KR100789632B1 - Cosmetic for skin whitening containing a herb extract with inhibitory activity of melanin formation - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic containing a plant extract having a melanin production inhibitory activity, the skin whitening cosmetics according to the present invention is selected from the group consisting of fragrance extract, top skin extract, balloon orchid extract and mixtures thereof It is characterized by containing any one as an active ingredient. Since the extract of hyangpo, topi extract and balloon orchid extract is a natural substance, it can be used safely without side effects on the skin, and because it inhibits melanin production and has excellent pigmentation inhibitory effect, cosmetics containing them as active ingredients are blemishes and freckles. And very effective for skin whitening.

Incense * Sea skin * Balloon orchid * Extract * Whitening * Cosmetics

Description

Cosmetic for skin whitening containing a plant extract having a melanogenesis inhibitory activity {Cosmetic for skin whitening containing a herb extract with inhibitory activity of melanin formation}

The present invention relates to a skin whitening cosmetic, and more particularly to a skin whitening cosmetic containing a plant extract having a melanin production inhibitory activity can be used safely without side effects on the skin.

It is everyone's constant desire to have white, fair skin. Human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, it is also influenced by environmental or physiological conditions such as ultraviolet rays, fatigue and stress. Melanin is a type of amino acid tyrosine (tyrosinase) acts as an enzyme called tyrosinase (tyrosinase) is converted to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway by which melanin is made is known, it is still not clear what mechanisms induce melanin synthesis, the previous step in which tyrosinase works.

When such synthesis of melanin occurs excessively in the skin, it may darken the skin tone, and may cause spots and freckles. Therefore, by inhibiting the synthesis of melanin pigment in the skin, not only can brighten the skin tone to realize skin whitening, but also improve skin hyperpigmentation such as spots, freckles, etc. caused by ultraviolet rays, hormones and genetic causes. have.

Therefore, conventionally, a substance having an inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, kojic acid, glutathione, and the like, is formulated into a cosmetic such as an ointment or an essence. Whitening was realized or skin hyperpigmentation such as blemishes and freckles was improved. However, hydroquinone exhibits a predetermined whitening effect, but has a problem of limiting the blending amount to a very small amount due to severe skin irritation. Kojic acid has the disadvantage of being unstable in solution, which complicates the manufacturing process of the cosmetic. In addition, thiol-based compounds such as glutathione and cysteine not only have a characteristic unpleasant odor, but also have problems with transdermal absorption, and glycosides and derivatives thereof have high polarity, making it difficult to use as a cosmetic ingredient. Placenta extract, on the other hand, has no irritation to the skin, but lacks a whitening effect.

Therefore, the technical problem to be achieved by the present invention is to solve the above problems can be used safely without side effects on the skin, and to provide a cosmetic for skin whitening excellent in inhibiting the pigmentation effect by inhibiting the production of melanin.

In order to achieve the above technical problem, the present invention provides a cosmetic for skin whitening containing any one selected from the group consisting of fragrance extract, toffee extract, balloon orchid extract and mixtures thereof.

It is preferable that any one dry weight selected from the group consisting of the sachet extract, topipi extract, balloon orchid extract and mixtures thereof is 0.0001 to 10.0% by weight based on the total weight of the cosmetic.

In addition, the fragrance extract, topi extract and balloon orchid extract is preferably extracted with one or more solvents selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, chloroform and acetone, heat extraction with the solvent Then, it is more preferable that the extract obtained by removing the insoluble substance is distilled under reduced pressure and lyophilized or spray dried.

Hereinafter, the cosmetic for skin whitening according to the present invention will be described in detail.

The cosmetic for skin whitening according to the present invention includes a natural substance having melanin production inhibitory activity, a sachet extract, a top skin extract, a balloon orchid extract or a mixture thereof as an active ingredient.

Perfume ( Typha angustifolia L.) is a perennial herbaceous plant belonging to the vulgaris.

In addition, Pseudolarx kaempferi Gord. Is a bark or root bark of pine family gold transmission, contains tannins and mucopolysaccharides, and is known to be effective in treating topical neurodermatitis (TCM).

In addition, balloon orchid ( Calypso bulbosa Reichb, fil. ) Is a perennial plant belonging to the orchid family.

As such, sachets, toffees, and balloon orchids are known as herbal medicines that can cure urinary bleeding, dermatitis, etc., but the present inventors can screen for substances that inhibit melanin synthesis induction by using their extracts. Experiments with mouse melanoma cells (B16 mouse melanoma cells) revealed that they exhibit very potent melanin suppression and whitening effects. Therefore, when the extract of natural fragrances, toffees and balloon orchids are added to cosmetics such as skin ointments, essences, creams, etc., the skin whitening effect can be exhibited without any side effects.

Sachets, stalks and balloon orchids are readily available on the market, and their extracts include, for example, scents, stalks and balloon orchids in water or anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, ethyl acetate, chloroform, acetone. After heating and extracting with an organic solvent, such as concentrated under reduced pressure it can be obtained by freeze drying or spray drying. At the time of extraction, it is preferable to finely crush sachets, ground skins or balloon orchids, and to extract water or an organic solvent by heating it at 50 to 100 ° C. for 1 to 5 hours with a volume of 5 to 20 to the dry weight of the ground product. . To obtain a high purity extract, the extract may be extracted one or more times in the same manner.

The sachets, toffees or balloon orchid extracts obtained as described above may be used, respectively or simultaneously, in various cosmetics such as skin external ointments or lotions. The content of fragrance extract, toffee extract, balloon orchid extract or a mixture thereof contained in the preparation of the cosmetic according to the present invention is preferably a dry weight of 0.0001 to 10.0% by weight based on the total weight of the cosmetic, more preferably 0.0005 to 10.0% by weight. If the content is less than 0.0001% by weight, it is difficult to expect a clear melanin production inhibitory effect and a whitening effect, and when it exceeds 10.0% by weight, no clear effect is expected as the increasing content.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example of extracting sachets, ground skin and balloon orchid

Extraction Example 1

Purchasing a commercially available sachet was added 100g of the ground pulverized product to 3L of 80% methanol, and extracted by boiling for 3 hours in a reflux extractor with a cooling capacitor. Subsequently, the resultant was filtered through a 100 mesh filter cloth, and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble matters, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to obtain 14 g of dry extract.                     

Extraction Example 2-8

Except for changing the solvent according to the description in Table 1 instead of 80% methanol was carried out in the same manner as in Extraction Example 1.

Extraction Example 9

The same procedure as in Extraction Example 1 was carried out except that the top cover was used instead of the sachet.

Extraction Example 10-16

Except for changing the solvent according to the description in Table 1 instead of 80% methanol was carried out in the same manner as in Extraction Example 7.

Extraction Example 17

Except for using the balloon orchid instead of a sachet was carried out in the same manner as in Extraction Example 1.

Extraction Example 18 ~ 24

Except for changing the solvent according to the description in Table 1 instead of 80% methanol was carried out in the same manner as in Extraction Example 7.

sample menstruum Dry extract (g) Extraction Example 1 Incense 80% methanol 6.5 Extraction Example 2 Incense Purified water 12 Extraction Example 3 Incense 85% ethanol 13.5 Extraction Example 4 Incense 70% acetone 9.6 Extraction Example 5 Incense 50% ethanol 10.3 Extraction Example 6 Incense 60% methanol 12 Extraction Example 7 Incense Saturated normal butanol 8.8 Extraction Example 8 Incense chloroform 7.8 Extraction Example 9 Topography 80% methanol 7.3 Extraction Example 10 Topography Purified water 21 Extraction Example 11 Topography 85% ethanol 17 Extraction Example 12 Topography 70% acetone 12 Extraction Example 13 Topography 50% ethanol 14 Extraction Example 14 Topography 60% methanol 20 Extraction Example 15 Topography Saturated normal butanol 9 Extraction Example 16 Topography chloroform 9 Extraction Example 17 Balloon orchid 80% methanol 21 Extraction Example 18 Balloon orchid Purified water 24 Extraction Example 19 Balloon orchid 85% ethanol 23 Extraction Example 20 Balloon orchid 70% acetone 18 Extraction Example 21 Balloon orchid 50% ethanol 19 Extraction Example 22 Balloon orchid 60% methanol 24 Extraction Example 23 Balloon orchid Saturated normal butanol 15 Extraction Example 24 Balloon orchid chloroform 15

Examples 1-18

Each of the extracts obtained in accordance with the extraction examples 1 to 24 and the aqueous hydroquinone solution was added to the culture of mouse melanoma cells (B-16 mouse melanoma cells) to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). Each of the extracts obtained in accordance with the extraction examples 1 to 12 to a final concentration of 0.005% (Examples 1 to 24), the concentration of the hydroquinone aqueous solution was added to the culture medium of B-16 melanoma cells to 0.0001% After incubation for 3 days, the cells were trypsin-treated and removed from the culture vessel and centrifuged to extract melanin.

1 ml of sodium hydroxide solution (1N concentration) was added thereto to boil for 10 minutes to dissolve the melanin, and the absorbance was measured at 475 nm using a spectrophotometer to express the amount of melanin produced per unit cell count (10 ⁶ cell). In addition, the relative melanin production relative to the control was calculated as the inhibition rate (%) and the results are shown in Table 2 below.

sample Melanin production  % Inhibition Control group (no addition) 0.047 ± 0.003 - Example 1 (final concentration 0.005%) 0.024 ± 0.002 49% Example 2 (final concentration 0.005%) 0.028 ± 0.003 40% Example 3 (final concentration 0.005%) 0.024 ± 0.002 49% Example 4 (final concentration 0.005%) 0.028 ± 0.002 40% Example 5 (final concentration 0.005%) 0.023 ± 0.003 51% Example 6 (final concentration 0.005%) 0.024 ± 0.003 49% Example 7 (final concentration 0.005%) 0.023 ± 0.003 51% Example 8 (final concentration 0.005%) 0.022 ± 0.002 53% Example 9 (final concentration 0.005%) 0.023 ± 0.002 51% Example 10 (final concentration 0.005%) 0.026 ± 0.003 45% Example 11 (final concentration 0.005%) 0.024 ± 0.002 49% Example 12 (final concentration 0.005%) 0.028 ± 0.002 40% Example 13 (final concentration 0.005%) 0.022 ± 0.003 53% Example 14 (final concentration 0.005%) 0.023 ± 0.004 51% Example 15 (final concentration 0.005%) 0.022 ± 0.002 53% Example 16 (final concentration 0.005%) 0.022 ± 0.002 53% Example 17 (final concentration 0.005%) 0.024 ± 0.003 49% Example 18 (final concentration 0.005%) 0.028 ± 0.003 40% Example 19 (final concentration 0.005%) 0.022 ± 0.002 53% Example 20 (final concentration 0.005%) 0.027 ± 0.004 43% Example 21 (final concentration 0.005%) 0.024 ± 0.002 49% Example 22 (final concentration 0.005%) 0.023 ± 0.002 51% Example 23 (final concentration 0.005%) 0.023 ± 0.002 51% Example 24 (final concentration 0.005%) 0.023 ± 0.003 51% Hydroquinone (final concentration 0.0001%) 0.025 ± 0.002 47%

* Repeat count = 3

Referring to Table 2, it can be seen that each sample obtained according to the extraction examples 1 to 24 has a very strong melanin production inhibitory ability against the melanoma cells of the cultured mice compared to the control group (Examples 1 to 24). Considering that it is an extract that is not purified at all, it can be seen that the effect does not decrease even when compared with hydroquinone, a previously known whitening substance.

Examples 25-36

The melanoma production inhibitory ability of the rat melanoma cells cultured in the same manner as in Example 1 was investigated according to the concentration change of the extracts of the extraction examples 1, 9 and 17, and the results are shown in Table 3 below.

sample Application concentration (%) Melanin production % Inhibition Control group (no addition) - 0.047 ± 0.004 - Example 25 Extract of Extraction Example 1 Final concentration (0.0005%) 0.041 ± 0.003 13% Example 26 Final concentration (0.005%) 0.024 ± 0.002 49% Example 27 Final concentration (0.05%) 0.021 ± 0.002 55% Example 28 Final concentration (0.5%) 0.017 ± 0.001 64% Example 29 Extract of Extraction Example 9 Final concentration (0.0005%) 0.042 ± 0.002 11% Example 30 Final concentration (0.005%) 0.023 ± 0.002 51% Example 31 Final concentration (0.05%) 0.021 ± 0.002 55% Example 32 Final concentration (0.5%) 0.016 ± 0.001 66% Example 33 Extract of Extraction Example 17 Final concentration (0.0005%) 0.040 ± 0.002 15% Example 34 Final concentration (0.005%) 0.024 ± 0.003 49% Example 35 Final concentration (0.05%) 0.022 ± 0.002 53% Example 36 Final concentration (0.5%) 0.015 ± 0.001 68% Hydroquinone Final concentration (0.0001%) 0.025 ± 0.002 47%

* Repeat count = 3

As shown in the results of Table 3, it can be seen that the higher the concentration of the extract of sachets, toffees and balloon orchids, the melanin production inhibitory ability on the cultured melanoma cells also significantly improved.

On the other hand, hydroquinone has a strong melanin production inhibitory ability, but exhibits severe cytotoxicity even at low concentrations of 0.0001%, the extract of natural plants according to the present invention does not show cytotoxicity even at 0.05% or more concentrations higher melanin production than hydroquinone It can be made to have an inhibitory effect.

In the following, the fragrance, sapphire or balloon orchid extract is added to the skin ointment, cream, supple cosmetics, nourishing cosmetics, packs, essences to prepare the cosmetics, and to examine the pigmentation inhibitory effect by prescribing them to the test subjects .

Preparation Examples 1 to 3

Perfume extract obtained from the extraction example 5, topi extract from the extraction example 13, balloon orchid extract obtained from the extraction example 21 was added as an active ingredient, respectively, to prepare a skin external ointment with the ingredients and contents shown in Table 4.

Comparative Example 1

Without adding a plant extract, the skin external ointment was prepared with the ingredients and contents shown in Table 4 below.

Composition (% by weight) Production Example Comparative example One 2 3 One Plant extracts Sachet extract 10 - - - Geophytic Extract - 10 - - Balloon orchid extract - - 10 - Diethyl sebacate 8 8 8 8 Prepayment 5 5 5 5 Polyoxyethylene oleyl ether phosphate 6 6 6 6 Sodium benzoate Quantity Quantity Quantity Quantity vaseline to 100 to 100 to 100 to 100

Preparation Examples 4 to 6

Perfume extract obtained from the extraction example 5, topi extract from the extraction example 13, balloon orchid extract obtained from the extraction example 21 was added as an active ingredient, and the cream was prepared in the ingredients and contents shown in Table 5 below.

Comparative Example 2

Cream was prepared with the ingredients and contents shown in Table 5 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 4 5 6 2 Plant extracts Sachet extract 1.0 - - - Geophytic Extract - 1.0 - - Balloon orchid extract - - 10 - Stearic acid 15.0 15.0 15.0 15.0 Cetanol 1.0 1.0 1.0 1.0 Potassium hydroxide 0.7 0.7 0.7 0.7 glycerin 5.0 5.0 5.0 5.0 Propylene glycol 3.0 3.0 3.0 3.0 antiseptic Quantity Quantity Quantity Quantity incense Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100

Preparation Examples 7 to 9

Perfume extract obtained from the extraction example 5, topipi extract obtained from the extraction example 13, balloon orchid extract obtained from the extraction example 21 was added as an active ingredient, respectively, and the softening cosmetic water was prepared with the ingredients and contents shown in Table 6.

Comparative Example 3

Without adding a plant extract, softening water was prepared with the ingredients and contents shown in Table 6.

Composition (% by weight) Production Example Comparative example 7 8 9 3 Plant extracts Sachet extract 0.2 - - - Geophytic Extract - 0.2 - - Balloon orchid extract - - 0.2 - ethanol 10.0 10.0 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 0.2 0.2 glycerin 5.0 5.0 5.0 5.0 1,3-butylene glycol 6.0 6.0 6.0 6.0 incense Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100

Preparation Examples 10 to 12

Perfume extract obtained from the extraction example 5, topipi extract obtained from the extraction example 13, balloon orchid extract obtained from the extraction example 21 was added as an active ingredient, and nutrient cosmetic water was prepared by the ingredients and contents shown in Table 7 below.

Comparative Example 4

No nutrient extract was prepared using the ingredients and contents shown in Table 7 below without adding a plant extract.

Composition (% by weight) Production Example Comparative example 10 11 12 4 Plant extracts Sachet extract 0.1 - - - Geophytic Extract - 0.1 - - Balloon orchid extract - - 0.1 - Waselin 2.0 2.0 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Quantity Quantity Propylene glycol 5.0 5.0 5.0 5.0 ethanol 3.2 3.2 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 18.0 18.0 Potassium hydroxide 0.1 0.1 0.1 0.1 Pigment Quantity Quantity Quantity Quantity incense Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100

Preparation Examples 13 to 15

Perfume extract obtained from Extraction Example 5, topipi extract obtained from Extraction Example 13, balloon orchid extract obtained from Extraction Example 21 was added as an active ingredient, and a pack was prepared with the ingredients and contents shown in Table 8 below.

Comparative Example 5

Packs were prepared with the ingredients and contents shown in Table 8 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 13 14 15 5 Plant extracts Sachet extract 0.5 - - - Geophytic Extract - 0.5 - - Balloon orchid extract - - 0.5 - glycerin 5.0 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 4.0 Polyvinyl alcohol 15.0 15.0 15.0 15.0 ethanol 8.0 8.0 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 0.2 0.2 incense Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100

Preparation Examples 16-18

Perfume extract obtained from the extraction example 5, topi extract from the extraction example 13, balloon orchid extract obtained from the extraction example 21 was added as an active ingredient, and the essence was prepared in the ingredients and contents shown in Table 9 below.                     

Comparative Example 6

Essence was prepared with the ingredients and contents shown in Table 9 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 16 17 18 6 Plant extracts Sachet extract 5 - - - Geophytic Extract - 5 - - Balloon orchid extract - - 5 - Propylene glycol 10.0 10.0 10.0 10.0 glycerin 10.0 10.0 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 0.1 0.1 incense Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100

The method used to verify the pigmentation inhibitory effect by the skin external ointment, cream, supple cosmetics, nutrient cosmetics, pack, essence prepared as described above is as follows.

First, select 20 healthy men and women and attach aluminum foil with two rows of 6 holes with a diameter of 7 mm in the lower part of both arms. Was investigated. The irradiation site was washed well with 70% ethanol aqueous solution before irradiation. 3 days before irradiation to 3 weeks after irradiation twice a day cosmetics containing the sachets, stalk or balloon orchid extract prepared according to Preparation Examples 1 to 18, and plant extracts according to Comparative Examples 1 to 6 Bases were applied in pairs on the same row. Here, the pack agents of Production Examples 13, 14, 15 and Comparative Example 5 were removed 15 minutes after the application.

After prescribing the cosmetics according to the respective production examples and comparative examples according to the above method, the degree of pigmentation was visually determined, and the effect of the pigmentation inhibition of the cosmetics according to each example and the cosmetics according to the comparative example was compared to the apparent effect. Evaluated in three stages, there is no effect, no difference, and also examined the occurrence of skin side effects are shown in Table 10 below.

Experimental substance Distinct Effects (persons) Effective (persons) No difference (persons)  Side effects (persons) Preparation Example 1 6 8 6 0 Preparation Example 2 5 9 6 0 Preparation Example 3 3 13 4 0 Preparation Example 4 3 12 5 0 Preparation Example 5 2 14 4 0 Preparation Example 6 2 15 3 0 Preparation Example 7 2 9 9 0 Preparation Example 8 4 11 5 0 Preparation Example 9 2 10 8 0 Preparation Example 10 3 8 9 0 Preparation Example 11 4 8 8 0 Preparation Example 12 3 10 7 0 Preparation Example 13 4 8 8 0 Preparation Example 14 3 9 8 0 Preparation Example 15 2 7 11 0 Preparation Example 16 3 6 11 0 Preparation Example 17 4 8 8 0 Preparation Example 18 4 9 7 0

As shown in Table 10, the cosmetics containing the sachets, the top skin or balloon orchid extract prepared according to Preparation Examples 1 to 18 showed a significant skin whitening effect on at least 10 or more of 20 subjects, especially many The cosmetic preparations of Preparation Examples 1, 2 and 3, which contained a sheep scent and a top skin extract, showed a clearer inhibitory effect on pigmentation than the cosmetics of Comparative Example 1, which did not contain plant extracts, and did not show any side effects in the skin. Could know.

As described above, the cosmetics for skin whitening containing the fragrance, toffee or balloon orchid extract of the present invention inhibit melanin production and inhibit pigmentation, which is effective for improving skin whitening or freckles. In addition, because sachets or top skins are natural substances, they can be safely used as cosmetics without side effects on the skin.

Claims (3)

Skin cosmetic whitening cosmetics, characterized in that it contains any one selected from the group consisting of top skin extract, balloon orchid extract and mixtures thereof. The skin whitening cosmetic according to claim 1, wherein the dry weight of any one selected from the group consisting of the toffee extract, the balloon orchid extract, and a mixture thereof is 0.0001 to 10.0 wt% based on the total weight of the cosmetic. The method of claim 1, wherein the extract of the top skin or balloon orchid extract is a skin whitening cosmetic, characterized in that extracted with one or more solvents selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, chloroform and acetone .