KR102503007B1 - A composition for promoting the differentiation of human adipose derived stem cell - Google Patents

Abstract

The present invention includes Formononetin, Emodin, Artepillin C, Carnosic acid, Palmitamide, Sodium Guaiazulene Sulfonate, Caffeic Acid, Acetyl Mandelic Acid, Laccaic Acid, Ubiquinone, Hydroxycinnamic Acid, Xanthophyll, Zucchini Extract, Aloe Extract, Zucchini A cosmetic composition, a food composition, a pharmaceutical composition, a quasi-drug composition, and a human adipose-derived stem cell composition comprising at least one material selected from the group consisting of a fraction of an extract and a fraction of an aloe extract as an active ingredient, for increasing skin elasticity and improving wrinkles It relates to a composition for promoting differentiation and a method for increasing skin elasticity, comprising administering the composition to a non-human subject.
The composition of the present invention can be used for anti-aging purposes by promoting the differentiation of human adipose-derived stem cells into adipocytes, thereby strengthening the subcutaneous fat layer structure, increasing skin elasticity, and improving wrinkles.

Description

A composition for promoting the differentiation of human adipose stem cells {A composition for promoting the differentiation of human adipose derived stem cell}

The present invention includes Formononetin, Emodin, Artepillin C, Carnosic acid, Palmitamide, Sodium Guaiazulene Sulfonate, Caffeic Acid, Acetyl Mandelic Acid, Laccaic Acid, Ubiquinone, Hydroxycinnamic Acid, Xanthophyll, Zucchini Extract, Aloe Extract, Zucchini A cosmetic composition, a food composition, a pharmaceutical composition, a quasi-drug composition, and a human adipose-derived stem cell composition comprising at least one material selected from the group consisting of a fraction of an extract and a fraction of an aloe extract as an active ingredient, for increasing skin elasticity and improving wrinkles It relates to a composition for promoting differentiation and a method for increasing skin elasticity, comprising administering the composition to a non-human subject.

Adipose tissue plays a supporting role in the skin structure along with muscles, and the number of fat cells is 30-40%, but it is an important cell in terms of skin structure, accounting for 90% in volume. It is known that subcutaneous adipose tissue decreases with aging of an individual, and accordingly, skin elasticity decreases and wrinkles are formed. In addition, there are many cases in which the elasticity of the facial part decreases and wrinkles increase when fat is reduced due to rapid dieting. Therefore, increasing the volume in the skin by controlling the number of adipose tissue that plays an important role in maintaining the structure in the skin can be a way to improve skin elasticity and wrinkles. However, in the case of fat transplantation, which is currently used to improve skin elasticity, the transplanted stem cells have a very low engraftment rate in vivo, and there are problems such as requiring reoperation after a certain period of time and clumping of the transplanted fat. Therefore, it is essential to study materials capable of strengthening the structure of the subcutaneous fat layer without such surgery.

On the other hand, among the skin, the lips are not only the most mobile part among the body parts by repeating constant contraction and expansion, but are always exposed to extreme environments and are easily affected by environmental factors such as ultraviolet rays, cold, and dryness. In addition, lips have a different structure from normal skin, so their self-defense ability is weak, and as they age, their volume decreases and wrinkles increase, losing their attractive shape. Therefore, as in general skin, it is necessary to develop a composition that has an excellent effect on volume and wrinkle care in the case of lips, but the currently developed technology is a technology that uses the wrinkle improvement component studied for facial skin as it is, temporary volume There are only skills that grant increases. The latter volume increase phenomenon has a limitation in that it is caused by a stimulus response.

Recently, in studies related to fat cells or fat transplantation, adipose-derived stem cells, which are present in subcutaneous fat tissue and have the ability to differentiate into fat cells, are attracting attention. This is because adipokine secreted by adipose-derived stem cells is known to not only increase collagen synthesis in the dermal layer but also affect skin regeneration. In the case of conventional substances known to differentiate preadipocytes into adipocytes, the efficiency is low when applied to human adipose-derived stem cells as a result of using mouse preadipocytes as a target.

Accordingly, the inventors of the present invention made diligent efforts to find a substance that effectively promotes the differentiation of adipose-derived stem cells into adipocytes, and as a result, formononetin ( Formononetin), Emodin, Artepillin C, Carnosic acid, Palmitamide, Sodium Guaiazulene Sulfonate, Caffeic Acid, Acetyl Mandelic Acid, Laccaic Acid, Ubiquinone, Hydroxycinnamic Acid, Xanthophyll, Zucchini Extract, or Aloe Extract were developed, and the substances The present invention was completed by confirming that it can be used for the purpose of increasing elasticity and improving wrinkles.

Korean Publication No. 10-2012-0140450 A

One object of the present invention is formononetin, emodin, artepillin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate Guaiazulene Sulfonate, Caffeic Acid, Acetyl Mandelic Acid, Laccaic Acid, Ubiquinone, Hydroxycinnamic Acid, Xanthophyll, Sulfur Extract, To provide a cosmetic composition for enhancing skin elasticity and improving wrinkles, comprising at least one material selected from the group consisting of an aloe extract, a fraction of a pumpkin extract, and a fraction of an aloe extract as an active ingredient.

Another object of the present invention is formononetin, emodin, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, To provide a food composition for increasing skin elasticity and improving wrinkles, containing at least one material selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract as an active ingredient.

Another object of the present invention is formononetin, emodin, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, To provide a pharmaceutical composition for increasing skin elasticity and improving wrinkles, comprising at least one substance selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract as an active ingredient.

Another object of the present invention is formononetin, emodin, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, To provide a quasi-drug composition for increasing skin elasticity and improving wrinkles, comprising at least one substance selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract as an active ingredient.

Another object of the present invention is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid To provide a composition for promoting the differentiation of human adipose-derived stem cells, containing as an active ingredient at least one substance selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract. .

Another object of the present invention is to provide a method for increasing skin elasticity, comprising administering the composition to a non-human subject.

A detailed description of this is as follows. Meanwhile, each description and embodiment disclosed in this application may also be applied to each other description and embodiment. That is, all combinations of various elements disclosed in this application fall within the scope of this application. In addition, the scope of the present application is not to be construed as being limited by the specific descriptions described below.

One embodiment of the present invention is formononetin (Formononetin), emodin (Emodin), artefillin (Artepillin C), carnosic acid (Carnosic acid), palmitamide (Palmitamide), sodium guaiazulene sulfonate (Sodium Guaiazulene Sulfonate, Caffeic Acid, Acetyl Mandelic Acid, Laccaic Acid, Ubiquinone, Hydroxycinnamic Acid, Xanthophyll, Sulfur Extract, Provided is a cosmetic composition for increasing skin elasticity and improving wrinkles, comprising at least one material selected from the group consisting of an aloe extract, a fraction of a pumpkin extract, and a fraction of an aloe extract as an active ingredient. The composition includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 of the above materials. One or 16 substances may be included as active ingredients.

One embodiment of the present invention may be a cosmetic composition for increasing lip skin elasticity and improving wrinkles comprising at least one material selected from the group consisting of a silver leaf extract and a fraction of a silver leaf extract.

In addition, another embodiment of the present invention is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxyl Provided is a cosmetic composition for increasing skin elasticity and improving wrinkles, comprising a cosmetically acceptable salt of cinnamic acid or xanthophyll as an active ingredient.

In the present invention, "cosmetically acceptable salt" is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone , In the form of a salt in which hydroxycinnamic acid or xanthophyll is combined with another substance, it means a substance that can exhibit an activity similar to that of the compound cosmetically, but is not limited thereto.

The inventors of the present invention have made diligent efforts to develop substances capable of activating and restoring the aged subcutaneous fat layer, and as a result, formononetin, emodin, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeine Acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, xanthophyll, extracts of silver leaf extract, and extracts of aloe were developed to promote the differentiation of human adipose-derived stem cells into adipocytes. Through this, it was found that the composition containing the material can be used as a cosmetic composition for increasing skin elasticity and improving wrinkles. The effect of increasing skin elasticity and reducing wrinkles of the above material has not been known at all until now, and it can be said that its significance is very great in that it was developed for the first time by the present inventors.

The molecular formula of the term "formononetin" of the present invention is C ₁₆ H ₁₂ O ₄ , and the molecular weight is 284.26, which can be represented by Formula 1 below.

[Formula 1]

Formononetin of the present invention can be isolated from plants including licorice, but is not limited to the method for obtaining it, and can be chemically synthesized by a method known in the art or a commercially available material can be used.

The molecular formula of the term "Emodin" of the present invention is C ₁₅ H ₁₀ O ₅ , The molecular weight is 270.24, which can be represented by Formula 2 below.

[Formula 2]

The emodine of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The molecular formula of the term "Artepillin C" of the present invention is C ₁₉ H ₂₄ O3, and the molecular weight is 300.40, which can be represented by Formula 3 below.

[Formula 3]

Artepillin C of the present invention is not limited in its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The molecular formula of the term "Carnosic acid" of the present invention is C ₂₀ H ₂₈ O ₄ , and the molecular weight is 332.44, which can be represented by Formula 4 below.

[Formula 4]

The carnosic acid of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The term "palmitamide" of the present invention is a normal chain fatty acid amide having 16 carbon atoms, a molecular formula of C ₁₆ H ₃₃ NO, a molecular weight of 255, and may be represented by Formula 5 below.

[Formula 5]

The palmitamide of the present invention is not limited in its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The molecular formula of the term "sodium guaiazulene sulfonate" of the present invention is C ₁₅ H ₁₇ NaO ₃ S, and the molecular weight is 300.35, which can be represented by Formula 6 below.

[Formula 6]

The sodium guaiazulene sulfonate of the present invention is not limited in its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The molecular formula of the term "caffeic acid" of the present invention is C ₉ H ₈ O ₄ , and the molecular weight is 180.157, which can be represented by Formula 7 below.

[Formula 7]

Caffeic acid of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The term "Acetyl Mandelic Acid" of the present invention may specifically be "O-acetyl mandelic acid", the molecular formula is C ₁₀ H ₁₀ O ₄ , and the molecular weight is 194.186, It can be represented by Formula 8 below.

[Formula 8]

Acetyl mandelic acid of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The term "Laccaic Acid" of the present invention is one of natural anthraquinones, and is a dark red crystal prepared by condensing the secretions of Laccifer lacca Kerr , and is known to be mainly used as a dye. The laccasic acid is not particularly limited thereto, but may be laccasic acid A, B, C, D, E or F. In one embodiment of the present invention, Sigma-Aldrich's 50506 (Natural red 25) was used as the laccas acid.

The laccasan of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The term "ubiquinone" of the present invention is one of the fat-soluble quinones, and is a component of the mitochondrial electron transport chain. Specifically, the ubiquinone may be ubiquinone (10), but is not limited thereto. In this case, the molecular formula is C ₅₉ H ₉₀ O ₄ , the molecular weight is 863.37, and may be represented by Formula 9 below.

[Formula 9]

The ubiquinone of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The molecular formula of the term "Hydroxycinnamic Acid" of the present invention is C ₉ H ₈ O ₃ , the molecular weight is 164.158, and isomers (o-, m-, It may include both p-hydroxycinnamic acid) and isomers (cis-type coumaric acid and trans-type coumaric acid) by double bonds, and may specifically be m-Coumaric acid, It is not particularly limited thereto. The hydroxycinnamic acid may be represented by Formula 10 below.

[Formula 10]

The hydroxycinnamic acid of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The term "xanthophyll" of the present invention is a carotenoid-based oxycarotenoid pigment present in the green parts of plants, such as leaves, flowers, and fruits, and exhibits a yellow color. The molecular formula is C ₄₀ H ₅₆ O ₂ , and the molecular weight is 569. . The xanthophyll may be specifically lutein, and may be represented by Chemical Formula 11 below.

[Formula 11]

The xanthophyll or lutein of the present invention is not limited to its obtaining method, and may be chemically synthesized by a method known in the art or a commercially available material may be used.

The formononetin, emodine, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid or xanthophyll is in solvated form It may also exist in unsolvated form. In addition, the compounds may exist in crystalline or amorphous forms, and all such physical forms are included within the scope of the present invention.

In addition, it is apparent that the scope of the compounds includes not only the compounds but also cosmetically, food- and pharmaceutically-acceptable salts thereof.

The term " Magnoliae Cortex " of the present invention is a dried stem or root bark of Magnoliae family plant, and contains lignans and essential oil components as main active ingredients. It is known that back gourd has anti-inflammatory, analgesic, smooth muscle control, and anti-ulcer effects. The zucchini may be purchased commercially, or those harvested or cultivated in nature may be used without limitation.

The term " Aloe vera " of the present invention is a perennial plant belonging to the Liliaceae Aloe genus, and is also referred to as aloe vera. It contains aloin, aloeemodin, aloetin, and alomitin as major active ingredients, and is known for its antibacterial, antifungal, and antitumor effects. The aloe can be purchased commercially, or can be used without limitation when harvested or cultivated in nature.

The term "extract" of the present invention refers to a liquid component obtained by immersing a desired material in various solvents and then extracting at room temperature, low temperature or elevated temperature for a certain period of time, and a liquid component obtained by removing the solvent from the liquid component. It means the result of solid content, etc. In addition, in addition to the above results, it can be comprehensively interpreted as including all dilutions of the results, concentrates thereof, adjusted products, and purified products thereof.

In the present invention, the extract may be a silver leaf extract or an aloe extract. The extract may be extracted from various organs of natural, hybrid, and mutant plants, and may be extracted from, for example, roots, aerial parts, stems, leaves, flowers, fruit bodies, and fruit skins as well as plant tissues. The extract may be obtained by extraction with water or various organic solvents. At this time, the organic solvent used is not particularly limited thereto as long as an extract can be obtained, but specifically, it may be water, a polar solvent or a non-polar solvent, and more specifically, water, a lower alcohol having 1 to 6 carbon atoms ( methanol, ethanol, propanol or butanol, etc.), or a mixed solvent thereof.

In addition, the method for obtaining the extract is not particularly limited thereto, as long as the extract of the pumpkin or aloe can be obtained, but specifically, the roots, stems, leaves, fruits, flowers, and dried products of the pumpkin or aloe, Methods such as a cold needle extraction method in which a processed material is immersed in the solvent and extracted at room temperature, a heating extraction method, an ultrasonic extraction method in which ultrasonic waves are applied, and a reflux extraction method using a reflux condenser may be used.

The term "fraction" of the present invention means a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing various components. In the present invention, the fraction may be a fraction of a pumpkin extract or a fraction of an aloe extract.

In the present invention, the fractionation method for obtaining the fraction is not particularly limited as long as it can exhibit effects of increasing skin elasticity and reducing wrinkles, but may be performed according to a method commonly used in the art. For example, solvent fractionation performed by treating various solvents, ultrafiltration fractionation performed by passing through an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (separation according to size, charge, hydrophobicity or affinity) It may be a chromatographic fractionation method that performs), and a combination thereof, and the like.

In the present invention, the type of fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water, distilled water, and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohol is used among the fractionation solvents, C1 to C4 alcohols may be used.

The cosmetic composition of the present invention includes formononetin, emodine, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, It may contain 0.0005 to 10% by weight (w/w) of at least one material selected from the group consisting of topil, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract, based on the total weight of the composition. It may be included in 0.001 to 5% by weight (w / w), but is not limited thereto.

In the present invention, the cosmetic composition can promote the differentiation of human adipose derived stem cells into adipocytes. That is, the cosmetic composition may increase skin elasticity and improve wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes.

The term "human adipose derived stem cell" of the present invention refers to a stem cell that can be harvested from human adipose tissue and refers to a multipotent cell capable of differentiating into various cells of the body. Adipose-derived stem cells exist in subcutaneous adipose tissue and have the ability to differentiate into adipocytes. Adipokine secreted by adipose-derived stem cells is known to not only increase collagen synthesis in the dermal layer of the skin, but also affect skin regeneration.

The term "adipose cell" of the present invention refers to cells that store fat in the adipose tissue of an individual, and may specifically mean white adipocytes present in the subcutaneous fat layer, but is not limited thereto. don't

In one embodiment of the present invention, formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone are added to human adipose-derived stem cells. , As a result of treatment with hydroxycinnamic acid, xanthophyll, zucchini extract, and aloe extract, it was confirmed that the differentiation of human adipose-derived stem cells into adipocytes could be effectively promoted. Therefore, the composition of the present invention containing the above substances can be used for anti-aging purposes by increasing skin elasticity and improving wrinkles.

The term "increasing skin elasticity" of the present invention means enhancing skin elasticity by strengthening the subcutaneous fat layer structure of an object using the composition of the present invention. As long as the skin can be increased in elasticity or improved in wrinkles with the composition of the present invention, the location of the skin of the subject may be included without limitation.

The term "improvement" of the present invention means alleviating the symptoms of skin wrinkles of a subject by using the composition of the present invention.

In one embodiment of the present invention, the skin may be lip skin, but is not limited thereto. The active ingredient of the cosmetic composition for increasing the elasticity of the lip skin and improving wrinkles may be one or more substances selected from the group consisting of extracts of pumpkin extracts and fractions of extracts of pumpkin extracts, but is not limited thereto.

Unlike other parts of the skin, the lips do not have sebaceous glands and have a very thin stratum corneum. Currently, most of the volume and wrinkle improving ingredients used for lips are applied as ingredients to improve the dermal layer of the skin, and the subcutaneous fat layer improvement technology, which occupies the largest volume in the skin, has limitations that cannot be actively applied. In addition, considering that the stratum corneum layer of the lips is very thin, penetration and delivery of active ingredients will be easier than other skin regions, the technology for improving the volume of the subcutaneous fat layer can be expected to be a more effective technology in terms of volume and wrinkle improvement. The composition of the present invention is characterized by its excellent effect on the skin of the lips. In one embodiment of the present invention, it was confirmed that the wrinkle improvement of the extract of japonica extract has excellent effects on both the eyes and lips, and it was confirmed that the improvement rate and rate of improvement were higher in the lips than in the eyes.

The cosmetic composition for the lip skin may be prepared in a formulation selected from the group consisting of lipstick, lip gloss, lip tint, and lip balm, but is not limited thereto.

In the present invention, the cosmetic composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath additive, sunscreen cream, sun oil, Suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays may be prepared in formulations selected from the group consisting of It is not limited.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as typical ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, A thickener, a chelating agent, a colorant, a preservative, a flavoring agent, and the like may be suitably blended, but are not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation.

When the dosage form of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in particular, in the case of a spray, chloro propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan may be used. there is.

In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohols, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier components. there is.

The cosmetic composition of the present invention includes formononetin, emodine, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, xanthophyll , Sulfur extract, aloe extract, fractions of zucchini extract, and at least one material selected from the group consisting of fractions of aloe extract, commonly used adjuvants such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic actives, preservatives, antioxidants , solvents, fragrances, fillers, blockers, pigments, odorants and dyes, and the like.

Another aspect of the present invention is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, Provided is a food composition for increasing skin elasticity and improving wrinkles, comprising at least one material selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract as an active ingredient. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes.

The formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laconic acid, ubiquinone, hydroxycinnamic acid, xanthophyll, saffron, aloe, Extracts, fractions, increase and improvement of skin elasticity are as described above.

In one embodiment of the present invention, the skin may be lip skin, but is not limited thereto. The active ingredient of the food composition for increasing the elasticity of the lip skin and improving wrinkles may be one or more substances selected from the group consisting of extracts of pumpkin extracts and fractions of extracts of pumpkin extracts, but is not limited thereto.

In addition, the present invention is formononetin, emodine, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid or xanthophyll. It provides a food composition for increasing skin elasticity and improving wrinkles, including a food-acceptable salt as an active ingredient.

In the present invention, "food acceptable salt" is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone , It is in the form of a salt in which hydroxycinnamic acid or xanthophyll is combined with other substances, and means a substance that can exhibit an activity similar to that of the compound in terms of food science.

The food composition of the present invention contains formononetin, emodin, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, xanthophyll , Sulfur extract, aloe extract, fractions of Sculpture extract, and fractions of Aloe extract, in addition to at least one material selected from the group consisting of food additives acceptable in food science.

In the present invention, "food supplement additive" refers to a component that can be added to food supplementally, and is added to prepare a health functional food of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners , pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like, but are not limited thereto.

The functional food composition of the present invention may include health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, “functionality” means obtaining useful effects for health purposes, such as adjusting nutrients for the structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of dosage forms, and unlike general medicines, it has the advantage of not having side effects that can occur when taking medicines for a long time by using food as a raw material, and has excellent portability, so that the composition of the present invention Health functional food can be consumed as a supplement to increase skin elasticity and improve wrinkles.

There is no limit to the form that the health functional food of the present invention can take, and it can include all foods in a conventional sense, and can be used interchangeably with terms known in the art, such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing suitable other auxiliary ingredients and known additives that can be included in food according to the selection of those skilled in the art. Examples of food that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and Vitamin complexes, etc. It also includes food used as feed for animals.

Another aspect of the present invention is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, Provided is a pharmaceutical composition for increasing skin elasticity and improving wrinkles, comprising at least one substance selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract as an active ingredient. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes. The formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laconic acid, ubiquinone, hydroxycinnamic acid, xanthophyll, saffron, aloe, Extracts, fractions, increase and improvement of skin elasticity are as described above.

In one embodiment of the present invention, the skin may be lip skin, but is not limited thereto. The active ingredient of the pharmaceutical composition for increasing the elasticity of the lip skin and improving wrinkles may be one or more substances selected from the group consisting of extracts of Sperm exfolia, and fractions of extracts of Sperm exfolia, but is not limited thereto.

In addition, the present invention is formononetin, emodine, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid or xanthophyll. It provides a pharmaceutical composition for increasing skin elasticity and improving wrinkles, comprising a pharmaceutically acceptable salt as an active ingredient.

In the present invention, "pharmaceutically acceptable salts" are formononetin, emodine, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone , It is in the form of a salt in which hydroxycinnamic acid or xanthophyll is combined with another substance, and means a substance that can exhibit similar activity to the above compound pharmaceutically.

The pharmaceutical composition of the present invention can be used as a single formulation, and can be prepared and used as a combination formulation by further including a drug known to have an effect of increasing skin elasticity and improving wrinkles, and is formulated using a pharmaceutically acceptable carrier or excipient. It can be prepared in unit dose form or introduced into a multi-dose container.

The pharmaceutical composition may be administered in a pharmaceutically effective amount. The "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is dependent on the type and severity of the subject, age, sex, drug activity, and drug sensitivity, time of administration, route of administration and excretion rate, duration of treatment, factors including concomitantly used drugs, and other factors well known in the medical arts.

Another aspect of the present invention is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, Provided is a quasi-drug composition for increasing skin elasticity and improving wrinkles, comprising at least one material selected from the group consisting of xanthophyll, silver leaf extract, aloe extract, fractions of silver leaf extract, and fractions of aloe extract as an active ingredient. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes. The formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laconic acid, ubiquinone, hydroxycinnamic acid, xanthophyll, saffron, aloe, Extracts, fractions, increase and improvement of skin elasticity are as described above.

In one embodiment of the present invention, the skin may be lip skin, but is not limited thereto. The active ingredient of the quasi-drug composition for increasing the elasticity of the lip skin and improving wrinkles may be at least one substance selected from the group consisting of extracts of silver leaf extracts and fractions of extracts of silver leaf extracts, but is not limited thereto.

In addition, the present invention is formononetin, emodine, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid or xanthophyll. It provides a quasi-drug composition for increasing skin elasticity and improving wrinkles, containing a pharmaceutically acceptable salt as an active ingredient. The "pharmaceutically acceptable salt" is as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, aromatics, preservatives, and the like. can include

The "pharmaceutically acceptable carrier" may refer to a carrier, excipient, or diluent that does not inhibit the biological activity and properties of the compound to be injected without irritating the organism, and specifically, a non-naturally occurring carrier (non-naturally occurring carrier). occurring carrier). The type of the carrier that can be used in the present invention is not particularly limited, and any carrier commonly used in the art and pharmaceutically acceptable can be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and the like. These may be used alone or in combination of two or more.

The composition containing a pharmaceutically acceptable carrier may be in various oral or parenteral formulations. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the compound, for example, starch, calcium carbonate, sucrose, lactose, It may be prepared by mixing gelatin or the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid formulations for oral use include suspensions, solutions for internal use, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. there is. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, Witepsol, Macrogol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The quasi-drug composition of the present invention may include, but is not limited to, a disinfectant cleanser, shower foam, ointment, wet tissue, coating agent, etc. It can be appropriately selected from the description of.

In addition, the quasi-drug composition of the present invention may be used in a skin wrinkle improvement method comprising applying to the skin of a subject. The subject includes, without limitation, mammals including mice, livestock, humans, and the like.

Another aspect of the present invention is formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid Provided is a composition for promoting differentiation of human adipose-derived stem cells, comprising, as an active ingredient, one or more substances selected from the group consisting of , xanthophylls, Sulfur extracts, aloe extracts, fractions of Sculpture extracts, and fractions of Aloe extracts. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes. The formononetin, emodin, artefillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laconic acid, ubiquinone, hydroxycinnamic acid, xanthophyll, succulent, aloe, Extracts, fractions, increase and improvement of skin elasticity are as described above.

Another aspect of the present invention provides a method for increasing skin elasticity, comprising administering the composition to a non-human subject.

As used herein, the term "administration" means introducing the composition of the present invention to a patient by any suitable method, and the administration route of the composition can be administered through any general route as long as it can reach the target tissue. there is. Intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration may be administered, but is not limited thereto. Since the composition of the present invention is effective in improving skin elasticity and improving wrinkles, the administration route of the composition may be administered by applying it to the skin.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into 2 to 3 times. In addition, the composition of the present invention can be used alone or in combination with other drug treatments to improve skin elasticity and wrinkles. It is important to administer the amount that can obtain the maximum effect with the minimum amount without side effects in consideration of all the above factors, and can be easily determined by those skilled in the art.

As used herein, the term "subject" refers to all animals such as rats, mice, livestock, etc., including humans, in which skin elasticity is reduced or skin wrinkles are or may occur. Specifically, it may be mammals including humans.

The composition of the present invention can be used for anti-aging purposes by promoting the differentiation of human adipose-derived stem cells into adipocytes, thereby strengthening the subcutaneous fat layer structure, increasing skin elasticity, and improving wrinkles.

1 shows the results of staining lipids in differentiated adipocytes with Oil Red O after treating human adipose-derived stem cells with formononetin, acetyl mandelic acid, and humpback extract.
Figure 2 is formononetin, emodin, artepillin, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, lacric acid, ubiquinone, hydroxycinnamic acid, xanthophyll, silver leaf extract and relative differentiation rates of human adipose-derived stem cells treated with the aloe extract were quantitatively shown.
Figure 3 shows the results of comparing people with improved wrinkles around the eyes and lips of the extract of Hurbana.
Figure 4a shows the persistence of improvement in lip wrinkles until the 12th week of subject 1 when treated with the henna extract, and Figure 4b shows the persistence of improvement in lip wrinkles until the 12th week of subject 2.
Figure 5 shows the results of comparing the improvement rates of wrinkles around the eyes and lips of asiaticoside, which is a conventional wrinkle-improving material, and extracts of black pepper.

Hereinafter, the present invention will be described in detail by means of the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Example 1: Preparation of zucchini and aloe extracts

The extract of this embodiment was purchased from Borak Co., Ltd. (Hwaseong, Gyeonggi-do, Korea), and the powder of Magnoliae Cortex was dissolved in a solvent such as DPG or DMSO, and then filtered with a 0.2 μm syringe filter. did

To prepare the aloe extract of this example, Aloe Vera was purchased from a domestic farm and extracted once at 70° C. at a ratio of 1:10 using 70% methanol.

Example 2 Confirmation of Formononetin, Acetyl Mandelic Acid, and Sulfur Extract for Promoting Differentiation of Human Adipose-Derived Stem Cells

Human adipose derived stem cells were purchased from Lonza Inc (Walkersville, MD, USA) and cultured according to Lonza's experimental guidelines. Differentiation into adipocytes was tested using a differentiation medium recommended by Lonza. As a positive control, 1uM of Rosiglitazone was used and treated as a differentiation medium. In order to induce the differentiation of adipose-derived stem cells into adipocytes, after 3 to 5 days from the start of culturing the cells, the cell culture medium was removed and formononetin, acetyl mandelic acid, extracts of silver ginseng, and a positive control were treated for 14 days.

14 days after the start of differentiation into adipocytes, the cell culture medium was removed, the cells were washed with phosphate buffer (DPBS), and then the cells were fixed using 10% formalin. The fixed cells were observed under a microscope by staining lipids in differentiated adipocytes with Oil Red O. As a result, treatment with representative substances such as Formononetin, Acetyl Mandelic acid, and Succulent extract showed a higher differentiation effect of adipose-derived stem cells into adipocytes compared to treatment with only the differentiation medium (negative control). It can be seen that it is excellent (Fig. 1).

Example 3: Quantitative confirmation of the differentiation promoting effect of human adipose-derived stem cells of 12 compounds including formononetin, zucchini extract and aloe extract

In the same manner as in Example 2, after treating cultured human adipose-derived stem cells with 12 compounds including formononetin, zucchini extract and aloe extract, and then eluting the stained Oil Red O with 100% isopropanol solution, 490 nm The absorbance was measured and quantified at the wavelength, and the results are shown in FIG. 2 . From this, each of the 14 materials including formononetin of the present invention showed a significant increase in the relative differentiation rate compared to the negative control group treated with only the differentiation medium, promoting the differentiation of adipose-derived stem cells into adipocytes, ), it was confirmed that differentiation into adipocytes can be effectively promoted compared to non-differentiating materials such as. Therefore, the composition of the present invention containing the above substances can be used for anti-aging purposes of increasing skin elasticity and improving wrinkles by promoting differentiation of adipose-derived stem cells and increasing subcutaneous adipocytes.

Example 4: Confirmation of the effect of improving the lip skin wrinkles of silver gourd extract

In order to confirm the effect of improving skin wrinkles of the pumpkin extract prepared in Example 1, the subjects (total number of subjects: 12) were treated with the extract of the extract on the lips and wrinkles of the skin under the eyes.

The wrinkles of the lip skin were measured by designating the most protruding part of the lower lip by a certain length through Janus photography and obtaining the standard deviation of the contrast value. The range of standard deviation of the contrast value was 3 to 12, and it was confirmed that the standard deviation was larger as the number of deep wrinkles increased. The wrinkles of the skin under the eyes were measured by taking a picture of Janus and calculating the area of wrinkles in Janus with a wrinkle analysis program. It was confirmed that the wrinkle values ranged from 7 to 44.

Results in which the change in the lip wrinkle value was 1 or more and the change in the wrinkle value under the eyes was 4 or more were classified as improved result values. This classification criterion confirmed that the numerical values that can clearly distinguish improvement with the naked eye were 2 for lip wrinkles and 8 for wrinkles under the eyes, and 1/2 of these values was selected as the criterion for determining whether or not to improve.

As a result of treating the skin of the lips with the extract of japonica extract, improvement started from the 4th week, and it was confirmed that the number of improved subjects (8) was the maximum at the 8th week, and similarly maintained until the 12th week. As a result of treating the wrinkles under the eyes with the extract of the extract, it was improved at a rate similar to that of the lip skin by the 4th week, and it was confirmed that the number of subjects who improved by the 12th week increased, although at a low rate of increase (FIG. 3).

In addition, it was confirmed that the wrinkle improvement effect was maintained and the elasticity of the lip skin was improved until the 12th week after the extract of the extract was applied to the subject's lip skin (FIG. 4). This is the result of confirming that the extract of sagebrush promotes the differentiation of adipose stem cells and increases subcutaneous fat cells, thereby actually improving wrinkles and elasticity of the skin.

As a result of comparing the wrinkle-improving effects of asiaticoside, a conventional wrinkle-improving material, on the skin of the lips and the skin under the eyes, the extract of asiaticoside was superior to the skin under the eyes in the rate and rate of improvement in wrinkles of the lip skin, and asiaticoside was superior to the skin of the lips. It was confirmed that the improvement rate and rate of improvement in the wrinkles of the skin under the eyes were excellent (FIG. 5).

Through this, it can be seen that the pumpkin extract has an effect of improving skin wrinkles, and is particularly effective in improving wrinkles of the lip skin.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (20)

A cosmetic composition for increasing skin elasticity and improving wrinkles, comprising palmitamide as an active ingredient.
The cosmetic composition according to claim 1, wherein the composition increases skin elasticity and improves wrinkles by promoting differentiation into adipocytes.
The cosmetic composition according to claim 1, wherein the skin is lip skin.
The cosmetic composition according to claim 3, wherein the cosmetic composition for lip skin is a formulation selected from lipstick, lip gloss, lip tint, or lip balm.
The cosmetic composition according to claim 1, comprising a cosmetically acceptable salt of palmitamide as an active ingredient.
The cosmetic composition according to claim 1, wherein the active ingredient is included in an amount of 0.0005 to 10% by weight (w/w) based on the total weight of the composition.
delete The cosmetic composition according to claim 1, wherein the composition promotes the differentiation of human adipose derived stem cells into adipocytes.
◈Claim 9 was abandoned when the registration fee was paid.◈ According to claim 1, the composition softening lotion (nutritional emulsion), astringent lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, skin bonding patch, skin bonding gel, powder, ointment, suspension, emulsion, A cosmetic composition that is a formulation selected from sprays or cosmetic liquids.
A food composition for increasing skin elasticity and improving wrinkles, comprising palmitamide as an active ingredient.
The food composition according to claim 10, wherein the composition increases skin elasticity and improves wrinkles by promoting differentiation into adipocytes.
The food composition according to claim 10, wherein the skin is lip skin.
11. The food composition according to claim 10, comprising a pharmaceutically acceptable salt of palmitamide as an active ingredient.
A quasi-drug composition for increasing skin elasticity and improving wrinkles, comprising palmitamide as an active ingredient.
The quasi-drug composition according to claim 14, wherein the composition increases skin elasticity and improves wrinkles by promoting differentiation into adipocytes.
The quasi-drug composition for increasing skin elasticity and improving wrinkles according to claim 14, wherein the skin is lip skin.
The quasi-drug composition according to claim 14, comprising a pharmaceutically acceptable salt of palmitamide as an active ingredient.
A composition for promoting the differentiation of human adipose-derived stem cells into adipocytes, comprising palmitamide as an active ingredient.
The composition according to claim 18, wherein the composition increases skin elasticity and improves wrinkles by promoting differentiation into adipocytes.
A method of increasing skin elasticity, comprising administering a composition containing palmitamide as an active ingredient to a non-human subject.

Images