CN104815096A - Nano composite skin disinfection emulsion and preparation method of same - Google Patents
Nano composite skin disinfection emulsion and preparation method of same Download PDFInfo
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Abstract
The invention discloses a nano composite skin disinfection emulsion and a preparation method of the same. The nano composite skin disinfection emulsion is composed of a double-chain quaternary ammonium salt compound, an inorganic nano antibacterial substance, an emulsifier, aloe extract liquid and water, wherein the double-chain quaternary ammonium salt compound is didecyldimethylammonium bromide, didecyldimethylammonium chloride or bis(dodecyldimethyl)ethylene diammonium bromide, and the inorganic nano antibacterial substance is nano zinc oxide, nano silver oxide or nano titanium oxide, and the aloe extract liquid is raw liquid from aloe. The emulsion, when acting on staphylococcus aureus, escherichia coli and candida albicans for 1 min, can reach 100% in killing ratio all. The emulsion is high in inhibiting rates of 6 h, 12 h, 24 h and 48 h on natural bacteria. When acting on hands of a subject for 1 min, the emulsion is higher than 90% in average killing rate on natural bacteria. The emulsion is excellent in stability, can be safely used on human skin and mucosa, and is free of damage and stimulation on environment and human body.
Description
Technical field
The present invention relates to a kind of disinfection emulsion and preparation method thereof, particularly relate to a kind of nanometer composite skin disinfection emulsion and preparation method thereof.
Background technology
Skin and mucosa are effective barriers that human body resists that profit tissue is invaded in microorganism, but have abundant microorganism on its surface.In daily life, by opponent and Mucocutaneous sterilization, the infection of infectious intestinal disease, skin pyesis and genitourinary system can be prevented; In health system, carrying out the important leverage that skin degerming is modern medical service, is also control one of most important, the most simple and easy to do measure of hospital infection.
Skin and mucous membrane disinfection agent huge number conventional at present, but have defect more or less, as quaternary ammonium salt narrow antimicrobial spectrum, sterilizing ability is weak; Ethanol is large to skin and mucous membrane irritation; The povidone iodine bactericidal effect persistent period is shorter; Chlorhexidine acetate class easily produces Resistant strain.Traditional disinfection agent is difficult to the needs meeting clinical departments medical personnel hand disinfection completely, thus causes cross infection.Therefore, need to find New raxa, novel form or new compound prescription, to reach desirable skin degerming effect.
Summary of the invention
The object of this invention is to provide a kind of nanometer composite skin disinfection emulsion and preparation method thereof, this skin disinfection emulsion is by double-chain quaternary ammonium salt compound and inorganic nano antiseptic material compatibility, and add emulsifying agent and Aloe extract, both organic antibacterial bactericidal effect had been played good, fireballing feature, the features such as formula acquisition inorganic antiseptic has a broad antifungal spectrum, lasting effect are good, good heat resistance, not easily generation drug resistance can be made again, effectively can kill the staphylococcus aureus of skin surface, escherichia coli and Candida albicans.
Nanometer composite skin disinfection emulsion provided by the invention, it is made up of double-chain quaternary ammonium salt compounds, inorganic nano antiseptic material, emulsifying agent, Aloe extraction solution and water;
Described double-chain quaternary ammonium salt compounds is two (dodecyl dimethyl) ethylene diammonium of two decyl dimethyl ammonium bromide, double-track suspended self-propelled conveyor or bromination;
Described inorganic nano antiseptic material is nano zine oxide, nano phase ag_2 o or nano titanium oxide;
Described Aloe extraction solution is aloe stoste.
In above-mentioned nanometer composite skin disinfection emulsion, described emulsifying agent is by three normal-butyl phosphate (tributyl phosphate), sorbitol anhydride monopalmitate (sorbitan monopalmitate, Sorbitan monopalmitate; Commodity are called span 40 or this dish 40, Span 40, CAS accession number: 26266-57-9) and polyoxyethylenesorbitan sorbitan monooleate (Polyoxyethylenesorbitan monooleate; Sorbitan monooleate ethoxylate; Commodity are called Tween 80, Tween 80, CAS accession number: 9005-65-6) composition, volume ratio is 1:(2 ~ 3): (1 ~ 3), specifically can be 1:2:2.
In above-mentioned nanometer composite skin disinfection emulsion:
The mass body volume concentrations of described double-chain quaternary ammonium salt compounds is 1 ~ 5g/L;
The mass body volume concentrations of described inorganic nano antiseptic material is 0.1 ~ 0.5g/L, and particle diameter is 50 ~ 100nm;
The volume fraction of described emulsifying agent is 10% ~ 20%;
The volume fraction of described Aloe extraction solution is 20% ~ 30%;
Surplus is water.
In above-mentioned nanometer composite skin disinfection emulsion, described Aloe extraction solution can be aloe stoste, and aloe stoste is the normal juice of Aloe, specifically buys by commercial sources and obtains, also prepare by following method: filter after Aloe is squeezed the juice, get filtrate and be aloe stoste.
In above-mentioned nanometer composite skin disinfection emulsion, described double-chain quaternary ammonium salt compounds has that toxicity is low, nonirritant, without bad smell, can in features such as disinfections under hard water conditions; But traditional double-chain quaternary ammonium salt compounds disinfectant belongs to low effect disinfectants, and the Germicidal Efficacy stronger for drag is poor, and is better than gram positive bacteria to gram-negative bacteria killing effect;
The mass body volume concentrations of described double-chain quaternary ammonium salt compounds can be 1 ~ 5g/L, specifically can be 1 ~ 2g/L, 2 ~ 5g/L, 1g/L, 2g/L or 5g/L, preferred 1g/L;
In above-mentioned nanometer composite skin disinfection emulsion, described inorganic nano antiseptic material is preferably nano zine oxide, and described nano zine oxide has good anti-microbial property and chemical compatibility.Its Antibacterial Mechanism is mainly: (1) ZnOw and granule reach nanoscale, thus has special skin effect and high oxidation activity that nano material possesses, (2) zinc oxide is a kind of semi-conducting material, wherein has a large amount of elemental oxygen to discharge, (3) network structure that unique four needle-likes of ZnOw are formed is that antibacterial ion provides high efficiency of transmission path, when electronegative antibacterial touches the arbitrary position of network, due to positive and negative charge captivation, a large amount of zinc ion is come so that the speed of electric current is mobile, penetration cell wall, destroy synthesis (the Zuowan Zhou of thalline internal protein, Shikai Liu, Lixia Gu.Studies on the strength andwear resistance of tetratod-shaped ZnO whisker-reinforced rubber composites. [J] AppliedPolymer Science, 2001, 80:1520-24),
The mass body volume concentrations of described inorganic nano antiseptic material can be 0.1 ~ 0.5g/L, specifically can be 0.1g/L or 0.5g/L; Particle diameter can be 50 ~ 100nm.
In above-mentioned nanometer composite skin disinfection emulsion, the volume fraction of described emulsifying agent can be 10% ~ 20%, specifically can be 10% or 20%.
In above-mentioned nanometer composite skin disinfection emulsion, described Aloe extraction solution has inhibitory action to multiple-microorganism, research is had to show it to the antibacterial effect of gram positive bacteria a little more than gram negative bacteria, Main Function principle may be have an impact to the Peptidoglycan of bacteria cell wall, destroys the cell wall of antibacterial and reaches the object of antibacterial;
The volume fraction of described Aloe extraction solution can be 20% ~ 30%, specifically can be 20% or 30%.
The present invention, by double-chain quaternary ammonium salt compound and Aloe extraction solution use in conjunction, had both enhanced its antibacterial ability, turn increased its antimicrobial spectrum, and imparted the protective effect of formula to skin; Simultaneously, inorganic nano antiseptic material is combined with them, compound prescription antibacterial effect can be strengthened, and give this nanometer composite skin disinfection emulsion new holding effect antibacterial effect, the synergistic function of three makes nanometer composite skin disinfection emulsion of the present invention have good sterilizing function, considers that the toxicity of disinfectant should control at zone of reasonableness, the highest 5g/L that is limited to of double-chain quaternary ammonium salt content that the present invention uses, zinc oxide controls at 0.5g/L, and Aloe extraction solution is 30%.
In above-mentioned nanometer composite skin disinfection emulsion, described nanometer composite skin disinfection emulsion specifically can be following 1)-7) in any one:
1) double-chain quaternary ammonium salt compounds mass body volume concentrations 1 ~ 5g/L, inorganic nano antiseptic material mass volumetric concentration 0.1 ~ 0.5g/L, emulsifying agent volume fraction 10% ~ 20%, Aloe extraction solution volume fraction 20% ~ 30%, solvent are water;
2) double-chain quaternary ammonium salt compounds mass body volume concentrations 1 ~ 2g/L, inorganic nano antiseptic material mass volumetric concentration 0.1 ~ 0.5g/L, emulsifying agent volume fraction 10% ~ 20%, Aloe extraction solution volume fraction 20% ~ 30%, solvent are water;
3) double-chain quaternary ammonium salt compounds mass body volume concentrations 2 ~ 5g/L, inorganic nano antiseptic material mass volumetric concentration 0.1 ~ 0.5g/L, emulsifying agent volume fraction 10% ~ 20%, Aloe extraction solution volume fraction 20% ~ 30%, solvent are water;
4) double-chain quaternary ammonium salt compounds mass body volume concentrations 1g/L, inorganic nano antiseptic material mass volumetric concentration 0.5g/L, emulsifying agent volume fraction 10%, Aloe extraction solution volume fraction 30%, solvent are water;
5) double-chain quaternary ammonium salt compounds mass body volume concentrations 2g/L, inorganic nano antiseptic material mass volumetric concentration 0.5g/L, emulsifying agent volume fraction 20%, Aloe extraction solution volume fraction 20%, solvent are water;
6) double-chain quaternary ammonium salt compounds mass body volume concentrations 5g/L, inorganic nano antiseptic material mass volumetric concentration 0.1g/L, emulsifying agent volume fraction 10%, Aloe extraction solution volume fraction 20%, solvent are water;
7) double-chain quaternary ammonium salt compounds mass body volume concentrations 1g/L, inorganic nano antiseptic material mass volumetric concentration 0.5g/L, emulsifying agent volume fraction 20%, Aloe extraction solution volume fraction 30%, solvent are water.
Nanometer composite skin disinfection emulsion of the present invention is by a kind of disinfectant of double-chain quaternary ammonium salt compound, nano zine oxide and Aloe extraction solution compound use, and forms a kind of oil-in-water emulsion structure, and in this disinfectant, the diameter of drop is 10 ~ 30nm.
Invention further provides a kind of preparation method of above-mentioned nanometer composite skin disinfection emulsion, comprise the steps:, by after each component mixing in above-mentioned nanometer composite skin disinfection emulsion, described nanometer composite skin disinfection emulsion can be obtained through high pressure homogenize.
In the preparation method of above-mentioned nanometer composite skin disinfection emulsion, the concentration following according to component each in final products mixes:
The mass body volume concentrations of described double-chain quaternary ammonium salt compounds is 1 ~ 5g/L; The mass body volume concentrations of described inorganic nano antiseptic material is 0.1 ~ 0.5g/L; The volume fraction of described emulsifying agent is 10% ~ 20%; The volume fraction of described Aloe extraction solution is 20% ~ 30%.
The concrete steps of above-mentioned preparation method are: take (measuring) a certain amount of double-chain quaternary ammonium salt compound, Aloe extraction solution, adds respectively and fill in the beaker of distilled water, put agitator and stir, to complete mixed dissolution.Measured amounts compound emulsifying agent adds in above-mentioned mixed solution, stirs.Take a certain amount of nano zine oxide in proportion, add in mixed solution, put after agitator stirs, form through high pressure homogenizer emulsifying.
In above-mentioned preparation method, described high pressure homogenize carries out under 25 ~ 35Mpa, condition at 20 ~ 30 DEG C, and pressure specifically can be 25 ~ 30Mpa, 30 ~ 35Mpa, 20Mpa, 30Mpa or 35Mpa, and temperature specifically can be 20 ± 2 DEG C.
Preparation method of the present invention by above-mentioned formula through high pressure homogenize emulsion process, compared with magnetic force mixing method and the cutting machine mixing method that exceeds the speed limit, not only have stirring, shearing, high-speed impact effect, also utilize the comprehensive effect such as liquid decompression, expansion, blast under condition of high voltage, the emulsion utilizing this feature to obtain not only is uniformly dispersed, not stratified, and obtain relatively fine particle, reach good emulsifying effectiveness, therefore, this formula, while reduction double-chain quaternary ammonium salt content, still has good bactericidal property.
Present invention provides above-mentioned nanometer composite skin disinfection emulsion in skin degerming or the application prepared in disinfectant liquid for skin.
In above-mentioned application, described disinfectant liquid for skin can kill staphylococcus aureus, escherichia coli and Candida albicans.
The present invention has following beneficial effect:
The formula of nanometer composite skin disinfection agent of the present invention is not containing alcohols, and to mucocutaneous nontoxic non-stimulated, safety in utilization is better.Emulsion liquid not only makes formula have good outward appearance, also add its stability.
Experiment proves:
(1) complex emulsions of the present invention is to staphylococcus aureus, escherichia coli, Candida albicans effect 1min, and killing rate all reaches 100%; The simple double-chain quaternary ammonium salt solution or the double-chain quaternary ammonium salt that are significantly better than same amount add Aloe extraction solution, inorganic nano antiseptic substance solution.
(2) simulated field experiment display, the suppression ratio of nanometer composite skin disinfection emulsion to 6h, 12h, 24h, 48h of natural bacteria is significantly higher than the simple double-chain quaternary ammonium salt aqueous solution with same amount and double-chain quaternary ammonium salt adds Aloe extraction solution complex.
(3) skin on-the-spot disinfection experimental result display, this emulsion, to experimenter's hand effect 1min, is greater than 90% to natural bacteria average kill ratio.
(4) high temperature accelerated test display, after this emulsion deposits 14d at 54 DEG C, active constituent content rate of descent is 3.05%, has good stability.
(5) toxicity test shows that this emulsion can be used safely on human body skin, mucosa, to environment and human body without harm and stimulation.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Aloe extraction solution in following embodiment prepares all by the following method:
Get fresh aloe, be after the potassium permanganate sterilization of 0.11% with mass fraction, rinse well with circulating water, to squeeze out absinthe-green juice with juice extractor, by 5 layers of filtered through gauze.
Embodiment 1, prepare nanometer composite skin disinfection emulsion
Nanometer composite skin disinfection emulsion is prepared according to following step:
0.1g double-track suspended self-propelled conveyor is added in the beaker filling 40 ~ 60mL distilled water, puts agitator and stir, to dissolving completely.Slowly added by 30mL Aloe extraction solution in double-track suspended self-propelled conveyor solution, limit edged stirs.Suction pipe is drawn 10mL compound emulsifying agent (containing three normal-butyl phosphate 2mL, span 40 4mL, Tween 80 4mL) and is added in above-mentioned mixed solution, stirs.Take 0.05g in proportion, particle diameter is the nano zine oxide of 50 ~ 100nm, add in mixed solution, adding distilled water supplies after the rearmounted agitator of 100mL stirs by solution, through high pressure homogenizer at room temperature (20 DEG C ± 2 DEG C), 25Mpa operating pressure conditions is emulsified forms the opaque emulsion of milky.
In the present embodiment, in the disinfection emulsion prepared by mentioned component and method, each component final concentration is: double-track suspended self-propelled conveyor 1g/L, Aloe extraction solution 30% (v/v), nano zine oxide 0.5g/L, emulsifying agent accounts for 10% of this disinfection emulsion cumulative volume.In this emulsion, the diameter of drop is 10 ~ 30nm, reaches Nano grade.
Embodiment 2, prepare nanometer composite skin disinfection emulsion
Nanometer composite skin disinfection emulsion is prepared according to following step:
Two for 0.2g decyl dimethyl ammonium bromide is added in the beaker filling 40 ~ 60mL distilled water, puts agitator and stir, to dissolving completely.Slowly added by 20mL Aloe extraction solution in two decyl dimethyl ammonium bromide solution, limit edged stirs.Suction pipe is drawn 20mL compound emulsifying agent (containing three normal-butyl phosphate 4mL, span 40 8mL, Tween 80 8mL) and is added in above-mentioned mixed solution, stirs.Take 0.05g in proportion, particle diameter is the nano zine oxide of 50 ~ 100nm, add in mixed solution, adding distilled water supplies after the rearmounted agitator of 100mL stirs by solution, through high pressure homogenizer at room temperature (20 DEG C ± 2 DEG C), 35Mpa operating pressure conditions is emulsified forms the opaque emulsion of milky.
In the present embodiment, in the disinfection emulsion prepared by mentioned component and method, each component final concentration is: two decyl dimethyl ammonium bromide 2g/L, Aloe extraction solution 20% (v/v), nano zine oxide 0.5g/L, emulsifying agent accounts for 20% of this disinfection emulsion cumulative volume.In this emulsion, the diameter of drop is 10 ~ 30nm, reaches Nano grade.
Embodiment 3, prepare nanometer composite skin disinfection emulsion
Two for 0.5g bromination (dodecyl dimethyl) ethylene diammonium is added in the beaker filling 40 ~ 60mL distilled water, puts agitator and stir, to dissolving completely.Slowly added by 20mL Aloe extraction solution in two (dodecyl dimethyl) ethylene two ammonium salt solution of bromination, limit edged stirs.Suction pipe is drawn 10mL compound emulsifying agent (containing three normal-butyl phosphate 2mL, span 40 4mL, Tween 80 4mL) and is added in above-mentioned mixed solution, stirs.Take 0.01g in proportion, particle diameter is the nano zine oxide of 50 ~ 100nm, add in mixed solution, adding distilled water supplies after the rearmounted agitator of 100mL stirs by solution, through high pressure homogenizer at room temperature (20 DEG C ± 2 DEG C), 30Mpa operating pressure conditions is emulsified forms the opaque emulsion of milky.
In the present embodiment, in the disinfection emulsion prepared by mentioned component and method, each component final concentration is: two (dodecyl dimethyl) ethylene diammonium 5g/L of bromination, Aloe extraction solution 20% (v/v), nano zine oxide 0.1g/L, emulsifying agent accounts for 10% of this disinfection emulsion cumulative volume.In this emulsion, the diameter of drop is 10 ~ 30nm, reaches Nano grade.
Embodiment 4, prepare nanometer composite skin disinfection emulsion
0.1g double-track suspended self-propelled conveyor is added in the beaker filling 40 ~ 50mL distilled water, puts agitator and stir, to dissolving completely.Slowly added by 30mL Aloe extraction solution in two decyl dimethyl ammonium bromide solution, limit edged stirs.Suction pipe is drawn 20ml compound emulsifying agent (containing three normal-butyl phosphate 4mL, span 40 8mL, Tween 80 8mL) and is added in above-mentioned mixed solution, stirs.Take 0.05g in proportion, particle diameter is the nano zine oxide of 50 ~ 100nm, add in mixed solution, adding distilled water supplies after the rearmounted agitator of 100mL stirs by solution, through high pressure homogenizer at room temperature (20 DEG C ± 2 DEG C), 30Mpa operating pressure conditions is emulsified forms the opaque emulsion of milky.
In the present embodiment, in the disinfection emulsion prepared by mentioned component and method, each component final concentration is: double-track suspended self-propelled conveyor 1g/L, Aloe extraction solution 30% (v/v), nano zine oxide 0.5g/L, emulsifying agent accounts for 20% of this disinfection emulsion cumulative volume.In this emulsion, the diameter of drop is 10 ~ 30nm, reaches Nano grade.
The performance test of embodiment 5, nanometer composite skin disinfection emulsion
1, physicochemical property
After tested, the disinfection emulsion that above-described embodiment 1-4 prepares gained all meets following physicochemical character:
Outward appearance: milky is opaque
PH value: 8.10
Particle diameter (nm): 10 ~ 30nm under Electronic Speculum
Stability: adopt high temperature accelerated test, this emulsion places 14 days bactericidal active ingredient rate of descent≤10% at 54 DEG C of calorstats, has good stability.
2, bactericidal effect
The bactericidal assay of nanometer composite skin disinfection emulsion is as follows (test is all carried out with reference to " disinfection technology standard "), and acquired results if no special instructions, is embodiment 4 and prepares gained nanometer composite skin disinfection emulsion result of the test.
Nertralizer in following test is the phosphate buffer containing 2g/L glycine, 3g/L lecithin, 2.5% Tween 80 and 10% calf serum, can completely in and skin disinfection emulsion stock solution to the killing action of staphylococcus aureus, escherichia coli and Candida albicans, and on the composition of culture medium without impact, meet the requirement that " disinfection technology standard " specifies.
(1) different formulations composition is to the carrier quantitative germicidal test results contrast of different microorganisms
Solution A formulation: double-track suspended self-propelled conveyor 1g/L, Aloe extraction solution 30%, nano zine oxide 0.5g/L, emulsifying agent 20%.
Solution B formula: double-track suspended self-propelled conveyor 1g/L.
Solution C formula: double-track suspended self-propelled conveyor 1g/L, Aloe extraction solution 30%.
Solution D formula: double-track suspended self-propelled conveyor 1g/L, Aloe extraction solution 30%, nano zine oxide 0.5g/L.
All carry out carrier to solution A, B, C and D and soak quantitative disinfecting test, experimental bacteria is respectively staphylococcus aureus (ATCC6538), escherichia coli (8099) or Candida albicans (ATCC 10231).By the consumption of a slice bacterium sheet 5.0mL, A, B, C, D tetra-kinds of formula solution stock solutions (positive control is phosphate buffer PBS) are added in sterilized petri dishes.Put preheating 5min in 19 ~ 21 DEG C of water-baths, then add bacterium sheet.Effect certain hour, moves into bacterium sheet and is equipped with in the test tube of 5.0mL nertralizer, neutralization 10min.Beat test tube 80 times, mix with bacterium on eluting bacterium sheet.Get eluent 5.0mL and do count plate, calculate killing rate.Comparison is to the killing effect of microorganism, and acquired results is as shown in table 1.
Table 1, different formulations performance comparison result
Note: test temperature is 19 ~ 21 DEG C.The average bacterium number of positive controls is: staphylococcus aureus is 1350000cfu/ sheet, and escherichia coli are 3400000cfu/ sheet, and Candida albicans is 3200000cfu/ sheet.
As can be seen from upper table result, the average kill ratio of the nano combined disinfection emulsion stock solution of the present invention to staphylococcus aureus, escherichia coli and Candida albicans all reaches 100%, and good stability, therefore, double-chain quaternary ammonium salt compound and Aloe extraction solution, nano zine oxide and emulsifying agent compatibility, all have obvious synergistic function to staphylococcus aureus, escherichia coli, Candida albicans, bactericidal effect is significantly improved.
(2) to the comparison of natural bacteria holding effect antibacterial ability
Silica gel tube for subsequent use for sterilizing is soaked in respectively containing double-track suspended self-propelled conveyor 1g/L, in the nano combined disinfection emulsion stock solution (solution A) that the aqueous solution (solution C) of Aloe extraction solution 30% and the present invention prepare, immersion is taken out after 3 minutes and is dried completely.Separately get after undressed silica gel tube sterilizing as positive control.Getting above-mentioned silica gel tube puts in same plate, puts 4 plates altogether, uncoveredly puts Microbiological Lab's windowsill, take out in 6h, 12h, 24h, 48h with aseptic nipper, put into the test tube containing 5mL nertralizer, shake and strike rear standing 10min, observed result after inoculated and cultured 48 ~ 72h.Result of the test is in table 2.
Table 2, different disposal group place different time to the growth inhibited situation of natural bacteria
As can be seen from table 2 result, the rejection ability of nanometer composite skin disinfection emulsion of the present invention to natural bacteria is strong, compared with adding aloe liquid formula with double-chain quaternary ammonium salt, the compound prescription adding inorganic nano antiseptic material and emulsifying agent is significantly increased to the holding effect antibacterial ability of natural bacteria at 6h-48h.
(3) hand site test results
After the mutual fully rubbing of experimenter's both hands, left hand is sampled, as matched group of not sterilizing with cotton examination semar technique.Then, stock solution 3mL is smeared evenly at the right hand.Act on 2 minutes, the right hand is smeared and adopts sterilization group sample.During sampling, 5 refer to close up, and are wiped by sterile cotton and soak in being contained in invisible spectro 5mL nertralizer, then after extracting on tube wall, follow double rub 2 times, often embrocate one time, rotated once by cotton swab at finger curved surface from finger tip to finger.Cotton is wiped head to cut into dress nertralizer in vitro.Neutralization 10 minutes, beats test tube and wipes upper bacterium with eluting cotton and mix.Get eluent and do count plate, calculate killing rate.Result of the test shows, nanometer disinfection emulsion effect 1 minute, is 95.09% to the average kill ratio of 30 person-times of natural bacterias on hand.
3, toxicological test:
Test by " disinfection technology standard " (2002 editions) specific requirement, acquired results if no special instructions, is the result of the test that embodiment 4 prepares gained nanometer composite skin disinfection emulsion.
(1) acute oral toxicity test
Body weight is selected to be that Kunming outbreeding system healthy mice 20 (female, male half and half) is planted, if a dosage group 6000mg/kg, after empty stomach 16h hour, by the disposable per os gavage of 0.2mL/10g body weight by Switzerland of 18 ~ 22g.After gavage, Continuous Observation 14 days animal poisoning manifestations and death toll, calculate LD
50.Result shows, and nanometer disinfection emulsion is to chmice acute Oral toxicity test LD
50>5000mg/kg body weight, belongs to actual nontoxic level.
(2) Skin Irritation Test
Select body weight to be the rabbit 4 of 2.2 ~ 3.0kg, male and female are not limit.24h before test, cuts the hair of back part of animal both sides, scope 3cm
2.Tested material stock solution 0.2mL drips in 2.5cm by next day
22 layers of gauze of size are applied ointment or plaster at side skin surface, then covers with the non-stimulated oilpaper of one deck, then use non-stimulated immobilization with adhesive tape.Opposite side is as blank (or solvent control).Effect 4h, then washes away residual tested material with warm water; Respectively at 1h, 24h, 48h after removal tested material, observe local skin reaction, mark.Result shows, and this emulsion acts on rabbit skin 4h, does not cause animal skin irritant reaction, and skin irritation index <0.4, stimulus intensity belongs to nonirritant.
(3) eye irritant test
Select body weight be 2.0 ~ 3.0 rabbit 4, male and female are not limit.Get stock solution 1-2 to drip, in instillation rabbit one branch hole conjunctival sac, another branch hole is as normal control; Drip the passive closed 4s of eye after tested material, use normal saline flushing 5min.After eye drip, 1h, 24h, 48h, 4d, 7d detect by an unaided eye the damage of rabbit eyes conjunctiva, iris and cornea and recovery situation.Result shows, and this emulsion is up to 0 to Rabbits with Acute eye irritation score index, and stimulus intensity belongs to nonirritant.
(4) micronucleus test
Switzerland is selected to plant Kunming outbred mice 50 (female, hero half and half), body weight 25-30g.Female, male Mus is divided all at random in 5 groups.According to the acute toxicity tests, 4 test group press the tested material stock solution gavage of body weight 5000mg/kg, 2500mg/kg, 1000mg/kg dosage respectively, negative control distilled water gavage, positive control cyclophosphamide (40mg/kg) gavage.Gavage 2 times, 24 hours, interval, after the 2nd gavage, 6h draws materials, conventional film-making microscopy.Statistical procedures is carried out with U inspection.Result show, this emulsion to PCEMNR without causing micronucleus effect.
This toxicity test result shows, and nanometer composite skin disinfection emulsion provided by the invention is safe, nontoxic, non-stimulated, can use safely at human body skin, mucosa multipath and have protective effect.
Claims (8)
1. a nanometer composite skin disinfection emulsion, it is made up of double-chain quaternary ammonium salt compounds, inorganic nano antiseptic material, emulsifying agent, Aloe extraction solution and water;
Described double-chain quaternary ammonium salt compounds is two (dodecyl dimethyl) ethylene diammonium of two decyl dimethyl ammonium bromide, double-track suspended self-propelled conveyor or bromination;
Described inorganic nano antiseptic material is nano zine oxide, nano phase ag_2 o or nano titanium oxide;
Described Aloe extraction solution is aloe stoste.
2. nanometer composite skin disinfection emulsion according to claim 1, it is characterized in that: described emulsifying agent is made up of three normal-butyl phosphate, sorbitol anhydride monopalmitate and polyoxyethylenesorbitan sorbitan monooleate, volume ratio is 1:(2 ~ 3): (1 ~ 3).
3. nanometer composite skin disinfection emulsion according to claim 1 and 2, is characterized in that: in described nanometer composite skin disinfection emulsion:
The mass body volume concentrations of described double-chain quaternary ammonium salt compounds is 1 ~ 5g/L;
The mass body volume concentrations of described inorganic nano antiseptic material is 0.1 ~ 0.5g/L, and particle diameter is 50 ~ 100nm;
The volume fraction of described emulsifying agent is 10% ~ 20%;
The volume fraction of described Aloe extraction solution is 20% ~ 30%;
Surplus is water.
4. the nano combined disinfection emulsion according to any one of claim 1-3, is characterized in that: in described nano combined disinfection emulsion, and the diameter of drop is 10 ~ 30nm.
5. a preparation method for the nanometer composite skin disinfection emulsion according to any one of claim 1-4, comprises the steps:, by after each component mixing any one of claim 1-4, can obtain described nanometer composite skin disinfection emulsion through high pressure homogenize.
6. preparation method according to claim 5, is characterized in that: described high pressure homogenize, at 25 ~ 35Mpa, carries out under the condition of 20 ~ 30 DEG C.
7. nanometer composite skin disinfection emulsion according to any one of claim 1-4 is in skin degerming or the application prepared in disinfectant liquid for skin.
8. application according to claim 7, is characterized in that: described disinfectant liquid for skin all kills staphylococcus aureus, escherichia coli and Candida albicans.
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| CN201510221366.6A Active CN104815096B (en) | 2015-05-04 | 2015-05-04 | A kind of nanometer composite skin disinfection emulsion and preparation method thereof |
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| CN111166763A (en) * | 2020-02-17 | 2020-05-19 | 吴强 | Bactericidal bacteriostatic agent |
| CN112716822A (en) * | 2021-02-03 | 2021-04-30 | 深圳市南门科技有限公司 | Disinfectant for resisting new coronavirus and preparation method thereof |
| CN114767712A (en) * | 2022-04-25 | 2022-07-22 | 杭州西子卫生消毒药械有限公司 | Low-volatility disinfectant and preparation method thereof |
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| CN111166763A (en) * | 2020-02-17 | 2020-05-19 | 吴强 | Bactericidal bacteriostatic agent |
| CN112716822A (en) * | 2021-02-03 | 2021-04-30 | 深圳市南门科技有限公司 | Disinfectant for resisting new coronavirus and preparation method thereof |
| CN114767712A (en) * | 2022-04-25 | 2022-07-22 | 杭州西子卫生消毒药械有限公司 | Low-volatility disinfectant and preparation method thereof |
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| CN104815096B (en) | 2019-10-11 |
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