KR20170141564A - Cosmetic composition containing propolis and extracts of opuntia humifusa - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a propolis extract and an Eastern prickly pear extract as active ingredients. The propolis extract and the Eastern prickly pear extract of the present invention are derived from natural substances such as edible plants, and also ensures moisturizing functions as well as antibacterial functions required in existing soap compositions, with little issues associated with side effects or safety, thereby making up shortcomings that existing soap products have had. Accordingly, the cosmetic composition can be widely used in cosmetic products for various purposes, especially for cleansing.

Description

COSMETIC COMPOSITION CONTAINING PROPOLIS AND EXTRACTS OF OPUNTIA HUMIFUSA <br> <br> <br> Patents - stay tuned to the technology COSMETIC COMPOSITION CONTAINING PROPOLIS AND EXTRACTS OF OPUNTIA HUMIFUSA

The present invention relates to a cosmetic composition comprising a natural component having an antibacterial effect and a moisturizing effect as an active ingredient, specifically a soap composition.

The human skin becomes sensitive due to changes in the environment, stress due to internal factors, and external stimuli, and water retention may be decreased. In such a case, various skin troubles may occur.

Particularly, when the moisture holding power of the skin is lowered in relation to the progress of aging, the skin becomes dry, the elasticity is lowered, the wrinkle is increased, and the skin color may change to a dull color.

In addition, when the moisture retention of the skin is lowered, it is easily susceptible to external weak stimuli and takes a long time to recover. In addition, the most important role of the skin in terms of the skin is to prevent the loss of biocomponents such as water and electrolytes by barrier function, and to prevent harmful substances of the external environment from penetrating into the skin.

That is, the skin plays an important role as a protective film protecting the living body from the external environment while being exposed to a much drier external environment than the living body. Particularly, the outermost layer of the skin, which is in direct contact with the external environment, prevents physical and chemical harmful components from entering, and prevents internal moisture loss to the external environment that is drier than in vivo. These stratum corneum has various functions to maintain flexibility by holding proper moisture inside the stratum corneum.

More specifically, it is generally known that the skin moisture content is about 70% in the dermis, and decreases to the epidermis and is about 10% to 30% in the stratum corneum, which is the outermost layer. The water supplied from the dermis diffuses mainly to the upper part of the stratum corneum by passive diffusion, and the moisture diffused to the upper part of the stratum corneum is lost to the outside. This is called TEWL (Trans Epidermal Water Loss), and it is known that the lipid component of the stratum corneum and epidermal lipid, which prevents this transdermal water loss,

Therefore, the water retention ability of the stratum corneum is not only important from the viewpoint of biological defense, but also smooth and flexible skin surface is very important from a cosmetic point of view. However, in spite of the role of the skin, especially the skin's stratum corneum, the external stimuli such as wind, cold weather, sunlight, external stimuli such as cleansing, shaving, Moisture can be lost and the skin becomes dry due to moisture loss.

Therefore, a lot of efforts have been made to solve moisture loss of the skin which causes skin troubles. Typical methods are to use cosmetics containing natural vegetable ingredients and stimulants to block the moisture loss of the skin Have been proposed.

In the past, cosmetics, which were thought to be products used by women, are now used not only as a means of expressing beauty but also as daily necessities, regardless of whether they are male or female. As the industry develops, As a result, there is a growing interest in 'functional' products that expect special effects and efficacy.

Functional cosmetics currently recognized in Korea are whitening products that help to whiten skin, skin moisturizing products that help skin moisturize the skin, ultraviolet shielding products that help protect skin from UV rays, have.

Generally, these functional cosmetics are interpreted as cosmetics that affect not only the cosmetic effect but also the structure and function of the body, and prevent and treat the cosmetics. In recent years, sales of functional cosmetics have increased steadily in total sales of cosmetics, and research has been actively conducted to find new effective ingredients for the human body and to utilize them effectively to the skin.

Recently, there has been limited research on the most important function of cosmetics to maintain skin smooth and moist by blocking moisture loss of skin in recent years. Specifically, cosmetics make an artificial membrane on the skin to prevent external stimulation, prevent water loss from the inside of the skin, and supply a certain amount of moisture to the skin to maintain a smooth and moist skin.

However, conventional moisturizing agents such as water-soluble polyhydric alcohols, sodium pyrrolidonecarboxylate, urea, amino acids, and elastin, which are used as effective ingredients in cosmetics for moisturizing, have problems.

For example, in relation to water-soluble polyhydric alcohols most commonly used as moisturizing agents in cosmetics, glycerin and solitol having three or more OH groups have a problem of being uncomfortable when they are used because they are highly sticky to moisture. In addition, sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate or urea, which is a natural moisturizing factor (NMF), has a problem of deteriorating the emulsion stability of products such as cosmetics There are amino acids, collagen, elastin, etc., but it has a limited ability to moisturize.

Recently, recent researches related to functional materials have been focused on natural materials derived mainly from natural materials in conjunction with environmentally friendly hot air. However, these studies are focused on the part related to the above-mentioned functional cosmetics, but they are limited in terms of the moisturizing effect.

In addition, a typical soap as a cosmetic product for cleaning is one for cleansing and protecting the skin. Therefore, most of the soaps are required to have a cleaning power capable of removing stains adhering to the human body and a bubble power capable of softening a feeling of use, but recently, it is required to have a moisturizing function as well as a washing power and an antibacterial power, have.

Specifically, the soap should have antiseptic and antimicrobial effects so as to prevent malodorous and bacterial infections caused by various bacteria and fungi that parasitize human skin. In addition, as the cleansing function is maximized in order to help the cleansing and skin health of the skin, the sebum components accumulated in the epithelium of the skin are excessively removed, thereby causing skin roughness or dryness, It has been required to have an effect of imparting a moisturizing function to the skin.

KR 2012-0000251 A KR 10-1041444 B KR 10-0948517 B KR 10-0910747 B KR 10-1183484 B

In order to meet such a demand, the object of the present invention is to provide a cosmetic composition having an antibacterial function and a moisturizing function using an effective ingredient of a plant extract which is less likely to cause side effects. The cosmetic composition may be a soap composition.

In order to achieve the above object, the present invention the propolis extract and cheonnyeoncho extract (Opuntia humifusa ) as an active ingredient.

The propolis extract is prepared by drying a cap bristle followed by pulverization to 200 mesh or less to prepare a cap bristle powder; 500 to 2,000 parts by weight of a 50% to 90% ethanol aqueous solution based on 100 parts by weight of the glue powder is added to the glue powder, and the mixture is continuously stirred and stirred at 10 ° C to 20 ° C for 10 hours to 30 hours to prepare an extract. Manufacturing steps; A fermentation broth fermentation broth for fermenting the fermented broth extract at 20 ° C to 40 ° C for 20 days to 45 days, followed by filtration to produce a fermented broth fermentation broth; And a step of preparing propolis extract by stirring the fermented broccoli fermentation broth at 60 ° C to 80 ° C for 10 days to 20 days every time with stirring to prepare a propolis extract.

The above-mentioned Citrus Seed Extract may be obtained by solvent extraction with an aqueous 60% ethanol solution or ethanol.

The content of the propolis extract may be 0.1% (w / w) to 5% (w / w), specifically 1% (w / w) based on the total weight of the cosmetic composition.

The content of the citrus extract may be 0.1% (w / w) to 5% (w / w), specifically 1% (w / w) based on the total weight of the cosmetic composition.

The cosmetic composition may be an antibacterial soap composition having a moisturizing activity.

The cosmetic composition may further comprise an albumen extract.

The inventors of the present invention have conducted research on natural products having an antibacterial effect and moisturizing effect and found that the propolis extract prepared by the preparation method of the present invention has an excellent antibacterial effect as compared with the conventional propolis, The present invention has been completed based on the fact that the quinine-annual extract has the best moisturizing effect.

In the present invention, the effective component means a component of a pharmaceutical composition or cosmetic composition which provides a anti-inflammatory effect.

As used herein, the term &quot; extract &quot; means an extract obtained by treating an extractive solvent with a plant or a product prepared by the method of producing an extract, unless otherwise specified. The extract may be formulated ) Results.

The extract has the meaning of being used as a crude extract, but broadly includes fractions obtained by further fractionating the extract. Specifically, the above-mentioned extract includes not only an extract obtained by using an extraction solvent, but also an extract obtained by additionally applying a purification process thereto. For example, the extract may contain a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, a fraction obtained by various chromatographies (prepared for separation according to size, charge, hydrophobicity, or affinity) Further comprising fractions obtained through various purification methods.

In this specification, unless otherwise indicated, administration means to provide any desired composition of the invention to the subject by any suitable method.

As used herein, unless otherwise specified, an individual refers to any animal, particularly a mammal, including humans, monkeys, dogs, goats, pigs or rats, who have undergone female polyneuropathy syndrome by administration of the composition of the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention relates to an extract of Propolis and Opuntia sp. humifusa extract as an active ingredient.

Propolis is a natural product called russian penicillin or natural penicillin. It is a substance made by mixing bees with their own needles and enzymes in a resin-like substance extracted from various plants for their survival and reproduction. The propolis has been known to have anti-inflammatory and antioxidant effects.

The propolis extract is prepared by drying a cap bristle followed by pulverization to 200 mesh or less to prepare a cap bristle powder; 500 to 2,000 parts by weight of a 50% to 90% ethanol aqueous solution based on 100 parts by weight of the glue powder is added to the glue powder, and the mixture is continuously stirred and stirred at 10 ° C to 20 ° C for 10 hours to 30 hours to prepare an extract. Manufacturing steps; A fermentation broth fermentation broth for fermenting the fermented broth extract at 20 ° C to 40 ° C for 20 days to 45 days, followed by filtration to produce a fermented broth fermentation broth; And a step of preparing a propolis extract by stirring the fermented broccoli fermentation broth at 60 ° C to 80 ° C for 10 days to 20 days every time with stirring to prepare a propolis extract.

The above-mentioned Opuntia Humifusa , Eastern prickly pear) is a plant belonging to the cactus and fern cactus, characterized by the stem being flat like a palm. The cheonnyeoncho is baeknyeoncho the tropics grown in Jeju, known as your plants originating (Opuntia ficus - indica ), it is a native species of Korea and has a characteristic of growing to about 30 to 40 cm in soil with a fluffy thorn, usually sowing in May, blooming in June, and harvesting in October to November. It is a Korean native palm cactus with a cold tolerance enough to survive even at a cold temperature of minus 20 ℃. It has been known from the past that the tsunami seeds have been effective for the treatment of constipation, diuretic, bowel movements, inflammation and arthritis, and recent studies have revealed that they have various physiological activities such as antioxidation, antiinflammation, It is getting higher.

Since the fermented product of the perennial plant extract is derived from a natural substance, it has no side effects, has no cytotoxicity, has excellent moisturizing effect, and the propolis extract is derived from a natural substance used as a food, And the antibacterial effect is excellent. Thus, the propolis extract and the Chenille seed extract can be used as an effective ingredient of a cosmetic composition having a moisturizing effect and an antibacterial effect.

The quinterniaceae extract may be one prepared by a conventional method for producing plant extracts. For example, the quinterniaceae extract may be prepared by extracting an extract solvent with the extract of the plant, or a dried product thereof, a pulverized product thereof or a juice thereof, And then fractionating the extract with a fraction solvent.

The above millennia are not limited to harvested, cultivated or marketed, and the millennia may be used at any stage during the growing stage.

The method for preparing the above extract can be produced by a conventional method for producing a plant extract, specifically a solvent extraction method, a supercritical extraction method, a subcritical extraction method, a high temperature extraction method, a high pressure extraction method or an ultrasonic extraction method, Resin. &Lt; / RTI &gt; The adsorption resin may be, for example, XAD or HP-20. The method for preparing the extract may be, for example, a solvent extraction method.

The solvent extraction method may be a heat extraction method, a cold extraction method, a warm-up extraction method, or the like, and a conventional extraction device, an ultrasonic pulverizing extractor, a supercritical extractor, a subcritical extractor or a fractionator may be used.

The extracting solvent used for the solvent extraction may be any one selected from the group consisting of water, an organic solvent, and a combination thereof. The organic solvent may be selected from the group consisting of 1 to 5 carbon atoms A polar solvent such as alcohol, diluted alcohol, ethylacetate or acetone, and a non-polar solvent such as ether, chloroform, benzene, hexane or dichloromethane, or a mixed solvent thereof. The alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, isopropanol and the like, but is not limited thereto. In addition, the alcohol diluted water may be diluted with water to 60% (v / v) to 99.9% (v / v) alcohol.

The extraction solvent of the tsunami extract of the present invention is preferably at least one selected from the group consisting of water, an alcohol having 1 to 5 carbon atoms, an alcohol-diluted water, and a mixture thereof, more preferably 60% (v / v ) To 99.9% (v / v) aqueous solution of ethanol or 100% (v / v) ethanol, and still more preferably ethanol.

The extraction process may be performed at, for example, 50 ° C to 150 ° C, or 75 ° C to 120 ° C, or 80 ° C to 100 ° C, but is not limited thereto. The extraction time is not particularly limited, but may be 10 minutes to 24 hours, or 30 minutes to 12 hours, or 2 hours to 5 hours.

The extract may be a fraction prepared by extracting with an extraction solvent or extracting with an extraction solvent and fractionating the extract with a fraction solvent.

The fraction is extracted with an extraction solvent and extracted with any one solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof, preferably hexane, methylene chloride and ethyl acetate And the fractionation process is further carried out with at least one solvent selected from the group consisting of Two or more kinds of solvents may be used for the fractionation, and fractions of each extract may be prepared by sequentially using them or by mixing them according to the polarity of the solvent.

The fractionation process may be performed, for example, at 4 ° C to 120 ° C, or 50 ° C to 90 ° C, or 60 ° C to 80 ° C, but is not limited thereto.

The slug extract or the fraction obtained by performing the fractionation process may be subjected to filtration, concentration or drying to remove the solvent, followed by filtration, concentration, and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a vacuum concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying.

As an example of the Chenille seed extract, Chenille seeds may be put into an extraction vessel together with an extraction solvent and extracted and filtered, followed by concentration under reduced pressure, followed by freeze-drying. The extracts obtained can be stored in a deep freezer until use.

The content of the propolis extract in the cosmetic composition may be 0.1% (w / w) to 5% (w / w) or 1% (w / w) based on the total weight of the cosmetic composition, (W / w) to 5% (w / w) or 1% (w / w) based on the total weight of the cosmetic composition.

The cosmetic composition may be used for a soap composition, a foundation cosmetic, a makeup cosmetic, a body cosmetic, a hair cosmetic, a scalp cosmetic, a shaving cosmetic or an oral cosmetic composition, have.

The soap composition may be an antibacterial soap composition having a moisturizing activity.

Examples of the cosmetic cosmetic include a cream, a lotion, a pack, a massage cream, and a milky lotion. Examples of the cosmetic cosmetic include a foundation, a makeup base, a lipstick, an eye shadow, an eyeliner, a mascara, an eyebrow pencil, Examples of the cosmetic material include soap, liquid cleansing agent, bathing agent, sunscreen cream, and sun oil. Examples of the cosmetic for hair include shampoo, rinse, hair treatment, hair mousse, hair liquid, pomade, hair color, hair bleach Examples of the cosmetic composition for scalp include hair tonic or scarf treatment, and examples of the cosmetic composition for shaving include aftershave lotion or shaving cream. Examples of the oral cosmetic composition include Toothpaste or mouth washer.

The cosmetic composition may contain, in addition to the active ingredient, a component incorporated in the cosmetic composition, for example, a moisturizer, an ultraviolet absorber, a vitamin, an animal or plant extract, a digestive agent, a whitening agent, a vasodilator, Hormones and the like may be further added. In addition, the cosmetic composition may further include a medicament or a mechanism necessary for infiltrating or transferring the active ingredient into the dermal tissue.

The formulation of the cosmetic composition may take any appropriate form depending on the intended use and the properties of the cosmetic composition. Examples of the formulation include an aqueous solution, a solubilization system, an emulsion system, an emulsion system, a gel system, a paste system, an ointment system, A two-layer system or a water-oil-powder three-layer system. Examples of the formulations are merely illustrative, and the formulations and forms of the cosmetic composition of the present invention are not limited by the above examples.

The active ingredient may be used in an amount of 0.001 to 5% by weight or 0.01 to 2% by weight based on the total weight of the cosmetic composition. The content of the active ingredient may be appropriately adjusted depending on the content of the ingredient other than the active ingredient contained in the formulation or cosmetic composition And the content of the active ingredient contained in the present invention is not limited by the above content.

The soap composition may contain a soap chip or a soap base and an active ingredient propolis extract and a water-soluble antioxidant extract, and may further contain an antibacterial component, a moisturizing ingredient or a perfume.

The antimicrobial component may be a whitening extract.

The moisturizing component may be oil, honey or glycerin.

The fragrance may be lavender, peppermint, rosemary, tea tree, lemon, chamomile or blueberry flavor.

The soap composition may be mixed with various other raw materials such as soda according to a conventional soap manufacturing method, and then molded and extruded into a conventional soap making apparatus to be made into a soap.

The propolis extract of the present invention and the tsunami extract of the present invention are natural products such as plants used for edible use and have little problems about side effects and safety and have been given not only the antibacterial activity required for existing soap compositions but also the moisturizing function, The present invention can be widely used in cosmetics for various purposes, particularly in cosmetics for cleaning purposes.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph of a mushroom extract prepared using an ethanol or ethanol aqueous solution according to an embodiment of the present invention. FIG.
FIG. 2 is a photograph of a soap prepared according to an embodiment of the present invention. FIG. 2 is a soap prepared by adding 10% (w / w) of an upper left soap and 5% (w / w) And the bottom left is a soap added with 1% (w / w), and a soap with 0.5% (w / w) added thereto.
FIG. 3 shows antibacterial effect of soap according to an addition amount of propolis extract according to an embodiment of the present invention, wherein Control means soap not containing propolis extract and% concentration at the bottom of photographed image indicates propolis extract Means the addition amount, specifically, the addition amount (%) of propolis extract to the total weight of the soap composition (w / w).
FIG. 4 shows antibacterial effect of soap according to one embodiment of the present invention, wherein C (control) means a soap without added propolis extract and Chenille seed extract, and 50% is 50% (W / w) is added to the total weight of the soap composition, and 100% of the 100% ethanol extract is added to the soap composition in an amount of 1% (w / w) (w / w) is added to the total amount of the soap, and 0.5% of the propolis extract is added to the total composition weight of the soap (W / w), and 30 min means that the water content was measured after 30 minutes of washing.

Hereinafter, the structure and effects of the present invention will be described in more detail with reference to specific examples and comparative examples in order to facilitate understanding of the present invention. However, it should be understood that the following examples are for the purpose of further illustrating the present invention and are not to be construed as limiting the scope of the invention as defined by the appended claims. All technical ideas are to be construed as falling within the scope of the present invention.

< Manufacturing example  1> Millennium  Preparation of Extracts and Propolis Extracts

The cheonnyeoncho used in this experiment (Opuntia humifusa ) was used to provide the perennial trunks grown directly by Jeollanam - do plantation in Jeollanam - do.

The samples of the millennium were extracted with a solvent extraction method using an ethanol or ethanol aqueous solution to prepare extract samples.

200 mL of 20% ethanol aqueous solution, 200 mL of 40% ethanol aqueous solution, 200 mL of 60% ethanol aqueous solution, and 80 mL of a 60% ethanol aqueous solution were added to 50 g of the three- 200 mL of ethanol aqueous solution or 200 mL of 100% ethanol was added and heated. It was confirmed that extraction and filtration were difficult due to the gelation of the active ingredients at the beginning of the millennia in the ethanol extract or distilled water of 40% or less of the ethanol extracts prepared by each of the above production methods (FIG. 1). Therefore, in the subsequent experiments, an extract of ethanol aqueous solution or ethanol extracted by 60% or more was used.

The extracts of Chenille seeds were prepared at the temperature of 15 ° C and then used for the experiment without further drying.

The propolis extract was prepared by drying bee propolis and then pulverizing it to 200 mesh or less to prepare a glue powder. Then, 2,000 g of 70% ethanol aqueous solution was added to 200 g of the glue powder, The mixture was continuously stirred for 24 hours to prepare an extract. The above-mentioned brine extract solution was fermented at 30 DEG C for 30 days and then filtered to prepare a pungent fermented broth. The pungent fermented broth was heated at 70 DEG C for 14 days with stirring to prepare a propolis extract. When fermenting the fermentation broth extract, the fermentation takes place slowly at a temperature lower than 20 ° C., and the fermentation progresses too rapidly at 40 ° C., and it is preferable that the fermentation proceeds at a temperature condition of 20 ° C. to 40 ° C. at 30 ° C. .

< Example . Antibacterial and moisturizing effects>

The antimicrobial activity of the propolis extract prepared in the above Preparation Example was compared with that of Pungyo Extract and Propolis Extract, which were extracted from 70% ethanol aqueous solution and the original raw material, in order to confirm the degree of improvement of antibacterial activity during the extraction process.

The paper disc method was used to evaluate the antibacterial activity. Specifically, Gram positive bacteria Staphylococcus aureus KCTC 1928, Gram negative bacteria Salmonella typhimirium KCTC 1925 and Escherichia coli KCTC 1923 were purchased from KCTC (Korean Collection for Type Cultures). Each strain was cultured in LB medium (1% bactotryptone, 0.5% bactoast extract, 1% (mid-log phase), diluted to 1 × 10 ⁴ cells / 100 μl with 1% bactopeptone medium, plated on a solid medium, and then paper discs were prepared. / RTI &gt; Thereafter, after culturing at 37 ° C for 24 hours, the diameter of the culture medium was measured, and the antibacterial effect was measured three times. Of the strains, Staphylococcus aureus ) are shown in Table 1 below.

sample Inhibition Zone (40ul assay, cm) Pong 1.25 Bonggyo Extract 1.2 Propolis extract 1.66

As shown in Table 1, it was confirmed that the extract of propolis obtained by extraction and fermentation was significantly improved in antimicrobial activity compared with the extract of Pungyo or Pungyo.

Soap was prepared using the propolis extract having improved antimicrobial effect.

Specifically, 200 g of soap chip (MP soap base) was weighed and placed in a pan and melted using a hot plate. When the soap base was completely dissolved, the propolis extract and the perennial extract were added to each amount, and the mixture was stirred well. Then, a propolis extract and a well-mixed soap base were poured into the soap mold. Thereafter, the mixture was aged at room temperature for 3 hours and hardened. Then, the completely hardened soap was taken out from the soap frame to prepare a soap. The soap prepared above is shown in Fig. 2

As shown in FIG. 2, a soap (upper portion) to which an excessively high amount of propolis extract was added, specifically, a propolis extract 10% (w / w, upper left portion) and a propolis extract 5% (w / w, left upper part) or propolis extract was 1% (w / w, lower left) compared with 0.1% (w / w, right lower part) of propolis extract.

3 shows the result of measuring the antibacterial activity against Staphylococcus aureus using the above-mentioned four kinds of soaps.

As shown in FIG. 3, when the propolis extract solution of 1% (w / w) or more was added, a definite inhibitory effect was confirmed, and it was confirmed that as the propolis extract content was increased, the size of the inhibitory ring was increased and the antibacterial activity was increased .

Regarding the moisturizing effect of the soap containing the propolis extract and the tsunami extract, 0.5% (w / w) based on the total weight of the extract of the tsunami extract using 50% ethanol aqueous solution and 100% ethanol was prepared.

A preliminary test on the moisturizing efficacy of the soap prepared was performed.

Specifically, in the preliminary moisturizing test, changes in moisture content were measured before and 30 minutes after washing. The moisture content before and after cleaning was compared with 100 before cleaning, and the results are shown in FIG.

As shown in FIG. 4, it was confirmed that the soap added with the extract of Chenille seeds showed better skin moisture than the soap added with propolis extract alone. In comparison with the case of adding 50% ethanol aqueous solution extract, It was confirmed that the addition of the extract had a better moisturizing effect.

Claims (7)

Propolis Extract and Opuntia humifusa extract as an active ingredient. The method according to claim 1,
The propolis extract is prepared by drying a cap bristle followed by pulverization to 200 mesh or less to prepare a cap bristle powder; 500 to 2,000 parts by weight of a 50% to 90% ethanol aqueous solution based on 100 parts by weight of the glue powder is added to the glue powder, and the mixture is continuously stirred and stirred at 10 ° C to 20 ° C for 10 hours to 30 hours to prepare an extract. Manufacturing steps; A fermentation broth fermentation broth for fermenting the fermented broth extract at 20 ° C to 40 ° C for 20 days to 45 days, followed by filtration to produce a fermented broth fermentation broth; And a method for producing propolis extract comprising the step of preparing a propolis extract by stirring the fermented broccoli fermentation broth at 60 ° C to 80 ° C for 10 to 20 days with stirring at every hour, Cosmetic composition. The method according to claim 1,
The cosmetic composition according to any one of claims 1 to 5, wherein the citrus extract is solvent-extracted with an ethanol aqueous solution of 60% or more or ethanol. The method of claim 3,
The cosmetic composition according to any one of claims 1 to 3, wherein the quinine-leaf extract is solvent-extracted with ethanol. The method according to claim 1,
Wherein the content of the propolis extract is 1% (w / w) based on the total weight of the cosmetic composition, and the content of the extract is 1% (w / w) based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the cosmetic composition is an antibacterial soap composition having a moisturizing activity. The method according to claim 1,
Wherein the cosmetic composition further comprises an albumen extract.

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