KR102164246B1 - Cosmetic Compositions for Anti-oxidation and Protecting Skin from UV Comprising Complex Extracts of Plants - Google Patents

Abstract

The present invention relates to a cosmetic composition for antioxidation and skin protection against ultraviolet rays, comprising a plant complex extract of Limonia acidissima Linn., Angelica gigas Nakai, and Saposhnikovia divaricata Schiskin as an active ingredient. The extract provided from the present invention exhibits excellent DPPH scavenging activity and cell protecting activity against ultraviolet rays, and thus can be used as a cosmetic composition for antioxidation and skin protection against ultraviolet rays.

Description

Cosmetic Compositions for Anti-oxidation and Protecting Skin from UV Comprising Complex Extracts of Plants}

The present invention relates to a cosmetic composition for skin protection against antioxidants and ultraviolet rays comprising a plant complex extract as an active ingredient.

Reactive oxygen introduced from outside the body or generated in the body promotes aging in the body or may cause cancer. Therefore, research on antioxidants that inhibit oxidation by free radicals are being conducted, and antioxidants are known as phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium. However, when applied to the skin, antioxidants that exist in nature cannot be expected to have a substantial effect. On the other hand, synthetic antioxidants having excellent antioxidant power and low cost have a problem that their use is limited due to concerns about safety such as side effects on the human body.

In addition, moderate sunlight helps our body to produce vitamin D, and so on, but excessive exposure can accelerate skin aging and lead to health risks such as skin cancer. Ultraviolet rays irradiated from sunlight is a major cause of skin erythema, swelling, freckles, and skin cancer. Recently, many studies on various skin diseases caused by ultraviolet rays have been conducted. In general, ultraviolet rays are classified into ultraviolet A (320-400 nm), ultraviolet B (280-320 nm), and ultraviolet C (240-280 nm) according to their wavelength. Ultraviolet A is not absorbed by the ozone layer, reaches the earth's surface consistently throughout the year regardless of the weather, and has a relatively long wavelength so that it passes well through glass windows. In the skin, it is known to act as the main cause of skin blackening or photoaging by inducing skin aging and skin irritation, such as promoting skin cancer, wrinkles, and melanin formation by reaching the dermal layer. Ultraviolet B is mostly absorbed by the ozone layer, but some of it reaches the earth's surface and does not pass through windows. In the skin, it reaches only the epidermal layer and causes erythema, which corresponds to a burn when overexposed. Ultraviolet C is absorbed by the ozone layer and disappears without reaching the surface.

Recently, in order to suppress skin damage caused by such ultraviolet rays, a sunscreen agent that protects cells by blocking the penetration of ultraviolet rays itself, or a cosmetic composition using ingredients having an antioxidant function that suppresses the production of intracellular reactive oxygen species caused by ultraviolet rays. Development is actively taking place.

However, most of the developed skin protectants by UV rays are made of synthetic compounds as active ingredients, so they cause damage to the skin or cause side effects such as itchiness and spots.Therefore, there are no skin side effects, and a new natural product with excellent skin protection activity by UV rays. Materials need to be developed. In addition, the sun protection factor (SPF) of the sun protection cosmetic is expressed as a sun protection factor (SPF), which indicates the degree of preventing erythema from occurring due to UV rays, which mainly means UVB blocking effect. This is a situation where the development of a natural material that can effectively block UV A is required because there is a possibility that the product with high SPF is applied and exposed to sunlight, which has a relatively strong effect on skin blackening and aging.

Meanwhile, in accordance with the recent nature-oriented and environment-friendly consumption trend, natural products derived from plants or animals are also used in various forms based on their functionality.

Tanaka ( Limonia acidissima Linn.) belongs to the Rutaceae family and is a tropical plant commonly found in the Indian Peninsula (South Asia) and Southeast Asia. Tanaka has been widely used by Myanmar as a natural cosmetic product for skin care since 2,000 years ago, and as a home remedy. Myanmar people grind the bark of Tanaka with water and apply it to the face or the entire skin. This is because they believe that it has a cooling effect that cools the skin, softens the skin, removes acne and blemishes, and protects the skin from sunlight. to be. Tanaka is a traditional folk remedy and has been used for coughing, colds, appetite enhancement, thirst relief, constipation, vomiting, heartburn, and diarrhea suppression.

Angelica gigas Nakai is a perennial plant, and different species are cultivated in each country. In Korea, Angelica gigas Nakai, Chinese angelica ( A. sinensis (Oliv.) Diels), and Japanese angelica ( A. actriloba Kitagawa) are used for the same purpose. It is used as a herbal medicine. Among them, Chamdang-gwi is known to have a blood-replenishing effect that replenishes blood, and is prescribed as a blood-blood for anemia or women's diseases in oriental medicine. In addition, Angelica Angelica is known to be effective in preventing aging, improving wrinkles and tissue regeneration by helping blood circulation as it has an effect of improving blood flow.

Bangpung ( Saposhnikovia divaricata Schiskin.) is a perennial plant belonging to the Umbelliferae family, and is a medicinal plant called Jinbangpung, Sanbangpung, folding screen sprouts, Sanbangpung tree, and windbreak tree root. The windbreak grows in dry grasslands or foothills, and is distributed in Manchuria, China, Mongolia, and Siberia. In oriental medicine, windbreak has been used for the treatment of external feelings, headaches, carpals stiffness, windbreaks, fracture colic, limb pain relief, and tetanus for the purpose of presentation, geopung, sensation, and pain. In recent years, anti-inflammatory, anti-cancer, and anti-allergic effects have been reported as pharmacological effects of windbreak, and studies on skin inflammation have been actively conducted.

As a technology for a cosmetic composition for skin protection against antioxidant and UV rays using plant extracts, Korean Patent Publication No. 10-2019-0028996 ``For UV rays containing a complex extract of bitter gourd, persimmon leaves and silkworm as active ingredients. Cosmetic composition for skin protection", Korean Patent Publication No. 10-1959551 "A composition for improving skin hypopigmentation and protecting skin from ultraviolet rays containing applemint extract as an active ingredient", Korean Patent Publication No. 10-2015300 ``A cosmetic composition for skin protection against ultraviolet rays or ultraviolet rays absorbing or against ultraviolet rays containing a stingray extract'', Korean Patent Publication No. 10-1937162, ``a cosmetic composition for skin protection against ultraviolet rays containing seaweed extract as an active ingredient'', and the like are disclosed.

However, until now, using the plant complex extracts of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai and windbreak ( Saposhnikovia divaricata Schiskin), a cosmetic composition that can simultaneously provide antioxidant and UV-protective effects Research does not exist.

Thus, the present inventors have shown that the plant complex extracts of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai ( Angelica gigas Nakai) and windbreak ( Saposhnikovia divaricata Schiskin) exhibit excellent DPPH scavenging activity and cell protective activity against ultraviolet rays. The present invention was completed by discovering that it can simultaneously provide a skin protective effect.

Korean Patent Application Publication No. 10-2019-0028996 Korean Patent Publication No. 10-1959551 Republic of Korea Patent Publication No. 10-2015300 Korean Patent Publication No. 10-1937162

An object of the present invention is to provide a cosmetic composition for skin protection against antioxidants and ultraviolet rays derived from natural products.

Another object of the present invention is to provide a cosmetic product comprising the composition.

In order to achieve the above object, the present invention contains a plant complex extract of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai, and windbreak ( Saposhnikovia divaricata Schiskin) as an active ingredient for skin protection against antioxidant and ultraviolet rays. It provides a cosmetic composition.

In the present invention, the plant complex extract is characterized in that it is extracted with a solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof.

In the present invention, the plant complex extract is characterized in that it is extracted with ethanol.

In the present invention, the plant complex extract is characterized in that it is mixed in a weight ratio of 1:1 to 2000:1 to 2000 by using tanaka, angelica and windbreak as a dry weight.

In the present invention, the plant complex extract is characterized in that it contains 0.1 to 50% by weight based on the total weight of the composition.

In the present invention, the plant complex extract is characterized in that it has DPPH scavenging activity and cell protective activity against ultraviolet rays.

In the present invention, the ultraviolet ray is characterized in that the ultraviolet ray A.

In addition, the present invention provides a cosmetic comprising the composition.

Since the extract provided from the present invention has excellent DPPH scavenging activity and cell protective activity against ultraviolet rays, it can be used as a cosmetic composition for skin protection against antioxidant and ultraviolet rays.

1 is a graph showing the evaluation of free radical scavenging activity of Tanaka extract, Angelicae extract, Wind Poong extract and Tanaka, Angelica Angelica and the plant complex extract of Wind Poong.
Figure 2 is a graph showing the cytotoxicity evaluation of the plant complex extract of Tanaka, Angelica and Bangpung.
3 is a graph showing the evaluation of cytotoxicity of Tanaka extract, Angelicae extract, and windbreak extract.
Figure 4 is a graph showing the cell protective activity against ultraviolet A irradiation of Tanaka extract, Angelicae extract, windbreak extract, and Tanaka, Angelicae and windbreak plant complex extract.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by an expert skilled in the art to which the present invention belongs. In general, the nomenclature used in this specification is well known and commonly used in the art.

Throughout the specification of the present application, when a certain part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise stated.

According to one embodiment of the present invention, the present invention contains a plant complex extract of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai and windbreak ( Saposhnikovia divaricata Schiskin) as an active ingredient for skin protection against antioxidant and ultraviolet rays It relates to a cosmetic composition.

The extract according to the present invention may be obtained through various extraction methods known in the field to which the present invention belongs, and specifically, may be extracted with a solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof. Among them, extraction with ethanol is more preferable in terms of stably extracting the active ingredient.

The water may be alkaline water, and the organic solvent may be an anhydrous or aqueous lower alcohol having 1 to 4 carbon atoms, such as ethanol, propanol, methanol, n-butanol, acetone, 1,3-butylene glycol, etc. Organic solvent; Non-polar organic solvents such as ether, hexane, benzene, chloroform, and ethyl acetate; Vegetable organic solvents such as soybean oil and sesame oil; And it may include one or more of these mixtures. The organic solvent may be used alone or in combination of two or more.

As a solvent used in the extraction, it may be used as a mixed solvent including one or more organic solvents of ethanol, methanol, n-butanol, and propanol, and 1,3-butylene glycol or water in a solvent thereof. When the mixed solvent is used as the extraction solvent, the extraction efficiency of the active ingredient may be further improved. At this time, when using the mixed solvent, it may be appropriately mixed and used according to an arbitrary purpose.

Specifically, the content of the extraction solvent is 2 to 100 times the weight of the solid content of the plant complex of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai and windbreak ( Saposhnikovia divaricata Schiskin), more preferably 5 to 50 times, the most Preferably it may be 10 to 20 times.

In addition, the extract may be extracted by heating or at room temperature under conditions in which the active ingredient of the extract is not destroyed or the destruction is minimized. The extraction method is not particularly limited, and according to the type of the extract and the method of extraction, for example, hot water extraction, reflux cooling extraction, ultrasonic extraction, cold sediment extraction, and percolation extraction method may be used.

The number of extractions in the extracting step may be specifically repeated 1 to 5 times. In this case, the extraction efficiency of the active ingredient may be further improved.

For example, the step of extracting may include adding the extraction material and the extraction solvent to an extractor equipped with a reflux cooler, and then performing heating at 50 to 100°C for 4 to 20 hours with reflux cooling. At this time, the heating reflux cooling extraction method is a method of extracting by cooling after refluxing while heating, specifically, because vaporization may occur due to heating and solvent loss may occur, a cooling process is performed together to minimize solvent loss. Means the extraction method. Alternatively, in the step of extracting, the extraction solvent may be added to the extraction material, followed by immersion at 5 to 37° C. for 1 to 15 days, followed by cold precipitation.

In the step of extracting as another method, after adding the extraction solvent to the extraction material, percolation may be performed for 1 to 200 hours.

In another method, the extraction may be performed by adding an extraction solvent to the extraction material, followed by boiling at 50 to 100° C. for 0.5 to 72 hours.

In the step of extracting as another method, ethanol of 10 to 50 times the weight of the extracted material is added based on the dry solids of the plant complex, extracted for 1 to 72 hours at 20 to 80°C, filtered and concentrated to concentrate The extract in the state can be obtained.

In addition, the extraction material of the present invention includes not only the extraction material extracted by the above-described extraction solvent, but also the extraction material extracted through a conventional purification process. For example, the active fraction obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, is also included in the extract of the present invention.

Filtration is a process of removing suspended solid particles from the extract. The particles may be filtered using cotton, nylon, paper, or the like, or ultrafiltration, freezing filtration, centrifugation, etc. may be used, but is not limited thereto.

The step of concentrating the filtrate and then drying it includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of pulverizing the final dried extract may be added.

According to an embodiment of the present invention, the extract may be processed and used in various forms, such as an extract, a powder obtained by drying the extract, and a concentrate obtained by concentrating and filtering the extract. In addition, the extract is not particularly limited, but may be used in the form of, for example, tink, concentrate, extract, flow extract, etc. containing a hydrous alcohol as a leaching solvent.

According to one embodiment of the present invention, the plant complex extract is preferably made by mixing tanaka, angelica and windbreak as a dry weight in a weight ratio of 1:1 to 2000:1 to 2000, and 1:10 to 1000: It is more preferable that it is made by mixing in a weight ratio of 10 to 1000, and that made by mixing in a weight ratio of 1:50 to 500: 50 to 500 is most preferable in terms of improving both antioxidant and skin protective effects against ultraviolet rays. If it is out of the above range, it is not preferable because the skin protective effect may be reduced.

According to one embodiment of the present invention, the plant complex extracts of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai ( Angelica gigas Nakai) and windbreak ( Saposhnikovia divaricata Schiskin) obtained from the above method are 0.1 to 50 based on the total weight of the composition. It may be included in% by weight, more preferably 1 to 20% by weight. When the extract is less than 0.1% by weight based on the total weight of the composition, the function as a composition is deteriorated because the antioxidant and skin protection effect against ultraviolet rays cannot be sufficiently obtained, and when it exceeds 50% by weight, it is sufficient without containing more. Not only is it uneconomical because it can exhibit efficacy, and it is not preferable because it is difficult to use as a cosmetic because the stability and spreadability of the formulation are deteriorated.

Since the plant complex extract can simultaneously impart DPPH scavenging activity and cell protective activity against ultraviolet rays, not only skin beauty but also fundamental skin health improvement can be realized. In this case, the ultraviolet rays may be ultraviolet rays A.

As described above, since the plant complex extract has excellent antioxidant and cell protective activity against ultraviolet rays, it can be used as a cosmetic composition for skin protection against antioxidant and ultraviolet rays.

According to one embodiment of the present invention, the cosmetic composition is purified water (water), glycerin (glycerin), myristic acid (myristic acid), butylene glycol (butylene glycol), potassium hydroxide (potassium hydroxide), polyethylene Glycol (polyethylene glycol), stearate, stearic acid, lauric acid, lauramide DEA, glyceryl stearate, baehenyl alcohol ( behenyl alcohol), microcrystalline wax, phenoxyethanol, ethylhexylglycerin, tocopheryl acetate, 1,2-hexanediol, car Caprylyl glycol, hydroxyethylene cellulose, xanthan gum, collagen, sodium citrate, citric acid, magnesium ascorbate And it may include one or more selected from the group consisting of fragrance (fragrance).

In addition, the cosmetic composition of the present invention may be blended with other ingredients that are usually blended in the cosmetic, if necessary. For example, oil and fat components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators , A limiting agent or purified water is preferred, but is not limited thereto.

According to one embodiment of the present invention, when used as the cosmetic composition, skin ointment, cream, softening lotion, astringent lotion, nutrient lotion, pack, mask sheet, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment Ment, spray, gel, gel ointment, patch, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, cream, massage cream, nourishing cream, eye cream, moisture cream, hand cream, Foundation, nutritional essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion, and may have any one formulation selected from the group consisting of a body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, titanium oxide, etc. may be used as carrier components. I can.

When the formulation of the present invention is a body gel, in addition to the above-described active ingredients, components generally used in the manufacture of body gels, such as carbomer, triethanolamine, menthol, ethanol, glycerin, disodium EDTA, methylparaben, fragrance, etc. Can be used. Here, carbomer and triethanolamine act as a thickening agent, menthol acts as a fragrance ingredient, ethanol acts as a refreshing sensation, glycerin acts as a moisturizer, and disodium EDTA is a metal chelate. It functions as, and methylparaben is added to function as a preservative. In addition, in addition to menthol flavor, various flavors such as herbal flavor may be added to the fragrance.

In addition to the above-described specific ingredients, it is also within the scope of the present invention to apply known substances widely used in the art to achieve the functions of thickeners, cool sensations, fragrance ingredients, and moisturizers described above. Can be included.

When the formulation of the present invention is a powder or spray (aerosol), lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be added as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane/butane or dimethyl ether may be included.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is added as a carrier component, examples of which include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.

According to an embodiment of the present invention, a cosmetic composition for skin protection against antioxidant and ultraviolet rays may be prepared as a composition such as body gel, body lotion, body cream, body oil, etc. for promoting fat decomposition by adding conventional additives, It can also be manufactured in an aerosol type. In this case, the cosmetic composition is preferably used as a method of transdermal administration by directly applying or spraying on the skin.

According to one embodiment of the present invention, the amount of use of the cosmetic composition for skin protection against antioxidant and ultraviolet rays may be appropriately adjusted according to individual differences, formulations, and shapes such as age and skin fat condition, and skin against antioxidants and ultraviolet rays. It can be usefully used as a composition for a protective effect.

The composition obtained from the method as described above exhibits DPPH scavenging activity and cell protective activity against ultraviolet rays, thereby providing excellent antioxidants and cosmetics for skin protection against ultraviolet rays.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and that the scope of the present invention is not limited by these examples according to the gist of the present invention, for those of ordinary skill in the art to which the present invention belongs. It will be self-evident.

<Example 1> Preparation of plant complex extract

After mixing O.1g of Tanaka Herbal Powder (NH Market), 1g of dried Angelicae (Angelicae, Jecheon Herbal Herbs) and 1g of dried windbreak (Bangpung, Jecheon Herbal Herb), 100mL of purified water was added to The bath was extracted for 2 hours. After extraction, it was filtered through a 0.45㎛ filter to prepare a final plant complex extract.

<Comparative Example 1> Tanaka extract preparation

100 mL of purified water was added to O.1 g of Tanaka Herbal Powder (NH Market), followed by extraction with a bath at 97°C for 2 hours. After extraction, it was filtered through a 0.45 μm filter to prepare a final Tanaka extract.

<Comparative Example 2> Preparation of Angelicae extract

100 mL of purified water was added to 1 g of Angelica Angelica (Angelica, Jecheon Herbal Herbs), followed by extraction with a bath at 97°C for 2 hours. After extraction, it was filtered through a 0.45㎛ filter to prepare a final Angelicae extract.

<Comparative Example 3> Preparation of windbreak extract

100 mL of purified water was added to 1 g of Bangpung (Bangpung, Jecheon Herbal Herbs), followed by extraction with a bath for 2 hours at 97°C. After extraction, it was filtered through a 0.45㎛ filter to prepare a final windbreak extract.

<Experimental Example 1> Antioxidant test: DPPH scavenging activity

DPPH (2,2-diphenyl-1-picryl hydrezyl) radical scavenging activity was evaluated in order to examine the antioxidant effect using the plant extracts of Example 1 and Comparative Examples 1 to 3.

The plant extracts of Example 1 and Comparative Examples 1 to 3 were mixed with 450 μl of 55 μM DPPH solution by concentration, and then reacted at 4° C. for 30 minutes. After the reaction, each sample was placed in a 96-well plate, and the absorbance was measured at 520 nm using an ELISA reader. At this time, the sample without a sample was used as a control, and the free radical scavenging activity (%) of the sample was calculated as shown in Equation 1 below.

At this time, ascorbic acid was used as a positive control for scavenging radicals, and when the free radical scavenging activity was 50%, the concentration of ascorbic acid was 1.76ug/mL.

[Equation 1]

Free radical scavenging rate (%) = [(1-absorbance of untreated group/absorbance of sample treated group) X 100]

sample IC ₅₀ Example 1 0.33% Comparative Example 1 - Comparative Example 2 0.97% Comparative Example 3 3.18% Ascorbic acid 1.76ug/mL

IC ₅₀ means the sample concentration when the free radical scavenging ability is 50% by the added sample. As a result, as can be seen in Table 1 and Fig. 1, it was confirmed that Example 1 has more excellent antioxidant effects than Comparative Examples 1 to 3.

<Experimental Example 2> Cytotoxicity test: WST (Water-soluble tetrazolium salt) assay

Cytotoxicity experiments were performed using keratinocytes (HaCaT cells) in order to confirm whether the plant extracts of Example 1 and Comparative Examples 1 to 3 have toxicity to cells.

In order to confirm whether the human keratinocyte line is toxic to HaCaT cells, cells were dispensed at a concentration of 1×10 ⁴ cells/well in a 96-well plate using DMEM (Dulbecco's Modified Eagle Medium) medium, and then 37°C and 5% CO ₂ Incubated for 24 hours under conditions. The plant extracts of Example 1 and Comparative Examples 1 to 3 were mixed with a medium by concentration in cells cultured for 24 hours, treated in each well, and cultured for 24 hours at 37°C and 5% CO ₂ conditions, and then the culture solution was removed. Then, each well was treated with a WST-1 assay solution (ez-cytox) and reacted for 2 hours in an incubator, and then absorbance was measured at 450 nm with an ELISA reader, and cell viability was calculated as shown in Equation 2 below.

[Equation 2]

Cell viability (%) = [(absorbance of sample-treated group/absorbance of untreated group) X 100]

As a result, as shown in Figs. 2 to 3, it was confirmed that Example 1 did not show cytotoxicity, so that it could be used as a safe material for cosmetics.

<Experimental Example 3> Skin protection test against ultraviolet light: Cell protective activity against ultraviolet A

Using the plant extracts of Example 1 and Comparative Examples 1 to 3, it was confirmed whether there is an effect of protecting cells from ultraviolet A.

HaCaT cells were treated with the plant extracts of Example 1 and Comparative Examples 1 to 3 for each concentration, and then the cells were irradiated with UV-A of 5J/cm 2 using a UV-A (365 nm) lamp, and then cell viability was measured. . At this time, as a control group, a group not treated with a sample was used.

As a result, as shown in Figure 4, in the case of the control group, when irradiated with ultraviolet A, it was found that the cell viability decreased. On the other hand, in the case of the group treated with the plant complex extract of Example 1, there is an activity to protect cells by inhibiting apoptosis from ultraviolet rays, and compared to Comparative Examples 1 to 3, the cell protective activity against ultraviolet rays of Example 1 is better. Confirmed.

<Production Example 1> Preparation of lotion

A lotion was prepared by a conventional method using the ingredients shown in Table 2 below.

ingredient Content (% by weight) Plant complex extract of Example 1 3.00 Hydroxyethylene cellulose
(2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 Purified water Balance system 100.0

<Production Example 2> lotion production

A lotion was prepared by a conventional method using the ingredients shown in Table 3 below.

ingredient Content (% by weight) Plant complex extract of Example 1 3.00 Ascorbic acid-2-magnesium phosphate salt 1.00 Water-soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.01 Citric acid 0.05 1,3-butylene glycol 3.00 Purified water Balance system 100.0

<Production Example 3> Cream preparation

A lotion was prepared by a conventional method using the ingredients shown in Table 4 below.

ingredient Content (% by weight) Plant complex extract of Example 1 1.00 Polyethylene glycol monostearate 2.00 Self-emulsifying glycerin monostearate 5.00 Cetyl alcohol 4.00 Squalene 6.00 Tri2-ethylhexane glyceryl 6.00 Sphingoglycolipid 1.00 1,3-butylene glycol 7.00 Purified water Balance system 100.0

As described above, specific parts of the present invention have been described in detail, and it will be apparent to those of ordinary skill in the art that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (8)

Antioxidant containing plant complex extracts mixed in a weight ratio of 1:50~500:50~500 with dry weight of Tanaka ( Limonia acidissima Linn.), Angelica gigas Nakai and Windbreak ( Saposhnikovia divaricata Schiskin) as active ingredients And a cosmetic composition for skin protection against ultraviolet rays.
The cosmetic composition for skin protection against antioxidants and ultraviolet rays according to claim 1, wherein the plant complex extract is extracted with a solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof.
The cosmetic composition for skin protection against antioxidants and ultraviolet rays according to claim 1, wherein the plant complex extract is extracted with ethanol.


delete The cosmetic composition of claim 1, wherein the plant complex extract contains 0.1 to 50% by weight based on the total weight of the composition.
The cosmetic composition of claim 1, wherein the plant complex extract has DPPH scavenging activity and cell protective activity against ultraviolet rays.
The cosmetic composition for skin protection against antioxidants and ultraviolet rays according to claim 1, wherein the ultraviolet rays are ultraviolet rays A.
A cosmetic product comprising the composition of any one of claims 1, 2, 3, 5, 6 and 7.

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