KR101928386B1

KR101928386B1 - Cream type cosmetic composition having skin tone correcting, UV protecting, anti-wrinkle and whitening effect, and preparing method of the same

Info

Publication number: KR101928386B1
Application number: KR1020160022270A
Authority: KR
Inventors: 김란
Original assignee: 원광보건대학교 산학협력단
Priority date: 2016-02-25
Filing date: 2016-02-25
Publication date: 2018-12-12
Also published as: KR20170100124A

Abstract

One embodiment of the present invention is a method for preparing a pharmaceutical composition comprising, as an active ingredient, an active ingredient selected from the group consisting of golden, yellow, white, mung bean and licorice mixed extract water, Huangchil leaf, mixed water extract of Baeksoooojimo, germanium powder, adenosine, Naacinamide, A cosmetic composition having a skin color correction and ultraviolet shielding function, which comprises a skin color correction and ultraviolet ray blocking component, a skin softening agent, an emulsifier, an increasing agent, a moisturizer, an additive and purified water in a predetermined ratio and a manufacturing method thereof The cream form cosmetics manufactured according to the embodiment of the present invention have a skin color correction and ultraviolet cutoff function and can smooth and soften the skin texture according to the inclusion of the active ingredients and can be used for moisturizing, , Elasticity enhancement, and the like.

Description

[0001] The present invention relates to a cosmetic composition having a skin color correcting function, an ultraviolet screening function, a wrinkle improving function and a whitening function,

More particularly, the present invention relates to a cosmetic composition containing cream, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, , Adenosine, neacinamide, and chestnut extract as an active ingredient, and a method for producing the same.

The destruction of the ozone layer is accelerating due to environmental pollution, and the desire to protect the skin from ultraviolet rays is increasing day by day as the outdoor leisure activity population of skiing and golfing is increased.

Although ultraviolet light can promote the differentiation of skin epidermal cells, it may be beneficial to strengthen the skin barrier. However, when exposed continuously, it may cause burn, skin blackening, erythema, collagen destruction, pigmentation and wrinkling, have.

In general, sunscreens can be classified as physical sunscreens and chemical sunscreens. The physical ultraviolet screening agent is a principle that blocks ultraviolet rays from penetrating into the skin by scattering ultraviolet rays, which has a disadvantage of being excellent in blocking effect and less irritating but opaque and causing white turbidity phenomenon. In addition, the chemical sunscreen agent is a principle that inhibits ultraviolet penetration into the skin by using a substance that absorbs ultraviolet rays. Unlike a physical ultraviolet screener, the chemical ultraviolet screener does not cause whitening due to transparency. However, There is a problem that skin irritation such as contact dermatitis and allergy is caused when the blending amount is increased.

Accordingly, in order to solve the above-mentioned problems, a sunscreen agent is manufactured by appropriately blending the components of a physical sunscreen agent and a chemical sunscreen agent. In addition, various functions are added in accordance with consumers' interest and desire for skin aesthetics Research and development of ultraviolet screening agents are actively under way.

In this regard, Korea Patent No. 10-1216410 (the name of the invention: skin-true ultraviolet-shielding functional cosmetic composition containing the extract of Stone-on-the-leaved leaf and phyllotaxin extract, hereinafter referred to as Prior Art 1) 0.1 to 30.0% by weight of the extract from the outer layer of the stalk extract and 0.1 to 30.0% by weight of the extract of the root extract extracted from the root of the skin using ethanol, Discloses a technique relating to a blocking functional cosmetic composition.

In this connection, Korean Patent No. 10-1477891 (name of the invention: highly functional cosmetic composition containing whitening, wrinkle, anti-aging and anti-aging cosmetic ingredient from sattan plant ethanol extract, hereinafter referred to as prior art 2) Discloses a technique for a high-performance cosmetic composition comprising a sycamore tree obtained by adding water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, ethyl acetate, chloroform, n-butanol and 1,3-butylene glycol have.

KR 10-1216410 KR 10-1477891

The above-mentioned prior art 1 discloses a technique relating to an ultraviolet barrier functional cosmetic composition which contains an extract of a stone-on-the-leaf and an extract of a callus root and has an excellent skin soothing effect. However, the present invention can be applied to various cosmetic compositions with various functions such as pigmentation, aging, It does not meet the desire of consumers to solve complex skin improvement effects.

The prior art 2 discloses a technique relating to a high-functional cosmetic composition having functions of whitening, wrinkling, anti-aging, ultraviolet ray blocking and the like, including a sycameria tree ethanol extract inhibiting the activity of tyrosinase, The present inventors have confirmed only the inhibitory effect of the ethanol extract on tyrosinase activity, but do not disclose a specific preparation example of a cosmetic composition having an ultraviolet blocking function including the ethanol extract of sutureus tree.

SUMMARY OF THE INVENTION Accordingly, the present invention has been made in view of the above problems, and an object of the present invention is to provide a cosmetic composition containing ultraviolet screening and skin color correcting components to protect the skin from ultraviolet rays and having skin care effects.

More specifically, the present invention relates to a cream containing a functional material having various effects such as skin moisturizing, whitening, wrinkle improvement, elasticity enhancement, and antioxidation as an active ingredient and having a skin color correction and ultraviolet shielding function It is an object of the present invention to provide a technique relating to a mixed composition of a cosmetic formulation.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

In order to accomplish the above object, an embodiment of the present invention provides a method of extracting mixed extracts of golden, yellowtail, yellowtail, mung bean, licorice, mixed hullchill leaf, chrysotile, germanium, germanium powder, adenosine, A cosmetic composition having a skin color correction and an ultraviolet shielding function as an active ingredient.

In an embodiment of the present invention, a mixed composition of a cream-form cosmetic having a skin color correction and an ultraviolet shielding function comprises 3 to 10 wt% of the effective ingredient; Skin color correction and ultraviolet blocking component 8 to 15 wt%; 10.5 to 38 wt% of a skin-softening agent; 3.5 to 6.5 wt% emulsifier; From 4.5 to 11 wt%; 5 to 15 wt% of a moisturizer; 0.5 to 10.5 wt% of an additive; And purified water to make the total blend composition 100 wt%.

In an embodiment of the present invention, the skin color correction and ultraviolet ray blocking component may include crystalline inorganic mineral, titanium dioxide, mica, yellow iron oxide, red iron oxide, black iron oxide, and hexylsilmethoxycinnamate.

In an embodiment of the present invention, the skin-softening agent is selected from cyclopentasiloxane, isohexadecane, cyclohexasiloxane, caprylic / capric triglyceride, dimethicone, sweet almond oil, grapeseed oil, macadamia seed oil And may include one or more species.

In an embodiment of the present invention, the emulsifier may be at least one selected from sorbitan sesquioleate, sertilpyridine / piperidine-10/1 dimethicone, hydrogenated lecithin and phage-10 dimethicone crosspolymer . &Lt; / RTI >

In an embodiment of the present invention, the thickener may include at least one selected from xanthan gum, sodium chloride, dimethicone / vinyl dimethicone crosspolymer, and methyl methacrylate crosspolymer.

In an embodiment of the present invention, the humectant may include at least one selected from glycerin, diglycerin, propanediol, dipropylene glycol, butylene glycol, sorbitol, hexylene glycol, and polyethylene glycol.

In an embodiment of the present invention, the additive may include at least one selected from an emulsifying stabilizer, a dispersion stabilizer, a preservative, a binder, a film forming agent, an antioxidant and a perfume.

In order to accomplish the above object, another embodiment of the present invention is to provide a method of extracting mixed extracts of golden, yellowtail, yellowtail, mung bean, licorice, mixed hullgrass, chrysotile, germanium, germanium powder, adenosine, The present invention also provides a method for producing a mixed composition of a cream cosmetic product having a skin color correction function and an ultraviolet shielding function, which comprises an extract as an active ingredient.

In the examples of the present invention, the mixed composition of the cosmetic preparation having the skin color correction and the ultraviolet ray shielding function is mixed with a mixture of golden, yellow, white, mung bean, licorice mixed extract, A first step of mixing the extract, adenosine, niacinamide, a moisturizing agent, and purified water at 70 to 90 ° C with stirring to prepare a supernatant mixture; A third step of mixing the water-based mixture and the oil-based mixture at 70 to 90 DEG C with stirring to prepare an oil-water mixture, lowering the temperature of the oil-water mixture to 30 to 45 DEG C A skin color correction and an ultraviolet shielding component, and stirring and dispersing the mixture. After the mixture from the fourth step is defoamed, 20 Support can be prepared by a fifth step of aging at a temperature of 25 ℃.

According to an embodiment of the present invention, a skin softening agent and a moisturizing agent that can suppress skin aging caused by ultraviolet rays by correcting skin color and blocking ultraviolet rays, including skin color correction and ultraviolet shielding components, And can bring about skin softening and moisturizing effect. By including various herbal extracts, antioxidant, anti-inflammation, antioxidant effect and whitening, wrinkle improvement and the like can be brought about.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

Hereinafter, embodiments of the present invention will be described in detail. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as " comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The present invention relates to a skin color correction and ultraviolet screening function comprising an extract of a mixture of golden, yellow, white, mung bean and licorice mixed extracts, Huangchil leaf, Bacillus subtilis, germanium powder mixed extract, adenosine, The present invention relates to a cosmetic composition comprising a cream composition comprising 3 to 10 wt% of the active ingredient, 8 to 15 wt% of a skin color correction and ultraviolet shielding ingredient, 10 to 38 wt% of a skin softening agent, 3.5 to 6.5 wt% 4.5 to 11 wt%, a humectant of 5 to 15 wt%, an additive of 0.5 to 10.5 wt%, and purified water. BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with respect to components constituting a mixed composition of a cream cosmetic composition having a skin color correction and an ultraviolet shielding function according to the present invention.

First, the active ingredient of the present invention will be described.

In the present invention, in the present invention, the mixed extracts of golden, yellow, white, green, and licorice extracts have antibacterial and anti-inflammatory actions as main functions, but are not limited thereto and have functions of whitening, elasticity enhancement, antioxidation and wrinkle improvement. Hereinafter, the efficacy and effects of the respective constituent materials constituting the mixed-extracted water will be described in detail.

In the present invention, gold is a medicinal plant, and its roots are used as one of herbicides, diuretic, branch, insect and anti-inflammatory agents. The golden extract is extracted from the root of gold and can act as a anti-inflammatory, antibacterial, anti-allergic, antioxidant, whitening and moisturizing function and can be used as a raw material for skin cosmetics. In the present invention, Rhizoma belongs to the perennial plant and is known to contain active ingredients such as berberine, coptisine, worenine and palmatine.

The extract of Huangryun extract is extracted from the roots of Huangryun. It suppresses the activity to prevent the external harmful substances from proliferating on the skin surface, suppresses the hypersensitivity reaction of the skin caused by the harmful substances, and protects against the deterioration and decay of cosmetic products Can be used as an antiseptic.

The extract is extracted from the bark of an acacia tree. It contains berberine, which is an alkaloid. It inhibits the activity and growth of substances harmful to the skin to help skin care, To protect the skin.

The mung bean extract can be obtained by steam distillation of mung bean seeds. It contains palmic acid, stearic acid, linoleic acid, vitamins, flavonoids and saponin components. In addition to antibacterial and anti-inflammatory actions, And it is known to have the effect of improving elasticity.

The licorice extract inhibits the substances that are harmful to the skin from exhibiting activity, inhibits the hypersensitivity reaction of the skin, suppresses the oxidation reaction of the skin caused by the active oxygen which is the cause of skin aging, and contributes to enhance the elasticity of the skin.

In the present invention, the mixed extract of Hwangchilji leaf, Baeksoo, Geumo, and germanium powder has effects such as antioxidation, antibacterial, anti-inflammation, elasticity enhancement, and whitening of pores. Hereinafter, the specific efficacy and effects of each composition material constituting the mixed extract will be described.

In the present invention, the extract of Huangchil leaf is a component extracted from the leaves and stems of Huangchu tree belonging to the evergreen tree of Phalaenopsis phalaenopsis, and it is known as a medicinal plant having excellent antibacterial and anti-inflammatory action. It can improve the elasticity and moisturizing effect, and can inhibit the activity of tyrosinase that produces melanin, which can help skin whitening.

In the present invention, white water is one of the medicinal plants that have been cultivated for a long time as a vine plant belonging to a vine perennial herbaceous plant, whose roots extend long to the side and have round root roots. In addition to antioxidant, antibacterial and anti-inflammatory functions, the components extracted from the roots of Bacillus subtilis can enhance the skin's immunity and contribute to the improvement of elasticity, thereby improving wrinkles and whitening.

In the present invention, Zygo extract is extracted from rootstocks of Zygomycetes belonging to the Lily family. Boripyrin, which is a component derived from Zygomycota extract, is excellent in stability and excellent in skin elasticity due to its active ingredient, It is known to be used as a material. In addition, it is known that the boripyrin contained in the gum extract has excellent whitening effect and skin regeneration effect.

The germanium powder used in the present invention is harmless to the human body when it is used in an appropriate amount and is known to promote physiological activity such as promoting oxygen supply in the human body cells. The germanium powder adsorbs wastes containing sebum in the skin surface and pores, And a small amount of germanium absorbed in the skin can bring about an effect of improving the elasticity and wrinkling of the skin by the activating enzyme.

In the present invention, adenosine binds adenine and ribose components, and is present in a small amount in all organisms such as plants, plants and microorganisms. It is a wrinkle-improving ingredient certified by a pharmacist and promotes the synthesis of proteins in the dermal layer of skin, Thereby improving wrinkles, improving elasticity, and lifting the skin. In order to perform the above function, adenosine should be contained at 0.04 wt% or more based on 100 parts by weight of the total mixed composition.

In the present invention, niacinamide is a complex of water-soluble vitamin B, which is also called nicotinic acid and niacin. Melanocytes, which are skin-inducing components of melanin, are produced by tyrosinase in the basal layer, which is the lowest layer of the epidermal layer. To move to the stratum corneum close to the surface of the skin and cause pigmentation. Niacinamide can inhibit pigmentation by inhibiting this movement, and can lighten the pigment deposited on the stratum corneum to make skin whitening effect. In addition, niacinamide may contribute to the prevention of moisture loss in the skin by increasing the synthesis of intercellular quality. In addition, it has an antioxidant effect that suppresses active oxygen which is a cause of skin aging, and also has an effect of improving elasticity and improving wrinkles in addition to whitening function. In order for the niacinamide to exhibit the above effect, it should contain at least 2 wt% based on 100 parts by weight of the mixed composition of whole nutrition cream. However, it is known that when the amount of niacinamide is excessively increased, it causes eye creasing. For this reason, it may be preferable that niacinamide is contained in an amount of 2 to 3 wt%.

In the present invention, the Cryptomeria japonica extract is a component extracted from the genus Chrysanthemum belonging to the soybean family as early as the beginning of the month of the herb, which is also called as a herb extract, a safflower seed, a crusher, Cole seeds are known to have effects such as topical and anti-inflammatory effects. The outpost is a medicinal plant rich in resources used to treat skin diseases such as trauma bleeding and shingles. In addition, the outpost of the wooly owl contains trigonelline and canavanine, and the seed contains canaline, canavanine, homoserin, coumestrol, and the like . In addition, the outpast of Cubuntus is called Safflower seed, the seed is Cinnabon seed and it is used for medicinal purposes. The Safflower straw is collected from March to April and contains trigonelline, choline and adenine, , And seeds such as cannarin, canavanine, and homoserine are known to have an effect of helping blood circulation and brightening the eyes. The present inventors confirmed the skin aging inhibition, whitening effect, and antioxidative effect of the extract of Perilla vulgaris Seed Extract through the skin physiological activity test of the Perilla seed extract, and applied it as an effective ingredient of cosmetics.

Hereinafter, the cosmetic composition of the cream formulated together with the active ingredient will be described.

In the present invention, the skin color correction and ultraviolet shielding component comprises crystalline inorganic mineral, titanium dioxide, mica, yellow iron oxide (Cl 77492), red iron oxide (Cl 77491), black iron oxide (Cl 77499), and hexyl hexamethoxycinnamate . The crystalline inorganic mineral may be at least one selected from minerals such as alumina, ruby, sapphire and corundum, but it is not limited thereto. In one embodiment of the present invention, it may be preferable that the skin color correction and ultraviolet shielding ingredients are contained in an amount of 8 to 15 wt% based on 100 wt% of the total mixed composition. When the content of the skin color correction and ultraviolet shielding ingredients is less than 8 wt% It may not be suitable for skin color correction and ultraviolet ray blocking functional cosmetic, and when the skin color correction and ultraviolet ray blocking component are an inorganic pigment component except for ethylhexylmethoxycinnamate, when the content exceeds 15 wt%, the pigment content is relatively increased It may be difficult to produce cosmetics having a uniform composition, and it may be undesirable because it contains more skin color correction components than necessary.

In the present invention, the skin-softening agent includes at least one selected from cyclopentasiloxane, isohexadecane, cyclohexasiloxane, caprylic / capric triglyceride, dimethicone, sweet almond oil, grape seed oil and macadamia seed oil . The skin-softening agent may be contained in an amount of 10.5 to 38 wt% based on 100 wt% of the total mixed composition. When the content of the skin-softening agent is more than 38 wt%, the content of the oil component is relatively increased to require a relatively large amount of an emulsifier And it may be difficult to ensure the stability of the product, such as occurrence of phase separation, which may be undesirable.

In the present invention, the thickening agent is used to lower the flowability and increase the usability of the mixed composition by increasing the viscosity of the mixed composition, and is selected from the group consisting of xanthan gum, sodium chloride, dimethicone / vinyl dimethicone crosspolymer and methyl methacrylate crosspolymer But it should not be construed as being limited thereto. In the present invention, the thickener may be contained in an amount of 4.5 to 11 wt% based on 100 wt% of the premix composition. If the content of the thickener is less than 4.5 wt%, the flow of the product may be high, When the content exceeds 11 wt%, the viscosity may excessively increase, which may be undesirable.

In addition, the mixed composition of cosmetics having the skin color correction and ultraviolet shielding function according to the present invention may contain a moisturizing agent for the purpose of enhancing and sustaining the moisturizing effect. The humectant may be glycerin, diglycerin, propanediol, dipropylene glycol , Butylene glycol, sorbitol, hexylene glycol, and polyethylene glycol, but is not limited thereto. The content of the moisturizing agent may be 5 to 15 wt% based on 100 wt% of the total mixed composition. If the content of the moisturizing agent is less than 5 wt%, the moisturizing effect and the duration of moisturizing may not be sufficient. %, The content of the water phase component increases, and it may be difficult to mix with the oil phase component in the production of the mixed composition.

The cosmetic composition for skin color correction and ultraviolet ray shielding according to the present invention may contain an additive in an amount of 0.5 to 10.5 wt% based on 100 wt% of the total composition. The additive may be an emulsifying stabilizer, a dispersion stabilizer, a preservative, An antioxidant, and a flavoring agent.

Hereinafter, a method for preparing a mixed composition having a skin color correction function and an ultraviolet blocking function according to an embodiment of the present invention will be described.

In the examples of the present invention, the mixed composition of the cosmetic preparation having the skin color correction and the ultraviolet ray shielding function is mixed with a mixture of golden, yellow, white, mung bean, licorice mixed extract, A first step of mixing the extract, adenosine, niacinamide, a moisturizing agent and purified water at 70 to 90 ° C with stirring to prepare a water-based mixture; a step of extracting the Cow's milk extract, skin-softening agent, emulsifier and oil- A third step of mixing the water-based mixture and the oil-based mixture at 70 to 90 DEG C with stirring to prepare an oil-water mixture, lowering the temperature of the oil-water mixture to 30 to 45 DEG C, A skin color correction and an ultraviolet ray blocking component are added and stirred and dispersed; a step of defoaming the mixture from the fourth step; It can be prepared by a fifth step of aging at a temperature of 25 ℃.

More preferably, the cosmetic composition having a skin color correction and ultraviolet shielding function comprises 0.06 to 1.4 wt% of mixed extracts of golden, yellow, white, mung bean, and licorice, mixed extracts of Huangchil leaf, Baishuo, Wherein the active ingredient is at least one selected from the group consisting of 0.5 to 2.5 wt% of adenosine, 0.04 to 0.1 wt% of adenosine, 2 to 3 wt% of niacinamide, and 0.4 to 3 wt% , Skin color correction comprising red iron oxide and black iron oxide and 5 to 15 wt% of an ultraviolet barrier component, 3 to 10 wt% of cyclopentasiloxane, 0.1 to 0.5 wt% of isohexadecane, 4 to 12 wt% of cyclohexasiloxane, 0.2 to 2 wt% of fricic-triglyceride, 2.5 to 7.5 wt% of dimethicone, 0.5 to 4 wt% of sweet almond oil, 0.1 to 1 wt% of grape seed oil, % Of an emulsifier, 0.1 to 1 wt.% Of a dispersion stabilizer, 0.11 to 0.55 wt.% Of a preservative, 0.14 to 1.5 wt.% Of a thickening agent, 0.1 to 1 wt.% Of a skin softener, 5 to 15 wt.% Of a humectant, 3.5 to 6.5 wt. 0.05 to 2 wt% of a film forming agent, 0.05 to 2 wt% of an antioxidant, 0.5 to 2.05 wt% of a perfume, and the total weight of the pre-composition is 100 wt%. Hereinafter, a method for preparing a cream cosmetic composition having a skin color correction and ultraviolet shielding function according to the present invention will be described in detail with reference to the following Production Examples.

Hereinafter, Preparation Examples and Experimental Examples of the present invention will be described.

[ Experimental Example 1] Coleoptera seed extract Skin physiological activity Experiment

1. Measurement of cytotoxicity and survival rate by MTT assay

Cell viability was measured by the method of Carmichael et al. (Cancer Res 1987; 47 (4): 936-942). Raw 264.7 mouse macrophage cells were cultured in DMEM medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% streptomycin under 5% carbon dioxide at 37 ° C. Cells were plated in 96-well plates at a concentration of 1 × 10 ⁴ cells / well in an amount of 100 μl each. After culturing in an incubator for 24 hours, each extract was treated with 10 μl of each concentration and cultured for another 24 hours. MTT solution was added to each well and reacted for another 4 hours. Then, the supernatant was removed to remove the formazan formed on the bottom of the well, and 100 μl of dimethylsulfoxide was added. After 30 minutes, the absorbance at 540 nm (Power Wave X340, BIO-TEK). The average value was obtained by repeating the experiment six times for each concentration of 12.5 mg / ml, 25 mg / ml, and 50 mg / ml of Coleoptera seed extract. The relative cell viability was determined by taking the absorbance of the control group without the extract as 100% Respectively.

The toxicity of Coleus koreanum Seed Extract to Raw 264.7 cells was measured by MTT analysis and found that 91.54 ± 6.24%, 103.00 ± 5.22%, and 96.61 ± 7.56% of the extracts were obtained at 12.5 mg / ㎖ and 25 mg / Cell survival rate.

As a result of the MMT analysis, 103% cell proliferation effect was observed at a concentration of 25 mg / ml of the extract, and 96.61% cell survival rate even at a concentration of 50 mg / ml. These values are shown in the following table: Cell survival rate of snake strawberry fruit is 50-80%, cell survival rate is about 80% at 500ppm of ethanol and acetone extract, and 60% of cell survival rate at 500ppm concentration of water extract and vitamin C. , It can be concluded that the extract of Coleoptera japonica has little effect on cytotoxicity.

2. The DPPH radical Scatters Measure

DPPH radical scavenging activity was examined to investigate the antioxidative effect of the extract of Coleoptera syrup. Skin is aged by physiological aging and photoaging, and free radicals, which are active oxygen species in the cells, cause deformation of biomolecules, leading to aging. Therefore, in order to delay skin aging, an ability to erase free radicals generated in the body is required.

The radical scavenging activity of the black currant seed extract was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl, Sigma) as the method of Blois (Nature 1958; 26: 1199-1120). 10 μl of the sample solution dissolved in methanol and 190 μl of the DPPH solution (300 μM) were mixed and allowed to stand in a dark room for 15 minutes, and the absorbance (Power Wave X340, BIO-TEK) was measured at 517 nm. The experiment was repeated six times at concentrations of 2.5 mg / ml, 5 mg / ml and 10 mg / ml in the control and experimental groups, respectively. The scavenging activity was calculated on the basis of the absorbance of the reaction solution mixed with 10 μl of methanol instead of the sample solution, and ascorbic acid, which is an antioxidant in the body, was used as a positive control. The DPPH radical scavenging ability of the perilla seed extract was 63.52 ± 2.86% at 2.5 mg / ml, 90.71 ± 0.65% at 5.0 mg / ml and 91.6 ± 0.21% at 10.0 mg / ml, In the case of acetic acid, DPPH radical scavenging activity was 93.56 ± 0.07% at 5.0 mg / ml, 93.72 ± 0.04% at 5.0 mg / ml and 93.71 ± 0.05% at 10.0 mg / And it was confirmed that it is similar to corbic acid. There was statistically significant difference between scavenging ability and ascorbic acid scavenging ability at each concentration (p <0.05).

In addition, DPPH radical scavenging activity was found to have a high concentration-dependent effect on the extract of Coleoptera sieboldii. In particular, it exhibited a DPPH radical scavenging activity of 90% or more at a concentration of 5.0 to 10.0 mg / ml, and this value shows almost similar antioxidative effects as DPPH radical scavenging activity of ascorbic acid as a control group.

3. Measurement of Elastase Inhibitory Activity of Curd Leaf Extract

Elastase inhibitory activity was measured by the method of Cannell et al. (Plaanta Med 1988; 54 (1): 10-14). Human leukocyte elastase was dissolved in 50 mM sodium acetate buffer solution (pH 5.3) to prepare 1 unit / ml solution. Para-nitroanilide was dissolved in dimethyl sulfoxide to prepare a 20 mM stock solution. 20 μl of elastase and 20 μl of the sample were dispensed into a 48-well plate, and the plate was washed with 10 mM sodium phosphate buffer pH 6.8) was added 200 μl of 400 μM para-nitroanilide. The reaction solution was incubated at 37 ° C for 20 minutes, and 120 μl of the reaction solution was transferred to a 96-well plate and the absorbance (Power Wave X340, BIO-TEK) was measured at 410 nm. Repeated experiments were repeated 5 times at concentrations of 25 ㎍ / ㎖, 50 ㎍ / ㎖, 100 ㎍ / ㎖ and 250 ㎍ / ㎖ in the control and experimental groups, respectively. Quercetin was used as a positive control, and the inhibitory activity of elastase was calculated according to the following formula (1).

[Equation 1]

Inhibitory activity (%) of elastase = {1- (S-B) / C} * 100

Where B is the absorbance of the reaction solution to which 10 mM sodium phosphate buffer solution (pH 6.8) is added instead of the enzyme, and C is the absorbance of the reaction solution containing 10 mM sodium phosphate buffer (pH 6.8 ) Is added to the reaction solution.

The elasthase inhibitory activity of the sesame seed extract was 73.33 ± 1.58% at 25 μg / ml, 80.25 ± 2.07% at 50 μg / ml, 90.82 ± 2.46% at 100 μg / ml, 97.36 ± 1.69% at 250 μg / The activity of quercetin in the control group was 75.60 ± 1.69% at 25 μg / ml, 79.12 ± 1.03% at 50 μg / ml, 85.03 ± 1.03% at 100 μg / ml and 105.28 ± 3.63% at 250 μg / Lt; / RTI >

Elastase is an enzyme that degrades elastin, and by this action it is known to be the main enzyme for reducing elasticity and wrinkling by cutting the network structure of the dermis. As a result of the measurement of the inhibitory activity of the elastase, the inhibitory activity was 90.82% at 100 μg / ml of the curd seed extract, and this value was 100 μg / ml as compared with quercetin, a flavonoid-based elastase inhibitor, (P <0.05). The inhibition of elastase activity of Coleoptera seed extracts was significantly higher than that of the control group (p <0.05).

4. Measurement of inhibitory activity of MMP-1 (collagenase)

It is known that when the content of collagen is reduced by the increase of collagen decomposition by the expression of MMP such as collagen (collagen) degrading enzyme by ultraviolet rays, skin elasticity is reduced and wrinkles appear. The collagenase inhibitory activity test is for measuring the degree of reduction of skin aging by measuring the inhibitory activity of collagenase which hydrolyzes collagen which plays a supporting role of skin.

MMP-1 inhibitory activity was measured by the method of Sim et al. (J Soc Comet Scientists 2004; 30: 439-444). 300 콜 of collagen solution (0.25 mg / ml) dissolved in PBS (pH 6.0), 600 시 of sample and 600 콜 of collagenase solution (0.5 unit) were added. After 20 minutes at room temperature, the fluorescence value was measured using a fluorescence spectrophotometer (Hitachi) at an absorption wavelength of 280 nm and an emission wavelength of 300 nm. In the control group, PBS (Phosphate Buffer Saline) was added in the same amount instead of the sample solution. The mean values were used for each of the concentrations of 50 ㎍ / ㎖, 100 ㎍ / ㎖, 250 ㎍ / ㎖ and 500 ㎍ / ㎖ in the control and experimental groups, respectively. The quercetin inhibiting activity of collagen denaturation The inhibitory activity of collagenase was calculated according to the following formula (2).

&Quot; (2) "

Inhibitory activity of collagenase (%) = {(C-S) / C} * 100

Where S is the absorbance of the sample and enzyme-containing reaction solution, and C is the fluorescence of the reaction solution to which PBS is added in place of the sample.

The inhibitory activity of MMP-1 on CMP was 23.00 ± 0.06% at 50 μg / ml, 43.9 ± 0.06% at 100 μg / ml, 76.9 ± 0.02% at 500 μg / ml at 250 μg / The inhibitory activity of quercetin was 95.24 ± 0.03%, and that of the control group was 69.95 ± 0.12% at 100 μg / ml, 84.2 ± 0.09% at 100 μg / ml, 98.4 ± 0.02% at 500 μg / 99.95 ± 0.01%, respectively, and the collagenase inhibitory activity of the vernal seed extracts was significantly lower than that of the control group, quercetin (p <0.05).

However, the MMP-1 inhibitory activity of Cuneiform Seed Extract showed a high concentration-dependent effect, and in comparison with Coenzyme Q 10, which is effective for prevention of skin aging and cell regeneration, showed inhibitory activity of 1% to 91.1% The extract of Perilla seeds showed 95.24% at 500 ㎍ / ㎖, suggesting that the inhibitory activity of MMP-1 is superior.

5. Measurement of inhibitory activity of tyrosinase of Coleoptera seed extract

The inhibition activity of tyrosinase was examined by measuring the enzyme inhibitory activity of tyrosinase, which is involved in the production of melanin, which is a cause of abnormal pigment formation, and examines the whitening effect of the black currant seed extract.

Tyrosinase inhibitory activity was measured using the method of Mason et al. (Biochim. Biophys Acta 1965; 111: 134-146). 220 μl of a 0.1 M phosphate buffer solution (pH 6.5), and 20 μl of a sample dissolved in a 0.1 M phosphate buffer solution (pH 6.5) were mixed. 20 mu l of mushroom tyrosinase (1,000 units / ml) was dispensed into the mixed solution, and the mixture was reacted at 37 DEG C for 30 minutes. The amount of dopachrome produced by the reaction was calculated by measuring absorbance (Power Wave X340, BIO-TEK) at 490 nm. Repeated experiments were repeated three times at concentrations of 6.25 mg / ml, 12.5 mg / ml, 25 mg / ml, and 50 mg / ml in the control and experimental groups, and the average value was used to inhibit melanin production. Reducing ascorbic acid was used as a positive control, and the inhibitory activity of tyrosinase was calculated according to the following equation (3).

&Quot; (3) "

Inhibitory activity (%) of tyrosinase = {(C-S) / C} * 100

Where C is the absorbance of the reaction solution to which PBS is added instead of the sample, and S is the absorbance of the reaction solution containing the sample.

The tyrosinase inhibitory activity of Coleoptera seed extract was activated in a concentration dependent manner. The inhibitory activity was 61.47 ± 2.85% at 6.25 mg / ml, 73.65 ± 1.47% at 12.5 mg / ml, 89.41 ± 5.77% at 25 mg / ml and 94.41 ± 9.15% at 50 mg / The inhibitory activity of the free acids was 68.81 ± 0.29% at 6.25 mg / ㎖, 88.75 ± 1.58% at 12.5 mg / ㎖, 90.18 ± 0.44% at 25 mg / ml and 89.99 ± 0.29% at 50 mg / ml. It was significantly lower at 6.25 mg / ㎖ and 12.5 mg / ㎖, but higher at 50 ㎎ / ㎖ (p <0.05) than the control group of ascorbic acid, which is a representative whitening substance.

[ Manufacturing example One]

1. Preparation of Chrysanthemum morifolium extract

The mixture was extracted three times with 80 wt% of acetone at room temperature of 22 to 23 째 C, filtered, and then concentrated under reduced pressure. The concentrated extracts were lyophilized to complete the production of chestnut milk extract.

2. Golden, goldthread , Yellowish white , Mung bean and licorice

The extract was extracted with distilled water to make 5wt% of mixed powder of dried golden, yellow, white, mung bean and licorice.

3. Hwangchil , Baewooo Oh , Germanium and germanium powder

4 wt% of Hwangchil leaf powder, 4 wt% of Baekgoo powder, 4 wt% of Rhizoma roots, and 3 wt% of germanium fine powder were weighed and put into the disinfected cotton bag with the above weighed herb and germanium powder and loosely bundled. A cotton pouch containing the mixed powder was put in a far-infrared ray extractor containing 42 wt% of 1,3-butylene glycol as a moisturizing ingredient and 43 wt% of distilled water, and the mixture was sufficiently submerged and then maintained at a temperature of 42 to 45 ° C And extracted for 72 hours.

4. Skin color correction And UV protection functional cosmetics

A cream form cosmetic having a skin color correction and an ultraviolet shielding function was prepared with the composition shown in Table 1 below. Specifically, the ingredients shown in the following Table 1 were classified into a water phase, a first oil phase, a second oil phase and a floating phase, and the raw materials of each phase were weighed and mixed. After mixing the mixture of the first oil phase and the second oil phase, the mixture was heated to 80 DEG C and stirred evenly, and the mixture of water phase was evenly mixed at 80 DEG C. [ The temperature of the water-liquid mixture was lowered to 40 ° C, and the flotation mixture was added and the temperature was maintained and stirred until evenly mixed. When a mixture of the desired cream formulation was formed, the cream was formulated into a cream form cosmetic having skin color correction and ultraviolet shielding function by degreasing and aging at room temperature (22 to 23 degrees) for 7 days.

Ingredients Function (secondary function) Content [wt%] Classification of raw materials Purified water solvent 37.63

Awards

Xanthan gum Increasing agent (skin-softening agent) 0.100 Sodium chloride Increasing agent (neutralizing agent) 1,000 1,2-hexanediol Preservative (moisturizer) 1,000 Magnesium aluminum silicate Increasing agent (binder) 0.500 Golden, Yellow, White, Mung bean, Licorice Antibacterial, anti-inflammatory, antioxidant 0.500 Hwangchiljyeol, Baeksoo, Geomo, germanium powder mixed extract Antibacterial, anti-inflammatory, antioxidant 1.500 glycerin Moisturizer 2.000 Butylene glycol Moisturizer 5.000 Tocopheryl acetate Antioxidant 0.200 Disodium iodide Preservative (metal ion sequestrant) 0.030 Adenosine wrinkle improvement 0.040 Awards
Niacinamide Whitening 2.000 To a solution of < RTI ID = 0.0 & Sunscreen 0.500

First charge
Sorbitan sesquioleate Emulsifier 0.800 Three tilipies / piperazine-10/1 dimethicone Emulsifier (skin-softening agent) 2.000 Hydrogenated lecithin Emulsifier 0.800 10-dimethicone crosspolymer Emulsifier (thickener) 1,000 Dimethicone / Vinyl Dimethicone Crosspolymer Increasing agent (skin-softening agent) 6.300 Polyvinylpyrrolidone Film forming agent (humectant) 0.200 Polymethylsilsesquioxane Dispersion stabilizer 0.320 Synthetic sapphire Skin color correction, UV protection 1.500 Cyclopentasiloxane Skin softening agent 7.000
The second charge
Isohexadecane Skin softening agent 0.100 Cyclohexasiloxane Skin softening agent 9.000 Caprylic / capric triglyceride Skin softening agent 0.500

The second charge Dimethicone Skin softening agent 5.000 Sweet almond oil Skin softening agent 2.000 Grape seed oil Skin softening agent 0.300 Macadamia seed oil Skin softening agent 0.100 Chestnut extract Whitening Anti-wrinkle 1,000 Heptyl undecylate Binders (skin-softening agents) 0.300 Phenoxyethanol, an ethylhexyl glycerin mixture Preservative 0.310 A mixture of titanium dioxide, methyl methacrylate crosspolymer, mica, yellow iron oxide, red iron oxide, black iron oxide, distatti phosphate, triethoxycaprylylsilane

UV protection, skin color correction

9.070

injury Spices 0.400 Sum 100

[ Experimental Example 2] Skin color correction And stability evaluation of functional cosmetics with UV protection

The skin color correction and UV-blocking functional cosmetics prepared in Preparation Example 1 were evaluated for stability. The manufactured skin color correction and ultraviolet barrier functional cosmetics were stored at 25 ° C for 30 days. As a result of examination of whether or not the image was separated, the odor and the color were altered, it was confirmed that the separation of the image and the alteration did not occur.

[ Experimental Example 3] Skin color correction And UV-Visible Functional Cosmetics

In order to evaluate the usability of cosmetic products for skin color correction and ultraviolet barrier function according to Production Example 1 of the present invention, blind tests were conducted on 11 women aged 22 to 56 years. (Comparative Example) and B (Preparation Example 1) were put in an empty container, respectively, and the product of S company and the cosmetic product of Production Example 1 according to the present invention were respectively placed in an empty container. Respectively. In the blind test, 0.1 g of each of the cosmetics contained in the A and B containers was applied to the face, hand-applied, and a satisfactory degree of satisfaction with the spreadability, spreadability, and adhesion was measured and the results are shown in Table 2.

division Production Example 1 Comparative Example Difficult to distinguish Brittle 9 2 Spreadability 6 5 Adhesiveness 5 5 One Skin color correcting power 11 vitality 9 2 Finish feeling (effect of moistness, smoothness, etc.) 8 2 One

As shown in Table 2, cosmetics having skin color correction and ultraviolet shielding function manufactured according to an embodiment of the present invention are highly evaluated in terms of spreadability, spreadability, skin color correcting power, durability, and finish feeling, It was confirmed that the evaluation performance was better than the comparative example.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims

Golden, yellow, white, mung bean, licorice mixed extract; Hwangchilji leaf, Baeksoo, Geumo, germanium powder mixed extract; Adenosine; Niacinamide; And Coleoptera seed extract as active ingredients,
The skin-softening agent and the skin-softening agent in an amount of 3 to 10 wt%, 3 to 10 wt% of the active ingredient, 8 to 15 wt% of a skin color correction and ultraviolet shielding component, 10.5 to 38 wt% of a skin softening agent, 3.5 to 6.5 wt% 10.5 wt%, and purified water to make the total mixed composition 100 wt%
The adenosine is contained in an amount of 0.04 wt% or more, the niacinamide is contained in an amount of 2 to 3 wt%
Wherein the skin-softening agent is selected from the group consisting of 3 to 10 wt% cyclopentasiloxane, 0.1 to 0.5 wt% isohexadecane, 4 to 12 wt% cyclohexasiloxane, 0.2 to 2 wt% caprylic / capric triglyceride, 2.5 to 7.5 wt% dimethicone 0.5 to 4 wt% of sweet almond oil, 0.1 to 1 wt% of grape seed oil, and 0.1 to 1 wt% of Madagami oil,
Wherein the additive comprises 0.1 to 1 wt% of a dispersion stabilizer, 0.11 to 0.55 wt% of a preservative, 0.14 to 2.9 wt% of a binder, 0.05 to 2 wt% of a film former, 0.05 to 2 wt% of an antioxidant, and 0.5 to 2.05 wt% A cosmetic composition comprising a cosmetic composition having a skin color correction, ultraviolet screening, wrinkle improvement and whitening function, which comprises a flavor.

delete

The method according to claim 1,
Wherein the skin color correction and ultraviolet ray blocking component comprises a crystalline inorganic mineral, titanium dioxide, mica, yellow iron oxide, red iron oxide, black iron oxide, and ethylhexylmethoxycinnamate. A cosmetic composition comprising a cream-form cosmetic having a whitening function.

delete

The method according to claim 1,
Wherein the emulsifier is at least one selected from the group consisting of sorbitan sesquioleate, cetylpiperazine / piperidine-10/1 dimethicone, hydrogenated lecithin and phage-10 dimethicone crosspolymer. A cosmetic composition containing a cream, a cosmetic having a function of correcting, ultraviolet blocking, wrinkle and whitening.

The method according to claim 1,
Wherein the thickening agent comprises at least one selected from xanthan gum, sodium chloride, dimethicone / vinyl dimethicone crosspolymer, and methyl methacrylate crosspolymer. &Lt; / RTI >

The method according to claim 1,
Wherein the humectant includes at least one selected from the group consisting of glycerin, diglycerin, propanediol, dipropylene glycol, butylene glycol, sorbitol, hexylene glycol, and polyethylene glycol. Functional cosmetic composition.

delete