KR101712608B1 - - Composition for comprising -terpineol for moisturizing and improving skin wrinkle - Google Patents

Abstract

Provided are a cosmetic composition for moisturizing skin, reducing skin wrinkles, inhibiting skin red spots, and promoting skin elasticity comprising -terpineol as an active ingredient, and a skin external agent. The composition comprising -terpineol is effective in increasing a secretion rate of procollagen, inhibiting red spots, promoting collagen biosynthesis, reducing expression of MMP-1, and inhibiting hypertrophy of the outer skin layer, thereby being effective in moisturizing skin, reducing skin wrinkles, inhibiting skin red spots, and promoting skin elasticity.

Description

TECHNICAL FIELD The present invention relates to a composition for improving skin moisturizing and skin wrinkles containing α-terpineol as an active ingredient,

The present invention relates to a composition for improving skin moisturizing and skin wrinkles containing α-terpineol as an active ingredient, and more particularly, to a composition for improving skin moisturizing and skin wrinkles containing α-terpineol as an active ingredient, And a cosmetic composition for enhancing skin elasticity, an external preparation for skin, a food composition and a pharmaceutical composition.

The skin consists largely of epidermis, dermis, and hypodermis. The epidermis, especially the stratum corneum, which is the outermost layer of the epidermis, acts as a skin barrier to inhibit the loss of moisture and electrolytes from the skin. While the dermis, through the synthesis of collagen and elastin, maintains the elasticity of the skin and supports the structure. In other words, collagen and elastin are the main proteins produced in fibroblasts, which are involved in the mechanical durability of the skin, the binding force and elasticity of the tissue. Collagen forms a variety of isoforms depending on its morphology and structural characteristics. There are 28 types of collagen isotypes in human tissues, including collagen types 1, 3. 6. 7. 13, 14, 17 are known. Collagen types 1 and 3 form the epidermal component of the dermis, while collagen type 7 is the main constituent of the dermal and epidermal junctions.

In skin connective tissue, type 1 collagen is the most abundant of extracellular matrix proteins, and other proteins such as elastin, fibronectin, integrin, fibrin, and proteoglycans exist. The newly synthesized procollagen is hydroxylated at the proline and lysine amino acid residues, and the three strands are twisted into the extracellular space. Here, procollagen is formed by collagen proteinase at the both ends by procollagen proteinase. The latter forms a microfibril of triple helix configuration. Microfibrils combine with leucine-rich small proteoglycans to form fibril . As a result, these fibrils are gathered together to form collagen fibers that provide the binding force and elasticity of the skin.

Skin aging is known to be caused by a decrease in collagen content, which is the protein that occupies most of the collagen in skin dermal tissue. Since collagen gives skin tension and strength, collagen reduction has a very deep relationship with skin aging and wrinkle formation have. Skin aging is largely divided into endogenous aging by physiological aging and photoaging caused by continuous exposure to ultraviolet radiation (UV). Repeated exposure to ultraviolet light results in increased collagenase and denaturation and destruction of collagen fibers, reducing skin elasticity and promoting the production of wrinkles.

Recent advances in medical technology have led to increased interest in skin care and health as life expectancy has been extended and quality of life has been improved and the desire for a healthy and beautiful life has increased. Various cosmetic functional cosmetics have been developed to maintain healthy skin. Especially, researches for prevention of skin wrinkle formation, mitigation and improvement have been actively carried out. In addition, recently, cosmetic ingredients reach skin dermis and there is a limitation in supplying nutrients. As a result of the change in perception that ingestion of nutrients or functional ingredients to the skin can provide a skin cosmetic enhancement effect, Research to excavate materials is also being actively pursued.

Thus, the present applicant has conducted research to develop a food or cosmetic material having an effect on moisturizing and wrinkle reduction by suppressing the action of collagenase in natural products with low side effects and promoting the synthesis of collagen.

Accordingly, the present inventors confirmed that? -Terpineol has skin moisturizing effect, suppression of erythema and alleviation of skin wrinkles, and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition, an external preparation for skin and a food composition for skin moisturization, skin wrinkle suppression, erythema suppression and skin elasticity enhancement containing? -Terpineol as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for skin moisturizing and wrinkle improvement, which contains? -Terpineol as an active ingredient.

According to another preferred embodiment of the present invention,? -Terpineol may be contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic.

According to another preferred embodiment of the present invention, the composition of the present invention is effective to any one or more of effects selected from the group consisting of an increase in procollagen secretion amount, promotion of collagen biosynthesis, reduction of expression of MMP-1 gene and inhibition of skin stratum corneum formation It can be something to have.

According to another preferred embodiment of the present invention, the cosmetic composition of the present invention is used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, It can be any one selected from the group consisting of essence, pack, mask pack, mask sheet, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder and eye shadow have.

According to another preferred embodiment of the present invention, the composition of the present invention may further comprise a cosmetically acceptable carrier.

According to another preferred embodiment of the present invention, the composition of the present invention may further comprise a skin wrinkle improving component or a skin elasticity improving component. The skin wrinkle improving component or skin elasticity enhancing component may be specifically selected from the group consisting of vitamin C, retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract.

The present invention also provides a composition for external application for skin for moisturizing and wrinkling, which contains? -Terpineol as an active ingredient.

The present invention also provides a food composition for improving skin moisturization and skin wrinkles containing? -Terpineol as an active ingredient.

According to another preferred embodiment of the present invention, the food of the present invention may be made into any one form selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings.

The present invention also provides a pharmaceutical composition for improving skin moisturizing and wrinkles containing? -Terpineol as an active ingredient.

The present invention also provides a cosmetic composition for enhancing skin elasticity, an external preparation for skin, a food composition and a pharmaceutical composition containing? -Terpineol as an active ingredient.

The present invention also provides a composition for suppressing erythema comprising? -Terpineol as an active ingredient.

The present invention provides a cosmetic composition for skin moisturization, skin wrinkle suppression, skin erythema suppression, skin elasticity enhancement, and external preparation for skin containing α-terpineol as an active ingredient. The composition containing? -terpineol has an effect of increasing the amount of procollagen secretion, promoting collagen biosynthesis, decreasing expression of MMP-1 gene, and inhibiting skin skin layer thickening, and is effective for skin moisturization, skin wrinkle improvement or skin elasticity enhancement .

FIG. 1 is a graph showing the amount of procollagen secreted from human skin fibroblasts (-UVB: control group without UVB treatment, + UVB: control group treated with UVB,? TN: treated with? -Terpineol after UVB irradiation Experimental group, VitC: Control group treated with UVB and vitamin C treatment, αTN + VitC: Experimental group treated with α-terpineol and vitamin C after UVB irradiation).
2 is an electrophoresis photograph and a graph showing the amounts of procollagen and MMP-1 gene expression in human fibroblasts.
FIG. 3 is a graph showing weight gain (A and B) and dietary intake amounts (C and D) of a mouse consuming an experimental diet.
4 is a graph showing moisture content (A), moisture evaporation amount (B), elasticity (C), and erythema index (D) of mouse skin tissues consumed in the experimental diet.
Fig. 5 is a photograph of the dorsal skin tissue of a mouse ingested with an experimental diet.
FIG. 6 is a photograph (A) and a graph (B) showing the degree of wrinkling of the skin tissue such as a mouse ingested with the experimental diet.
Fig. 7 is a graph (A) and a photograph (B) showing the skin thickness of a mouse ingested with the experimental diet.
8 is an electrophoresis photograph and graph showing changes in collagen and MMPs gene expression in tissues such as mice fed with alpha -terpineol.

Hereinafter, the present invention will be described in more detail.

As described above,? -Terpineol has been known for the purpose of pain suppression, but no research has been conducted on skin moisturizing, erythema suppression, skin elasticity enhancement, and skin wrinkle relief.

The composition containing? -Terpineol provided by the present invention has an effect of increasing the amount of procollagen secretion, promoting collagen biosynthesis, decreasing expression of MMP-1 gene, and inhibiting skin skin layer thickening, It is effective for improving or improving skin elasticity.

Accordingly, the present invention provides a cosmetic composition for improving skin moisturization and skin wrinkles containing? -Terpineol as an active ingredient.

? -terpineol is a monoterpene compound having a Cas No. of 98-55-5, a structural formula of C ₁₀ H ₁₈ O and a molecular weight of 154.25 g / mol. The structure is shown in the following formula (1). α-terpineol is 3-cyclohexene-1-methanol; alpha, alpha-4-trimethyl; 1-p-menthen-8-ol; p-menth-1-en-8-ol (isomer unspecified); 1-methyl-4-isopropyl-1-cyclohexen-8-ol; alpha -terpilenol; alpha-terpineol; terpineol schlechthin and so on. α-Terpineol is colorless crystals with melting point of 31-35 ° C and boiling point of 217-218 ° C.

[Chemical Formula 1]

α-terpineol is separated from various natural products such as Acacia farnesiana (Cassia flowers), Achillea millefolium (Carpenter's Weed), Acorus calamus (Calamus), Aframomum melegueta (Alligator Pepper, Guinea ginger) Can be extracted.

α-terpineol has been registered as a flavoring agent in Korean KFDA and US FDA food additives database, and is classified as GRAS (generalized as safe as a flavor ingredient) in FEMA (Flavor and Extract Manfacturers' Association). 3045). It is mainly used as a fragrance ingredient of cosmetics, fragrance, fragrance, shampoo, soap and detergent, and 100-1000 tons per year is used worldwide.

It has been reported that the bioactivity of α-terpineol has been reported to have an effect of lowering blood pressure by expanding mesenteric blood vessels. Recently, it has been reported that α-terpineol reduces arterial blood pressure and improves antioxidant activity in hypertensive rats induced by N-nitro-L-arginine methyl ester. In a pain model of mice induced by Carrageenan, α-terpineol has been shown to relieve pain and improve pleural inflammation. However, the effect of moistening and improving wrinkles of alpha-terpineol is not known.

The LD ₅₀ value of α-terpineol was reported to be 5,170 mg / kg orally in rats and 2,830 mg / kg in mice and 2,000 mg / kg in intramuscular injections. In addition, α-terpineol did not show genetic mutation in the Ames test and did not show any carcinogenicity in lung cancer model mice.

As a study on the metabolism of α-terpineol, 100 mg of α-terpineol was intraperitoneally injected into rats in a metabolic experiment. As a result, the glucuronide conjugate of α-terpineol and p- menthol-1, 2, 8, -triol.

Therefore, administration of α-terpineol at a low concentration, specifically 0.001 to 5170 mg / kg, more preferably 0.001 to 2000 mg / kg, is toxic or insignificant, so that the cosmetic composition, external preparation for skin, Can be used as a pharmaceutical composition.

As used herein, the term "improvement of skin wrinkles" means maintaining or enhancing the ability of skin to be associated with wrinkles and elasticity. Collagen (collagen) and elastin (elastin), which are the collagen fibers of the skin dermis layer, play a key role in maintaining skin elasticity. Collagen biosynthesis is affected by internal and external skin. Specifically, the skin cells are reduced in cellular activity due to natural aging, and collagen fibers are decreased. When extreme ultraviolet rays are irradiated as an external factor, or when active oxygen generated by stress or the like causes thiol (-SH) To increase the expression of collagen and elastase, thereby increasing the wrinkles of the skin and reducing the elasticity, thereby aging the skin.

The? -Terpineol contained in the cosmetic composition described in the present invention may be contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic. Less than 0.0001% by weight of? -Terpineol in cosmetics may not have a wrinkle-improving effect due to its small amount, and? -Terpineol of 20% by weight or more may exhibit the previously known toxicity.

The composition of the present invention may be one having at least one effect selected from the group consisting of an increase in procollagen secretion amount, promotion of collagen biosynthesis, reduction of expression of MMP-1 gene, and inhibition of skin stratum corneum formation.

According to one embodiment of the present invention, the amount of procollagen and procollagen type I C-peptide (PIP) secreted into the extracellular matrix by treating the drug with irradiation of ultraviolet rays to human skin fibroblasts was measured. As a result, As shown, the amount of procollagen secreted in UV-irradiated control cells (+ UVB) was significantly lower than that in normal cells (-UVB), and in the cells treated with α-terpineol with ultraviolet irradiation, Compared with cells (+ UVB), the amount of collagen was significantly increased by 27%. Compared with control cells (+ UVB), the amount of collagen was significantly increased by 54% in the cells treated with α-terpineol compared to the control cells (+ 27%) or α-terpineol (+ 27% ) Than the amount of collagen observed in cells treated alone (see Fig. 1).

According to one embodiment of the present invention, the expression of collagen type 1α1 and α2 and collagen type 3α1 was significantly increased in the α-terpineol-fed group compared to the + UVB-treated group, and the expression of MMP-1a and -1b, MMP- 3, and MMP-9 gene expression was significantly decreased (see FIG. 8). Therefore, α-terpineol can increase collagen protein synthesis in subcutaneous tissues and inhibit collagen fiber degradation, thereby alleviating wrinkle formation caused by ultraviolet irradiation.

According to one embodiment of the present invention, the changes in the expression of procollagen and MMP-1 gene after treatment of drugs with human skin fibroblasts were measured. As shown in FIG. 2, α-terpineol , While significant increases in MMP-1 gene expression were significantly reduced by ultraviolet light. The gene expression regulating activity of? -Terpineol was similar to that of vitamin C (see FIG. 2).

The term "cosmetic composition" used in the present invention is a composition containing the above-mentioned compound, and the formulation can be in any form. Examples of such formulations include cosmetic preparations made using the above composition, such as creams such as nutritional creams, eye creams, massage creams, cleansing creams, lotions such as packs, nutritional lotions, essences, softening longevity, A foundation, a makeup base, and the like, and may be manufactured and commercialized in any form of these formulations in order to achieve the object of the present invention, and the present invention is not limited to these examples. Further, the cosmetic composition according to the present invention can be formulated into a usual cosmetic preparation method.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, And may be any one selected from the group consisting of soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder and eye shadow.

The cosmetic composition of the present invention may further comprise a cosmetically acceptable carrier, and it is possible to apply the usual ingredients to be blended with the general skin cosmetic composition as needed.

Specifically, the cosmetic composition of the present invention may further comprise a transdermal penetration enhancer. As used herein, the term transdermal penetration enhancer is a composition that allows a desired component to penetrate into vascular cells of the skin at a high absorption rate. But are not limited to, other phospholipid components, liposomal components, and the like, preferably used in lecithin cosmetics.

As the oil which can be mainly used as an oil component, at least one selected from vegetable oil, mineral oil, silicone oil and synthetic oil can be used. More specifically, there may be mentioned mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, Vitisviniferase seed oil, macadamia nut oil, glyceryl octanoate, castor oil, ethylhexyl isononanoate, Chicone, cyclopentasiloxane, sunflower seed oil and the like can be used.

Further, 0.1 to 5% by weight of a surfactant, a higher alcohol and the like may be added to reinforce the emulsifying ability. As such surfactants, conventional surfactants such as nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, phospholipids and the like can be used. Specific examples thereof include sorbitan sesquiunorate, polysorbate 60 Sorbitan stearate, sorbitan stearate, sucrose stearate / sucrose cocoate, glyceryl stearate / polyethylene glycol-100 Stearate, cetearyl-6 olivate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside. Polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil, and the like. As the higher alcohol, an alcohol having 12 to 20 carbon atoms such as cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol, etc. may be used alone or in admixture of one or more.

In order to control the viscosity or hardness of the water phase, 0.001 to 5% by weight of at least one thickening agent such as carbomer, xanthan gum, bentonite, magnesium aluminum silicate, cellulose gum, dextrin palmitate and the like may be further added.

In addition, the cosmetic composition of the present invention may contain, if necessary, a medicinal ingredient such as higher fatty acids and vitamins, an ultraviolet screening agent, an antioxidant (butylhydroxyanisole, gallic acid propylate, eicosorbic acid, tocopheryl acetatide, (Triethanolamine, citric acid, citric acid, sodium citrate, malic acid, citric acid, etc.), preservatives (methylparaben, butylparaben, propylparaben, phenoxyethanol, imidazolidinyl urea, chlorphenesin and the like) And the like), a humectant (glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerin, etc.), sodium hydrogen carbonate , Betaine, glycereth-26, methylglucose-20, etc.), lubricant, and the like.

In addition, the cosmetic composition of the present invention further comprises a substance capable of supplementally providing an essential nutrient to the skin, and preferably it may contain an auxiliary agent including, but not limited to, a natural flavor, a cosmetic flavor, have.

In addition, the cosmetic composition of the present invention may further comprise a skin wrinkle improving component or a skin elasticity improving component. The specific skin wrinkle improving component or skin elasticity enhancing component may be at least one selected from the group consisting of vitamin C, retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, and most preferably, Vitamin C < / RTI >

In one embodiment of the present invention, when vitamin C and α-terpineol were treated together, the amount of procollagen was increased by 54%, but when vitamin C alone was treated, α-terpineol alone was treated by 27% 27% of the patients showed an increase in secretion of procollagen. When these results are assigned to call informality, it can be confirmed that a synergistic effect is obtained when α-terpineol and vitamin C are treated together.

On the other hand, a statistically effective weight loss effect was confirmed in the group to which α-terpineol was administered (FIG. 3), even though the same feed amount was administered to the experimental rats in the course of measuring the skin wrinkle improving effect through the experimental diet. Therefore,? -Terpineol of the present invention is characterized by further having a weight loss effect.

The present invention provides a composition for external application for skin for moisturizing skin and improving wrinkles of skin, which contains? -Terpineol as an active ingredient.

The composition for external application of the present invention is used for the purpose of maintaining skin moisturization, improving the wrinkles of the skin and preventing / delaying the generation of wrinkles, and is not particularly limited in its formulation. For example, It may be a cosmetic composition having a formulation of a lotion, a nutritional lotion, a massage cream, a nutritional cream, a pack, a mask pack, a mask sheet, a gel or a skin adhesive type cosmetic composition and may be a cosmetic composition such as a lotion, ointment, gel, cream, patch, It may be a transdermal dosage form.

Further, in the external preparation for each formulation, components other than the above-mentioned essential components can be mixed and selected without difficulty by those skilled in the art depending on the formulation or purpose of use of the other external preparation.

The present invention provides a skin-moisturizing and non-skin wrinkle-improving food composition containing? -Terpineol as an active ingredient.

The food composition of the present invention may be prepared in any one form selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings. The food composition according to the present invention can be formulated in the form of powders, liquids, tablets, soft capsules, granules, tea bags, instant tea or drinks containing α-terpineol as an active ingredient. The content of? -Terpineol as an active ingredient can be suitably determined according to the intended use (for prevention or improvement). Generally, the amount of? -Terpineol contained in the food composition may be 0.1 to 90% by weight based on the total weight of the food. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range. In addition, the food composition according to the present invention may contain other components, such as vitamin C, which may give a synergistic effect to the main effect, in addition to? -Terpineol, within the range not impairing the intended main effect of the present invention Or a natural substance such as green tea extract, mulberry extract, licorice extract, mulberry bark extract, bingo extract, golden extract, and wild ginseng extract.

The food composition formulated in this form can be added directly to the food or used together with other food or food ingredients, and can be suitably used according to conventional methods. Examples of food products include dairy products including drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, soups, , Dairy products and dairy products, and all health functional foods in the ordinary sense are included.

When the food composition of the present invention is a drink, it contains? -Terpineol as an essential ingredient at the indicated ratio. There are no particular restrictions on other ingredients used for the purpose of preparing other drink products, and various flavors Or natural carbohydrate as an additional ingredient. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors and synthetic flavors and the like can be used as the flavors other than those described above. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition, the food composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the inventive food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so important, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of? -Terpineol of the present invention.

The present invention provides a pharmaceutical composition for improving skin moisturization and skin wrinkles containing? -Terpineol as an active ingredient.

The compound of the present invention has an effect of inhibiting aging of cells due to inhibition of cell senescence by UV and formation of reactive oxygen species and promoting the expression of extracellular matrix proteins in skin cells, Or as a pharmaceutical composition for improving skin wrinkles.

The amount of the pharmaceutical composition for improving wrinkles or skin elasticity used in the present invention should be a pharmaceutically effective amount. As used herein, the term "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment and the effective dose level will vary depending on the species and severity, The type of virus being infected, the activity of the drug, the sensitivity to the drug, the time of administration, the route of administration and rate of release, the duration of the treatment, factors including co-administered drugs and other well known factors in the medical field. Effective amounts may vary depending on the route of treatment, the use of excipients, and the likelihood of use with other agents, as will be appreciated by those skilled in the art.

The pharmaceutical compositions for improving wrinkles or improving skin elasticity of the present invention may be formulated into pharmaceutical formulations using methods well known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal . In the preparation of the formulations, it is preferred that the active ingredient is mixed with or diluted with the carrier, or enclosed in a carrier in the form of a container.

Therefore, the pharmaceutical composition for wrinkle improvement or skin elasticity enhancement of the present invention can be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup or aerosol, May be formulated and used, and may further comprise suitable carriers, excipients or diluents conventionally used in the preparation of the composition.

Examples of carriers, excipients and diluents that can be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.

In addition, the pharmaceutical composition of the present invention can be added to the skin during moisturizing or improving the skin wrinkles. When the pharmaceutical composition of the present invention is used as a quasi-drug additive, the compound may be added as it is or may be used together with other quasi-drugs or quasi-drugs, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment).

The present invention also provides a cosmetic composition for enhancing skin elasticity, an external preparation for skin and a food composition containing? -Terpineol as an active ingredient.

In addition, the present invention provides a composition for suppressing erythema comprising alpha -terpineol as an active ingredient.

According to one embodiment of the present invention, the erythema index decreased by 28% in the group of mice administered with alpha -terpineol and decreased by 19% in the group administered with vitamin C. This means that it has a better erythema suppression effect than vitamin C.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

Effect of α-terpineol on the wrinkles of human skin fibroblasts

1-1) Cell culture

Normal human dermal fibroblasts (neonatal foreskin) were purchased from ATCC (Manassas, VA, USA). The cells were cultured in a 5% CO ₂ incubator at 37 ° C using a fibroblast growth medium (Promo Cell, Heidelberg).

1-2) Measurement of procollagen type I C-peptide (PIP) concentration

Collagen biosynthesis of human fibroblasts to investigate the performance of a 12-well from 1.0 × 10 ⁶ cells / well by frequency division by α- terpineol and vitamin C CO ₂ incubator for 24 hours to a concentration of 100 μM each of the plates Lt; / RTI > After removing the medium from each well, the plate was washed once with PBS, and 1 ml of PBS was added thereto. Then, ultraviolet B (UVB) was irradiated at 20 mJ / cm ² . The PBS of each well was again replaced with the medium and cultured for 24 hours. The amount of procollagen secreted in the medium was measured using a procollagen type I C-peptide EIA kit (Takara Bio, Japan). The standard solution contained in the collagen measurement kit was diluted by concentration, absorbance was measured at 450 nm to prepare a standard concentration curve and the amount of collagen production was calculated.

1-3) RNA isolation and RT-PCR using the triazole method (reverse transcription-polymerase chain reaction)

After adding 334 μl of trizol solution per 1 × 10 ⁷ cells of human skin fibroblasts, they were changed and centrifuged at 12,000 × g for 10 minutes at 4 ° C. The supernatant was transferred to a new tube, and 67 μl of chloroform was added and vortexed. The supernatant was again transferred to a new tube and isopropanol was added so that the ratio of supernatant to isopropanol was 1: 1. After 10 h of shaking, the mixture was allowed to stand at room temperature for 15 minutes, centrifuged at 12,000 × g for 10 minutes at 4 ° C., and then the supernatant was removed. 1 ml of 70% ethanol was added to the remaining precipitate, And centrifuged for 5 minutes. After removing the ethanol, the tube containing the RNA precipitate was dried at room temperature for 15 minutes, and the RNA pellet was dissolved using water containing no nucleic acid degrading enzyme. The concentration of RNA samples extracted at 260 nm and 280 nm wavelengths was measured using a UV / VIS spectrophotometer (Beckman coulter, DU730) and agarose gel electrophoresis was performed to confirm the integrity of the RNA samples Respectively.

CDNAs were synthesized by using oligo dT primers and superscript reverse transcriptase (GIBCO BRL, Gaithersburg, MD, USA) as a template for RNA samples extracted from human skin fibroblasts. PCR was carried out using the cDNA obtained from the reverse transcription as a template and using the 5 'and 3' flanking sequences of the gene cDNA to be amplified as primers, wherein the primer sequences used were as shown in [Table 1] Same as. 1 μl of the amplified PCR product was electrophoresed on 1% agarose gel to confirm the DNA band.

The primer sequence used for RT-PCR gene primer The base sequence (5 '- > 3') Annealing  Temperature
(° C) PCR
product
(bp) SEQ ID NO: Procollagen F TCTTCAAGCCATCCTGTGTG 60 168 One R GCGAGTCTGTGTTTTTGCAG 2 Metalloproteinase 1 (MMP-1) F ATGACATGAGTCCGGAGCAA 60 122 3 R TCATCTCCTGGGTCCCTTTC 4 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) F GTGATGGCATGGACTGTGGT 55 203 5 R GGAGCCAAAAGGGTCATCAT 6

1-4) Results of measurement of changes in the amount of procollagen secretion

Collagen, the main protein that makes up the skin, is synthesized in the form of procollagen in fibroblasts in the skin dermis and then secreted into the extracellular matrix. Procollagen secreted into the extracellular matrix is broken down by the pro-collagen peptidase present on the cell surface and is formed into active collagen. Therefore, the activated collagen content can be measured by measuring the C-peptide content. As shown in FIG. 1, the amounts of procollagen and procollagen type I C-peptide (PIP) secreted into the extracellular matrix were measured by irradiating ultraviolet light to human skin fibroblasts, Compared with control cells (+ UVB) irradiated with ultraviolet rays, cells treated with α-terpineol with ultraviolet irradiation showed a higher amount of collagen (+ UVB) than those of normal cells (-UVB) Was significantly increased by 18%. Compared with control cells (+ UVB), the amount of collagen was significantly increased by 54% in the cells treated with α-terpineol compared to the control cells (+ 27%) or α-terpineol (+ 27% ) Higher than the amount of collagen observed in cells treated alone (see Figure 1). Thus, α-terpineol increases the amount of collagen in human dermal fibroblasts, and this collagen-increasing effect appears to be more effective when used with vitamin C.

1-5) Measurement results of pro-collagen and MMP-1 gene expression changes

As a result of measuring the changes in the expression of procollagen and MMP-1 gene after treatment of the human skin fibroblasts with the drug, as shown in FIG. 2, α-terpineol significantly decreased the expression of procollagen significantly reduced by ultraviolet light While the expression of MMP-1 gene significantly increased by ultraviolet light was significantly reduced. The gene expression regulating activity of? -Terpineol was similar to that of vitamin C (see FIG. 2). Thus, α-terpineol may be involved in increasing collagen content, which is ultimately related to skin wrinkling, by inhibiting MMP-1 expression as well as increasing procollagen synthesis in human skin fibroblasts irradiated with ultraviolet light .

Evaluation of efficacy of moisturizing and elasticity enhancement of skin wrinkle of α-terpineol using mouse

2-1) Preparation of empirical formula, breeding of experimental animals and ultraviolet irradiation

The 5-week-old female albino hairless mouse (SKH-1) used in this experiment was purchased from Orient Bio (Korea) and subjected to a week of adaptation period with solid feed. The experimental animals were divided into 4 groups and 4 mice were assigned to each group. All experimental groups were divided into two groups: normal controls (-UVB), UV-irradiated group (UVB), α-terpineol (αTN) or vitamin C (VitC). During the breeding period, feed and water were freely consumed. The temperature was maintained at 22 ± 1 ° C, the humidity was maintained at 60 ± 5%, and the light period and the dark cycle were adjusted to 12 hours each day.

-UVB group and + UVB group received a purified diet prepared according to the AIN-93 mouse diet composition (Reeves, PG et al., J Nutr, 123: 1939-1951, 1993) The diet prepared by adding 0.2% α-terpineol (Sigma-Aldrich) to the purified diet and 0.2% vitamin C (Sigma-Aldrich) to the AIN-93 purified diet for the VitC group was fed for 13 weeks. The composition of the experimental diet is shown in [Table 2]. Diets were supplied daily with water between 10 am and 11 am, and the dietary intake was measured daily.

During the experimental period, UV rays were irradiated three times a week on the dorsal part of dull mice. The UV dose was 73 mJ / cm ² for the first week, 146 mJ / cm ^{2 for the second} week, 219 mJ / cm < ^{2 &} gt ;. During the breeding, weekly skin weight measurements and skin thickness measurements were taken. The skin thickness was measured using a digital micro caliper (Marathon Watch Company Ltd, Ontario, Canada) at the buttocks of the hairless mice. The calipers used in the measurement were able to measure up to 0.01 mm and had a control function to apply constant force to the thickness, and it was possible to measure the skin thickness under the same force.

After the end of the experiment, the animals were anesthetized and blood was collected and used for hematological analysis. The dorsal skin tissues were cut, some were stored in the freezer and used for molecular biology, and some were stored in 10% formalin solution Were used for immunohistochemical staining.

Experimentation table ingredient AIN-93G diet
(g / kg diet) alpha-terpineol Supplement diet (? TN)
(g / kg diet) Vitamin C supplement (VitC)
(g / kg diet) Casein 200 200 200 Maltodextrin 132 132 132 Corn starch 397.486 395.486 395.486 Sucrose 100 100 100 cellulose 50 50 50 Soybean oil 70 70 70 Vitamin complex 10 10 10 Mineral complex 35 35 35 Colin Beavertre 2.5 2.5 2.5 L-cystine 3 3 3 Tert-butyhydroquinone 0.014 0.014 0.014 alpha-terpineol - 2 - Vitamin C - - 2 Total (g) 1,000 1,000 1,000

2-2) Skin moisturizing, elasticity and erythema index measurement

The skin moisture content, water evaporation, elasticity and erythema index of the experimental animals were measured once at the end of the experiment using Corneometer ^® , Tewameter ^® , Cutometer ^® , and Mexameter ^® (CK Electronics GmbH). In the measurement, the numerical value obtained by lightly pressing a certain portion of the test animal or the like was recorded.

2-3) Measurement of wrinkles of skin tissue

The skin of hairless mice exposed to ultraviolet rays for 13 weeks was simulated with silicone rubber to measure the degree of wrinkle formation. A disk with a circular hole with a diameter of 1 cm was attached to the back part of the hairless mouse, and the mixture of the reagent for preparing the simulated plate was mixed and thinly spread on the back part of the hairless mouse, completely dried and then carefully peeled off the disk, Respectively. The production temperature of the simulated plate was 20 ~ 23 ℃ and the humidity was 45 ~ 50%, and the impression material (Epigem, Seoul, Korea) was used. Using the computerized image analyzer (Visioline VL650, CK electronic GmbH, Germany), the total wrinkle area, max wrinkle depth, mean depth and mean And length (mean length).

2-4) Immunohistochemical staining of skin tissue

The dorsal skin tissues of hairless mice were fixed and fixed in 10% formalin, followed by hematoxylin and eosin (H & E) staining. (ECLIPSE E600, Nikon, Japan) and photographed using a digital camera (DXM 1200F, Nickon, Japan).

2-5) RT-PCR analysis

The tissue was pulverized by adding 1 ml of the tripozol solution per 0.1 g of skin tissue, and centrifuged at 12,000 x g for 10 minutes at 4 ° C. The supernatant was transferred to a new tube, then 200 μl of chloroform was added and vortexed. This process was repeated twice, then the supernatant was transferred to a new tube and isopropanol and supernatant were added at a ratio of 1: 1. After 10 h of vigorous shaking, the mixture was allowed to stand at room temperature for 15 min. After centrifugation at 12,000 xg for 10 min at 4 ° C, the supernatant was removed, and 1 ml of 70% ethanol was added to the remaining precipitate. Lt; / RTI > for 5 minutes. After removing the ethanol, the tube containing the RNA precipitate was dried at room temperature for 15 minutes, and the RNA pellet was dissolved using nucleic acid-free water. The concentrations of RNA samples extracted at 260 nm and 280 nm wavelengths were measured using a UV / VIS spectrophotometer (Beckman coulter, DU730) and agarose gel electrophoresis was performed to confirm the integrity of the RNA samples Respectively.

(GIBCO BRL, Gaithersburg, Md., USA), and cDNA was synthesized by reverse transcription using the dT primer and superscript reverse transcriptase (GIBCO BRL, Gaithersburg, MD, USA). PCR was carried out using the cDNA obtained from the reverse transcription as a template and the 5 'and 3' flanking sequences of the gene cDNA to be amplified as primers. The primer sequences used in this procedure were as shown in [Table 3] Same as. 1 μl of the amplified PCR product was electrophoresed on 1% agarose gel to confirm the DNA band.

The primer sequence used for RT-PCR gene primer The base sequence (5 '- > 3') Annealing  Temperature
(° C) PCR
product
(bp) SEQ ID NO: Collagen type Ⅰ alpha 1 (Col1α1) F ggcaacagtcgcttcaccta 55 164 7 R agtccgaattcctggtctgg 8 Collagen type Ⅰ alpha 2 (Col1α2) F cggttctgttggtcctgttg 55 103 9 R acccctgtgccctttatcac 10 Collagen type Ⅲ alpha 1 (Col3α1) F taaccaaggctgcaagatgg 55 104 11 R accagtgcttacgtgggaca 12 Matrix metallopeptidase 1a (MMP-1a) F ccctgtgtttcacaacggag 55 133 13 R cctcagcttttcagccatca 14 Matrix metallopeptidase 1b (MMP-1b) F tttgctcatgcttttctgcc 55 146 15 R gaatgggagagtccaaggga 16 Matrix metallopeptidase 3 (MMP-3) F tgctggtatggagcttctgc 55 142 17 R catctccaacccgaggaact 18 Matrix metallopeptidase 9 (MMP-9) F gtggaccatgaggtgaacca 55 102 19 R actgcacggttgaagcaaag 20 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) F ggagattgttgccatcaacg 55 122 21 R tgacaagcttcccattctcg 22

2-6) Measurement of body weight and dietary intake of reckless mice

As shown in FIG. 3, ultraviolet irradiation, alpha-terpineol and vitamin C intake did not significantly affect body weight and dietary intake of reared mice (see FIG. 3).

2-7) Measurement of moisture content, elasticity and erythema index of ultraviolet irradiated mouse skin

As shown in FIG. 4, in the + UVB control group irradiated with ultraviolet rays for 13 weeks, the moisture content and elasticity of the skin tissue were decreased significantly compared to the UV group (-UVB), while the moisture evaporation and erythema index (See FIG. 4). In the group of α-terpineol (αTN), the water content and elasticity were significantly increased by 37% and 36%, respectively, compared with the + UVB control group, and the moisture evaporation and erythema index Were significantly reduced by 47% and 20%, respectively. The effects of α-terpineol on skin tissue moisture, elasticity and erythema index were similar to those of vitamin C (see FIG. 5).

2-8) Changes in skin wrinkle formation in ultraviolet irradiated mice

In order to evaluate the effect of α-terpineol on the degree of skin wrinkle formation, the anti-wrinkle effect of α-terpineol (αTN) The control group (+ UVB) was compared and photographed. As shown in FIG. 6, the + UVB control group was visually observed to form fine wrinkles with a lot of coarse and deep wrinkles compared to the normal UV group (-UVB) Group, the thickness and depth of the wrinkles were remarkably reduced compared with the UVB control group, and it was confirmed that the wrinkles were improved in a similar manner to the skin conditions observed in the UV-unexposed UVB group (see FIG. 5).

As a result of the measurement of the degree of wrinkle formation by using a silicone rubber foil made of the squamous epithelium of the hairless mice subjected to UV irradiation for 13 weeks, the + UVB control group was found to have thicker and deeper wrinkles than the normal UV- , And the α-terpineol-supplemented group showed remarkable improvement in the thickness and depth of the wrinkles, such that deep wrinkles almost disappeared compared with the + UVB control group even though the same UV intensity was irradiated (See FIG. 6). The results of numerical analysis of the degree of wrinkling in the simulated plate using a computerized image analyzer showed that the total wrinkle area was 40%, the maximum wrinkle depth was 65%, the average wrinkle depth was 19%, and the average wrinkle area was 40% The length was significantly reduced by 45%, and the wrinkle-reducing effect of? -Terpineol was similar to the wrinkle-reducing effect observed in vitamin C (see FIG. 6). Therefore, it can be seen that the ingestion of? -Terpineol has an effect of remarkably suppressing the generation of wrinkles by ultraviolet irradiation.

2-9) Measurement of change in skin thickness of ultraviolet irradiated mice

As the photoaging by ultraviolet rays proceeds, the formation of the stratum corneum is increased to increase the thickness of the skin skin layer to protect the dermal layer of the skin, and the thickening of the skin by ultraviolet irradiation means that the skin damage due to photoaging is large Gail J Molecular mechanism of skin aging Mech Ageing Dev 123: 801-810, 2002)

As shown in FIG. 7, when the thickness of the back skin was measured using a digital micro caliper on the last day of experimental breeding, the skin thickness of the group to which α-terpineol was added was significantly reduced by 20% as compared with the control group of + UVB (Fig. 7A). As a result of observing the skin surface layer thickness of hairless mice through H & E staining of skin tissue, the + UVB control group showed thickening of the skin skin layer compared with the normal UV group (-UVB) It was confirmed that the thickness of the thickened epidermal layer was significantly reduced as compared with the control group (see Fig. 7B).

2-10) Gene expression of ultraviolet irradiated mouse skin

Collagen types 1 and 3 are proteins that constitute cellular epilepsy components of the dermis, and type 1 collagen is present in the largest amount among extracellular matrix proteins present in skin connective tissue. Meanwhile, 23 types of MMPs catalyzing collagen degradation exist in mammals. MMP types 1, 3 and 9 are known to increase by ultraviolet rays. These three types of MMPs are enzymes that degrade collagen type 1 and 3 . While MMP-1 cleaves the middle of the collagen fibers, MMP-3 and MMP-9 are known to break down the cut collagen fibers.

As a result of RT-PCR analysis of gene expression in skin tissues, it was found that the + UVB control group had higher collagen type 1α1 and α2, and collagen type 3α1 (compared to -UVB) Expression of MMP-1a and -1b, MMP-3, and MMP-9 gene were significantly increased. The expression of collagen type 1α1 and α2 and collagen type 3α1 was significantly increased in the α-terpineol-fed group compared with the + UVB-treated group, and the expression of MMP-1a and -1b, MMP-3 and MMP-9 gene (See Fig. 8). Therefore, α-terpineol is thought to increase collagen protein synthesis in subcutaneous tissues and inhibit collagen fiber degradation, thereby alleviating wrinkle formation caused by ultraviolet irradiation.

Skin wrinkle improvement effect and skin irritation sensory test

3-1) Formulation Examples and Comparative Examples

The composition of the nutritional cream containing? -terpineol was prepared as shown in Table 4 below. Here, the unit of the component content is% by weight.

Composition of nutritional cream containing α-terpineol number ingredient Example Comparative Example One Cetearyl alcohol 1.5 1.5 2 Glyceryl stearate 1.0 1.0 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyl octanoate 6.0 6.0 6 Squalane 8.0 8.0 7 Apricode kernel oil 4.0 4.0 8 Dimethicone 1.0 1.0 9 Butylene glycol 5.0 5.0 10 glycerin 4.0 4.0 11 Magnesium aluminum silicate 0.2 0.2 12 Xanthan gum 0.05 0.05 13 antiseptic a very small amount a very small amount 14 Purified water Balance Balance 15 alpha-terpineol 1.0 -

On the other hand, among the components identified by the respective component numbers in Table 4, the components 1 to 8 are first melted by heating at a temperature of 70 캜, then the components 9 to 13 are dissolved and dispersed in the component 14, . Thereafter, the emulsified product was cooled to a temperature of 56 캜, component 15 dissolved in the separated component 9 was added, stirred, and cooled at room temperature.

The comparative example of the above-mentioned formulation example was set up so that the composition and manufacturing method of the other components except α-terpineol as component 15 proceeded in the same manner.

3-2) Wrinkle improvement effect and skin irritation sensory test

In order to evaluate the skin wrinkle improving effect and the skin irritation of the cosmetic composition for improving skin wrinkles according to the present invention, a sensory test was performed using the nutritional cream prepared in the above-mentioned Formulation Examples and Comparative Examples.

Specifically, in order to measure the effect of improving the wrinkles of the skin when the nutritional creams of the formulation examples and the formulation comparison examples shown in Table 4 were respectively applied to the skin, 20 women over 20 years old were given the nutrition cream (Control group) was used once a day for 12 weeks on the right side of the face.

In the sensory test, the effect of improving wrinkles on the skin was relatively evaluated based on the nutritional cream of the comparative example, and the sensory evaluation on the skin irritation was caused by the itching, And so on. The evaluation was carried out according to the blotting law standards of excellent (5 points), excellent (4 points), normal (3 points), poor points (2 points) and very poor points (1 point) Respectively. In Table 5, skin irritation represents a degree of no skin irritation.

Test results of skin irritation and wrinkle improvement number Skin irritation Wrinkle improvement effect Example Comparative Example Example Comparative Example One 3 4 4 4 2 5 5 5 5 3 5 5 5 4 4 4 4 5 3 5 4 4 5 4 6 3 4 5 5 7 4 4 4 4 8 4 4 4 3 9 5 5 5 4 10 4 4 4 4 11 5 5 4 4 12 5 5 4 5 13 4 4 4 4 14 4 4 5 4 15 5 4 4 5 16 5 5 4 4 17 5 5 5 4 18 4 5 4 3 19 4 5 5 5 20 4 4 4 4 Average 4.3 4.45 4.45 4.1

As shown in Table 5, the skin irritation evaluation score of the cosmetic composition of the formulation example according to the present invention was very good at 4.3, and it was confirmed that the skin irritation degree was low and skin safety was excellent similarly to the formulation comparison example there was.

In addition, it was found that the relative skin wrinkle improving effect of the cosmetic composition of the formulation example was 4.45 points, which is much better than the comparison example.

As described above, the present invention provides a cosmetic composition containing? -Terpineol as an active ingredient. The cosmetic composition according to the present invention has no skin side effects and is excellent in improving wrinkles of skin, collagen synthesis effect and collagenase-degrading enzyme, and thus is very useful for improving wrinkles of skin. It is believed that the composition containing? -Terpineol of the present invention can be utilized as a material for functional cosmetics or health functional foods in the future.

3-3) Increase skin moisture test measurement test

In order to measure the effect of improving the wrinkles of the skin when the nutritional creams of the formulation examples and the formulation comparison examples shown in Table 4 were respectively applied to the skin, 20 women over 20 years old were given the nutrition cream (test group) , And on the right side of the face, the nutritional cream (control group) of the comparative example was continuously used once a day for 12 weeks.

The conductivity of the epidermis was measured at a constant temperature and humidity (24 ° C., relative humidity 40%) two weeks after the start of application and after four weeks, eight weeks, and twelve weeks after application, The skin moisture content was measured with a skin moisture meter which measures the amount of water present in the skin by measuring the value.

The test results are shown in Table 6 below, and the results of the following Table 6 were obtained after treatment for a certain period of time (4 weeks, 8 weeks, 12 weeks) based on the coneometer value measured immediately before the start of the test The increase in the measured value is expressed as a percentage.

Water increase rate division Moisture growth rate (%) 4 weeks 8 weeks 12 weeks Test group average 43 51 65 Control mean 13 35 42

As shown in the above Table 6, it was confirmed that the skin moisturizing cream containing α-terpineol showed a remarkable increase in skin moisture content as compared with the control group.

[Production Example 1]

Manufacture of cosmetics

1-1) Manufacture of flexible lotion

Flexible lotion containing? -terpineol as an active ingredient was prepared according to a conventional method.

Component Content (% by weight) Total amount of? -Terpineol 0.1, glycerin 3.0, butylene glycol 2.0, propylene glycol 2.0, carboxyvinyl polymer 0.1, ethanol 10.0, triethanolamine 0.1, preservative, trace pigment, trace perfume and trace amount of purified water 100.0

1-2) Manufacture of nutritional cream

A nutritional cream containing? -terpineol as an active ingredient was prepared according to a conventional method. The component content is expressed as% by weight.

a mixture of at least one of α-terpineol 0.1, wax 10.0, polysorbate 60 1.5, sorbitan sesquioleate 0.5, liquid paraffin 10.0, squalane 5.0, caprylic / capric triglyceride 5.0, glycerin 5.0, butylene glycol 3.0, propylene glycol 3.0, triethanolamine 0.2, preservative, trace pigment, trace flavor and trace amount of purified water

1-3) Preparation of mask pack composition and mask pack

A composition for a mask pack containing? -terpineol as an active ingredient was prepared according to a conventional method. The component content is expressed as% by weight.

0.1, cetearic-4 5.0, cholesterol 0.3, dicetylphosphate 0.4, concentrated glycerin 2.0, macadamia oil 10.0, carboxyvinyl polymer 0.4, polyvinylpyrrolidone 0.1, , Xanthan gum 0.1, preservative 0.2, flavor 0.15. Purified water 68.05 Total 100.0

The mask pack composition thus prepared was impregnated into a nonwoven fabric (width x length, 10 x 10 cm) to prepare a mask pack product.

[Production Example 2]

Production of pharmaceutical preparations

2-1) Manufacture of powder

2 g of? -Terpineol of the present invention

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

2-2) Preparation of tablets

100 mg of? -Terpineol of the present invention

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

2-3) Preparation of capsules

100 mg of? -Terpineol of the present invention

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

2-4) Preparation of rings

1 g of? -Terpineol of the present invention

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

2-5) Preparation of granules

150 mg of? -Terpineol of the present invention

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and the mixture was dried at 60 캜 to form granules, which were then filled in a capsule.

[Production Example 3]

Manufacturing of food products

The functional food composition containing? -terpineol was prepared by a usual liquid preparation method with the same composition as each of the following formulation examples. The final volume is 100 ml for each liquid. This preparation example can be prepared not only as a liquid preparation but also tablets, powders, granules and the like by a usual production method.

Ingredients (wt%) α-terpineol 100 mg, honey 1500 mg, vitamin C 50 mg, vitamin B 6 10 mg, nicotinamide 10 mg, royal jelly 80 mg, preservative, incense,

<110> Industry-Academic Cooperation Foundation, Yonsei University <120> Composition for making alpha-terpineol for moisturizing and          improving skin wrinkle <130> PB15-12970 <160> 22 <170> Kopatentin 2.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Procollagen forward primer <400> 1 tcttcaagcc atcctgtgtg 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Procollagen reverse primer <400> 2 gcgagtctgt gtttttgcag 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Metalloproteinase 1 forward primer <400> 3 atgacatgag tccggagcaa 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Metalloproteinase 1 reverse primer <400> 4 tcatctcctg ggtccctttc 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Glyceraldehyde 3-phosphate dehydrogenase forward primer <400> 5 gtgatggcat ggactgtggt 20 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Glyceraldehyde 3-phosphate dehydrogenase reverse primer <400> 6 ggagccaaaa gggtcatcat 20 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Collagen type 1 alpha 1 forward primer <400> 7 ggcaacagtc gcttcaccta 20 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Collagen type 1 alpha 1 reverse primer <400> 8 agtccgaatt cctggtctgg 20 <210> 9 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Collagen type 1 alpha 2 forward primer <400> 9 cggttctgtt ggtcctgttg 20 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Collagen type 1 alpha 2 reverse primer <400> 10 acccctgtgc cctttatcac 20 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Collagen type 3 alpha 1 forward primer <400> 11 taaccaaggc tgcaagatgg 20 <210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Collagen type 3 alpha 1 reverse primer <400> 12 accagtgctt acgtgggaca 20 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 1a forward primer <400> 13 ccctgtgttt cacaacggag 20 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 1a reverse primer <400> 14 cctcagcttt tcagccatca 20 <210> 15 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 1b forward primer <400> 15 tttgctcatg cttttctgcc 20 <210> 16 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 1b reverse primer <400> 16 gaatgggaga gtccaaggga 20 <210> 17 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 3 forward primer <400> 17 tgctggtatg gagcttctgc 20 <210> 18 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 3 reverse primer <400> 18 catctccaac ccgaggaact 20 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 9 forward primer <400> 19 gtggaccatg aggtgaacca 20 <210> 20 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Matrix metallopeptidase 9 reverse primer <400> 20 actgcacggt tgaagcaaag 20 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Glyceraldehyde 3-phosphate dehydrogenase forward primer <400> 21 ggagattgtt gccatcaacg 20 <210> 22 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Glyceraldehyde 3-phosphate dehydrogenase reverse primer <400> 22 tgacaagctt cccattctcg 20

Claims (18)

terpineol as an active ingredient and having at least one effect selected from the group consisting of an increase in procollagen secretion amount, promotion of collagen biosynthesis, reduction of expression of MMP-1 gene, and inhibition of skin skin layer thickening To improve skin wrinkles and skin elasticity.
The method according to claim 1,
Wherein the? -Terpineol is contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic composition.
delete The method according to claim 1,
The cosmetic may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, a moisturizing cream, a hand cream, Wherein the composition has one of the formulations selected from the group consisting of a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder and an eye shadow. / RTI &gt;
The method according to claim 1,
Wherein the composition further comprises a cosmetically acceptable carrier. 2. The cosmetic composition according to claim 1, wherein the composition further comprises a cosmetically acceptable carrier.
The method according to claim 1,
Wherein the composition further comprises at least one skin wrinkle-improving component selected from the group consisting of vitamin C, retinoic acid, TGF, animal placenta-derived protein, betulinic acid and chlorella extract. And improving skin elasticity.
delete terpineol as an active ingredient and having at least one effect selected from the group consisting of an increase in procollagen secretion amount, promotion of collagen biosynthesis, reduction of expression of MMP-1 gene, and inhibition of skin skin layer thickening Wherein the skin is moisturized, the skin is wrinkled, and the skin elasticity is improved.
9. The method of claim 8,
The food composition according to any one of claims 1 to 3, wherein the food is prepared from any one selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings.
terpineol as an active ingredient and having at least one effect selected from the group consisting of an increase in procollagen secretion amount, promotion of collagen biosynthesis, reduction of expression of MMP-1 gene, and inhibition of skin skin layer thickening Which is used for skin moisturizing, skin wrinkle improvement and skin elasticity enhancement. delete delete delete delete delete delete delete delete

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