CN104490679B - A kind of composite bactericidal hand cleanser and preparation method thereof - Google Patents
A kind of composite bactericidal hand cleanser and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of preparation method of composite bactericidal hand cleanser, 0.2 0.6g extractive of olive growing leaves is added to the water under the conditions of 22 28 DEG C, it is stirring evenly and then adding into 20 30ml glycerine, deionized water constant volume is added to 70 80ml, it is heated to the 12h of isothermal vibration 8 after 45 50 DEG C, then temperature is reduced to 22 28 DEG C, add ethanol and 0.05 0.1g triclosans that 20 30ml mass concentrations are 98 99.7%, it is 68 that pH value is adjusted after stirring, obtain composite bactericidal hand cleanser, the extractive of olive growing leaves is the extractive of olive growing leaves containing the Cleupin that mass concentration is 30 40%.The invention further relates to a kind of composite bactericidal hand cleanser.The composite bactericidal hand cleanser that the preparation method of composite bactericidal hand cleanser of the invention is prepared has the advantages that nontoxic, bactericide bacteriostasis are strong, volatility is good, stability is good and skin care.
Description
Technical field
The present invention relates to a kind of hand cleanser, more particularly to a kind of composite bactericidal hand cleanser and preparation method thereof.
Background technology
Epidemiology finds that most of communicable disease can be used as route of transmission and mediate by hand with pestology research, this
The hand of outer medical personnel is the major reason for causing inside-hospital infection, therefore is the sense of control hospital using rational hand-wrist bones
The important method of dye and infectious disease transmission.
Using soap as hand-washing article more than the past people.Soap has the characteristics of ease for use is poor, cross pollution is serious, from
And it is easily caused secondary pollution.At present, soap is just progressively replaced by hand cleanser in public places, and hand cleanser has cleaning skin care
Effect, sterilization, sterilized effect can be played in wherein adding specific composition.Hand cleanser is relatively low to skin irritatin degree, right
Skin moisturizing moistening effect is good, can arbitrarily move and put, and push type pump head can effectively prevent cross-infection, also can autogamy soap lye
Box, with it is easy to use, naturally expend low, usage cycles length, good water saving ability the features such as.
Current hand cleanser can be divided into common decontamination hand sanitizer and special efficacy hand cleanser according to its function, and the former is daily use chemicals
Articles for use, only general clean effect.As people's living standard is improved, the raising of people's health consciousness level and serious infectious diseases
Such as SARS, bird flu is threatened, particularly in public place people such as hospital, hotel toilet, schools to anti-bacterial hand lotion
Demand is greatly increased, and occupies the major part of special efficacy hand cleanser.
Using chemosterilant composition the disinfection hand cleanser of hospital, hotel and toilet at present more, such as chlorine containing acetic acid oneself
It is fixed, 75% ethanol, H2O2Or BZK etc., it is big to skin irritation, and particularly hospital medical personnel need prolonged and repeated
Using the infringement of hand skin can be caused, such as there is chap, decortication and dermatitis, absorb and induce an illness even with skin.At present
Handwashing disinfection agent containing plant component is less, its main cause be plant component be generally by alcohol, Solvent Extract methods it is effective
Composition, it has the property for being not readily dissolved in water, easily forming precipitation, can produce influence to the product characteristics of hand cleanser；It is non-in addition
Food plant active ingredient often has certain toxicity, easily induces the harm such as allergy.
In summary, in the prior art, most of simple Chinese herbal medicine extract dissolubility in water is poor, is made generally in and compares
Easily product characteristics are influenceed larger by precipitation, and simple plant extracts antibacterial ability is poor, typically can only achieve antibacterial effect
Really, it is more difficult to reach bactericidal effect in the short time.Artificial synthesized antiseptic sterilizing ability is strong, but ethanol and other disinfectants such as chlorine
Oneself determines, Iodophor, benzalkonium bromide too high levels, easily causes skin irritatin and toxicity.Because medicinal herb componentses are complicated, if will be a variety of
Plant extracts mixes, and anaphylactogen source can greatly increase, and easily induce allergic reaction, single plant extract adaptation population's phase
To extensive.
The content of the invention
The technical problems to be solved by the invention are：There is provided that a kind of properties of product are stable, nontoxic and sterilizing ability is strong answers
Mould assembly anti-bacterial hand lotion and preparation method thereof.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：
A kind of preparation method of composite bactericidal hand cleanser, by 0.2-0.6g extractive of olive growing leaves in 22-28 DEG C of bar
It is added to the water under part, is stirring evenly and then adding into 20-30ml glycerine, add deionized water constant volume to 70-80ml, is heated to 45-
Isothermal vibration 8-12h after 50 DEG C, is then reduced to 22-28 DEG C by temperature, and it is 98-99.7%'s to add 20-30ml mass concentrations
Ethanol and 0.05-0.1g triclosans, it is 6-8 that pH value is adjusted after stirring, and obtains composite bactericidal hand cleanser, described
The extractive of olive growing leaves for the Cleupin that it is 30-40% containing mass concentration that extractive of olive growing leaves, which is,.
The beneficial effects of the present invention are：
(1) by the mating reaction of glycerine and ethanol, the dissolubility of extractive of olive growing leaves is effectively improved, makes olive
The product stability for the composite bactericidal hand cleanser that leaf extract and triclosan (triclosan) are obtained after being sufficiently mixed and
Volatility is improved；
(2) by using extractive of olive growing leaves and mode associated with triclosan bactericide, simple plant extract had both been overcome
The not strong defect of thing bactericidal action, also overcoming exclusive use bactericide to produce skin stimulates and toxicity, and contains mass concentration and be
The extractive of olive growing leaves of the Cleupin of 35-40% can reach with triclosan combination and improve skin care and bactericidal action simultaneously
Excellent effect.
Embodiment
To describe technology contents, the objects and the effects of the present invention in detail, it is explained below in conjunction with embodiment.
The design of most critical of the present invention is：It is the olive of the Cleupin of 35-40% by using containing mass concentration
Leaf extract and mode associated with triclosan bactericide, so as to reach while improving the excellent effect of skin care and bactericidal action.
The present invention provides a kind of preparation method of composite bactericidal hand cleanser, by 0.2-0.6g extractive of olive growing leaves in
It is added to the water under the conditions of 22-28 DEG C, is stirring evenly and then adding into 20-30ml glycerine, adds deionized water constant volume to 70-80ml,
Isothermal vibration 8-12h after 45-50 DEG C is heated to, temperature is then reduced to 22-28 DEG C, it is 98- to add 20-30ml mass concentrations
99.7% ethanol and 0.05-0.1g triclosans, it is 6-8 that pH value is adjusted after stirring, and obtains composite bactericidal and washes
Hand liquid, the extractive of olive growing leaves is the extractive of olive growing leaves containing the Cleupin that mass concentration is 30-40%.
Principle of the invention is：Cleupin in the extractive of olive growing leaves of the present invention is wherein main antibacterial activity
Material, the safe, market price is relatively low, and is acted on anti-Gram-negative and positive bacteria, to Salmonella typhi, large intestine
Escherichia and Bacillus cercus inhibitory action are also obvious.Active ingredient in extractive of olive growing leaves even has suppression resistance to
The effect of the growth of medicine bacterium, including methicillin-resistant staphylococcus aureus (MRSA) and general tolerant Pseudomonas aeruginosa.Olive
Bitter glucoside has powerful anti-inflammatory, antioxidation.Decomposition of the ultraviolet to skin membrane lipid can be prevented, promotes fibrocyte generation glue
First albumen, reduces the secretion of fibrocyte glue unit enzyme, prevents the polysaccharide reaction of cell membrane, so that highly protective fibrocyte,
Ultraviolet can be resisted, the effects such as skin oxidative damage reaches anti-inflammatory skin care caused by free radical.Cleupin security is high, grinds
The person of studying carefully verifies its toxicity by continuously supplying 7 days Cleupins of Albino mice with the ultrahigh dose of 1000mg/kg, without discovery
Dead and toxicological effect.There is research that Cleupin is injected into the egg of fertilization, the no abnormality seen that grows of chicken embryo points out nothing
Obvious teratogenesis.Skin irritatin and allergy caused by the ethanol of conventional antimicrobial hand cleanser middle and high concentration are avoided in invention anti-
Should, relatively mild, the characteristics of appropriate ethanol has enhancing bactericidal effect and quickly volatilization is especially suitable for the work of the high rhythm of hospital
Working frequency requirement.The triclosan consumption that the present invention is added improves and washes in the lower limit of national legal usage amount (1-3%)
The bactericidal effect of natural bactericidal agent is strengthened while the security of hand liquid.Meanwhile, glycerol concentration is relatively low in the present invention, such as
Water is added without, simple ethanol+glycerine toxicity is larger, is not suitable for preparing safe hand cleanser, and lack water
In the case of easily crystallization separate out, it is simple that olive extract can not be completely dissolved using glycerine, form colloidal liquid wherein
Grain sense is substantially and toxicity is larger, simple bad using ethanol dissolubility, and precipitation is separated out after 3 days.And the glycerine of the present invention is dense
Degree and the cooperation of ethanol, can the protrusion of dissolved oil Fructus Canrii Albi extract well effect.
Knowable to foregoing description, the beneficial effects of the present invention are：
(1) by the mating reaction of glycerine and ethanol, the dissolubility of extractive of olive growing leaves is effectively improved, makes olive
The product stability of the composite bactericidal hand cleanser that leaf extract and triclosan (triclosan) are obtained after being sufficiently mixed and
Volatility is improved；
(2) by using extractive of olive growing leaves and mode associated with triclosan bactericide, both having overcome simple plant extract
The not strong defect of thing bactericidal action, also overcoming exclusive use conventional sterilants to produce skin stimulates and toxicity, and dense containing quality
Spending the extractive of olive growing leaves of the Cleupin for 35-40% can reach with triclosan combination while raising skin care and sterilization are made
Excellent effect.
Further, the preparation method of the extractive of olive growing leaves is：Olive growing leaves are crushed to 5-10 mesh, are then pressed
500g olive growing leaves add the ethanol that the ratio for the ethanol that 10-15L mass concentrations are 70-80% and mass concentration are 70-80%
Mixed, circumfluence distillation is carried out under the conditions of 65-75 DEG C, the mixture after extraction is carried out into filter cleaner, filtered crossing
Solution after slag is adsorbed by resin adsorption method, and the second that mass concentration is 50%-60% is added in the solution after absorption
Alcohol is eluted, and collects eluent, is then concentrated into no ethanol flavor, obtains concentrate, and the concentrate is added into ethyl acetate
Extracted, the concentrate is 1 with the volume ratio of ethyl acetate:(1/2-2/3), will be separated off the extraction of ethyl acetate layer
Liquid is evaporated and dried process, and the extractive of olive growing leaves is obtained.
Seen from the above description, the olive growing leaves that the preparation method of the extractive of olive growing leaves of the present embodiment is prepared are extracted
The main component of thing is Cleupin and hydroxytyrosol, and the wherein mass concentration of Cleupin is 30-40%.The oil of the present embodiment
The preparation method of Olive leaf P.E has the advantages that Cleupin refining effect is good.Olive growing leaves can carry out liquid before crushing
Chilled nitrogen, is beneficial to the destruction of the broken and cell membrane of olive leaf wall, so that beneficial to the carrying out of crushing operation.
Further, the olive growing leaves after crushing are immersed in the water, are heated to 50-60 DEG C, then adding mass concentration is
The cellulase of 0.2%-0.3%, regulation pH value is 3.5-4.5, stands 90-120min and is done under the conditions of 80-90 DEG C
It is dry.
Seen from the above description, cellulose is decomposed rapidly during the addition of cellulase can make olive growing leaves, is further carried
Follow-up Cleupin refining effect high.
Further, to be carried out under vacuum condition, the mode of the evaporation is rotary evaporation to the dried process.
Seen from the above description, drying has the advantages that rate of drying is fast and effect is good under vacuum condition, rotary evaporation side
The evaporation efficiency of formula is high.
Further, essence stock solution and/or glycerine are added before regulation pH value.
Seen from the above description, the composite bactericidal hand cleanser that prepared by the present invention, can be by adding before regulation pH value
Enter the compositions such as essence stock solution, reach the purpose of regulation fragrance, moisturizing effect is improved by adding glycerine.
Further, pH value is adjusted using citric acid.
The invention further relates to composite bactericidal hand cleanser prepared by a kind of preparation method of above-mentioned composite bactericidal hand cleanser.
Embodiment 1
The extractive of olive growing leaves 0.2g for 35-40% Cleupins containing mass concentration is taken, it is mixed with deionized water at room temperature
Close, magnetic agitation 1h, add 20ml glycerine, constant volume is heated under the conditions of 45 DEG C to 70ml, whirlpool concussion is stayed overnight in beaker
(8h), is down to room temperature, adds 99.7% ethanol 30ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH
Value 6-8, obtains the composite bactericidal hand cleanser of the present embodiment.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, volatilization
Hurry up, selected the study subject as skin irritation test and toxicological experiment, tested.Test result shows this reality
Apply the bacteriostasis rate of the composite bactericidal hand cleanser of example>90%, sterilizing rate>99.9%.
Embodiment 2
Only glycerine is heated to 50 DEG C, adds 0.05g triclosans, and ethanol mass concentration is 98% different, remaining
Condition is same as Example 1.
Embodiment 3
Only glycerine is heated to 48 DEG C, adds 0.07g triclosans, and ethanol mass concentration is 99% different, remaining
Condition is same as Example 1.
Embodiment 4：
The extractive of olive growing leaves 0.2g containing 35-40% Cleupins is taken, is mixed with deionized water at room temperature, magnetic agitation
1h, adds 25ml glycerine, constant volume to be heated under the conditions of 45 DEG C to 80ml, and whirlpool concussion overnight (8h), is down to room in beaker
Temperature, adds 99.7% ethanol 25ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains
The composite bactericidal hand cleanser of the present embodiment.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, and volatilization is fast, is selected
The study subject of skin irritation test and toxicological experiment is used as, is tested.Test result shows answering for the present embodiment
The bacteriostasis rate of mould assembly anti-bacterial hand lotion>90%, sterilizing rate>99.9%.
Embodiment 5
The extractive of olive growing leaves 0.2g of the Cleupin containing 35-40% is taken, is mixed at room temperature with deionized water, magnetic agitation
1h, adds 30ml glycerine, constant volume to be heated under the conditions of 45 DEG C to 75ml, and whirlpool concussion overnight (8h), is down to room in beaker
Temperature, adds 99.7% ethanol 20ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains
The composite bactericidal hand cleanser of the present embodiment.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, and volatilization is fast, is selected
The study subject of skin irritation test and toxicological experiment is used as, is tested.Test result shows answering for the present embodiment
The bacteriostasis rate of mould assembly anti-bacterial hand lotion>90%, sterilizing rate>99.9%.
Embodiment 6
The extractive of olive growing leaves 0.4g containing 35-40% Cleupins is taken, is mixed with 50ml deionized waters at room temperature, magnetic force
1h is stirred, 30ml glycerine is added and is heated under the conditions of 45 DEG C, (8h) is stayed overnight in whirlpool concussion in beaker, is down to room temperature, is added
99.7% ethanol 20ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains the present embodiment
Composite bactericidal hand cleanser.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, and volatilization is fast, and it is selected and is used as skin
The study subject of skin irritant experiment and toxicological experiment, is tested.Test result shows the composite bactericidal of the present embodiment
The bacteriostasis rate of hand cleanser>90%, sterilizing rate>99.9%.
Embodiment 7
The extractive of olive growing leaves 0.4g containing 35-40% Cleupins is taken, is mixed with 50ml deionized waters at room temperature, magnetic force
Stirring 1h, adds 20ml glycerine to be heated under the conditions of 45 DEG C, and whirlpool concussion overnight (8h), is down to room temperature in beaker, adds
99.7% ethanol 30ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains the present embodiment
Composite bactericidal hand cleanser., in clarification, transparence, volatilization is fast, is selected as skin for the composite bactericidal hand cleanser of acquisition
The study subject of skin irritant experiment and toxicological experiment, is tested.Test result shows the composite bactericidal of the present embodiment
The bacteriostasis rate of hand cleanser>90%, sterilizing rate>99.9%, the example solution is most clarified, transparent, and proterties is optimal, and volatilization is fast, is selected
It is used as skin irritation test and the study subject of toxicological experiment.
Embodiment 8
The extractive of olive growing leaves 0.4g containing 35-40% Cleupins is taken, is mixed with 50ml deionized waters at room temperature, magnetic force
1h is stirred, 25ml glycerine is added and is heated under the conditions of 45 DEG C, (8h) is stayed overnight in whirlpool concussion in beaker, is down to room temperature, is added
99.7% ethanol 25ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains the present embodiment
Composite bactericidal hand cleanser.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, and volatilization is fast, and it is selected and is used as skin
The study subject of skin irritant experiment and toxicological experiment, is tested.Test result shows the composite bactericidal of the present embodiment
The bacteriostasis rate of hand cleanser>90%, sterilizing rate>99.9%.
Embodiment 9
The extractive of olive growing leaves 0.6g of the Cleupin containing 35-40% is taken, is mixed at room temperature with 50ml deionized waters, magnetic force
1h is stirred, 20ml glycerine is added and is heated under the conditions of 45 DEG C, (8h) is stayed overnight in whirlpool concussion in beaker, is down to room temperature, is added
99.7% ethanol 30ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains the present embodiment
Composite bactericidal hand cleanser., in clarification, transparence, volatilization is fast, is selected as skin for the composite bactericidal hand cleanser of acquisition
The study subject of skin irritant experiment and toxicological experiment, is tested.Test result shows the composite bactericidal of the present embodiment
The bacteriostasis rate of hand cleanser>90%, sterilizing rate>99.9%.
Embodiment 10
The extractive of olive growing leaves 0.6g containing 35-40% Cleupins is taken, is mixed with 50ml deionized waters at room temperature, magnetic force
Stirring 1h, adds 25ml glycerine to be heated under the conditions of 45 DEG C, and whirlpool concussion overnight (8h), is down to room temperature in beaker, adds
99.7% ethanol 25ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains the present embodiment
Composite bactericidal hand cleanser.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, and volatilization is fast, and it is selected and is used as skin
The study subject of skin irritant experiment and toxicological experiment, is tested.Test result shows the composite bactericidal of the present embodiment
The bacteriostasis rate of hand cleanser>90%, sterilizing rate>99.9%.
Embodiment 11
The extractive of olive growing leaves 0.6g containing 35-40% Cleupins is taken, is mixed with 50ml deionized waters at room temperature, magnetic force
1h is stirred, 30ml glycerine is added and is heated under the conditions of 45 DEG C, (8h) is stayed overnight in whirlpool concussion in beaker, is down to room temperature, is added
99.7% ethanol 20ml, adds 0.1g triclosans, mixes 1h, and citric acid sets up pH value 6-8, obtains the present embodiment
Composite bactericidal hand cleanser.The composite bactericidal hand cleanser of acquisition is in clarification, transparence, and volatilization is fast, and it is selected and is used as skin
The study subject of skin irritant experiment and toxicological experiment, is tested.Test result shows the composite bactericidal of the present embodiment
The bacteriostasis rate of hand cleanser>90%, sterilizing rate>99.9%.
Effect analysis
1st, character observation
The composite bactericidal hand cleanser that embodiment 1-11 is obtained is clear, light brown liquid, is smooth to the touch moist, fragrant
Taste is pleasant, stands 120 at 4 DEG C and at room temperature respectively, without Precipitation, has no muddy.
2nd, fungistatic effect is tested
According to Ministry of Health of the People's Republic of China《Disposable Sanitary Accessory sanitary standard》(GB15979-1995) and
《Disinfection technology standard》The related request of (2008 editions) use staphylococcus aureus (ATCC 6538), Escherichia coli (8099),
Candida albicans (ATCC 10231) washes down test organisms 24h slant cultures with PBS as experimental subjects, is made bacteria suspension.
The composite bactericidal hand cleanser (5mL) of the invention obtained and control sample liquid 4 is taken to manage.Above-mentioned bacteria suspension is taken, respectively each compound
100uL is added dropwise in type anti-bacterial hand lotion and comparison liquid (PBS), uniform to mix, effect 2,5,10,20min, sample liquid respectively
In (0.5mL) input PBS containing 4.5mL test tube, fully mix, wherein 2-3 dilution factor is taken after dilution, is drawn respectively
0.5mL, is placed in two plates, with cool to 40-45 DEG C of nutrient agar (bacterium) or sabouraud's agar (yeast
Bacterium) 15mL pours into, 35 DEG C of ± 2 DEG C of culture 48h (bacterium) or 72h (saccharomycete), does viable bacteria colony counting.As a result this hair is shown
It is (actual super that bacteriostasis rate of the composite bactericidal hand cleanser of bright acquisition within 2,5,10,20min action time has exceeded 90%
Cross 99.9%), meet the antibacterial standard of disposable sanitary articles.
3rd, bactericidal effect
According to Ministry of Health of the People's Republic of China《Disposable Sanitary Accessory sanitary standard》(GB15979-1995) and
《Disinfection technology standard》The related request of (2008 editions) use staphylococcus aureus (ATCC 6538), Escherichia coli (8099),
Candida albicans (ATCC 10231) carries out nertralizer experiment, is successfully found in hand cleanser stoste first as experimental subjects
Be the PBS solution containing 0.5% lecithin and 5% Tween 80 with agent, after test organisms 24h slant cultures are washed down with PBS, make
Into bacteria suspension, above-mentioned bacteria suspension is taken, respectively in the composite bactericidal hand cleanser and comparison liquid for adding the present invention of 5ml to obtain
(PBS) 100uL, effect 2,5,10,20min are added dropwise in, it is uniform to mix, sampling liquid 0.5ml inputs corresponding nertralizer containing 4.5mL
In test tube, fully mix, make appropriate dilution, then take wherein 2-3 dilution factor, 0.5mL is drawn respectively, be placed in two plates,
Poured into cool to 40-45 DEG C of nutrient agar (bacterium) or sabouraud's agar (saccharomycete) 15mL, rotate flat
Ware, makes its full and uniform, and flat board, 35 DEG C of ± 2 DEG C of culture 48h (bacterium) or 72h (saccharomycete) are overturn after agar solidification, viable bacteria is done
Colony counting.As a result show that the composite bactericidal hand cleanser that the present invention is obtained exceedes in 2,5,10,20min sterilizing rates
99.9%, meet the sterilized standard of disposable sanitary articles.
4th, acute oral toxicity is tested
According to Ministry of Health of the People's Republic of China《Disposable Sanitary Accessory sanitary standard》(GB15979-1995) and
《Disinfection technology standard》The related request of (2008 editions), selects once test method(s), 18-20g Kun Ming mices 20 to greatest extent
Only, male and female half and half, the composite bactericidal hand cleanser that total amount is obtained for the present invention of 5g/kg is given in 24h points of 2 gavage approach,
Continuous Observation 15 days, has no dead, compares with control group and gives hand cleanser stoste body weight increase without substantially change, conscience kidney device
Coefficient does not find obvious pathological change without obvious change.Result of the test shows that the composite bactericidal hand cleanser that the present invention is obtained belongs to
In actual nontoxic rank.
5th, multiple intact skin stimulation test
According to Ministry of Health of the People's Republic of China《Disposable Sanitary Accessory sanitary standard》(GB15979-1995) and
《Disinfection technology standard》The related request of (2008 editions), selects continuous skin irritation test, 3 healthy new zealand white rabbits, body
Weight 2.2-2.5kg, the skin of 24h hair removeies removal backbone both sides, the composite bactericidal that the 0.5ml present invention is obtained is washed before experiment
Hand liquid is directly dripped in area on the side unhairing intact skin of 2.5cm × 2.5cm, opposite side skin of unhairing is using warm distilled water
0.5ml drops are applied.The application time is 4h.After off-test, residual tested material is removed with warm water or nonirritant solvent.Smear daily
Once, 14d is continuously smeared, is scored (fraction is higher, and excitant is stronger) according to erythema oedema situation.Result of the test shows, applies
There are 3 family's rabbit skins of composite bactericidal hand cleanser of present invention acquisition without obvious erythema, oedema, ulceration and inflammation, it is overall
Scoring is less than control group, points out have certain skin care effect and without skin irritation.
6th, stability test
The composite bactericidal hand cleanser that the present invention is obtained is placed in 54-57 DEG C of stability of drug products experimental box 14 days, is kept
Relative humidity ＞ 75%, tests its antibacterial and bactericidal property according to the method described above after 14 days, as a result show its it is antibacterial and
Bactericidal property is without change.
In summary, the composite bactericidal that the preparation method for the composite bactericidal hand cleanser that the present invention is provided is prepared is washed one's hands
Liquid had both overcome the not strong defect of simple plant extracts bactericidal action, also overcome exclusive use bactericide to skin produce stimulate and
Toxicity, can reach while improve the excellent effect of skin care and bactericidal action, with nontoxic, bactericide bacteriostasis are strong, volatility
Good, good and skin care the advantage of stability.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalents that bright description is made, or the technical field of correlation is directly or indirectly used in, similarly it is included in this hair
In bright scope of patent protection.
Claims (5)
1. a kind of preparation method of composite bactericidal hand cleanser, it is characterised in that by the extractive of olive growing leaves of 0.2-0.6g in
It is added to the water under the conditions of 22-28 DEG C, is stirring evenly and then adding into 20-30mL glycerine, adds deionized water constant volume to 70-80mL,
Isothermal vibration 8-12h after being heated to 45-50 DEG C, is then reduced to 22-28 DEG C by temperature, and it is 98- to add 20-30mL mass concentrations
99.7% ethanol and 0.05-0.1g triclosans, it is 6-8 that pH value is adjusted after stirring, and obtains composite bactericidal and washes
Hand liquid, the extractive of olive growing leaves for the Cleupin that it is 30-40% containing mass concentration that the extractive of olive growing leaves, which is,；
The preparation method of the extractive of olive growing leaves is：Olive growing leaves are crushed to 5-10 mesh, by the olive growing leaves after crushing
It is immersed in the water, is heated to 50-60 DEG C, be subsequently adding the cellulase that mass concentration is 0.2%-0.3%, regulation pH value is 3.5-
4.5, stand 90-120min and be dried under the conditions of 80-90 DEG C, then add 10-15L mass concentrations by 1g olive growing leaves
Ratio for 70-80% ethanol is mixed with the ethanol that mass concentration is 70-80%, and heat is carried out under the conditions of 65-75 DEG C
Refluxing extraction, filter cleaner is carried out by the mixture after extraction, and the solution after filter cleaner is inhaled by resin adsorption method
It is attached, the ethanol that mass concentration is 50%-60% is added in the solution after absorption and is eluted, eluent is collected, then concentrates
To without ethanol flavor, concentrate is obtained, concentrate addition ethyl acetate is extracted, the concentrate and ethyl acetate
Volume ratio is 1:(1/2-2/3), the extract that will be separated off ethyl acetate layer is evaporated and dried process, and the oil is obtained
Olive leaf P.E.
2. the preparation method of composite bactericidal hand cleanser according to claim 1, it is characterised in that the drying process is
Carried out under vacuum condition, the mode of the evaporation is rotary evaporation.
3. the preparation method of composite bactericidal hand cleanser according to claim 1, it is characterised in that before pH value is adjusted
Add essence stock solution and/or glycerine.
4. the preparation method of composite bactericidal hand cleanser according to claim 1, it is characterised in that using citric acid to pH
Value is adjusted.
5. prepared by a kind of preparation method of composite bactericidal hand cleanser according to claim 1-4 any one compound
Anti-bacterial hand lotion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410755781.5A CN104490679B (en) | 2014-12-10 | 2014-12-10 | A kind of composite bactericidal hand cleanser and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410755781.5A CN104490679B (en) | 2014-12-10 | 2014-12-10 | A kind of composite bactericidal hand cleanser and preparation method thereof |
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| CN104490679A CN104490679A (en) | 2015-04-08 |
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| AU2016410550B2 (en) * | 2016-06-20 | 2019-06-06 | Essity Hygiene And Health Aktiebolag | Hand sanitizing composition |
| CN108578346A (en) * | 2018-08-01 | 2018-09-28 | 杨林 | Skin-care antibacterial hand sanitizer and preparation method thereof |
| CN113520940A (en) * | 2021-07-07 | 2021-10-22 | 苏州麻朵纺织科技有限公司 | Natural plant-extracted disinfection type hand sanitizer with natural antibacterial and antiviral functions |
| CN113679623A (en) * | 2021-09-13 | 2021-11-23 | 上海西西艾尔启东日用化学品有限公司 | Antibacterial hand cream and cream emulsification system antibacterial performance test method |
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