KR101319997B1 - Cosmetic composition containing extract of Epilobium angustifolium Linne and Adina rubella Hance for improving skin wrinkle - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles containing pink needle (Epilobium angustifolium Linne) extract and Adina rubella Hance extract as an active ingredient. In the present invention, the mixed extract of the pink needle flower extract and the bead flower extract shows a greatly improved synergistic effect in the wrinkle improvement effect, it is excellent in promoting collagen production in the skin, inhibitory effect of MMP-1 production and elastase inhibitory activity It can be used as a cosmetic composition for improving wrinkles.

Description

Cosmetic composition for extracting wrinkles containing pink needle and beech extract as an active ingredient {Cosmetic composition containing extract of Epilobium angustifolium Linne and Adina rubella Hance for improving skin wrinkle}

The present invention relates to a cosmetic composition for improving wrinkles containing pink needles and bead extract, specifically, the effect of promoting collagen production, containing MMP-1 (matrix metalloproteinase-1) The present invention relates to a cosmetic composition for anti-wrinkle having excellent production inhibitory effect and elastase inhibitory activity.

The function of cosmetics is not only clean and aesthetic, but also free radicals and free radicals caused by external factors such as moisture, pollutants and stress, ultraviolet rays, and internal factors such as deterioration of immune cells and deterioration of cell activity. It has been extended to remove or suppress the cause of skin aging such as protein, nucleic acid, and cell membrane lipid destruction by peroxides. Among them, cosmetics for wrinkle improvement and suppression have received much attention from consumers who want to maintain youth.

Wrinkles are formed by repetitive movements of muscles in a specific direction over long periods of time, depending on age, external environment, and UV radiation. Damage to skin cells caused by these factors causes various aging phenomena. Collagen and elastin, which constitute the dermal matrix, are not easily produced and degraded. Thus, the firmness of the matrix is reduced and the elasticity of the skin is reduced and wrinkles are generated.

Meanwhile, elastase is an enzyme that degrades elastin, an insoluble elastic fiber protein of animal connective tissue, and hydrolyzes all proteins including proteins that support and form connective tissues such as collagen and elastin. Elastase proteases have been identified in platelets, mononuclear leukocytes, macrophages, and smooth muscle cells, in addition to multinuclear leukocytes and pancreas. Leukocyte elastase belongs to the serine proteinase. That is, the nucleophilic group where the serine residue at position 195 in the primary sequence of the enzyme attacks the a-carbonyl of the valine or alanine of the substrate. Acts as. Fibroblast elastases, on the other hand, belong to metalloproteinases that contain metal ions in their active sites. Some of the elastase activity of multinucleated leukocytes may be useful in vivo, such as complement and lymphocyte activation, but excessive elastase activity is detrimental to the skin.

The mechanism of photoaging is known to decrease collagen synthesis by UV irradiation and increase the expression of matrix metalloproteinase, which leads to increased degradation of collagen and elastin, which are the major constituents of the substrate, and thus photoaging including wrinkles. have. Elastase is also an enzyme that decomposes elastic substances during the decomposition of elastic fibers, and its activity is increased by ultraviolet irradiation, which is involved in photoaging.

Recently, many studies have been published to improve skin wrinkles using natural materials. Korean Patent Laid-Open Publication No. 2011-0024657 discloses a cosmetic composition for improving wrinkles containing a bottle pine (Callistemon lanceolatus) extract, Korean Patent Publication No. 2011-0110069 skin whitening and wrinkles comprising a ginger tree leaf extract An improved cosmetic composition is disclosed, and Korean Patent Publication No. 2011-0120813 discloses a cosmetic composition for improving wrinkles containing Euphorbia supina extract as an active ingredient.

Therefore, the present inventors screened herbal medicines effective in improving wrinkles, and as a result, a mixed extract of pink needles and bead trees having a specific compounding ratio exerted a synergistic effect, inhibiting the expression of elastase, and promoting collagen biosynthesis. It was confirmed that the wrinkles are improved and the present invention was completed.

It is an object of the present invention to provide a cosmetic composition containing pink needles and bead flower extracts, which has an effect of improving skin wrinkles. In particular, it is an object of the present invention to provide a cosmetic composition that improves wrinkles by inhibiting the activity of elastase, inhibiting MMP-1 production and promoting biosynthesis of collagen.

According to the present invention to achieve the above object, there is provided a cosmetic composition for improving wrinkles containing a mixture of pink needles and bead flower. The mixed extract is pink needle extract and bead extract is mixed in a weight ratio of 1:10 ~ 10: 1, preferably pink needle extract and bead extract is mixed in a weight ratio of 1: 1.

The pink needle and bead flower extract is contained 0.005 to 50% by weight, preferably 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The mixed extract improves wrinkles by promoting collagen production in the skin. In addition, the mixed extract has a feature of improving skin wrinkles by inhibiting MMP-1 production in the skin and inhibiting the activity of elastase.

The mixed extract is nanoencapsulated, microencapsulated or nanosomes can be applied as a raw material in the form of a solution or powder.

In addition, the cosmetic composition containing pink needle extract and bead extract is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, It may be applied in any one formulation selected from the group consisting of essences, packs, soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders and eye shadows.

Cosmetic composition containing pink needles and bead extract is effective in improving skin wrinkles, in particular, elastase inhibitory effect, MMP-1 production inhibitory effect and collagen biosynthesis promoting effect. The cosmetic composition containing the pink needle and bead extract is useful for the anti-aging cosmetic composition through the wrinkle improvement effect.

The present invention relates to a cosmetic for wrinkle improvement using the collagen production promotion, MMP-1 production inhibition and elastase inhibitory effect of pink needles and bead extract.

Pink Needle Flower (Epilobium angustifolium Linne) is a perennial herb that is also a perennial herbaceous flower. It is a plant that grows. The pink needle flower is about 1.5m high, and the stem of the ground extends to the side and sprouts from the end to form a colony. Leaves are lanceolate, 8 ~ 15cm long, 1 ~ 3cm wide, with pointed ends, fine teeth on the edges, and white on the back. In June-August, the end of the main stem has a genus inflorescence and reddish purple flowers. Po is linear and has a peduncle of 0.8-3cm in length. The calyx leaves and the petals are 4 each, the stamen is 8, the pistil is 1, and short and curved hairs are densely formed in the ovary. Fruits are 8-10cm long, ripen in October, and seeds have tubular hair. The outpost is called erythema, the root is nau, and the leaves contain ursolic acid, oleanolic acid, nonacosane, hexacosnol, etc. The red pectoris has the effect of lowering and lattice, and is used for the treatment of weak swelling, diarrhea, indigestion due to indigestion, and scrotum swelling. , Graft (접) is effective.

Adina rubella Hance is a deciduous shrub of Rubiaceae, also called red algae, and grows in the mountains below 700m above sea level in Jeju Island. The height is about 3 ~ 4m, the stem is straight and the branches are extended a lot. The leaves are single leaves, the surface of the leaves is green, and the back side is light green with dense hairs. Flowers are head-shaped in yellow or white at the end of branches in July-August. Flowers are long and fruit is ripe in October. The leaves and stems of the bee tree are called weeping hawk (水 楊梅), and the roots are called weeping root (水 楊梅 根) and contain ursolic acid (olesolic acid) and oleanolic acid (oleanolic acid). Falcons are effective for clearing fever and detoxification, and are used for the treatment of high fever diarrhea, toothache, eczema and traumatic bleeding. Used for the treatment of heat and wastewater.

In the cosmetic composition of the present invention, pink needles and bead flower extract is composed of a weight ratio of 1:10 to 10: 1, preferably a weight ratio of 1: 1.

The pink needle and beech extract as an active ingredient is contained 0.005 to 50% by weight, more preferably 0.01 to 20% by weight based on the total weight of the composition. If it is contained below 0.005% by weight, the activity is weak, and when it exceeds 50% by weight, it is unstable in the formulation.

The pink needle and the bead mixture extract of the present invention inhibits the activity of elastase, an enzyme of elastin in the skin, and acts to inhibit MMP-1 production. It also has a mechanism of action to improve skin wrinkles by promoting collagen biosynthesis.

The pink needle extract and bead extract of the present invention can be prepared as follows. Wash out the pink needle flowers, stems, leaves, and flowers of the beech tree, and dry them in the shade. The dried product is placed in an extraction container and extracted for a predetermined time at an appropriate temperature in an appropriate amount of the extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a solvent selected from water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, chloroform, hexane, acetonitrile, or a mixed solvent thereof may be used. Preferably, hydrothermal extraction is performed by adding ethanol. It is characterized by. The amount of the extraction solvent is 1 to 15 times, preferably 7 to 8 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as hot water extraction, reflux cooling extraction, ultrasonic extraction, etc., and may be extracted once to five times, preferably as an extraction method of hot water extraction.

The deposition temperature is maintained at about 40 ~ 70 ℃, more preferably at 60 ℃ is most effective in the prevention of rot, discoloration and odor. The deposition time is preferably maintained for 2 to 7 hours, preferably 3 hours.

The extract obtained by the deposition is left for 1 to 3 days, preferably 1 day, and then filtered using a filter cloth and filter paper and dried with a rotary decompression evaporator to correspond to extracts of pink needle fruit and leaves and branches and bead extract. Get powder to make.

The optimal mixing ratio for mixing the pink needle extract and bead extract of the present invention is that the pink needle extract and bead extract are mixed in a weight ratio of 1:10 to 10: 1, more preferably 1: 1. On the other hand, the mixed extract of the present invention may be extracted after mixing the pink needle flower and bead, or may be mixed after each extract.

If present in the form of a solution or powder in the form of nanocapsules, microcapsules, nanosomes, etc. containing the pink needle extract and bead extract, those skilled in the art will know that the amount of the solution or powder in the composition according to the invention You will be able to adjust

Cosmetics made of a cosmetic composition containing the present invention pink needles and bead extract as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible lotion. In addition, cosmetics containing the pink needle and beech extract of the present invention can be prepared in the form of adjuvants that can be applied topically or systemically as commonly used in the field of dermatology by containing a dermatologically acceptable medium or base. .

In addition, the cosmetic composition of the present invention, in addition to the pink needle and beetle mixture extract, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances , Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological arts, such as any other ingredients commonly used in the art. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

The cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, a moisturizing cream, a hand cream, , Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, eye shadow and the like.

[Example]

Hereinafter, the present invention will be described in detail with reference to the following examples and test examples, but the present invention is not limited to these examples.

Manufacturing example  One: Pink Needle Flower  Preparation of extract

After drying the dried pink needles in the shade, 1 kg of the ground powder was refluxed for 6 hours with a 10-fold 70% (V / V) ethanol aqueous solution, cooled down, and filtered through a filter paper having a permeate size of 8 μm. . The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and dried completely using a reduced pressure dryer to obtain 28 g of pink needle extract.

Manufacturing example  2: Bead  Preparation of extract

Crush the stems, leaves, and flowers of dried beech trees in the shade, and extract 1 kg of the mixture at a weight ratio of 1: 1: 1 with a 10-fold solution of 70% (V / V) ethanol for 6 hours. After cooling, the filtrate was filtered through a filter paper having a permeation size of 8 μm. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and completely dried using a reduced pressure dryer to obtain 17 g of a pink needle extract.

The dilution solution prepared by diluting the pink needle extract and bead extract prepared in Preparation Examples 1 and 2, and the mixture of 1: 1, respectively, in dimethyl sulfoxide for each concentration was used in the experiment.

Test Example  One : Elastase  Control activity test

10 ul of a solution prepared by mixing test sample and elastase enzyme solution (1,000 units / mL, Sigma, USA) with 2.5 wt% agar containing 0.1 wt% Elastin-Congo red (Sigma, USA) and reacting at room temperature for 10 minutes. After injection, the cells were incubated at 37 ° C. for 18 hours. After the incubation period, when the zona pellucida is formed around the Elastin-Congo red agar by the activity of the elastase, the effect of inhibiting the elastase activity may be measured by measuring the diameter of the halo.

Elastase alone is added dropwise to the agar medium containing elastin, while the solution of the concentration of the mixture of the pink needle and beet extract and the 1: 1 weight ratio of these extracts is 0.01, 0.05, 0.1 and 0.5% as a sample. Elastase inhibitory activity of pink needle extract was measured by measuring the diameter of halo on the medium by dropwise addition with elastase. As a control example, retinol crystal was added dropwise with elastase.

As a result of the test, the results shown in Table 1 were obtained.

sample
density
(%) Halo diameter (cm) Pink Needle Flower
extract Bead
extract Pink Needle Flower Extract
+
Bead Flower Extract Elastase Retinol crystal 0.01 1.5 1.6 1.2 1.8 0.9 0.05 1.4 1.4 1.1 0.10 1.1 1.1 0.9 0.50 1.1 1.1 0.9

The larger the halo diameter, the higher the degradation of elastin. As shown in Table 1 above, the halo diameter decreases when the pink needle extract, bead extract, and mixed extract thereof are treated. It can be seen that it exhibits an excellent effect of inhibiting elastase, and synergistic effect is obtained when the mixture of pink needle and beech flowers is treated as compared to the respective extracts.

Test Example  2: Collagen biosynthesis promoting effect test

Fibroblasts were inoculated in IMDM medium with 10% FBS at a cell concentration of 5 × 10 ⁵ and incubated in 37 ° C., 5% CO ₂ incubator for 24 hours. Incubated for 24 hours, the pink needle extract and bead extract prepared in Preparation Examples 1 and 2, and a mixture of 1: 1, respectively, were mixed in dimethyl sulfoxide so that the final concentration was 50, 100, 500, 1000 ㎍ / ml. Diluted solution prepared by diluting was added, and UV was irradiated for 1 hour after incubation under the same conditions for 18 hours to give stress to the cells. Thereafter, the fibroblasts were recovered using Trizol reagent (invitrogen, USA), mRNA was extracted, and cDNA was synthesized through a series of processes. The amount of gene expression was confirmed by electrophoresis by amplifying the PROCOLLAGEN TYPE I gene region from the synthesized cDNA, and gene amplification was performed using thermal cycler (GenePro, Hangzhou bioer tech., CHINA) using 10x taq polymerase buffer, 10 mM dNTP, 10 pmol primer (F: AGC CAG CAG ATC GAG AAC AT, R: TCT TGT CCT TGG GGT TCT TG), mixed with taq polymerase 2 minutes / 72 degrees 1 minute 28 cycles amplification, was carried out under the conditions of reacting for 5 minutes at 72 degrees. The expression rate of the produced procollagen was calculated according to the following equation. As a control, normal fibroblasts without treatment of the sample and UV irradiation were used.

Procollagen Expression (%) = B / A × 100 (%)

A: amount of procollagen expression in the control group

B: Procollagen Expression Levels of Sample Treatment and UV Irradiated Fibroblasts

As a result of the test, the results shown in Table 2 were obtained.

Sample concentration
(占 퐂 / ml) procollagen expression rate (%) Pink Needle Flower Extract Bead Flower Extract Pink Needle Flower Extract +
Bead Flower Extract 0 2.65 2.65 2.65 50 26.57 19.32 43.38 100 49.34 38.94 59.41 500 82.85 72.68 98.93 1000 119.23 105.04 143.10

As shown in the results of Table 2, the samples applied by mixing pink needles and bead extracts had higher collagen biosynthesis promoting effects related to skin wrinkles than when the pink needles and bead extracts were applied alone. Excellent synergy effect was shown.

Test Example  3: MMP -1 production inhibitory effect

Fibroblasts (Korean Cell Line Bank, Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc. Denmark) to 1st 10 ⁶ cells per well, and treated with DMEM medium (Sigma, USA) and 37 ° C. After incubating for 24 hours at the pink needle extract and bead extract prepared in Preparation Examples 1 and 2, and a mixture of 1: 1, respectively, so that the final concentration is 50, 100, 500, 1000 ㎍ / ㎖ Diluted solution prepared by diluting with dimethyl sulfoxide was added, and then cultured in serum-free DMEM medium for 48 hours. After the incubation, the supernatant of each well was collected and the amount (쨉 g / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) And the results are shown in Table 3 below. At this time, TGF-β (10 mg / ml, Roche, United States) was used as a positive control of MMP-1 production inhibition rate.

Sample concentration
(占 퐂 / ml) Inhibition rate of MMP-1 production (%) Pink Needle Flower
extract Bead
extract Pink Needle Flower Extract + Bead Flower Extract TGF-beta 50 17.5 14.6 31.4 42.3 100 24.3 26.9 60.1 65.5 500 48.1 39.1 68.6 69.7 1000 57.6 48.2 86.2 88.9

As shown in the results of Table 3, the sample applied by mixing pink needles and bead extract was higher MMP-1 production inhibitory effect than the case of applying the pink needles and bead extract alone, the synergistic effect more excellent Showed a similar effect to the control TGF-β.

Test Example  4: cytotoxicity test

Fibroblasts were inoculated in IMDM medium with 10% FBS at a cell concentration of 5 × 10 ⁵ and incubated for 24 hours in a 37 ° C., 5% CO 2 incubator. After incubation, the medium was removed, and the pink needle extract and bead extract prepared in Preparation Examples 1 and 2, and the mixture of 1: 1, respectively, were mixed so that the final concentration was 50, 100, 500, 1000 ㎍ / ml. The diluted solution prepared by diluting the seed was incubated for 24 hours, followed by MTT (3- [4,5-dimethylthiazol-2yl] -2,5-diphenyltetra

zolium boromide, Sigma, USA) solution was added to 100 μl of each well (3mg / ㎖) and further incubated for 4 hours. After removing the supernatant, 150 μl of dimethylsulfoxide was added, and the resulting formazan was shaken by shaking for 30 minutes. Absorption was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max 190). Cell viability was calculated according to the following formula and the results are shown in Table 4 below.

Cell viability (%) = absorbance of the sample addition group / absorbance of the control group × 100

Sample concentration
(占 퐂 / ml) Cell survival rate (%) Pink Needle Flower Extract Bead Flower Extract Pink Needle Flower Extract +
Bead Flower Extract 0 100 100 100 50 100 100 100 100 100 100 100 500 100 100 100 1000 94.88 95.35 95.23

As shown in the results of Table 4, all of the samples applied by mixing pink needle extract, bead extract, and pink needle and beet extract were found to be non-cytotoxic at a concentration of 500 µg / ml, and the concentration was 1000 µg / ml. The cell viability was reduced in ml, but it was confirmed that there is no problem in safety.

Example  One: emulsion  Base manufacturing

Emulsion base was prepared according to the composition of Table 5 below for clinical trials on the skin wrinkle improvement effect and skin safety of the cosmetic containing the pink needle extract and bead flower extract of the present invention.

Comparative Example  1, 2: emulsion  Base manufacturing

To prepare a emulsion base containing only pink needle extract or no extract of the present invention as shown in the composition of Table 5 as a comparative example.

ingredient weight% Example 1 Comparative Example 1 Comparative Example 2 glycerin 3.00 3.00 3.00 Disodium iodide 0.02 0.02 0.02 Polyglyceryl-3-methylglucoside distearate 1.50 1.50 1.50 Cetearyl alcohol 0.50 0.50 0.50 Caprylic / capric triglyceride 7.00 7.00 7.00 Polyacrylamide & C13-14 Isoparaffin & Laureth-7 0.60 0.60 0.60 Pink Needle Flower Extract 1.00 1.00 - Bead Flower Extract 1.00 - - Incense, preservative a very small amount a very small amount a very small amount Purified water Balance Balance Balance system 100 100 100

Test Example  5: skin wrinkle improvement

Skin wrinkle improvement effect of the cosmetic composition containing the pink needle flower and bead extract of the present invention was measured in 30 healthy women aged 35 to 50 years. First, it was divided into three groups, Example 1 in Group A, Comparative Example 1 in Group B, and the formulation of Comparative Example 2 in Group C were applied twice a day (morning and evening) on the face for 3 months. After 3 months, the degree of wrinkle improvement was evaluated by subject's questionnaire and image analysis of wrinkles.

The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in Table 6 below.

Image analysis of wrinkles was performed by taking a replica of the eye under the eye before the experiment was started, and replicas immediately after the experiment was completed at the same area under the eye. Was measured. The measurement results of wrinkle density by image analysis are shown in Table 7 below as a reduction rate for wrinkle density compared to before use.

sample No improvement Slight improvement Moderate improvement A significant improvement Example 1 One One 4 4 Comparative Example 1 2 3 4 One Comparative Example 2 6 4 - -

sample Wrinkle Density Reduction (%) Example 1 50 Comparative Example 1 34 Comparative Example 2 15

From Tables 6 and 7, it can be seen that the skin wrinkle improvement effect of Example 1 containing the pink needle and bead flower extract is very excellent.

Test Example  6: skin safety test

In order to confirm the skin safety of the cosmetic composition containing the pink needle extract and bead extract of the present invention, there has never been a hypersensitivity reaction to skin irritation in the past, and 20 women in their 20s to 30s without skin disease or skin allergy symptoms Human skin patch test was conducted. The test site was first wiped with 70% ethanol and then dried. The materials of Example 1 and Comparative Examples 1 and 2 were added dropwise into the Fin chamber (Finn chamber, 100x 10, EPITEST, Finland) by 15 µg, and then sealed in the forearm inner part of the test subject. The patch was patched for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. The skin response was determined according to the regulations of the Internatiolnal Contact Dermaitis Research Group (ICDRG), and the average response rate was calculated according to the following formula, and the results are shown in Table 8. Evaluation criteria and scores of skin response are shown in Table 9.

Average skin reactivity = (score × number of responses × 100 × 1/2) × [3 (maximum score) × total number of subjects (n)]

Test substance After 1 hour 24 hours later Response (%)
(n = 20) ± + ++ ± + ++ Example 1 - - - - - - 0.00 Comparative Example 1 - - - - - - 0.00 Comparative Example 2 - - - - - - 0.00

Symbol score Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One The border is clear but weak erythema, edema and palsy ++ 2 Clear erythema, papules and small follicles +++ 3 Severe erythema and alveoli, scab formation

As can be seen from Table 8, there was no skin irritation in the cosmetic containing pink needles and bead extract, and thus skin stability was excellent.

Test Example  7: Stability Test of Formulation

The stability test for the cosmetic containing the pink needle extract and bead extract of the present invention was carried out by the following method. In order to test the stability of the cosmetics Example 1 and Comparative Examples 1 and 2 were kept in an opaque glass container in a constant temperature bath at a constant temperature of 45 ℃ stored for 12 hours and kept in a completely shaded refrigerator kept at 4 ℃ constant After storing for 12 weeks in an opaque glass container, discoloration and odor, and the degree of separation was measured and compared the results are shown in Table 10 below. At this time, the product discoloration, odor and separation degree was classified into six grades and evaluated.

0: No change 1: Very little separation (color change, odor)

2: little separation (discoloration, discoloration) 3: slight separation (discoloration, discoloration)

4: Severe separation (discoloration, odor) 5: Extremely severe separation (discoloration, odor)

division Stability of formulation Example 1 Comparative Example 1 Comparative Example 2 45 ° C 4 ℃ 45 ° C 4 ℃ 45 ° C 4 ℃ discoloration 0 0 0 0 0 0 Deodorant 0 0 0 0 0 0 detach 0 0 0 0 0 0

As can be seen from Table 10, Examples 1 and Comparative Examples 1 and 2 were stable at almost 45 ° C. and 4 ° C. without discoloration, discoloration or separation, and pink needle extract and bead extract were stable in the formulation. It exists as a state.

Cosmetic composition containing pink needles and bead extract of the present invention can be prepared in the following various formulations.

Example  2: flexible lotion

A flexible lotion containing pink needles and bead extract with the composition shown in Table 11 below was prepared by a conventional method.

ingredient Content (% by weight) Pink Needle Flower Extract 0.5 Bead Flower Extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl vinyl polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  3: nutrition lotion ( Milk lotion )

To a nutritive lotion containing pink needles and bead extract with the composition shown in Table 12 was prepared by a conventional method.

ingredient Content (% by weight) Pink Needle Flower Extract 0.6 Bead Flower Extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia nut oil 2.5 Polysorbate 20 1.6 Sorbitan sesquiterate 1.6 Propylparaben 0.6 Carboxyl vinyl polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  4: nutrition cream

A nutritious cream containing pink needles and bead extract with the composition shown in Table 13 was prepared in a conventional manner.

ingredient Content (% by weight) Pink Needle Flower Extract 1.0 Bead Flower Extract 1.0 Pro-type glycerin monostearate 2.0 Stearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Purified vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Preservative, fragrance, pigment a very small amount Purified water Balance Sum 100

Example  5: Massage  cream

A massage cream containing pink needles and bead extract with the composition shown in Table 14 was prepared by a conventional method.

ingredient Content (% by weight) Pink Needle Flower Extract 0.5 Bead Flower Extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  6: Pack

A pack containing pink needles and bead extract with the composition described in Table 15 below was prepared in a conventional manner.

ingredient Content (% by weight) Pink Needle Flower Extract 0.5 Bead Flower Extract 0.5 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 glycerin 4.5 Carbopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Claims (7)

Wrinkle improvement cosmetic composition containing pink needles and bead flower extract as an active ingredient. According to claim 1, wherein the mixed extract pink needle flower extract and bead flower extract is a cosmetic composition for improving wrinkles, characterized in that consisting of a weight ratio of 1:10 ~ 10: 1. According to claim 1, wherein the mixed extract is wrinkles cosmetic composition for improving wrinkles, characterized in that nanoencapsulated, microencapsulated or nanosomes. The cosmetic composition for improving wrinkles according to claim 1, wherein the composition improves wrinkles by promoting collagen production. The cosmetic composition for improving wrinkles according to claim 1, wherein the composition improves wrinkles by inhibiting elastase inhibitory activity and inhibiting the production of matrix metalloproteinase-1 (MMP-1). The cosmetic composition for improving wrinkles according to any one of claims 1 to 5, wherein the pink needle and beech mixed extract is contained in an amount of 0.005 to 50% by weight based on the total weight of the composition. The composition according to any one of claims 1 to 5, wherein the composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream Wrinkle improvement, characterized in that consisting of any one selected from the group consisting of essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow Cosmetic composition for.