KR100466379B1 - Composition for skin whitening - Google Patents

Abstract

The present invention relates to a composition for skin whitening, containing the extract of white bran as an active ingredient and having no side effects on the skin and having excellent skin whitening effect, which can be formulated as an external skin ointment or various cosmetics.

Description

Composition for skin whitening

The present invention relates to a composition for skin whitening, and more particularly, to a composition for skin whitening, which has an effect of inhibiting melanin production, which can be formulated as an ointment or various cosmetics for improving blemishes and freckles and skin whitening, as an active ingredient. .

In general, the desire to have white and fair skin is the constant desire of everyone, but the color of a person's skin is genetically determined by the concentration and distribution of melanin in the skin, and also by environmental or physiological conditions such as sun ultraviolet rays, fatigue and stress. It is affected by the color difference of each individual. Melanin is a type of amino acid tyrosine (tyrosinase) acts as an enzyme called tyrosinase (tyrosinase) is converted to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction.

As such, the pathway by which melanin is produced is known, but the mechanisms that induce melanin synthesis, the previous stages of tyrosinase action, are still unknown.

Therefore, in order to have white and fair skin, substances having inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, high acid, glutathione, and the like, have been used in ointments or cosmetics for the purpose of improving skin whitening, blemishes and freckles.

However, they cause skin irritation or poor product stability, which limits their use, and also has a weak effect.

Accordingly, the present invention is to solve the above-mentioned disadvantages, the object of the present invention is to provide a skin whitening composition that is harmless to the skin and excellent skin whitening effect.

Skin whitening composition of the present invention for achieving the above object is to make the whitening extract as an active ingredient.

The whitening composition in the skin whitening composition is preferably contained in 0.001 ~ 10.0% by weight of dry weight can exhibit a good skin whitening effect.

Hereinafter, the configuration and operation of the present invention will be described in detail.

As described above, the composition for skin whitening of the present invention is to have the whitening extract as an active ingredient, and the present invention is the first to find that the whitening extract has a skin whitening effect and apply it to the composition for skin whitening.

In other words, the present inventors conducted a study to develop a new whitening agent for native plants and animals using B16 mouse melanoma cells that can screen a substance that inhibits melanin synthesis induction itself , It is found that the whitening extract has a very strong melanin inhibitory effect and whitening effect, it was applied to the skin whitening composition.

The white matter is also called jjiljiji, the fruit of serialization herbace, the Latin name is Fructus trubuli. Zhigo, the plant of the origin of white zirconia, belongs to the Zygophyllaceae family, and its scientific name is Trubulus terrestris L. Baekji-ryeo has been traditionally used as a herbal medicine for headaches such as vertigo, moxibustion, skin sophistication, milk slack, and amenorrhea.

However, it is completely distinguished from the traditional efficacy of such lightening and the skin lightening effect of the present invention.

On the other hand, prostate hypertrophy treatment (Japanese Patent JP92-169534), dermatitis treatment (Japanese Patent JP91-190809), antiseptic, anti-inflammatory, antipyretic, analgesic, diuretic (SU78-633528) It is known that it can be used for atherosclerosis treatment (SU77-567449), but it is also completely distinguished from the present invention.

Existing studies of whitening components have reported flavonoid compounds such as diosgenin and tribuloside.

The white chrysanthemum extract of the present invention can be obtained through the following process.

You can purchase white Zhijichi, which is sold as a medicine, and extract it first with an organic solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone. .

When the dried baekchul extract was treated to mouse melanoma cells, it showed a very strong melanin synthesis inhibitory effect.

Various cosmetics such as skin ointment or lotion containing the white extract obtained as described above can be prepared, and when applied to the skin, the skin whitening effect is very excellent.

In addition, when the content of white extract was contained in the concentration of 0.001% by weight or less in the preparation of external skin ointment or cosmetics, a clear skin whitening effect could not be expected, and when contained in the concentration of 10% by weight or more, the skin was whitened with increasing content. Since there was no increase in the effect, it is appropriate to contain the content of the baekchul extract in a dry weight of 0.001 ~ 10.0% by weight, preferably containing 0.01 ~ 5.0% by weight showed an excellent skin whitening effect.

Although the present invention will be described in detail based on the following examples, it should be understood that the present invention is not limited only to the following examples.

Example 1.

300 g of the white crushed powder was added to 3 L of 80% methanol solution, boiled for 3 hours in a reflux extractor equipped with a cooling capacitor, filtered through a 300 mesh filter cloth, and the residue was extracted once more in the same manner. The combined extracts were filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to obtain a dry weight of 17 g.

Example 2.

300 g of crushed granules were placed in 3 L of purified water, and the same procedure as in Example 1 was carried out to obtain a dry weight of 22 g.

Example 3.

300 g of crushed white bread was placed in 3 L of a 85% ethanol solution, and the same procedure as in Example 1 was carried out to obtain a dry weight of 18 g.

Example 4.

300 g of crushed white bread was placed in 3 L of a 70% acetone solution, and the same method as in Example 1 was carried out to obtain a dry weight of 22 g.

Example 5.

300 g of crushed white bread was added to 3 L of a 50% ethanol solution, and the same method as in Example 1 was carried out to obtain a dry weight of 22 g.

Example 6.

300 g of crushed white bread was placed in 3 L of a 60% methanol solution, and the same method as in Example 1 was carried out to obtain 19 g of each dry weight.

Example 7.

300 g of crushed granules were placed in 3 L of saturated normal butanol solution, and the same procedure as in Example 1 was carried out to obtain a dry weight of 17 g.

Example 8.

300 g of a white crushed powder was put in 3 L of chloroform solution, and the same method as in Example 1 was carried out to obtain a dry weight of 15 g.

Experimental Example 1.

Each of the extracts obtained according to Examples 1 to 8 was added to the culture medium of the melanoma cells of mice to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). . Each of the extracts obtained according to Examples 1 to 8 were added to a culture medium of B-16 melanoma cells with hydroquinone aqueous solution, respectively, in a final concentration of 0.1%, followed by incubation for 3 days, and then cells were trypsinized from the culture vessel. The melanin was extracted after centrifugation. To which the added sodium hydroxide solution (1N concentration) 1ml hydroxide dissolved melanin boiled 10 minutes using a spectrophotometer in a manner that indicates the amount of the produced melanin in heupgwangdoeul measured at 400 nanometers to the absorbance of the unit cell allowance (10 ⁶ cell) The melanin production relative to the control group was calculated as inhibition rate (%) and the results are summarized in Table 1 below. Melanin production and inhibition rate are the values of three repeated experiments.

TABLE 1

Inhibitory Effect of Bacillus chinensis Extract on Cell Level

As can be seen from the results of Table 1, considering that each sample obtained according to Examples 1 to 8 is not an extract that is not purified at all, compared to the hydroquinone of the previously known whitening substance hydroquinone for the cultured mouse melanoma cells It can be seen that there is a strong melanin production inhibitory ability.

Experimental Example 2.

Melamine production of rats cultured in the same manner as in Experimental Example 1 was investigated for the melanin production inhibitory ability according to the concentration of the extract of Example 1 and the results are shown in Table 2.

TABLE 2

Inhibitory Effect of Bacillus chinensis Extract on Cell Level

As can be seen from the results in Table 2, the white bran extract has a very strong melanin production inhibitory ability against the cultured melanoma cells. Hydroquinone has strong melanin production inhibitory ability at low concentrations, but the cytotoxicity is so severe that it cannot be tested at more than 0.0001%, whereas the extract of Bacillus vulgaris has higher melanin production inhibitory effect than hydroquinone without showing cytotoxicity at 1% concentration. Has In addition, since white extract has a very high melanin inhibitory ability, considering that it is not a single substance but a mixture, it can be very useful for improving freckles and freckles or skin whitening.

Hereinafter, the preparation example of the cosmetics containing an extract of whitening is shown by the preparation example.

Preparation Example 1 and Comparative Example 1

Skin ointment formulation containing whitening extract was as shown in Table 3. Here, the extract of Echinacea refers to that of Example 7.

TABLE 3

Preparation Example 2 and Comparative Example 2

As a prescription of the cream in the cosmetic composition containing the extract of Baekilgi as shown in Table 4 below. Here, the extract of Echinacea refers to that of Example 7.

TABLE 4

Preparation Example 3 and Comparative Example 3

Formulation of flexible cosmetics in cosmetics containing the extract of Baekilji was as shown in Table 5. Here, the extract of Echinacea refers to that of Example 7.

TABLE 5

Preparation Example 4 and Comparative Example 4

Formulation of nutritional longevity among cosmetics containing the extract of Baekilji was as shown in Table 6. Here, the extract of Echinacea refers to that of Example 7.

TABLE 6

Preparation Example 5 and Comparative Example 5

The formulation of the pack of cosmetics containing the extract of Baekilji was as shown in Table 7. Here, the extract of Echinacea refers to that of Example 7.

TABLE 7

Preparation Example 6 and Comparative Example 6

Formulation of the essence of the cosmetics containing the extract of Baekilgi as shown in Table 8 below. Here, the extract of Echinacea refers to that of Example 7.

TABLE 8

Experimental Example 3.

Twenty healthy men and women were selected to attach aluminum foil with two rows of 6 holes with a diameter of 7 mm in the lower part of both arms and irradiated with 60mJ / cm ² light using ORIEL solar simulator 1000W at a distance of 10cm from the arm. It was. At this time, the irradiation site was well washed with 70% ethanol aqueous solution before irradiation. Twice a day from 3 days before irradiation to 3 weeks after irradiation, the same sample was prepared in pairs of the sample containing the white chlorosis extract prepared according to Preparation Examples 1 to 6 and Comparative Examples 1 to 6 and the base containing no white chlorosis extract. It was applied to a string. The pack formulations of Preparation Example 5 and Comparative Example 5 were removed 15 minutes after application. For each, the pigmentation degree of the preparation example and the comparative example was visually determined, and the degree of suppression of the pigmentation of the preparation example compared to the comparative example was evaluated in three stages of distinct effects, effects, and no differences, and the results are shown in the following table. Same as 9.

TABLE 9

As can be seen from the results of Table 9, the ointment and cosmetics containing the white bran extract prepared according to Preparation Examples 1 to 6 showed a whitening effect on at least 10 or more among 20 subjects, in particular, a large amount of white itch In the comparative experiments of Preparation Example 1 and Comparative Example 1 to which the extract was applied, 35% showed a rich pigmentation inhibitory effect, and no side effects appeared in the skin. It can be seen that it is a skin lightening agent.

As described above, the whitening extract is found to be a stable substance without inhibiting melanin synthesis and skin irritation, and thus can be used as an ointment or cosmetic for skin whitening containing the same.

Claims (2)

Skin whitening composition using the extract as an active ingredient. The composition for skin whitening according to claim 1, wherein the whitening extract is contained in an amount of 0.001 to 10.0% by weight.