KR20190130543A - Composition for improving wrinkle comprising Symplocarpus foetidus extract or fractions thereof - Google Patents

Abstract

The present invention relates to a composition for wrinkle relief or prevention, containing a skunk cabbage extract or a fraction thereof as an active ingredient. Specifically, the present invention relates to a cosmetic composition, a food composition, a pharmaceutical composition, and a quasi-drug composition each containing a skunk cabbage extract or a fraction thereof as an active ingredient, and to a method for wrinkle relief, the method comprising a step of administering the composition to a subject other than a human being.

Description

Composition for improving wrinkle comprising Symphonic acid extract or fractions thereof as active ingredient {Composition for improving wrinkle comprising Symplocarpus foetidus extract or fractions}

The present invention relates to a composition for preventing or improving wrinkles, which comprises a sitting fan extract or a fraction thereof as an active ingredient. Specifically, the present invention relates to a cosmetic composition, a food composition, a pharmaceutical composition, and a quasi-drug composition comprising a sitting fan extract or a fraction thereof as an active ingredient, and a method for improving wrinkles comprising administering the composition to an individual except a human.

Wrinkles can be divided into facial wrinkles caused by facial muscles used for facial expression and fine wrinkles generated generally or locally. Muscles that cause facial wrinkles contract and relax by the action of acetylcholine, a neurotransmitter that is secreted from the nerve cell ends of neuromuscular junctions. Botoxin (Botulinum neurotoxin) is the most powerful substance used to improve the appearance of wrinkles, and induces membrane fusion of synaptic vesicles and presynaptic cells of neurons to release neurotransmitters. The cleavage of SNAP-25 in the soluble Nethylmaleimide-sensitive factor attachment protein receptor (SNAP Receptor), a protein complex that regulates protein, blocks SNARE complex formation and consequently prevents the release of acetylcholine to paralyze muscle cells. However, as the mechanism of action of botox is an irreversible cleavage of the SNARE complex, and safety issues continue to be suggested by the fact that it is a toxic substance, synthetic peptides such as agireline, which are involved in the mechanism of action of botox, have been developed (Blanes). Mira etc., International Journal of Cosmetic Science. 24, 303-310, 2002). However, azireline has a price-effectiveness question and a synthetic weakness.

Unlike facial wrinkles, fine wrinkles are known to be caused by a decrease in collagen, a major component of the extracellular matrix. Collagen is the major matrix protein produced in skin fibroblasts and accounts for about 30% of the total weight of biological proteins. The main functions of collagen are known as mechanical firmness of skin, resistance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation (in organic growth or wound healing). In addition, collagen is a substrate protein that plays an important role in the retention of moisture in the dermal layer, and the substrate protein absorbs moisture, thereby increasing the retention of moisture inside the structure of the structure that allows the skin to maintain the proper moisture content, It is known to be involved in maintaining the elasticity of the skin. This collagen is reduced by age and photoaging by ultraviolet irradiation, which is known to be closely associated with wrinkle formation of the skin. In order to solve this problem, interest in collagen synthesis promoting material has been increased, and conventionally known collagen synthesis promoting materials include vitamin C, retinoic acid, protein from animal placenta, betulinic acid and chlorella Extracts. However, these materials have limited usage due to safety problems such as irritation and redness when applied to the skin, or the effect is insignificant, so that the effect of improving skin wrinkles could not be expected. Therefore, there is an urgent need for the development of new anti-wrinkle and improved external skin composition that is safer and more effective for the body than the existing external skin composition.

Under these circumstances, the present inventors have made diligent researches on substances showing excellent skin improvement effects from natural resources existing in nature, and found that sitting fan extract exhibited an effect of reducing muscle contraction and increasing total amount of collagen biosynthesis. The present invention has been completed.

(Patent Document 1) Republic of Korea Patent Publication No. 2015-0143375

(Patent Document 2) Republic of Korea Patent Publication No. 2015-0009266

It is a main object of the present invention to provide a cosmetic composition for preventing or improving wrinkles comprising a sitting fan extract or a fraction thereof as an active ingredient.

It is another object of the present invention to provide a functional food composition for preventing or improving wrinkles comprising a sesame extract or a fraction thereof as an active ingredient.

Still another object of the present invention is to provide a pharmaceutical composition for preventing or improving wrinkles, which comprises a sitting fan extract or a fraction thereof as an active ingredient.

Still another object of the present invention is to provide a quasi-drug composition for preventing or improving wrinkles, comprising a sedentary fan extract or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a method for improving wrinkles comprising administering the composition to a subject other than a human.

The present inventors have conducted various studies using substances exhibiting excellent skin wrinkle improvement effects from natural resources existing in nature. As a result, it was confirmed that the sitting fan extract exhibited the effect of reducing muscle contraction and increasing the total amount of collagen biosynthesis. It was found that the sitting fan extract can be used as a cosmetic composition for improving wrinkles. The cosmetic composition comprising the sedentary fan extract having the wrinkle improvement effect is not known at all, it can be said that the significance is very significant in that it was first developed by the present inventors.

In one aspect, in order to solve the above problems, it provides a cosmetic composition for preventing or improving wrinkles comprising a sitting fan extract or a fraction thereof as an active ingredient.

The term "symplocarpus foetidus" used in the present invention is a perennial herb belonging to the genus Cheknamseong and perching fan, which is distributed in North America, eastern Siberia, and East Asia, and in Korea, in the shaded areas of the country except Jeju Island. Grow up There is no stem and flowers bloom before leaves in February-April. A small flower grows in the fluorescete, an oval flower cover shaped like a pouch with red stains. The fruit is berry and ripens in summer in red.

It is known that the sitting fan is mainly sedative, antipyretic effect, but the inventors first identified the wrinkle improvement effect of the sitting fan. For the purposes of the present invention, the seated fan is not limited to the type, and any seated fan can be used without limitation in its source, such as using a cultivated one or a commercially purchased one.

In the present invention, "extract" is obtained by removing the solvent from the liquid component obtained by immersing the desired material in various solvents, and then extracting for a predetermined time at room temperature, low temperature or warm state, It means a result such as a solid content. In addition, in addition to the result, it can be comprehensively interpreted to include all of the dilution of the resultant, their concentrates, their modifiers, purified products and the like.

In the present invention, the extract means sitting fan extract. The sitting fan extract can be extracted from various organs of natural, hybrid, and mutant plants, and can be extracted from plant tissues as well as roots, ground, stems, leaves, flowers, trunks of fruits, peels of berries. The sitting fan extract can be obtained by extracting with water or various organic solvents. At this time, the organic solvent used is not particularly limited as long as the extract can be obtained, but may be specifically water, polar solvent or non-polar solvent, more specifically water, lower alcohol having 1 to 6 carbon atoms ( Methanol, ethanol, propanol or butanol, and the like, and a mixed solvent thereof, and most specifically, methanol or a mixed solvent thereof.

In addition, the method for obtaining the extract is not particularly limited as long as it can obtain a sitting fan extract, specifically, the roots, stems, leaves, berries, flowers, dried products, processed products, etc. of the sitting fan. Immersion in the solvent, cold extraction method extracted at room temperature, heat extraction method, ultrasonic extraction method by applying an ultrasonic wave, reflux extraction method using a reflux cooler and the like can be used.

As used herein, the term "fraction" refers to the result obtained by performing fractionation to separate a specific component or a specific group of components from a mixture comprising several different components.

The fractionation method of obtaining the fraction in the present invention is not particularly limited as long as it can exhibit a skin wrinkle improvement effect, it may be performed according to a method commonly used in the art. For example, solvent fractionation is performed by treating various solvents, ultrafiltration fractionation is carried out through an ultrafiltration membrane having a constant molecular weight cut-off value, and separation according to various chromatography (size, charge, hydrophobicity or affinity) is performed. Chromatography), and combinations thereof.

The kind of the fractionation solvent used to obtain the fraction in the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvents include polar solvents such as water, distilled water and alcohols; And nonpolar solvents such as hexane, ethyl acetate, chloroform, dichloromethane, and the like. These may be used alone or in combination of two or more thereof. In the case of using alcohol in the fractionation solvent, alcohols of C1 to C4 may be preferably used.

The cosmetic composition of the present invention may include 0.0001 to 10% by weight of the extract or fractions thereof, and specifically 0.0005 to 5% by weight.

If the content is less than 0.0001% by weight, it is not preferable because the original desired activity can not be sufficiently achieved, and if it exceeds 10% by weight it is not expected to increase the apparent effect as increasing content may be uneconomical.

In the present invention, the composition may be characterized in that to improve the skin wrinkles.

The term "wrinkle" as used above can be divided into facial wrinkles caused by facial muscles used to make a facial expression and fine wrinkles caused by skin deterioration generally or locally. Specifically, wrinkles may be caused by genes, a decrease in collagen present in skin dermis, external environment, and the like. For the purposes of the present invention, wrinkles may be used to include both facial wrinkles and fine wrinkles, but are not limited thereto.

In the present invention, the composition may be characterized in that to reduce the muscle contraction to relieve facial wrinkles.

According to one embodiment of the present invention, as a result of treating the sitting fan extract by concentration, it was confirmed that muscle contraction is reduced in a concentration-dependent manner.

Through this, it was found that the sitting fan extract can improve the facial wrinkles.

In addition, in the present invention, the composition may be to promote collagen synthesis to increase the total amount of collagen.

According to one embodiment of the present invention, it was confirmed that the sitting fan extract has the effect of promoting collagen synthesis to increase the total amount of collagen

Through this, it was found that the sitting fan extract can improve the fine wrinkles.

As used herein, the term "improvement" refers to alleviating the symptoms of wrinkles of the skin using the composition of the present invention, "prevention" includes both to prevent the formation of wrinkles, or to delay the formation of wrinkles do.

The cosmetic composition according to the present invention is a solution, external ointment, cream, foam, nourishing lotion, softening lotion, pack, softening water, latex, makeup base, essence, soap, liquid cleansing agent, bath, sunscreen cream, sun oil, suspension, Emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays may be prepared in a formulation selected from the group consisting of, but not limited to It is not.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as conventional components, for example, oil, water, surfactants, moisturizers, lower alcohols, Thickeners, chelating agents, pigments, preservatives, flavoring agents, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the dosage form.

When the formulation of the present invention is an ointment, paste, cream or gel, the carrier component may be animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these may be used.

If the formulation of the invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or mixtures thereof may be used, in particular in the case of a spray additionally chloro Propellants, such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, solvents, solubilizers or emulsions are used as carrier components, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, in particular cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, fatty acid esters of glycerol aliphatic ester, polyethylene glycol or sorbitan may be used. have.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, micro Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like may be used as carrier components. Can be.

The cosmetic composition of the present invention is an adjuvant commonly used in the sitting fan extract or fractions thereof such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants And dyes and the like.

As another aspect for achieving the above object, the present invention provides a functional food composition for preventing or improving wrinkles as an active ingredient of a sitting fan extract or a fraction thereof.

As used herein, the terms "sitting fan", "extract", "fragment", "wrinkle", "prevention" and "improvement" are as described above.

In the present invention, the functional food composition may have an effect such as skin wrinkle improvement, but is not limited thereto.

The functional food composition comprising the sitting fan extract or a fraction thereof contained in the functional food composition of the present invention as an active ingredient may be included in the form of an extract thereof, a fraction thereof or a processed product thereof. In addition, the composition may contain a food supplement acceptable food additives in addition to the active ingredient.

In the present invention, the "food supplement" means a component that can be added to food supplements, and can be appropriately selected and used by those skilled in the art as being added to prepare the health functional food of each formulation. Examples of food additives include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners. , pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like, but is not limited to the kind of food additives of the present invention by the above examples.

Functional food composition of the present invention may include a health functional food. In the present invention, the "health functional food" refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, the term "functionality" means obtaining useful effects on health purposes such as nutrient control or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and the preparation can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. Food composition of the present invention can be prepared in various forms of formulation, unlike the general medicine has the advantage that there is no side effect that can occur when taking a long-term use of the drug as a food raw material, excellent portability, Health functional foods can be taken as supplements to enhance skin wrinkles.

There is no restriction on the form that the health functional food of the present invention can take, and may include all foods in a general sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention may be prepared by mixing known additives with other appropriate auxiliary ingredients that may be included in the food according to the choice of those skilled in the art. Examples of foods that can be added include meat, sausages, breads, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products, including ice cream, various soups, beverages, teas, drinks, alcoholic beverages and Vitamin complexes, and the like, can be prepared by adding the compound represented by the formula (1) according to the invention as a main ingredient juice, tea, jelly and juice. It also includes foods used as feed for animals.

As another aspect, the present invention provides a pharmaceutical composition for preventing or improving wrinkles, comprising a sesame fan extract or a fraction thereof as an active ingredient.

As used herein, the terms "sitting fan", "extract", "fragment", "wrinkle", "prevention" and "improvement" are as described above.

The pharmaceutical composition of the present invention may also be used as a single agent, and may be prepared and used as a complex preparation, further comprising a drug known to have an effect of improving wrinkles or skin aging, and may be formulated using a pharmaceutically acceptable carrier or excipient. It may be prepared in unit dose form or incorporated into a multi-dose container.

The pharmaceutical composition may be administered in a pharmaceutically effective amount.

The "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is determined by the type and severity of the individual, age, sex, activity of the drug, drug Sensitivity, time of administration, route of administration and rate of release, duration of treatment, factors including concurrent use of drugs, and other factors well known in the medical arts.

As another aspect for achieving the above object, the present invention provides a quasi-drug composition for preventing or improving wrinkles comprising as an active ingredient comprising a sesame fan extract or a fraction thereof as an active ingredient.

The quasi-drug composition in the present invention may have a skin wrinkle improvement effect, but is not limited thereto.

As used herein, the terms "sitting fan", "extract", "fragment", "wrinkle", "prevention" and "improvement" are as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as necessary in addition to the above components. The pharmaceutically acceptable carriers, excipients or diluents are not limited so long as they do not impair the effects of the present invention. For example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It may include.

The "pharmaceutically acceptable carrier" may refer to a carrier, excipient or diluent that does not impair the biological activity and properties of the compound to be injected, without stimulating the organism, specifically, non-naturally occuring carrier). The kind of the carrier usable in the present invention is not particularly limited, and any carrier can be used as long as it is a conventionally used and pharmaceutically acceptable carrier in the art. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and the like. These may be used alone or in combination of two or more thereof.

The composition comprising a pharmaceutically acceptable carrier may be in various oral or parenteral formulations. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose, lactose, It can be prepared by mixing gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc can also be used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, utopsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The quasi-drug composition of the present invention may be exemplified by disinfecting detergents, shower foams, ointments, wet tissues, coatings, etc., but is not limited thereto. Formulation methods, dosages, methods of use, components, etc. of the quasi-drugs are well known in the art. Can be appropriately selected from the description of.

In addition, the quasi-drug composition comprising the sedentary fan extract of the present invention or an active ingredient comprising a fraction thereof may be used in a method for improving skin wrinkles, including applying to the skin of an individual. The subject includes, without limitation, mammals including rats, livestock, humans and the like.

In another aspect, there is provided a method for improving wrinkles comprising administering to a subject a composition comprising a sessock extract or a fraction thereof.

As used herein, the terms "sitting fan", "extract", "fraction", and "wrinkle" are as described above.

As used herein, the term "administration" means introducing a composition of the present invention to a patient in any suitable manner, and the route of administration of the composition may be administered via any general route as long as it can reach the desired tissue. . Intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, but is not limited thereto. Due to the properties of the composition of the present invention is effective in improving skin wrinkles, the route of administration of the composition may be administered by applying to the skin.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into two or three times. In addition, the composition of the present invention can be used alone or in combination with other drug treatments to improve skin wrinkles. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect in a minimum amount without side effects, and can be easily determined by those skilled in the art.

As used herein, the term "individual" means all animals, such as rats, mice, and livestock, including humans, in which wrinkles or skin wrinkles may occur or may occur. Specifically, it may be a mammal including a human.

Sitting fan extract according to the present invention is excellent in the effect of reducing muscle contraction, and the total amount of collagen biosynthesis, it can be used in cosmetics, medicines and quasi-drugs for skin wrinkle improvement.

1 is a graph showing the effect of inhibiting muscle contraction according to the concentration of sesame lichen extract.
Figure 2 is a graph measuring the total amount of collagen biosynthesis according to the concentration of sitting fan extract.
Figure 3 is a diagram showing the visual evaluation of the forehead wrinkles using DSLR after using the essence containing 2% of the sitting fan extract.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Example  1: Preparation of sitting fan extract

In order to prepare an extract of the sitting fan collected from Daedunsan, 10 g of the sitting fan was crushed and placed in a flask and extracted by cooling with 100 g of an extraction solvent (distilled water: methanol = 3: 7) for 3 days. The cooled extract was filtered with a filter having a pore size of 0.2 μm to prepare a sitting fan extract.

Example  2: Determination of Wrinkle Improvement Activity of Lichen Radish Extract

2-1: Experiment of neuromuscular junction formation and measurement of muscle contraction inhibitory effect

In order to verify the effect of suppressing muscle contraction of the sitting fan, mouse-derived muscle cell line C2C12 and NG108-15, a hybrid neuronal cell line of mouse and rat, were co-cultured.

C2C12, a muscle cell line, was dispensed in 6-well microplates at 5 × 10 5 cells per well, incubated for 24 hours at 10% bovine serum DMEM medium and 37 ° C., and then the medium was removed and once with phosphate buffer solution. After washing, the cells were differentiated by exchanging with 2% horse serum DMEM medium. Two days later, 2% horse serum DMEM medium was exchanged and cultured for three more days to divide the NG108-15 neuronal cell line into 5 × 10 4 cells on differentiated muscle cells. Incubated for hours. Thereafter, the medium of the neuromuscular junction cell model (C2C12 and NG108-15 co-culture) was removed and washed once with phosphate buffer solution, and then serum-free DMEM medium and control group were added with the sessile fan extract prepared in Example 1. Serum-free DMEM media without sedentary fan extract was treated for 2 hours.

The optical shrinkage (Zeiss, Oberkochen, Germany) was used to measure the number of muscle contractions before and after 2 hours of treatment. The number of muscle contractions was calculated by counting the number of contractions for 30 seconds at three locations.

As a result, as shown in Figure 1 and Table 1, it was confirmed that the sitting fan extract reduced muscle contraction in a concentration-dependent manner after 2 hours treatment, in particular, the effect of reducing the number of muscle contractions of about 47% at 20ppm sitting fan extract It confirmed that it was shown.

Sitting fan extract (ppm) Muscle contraction reduction rate (%) 0 One One 19 5 22 10 25 20 47

This means that facial wrinkles formed by continuous muscle use can be alleviated.

2-2: Increased total amount of collagen biosynthesis

Sitting fan extract was added to the culture solution of human-derived fibroblasts to test the effect of increasing the total amount of collagen biosynthesis at the cellular level.

Specifically, the measurement of the total amount of collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT). Evaluation of cytotoxicity by varying the concentration (μg / ml) of the sedentary fan extract prepared in Example 1 in human-derived fibroblasts before the experiment (method of culturing fibroblasts and performing the MTT test [Reference: Mossman T. (1983) .Rapid Colorimetric Assay for Cellular Growth & Survival: application to proliferation & cytotoxicity assays.Journal of Immunological Methods 65, 55-63]). The degree was evaluated.

After each sample was added to the culture medium of human fibroblasts and cultured for 3 days, the culture medium was taken, and the degree of increase in total amount of collagen at each concentration was measured at 450 nm using a spectrophotometer with PICP EIA kit. In order to compare the effects, the increase in the total amount of collagen was measured in the same manner with respect to the sample added with 10 ng / ml of the culture medium (control) and TGF (positive control) of the fibroblasts to which nothing was added. The total amount of collagen was measured as UV absorbance, and the increase rate of the total amount of collagen was calculated as a ratio of the total amount of collagen relative to the control group, and the results are shown in FIG. 2 and Table 2 below.

As a result, as shown in Figure 2 and Table 2, it was confirmed that the sitting fan extract has the effect of promoting collagen synthesis to increase the total amount of collagen.

sample % Collagen synthesis increase compared to control Sitting fan extract (ppm) One 0.6 5 7.8 10 10.4 20 10.3

This means that a reduction in collagen can alleviate the fine lines that are known to be the cause.

Example  3: containing a sitting fan Cosmetic  Wrinkle improvement effect measurement

Forehead wrinkles are wrinkles formed by a combination of facial wrinkles formed by continuous muscle use and fine wrinkles due to aging, and the effect of improving the forehead wrinkles of the sitting fan extract according to the present invention was investigated.

Specifically, the essence containing 2% of the sesame fan extract prepared in Example 1 was used for 12 healthy women aged 30 to 45 years, twice a day on the forehead of each subject for 1 month. The control group used an essence that does not contain sedentary fan extract. Wrinkle improvement was measured through a photo evaluation and questionnaire evaluation using a DSLR camera (Nikon DSLR 3100), the results are shown in Figure 3 and Table 3.

As can be seen in Figure 3, in the experimental group using the sitting fan extract 2% according to the present invention was confirmed that the wrinkles significantly improved the results of the photograph determination. In addition, 4 weeks after the questionnaire, the questionnaire asked about improvement of skin wrinkles and feeling of skin tightening. The average score was over 4 points in the experimental group, and the average score over 3 points in the control group. Essence containing it was confirmed to feel fine wrinkles and facial wrinkles improved (Table 3).

Questionnaire Experimental group Control 2 weeks 4 Weeks 2 weeks 4 Weeks Forehead skin wrinkles seem to improve. 4.0 4.3 2.8 3.2 Forehead skin feels tight. 4.8 4.2 3.5 3.5

1 point: Not at all. 2 points: No

3 points: a little bit 4 points: a little bit

5 points: Yes 6 points: Yes

As a result, as shown in Table 3, when using the essence containing the sitting fan extract of Example 1, the forehead wrinkles (face wrinkles and fine wrinkles) is improved compared to the control group does not contain the sitting fan extract Confirmed.

From the above description, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, the embodiments described above are to be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present invention.

Claims (12)

A cosmetic composition for preventing or improving wrinkles, comprising a fraction of sesame lichen extract as an active ingredient.
The method of claim 1,
The wrinkles are facial wrinkles or fine wrinkles, cosmetic composition.
The method of claim 1,
The extract is prepared by extracting with a solvent selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, and mixed solvents thereof.
The method of claim 1,
Fraction of the sitting fan extract will comprise 0.0001 to 10% by weight based on the total weight of the composition, cosmetic composition.
The method of claim 1,
The composition is characterized in that to reduce muscle contractions to relieve facial wrinkles, cosmetic composition.
The method of claim 1,
The composition is characterized in that to promote collagen synthesis to increase the total amount of collagen, cosmetic composition.
The method of claim 1,
The composition is selected from softening water (nutrition fluid), astringent lotion, nutrient cream, nutrition cream, massage cream, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, suspension, emulsion, spray or cosmetic liquid It is a formulation, cosmetic composition.
The method of claim 1,
The composition further comprises at least one carrier selected from the group consisting of oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives and flavorings.
Functional food composition for the prevention or improvement of wrinkles using a fraction of the sitting fan extract as an active ingredient.
Wrinkle prevention or improvement pharmaceutical composition comprising a fraction of the sesame fan extract as an active ingredient.
A quasi-drug composition for preventing or improving wrinkles, comprising a fraction of sesame lichen extract as an active ingredient.
A method for improving wrinkles, comprising administering to a subject, except a human, a composition comprising a fraction of sesame lichen extract.

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