KR102452738B1 - A handwash composition containing lactic acid bacteria culture product of cactus flower - Google Patents

Abstract

The present invention relates to a hand wash composition containing a lactic acid bacteria culture of cactus flower, and a method for manufacturing the same. More specifically, the present invention relates to: a hand wash composition which adds the lactic acid bacteria culture of cactus flower to improve antibacterial and detergency, and has reduced skin irritation of the hand wash composition through mixed filtered water; and a method for manufacturing the same.

Description

Hand wash composition containing cactus flower lactic acid bacteria culture and manufacturing method thereof {A HANDWASH COMPOSITION CONTAINING LACTIC ACID BACTERIA CULTURE PRODUCT OF CACTUS FLOWER}

The present invention relates to a hand wash composition comprising a cactus flower lactic acid bacteria culture and a manufacturing method thereof, in particular, by adding a cactus flower lactic acid bacteria culture to improve antibacterial properties and cleaning power, and skin irritation of the hand wash composition through mixed filtered water It relates to a hand wash composition comprising a cactus flower lactic acid bacteria culture with reduced , and a method for producing the same.

Recently, as people's interest in health and hygiene is increasing due to the corona virus, the consumption of hand wash to wash fine dust and contaminants from hands is gradually increasing.

The human hand is home to an average of 10,000 to 10 million bacteria, and since it is the part of the body that comes into contact with the outside the most, it is easy to get infected with bacteria. You can prevent various diseases just by washing your hands with soap or hand wash.

In general, hand wash consists of a synthetic surfactant as an active ingredient and washes away dirt with foaming surfactant and water, and isopropanol, isopropyl alcohol, etc. It is classified as an antiseptic type that kills and antibacterializes. At this time, the ingredients of hand wash are generally harmful to the human body because synthetic surfactants that can irritate the skin are added.

Synthetic surfactants are sodium lauryl sulfate (Sodium Lauryl Sulfate), sodium laureth sulfate (Sodium Laureth Sulfate), Ammonium Lauryl Sulfate (Ammonium Lauryl Sulfate), preservatives, as it has excellent cleaning power, shampoo, laundry detergent, dish detergent, It is added to soap and body wash, but if it remains on the skin, it easily penetrates into the human body and destroys the skin wall. This can be induced

Therefore, hand washes containing synthetic surfactants, animal-derived ingredients, preservatives, and artificial colors are difficult to use for sensitive skin, infants, or patients with skin diseases. There is a need for various studies on hand wash, such as developing wash.

In this regard, Korea Patent Publication No. 10-2226844 is useful for diagnosing and improving cellular diseases, including microgold particles, and at the same time exhibiting excellent target directivity and improvement effect on specific cells, thereby reducing toxicity to the human body and destroying sebaceous glands. Disclosed is a cosmetic composition that has excellent efficacy, such as antibacterial action, and is useful for improving acne without side effects on the skin and that can be used in lotions, skins, serums, essences, mask packs, creams, powders, hand washes, etc. have.

However, as consumers' interest in hand wash increases, the needs for hand wash such as antibacterial power, cleansing power, moisturizing power, and additional skin beauty effect are diversified, and the need for research on hand wash is increasing.

Korean Patent Publication No. 10-2226844

In order to solve the above problems, the present invention improves antibacterial and cleaning power by adding a cactus flower lactic acid bacteria culture, and a hand containing a cactus flower lactic acid bacteria culture that reduces skin irritation of a hand wash composition through mixed filtered water A wash composition and a method for preparing the same are provided.

The purpose is a lactic acid bacteria culture step of drying and pulverizing cactus flowers, then applying the powder to a medium, inoculating lactic acid bacteria and culturing for 1 to 3 days;

a culture extraction step of separating the culture from the cultured lactic acid bacteria medium;

50% by weight of activated carbon, 20% by weight of tourmaline powder, 15% by weight of pomegranate peel pulverized product, and 15% by weight of cypress wood chips 70% by weight of filtered water prepared by filtering distilled water 3-5 times and hydrogen in water preparing mixed filtered water by mixing 30% by weight of hydrogen water prepared by injecting;

preparing a cleaning mixture by adding eucalyptus oil, a surfactant, a cleaning aid, a sequestering agent, and the mixed filtered water to the cactus flower lactic acid bacteria culture; It can be achieved by a method for producing a hand wash composition comprising a cactus flower lactic acid bacteria culture, including.

Specifically, the washing mixture preparation step may further include adding 2-4 parts by weight of pulverized plum seeds and 0.5-1 parts by weight of melon seed extract with respect to 100 parts by weight of the washing mixture.

Specifically, the step of extracting the culture may further include fractionating the culture obtained after extracting the culture with n-hexane, and then removing the solvent of the fraction and pulverizing it.

Specifically, the lactic acid bacteria may be one or more lactic acid bacteria consisting of Lactobacillus reuteri, Lactobacillus salivarius, and Lactobasillus plantarum.

Specifically, the cactus may be a royal cactus (opuntia monacantha Haw.).

The hand wash composition containing the cactus flower lactic acid bacteria culture according to the present invention has improved antibacterial properties and cleaning power by adding the cactus flower lactic acid bacteria culture, and it is used as a hand wash composition because it reduces skin irritation of the hand wash composition through the mixed filtered water. This is possible.

1 is a flowchart for explaining a method of manufacturing a hand wash composition comprising a cactus flower lactic acid bacteria culture according to an embodiment.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. However, since various changes may be made to the embodiments, the scope of the patent application is not limited or limited by these embodiments. It should be understood that all modifications, equivalents and substitutes for the embodiments are included in the scope of the rights.

Specific structural or functional descriptions of the embodiments are disclosed for purposes of illustration only, and may be changed and implemented in various forms. Accordingly, the embodiments are not limited to a specific disclosure form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical spirit.

Although terms such as first or second may be used to describe various elements, these terms should be interpreted only for the purpose of distinguishing one element from another. For example, a first component may be termed a second component, and similarly, a second component may also be termed a first component.

When a component is referred to as being “connected” to another component, it may be directly connected or connected to the other component, but it should be understood that another component may exist in between.

Terms used in the examples are used for the purpose of description only, and should not be construed as limiting. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present specification, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It is to be understood that this does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in a variety of different forms, only these embodiments allow the disclosure of the present invention to be complete, and common knowledge in the technical field to which the present invention belongs It is provided to fully inform the possessor of the scope of the invention, and the present invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs. have meaning Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in an embodiment of the present invention, an ideal or excessively formal meaning is not interpreted as

The shapes, sizes, proportions, angles, numbers, etc. disclosed in the drawings for explaining the embodiments of the present invention are illustrative and the present invention is not limited to the illustrated matters. In addition, in describing the present invention, if it is determined that a detailed description of a related known technology may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted. When 'including', 'having', 'consisting', etc. mentioned in this specification are used, other parts may be added unless 'only' is used. When a component is expressed in the singular, the case in which the plural is included is included unless specifically stated otherwise.

In interpreting the components, it is interpreted as including an error range even if there is no separate explicit description.

The size and thickness of each component shown in the drawings are illustrated for convenience of description, and the present invention is not necessarily limited to the size and thickness of the illustrated component.

Each feature of the various embodiments of the present invention may be partially or wholly combined or combined with each other, and as those skilled in the art will fully understand, technically various interlocking and driving are possible, and each embodiment may be independently implemented with respect to each other. It may be possible to implement together in a related relationship.

One aspect of the present application is a lactobacillus culture step of drying and pulverizing cactus flowers, then applying the powder to a medium and inoculating lactic acid bacteria for 1 to 3 days; a culture extraction step of separating the culture from the cultured lactic acid bacteria medium; 50% by weight of activated carbon, 20% by weight of tourmaline powder, 15% by weight of pomegranate peel pulverized product, and 15% by weight of cypress wood chips 70% by weight of filtered water prepared by filtering distilled water 3-5 times and hydrogen in water Mixing 30% by weight of hydrogen water prepared by injecting to prepare mixed filtered water; preparing a cleaning mixture by adding eucalyptus oil, a surfactant, a cleaning aid, a sequestering agent, and the mixed filtered water to the cactus flower lactic acid bacteria culture; It provides a method for producing a hand wash composition comprising a cactus flower lactic acid bacteria culture comprising a.

Hereinafter, a method of manufacturing a hand wash composition including a cactus flower lactic acid bacteria culture according to an embodiment of the present invention can be described with reference to FIG. 1 .

First, the cactus flowers are dried and pulverized, then the powder is applied to the medium, inoculated with lactic acid bacteria, and cultured for 1 to 3 days.

The cactus flower refers to a flower of a cactus, a plant belonging to the cactus family, a dicotyledonous plant, and in the cosmetic composition field, a cactus flower extract is sometimes mixed with an extract of baeknyeoncho, a fruit of a cactus, but the cactus flower in the present invention is It is used to distinguish it from baeknyeoncho. The extract of cactus flower has a moisturizing effect on the skin and is mainly used in cosmetic compositions.

In one embodiment, the cactus may be a king cactus (opuntia monacantha Haw.).

As the medium, various mediums capable of culturing lactic acid bacteria may be used, and specifically may be a liquid medium.

In one embodiment, the lactic acid bacteria may be one or more lactic acid bacteria consisting of Lactobacillus reuteri, Lactobacillus salivarius, and Lactobasillus plantarum.

Next, the culture is separated from the cultured lactic acid bacteria medium.

The culture separation may use a variety of separation methods used by those skilled in the art to separate cultures, specifically, separation through centrifugation or filtration may be used.

In one embodiment, the culture separation process may further include the step of fractionating the culture obtained after separating the culture with n-hexane, and then removing the solvent of the fraction and pulverizing it.

Next, 70% by weight of filtered water prepared by filtering distilled water 3-5 times through a filtrate consisting of 50% by weight of activated carbon, 20% by weight of tourmaline powder, 15% by weight of pomegranate peel pulverized product and 15% by weight of cypress wood chips, and 30% by weight of hydrogen water prepared by injecting hydrogen into water is mixed.

The activated carbon refers to a carbonaceous material having high adsorption properties. It is made by activating charcoal, etc., and is used for decolorization, purification, catalyst, gas mask, etc.

The tourmaline powder refers to powdered tourmaline, a crystalline mineral of a hexagonal system, also called tourmaline, and the chemical composition is a crystalline borosilicate mineral in which elements such as aluminum, iron, magnesium, sodium, lithium or potassium are mixed.

The pomegranate peel pulverized product refers to a pulverized product obtained by pulverizing the inner seed and pulp of a pomegranate fruit of a pomegranate which is a deciduous small tree in the family Pomegranate family, a dicotyledonous plant.

It means the pulverized cypress, an evergreen arboreous tree of the gymnosperm family Coniferaceae, into small chips. Cypress trees contain a lot of natural antibacterial substances called phytoncides, which have excellent sterilization effect.

Finally, eucalyptus oil, a surfactant, a cleaning aid, a sequestering agent and the mixed filtered water are added to the cactus flower lactic acid bacteria culture, and then mixed to prepare a cleaning mixture.

The eucalyptus oil refers to the oil collected from the leaves of eucalyptus trees, which are evergreen trees or shrubs of the dicotyledonous plant, gilded sheepwood, and is known to have excellent antioxidant, antibacterial, and anti-inflammatory activities, such as disinfectants, insect repellents, cosmetics, ointments, toothpastes, etc. being used in the field.

The surfactant refers to a substance that reduces the surface tension by adsorbing to the interface in a dilute solution, and serves to remove oily components and foreign substances from the skin, hair or teeth to be cleaned by being included in the detergent.

The cleaning aid means a component other than a surfactant added to the cleaning agent to enhance the cleaning action or to finish after cleaning. Specifically, the cleaning aid may be one or more cleaning aids added from the group consisting of sodium lactate, sodium tripolyphosphate, sodium silicate, sodium carbonate, CMC, optical brightener, bleach and enzyme.

The sequestering agent refers to a component that binds to and inactivates metallic ions that adversely affect the stability or properties of the detergent, and a representative example is ethylenediaminetetraacetic acid (EDTA).

In one embodiment, the mixing process may be to further include the step of adding 2 to 4 parts by weight of pulverized plum seeds and 0.5 to 1 parts by weight of melon seed extract with respect to 100 parts by weight of the washing mixture.

The pulverized plum seed refers to a pulverized product obtained by pulverizing the seeds remaining after removing the pulp of a plum, a fruit of a deciduous small arboreal plum tree in the Rosaceae family. The pulverized plum seeds in the present invention may be mixed in the hand wash composition and used as scrub particles.

The melon seed extract refers to an extract extracted from the seeds present in the fruit of a melon, an annual vine of the dicotyledonous plywood group Gourd, and is known to have an effect of improving constipation and lowering blood sugar.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

[Example 1]

First, the flowers of the king cactus (opuntia monacantha Haw.) were dried with hot air for 1 to 5 hours, then pulverized and powdered with a grinder, then the powder was applied to the MRS liquid medium, and Lactobacillus reuteri was inoculated and cultured for 2 days. .

After the cultured lactic acid bacteria medium was centrifuged at 1500 rpm for 15 minutes, only the supernatant was separated to separate the culture.

Next, 70% by weight of filtered water prepared by filtering distilled water three times through a filtrate composed of 50% by weight of activated carbon, 20% by weight of tourmaline powder, 15% by weight of pomegranate peel pulverized product and 15% by weight of cypress wood chips, and water Mixed filtered water was prepared by mixing 30% by weight of hydrogen water prepared by injecting hydrogen.

Eucalyptus oil, surfactant, cleaning aid, metal ion sequestrant and the mixed filtered water were added to the cactus flower lactic acid bacteria culture, and then mixed to prepare a washing mixture, thereby preparing a hand wash composition containing a cactus flower lactic acid bacteria culture. The component contents of the washing mixture are shown in Table 1 below. The prepared hand wash composition was named Example 1.

ingredient content (wt%) Cactus flower lactic acid bacteria culture 5 eucalyptus oil 2 Lauric Acid 3 propylene glycol 5.5 Decylglucoside 8 glycerin 4 EDTA-4Na 0.2 ethanol 15 mixed filtered water 57.3

[Example 2]

Prepared in the same manner as in Example 1, except that in the final mixing process of preparing the hand wash composition, 3 parts by weight of pulverized plum seeds and 1 part by weight of melon seed extract are added to 100 parts by weight of the washing mixture. The prepared hand wash composition was named Example 2.

[Example 3]

A hand wash composition prepared in the same manner as in Example 1, except that the culture separated in the culture separation process was fractionated with n-hexane, and then the solvent of the fraction was removed and powdered. 3 was named. The component contents of the washing mixture to which the weight changed by pulverization was applied are shown in Table 2 below.

ingredient content (wt%) Cactus Flower Lactobacillus Culture Powder 2 eucalyptus oil 2 Lauric Acid 3 propylene glycol 5.5 Decylglucoside 8 glycerin 4 EDTA-4Na 0.2 ethanol 15 mixed filtered water 60.3

[Comparative Example 1]

A hand wash composition prepared in the same manner as in Example 1, but without adding a cactus flower lactic acid bacteria culture to the washing mixture, was named Comparative Example 1.

[Comparative Example 2]

A hand wash composition prepared in the same manner as in Example 1, except that the mixed filtered water was replaced with distilled water, was named Comparative Example 2.

[Experimental Example 1: Comparison of cleaning power of hand wash compositions]

In order to check the cleaning power of the hand wash compositions prepared in Examples and Comparative Examples, lipstick was applied to the skin of the subject's forearm 5 times in a size of 2x1 cm in width and length, and pictures were taken before use. After 1 g of the hand wash compositions of Examples 1 to 3 and Comparative Examples 1 and 2 were applied to a position corresponding to each sample number, rubbed 20 times, and then washed by spraying water 30 times with a mouthwash bottle, and then a photograph was taken. The degree of cleaning power on the lipstick was confirmed by comparing the photos before and after cleaning using a colorimeter, and the results are shown in Table 3 below.

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Detergency (%) 80 86 83 57 68 Detergency (%): [(A-B)/A] × 100 (A: Redness of lipstick before washing, B: Redness of lipstick after washing)

As shown in Table 3, the cleaning power of Examples 1 to 3 was 80% or more, and it was confirmed that the cleaning power was very excellent compared to Comparative Examples 1 and 2. In particular, in the case of Comparative Example 1, it was confirmed that the cleaning power was significantly reduced compared to the Example, and it was confirmed that the cactus flower lactic acid bacteria culture affects the cleaning power.

[Experimental Example 2: Comparison of sterilization power of hand wash compositions]

In order to confirm the sterilization power of the hand wash compositions prepared in Examples and Comparative Examples, sterilization power evaluation was performed according to ASTM E 2315: 2016, and the results are shown in Table 4 below.

Test bacteria / sterilization power (%) initial concentration
(CFU/mL) Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Escherichia coli ATCC 25922 4.2X10 ⁶ 99.9 99.9 99.9 97.3 97.6 Pneumonia (Klebsiellapneumoniae ATCC 4352) 4.0X10 ⁶ 99.9 99.9 99.9 96.9 98.3 Pseudomonas aeruginosa ATCC 15442 5.9X10 ⁶ 99.9 99.9 99.9 97.2 98.0 Staphylococcus aureus ATCC 6538 6.1X10 ⁶ 99.9 99.9 99.9 96.5 97.7 Vibrio arahaemolyticus ATCC 7802 3.6X10 ⁶ 99.9 99.9 99.9 96.1 98.8 Sterilization power (%): [(A-B)/A] × 100 (A: Initial number of bacteria, B: Number of bacteria on test piece after contact)

As shown in Table 4, the detergent compositions of Examples 1 to 3 all showed a very high sterilization power of 99.9%, and Comparative Examples 1 and 2 also showed a sterilization power of 96% or more, confirming the excellent sterilization power of the hand wash composition according to the present invention. could

[Experimental Example 3: Comparison of moisturizing power of hand wash compositions]

In order to check the moisturizing power of the hand wash compositions prepared in Examples and Comparative Examples, all of the hand wash compositions of Examples 1 to 3 and Comparative Examples 1 and 2 were used over 30 days for 30 men and women with dry skin. After the test subjects were asked to fill out a questionnaire, subjective efficacy evaluation was performed on the improvement effect of dry skin. The survey was evaluated according to the criteria in Table 5, and the results are shown in Table 6 below.

Moisture power evaluation criteria evaluation score very good 5 points excellent 4 points usually 3 points insignificant 2 points Lack of moisture or dryness 1 point

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 moisturizing power 3.9 4.0 4.2 3.2 3.5

As shown in Table 6, it can be confirmed that the overall moisturizing power is excellent, and in particular, the detergent compositions of Examples 1 to 3 show superior moisturizing power than the compositions of Comparative Examples 1 and 2, so that the cactus flower lactic acid bacteria culture is a hand wash composition It was confirmed that the effect of increasing the moisturizing power of

[Experimental Example 4: Comparison of skin irritation of hand wash composition]

In order to check the skin irritation of the hand wash compositions prepared in Examples and Comparative Examples, the hand wash compositions of Examples 1 to 3 and Comparative Examples 1 and 2 were immersed in a cleaning solution diluted 1:1 with distilled water, respectively. . A part of the tissue was fixed and attached to the skin of 20 women and men in their 20s and 30s selected as sensory test personnel. After 24 hours, the tissue was removed and the degree of irritation was measured on the ICDRG (International Contact Dermatitis Research Group) scale after 90 minutes. was judged accordingly. The determination results were evaluated according to the determination criteria of Table 7 below, and are shown in Table 8 below.

evaluation evaluation score no irritation at all 0 points very weak redness 1 point weak redness 2 points strong flare 3 points Very strong redness or swelling 4 points

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 skin irritation 0.25 0.40 0.20 0.65 0.50

As shown in Table 8, all of the hand wash compositions except for Comparative Example 1 to which the cactus flower lactic acid bacteria culture was not added showed less than 0.5 skin irritation, confirming very little skin irritation.

Claims (3)

A lactobacillus culture step of drying and pulverizing the flowers of Opuntia monacantha Haw., then applying the powder to a medium, inoculating lactic acid bacteria and culturing for 1 to 3 days;
A culture extraction step of separating the culture from the cultured lactic acid bacteria medium and then fractionating the culture with n-hexane, removing the solvent, and pulverizing the culture;
50% by weight of activated carbon, 20% by weight of tourmaline powder, 15% by weight of pomegranate peel pulverized product, and 15% by weight of cypress wood chips 70% by weight of filtered water prepared by filtering distilled water 3-5 times and hydrogen in water Mixing 30% by weight of hydrogen water prepared by injecting to prepare mixed filtered water;
After adding eucalyptus oil, surfactant, cleaning aid, metal ion sequestrant and the mixed filtered water to the lactobacillus culture of King cactus flower, and mixing to prepare a cleaning mixture, 100 parts by weight of the cleaning mixture, Plum seed pulverized product 2 adding ~4 parts by weight and 0.5 to 1 parts by weight of melon seed extract; A method for producing a hand wash composition comprising a cactus flower lactic acid bacteria culture, comprising a.
delete delete

Images