients, such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, It may be prepared by mixing gelatin or the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid formulations for oral use include suspensions, solutions, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, Witepsol, Macrogol, Tween 61, cacao butter, laurin, glycerogelatin, etc. may be used.

The quasi-drug composition of the present invention may include, but is not limited to, disinfectant cleaner, shower foam, ointment, wet tissue, coating agent, etc. It may be appropriately selected from the techniques of

In addition, the quasi-drug composition of the present invention may be used in a method for improving skin wrinkles, including applying to the skin of an individual. The subject includes, without limitation, mammals including rats, livestock, humans, and the like.

In another aspect for achieving the above object, the present invention provides raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, Menadion, thioxane, lactoflavin, xanthophyll, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and human fat comprising at least one substance selected from the group consisting of fractions of the extract as an active ingredient Provided is a composition for promoting proliferation of derived stem cells. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the proliferation of human adipose-derived stem cells.

The raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, xanthophyll, onion, Rice bran, bamboo, carrot, hibiscus, extracts, fractions, skin elasticity increase and improvement are as described above.

In another aspect for achieving the above object, the present invention provides a method of increasing skin elasticity, comprising administering the composition to an individual other than humans.

As used herein, the term "administration" means introducing the composition of the present invention to a patient by any suitable method, and the administration route of the composition may be administered through any general route as long as it can reach the target tissue. have. Intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, may be administered intranasally, but is not limited thereto. Since the composition of the present invention is effective in enhancing skin elasticity and improving wrinkles, the route of administration of the composition may be applied to the skin and administered.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into two to three times. In addition, the composition of the present invention may be used alone or in combination with other drug treatments for skin elasticity enhancement and wrinkle improvement. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, and can be easily determined by those skilled in the art.

As used herein, the term "individual" refers to all animals, including humans, rats, mice, livestock, etc. with reduced skin elasticity or with or with skin wrinkles. Specifically, it may be a mammal, including a human, but is not limited thereto.

The composition of the present invention can be used for anti-aging purposes by enhancing the structure of the subcutaneous fat layer, increasing skin elasticity, and improving wrinkles by promoting the proliferation of human adipose-derived stem cells.

1 is raspberry ketone, gallic acid, caffeic acid, carnous acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetic acid, lanosterol, sodium riboflavin phosphate, menadione, thioxane, lactoflavin, xanthophyll, This shows the cell activity rate in human adipose-derived stem cells treated with onion, rice bran, bamboo, carrot and hibiscus extracts.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Example 1: Preparation of onion, rice bran, bamboo, carrot and hibiscus extracts

In the present invention, the extracts of onion, rice bran, bamboo, carrot and hibiscus were respectively extracted using methanol, and then dried to form a powder.

Example 2: Confirmation of the proliferation-promoting effect of the compounds and extracts of the present invention on human-derived adipose stem cells

Human adipose derived stem cells were purchased from Lonza (Lonza, Inc. Walkersville, MD, USA) and then cultured in a carbon dioxide incubator at 37 degrees Celsius and 5% carbon dioxide conditions. After each material was treated at concentrations of 0.2 ppm, 2 ppm, and 20 ppm, the cell activity level of human adipose stem cells was confirmed using the CCK-8 kit (Dojindo). As a result, each material showed the highest activity at the concentration shown in Table 1 below, and all showed a cell activity rate that was 20% or more higher than that of the untreated group (control group) (FIG. 1).

[Table 1]

Therefore, a total of 19 compounds or extracts of the present invention significantly promote the proliferation of human adipose-derived stem cells, and can be used for anti-aging purposes by increasing skin elasticity and improving wrinkles.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims described below and their equivalents.

Claims (14)

One selected from the group consisting of Raspberry ketone, caffeic acid, glycyrrhetinic acid, menadione, thioctic acid and lactoflavin A cosmetic composition for increasing skin elasticity and improving wrinkles, comprising the above as an active ingredient.
The cosmetic composition of claim 1, comprising a cosmetically acceptable salt of raspberry ketone, caffeic acid, glycyrrhetic acid, menadione, thioxane or lactoflavin as an active ingredient.
The cosmetic composition according to claim 1, wherein the active ingredient is included in an amount of 0.0001 to 2% by weight (w/w) based on the total weight of the composition.
delete The cosmetic composition according to claim 1, wherein the active ingredient promotes the proliferation of human adipose derived stem cells.
According to claim 1, wherein the composition is a softening lotion (nutrient emulsion), astringent lotion, nutrient lotion, nutrition cream, massage cream, essence, pack, skin adhesion patch, skin adhesion gel, powder, ointment, suspension, emulsion, A cosmetic composition that is a formulation selected from a spray or a cosmetic solution.
One selected from the group consisting of Raspberry ketone, caffeic acid, glycyrrhetinic acid, menadione, thioctic acid and lactoflavin A food composition for increasing skin elasticity and improving wrinkles, comprising the above as an active ingredient.
According to claim 7, raspberry ketone, caffeic acid, glycyrrhetinic acid, menadione, thioxane, or a food composition comprising a pharmaceutically acceptable salt of lactoflavin as an active ingredient.
delete delete One selected from the group consisting of Raspberry ketone, caffeic acid, glycyrrhetinic acid, menadione, thioctic acid and lactoflavin A quasi-drug composition for increasing skin elasticity and improving wrinkles, comprising the above as an active ingredient.
The quasi-drug composition according to claim 11, comprising a pharmaceutically acceptable salt of raspberry ketone, caffeic acid, glycyrrhetinic acid, menadione, thioxane or lactoflavin as an active ingredient.
Raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, gly glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, thioctic acid, lactoflavin, xanthophyll, onion An extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and a composition for promoting the proliferation of human adipose-derived stem cells comprising at least one selected from the group consisting of fractions of the extract as an active ingredient.
One selected from the group consisting of Raspberry ketone, caffeic acid, glycyrrhetinic acid, menadione, thioctic acid and lactoflavin A method of increasing skin elasticity, comprising administering a composition comprising the above as an active ingredient to a subject other than a human.

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