KR101589119B1

KR101589119B1 - Cosmetic composition of skin-whitening components for lowering the face -temperature and the method of preparing the same

Info

Publication number: KR101589119B1
Application number: KR1020150107161A
Authority: KR
Inventors: 김인숙
Original assignee: 김인숙
Priority date: 2015-04-09
Filing date: 2015-07-29
Publication date: 2016-01-28

Abstract

The present invention provides a method for producing a cosmetic composition for skin whitening which comprises an antioxidant, a tyrosinase inhibiting substance, and a heat-releasing herbal chelating agent to lower the skin temperature to achieve a skin whitening effect.
To this end, the present invention relates to a medicinal herb extract comprising green tea, a green tea, a lobule, a ginseng, a hwanggi, a ginseng, a gugija, a licorice, a ginseng, and a gold and is characterized by comprising arbutin, marine collagen, silk amino acid, vitamin C, It may further contain natural organic sulfur.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for skin-whitening compositions for lowering the skin temperature,

The present invention relates to a method for preparing a cosmetic composition for skin whitening, and more particularly, to a method for producing a cosmetic composition for skin whitening, which comprises an antioxidant, a tyrosinase inhibiting substance and a heat-releasing herbal cleansing agent, To a method for producing a cosmetic composition for skin whitening.

White, clean skin is the hope of all women. However, there are people who become black and black in the same place at the same time, while others return to clean, white skin after a few days. This difference depends on whether or not the facial skin cells are sensitive to heat and that the skin temperature is rapidly increased.

Melanocytes in the basal layer of the skin secrete melanin to protect the skin from heat when the skin temperature rises above normal due to internal or external factors.

However, in spite of not being excessive heat stimuli, melanocytes frequently secrete pigment to form stain or dullness. In this case, most of the causes are internal bodily factors such as liver, stomach, large intestine, uterine problems and hormonal imbalance. In this case, the face rises with fever, often feels red and feels hot. In addition, the skin becomes dry due to heat and the skin cells become fragile.

Melanin is produced by a nonenzymatic oxidation reaction after tyrosine tyrosine, which is an amino acid, is converted to DOPA or dopaquinone by an enzyme called tyrosinase.

Therefore, a substance having inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, high acid, glutathione and the like, is added to ointments and cosmetics in order to have white and fine skin and has been used for skin whitening, spots and freckles improvement .

However, they are disadvantageous in that they cause irritation to the skin or have poor product stability, which limits the use thereof and also has a small effect.

Therefore, it is inevitable to develop a skin whitening composition containing a herbal medicine component which is not irritating to the skin as a main effective ingredient in consideration of the skin temperature.

Korean Patent No. 10-0466379

SUMMARY OF THE INVENTION The present invention has been conceived to solve the above problems, and an object of the present invention is to provide a skin whitening cosmetic composition comprising an antioxidant, a tyrosinase inhibiting substance and a heat-releasing herbal chelating agent to achieve a skin whitening effect by lowering the facial skin temperature And to provide a method for producing the composition.

In order to attain the above object, the cosmetic composition for skin whitening which gives a whitening effect by lowering the facial skin temperature according to the present invention is a composite herbal medicine composition made of green tea, ginseng, lobster, ginseng, hwanggi, dansam, gugija, licorice, And an extract thereof as an active ingredient.

In one embodiment, the composition of the present invention further comprises a silk amino acid and a marine collagen, which is a collagen component extracted from fish, as an active ingredient.

In another embodiment, the composition of the present invention further comprises at least one of arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur as an active ingredient.

In addition, the compositions according to the present invention are characterized by inhibiting melanin synthesis and tyrosinase activity.

As a preferred embodiment of the present invention, the combined herbal medicine extract may be prepared by mixing the herbal extract (A) obtained by transferring the herb medicine to water and diluting it with water and then distilling and extracting the herbal medicine mixture with 1,3-butylene glycol in an extraction solvent And a solvent extract (B) extracted as a solvent.

It is preferable that the distillation extract (A) and the solvent extract (B) are mixed at a volume ratio of 7: 3.

A method for manufacturing a cosmetic composition for whitening skin, which reduces the facial skin temperature according to the present invention to provide a whitening effect,

(A) preparing a distillation extract (A) by transferring a compound herb medicine comprising green tea, ginseng, lobule, ginseng, hwanggi, ginseng, gugija, licorice, Angelica and golden into water and diluting with water followed by distillation; (B) extracting the herbal medicine with 1,3-butylene glycol as an extraction solvent to prepare a solvent extract (B); After the distillation extract (A) prepared in the step (a) and the solvent extract (B) prepared in the step (b) are mixed, marine collagen which is a collagen component extracted from silk amino acid and fish is added, (C) preparing a whitening liquid (C) by melting the mixture (D) adding a whitening powder (D) made by mixing arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur to the whitening liquid (C) prepared in the step (c) do.

As a preferred embodiment of the production method according to the present invention, the distillation extract (A) and the solvent extract (B) may be mixed in a volume ratio of 7: 3.

According to the present invention, when the composition of the present invention is applied to pigmentation sites such as stamens and the like, skin whitening effect can be remarkably expressed by lowering the skin temperature.

In particular, moisture can be improved by reliably lowering the heat of the skin. By removing active oxygen with an antioxidant, the deposited pigment can be made thinner and the skin can be enriched.

1 is a view for explaining an effective ingredient of a cosmetic composition for skin whitening according to a preferred embodiment of the present invention and a method for producing the same,
FIG. 2 is a photograph showing changes in skin temperature of the skin after use for 1 month in the cosmetic composition for skin whitening according to the present invention. FIG. 2 a shows a photograph before use and FIG. 2 b shows a photograph after use.

The skin whitening cosmetic composition according to the present invention may further contain a silk amino acid, marine collagen, arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur as an active ingredient in the combined herbal extract have.

First, the herbal medicine extract of the present invention will be described in detail.

The compound herb medicine according to the present invention is a mixture of green tea, alfalfa, lobule, ginseng, hwanggi, ginseng, gugija, licorice, Angelica, and golden at a predetermined ratio, It can be: 1: 2: 1: 3: 5: 4: 5: 2: 4: 3.

Hereinafter, the function (action) of each component constituting the herbal medicine is described.

The representative function of green tea is antioxidant, and the main component of antioxidant is catechin. Green tea contains antioxidative effect, which is known as aging and carcinogenic elements, which scavenges active oxygen, has a high content of catechin (40 to 100 times stronger than vitamin C) in the leaves of tea and has a high anticancer activity and antimicrobial action. Which is a function of lipid peroxidation.

Excessive active oxygen in the body causes protein denaturation or DNA breakdown, and skin cells become oxidized, resulting in a dull complexion.

It has a slightly cold and spicy taste, and its efficacy has detoxification and anti-inflammatory action. In particular, a component called quercitrin is excellent for removing the toxins from the skin and expanding capillaries to purify blood.

In addition, it has been experimentally proven that the water of the perennial plant inhibits the swelling and reduces the secretion of the tissue fluid to regenerate the tissue. If fever is frequent on the face and has a chronic mild inflammation, if the skin is exposed to cold, dry weather or intense sunlight, dullness and dullness change more intensively.

The lobules are noted for their warmth, flavor, and poison in the Dongbibo sense. The front and back of the leaf are purple and wrinkled. Lobules are rich in K, Ca and Mg, rich in vitamins A and C and minerals. In particular, the baby leaf has antioxidant activity. Vitamin C and antioxidant activity have the effect of improving skin pigmentation.

The main component of ginseng is saponin. The saponin is composed of 30 kinds of ginsenosides. Ginsenoside is used for preventing adult diseases and aging, detoxifying alcohol and protecting liver, improving blood circulation, antioxidant and anti-inflammatory action, And has improved efficacy over the whole human body.

The function of Hwanggi (action) is as follows.

1) Influence on nonspecific immune function

Hwanggi increases blood leukocyte count and promotes neutrophil and macrophage phagocytosis and killing ability.

2) Influence on specific immunity

Hwanggi promotes antibody production to normal people. The number of antibody-producing cells is increased in the mouse spleen. raises the rejection of anti-host transplantation induced by lymphocytes in malignant tumors in rats. The mechanism is to suppress Ts cells.

3) Anti-aging action

Hwanggi extends the life expectancy of silkworm and Drosophila and promotes the differentiation of cells in human embryonic kidney and young mouse kidney and embryo cell lines and prolongs the survival period of each cell.

In addition, it was found that the extract of Hwanggi promotes the proliferation of dermal dermal cells, has a protective effect against UVB, suppresses NF-κB expression and translocation to the nucleus, and increases procollagen.

The action of Dansam is as follows.

1) Improvement of circulation

The opening of the capillary blood vessels is increased, and the blood flow velocity which is gentle due to the stagnation of blood cells is accelerated.

2) tissue regeneration and regulation of regeneration

Promotes tissue recovery and regeneration. According to the experiment, it is known that the administration of Dansamp relatively quickly removes dead cells, increases phagocytic cells, and differentiates fibroblasts and formation of collagen fibers. This effect is known to be due to the improvement of microcirculation, the change of blood and the dynamical change of local blood flow.

The action of Gugija is as follows.

1) Enhancement and regulation of immune function

Gugija's polysaccharide increases the number of white blood cells and neutrophils.

2) Enhancement of hematopoietic function

Gugija whole bath solution increases the number of white blood cells and restores function.

3) Antitumor effect

Gugija's polysaccharide has a tumor suppressive action. It inhibits DNA synthesis in tumor cells and inhibits cell division, thereby reducing cell proliferation.

4) anti-aging action; Increases the amount of hydroxyproline in skin tissue of mice. It is a kind of crystalline amino acid and a component of collagen.

The action of licorice is as follows.

1) Anti-inflammatory and anti-allergic action

Licorice has anti-inflammatory action similar to corticosterone. Liquorice juice inhibits passive skin hypersensitivity.

2) Antiviral action

It has an action to suppress symptoms such as human immunodeficiency virus, hepatitis virus, varicella-zoster virus and the like. In addition to its direct action on the virus, it induces the production of interferon and enhances the activity of NK cells.

3) detoxification; Licorice has the function of detoxifying drug poisoning, animal poisoning, bacterial toxins, toxic poisoning of toxins and poisoning of living metabolites.

The action of Angelicae is as follows.

1) Hematopoietic and anti-anemic effects

When used alone or in combination with Hwanggi, angiosperms increase the amount of red blood cells and hemoglobin in hemolytic anemia.

2) Nonspecific immunity enhancement

It significantly improves the phagocytic ability of macrophages.

In addition to antimicrobial and antiviral action, gold inhibits histamine secretion, inhibits arachidonic acid metabolism, inhibits acute chronic inflammation, and also acts as anti-inflammatory and anti-inflammatory. In addition, in the study, the antiallergic action of baicalin and baicalein inhibited the secretion of anapalacic transporter by inhibiting the activity of SH-enzyme. A relatively strong anti-inflammatory gold was added to the composition of the present invention because frequent facial skin inflammation eventually results in dull and rough skin texture.

In the present invention, the active ingredient of the herbal medicine is extracted using two methods. In other words, the method of extracting water by transferring into water and the method of extracting into 1,3-butylene glycol are used at the same time. The reason for using the above two methods is that most of the herbal medicines have effective ingredients that are well extracted from water and that they are extracted well from alcohol, so that the effective ingredients of the herbal medicines are extracted as effectively as possible.

The method of transferring the herb medicine to water is as follows.

First, the herbal medicines are added to 15% by weight of water, and the mixture is diluted by pouring twice the water of the herbal liquid which has been squeezed out by using a weak fire and strong fire alternately for about 3 hours. Next, the distilled liquid diluted with water is distilled again, and the distilled liquid is collected to make distillation extract (A).

In the present invention, the main reason for re-distilling the water-diluted solution is to remove the impurities and extract pure pure herbal ingredients, so that the active ingredient can be absorbed to the skin as much as possible. In addition, the distillation not only facilitates the absorption of the skin of the evaporated molecule having a small molecular size, but also makes the active ingredient into a form of Ki to penetrate the skin and reduce irritation.

A method for extracting the herbal medicine into 1,3-butylene glycol is as follows.

The herb medicine is placed in a closed container containing 1,3-butylene glycol at a weight ratio of 3: 7, placed in a cool place (about 15 ° C) for 100 days, and then filtered through a filter paper to prepare a solvent extract (B).

In the present invention, the 1,3-butylene glycol is a viscosity reducing ingredient, and forms a barrier that prevents drying, as with other moisturizing agents. In addition, it prevents moisture in the air of high humidity from flowing into the film formation, and also prevents the crystallization of insoluble matter in cosmetics and also helps dissolve water-insoluble matter.

More specific functions of 1,3-butylene glycol will be described in detail as follows.

First, 1,3-butylene glycol has a very good distribution coefficient, allowing the preservative to work more effectively on the formulation, making the preservative less available.

Second, 1,3-butylene glycol plays a role in preventing the decay of cosmetics by microorganisms, and butylene glycol itself has an antimicrobial effect. For example, it has an inhibitory action against Gram-positive bacteria, negative bacteria, fungi, and yeast.

Third, 1,3-butylene glycol is stable. Generally, there is no harm when applied to skin, and it is not irritating even when applied for a long time.

In summary, the 1,3-butylene glycol itself has a preservative effect and effectively extracts a substance that is not soluble in water, and also acts as a moisturizing agent.

As a most preferred embodiment of the present invention, the herbal medicine extract may be prepared by mixing the distillation extract (A) and the solvent extract (B) in a volume ratio of 7: 3.

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The skin whitening cosmetic composition according to the present invention may further contain, as an active ingredient, silk amino acid and marine collagen, which are collagen components extracted from fish, in the herbal medicine extract.

The silk amino acid forms a peptide in which two or more free amino acids and / or amino acids are bonded by hydrolysis of a cocoon (silk protein). The amino acid and the peptide thus formed are collectively referred to as a silk amino acid, and a pale yellow fine powder to be.

There are eight essential amino acids and ten non essential amino acids: valine, isoleucine, leucine, threonine, lysine, methionine, phenylalanine, and tryptophan. This silk amino acid is very similar to the skin constituent protein of the human body, so the skin is absorbed well, and the skin is excellent in the function of regenerating and nourishing the skin. It also protects the skin from ultraviolet rays and external harmful environments by smoothing the skin and strengthening the skin barrier.

The marine collagen is a collagen extracted from the shell of fish, so it has the effect of animal collagen. Promotes regeneration of the skin and provides moisture and nutrition to the skin. Collagen, elastin, etc. By increasing the amount of elastic elements to protect the skin from external stimuli.

As a preferred embodiment, 5 vol% of the silk amino acid and marine collagen are added to the herbal extract solution (A) and the solvent extract (B) in a volume ratio of 7: 3, (C) can be produced.

The cosmetic composition for skin whitening according to the present invention may further contain at least one of arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur as an active ingredient in the herbal medicine extract.

The arbutin is a natural plant ingredient extracted from a mulberry tree. It is known as a safe substance free from adverse effects on the human body and has a function of inhibiting melanin production by acting directly on tyrosinase.

The vitamin C has excellent antioxidant ability to eliminate harmful oxygen in the body, prevents aging and improves various inflammation symptoms. The antioxidant function makes the stain and stain already formed become soft.

The niacinamide is a water-soluble vitamin B3, also known as nicotinamide, a type of niacin that is a vitamin B group. In addition to ceramide, niacinamide simultaneously promotes the synthesis of cholesterol and fatty acids necessary for the formation of lamellar structure, thereby reducing water loss and improving the lipid content of the epidermis. These niacin amides are essential to our body, and vitamin B3 deficiency can cause serious skin lesions.

In particular, various effects of niacinamide on skin are anti-inflammatory and antioxidant functions, preventing light immunosuppression and photoprotection, and increasing intercellular lipid synthesis. In addition, topical use of niacinamide can effectively reduce pigmentation of facial skin and can lead to improved skin tone. This whitening effect is not due directly to the melanin production process in melanocytes but to the inhibition of melanocyte migration from melanocytes to surrounding keratinocytes.

The above-mentioned vegetable natural organic sulfur (MSM, Methy Sulfonyl Methane) is a non-toxic, odorless sulfur extracted from plants and is a water-soluble white powder of 99.9% of Dimethy Sulfone. Its main ingredient is sulfur amino acid cysteine, which quickly cleanses and detoxifies harmful substances accumulated in the skin.

Because of this efficacy, the ancestors found a sulfur hot spring when they had a skin disease and cooked it. The reason is that the sulfur component improves the keratin function of the skin tissue. In addition, it is an indispensable substance for collagen production because sulfur plays a role in capturing various amino acids involved in the skin collagen branch. The present invention minimizes the production of pigment from melanocytes from ultraviolet rays by increasing the skin volume by adding sulfur.

The function of the natural organic sulfur is described in more detail as follows.

1) Keratin (Keratin) function of skin tissue is further improved to rapidly cleanse and detoxify harmful substances accumulated in the skin.

2) It is sulfur to make several chains of amino acids that make up the collagenase directly involved in the collagen molecule. In other words, the sulfur component acts as an adhesive to aggregate the tissues and tissues of the skin (S-S), so that the skin retains its elasticity by collagen functional action.

3) Used as a treatment for dermatitis (dry skin, wounds, atopic skin, acne skin, etc.). Biochemically, sulfuric acid acts on organic matter to form pentathionic acid. This substance dissolves the keratin of the skin, causing insecticidal and bactericidal action. Therefore, sulfur is a nontoxic sulfur element supplying agent, it removes inflammation and has a strong sterilizing power and plays a good role in dermatitis.

4) Vegetable Natural Organic Sulfur alleviates numerous allergies including food allergies, contact allergies, and air-contact allergies.

As a most preferred embodiment of the present invention, the whitening powder (D) is prepared by mixing the arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur in a weight ratio of 10: 2 to 5: 20: 15 to 18 And the whitening powder (D) is added to the whitening liquid (C), wherein the mixing ratio of the whitening liquid (C) to the whitening powder (D) is 12 to 15: 1 by weight, Can be prepared.

Hereinafter, preferred embodiments of a method for manufacturing a cosmetic composition for skin whitening which gives a whitening effect by lowering the facial skin temperature according to the present invention will be described. (See Fig. 1)

The method of the present invention is a step of extracting a complex herbal medicine extract, which is prepared by transferring a complex herbal medicine comprising green tea, Hwasungcho, lobule, ginseng, Hwanggi, Dansam, Gygi, licorice, (A) distilling the herbal medicine, and (b) extracting the herbal medicine with 1,3-butylene glycol as an extraction solvent.

In step (a), the compound herb medicine mixed at a certain ratio is added to 15% by weight of water, and the mixture is heated for about 3 hours in a Munmu fire or alternatively in a weak fire and a strong fire alternately. After pouring the water in the vessel, it is diluted, and the diluted solution in water is distilled again, and the distillate is collected to make the distillation extract (A).

In one embodiment, the compound herb medicine is selected from the group consisting of green tea of weight ratio 1: 2: 1: 3: 5: 4: 5: 2: 4: 3 Based on the total weight of the composition.

In step (b), the compound herb medicine is placed in a sealed container containing 1,3-butylene glycol, which is a kind of alcohol, at a weight ratio of 3: 7, placed in a cool place (about 15 ° C) for 100 days, And a step of making a solvent extract (B).

In the present invention, the distillation extractant (A) and the solvent extractant (B) are mixed at a predetermined ratio in the step (a) and the step (b) And the solvent extract (B) are mixed at a volume ratio of 7: 3.

In step (c) of the present invention, the distillation extract solution (A) and the solvent extract solution (B) are mixed at a certain ratio to prepare a combined medicinal herb extract solution, and the silk amino acid and marine collagen, which is a collagen component extracted from fish, And then melted by heating to prepare a whitening liquid (C).

In one embodiment, the silk amino acid and the marine collagen are added to the herbal medicine extract solution at 5 vol%, respectively.

In step (d) of the present invention, the whitening powder (D) obtained by mixing the whitening liquid (C) prepared in the step (c) with arbutin, vitamin C, niacinamide, At a predetermined ratio.

In one embodiment, the whitening powder (D) is preferably mixed with the arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur at a weight ratio of 10: 2 to 5: 20: 15 to 18, respectively.

When the whitening powder (D) is added to the whitening liquid (C), the mixing ratio of the whitening liquid (C) to the whitening powder (D) is preferably 12 to 15: 1 by weight.

The cosmetic composition for skin whitening according to the present invention can be used by dissolving the whitening liquid (C) to which the whitening powder (D) has been added, and then applying the composition to a pigment deposit site such as spot or dirt.

FIG. 2 is a photograph showing changes in skin temperature of the skin after use of the cosmetic composition for skin whitening according to Example 3 of the present invention for one month.

More specifically, FIG. 2A is a photograph before use of the skin whitening cosmetic composition of the present invention, wherein a lot of heat is generated on the face, and the face is entirely red.

However, FIG. 2B is a photograph of the face of the same user after using the cosmetic composition for skin whitening of the present invention for one month, showing that the number of red parts decreased considerably have.

As described above, when the cosmetic composition for skin whitening according to the present invention is continuously used on the face, the skin temperature can be continuously lowered to achieve a skin whitening effect.

The cosmetic composition for skin whitening according to the present invention may be used in various formulations such as a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, .

In the following, various examples of the present invention are disclosed and experimental examples and comparative examples in which the effects of the present invention can be explained and prepared are disclosed.

Example One

First, the herbal medicines composed of 10 g of green tea, 20 g of seaweed, 10 g of ginseng, 30 g of ginseng, 50 g of ginseng, 40 g of ginseng, 50 g of gugija, 20 g of licorice, 40 g of ginseng and 30 g of gold were placed in 2000 g of water, , Or diluted by pouring twice the amount of water into the solution of the solution which has been squeezed after alternating between the weak and strong flames, and then distilling it again to collect the distillate to prepare the distillate extract (A).

Then, the same amount of the herbal medicine is placed in a closed container containing 700 g of 1,3-butylene glycol, placed in a cool place (about 15 ° C) for 100 days, and filtered to produce a solvent extract (B).

Then, the distillation extract (A) and the solvent extract (B) are mixed at a volume ratio of 7: 3 to prepare a herbal extract.

Example 2

5 vol% of silk amino acid and marine collagen were added to the herbal medicine extract solution of Example 1, heated and melted, and then cooled to prepare a herbal medicine extract (whitening liquid (C)) to which silk amino acids and marine collagen components were added.

Example 3

The whitening powder (D) consisting of 10 g of arbutin, 15 g of vegetable natural organic sulfur, 20 g of niacinamide and 2 g of vitamin C was added to the herbal medicinal herb extract added with the silk amino acid and marine collagen component of Example 2, (C)] was dissolved in 0.4g of the mixture to dissolve silk amino acid, marine collagen, and whitening powder.

[Experimental Example 1] Antioxidant activity test

The antioxidant activity of the skin whitening cosmetic composition according to the present invention was measured by measuring the free radical scavenging ratio.

The antioxidative effect of the compositions according to Examples 1 to 3 was evaluated by using DPPH (1,1-diphenyl-2-picrylhydrazyl) . First, a standard DPPH 400 mu M solution was prepared, and 200 mu g / ml concentration of each of the composition samples according to Examples 1 to 3 was prepared. Then, the standard DPPH solution was added to each sample in the same ratio After incubation at 37 ° C for 30 minutes, absorbance was measured at 517 nm. Ethanol was used as a control.

The free radical scavenging ratio (F) was determined from the reaction absorbance (Es) for each sample and the reaction absorbance (Er) of the control group using the following equation (1). In addition, free radical scavenging ratios were determined by the same method using butylated hydroxytoluene (BHT: butylated hydroxytoluene) as an antioxidant. The results are shown in Table 1 below.

Equation 1

F (%) = 100 - {(Es / Er) x 100}

Example 1 Example 2 Example 3 BHT Free radical scavenging rate (%) 61.9 62.2 66.9 35.5

As can be seen from the above Table 1, the combined herbal medicine extract according to Example 1 of the present invention showed a much higher free radical scavenging rate than BHT, indicating that the antioxidative effect is excellent. It was also found that as the active ingredients of the present invention are added to the herbal medicinal plant extracts as in Example 2 and Example 3 of the present invention, the antioxidative effect is further improved.

[Experimental Example 2] Melanin production inhibition experiment

The degree of inhibition of melanin formation on the B16F1 melanocyte of the skin whitening cosmetic composition according to the present invention was examined. The B16F1 melanocyte used in this experiment is a mouse-derived cell line and is a cell that secretes a melanin pigment called melanin. During the artificial culture of these cells, samples were treated to compare the degree of reduction of melanin pigment.

To describe the specific experimental method, B16F1 melanocytes were dispensed into 6-well plates at a concentration of 2 per well, and after attaching the cells, the samples were treated at a concentration not causing toxicity and cultured for 72 hours. After incubation for 72 hours, the cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation.

Then, the cell pellet was washed once with PBS, and 1 ml of homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin, and the absorbance of melanin was measured at 490 nm using a microplate reader. Melanin formation inhibition rate (I) was calculated according to the following equation (2).

In addition, the inhibition rate of melanin formation was determined by the same method as above using kojic acid as a whitening ingredient. Experimental concentration of each sample according to the above example was set to 200 μg / ml, and the average value of the results of the experiment three times per each sample is shown in Table 2.

Equation 2

I (%) = {(A-B) / A} 100

In Equation 2, A represents the amount of melanin in the well to which no sample is added, and B represents the amount of melanin in the well to which the sample is added.

Example 1 Example 2 Example 3 Kojic acid Melanin inhibition rate (%) 45.2 46.7 51.6 24.5

As shown in Table 2, the herbal medicine extract according to Example 1 of the present invention showed much higher inhibition of melanin formation (inhibition rate) on B16F1 melanocyte than kojic acid, which is a conventional whitening ingredient. Further, it was shown that as the active ingredients according to Example 2 and Example 3 of the present invention were added, the inhibition rate of melanin formation was further increased.

[Experimental Example 3] Tyrosinase activity inhibition experiment

The skin whitening cosmetic composition according to the present invention was tested for inhibition of tyrosinase activity.

Each of the compositions according to Examples 1 to 3 of the present invention was diluted in a buffer solution to a concentration of 50 μg / ml, 0.5 ml of each sample thus prepared was diluted with a tyrosine solution (0.1 mg / ml solution prepared by dissolving tyrosine in 0.05 M sodium phosphate buffer (pH 6.8)), and the mixture was allowed to stand in a thermostat at 37 ° C. for 10 minutes. Then, 0.5 ml of 200 U / ml tyrosinase was added The reaction was carried out in a thermostat at 37 ° C for 10 minutes.

The tube was then quenched to stop the reaction and the absorbance at 475 nm was measured with a spectrophotometer. As a control group, a buffer solution was used instead of the extracts of Examples 1 to 3. The activity inhibition rate (T) of tyrosinase was calculated according to the following equation (3) using the difference in absorbance, and the results are shown in Table 3.

Equation 3

T (%) = 100- [absorbance of sample / absorbance of control x 100]

Example 1 Example 2 Example 3 Inhibition rate of tyrosinase activity (%) 71 72 75

As can be seen from the above Table 3, all of the cosmetic compositions for skin whitening according to Examples 1 to 3 of the present invention showed that the inhibitory rate of tyrosinase activity was over 70% It was found that the effect of Example 3 in which an effective ingredient for skin whitening was added to the composition was the most excellent.

In order to test the whitening effect and moisturizing effect of the skin whitening cosmetic composition according to the present invention, the following Examples 4 to 6 and Comparative Examples were conducted.

Example 4

The composition of Example 1 was mixed with 10 wt% of glycerin, 5 wt% of glycerin, 8 wt% of liquid paraffin, 2 wt% of polysorbate 60, 1 wt% of sorbitan sesquioleolate, 5 wt% of squalane, 2 wt% 0.5% by weight of amine, 1% by weight of propylene glycol, and 1.5% by weight of a carboxyl vinyl polymer are mixed, and the remaining amount is mixed with purified water to prepare an emulsion.

Example 5

The composition of Example 2 was prepared by mixing 10 wt% of the composition, 5 wt% of glycerin, 8 wt% of liquid paraffin, 2 wt% of polysorbate 60, 1 wt% of sorbitan sesquioleolate, 5 wt% of squalane, 0.5% by weight of amine, 1% by weight of propylene glycol, and 1.5% by weight of a carboxyl vinyl polymer are mixed and the remaining amount is mixed with purified water to prepare an emulsion.

Example 6

The composition of Example 3 was changed to 10 wt%, glycerin 5 wt%, liquid paraffin 8 wt%, polysorbate 60 2 wt%, sorbitan sesquirololate 1 wt%, squalane 5 wt%, wax 2 wt%, triethanol 0.5% by weight of amine, 1% by weight of propylene glycol, and 1.5% by weight of a carboxyl vinyl polymer are mixed, and the remaining amount is mixed with purified water to prepare an emulsion.

Comparative Example

Without mixing any of the compositions of Examples 1 to 3, only 5 wt% of glycerin, 8 wt% of liquid paraffin, 2 wt% of polysorbate 60, 1 wt% of sorbitan sesquioleolate, 5 wt% of squalane %, 2% by weight of beeswax, 0.5% by weight of triethanolamine, 1% by weight of propylene glycol and 1.5% by weight of a carboxyl vinyl polymer, and the remaining amount is mixed with purified water to prepare an emulsion.

Experimental Example 4 Whitening Effect (L * a * b * Measurement of L * Value of Colorimetric System) Experiment

In order to measure the whitening effect on the composition of the present invention, 40 healthy women in the 20th to 40th generations were divided into 4 groups each consisting of 10th to 4th and 6th, After the application, the degree of black and white of the skin was judged using a color difference meter (Minolta CR2002). L * a * b * colorimetric system is used to display color, and L * value (brightness) is used as an index in this experiment. The L * values are from black (L * = 0) to white (L * = 100), and ΔL * after one month is shown in Table 4.

Experimental composition DELTA L * Example 4 10.7 Example 5 11.3 Example 6 12.5 Comparative Example 0.52

As can be seen from the above Table 4, it can be seen that the comparative example in which the herbal medicine composition of the present invention is not contained is significantly lower in the? L * value than in Examples 4 to 6. Thus, it was confirmed that the composition according to the present invention is excellent in skin whitening effect.

[Experimental Example 5] Moisturizing effect experiment

In order to test the moisturizing effect of the composition of the present invention, water was removed from 20 healthy female 20s after cleansing, and after 10 minutes, purified water (control) and the composition according to Examples 4 to 6 were applied on the face, Moisture of the facial skin was measured using [MY-808S] of Scala Co., Ltd., which can be purchased from the market every 10 minutes. To obtain objective results, each subject was measured five times, and the average value of each was shown in Table 5.

The moisture content (%) is a percentage value obtained by subtracting the "pre-application value" from the "post-application water number", and the experimental conditions were set at a windy room temperature of 25 ° C and a relative humidity of 25%.

Time (minutes) Control (%) Example 1 (%) Example 2 (%) Example 3 (%) 10 8.5 60.4 64.8 65.7 20 5.3 58.3 58.6 58.6 30 2.1 54.8 55.1 55.2 40 0.3 47.3 48.0 49.3 50 0.0 39.2 40.3 41.2 60 0.0 31.8 33.7 34.1

As can be seen from Table 5, the composition according to Examples 4 to 6 of the present invention showed that the moisture content of the face skin was 30% or more even after 60 minutes passed. Therefore, it was confirmed that the composition according to the present invention has excellent moisturizing effect.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be apparent to those skilled in the art that various modifications may be made without departing from the technical spirit thereof.

Claims

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A method for producing a cosmetic composition for skin whitening,
The herbal medicines were prepared by mixing the herbal medicines composed of green tea, rhizome, lobule, ginseng, hwanggi, dansam, gugija, licorice, Angelica and golden at a weight ratio of 1: 2: 1: 3: 5: 4: 5: 2: 4: And 15% by weight based on the total weight of the distilled water (A). The mixture was stirred for 3 hours with weak light and strong fire. The water was transferred to water and diluted by pouring twice the water of the extracted solution. (a);
(B) preparing a solvent extract (B) which is extracted by placing the complex herb medicine in a closed container containing 1,3-butylene glycol as an extraction solvent at a weight ratio of 3: 7 at a temperature of 15 ° C for 100 days, )Wow;
The distillation extract (A) prepared in the step (a) and the solvent extract (B) prepared in the step (b) are mixed at a volume ratio of 7: 3 to prepare a combined medicinal herb extract solution, (C) adding silk amino acid and marine collagen, which is a collagen component extracted from fish, to the solution by 5 vol% each, heating and dissolving to prepare a whitening liquid (C);
A whitening powder (D) obtained by mixing arbutin, vitamin C, niacinamide, and vegetable natural organic sulfur at a weight ratio of 10: 2 to 5: 20: 15 to the whitening liquid (C) (D) adding a mixing ratio of the whitening liquid (C) and the whitening powder (D) in a weight ratio of 15: 1;
Wherein the skin surface temperature is lowered to provide a whitening effect.

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