KR102337422B1 - Cosmetic composition comprising jojoba oil for skin calming care and skin trouble improvement, and preparing method the same - Google Patents

Abstract

Disclosed are: a cosmetic composition for soothing skin and alleviating skin problems that comprises jojoba oil, hyaluronic acid mixture, glycerin, 1,2-hexanediol, butylene glycol, ethylhexylglycerin and disodium EDTA; and a method for preparing the same. The cosmetic composition according to one embodiment of the present invention is effective in soothing skin and alleviating skin problems.

Description

Cosmetic composition comprising jojoba oil for skin calming and trouble improvement, and preparation method thereof, comprising jojoba oil, and preparing method the same

Examples below relate to a cosmetic composition for soothing skin and improving skin troubles containing jojoba oil and a method for preparing the same.

Interest in beauty is growing, and the importance of youthful and lively skin is being emphasized more and more. An effective way to have good skin is to prevent skin aging. The main causes of skin aging are dryness along with damage to the skin caused by UV rays. To prevent the skin from drying out, use a moisturizer such as lotion to prevent moisture from leaking to the outside as much as possible. Also, to regenerate damaged skin from UV rays, skin rejuvenating agents such as ampoules are used.

Among them, jojoba oil is one of the most popular natural oils. Jojoba oil is a non-volatile oil made by extracting or pressing part of the desert jojoba tree. Jojoba oil is similar to human sebum and contains vitamin A and tocopherol, so it has an excellent antioxidant effect on the skin and helps to prevent skin aging. In addition, it can prevent roughening of the skin and protect the skin from harmful oxygen. Therefore, this jojoba oil is used together with moisture and various ingredients that can help skin health.

However, existing products are not effective enough to improve or prevent skin troubles or skin soothing effects.

Korean Patent Application No. 10-2018-0114937 Korean Patent Application No. 10-2016-0038222

Paying attention to the above problems, the inventors of the present invention contain jojoba oil, hyaluronic acid mixture, glycerin, 1,2-hexanediol, butylene glycol, ethylhexyl glycerin and disodium EDTA, thereby soothing the skin. And to provide a cosmetic composition with improved trouble.

Accordingly, one object of the present invention is to improve skin soothing and trouble by containing jojoba oil, hyaluronic acid mixture, glycerin, 1,2-hexanediol, butylene glycol, ethylhexyl glycerin and disodium EDTA. An object of the present invention is to provide a cosmetic composition.

The object of the present invention is not limited to the object mentioned above. The objects of the present invention will become more apparent from the following description, and will be realized by means and combinations thereof described in the claims.

The cosmetic composition for soothing skin and improving troubles according to an embodiment includes jojoba oil, a mixture of hyaluronic acid, glycerin, 1,2-hexanediol, butylene glycol, ethylhexylglycerin, and disodium EDIT. .

The cosmetic composition according to an embodiment further comprises meadowfoam seed oil and safflower seed oil,

Based on 100 parts by weight of the jojoba oil, 100 parts by weight of the meadowfoam seed oil and 20 to 40 parts by weight of the safflower seed oil may be included.

The cosmetic composition according to an embodiment, with respect to 100% by weight of the total cosmetic composition, glycerin 0.1% to 10% by weight, 1,2-hexanediol 0.1% to 10% by weight, butylene glycol 0.1% by weight to 5% by weight, 0.0001% by weight to 0.1% by weight of ethylhexylglycerin, and 0.001% by weight to 1% by weight of disodium EDIT.

The cosmetic composition according to an embodiment, the cosmetic composition, mugwort leaf extract, wild strawberry leaf extract, lavender oil, and Matricaria? Further comprising, wherein the hyaluronic acid mixture includes medium-molecular hyaluronic acid and low-molecular hyaluronic acid, based on 100% by weight of the total cosmetic composition, 2.5% by weight to 3.5% by weight of jojoba oil, 0.1% by weight to 1% by weight of mugwort leaf extract , wild strawberry leaf extract 0.001 wt% to 0.01 wt%, lavender oil 0.001 wt% to 0.01 wt%, Matricaria flower oil 0.0001 wt% to 0.001 wt%, and hyaluronic acid mixture 0.001 wt% to 1 wt% have.

In the cosmetic composition according to an embodiment, the low molecular weight hyaluronic acid includes hyaluronic acid and hydrolyzed hyaluronic acid, the medium molecular weight hyaluronic acid includes sodium hyaluronate, and the low molecular weight hyaluronic acid includes The content of hyaluronic acid is twice that of the medium molecular weight hyaluronic acid, and may be included in an amount of 0.01 wt% to 1 wt% based on 100 wt% of the total cosmetic composition.

The cosmetic composition according to an embodiment may be in the form of any one or more of toner, soothing gel, lotion, essence, cream, foam cleansing, powder, pack, and mask.

In Examples, the cosmetic composition has an effect of soothing the skin and improving troubles.

The effects of the present invention are not limited to the above-mentioned effects. It should be understood that the effects of the present invention include all effects that can be inferred from the following description.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. However, since various changes may be made to the embodiments, the scope of the patent application is not limited or limited by these embodiments. It should be understood that all modifications, equivalents and substitutes for the embodiments are included in the scope of the rights.

Specific structural or functional descriptions of the embodiments are disclosed for purposes of illustration only, and may be changed and implemented in various forms. Accordingly, the embodiments are not limited to the specific disclosure form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical spirit.

Although terms such as first or second may be used to describe various elements, these terms should be interpreted only for the purpose of distinguishing one element from another. For example, a first component may be termed a second component, and similarly, a second component may also be termed a first component.

When a component is referred to as being “connected to” another component, it may be directly connected or connected to the other component, but it should be understood that another component may exist in between.

The terms used in the examples are used for the purpose of description only, and should not be construed as limiting. The singular expression includes the plural expression unless the context clearly dictates otherwise. In this specification, terms such as "comprise" or "have" are intended to designate that a feature, number, step, operation, component, part, or a combination thereof described in the specification exists, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in a variety of different forms, and only these embodiments allow the disclosure of the present invention to be complete, and common knowledge in the technical field to which the present invention belongs It is provided to fully inform the possessor of the scope of the invention, and the present invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. have meaning Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in an embodiment of the present invention, an ideal or excessively formal meaning is not interpreted as

The shapes, sizes, proportions, angles, numbers, etc. disclosed in the drawings for explaining the embodiments of the present invention are illustrative and the present invention is not limited to the illustrated matters. In addition, in describing the present invention, if it is determined that a detailed description of a related known technology may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. When 'including', 'having', 'consisting', etc. mentioned in this specification are used, other parts may be added unless 'only' is used. When a component is expressed in the singular, cases including the plural are included unless otherwise explicitly stated.

In interpreting the components, it is construed as including an error range even if there is no separate explicit description.

The size and thickness of each component shown in the drawings are illustrated for convenience of description, and the present invention is not necessarily limited to the size and thickness of the illustrated component.

Each feature of the various embodiments of the present invention may be partially or wholly combined or combined with each other, and as those skilled in the art will fully understand, technically various interlocking and driving are possible, and each embodiment may be implemented independently of each other, It may be possible to implement together in a related relationship.

Unless otherwise specified, all numbers, values, and/or expressions expressing ingredients, reaction conditions, and amounts of ingredients used herein refer to a variety of measures that may occur in obtaining such values, among others, in which such numbers are inherently different. Since they are approximations reflecting uncertainty, it should be understood as being modified by the term “about” in all cases. Also, when numerical ranges are disclosed in this description, such ranges are continuous and inclusive of all values from the minimum to the maximum inclusive of the range, unless otherwise indicated. Furthermore, when such ranges refer to integers, all integers inclusive from the minimum to the maximum inclusive are included, unless otherwise indicated.

In this specification, when a range is described for a variable, the variable will be understood to include all values within the stated range including the stated endpoints of the range. For example, a range of “5 to 10” includes the values of 5, 6, 7, 8, 9, and 10, as well as any subranges such as 6 to 10, 7 to 10, 6 to 9, 7 to 9, etc. It will be understood to include any value between integers that are appropriate for the scope of the recited range, such as 5.5, 6.5, 7.5, 5.5 to 8.5 and 6.5 to 9, and the like. Also for example, ranges from "10% to 30%" include values of 10%, 11%, 12%, 13%, etc. and all integers up to and including 30%, as well as 10% to 15%, 12% to It will be understood to include any subranges such as 18%, 20% to 30%, etc., as well as any value between reasonable integers within the scope of the recited ranges, such as 10.5%, 15.5%, 25.5%, and the like.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is the broadest concept including not only tissues covering the body surface such as the face or body, but also the scalp and hair.

As used herein, the term "improvement" refers to any action that at least reduces the severity of a parameter, eg, a symptom, associated with alleviation or treatment of a condition.

Hereinafter, the present invention will be described in detail.

In one aspect of the present invention, in a cosmetic composition having improved skin regeneration and skin moisturizing effects, jojoba oil, hyaluronic acid mixture, glycerin, 1,2-hexanediol, butylene glycol, ethylhexylglycerin and disodium EDIT Provided are a cosmetic composition containing A and a method for manufacturing the same.

In a specific embodiment of the present invention, the composition may be a cosmetic composition for soothing the skin.

In a specific embodiment of the present invention, the composition may be a cosmetic composition for improving skin troubles.

In the present invention, the "jojoba oil (jojoba oil, Simmondsia chinensis )" is an unsaturated alcohol extracted from jojoba seeds. Jojoba is a desert shrub native to southern Mexico, California, and Israel. Jojoba oil has a molecular structure similar to that of human sebum, so it can be absorbed better by the human body. The crude one is clear yellow, but the refined jojoba oil is colorless and odorless. It is regarded as the most stable oil among natural oils. Also, compared to other natural oils, it has a light feel and is less sticky. Unlike common vegetable oils, jojoba oil is in the form of a liquid wax, which solidifies when the temperature is low and turns into a liquid when the temperature increases.

The present inventors found that the cosmetic composition containing jojoba oil, hyaluronic acid mixture, glycerin, 1,2-hexanediol, butylene glycol, ethylhexyl glycerin and disodium EDTA exhibits skin soothing and skin trouble improvement effects. The present invention was completed by finding the facts.

The cosmetic composition may further include meadowfoam seed oil and safflower seed oil.

In particular, in the present invention, when a composition in which jojoba oil, meadowfoam seed oil, and safflower seed oil are mixed in a specific composition ratio is used, skin soothing and skin trouble improvement effect than a composition containing some of these components or a composition containing other composition ratios It was experimentally proven that the significantly improved (see Test Examples 1 and 2).

In this case, based on 100 parts by weight of the jojoba oil, 100 parts by weight of the meadowfoam seed oil, 10 to 100 parts by weight of the safflower seed oil, and more specifically, 20 to 40 parts by weight of the safflower seed oil may be included. The mixed oil may be prepared by mixing after preparing each oil, or by mixing safflower and meadowfoam and then extracting the primary mixed oil, and then mixing separately prepared jojoba oil to make a secondary mixed oil. .

In a specific embodiment of the present invention, the mixed oil is contained in an amount of 0.01 to 10% by weight based on 100% by weight of the total cosmetic composition. Specifically, the jojoba oil may be included in an amount of 2.5 wt% to 3.5 wt%. The jojoba oil, meadowfoam seed oil and safflower seed oil can soothe the skin and improve skin troubles.

In a specific embodiment of the present invention, based on 100% by weight of the total cosmetic composition, glycerin 0.1% to 10% by weight, 1,2-hexanediol 0.1% to 10% by weight, butylene glycol 0.1% by weight % to 5% by weight, 0.0001% to 0.1% by weight of ethylhexylglycerin, and 0.001% to 1% by weight of disodium EDIT.

In a specific embodiment of the present invention, in the cosmetic composition, the natural extract includes mugwort leaf extract and wild strawberry leaf extract, and the hyaluronic acid mixture includes medium molecular weight hyaluronic acid and low molecular weight hyaluronic acid, and the cosmetic composition The composition further comprises lavender oil and Matricaria flower oil, with respect to 100% by weight of the total cosmetic composition, 2.5% by weight to 3.5% by weight of jojoba oil, 0.1% by weight to 1% by weight of mugwort leaf extract, wild strawberry leaf Extract 0.001 wt% to 0.01 wt%, lavender oil 0.001 wt% to 0.01 wt%, Matricaria flower oil 0.0001 wt% to 0.001 wt%, glycerin 0.1 wt% to 10 wt%, 1,2-hexanediol 0.1 wt% % to 10% by weight, butylene glycol 0.1% to 5% by weight, ethylhexylglycerin 0.0001% to 0.1% by weight, disodium EDTA 0.001% to 1% by weight, and hyaluronic acid mixture 0.001% to 1 Including weight %, the content of the low molecular weight hyaluronic acid may be greater than the content of the medium molecular weight hyaluronic acid. In such a composition and content, in particular, the fibrous trouble can be improved, and the skin soothing effect can be greatly improved. In particular, since the content of low-molecular hyaluronic acid is greater than that of medium-molecular hyaluronic acid, it is possible to supply moisture deep into the skin.

In a specific embodiment of the present invention, the low molecular weight hyaluronic acid includes hyaluronic acid and hydrolyzed hyaluronic acid, and the medium molecular weight hyaluronic acid includes sodium hyaluronate, The low molecular weight hyaluronic acid and the medium molecular weight hyaluronic acid are mixed in a weight ratio of 2: 1, and may be included in an amount of 0.01% to 1% by weight based on 100% by weight of the total cosmetic composition.

In one specific embodiment of the present invention, with respect to the cosmetic composition, 0.1 wt% to 25 wt% of olive oil, 0.1 wt% to 10 wt% of propanediol, 0.1 wt% to 10 wt% of glycereth-26, methyl Gluceth-20 0.1 wt% to 10 wt%, sodium chloride 0.1 wt% to 10 wt%, bergamot oil 0.000001 wt% to 0.1 wt%, perilla extract 0.0001 wt% to 0.1 wt%, sodium citrate 0.01 wt% to 1 % by weight, citric acid 0.001% to 1% by weight, orange oil 0.0001% to 1% by weight, wild geranium oil 0.00001% to 1% by weight, bergamot oil 0.00001% to 1% by weight, frankincense oil 0.0001% by weight to 1 wt%, rose flower oil 0.00001 wt% to 1 wt%, benzoin wood gum 0.0000001 wt% to 1 wt%, and myrrh oil 0.000001 wt% to 1 wt% may be further included. When the content of each component satisfies the above numerical range, the cosmetic composition has excellent skin regeneration and skin moisturizing effects.

In the present invention, the "meadowfoam seed oil ( Limnanthes alba (Meadowfoam) seed oil)" is a seed oil extracted from the seeds of Limnanthes alba . Meadow seeds contain 20-30% of oil, and meadowfoam seed oil contains 98 % or more of long-chain fatty acids, so it is very stable.

In the present invention, the "safflower seed oil ( Carthamus tinctorius seed oil)" is an oil obtained by pressing the seeds of a safflower plant. The content of linoleic acid, considered an essential fatty acid, is very high (62-72%), followed by oleic acid (16-25%) and linolenic acid (1-6%) in that order.

In the present invention, the jojoba oil, meadowfoam seed oil and safflower seed oil can be prepared by the following method.

First, seeds are separated from jojoba and the separated seeds are washed with water. The washed seeds are dried to control the moisture contained in the seeds within a range of 5 to 20 wt%, and the dried seeds are pulverized by pulverizing them with a grinder. Then, the pulverized seed powder is cold-pressed to milk the seed oil. Thereafter, the seed oil extracted in the milking process is filtered through a filter to obtain pure oil. In this way, pure oil can be obtained without adding heat or adding a chemical solvent.

Next, the seeds are separated from the meadowfoam or safflower, and the separated seeds are washed with water. The washed seeds are dried to control the moisture contained in the seeds within the range of 5 to 20% by weight, and the dried seeds are pulverized by pulverizing them with a grinder. Then, high-temperature steam is applied to the pulverized seed powder to humidify and heat the seed powder. At this time, the time for applying steam from the steamer may be adjusted within the range of about 5 minutes to 30 minutes. If excessive humidification is made, the fiber of the seed powder may absorb moisture and cause a problem in that the volume increases and agglomerates. Through this humidification and heating process, the oil in the seeds is melted by the hot steam, so that the milking process can be carried out smoothly. In this way, the heated and humidified seed powder is pressed and milked. The seed oil extracted during the milking process is filtered through a filter to obtain pure oil. In this way, it is possible to manufacture high-purity seed oil with high efficiency.

In the present invention, the "wormwood leaf extract" includes wormwood, and wormwood ( Artemisia annua L. ) is an annual herb belonging to the Asteraceae and is native to tropical Asia and is distributed worldwide including Korea. It is used as an antipyretic, hemostatic, skin disease treatment, insecticide, etc.

In the present invention, the "wild strawberry leaf extract" is extracted from the leaves of wild strawberries (Strawberry Fragaria vesca) and can be used as a skin conditioning agent.

In the present invention, 'extract' means that the extract is separated and obtained from the active ingredient raw material using a method known in the art for extraction and separation, and the extract as defined in the present invention is prepared by using an appropriate solvent. It may be extracted by pulverizing or as it is the active ingredient prepared as a raw material. In this case, as a suitable solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and water or an organic solvent may be used. For example, an extraction solvent such as water, an alcohol having 1 to 5 carbon atoms, hexane, chloroform, or butylene glycol may be used alone or in combination, but is not limited thereto.

As the extraction method, methods such as hot water extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression can be used. In addition, the desired extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. There is no limitation on the method for preparing the extract of the present invention, and any known method may be used.

In the present invention, the "hyaluronic acid" is one of the natural moisturizing factors (NMF) that are widely used for skin moisturizing. In the case of hyaluronic acid, it is divided into low molecular weight, medium molecular weight, and high molecular weight according to its size.

High molecular weight hyaluronic acid has a molecular weight of about 1,500,000 Da or more and typically includes sodium hyaluronate crosspolymer, hydrolyzed glycosaminoglycans, etc. Hyaluronic acid has a molecular weight of about 1,000,000 to 15,000 Da, and is typically sodium hyaluronate, hydroxypropyltrimonium hyaluronate, sodium acetylated hyaluronate (Sodium). Acetylated Hyaluronate) and the like, and low molecular weight hyaluronic acid has a molecular weight of approximately 5,000 to 100,000 Da, and potassium hyaluronate, hydrolyzed hyaluronic acid, hydrolyzed sodium Hyaluronate (Hydrolyzed Sodium Hyaluronate), hyaluronic acid (Hyaulonic Acid), and the like.

The high molecular weight, medium molecular weight, and low molecular weight hyaluronic acid is divided according to the size of the molecular weight, and any hyaluronic acid falling within the molecular weight range may be included in the high molecular weight, medium molecular weight, or low molecular weight hyaluronic acid without limitation.

The principle of action of the hyaluronic acid to moisturize the skin is different depending on the molecular size. For example, high molecular weight hyaluronic acid forms a moisturizing film on the stratum corneum and has effects such as water retention and moisture evaporation prevention. Medium molecular weight hyaluronic acid has an excellent effect of providing a moisturizing function to the outer skin while acting on the epidermal layer, and low molecular weight hyaluronic acid is similarly It provides moisture to the epidermal layer and relieves the feeling of dryness in the skin. In addition, in the case of ultra-low molecular weight hyaluronic acid, it provides moisture to the dermis layer and increases moisture in the skin, thereby having an excellent effect in preventing skin aging.

In one embodiment of the present invention, the composition may be a cosmetic composition. The cosmetic composition is not particularly limited in formulation, and may be appropriately selected according to the purpose. For example, it may be prepared in any one formulation of toner, soothing gel, lotion, essence, cream, foam cleansing, powder, pack, and mask, but is not limited thereto.

In one embodiment of the present invention, the cosmetic composition may further include a functional additive and a component included in a general cosmetic composition in addition to the added active ingredient. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

In one embodiment of the present invention, the cosmetic composition of the present invention may further contain a component contained in a general cosmetic composition, if necessary, together with the functional additive.

However, it is characterized in that it does not contain paraben-based components, talc and artificial colors. Accordingly, it is possible to provide a cosmetic composition with reduced skin adverse reactions.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

Preparation Example 1: Preparation of Jojoba Seed Oil

After separating only the seeds from the jojoba fruit and washing 100 g of the collected jojoba seeds, the seeds were dried for 3 days to adjust the moisture content to 15% by weight, and then the seeds were pulverized to prepare a powder. The powder was compressed by cold pressing to extract seed oil, and filtered through a filtration filter to prepare jojoba seed oil.

Preparation Example 2: Preparation of Meadowfoam Seed Oil

After separating only the seeds from the meadowfoam fruits, 100 g of the collected meadowfoam seeds were washed, the seeds were dried in the shade for 3 days, the moisture content was adjusted to 15% by weight, and then the seeds were pulverized to prepare a powder. After steam was applied to the powder in a steamer for 30 minutes, the powder was put into a hydraulic milking machine to extract seed oil, and then filtered through a filtration filter to prepare meadowfoam seed oil.

Preparation Example 3: Preparation of safflower seed oil

After separating only the seeds from the safflower fruit and washing 100 g of collected safflower seeds, the seeds were dried in the shade for 3 days to adjust the moisture content to 15% by weight, and then the seeds were pulverized to prepare a powder. After steam was applied to the powder in a steamer for 30 minutes, the powder was put into a hydraulic milking machine to extract seed oil, and safflower seed oil was prepared by filtration through a filter.

Test Example 1: Preparation of mixed oil of jojoba seed oil, meadowfoam seed oil or safflower seed oil

A mixed oil containing jojoba seed oil, meadowfoam seed oil, or safflower seed oil prepared in Preparation Examples 1 to 3 was prepared according to the composition shown in Table 1 below.

At this time, each oil was mixed with the composition of Table 1 below, and the oils were transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

division weight ratio Jojoba Seed Oil Meadow Foam Seed Oil Safflower Seed Oil Example 1 One One One Comparative Example 1 3 0 0 Comparative Example 2 0 3 0 Comparative Example 3 0 0 3 Comparative Example 4 2 One 0

Experimental Example 1: Cytotoxicity test

By checking the cell growth rate by concentration with jojoba seed oil, meadowfoam seed oil and safflower seed oil before entering the efficacy test, the appropriate test sample concentration is calculated and a cytotoxicity test as a confirmation test whether the concentration to be used affects safety was carried out.

The cells used in the experiment were HaCaT (Human Skin Keratinocytes cell line) and Dulbecco's modified Eagle's medium supplemented with 37°C, 5% CO2, 10% Fetal bovine serum (FBS, Lonza), and 50μg/mL streptomycin (Sigma). (DMEM, Invitrogen). After culturing, the medium was removed, and the mixed oil prepared in Example was diluted with dimethyl sulfoxide to a final concentration of 100, 500, 1000, and 5000 μg/ml, treated with a diluted solution and cultured for 24 hours, then MTT (3 - 100 μl of [4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium boromide, Sigma, USA) solution was added to each well (3 mg/ml) and incubated for 4 hours more. Then, the supernatant was removed, 150 μl of dimethyl sulfoxide was added, and the resulting formazan was dissolved by shaking for 30 minutes, and absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to the following formula, and the results are shown in Table 2 below.

Cell viability (%) = absorbance of the sample added group / absorbance of the control group × 100.

Sample concentration (μg/μl) Cell viability (%) Jojoba Seed Oil Meadow Foam Seed Oil Safflower Seed Oil Jojoba seed oil, meadowfoam seed oil and safflower seed oil blended oil control 100 100 100 100 100 100 500 100 100 100 100 1000 100 100 100 100 5000 99.50 99.40 99.35 99.52

As can be seen from the results in Table 2 above, as all samples to which jojoba seed oil, meadowfoam seed oil, safflower seed oil, and mixed oils thereof were applied, cell viability of more than 99% was confirmed, so that cytotoxicity was not affected. Able to know. Accordingly, it was confirmed that jojoba seed oil, meadowfoam seed oil and safflower seed oil had no safety problem.

Test Example 2: Preparation of softened lotion

As shown in Table 3 below, a softening lotion was prepared for the optimized trouble improvement effect test of the cosmetic composition of a mixed oil containing jojoba oil, meadowfoam seed oil and safflower seed oil of the present invention.

ingredient weight% Example 2-1 Example 2-2 Example 2-3 Example 2-4 Example 2-5 Example 2-6 Example 2-7 Example 2-8 Example 2-9 Example 2-10 Comparative Example 5 glycerin 2.0895 olive oil 20.91 Disodium EDIT 0.014 sodium citrate 0.07 Jojoba Seed Oil One One One One One One One One One One 0 Meadow Foam Seed Oil One One One One One One One One One One 0 Safflower Seed Oil 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 0 mugwort leaf extract 0.245 wild strawberry leaf extract 0.0042 lavender oil 0.00189 matrimaria flower oil 0.00018 Hyaluronic Acid 0.007 Hydrolyzed Hyaluronic Acid 0.007 Sodium Hyaluronate 0.007 Fragrance, preservatives, etc. a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount total 100 100 100 100 100 100 100 100 100 100 100

Experimental Example 2: Evaluation of skin soothing effect

The experiment was conducted on 20 adult females between the ages of 19 and 60 who had no skin diseases that could affect the experimental results. Specifically, 20 women (average age 42.3 years) were selected according to the following selection criteria, and the facial region of the test subject was selected as the test site. After cleansing twice a day in the morning and evening for 4 weeks during the test period, the cosmetic composition, which is the test substance, was evenly spread on the face for absorption. Essence, whitening cream, anti-aging cream, etc. The use of the same functional cosmetics and exfoliants was prohibited.

The skin soothing effect was evaluated by applying a spectrophotometer (Spectrophotometer CM-2600D, Konica Minolta, Japan) and an ophthalmic imaging system VISIA-CA (VISIA Complexion Analysis, Canfield Scientific, Inc., USA). The spectrophotometer was used for analysis by measuring the left cheek area of all subjects three times consecutively by the same test person and calculating the average value thereof. The measurement value of the spectrophotometer consists of three factors, L*, a*, and b*. L* value is brightness, a* value is redness, and b* value is yellowness. indicates As a value to measure the skin soothing effect of the test substance, the a* value indicating the redness of the skin was used for the analysis, and as the measured value decreases compared to before the use of the test substance, it means that there is a skin soothing effect. The VISIA-CA, an all-eye imaging system, measured the facial regions of all subjects by the same investigator in the same position with uniform illumination for consistent imaging. In order to secure the hairline of the subject's forehead, the hair was fixed with a hair band, and the same top gown was worn and the subject was seated on a chair, and the front of the face was photographed while the face was fixed on the top of the toolbar under the cage. Instrument measurements were made before the use of the test substance and after 4 weeks of use.

Table 4 below shows the results of evaluating the skin soothing effect before and after 4 weeks of use of the test substance using a spectrophotometer and an entire ocular imaging system VISIA-CA.

division a* value change a* value improvement (%) a* value statistical analysis average Standard Deviation Example 2-3 before use 12.04 1.45 - after 4 weeks of use 11.54 0.96 4.18% 0.013 a* value improvement rate (%) = [measured value after use - measured value before use]/measured value before use × 100 (%)
*p <0.05, **p < 0.01, ***p < 0.0001: p-value is measured by paired t-test

Experimental Example 3: NO production inhibitory effect

In order to investigate the anti-inflammatory effect of the cosmetic composition of the mixed oil containing jojoba oil, meadowfoam seed oil and safflower seed oil of the present invention, the NO production inhibitory effect was confirmed. Mouse macrophages, RAW 264.7 cells (ATCC number: CRL-2278) were dispensed in a 60 mm plate at a density of 2 X 106 cells/dish and cultured on the plate for 24 hours. In order to induce an inflammatory response, a fresh medium containing LPS (lipopolysaccharide) at a concentration of 1 μg/ml and a test substance was treated and cultured for 24 hours. Anti-inflammatory activity evaluation through NO assay was performed using griess A and B reagents, and 1% sulfanilamide and 0.1% N-(1-naphtyl)ethylenediamine dihydrochloride reagent were mixed in a 1:1 ratio. After processing Examples and Comparative Examples in a 96 well plate, _{the supernatant of the medium cultured for 24 hours at 37° C. and 5% CO 2} was mixed with griess (A+B) reagent at a 1:1 ratio by 50 μl each. After storage at room temperature for 10 minutes, the NO production inhibitory effect was measured by ELISA measurement at 540 nm. The NO production inhibition rate (%) was calculated using Equation 1 below and shown in Table 5, and the experiment was performed three times, and the average value was expressed.

[Equation 1]

NO production inhibition rate (%)={(absorbance of negative control group - absorbance of each extract)/absorbance of negative control} × 100

division NO production inhibition rate (%) Example 2-1 48.8 Example 2-2 65.7 Example 2-3 68.2 Example 2-4 66.9 Example 2-5 48.3 Example 2-6 48.7 Example 2-7 50.2 Examples 2-8 56.0 Examples 2-9 48.2 Example 2-10 48.6 Example 1 48.6 Comparative Example 1 18.2 Comparative Example 2 16.8 Comparative Example 3 23.8 Comparative Example 4 32.9 Control (DMSO, 10 ppm) -

As shown in Table 5, the samples of Examples 2-1 to 2-10 were all confirmed to have significantly improved NO production inhibition rate compared to Comparative Examples 1-4. In particular, the samples of Examples 2-2 to 2-4 showed a significantly high NO production inhibition rate.

Experimental Example 4: Measurement of skin trouble improvement effect

In order to verify the skin trouble improvement effect, the following experiment was performed. The cosmetic composition of Example 2-2 was applied to the face and back of 10 adolescent experimenters with a lot of acne once a day for 4 weeks. After 30 days, by changing the number of acne on the face and back, the effect of improving skin troubles was checked, and the results are shown in Table 6 below.

division Number of pimples immediately after application Number of acne after 4 weeks experimenter 1 25 17 experimenter 2 49 29 experimenter 3 46 23 experimenter 4 37 21 experimenter 5 44 26 experimenter 6 36 18 experimenter 7 53 40 experimenter 8 65 55 experimenter 9 72 48 experimenter 10 24 16

Looking at Table 6, although there are individual differences, it can be confirmed that the skin trouble improvement effect is improved overall.

Experimental Example 5: Inhibitory effect of inflammatory cytokine TNF-α expression

The inhibitory effect of the inflammatory cytokine TNF-α expression of the cosmetic composition of the mixed oil containing jojoba oil, meadowfoam seed oil and safflower seed oil of the present invention was confirmed. RAW 264.7 cells, which are mouse macrophages, ^{were adjusted to 5×10 5} cells/well using DMEM medium supplemented with 10% FBS, inoculated in a 12 well plate, and cultured for 18 hours. After maintaining the starvation state for 12 hours after incubation, LPS 10ng/mL and Examples and Comparative Examples were treated and cultured for 24 hours. RNA was extracted from cultured cells and the expression rate of TNF-α was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The TNF-α expression rate was calculated by Equation 2 below, and the TNF-α expression inhibition rate refers to a value obtained by subtracting the TNF-α expression rate from 100%.

[Equation 2]

TNF-α expression rate (%) = (sample TNF-α expression amount / control TNF-α expression amount) × 100.

The results are shown in Table 7 below.

division TNF-α expression inhibition rate (%) Example 2-1 43.8 Example 2-2 55.7 Example 2-3 56.1 Example 2-4 58.2 Example 2-5 45.2 Example 2-6 41.2 Example 2-7 42.2 Examples 2-8 44.5 Examples 2-9 47.6 Example 2-10 43.0 Example 1 43.0 Comparative Example 1 25.2 Comparative Example 2 15.2 Comparative Example 3 26.9 Comparative Example 4 20.2 Control (DMSO, 10 ppm) -

As shown in Table 7, the samples of Examples 2-1 to 2-10 all showed significantly improved TNF-α expression inhibition compared to Comparative Examples 1-4. In particular, the samples of Examples 2-2 to 2-4 showed a remarkably high TNF-α expression inhibition rate.

Formulation Example 1: Preparation of flexible lotion

Jojoba oil, meadowfoam seed oil and safflower seed oil according to an aspect of the present invention can be prepared into various types of cosmetics depending on the purpose. The following exemplifies a method for preparing a softening lotion containing jojoba oil, meadowfoam seed oil and safflower seed oil as an active ingredient according to an aspect of the present invention, but the present invention is not limited thereto.

Purified water 54.026 wt%

20.91 wt% olive oil

5.6 wt% propanediol

Glycereth-26 3.5 wt%

Safflower seed oil 3.0 wt%

Meadowfoam Seed Oil 3.0 wt%

Jojoba seed oil 3.0 wt%

glycerin 2.0895 wt%

Purified water 0.0105 wt%

1.40 wt% of 1,2-hexanediol

Butylene glycol 0.7 wt%

0.679 wt% propanediol

0.007% by weight of hyaluronic acid

Hydrolyzed hyaluronic acid 0.007 wt%

Sodium Hyaluronate 0.007 wt%

Methylglucose-20 0.7 wt%

Sodium Chloride 0.7 wt%

Artemisia leaf extract 0.245 wt%

butylene glycol 0.105 wt%

Bergamot oil 0.081 wt%

Butylene glycol 0.0483 wt%

purified water 0.021 wt%

0.0007 wt% of perilla extract

Sodium Citrate 0.07 wt%

0.035 wt% of ethylhexylglycerin

citric acid 0.028 wt%

Disodium EDIT A 0.014 wt%

Orange oil 0.00551 wt%

Lavender oil 0.00189 wt%

Wild Geranium Oil 0.00045 wt%

Bergamot oil 0.00041 wt%

0.00032% by weight of dandelion oil

Rose flower oil 0.00023 wt%

Matricaria flower oil 0.00018 wt%

0.00001 wt% of benzoin wood gum

Myrrh oil 0.00001 wt%

Wild strawberry leaf extract 0.00420 wt%

Butylene glycol 0.00280 wt%

Formulation Example 2: Preparation of soft lotion

Jojoba oil, meadowfoam seed oil and safflower seed oil according to an aspect of the present invention can be prepared into various types of cosmetics depending on the purpose. The following exemplifies a method for preparing a softening lotion containing jojoba oil, meadowfoam seed oil and safflower seed oil as an active ingredient according to an aspect of the present invention, but the present invention is not limited thereto.

56.126 wt% of purified water

20.91 wt% olive oil

5.6 wt% propanediol

Glycereth-26 3.5 wt%

Safflower seed oil 0.9 wt%

Meadowfoam Seed Oil 3.0 wt%

Jojoba seed oil 3.0 wt%

glycerin 2.0895 wt%

Purified water 0.0105 wt%

1.40 wt% of 1,2-hexanediol

Butylene glycol 0.7 wt%

0.679 wt% propanediol

0.007% by weight of hyaluronic acid

Hydrolyzed hyaluronic acid 0.007 wt%

Sodium Hyaluronate 0.007 wt%

Methylglucose-20 0.7 wt%

Sodium Chloride 0.7 wt%

Artemisia leaf extract 0.245 wt%

butylene glycol 0.105 wt%

Bergamot oil 0.081 wt%

Butylene glycol 0.0483 wt%

purified water 0.021 wt%

0.0007 wt% of perilla extract

Sodium Citrate 0.07 wt%

0.035 wt% of ethylhexylglycerin

citric acid 0.028 wt%

Disodium EDIT A 0.014 wt%

Orange oil 0.00551 wt%

Lavender oil 0.00189 wt%

Wild Geranium Oil 0.00045 wt%

Bergamot oil 0.00041 wt%

0.00032% by weight of dandelion oil

Rose flower oil 0.00023 wt%

Matricaria flower oil 0.00018 wt%

0.00001 wt% of benzoin wood gum

Myrrh oil 0.00001 wt%

Wild strawberry leaf extract 0.00420 wt%

Butylene glycol 0.00280 wt%

In the above, embodiments of the present invention have been described with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains can practice the present invention in other specific forms without changing its technical spirit or essential features. You can understand that there is Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (3)

As a cosmetic composition comprising jojoba oil, a mixture of hyaluronic acid, glycerin, 1,2-hexanediol, butylene glycol, ethylhexylglycerin and disodium EDI,
The cosmetic composition further comprises meadowfoam seed oil and safflower seed oil,
The cosmetic composition further comprises mugwort leaf extract, wild strawberry leaf extract, lavender oil and Matricaria flower oil,
The hyaluronic acid mixture includes medium molecular weight hyaluronic acid and low molecular weight hyaluronic acid,
The medium molecular weight hyaluronic acid is sodium hyaluronate,
The low molecular weight hyaluronic acid is hyaluronic acid or hydrolyzed hyaluronic acid,
Based on 100 parts by weight of the jojoba oil, 100 parts by weight of the meadowfoam seed oil and 20 to 40 parts by weight of the safflower seed oil are included,
With respect to 100% by weight of the total cosmetic composition,
2.5% to 3.5% by weight of jojoba oil;
Artemisia leaf extract 0.1 wt% to 1 wt%,
0.001 wt% to 0.01 wt% of wild strawberry leaf extract,
0.001 wt% to 0.01 wt% of lavender oil,
Matricaria flower oil 0.0001 wt% to 0.001 wt%,
0.1% to 10% by weight of glycerin,
0.1% to 10% by weight of 1,2-hexanediol,
butylene glycol 0.1 wt% to 5 wt%,
0.0001 wt% to 0.1 wt% of ethylhexylglycerin;
0.001 wt% to 1 wt% of disodium EDIT; and
A cosmetic composition for soothing skin and improving troubles, comprising 0.001% to 1% by weight of a hyaluronic acid mixture.

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