KR19980076793A - Skin moisturizer composition containing extract of herbal medicine - Google Patents

Abstract

The present invention relates to a skin moisturizing composition containing a herbal medicine extract, and more particularly, a solvent in a mixed medicine containing at least two or more selected from the Chinese herbal medicines such as jade porridge, lily, peony, lotus root and sulphur It relates to a skin moisturizer composition containing a water extract extracted using water as.

Description

Skin moisturizer composition containing extract of herbal medicine

The present invention relates to a skin moisturizer composition containing herbal medicine extract, more specifically, a mixed herbal medicine containing at least two or more selected from the Chinese herbal medicines such as jade porridge, lily, peony, lotus root and sulphur, which are mainly used in herbal medicine It relates to a skin moisturizer composition containing a mixed herbal drug extract extracted using water as.

Polygonatum officinale is a cardiovascular, adrenal cortex-like hormone containing steroidal saponins such as convallamarin and convallarin and a number of flamooids. It is known to have an action, thirst quenching action, homeostasis action, skin aging prevention action. Lilium longiflorum contains alkaloids such as colchiceine and many muco-polysaccharides, which are known to be effective in lowering heart heat and calming the mind. Peony (Paeonia lactiflora) contains monoteptene glycosides, such as paeoniflorin, catechin, and tetra-undecagalloylglucoside. Sedation, analgesic, vasodilation, anti-inflammatory, antioxidant, contact hypersensitivity action is known. Neulbo nucifera contains a large amount of starch and sugar called raffinose, and its pharmacological actions include mental stability, strengthening of the spleen and stomach, sleep anxiety, skin whitening, inflammation relief, and skin healing. The effect is known. In addition, Penmannia glutinosa contains a lot of sugar oil, such as stachyose, raffinose, sucrose, sucrose, etc., and the effects of hypoglycemia and diuretic relaxation are known. Among them, stachyose is known as an oligosaccharide, forming a protective film on the skin, enhancing water absorption and preventing water evaporation.

Since ancient times, the concept of voicing has been widely used, and when interpreted from the modern perspective, the concept of skin is deeply related to the skin moisturizing effect, and from this perspective, skin moisturizing among ingredients known to be effective The present invention has been researched and developed for the purpose of finding an effective raw material, and in order to solve the stability and preservation problems of the raw material in order to be able to be used as a basic raw material of cosmetics as well as skin moisturizing effect, as a result of completing the present invention Reached.

Hereinafter, the operation and configuration of the present invention will be described.

The skin moisturizer composition according to the present invention is a mixed herbal medicine containing at least two or more selected from jade porridge, lily, peony, lotus root and turmeric extract using water as an extraction solvent, and the mixed herbal medicine water extract liquid thus extracted In 100 parts by weight of the skin moisturizer composition, 1-10 parts by weight of glycerin and 15-25 parts by weight of 1,3-butylene glycol are contained.

The mixed herbal water extract is preferably extracted by mixing the mixed herbal medicine and the extraction solvent water in a ratio of 1: 5 to 1:10 by weight. Most preferably, the mixing ratio of the mixed herbal medicines and water is 1: 5.

After selecting 20 kinds of raw materials (see Table 1), which are known to have the effect of Boeum in oriental medicine, and extracting them using water and 70% ethanol as extraction solvent, Trans Eepidermal WSater Loss; TEWL) to check the skin's moisturizing effect, mixed extraction with the primary searched raw materials to check the total amount of reducing sugar in the extracted extract and the moisturizing effect in the same way as the first search Determine the best mixed extract.

The problem when applying the plant mixture extract to products such as cosmetics is the self-preservation ability for long-term storage as a raw material and stabilization after formulation.

Therefore, in the present invention, the mixed solvent composition was determined based on the solvents commonly used in cosmetics, and the mixed extract solution was dissolved in each mixed solvent to test the antibacterial activity and the degree of stabilization according to the temperature. It was used.

As a result of the experiment, the moisturizing effect of the water extract of the herbal medicine mixtures such as jade porridge, lily, peony, lotus root, and turmeric is very excellent, and as the composition of the mixed solvent, the skin moisturizing composition 100 according to the present invention contains the mixed herbal medicine extract and the mixed solvent. It is preferable that 1-10 weight part of glycerin and 15-25 weight part of 1, 3- butylene glycol are contained in a weight part, and the mixed solvent which contains 5 weight part of glycerin and 15 weight part of 1, 3- butylene glycol is the most preferable. In this mixed solvent composition, the water extract of the mixed herbal medicine is most stably maintained for a long time.

Generally, plant extracts used as cosmetics use solvents such as alcohols as extraction solvents, but in the present invention, following the extraction method applied in Chinese medicine, the extract is extracted while heating and refluxing the water as an extraction solvent. Compared to alcohol extracts, the components of vegetable sugars having high solubility in water were extracted as much as possible.

In the case of water extraction, since the content of polysaccharides is higher than that of solvent extraction and water has little preservative effect, an additional preservative is added because the generation of microorganisms cannot be avoided when the extract is stored as a raw material for a long time. The use of preservatives should be minimized as they may harm the human body or cause skin irritation. Therefore, as a result of several experiments to find a solvent condition that can minimize the loss of vegetable polysaccharides while having an antiseptic effect, 1-10 parts by weight of glycerin and 15-25 weight of 1,3-butylene glycol in the moisturizing composition according to the present invention. It can be seen that both the preservative and the stability of the raw materials are excellent in the addition-containing mixed solvent composition.

Hereinafter, the present invention will be described in detail by production examples and examples.

Preparation Example 1

10 g of each of the natural products shown in Table 1 was added thereto, followed by addition of 100 ml of water, followed by extraction with boiling under reflux for 3 hours, followed by filtration with a filter paper to obtain the filtrate, and adjusted to 100 ml in total by adding water. The mixture was left at 4 ° C. for 6 days to precipitate lowly soluble substances in the extract components, and then filtered once using a separate filtration layer to catch fine substances that were easily precipitated, followed by 0.45 μm membrane filtration. It was.

Preparation Example 2

10 g of each natural product shown in Table 1 was added thereto, and 100 ml of 70% ethanol was added thereto, followed by extraction with boiling under reflux for 3 hours. The filtrate was filtered through a filter paper, and adjusted to a total of 100 ml by adding 70% ethanol. The mixture was left at 4 ° C. for 6 days and then filtered once using a separate filter layer, followed by 0.45 μm membrane filtration.

Example 1 TEWL Measurement

TEWL was measured by a moisture evaporation meter (ServoMED Evaporimeter) in order to first search for a moisturizing substance in extracts prepared in Preparation Examples 1 and 2. Twenty normal guinea pigs were selected to epilate the same area on the back, and several random spots were designated and treated with sodium lauryl sulfate (hereinafter referred to as SLS) to injure skin tissue. Thereafter, the prepared extract was applied to the wound area, and then the value was set to an initial value by reading a value with a moisture evaporation meter. Since the same extract was applied to the wound for 4 days, the value was read using a moisture evaporator on the fifth day. The results are shown in Table 1. In the case of alcohol extracts, the overall moisturizing effect was low and the water extractant had a relatively high moisturizing effect, which is considered to be due to the relatively high ability to contain water because the vegetable saccharides extracted mainly during water extraction are polymers. .

Through the above experiment, in particular, a relatively high degree of moisturizing, jade porridge, lotus root, peony, lily, and sulfur were selected first.

TABLE 1

Moisturizing Effect Test of Extract

Table 1 (continued)

Preparation Example 3

According to the results of Example 1, each of the selected jujuk, lotus root, lily, peony, turmeric each take 10g and mixed by dividing into a single herbal medicine, three mixed herbal medicines, five mixed herbal medicines by the combination as shown in Table 2 below. Next, 100 ml of water was added thereto, followed by extraction, filtration, adjustment, and refiltration in the same manner as in Preparation Example 1, thereby preparing a final extract. The types of extracts prepared are as shown in Table 2.

Example 2 Total Sugar Analysis

10 ml of each extract obtained in Preparation Example 3 was taken, and the content of the reduced total sugar was measured using a known phenol-sulfuric acid method. The results are shown in Table 2 below. As shown in Table 2, the sugar content of the turmeric was the highest in the single herbal extract, and the total sugar content of the five mixed herbal extracts was the highest.

TABLE 2

Total sugar analysis of single and mixed extracts

Example 3 TEWL Measurement

From the results of Example 2, it was confirmed that the sugar content of the mixed herbal medicine extract was higher than the sugar content of the single herbal medicine extract, and the moisturizing effect of the mixed herbal medicine extract was measured. Moisturizing effect was measured in the same manner as in Example 1 and the results are shown in Table 3. As a result, the moisturizing effect of the five mixed herbal extracts, which had the highest sugar content in Example 2, was the best. From the results of Examples 2 and 3, it is assumed that the content of vegetable sugars is an important factor for moisturizing.

TABLE 3

Moisturizing Effect of Mixed Extracts

Example 4 Cell Proliferation Effect Test of Mixed Herbal Medicine Extract

The cell proliferation effect of the mixed herbal medicine extract prepared in Preparation Example 2 was compared. Some keratinocytes were removed from the skin tissue of guinea pigs, and cell proliferation was carried out using a known animal cell culture medium, followed by 96 well plates using a hematocytometer. Each well of the plate) was dispensed with a constant unit (5000 cells) and treated 4 hours after the sample was placed in a concentration gradient of 50%. After incubation for 3-4 days, the cells were treated with dye for dyeing (MTT, 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide), and then spun at high speed with a centrifuge. After sinking to the bottom and the dye crystals were dissolved in dimethyl sulfoxide (dimethyl sulfoxide) was measured by the ELISA reader (ELISA reader) to measure the color difference according to cell proliferation at 540nm, 630nm. The sample untreated group was defined as 100% and the sample treated groups were compared and expressed as a relative percentage (%). The results are shown in Table 4. The cell proliferation effect of the five mixed herbal extracts was much higher than that of the other mixed herbal extracts.

TABLE 4

Cell proliferation effect

Example 5 skin irritation test according to the solvent composition

When preparing a mixed herbal extract extract that is effective for the skin from Examples 1, 2, 3, 4, but is intended to be used as the extract itself in the actual product such as cosmetics, the herbal extract according to the present invention is a water extract using water as a solvent As a result, no preservation of the extract occurs, so storage problems arise as raw materials, and vegetable sugars, which are considered to be a major factor of moisturizing, are often precipitated due to low solubility by other solvents or substances in the formulation of cosmetics. Therefore, in order to prevent self-preservation and to prevent the precipitation of the active ingredient, skin preservation test was performed using preservative, stabilization and solvent addition using basic solvents which are mainly used in the manufacture of cosmetics.

First, three solvents of glycerin, 1, 3-butylene glycol, and propylene glycol were selected to prepare two kinds of mixed solvents in which each of these solvents was divided so that the content in 100 parts by weight of the total composition was 5 parts by weight and 15 parts by weight. 5 kinds of mixed herbal medicine water extract was dissolved. For the skin irritation test, 20 volunteers were selected and the patch test was performed on the arm, and the skin irritation degree according to the mixed solvent composition was classified into five levels (Table 5).

In Table 5, G means glycerin, BG means 1, 3-butylene glycol, and PG means propylene glycol, and the numbers in parentheses mean the concentration of the solvent (content of the solvent contained in 100 parts by weight of the total composition) upon mixing. .

The skin irritation degree is marked as + when the degree of skin irritation is low and +++++ when the irritation degree is high. That is, the greater the number of +, the higher the stimulus, and the lower the number of +, the lower the stimulus.

Analyzing the data from the experimental results, the antimicrobial activity correlated with 1,3-butylene glycol and the skin irritation gradually decreased as the glycerin content increased.

TABLE 5

Skin irritation and antimicrobial activity of mixed solvents

Example 6: Antimicrobial Activity Measurement

For preservation as a raw material, the preservation force by the addition of a preservative mixed solvent other than water was tested. Antimicrobial activity was measured by agar dilution method, and each of the mixed solvents shown in Table 5 was dissolved in various concentrations in a heat-sterilized brain heat infusion agar medium (BHI agar). After pouring into the curry and solidified, the test bacteria were plated and incubated anaerobicly at 37 ℃. Test bacteria are Three species of E. coli, Psedomonas aeruginosa, and Staphylococcus aureus were used, and after 48-72 hours of incubation, the growth of the bacteria was observed. The antimicrobial activity of each sample was compared with the minimum concentration (Minimum Inhibitory Concentration (MIC)). The results showed high antimicrobial activity in several experimental groups as shown in Table 5.

Example 7: Stabilization Test of Extract

From the skin stimulation and antimicrobial activity test results of Examples 5 and 6, three types of mixed solvents that were considered most suitable for application of the product were selected, and stability tests were performed according to temperature. Five mixed herbal water extracts were dissolved in three mixed solvents selected from Examples 5 and 6, and each of 10 ml was taken, and then the stability was compared according to temperature. Put 3 kinds of samples in a thermostat at 4 ℃, 25 ℃, 50 ℃, and leave them for 20 days, put each in a conical tube, rotate them at high speed with a centrifuge, collect the precipitated materials, and dry the weight of the precipitate. Was measured. The experimental results are shown in Table 6, and the extract was most stable in the mixed solvent of 5 parts by weight of glycerin and 15 parts by weight of 1, 3-butylene glycol.

TABLE 6

Stabilization Test of Extracts in Mixed Solvents

Examples 1, 2, 3, 4, 5, 6 through the present invention to determine the mixed herbal medicine water extract with excellent skin moisturizing effect from herbal ingredients and the mixed solvent composition which can be used as a raw material of cosmetic by dissolving the extract According to the skin moisturizer composition.

Example 8 Skin Beauty Effect Measurement

In the following formula 1, the mixed herbal medicine water extract according to the present invention according to the present invention was dissolved in the mixed solution according to the present invention (hereinafter referred to as consonant liquid) and 10.0% by weight of the consonant solution in the following prescription 1 with purified water Except that, 30 women of 20-40 years of age with poor skin were selected using the same cosmetic base as prescription 1, and the condensate solution was applied to the left side of the face and the consonant solution was not applied to the right side. After the time, the skin moisture and the amount of TEWL were measured, and the good one was selected to calculate the number of people. In order to determine the accuracy of the experiment, the average value was repeated for 7 days to obtain the results shown in Table 7.

TABLE 7

Skin beauty effect

TABLE 8

Prescription 1: composition of the cream

Table 8 (continued)

In the cream of Formulation 1 used in the present embodiment, the oil phase part was prepared by heating and stirring the raw material 1-8 at 72 ° C., the raw material 10-11 was added to the raw material 9, and the mixture was heated and stirred to prepare the water phase part. The mixture was put in an aqueous phase and heated at 50 ° C., and then cooled to 30 ° C. to prepare it.

As described above, the skin moisturizer composition containing the herbal medicine extract according to the present invention may not only have excellent skin moisturizing effect but also obtain various pharmacological effects according to the herbal medicines used.

Claims (3)

1-10 parts by weight of glycerin in a skin moisturizer composition containing a mixed herbal medicine water extract liquid extracted using water as a solvent as a mixed herbal medicine containing at least two selected from jade, lily, peony, lotus root and turmeric And 1, 3-butylene glycol skin moisturizer composition containing 15-25 parts by weight. The skin moisturizer composition of claim 1, wherein the mixed herbal medicine water extract solution is extracted by mixing the mixed herbal medicine and the water used as the extraction solvent in a ratio of 1: 5 to 1:10 by weight. The skin moisturizer composition according to claim 1, wherein 5 parts by weight of glycerin and 15 parts by weight of 1, 3-butylene glycol are contained.