KR100544024B1 - Skin whitening compound - Google Patents

Abstract

The present invention relates to a skin whitening composition containing one or two or more selected from the group consisting of Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract or Samsam extract as a blemish or freckles improvement and skin whitening agent.

Description

Skin whitening compound

The present invention relates to a skin whitening composition containing one or two or more selected from the group consisting of Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, or Samsam extract as a blemish or freckles improvement and skin whitening agent, It relates to a skin whitening composition containing the extract excellent in melanin production inhibitory effect.

The desire to have white and fair skin is the constant desire of everyone, and there are many medicines and cosmetics that respond to this to create white and fair skin.

These medicines and cosmetics play a role in regulating the color of human skin. The color of human skin is genetically determined according to the concentration and distribution of melanin in the skin, but also influenced by environmental or physiological conditions such as sun ultraviolet rays, fatigue and stress. Receive. Melanin is a type of amino acid tyrosine (tyrosinase) acts as an enzyme called tyrosinase (tyrosinase) is converted to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway by which melanin is made is known, it is still unknown in detail the mechanism by which tyrosinase acts, the mechanism that induces melanin synthesis.

Therefore, conventionally, a substance having an inhibitory activity on tyrosinase such as hydroquinone, ascorbic acid, kojic acid, glutathione, and the like is added to the ointment or cosmetics to make skin whitening, blemishes and freckles. Although it has been used for the purpose of improvement, the whitening effect of hydroquinone is recognized, but the amount of use is extremely limited due to severe skin irritation. In addition, ascorbic acid is easily oxidized, and cosmetics containing the same cause problems such as discoloration and deodorization, and thiol compounds such as glutathione and cysteine have a characteristic unpleasant odor and have problems with transdermal absorption.

In addition, these glycosides and derivatives have a high polarity, so there is a problem to use them as a component of a cosmetic. In addition, placenta extract has a problem that the whitening effect is insufficient.

The present invention has been made to solve the above problems, and has an object of providing a skin whitening composition having a high safety while having a strong melanin inhibitory effect and a whitening effect.

The present invention for achieving the above object provides a composition for skin whitening, comprising one or more substances selected from the group consisting of Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract as an active ingredient do.

In the composition for skin whitening, it is preferable to contain at least 0.0001 to 10.0% by weight dry weight of at least one substance selected from the group consisting of Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract in terms of skin whitening effect More preferably 0.0005 to 10.0% by weight. This is because a distinct effect could not be expected at a concentration of less than 0.0001% by weight, and no increase in effect was observed with an increase in content at a concentration of 10% by weight or more.

And, the earl extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract is extracted one or more times with lower alcohol, ethyl acetate or acetone having 1 to 4 carbon atoms, and the extract is freeze-dried or spray-dried after distillation under reduced pressure Can be.

Hereinafter, the configuration and operation of the present invention will be described in detail through examples and experimental examples.

As described above, the present invention provides a composition for skin whitening, characterized in that it contains one or more substances selected from the group consisting of Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Sasam extract, as an active ingredient, The earl is used as a perennial herb as it is or as it is dried after removing the bark. The earl belongs to the family Ranunculaceae, and its scientific name is Paeonia japonica Miyabe et Taketa, which distinguishes it from Paeonia lactiflora Pall. Earl pill has traditionally been used as a herbal medicine as a medicinal herb, analgesic and astringent (biopharmaceutical, Dongmyeongsa, 1988) and is completely distinguished from the skin lightening effect of the present invention. On the other hand, patents for skin whitening agents related to red pepper medicines include Japanese Patent Application Laid-Open No. 7-17846 (used as a whitening agent), and Japanese Patent Application Laid-Open No. 10-17459 (used as a whitening agent for improving skin color nonuniformity). It is distinct from Count. According to the existing research on the identification of the ingredients of Paxol, compounds such as Paeonol, Elemicin, Eugenol, Cresol, and Methyl gallate have been reported.

Lily is a perennial herb that is native to Ryukyu and is cultivated for ornamental purposes. The Latin name is Bulbus Lilii, which grows on wet, fertile lands on hillsides or in forests, and has been planted for 2 years. Lilies belong to the Liliaceae family. Lilium lancifolium is the plant of origin in Korea, and Lilium brownii var. viridulum, Lilium pumilum, Lilium lancifolium in Japan, Lilium brownii var. Colchester, Lilium auratum L., Lilium concolors, Lilium japonicum Thunb., Lilium tenuifolium is the plant of origin. Lily has traditionally been used as a herbal medicine to cure pulmonary tuberculosis, biliary large blood, nervous breakdown, new anxiety (herb), which is distinguished from the skin whitening effect of the present invention. On the other hand, in relation to lilies, there are rough skin improvers (Japanese Patent Application No. 5279241) and pregnant women's cold prevention agents (Japanese Patent Application No. 4169533), but these are completely distinguished from the whitening effect of the present invention and whitening of Lilium speciosum. Although there have been reports of external skin preparations using the effect (Japanese Patent Laid-Open No. 6-128143), this is completely distinct from the lily used in the present invention. Existing studies of lily components have reported compounds such as Regaloside A, F, 1-O-Feruloylglycerol, Sandaracopimaradiene, and 1-O-p-coumaroylglycerol.

Hyunsam is a perennial herb that grows in mountains, and Latin people use the root as Scrophulariae Radix. Hyunsam belongs to the family Scrophulariaceae, and Scrophularia buergeriana in Korea, Scrophularia ningponensis in China, and both species in Japan. Hyunsam has traditionally been used as herbal medicines such as anti-inflammatory, antipyretic, analgesic, throat disease, blood pressure strengthening (new medicine botany, Hakchangsa, 1991), which is distinguished from the skin whitening effect of the present invention. On the other hand, research has been reported as a bone tumor therapeutic agent (CN 1105584) in connection with Hyunsam, but is completely distinguished from the present invention. Existing studies on current ginseng have reported Harpagid, p-methoxyhydroxy cinnamic acid and sugars.

The Yeongneung Hyang (Lysimachia sikokiana Miquel = Lysimachia foenum-graecum Hance) is a perennial herb, Latin is Herba Lysimachiae foenum-Graecumi and grows in a humid place around the valley, in the summer and autumn to use the outpost. Yeongreunghyang belongs to the Primaceae (Primulaceae) and has traditionally been used as herbal medicines such as gamma headache, chest robe only, roundworm disease (herb), which is distinguished from the skin lightening effect of the present invention. Previous studies on the identification of Yeongneung-hyang components have reported compounds such as eugenol and methylchavicol.

The ginseng (Adenophora triphylla var. Japonica and related plants) is a perennial plant that grows frequently in the field and the Latin name uses the root as Radix Adenophorae. The ginseng belongs to the bellflower (Campanulaceae) and the root of ginseng is used as a detoxification and decontamination agent in herbal medicine (Korean Plant Book, Haechangsa, 1993), which is distinguished from the skin lightening effect of the present invention. On the other hand, in relation to samsam, neuropathy treatment (CN 1108114), diabetes treatment (CN 1108108), pyelonephritis treatment (CN 1097994) and the like have been reported, but is completely distinguished from the present invention. Existing studies on the identification of samsam components have reported research on compounds such as Syringinoside, Shashenoside I, II, and Daucosterol.

The present invention is the first extract of Baekjak, lily, Hyunsam, Yeongneunghyang or Sasam which can be purchased as a medicinal herb with an organic solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms or ethyl acetate or acetone, and then concentrated under reduced pressure and then freeze-dried or Spray dried to prepare a primary extract. Baekjak extract, lily extract, Hyunsam extract, Youngneung extract or Samsam extract prepared by this method shows very potent melanin synthesis inhibitory effect in B-16 mouse melanoma cells. In addition, the dried white peony extract, lily extract, Hyunsam extract, Yeongneung hyang extract or Samsam extract is applied to the skin of the human skin ointment or cosmetics, the skin whitening effect is very excellent.

The process of obtaining extracts from Baekjak, lilies, Hyunsam, Yeongneung, and Samsam is as follows. Baekjak, Lily, Hyunsam, Yeongneunghyang, and Samsam, which are commercially available as a herbal medicine, are pulverized and pulverized with 5-20 volumes of water or 1-4 anhydrous or hydrous lower alcohol, ethyl acetate, or acetone. Extract by heating at 50-100 ° C. for 1-5 hours in an extractor with reflux condenser. After filtering with a filter cloth, the residue is extracted one more time in the same manner. The extracts are combined, concentrated under reduced pressure, and then lyophilized or spray dried to obtain a dry extract.

When the dried extract thus obtained was treated to mouse melanoma cells, it showed a very strong melanin synthesis inhibitory effect.

In addition, when the various cosmetics such as skin ointment or lotion containing the dry extract was made and applied to human skin, the skin whitening effect was very excellent.

Example 1

300 g of a commercially available earl crushed powder was put in 3 L of 80% methanol solution, boiled for 3 hours in a reflux extractor equipped with a cooling condenser, filtered through a 300 mesh filter cloth, and the residue was extracted once more in the same manner. . Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, followed by freeze drying to obtain a dry weight of 37 g.

Example 2

300g of the commercially available white currant and pulverized powder were put into 3L of purified water, and it carried out by the same method as Example 1, and obtained dry weight 39g.

Example 3

300 g of a commercially available white currant was placed in 3 L of a 85% ethanol solution, and the same procedure as in Example 1 was carried out to obtain a dry weight of 37 g.

Example 4

300 g of a pulverized product which was purchased commercially and was ground was put in 3 L of a 70% acetone solution, and carried out in the same manner as in Example 1 to obtain a dry weight of 35 g.

Example 5

300 g of a pulverized product obtained by purchasing a commercially available white currant was placed in 3 L of a 50% ethanol solution, and was dried in the same manner as in Example 1 to obtain a dry weight of 38 g.

Example 6

300 g of a commercially available white currant was put in 3 L of 60% methanol solution, and it carried out by the same method as Example 1, and obtained dry weight 39 g.

Example 7

300g of the commercially available white currant and the pulverized powder were put into 3 L of saturated normal butanol solution, and it carried out by the same method as Example 1, and obtained dry weight 36g.

Example 8

300 g of a pulverized product obtained by purchasing a commercially available white currant was put in 3 L of chloroform solution, and was carried out in the same manner as in Example 1 to obtain a dry weight of 35 g.

Example 9

A commercially available lily was pulverized and 300 g of pulverized product was carried out in the same manner as in Example 1 to obtain a dry weight of 43 g.

Example 10

300 g of a pulverized product obtained by purchasing a commercially available lily was pulverized in the same manner as in Example 2 to obtain a dry weight of 45 g.

Example 11

A commercially available lily was pulverized and 300 g of the pulverized product was carried out in the same manner as in Example 3 to obtain a dry weight of 42 g.

Example 12

300 g of a pulverized product obtained by purchasing a commercially available lily was pulverized in the same manner as in Example 4 to obtain a dry weight of 44 g.

Example 13

300 g of a pulverized product obtained by purchasing a commercially available lily was pulverized in the same manner as in Example 5 to obtain a dry weight of 41 g.

Example 14

300 g of a pulverized product obtained by purchasing a commercially available lily was pulverized in the same manner as in Example 6 to obtain a dry weight of 39 g.

Example 15

A commercially available lily was pulverized and 300 g of a pulverized product was carried out in the same manner as in Example 7 to obtain a dry weight of 40 g.

Example 16

A commercially available lily was pulverized and 300 g of a pulverized product was carried out in the same manner as in Example 8 to obtain a dry weight of 41 g.

Example 17

300 g of a pulverized product was purchased in the same manner as in Example 1 to obtain a dry weight of 48 g.

Example 18

300 g of a pulverized product obtained by purchasing and grinding commercial ginseng was carried out in the same manner as in Example 2 to obtain a dry weight of 51 g.

Example 19

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 3 to obtain a dry weight of 46 g.

Example 20

300 g of a pulverized product obtained by purchasing and grinding commercial ginseng was carried out in the same manner as in Example 4 to obtain a dry weight of 48 g.

Example 21

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 5 to obtain a dry weight of 47 g.

Example 22

300 g of a pulverized product obtained by purchasing and grinding commercial ginseng was carried out in the same manner as in Example 6 to obtain a dry weight of 46 g.

Example 23

300 g of a pulverized product was purchased in the same manner as in Example 7 to obtain a dry weight of 42 g.

Example 24

300 g of a pulverized product obtained by purchasing and grinding commercial ginseng was carried out in the same manner as in Example 8 to obtain a dry weight of 44 g.

Example 25

300 g of a pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 1 to obtain a dry weight of 28 g.

Example 26

300 g of a pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 2 to obtain a dry weight of 32 g.

Example 27

300 g of a pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 3 to obtain a dry weight of 31 g.

Example 28

300 g of the pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 4 to obtain a dry weight of 31 g.

Example 29

300 g of a pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 5 to obtain a dry weight of 29 g.

Example 30

300 g of a pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 6 to obtain a dry weight of 30 g.

Example 31

300 g of a pulverized product was purchased in the same manner as in Example 7 to obtain a dry weight of 32 g.

Example 32

300 g of a pulverized product obtained by purchasing a commercially available Yeongneung flavor was pulverized in the same manner as in Example 8 to obtain a dry weight of 30 g.

Example 33

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 1 to obtain a dry weight of 37 g.

Example 34

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 2 to obtain a dry weight of 40 g.

Example 35

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 3 to obtain a dry weight of 37 g.

Example 36

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 4 to obtain a dry weight of 34 g.

Example 37

300 g of a pulverized product obtained by purchasing commercially available ginseng was ground in the same manner as in Example 5 to obtain a dry weight of 35 g.

Example 38

Commercially available ginseng 300g was pulverized and pulverized in the same manner as in Example 6 to obtain a dry weight of 37g.

Example 39

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 7 to obtain a dry weight of 34 g.

Example 40

300 g of a pulverized product obtained by purchasing commercially available ginseng was pulverized in the same manner as in Example 8 to obtain a dry weight of 36 g.

Experimental Example 1

Each extract obtained according to Singye Examples 1-40 was added to the culture of mouse melanoma cells to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). . Each extract obtained according to Example 1-40 was brought to a final concentration of 0.01% and added to the culture medium of B-16 melanoma cells with an aqueous hydroquinone solution, and then cultured for 3 days, followed by trypsin treatment. Melanin was extracted from the culture vessel by centrifugation. To this, 1 ml of sodium hydroxide solution (1N concentration) was added, boiled for 10 minutes to dissolve the melanin, and the absorbance was measured at 400 nm by using a spectrophotometer, indicating the amount of melanin produced as the absorbance of a unit cell count (10 ⁶ cells). The melanin production relative to the control group was calculated as the inhibition rate (%) and the results are summarized in Table 1 below.

As can be seen from the results in Table 1, considering that each sample obtained according to Examples 1-40 is an extract that is not purified at all, it is very potent against the cultured rat melanoma cells compared to hydroquinone, a known whitening substance. It can be seen that the melanin production inhibitory ability.

Experimental Example 2

Melamine production of rats cultured in the same manner as in Experimental Example 1, the extracts of Examples 1, 9, 17, 25, 33 were investigated for the ability to inhibit melanin production according to the concentration.

As can be seen from the results of Table 2, it can be seen that Baekjak extract, lily extract, Hyunsam extract, Youngneung extract, Samsam extract has very strong melanin production inhibitory ability against cultured melanoma cells. Hydroquinone has a strong melanin production inhibitory ability at low concentrations, but the cytotoxicity is severe and can not be experimented at 0.0001% or more, whereas the extracts in the present invention can be used without hydrolysis at 0.01% or more. It has a higher melanin production inhibitory effect than quinone. In addition, the earl extract, lily extract, Hyunsam extract, Yeongneunghyang extract, Samsam extract has very high melanin production inhibitory ability, considering that it is not a single substance, it can be very useful for improving blemishes and freckles or skin whitening. .

Hereinafter, the preparation examples of cosmetics containing Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract is shown by the preparation example.

Preparation Example 1-5 and Comparative Example 1

The prescription examples of the skin ointment containing Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract is as shown in Table 3 below.

Here, Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract was used in Example 5, 13, 21, 29, 37, respectively.

Preparation Example 6-10 and Comparative Example 2

Prescription example of the cream of the cosmetic composition containing Baekjak extract, lily extract, Hyunsam extract, Youngneung extract, Sasam extract is as shown in Table 4 below.

Here, Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract was used in Example 5, 13, 21, 29, 37, respectively.

Preparation Example 11-15 and Comparative Example 3

Prescription examples of softening longevity in cosmetics containing Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract is as shown in Table 5.

Here, Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract was used in Example 5, 13, 21, 29, 37, respectively.

Preparation Example 16-20 and Comparative Example 4

Prescription examples of nutritional lotion in cosmetics containing Baekjak extract, lily extract, Hyunsam extract, Youngneung extract, Samsam extract is as shown in Table 6.

Here, Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract was used in Example 5, 13, 21, 29, 37, respectively.

Preparation Example 21-25 and Comparative Example 5

Prescription examples of the pack of cosmetics containing Baekjak extract, lily extract, Hyunsam extract, Youngneung extract, Sasam extract is as shown in Table 7.

Here, Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract was used in Example 5, 13, 21, 29, 37, respectively.

Preparation Example 26-30 and Comparative Example 6

Prescription example of the essence of cosmetics containing Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract is as shown in Table 8.

Here, Baekjak extract, lily extract, Hyunsam extract, Yeongneung hyang extract, Samsam extract was used in Example 5, 13, 21, 29, 37, respectively.

Experimental Example 3

Twenty healthy men and women were selected to attach aluminum foil in two rows of six holes with a diameter of 7 mm in the lower part of both arms. The irradiation site was washed well with 70% ethanol aqueous solution before irradiation. Twice a day from 3 days before irradiation to 3 weeks after irradiation, each containing a sample containing Baekjak extract, Lily extract, Hyunsam extract, Yeongneunghyang extract, Samsam extract prepared according to Preparation Examples 1-30 and Comparative Examples 1-6, respectively. A base containing no extract of was applied in the same row in pairs. The pack formulations of Preparation Examples 21, 22, 23, 24, 25 and Comparative Example 5 were removed 15 minutes after the application. For each, the pigmentation degree of the preparation example and the comparative example was visually determined, and the degree of suppression of the pigmentation of the preparation example compared to the comparative example was evaluated in three stages of distinct effects, effects, and no differences, and the results are shown in the following table. Same as 9.

As can be seen from the results of Table 9, the whitening ointment or cosmetics containing Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Samsam extract prepared according to Preparation Example 1-30 at least 10 or more among 20 subjects In the comparative experiments of Preparation Examples 1, 2, 3, 4, 5 and Comparative Example 1, in which a large amount of Baekjak extract, Lily extract, Hyunsam extract, Sasam extract were applied, more than 65% of the pigmentation was shown. It shows an inhibitory effect, does not show any side effects in the skin, it can be seen that earl extract, lily extract, Hyunsam extract, Yeongneung extract, Sasam extract is safe and very effective blemishes or freckles improve or skin whitening agent.

As described above, the skin whitening composition containing Baekjak extract, lily extract, Hyunsam extract, Yeongneung extract, Sasam extract is safe and does not have side effects according to the use, it is excellent in whitening effect.

Claims (3)

A composition for skin whitening, comprising one or more substances selected from the group consisting of lily extract, Hyunsam extract, Yeongneung extract, and Samsam extract as active ingredients. The method of claim 1, wherein the skin whitening composition comprises at least one material selected from the group consisting of lily extract, Hyunsam extract, Yeongneung extract, and Samsam extract in a dry weight of 0.0001 to 10.0% by weight. Skin whitening composition. The method of claim 1, wherein the lily extract, Hyunsam extract, Youngneung extract, or Samsam extract is extracted one or more times with lower alcohol, ethyl acetate or acetone having 1 to 4 carbon atoms, and the extract is distilled under reduced pressure and freeze-dried. The composition for skin whitening, which is prepared by spray drying.