KR102576693B1 - Cosmetic composition comprising infused oils of calendular and mixed extracts of plants for moisturizing skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing skin containing calendula infuse oil and mixed plant extract as active ingredients, and specifically, calendula infuse oil prepared by cold immersion using vegetable oil, and astragalus, Cattle and It relates to a cosmetic composition having an excellent moisturizing effect containing a mixed extract of sea buckthorn fruit.

Description

Cosmetic composition for moisturizing skin containing calendula infuse oil and mixed plant extract as active ingredients {COSMETIC COMPOSITION COMPRISING INFUSED OILS OF CALENDULAR AND MIXED EXTRACTS OF PLANTS FOR MOISTURIZING SKIN}

The present invention relates to a method for producing a skin moisturizing cosmetic composition containing calendula infuse oil and mixed plant extract as active ingredients. Specifically, calendula infuse oil prepared by cold immersion using vegetable oil, and astragalus astragalus It relates to a method for producing a cosmetic composition with excellent moisturizing effect, including mixed extracts of wood and sea buckthorn fruits.

The most important function of the epidermis, located in the outermost layer of the skin, is to defend against various external stimuli (physical and chemical irritants such as chemicals, air pollutants, dry environments, ultraviolet rays, etc.) and to protect the skin. It is a protective function that prevents excessive dissipation of moisture in the body through the skin, and this protective function can be maintained only when the stratum corneum, composed of keratinocytes, is normally formed.

The stratum corneum, the outermost layer of the epidermis, is formed from keratinocytes and consists of fully differentiated keratinocytes and a lipid layer surrounding them. Keratinocytes are characteristic cells that rise to the surface of the skin as basal cells that continuously proliferate in the lowest layer of the epidermis undergo gradual changes in form and function. After a certain period of time, old keratinocytes are removed from the skin. They fall off and new keratinocytes take over their function. This repetitive series of changes is called “epidermal cell differentiation” or “keratinization.” During this keratinization process, keratinocytes produce Natural Moisturizing Factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acids) to form a stratum corneum, making the stratum corneum firm and flexible, forming a skin barrier. It functions as a barrier.

However, this stratum corneum can easily lose its function due to lifestyle factors such as excessive washing or bathing, environmental factors such as dry air and pollutants, and endogenous diseases such as atopic skin or senile skin. In fact, due to various factors that have increased in modern times, the number of people complaining of dry skin symptoms and various disorders resulting from this is increasing. Therefore, in order to maintain appropriate skin moisture, much research has been conducted to supply moisture from outside or prevent moisture loss from the body, and various types of moisturizers with actual moisture retention ability have been developed, mainly It is widely used in the cosmetics field.

Various prior literature is known to improve skin moisturization by using natural extracts or oils prepared by the infuse method.

Korean Patent Publication No. 2019-0001530 discloses a composition for relieving and improving atopic dermatitis containing jojoba oil, calendula oil, sweet almond oil, chamomile oil, jasmine oil, etc.

Korean Patent Publication No. 2021-0037338 discloses a method for manufacturing infused oil that can effectively shorten the extraction period. The above patent uses lecithin, an emulsifier, and the manufactured infused oil reduces transepidermal water loss.

The present invention is intended to provide a method for producing calendula infuse oil and a cosmetic composition for skin moisturizing that includes mixed extracts of Astragalus, Astragalus and sea buckthorn fruits, and has excellent skin moisturizing effects and skin barrier improvement effects.

The above task includes mixing dried calendula and green tea oil at a weight ratio of 1:8 to 10 and maintaining it at room temperature for 20 to 40 days to prepare calendula infused oil; Preparing a mixed extract of astragalus, ash tree, and sea buckthorn fruit by mixing dried astragalus, ash tree, and sea buckthorn fruit in a weight ratio of 1:1:1 and then extracting them with an organic solvent; and mixing the calendula infuse oil and the mixed extract at a weight ratio of 10 to 100:1.

Preferably, the green tea oil can be produced by drying and powdering green tea seeds and then using steam distillation.

Also preferably, the organic solvent is anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids, and these. It may be selected from the group consisting of mixtures.

Also preferably, the mixture of the calendula infuse oil and the mixed extract includes beeswax, vitamin E, and at least one essential oil selected from the group consisting of chamomile essential oil, lavender French essential oil, and tea tree essential oil, It may additionally include the step of preparing a cosmetic in a balm or solid stick formulation.

Also preferably, the cosmetic composition for moisturizing the skin may further include one or more ingredients selected from the group consisting of chamomile essential oil, lavender French essential oil, tea tree essential oil, and vitamin E.

The cosmetic composition prepared by the method of the present invention has less skin irritation and has excellent skin barrier improvement and skin moisturizing effects. In addition, there is almost no specific odor from the oil components, almost no rancidity occurs, and storage is excellent.

Figure 1 is a photograph of calendula infused oil according to the present invention.
Figure 2 is a product photograph of an ointment formulation (A) and a stick formulation (B) of the cosmetic composition according to the present invention.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meaning as generally understood by a person skilled in the art in the relevant field of the present invention. In addition, preferred methods and samples are described in this specification, but similar or equivalent methods are also included in the scope of the present invention.

The term “about” means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 9, 8, 7, 6, 5, or means a quantity, level, value, number, frequency, percentage, dimension, size, volume, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, unless the context otherwise requires, the words "comprise" and "comprising" include a given step or component, or group of steps or components, but any other step or component, or It should be understood that this implies that no step or group of components is excluded.

The present invention relates to a method for producing a cosmetic composition for skin moisturizing containing calendula infuse oil and mixed extracts of astragalus, japonica and sea buckthorn fruit.

The method according to the present invention includes the step of mixing dried calendula and vegetable oil and maintaining it at room temperature for 20 to 40 days to prepare calendula infused oil (S11); A step of preparing a mixed extract of astragalus, japonica and sea buckthorn fruits by pulverizing and mixing the dried fruits, respectively, and extracting them with an organic solvent (S12); and mixing the calendula infused oil and the mixed extract (S13).

Each step is described in detail below.

First, dried calendula and vegetable oil are mixed and kept at room temperature for 20 to 40 days to prepare calendula infused oil (S11).

Preferably, the dried calendula and vegetable oil are mixed at a weight ratio of 1:8 to 10. More preferably, they can be mixed at a weight ratio of 1:10. If the weight ratio of dried calendula to vegetable oil exceeds 1:10, the oil content increases, and the content of active ingredients per unit weight decreases. If the weight ratio of dried calendula to vegetable oil is less than 1:8, the amount of calendula oil finally obtained is small compared to the weight of dried calendula, so economic feasibility is low.

Calendula (C alendula officinalis L.) , which is used as an active ingredient in the cosmetic composition of the present invention, is a double leaf plant belonging to the Asteraceae family and has red, yellow, and orange flowers and is native to southern Europe. Calendula is used as a food, dye, and pesticide, and is also used to treat inflammatory skin diseases. Calendula oil is also used as an anti-inflammatory and wound treatment agent. In the present invention, calendula flowers are used.

The vegetable oil may be selected from the group consisting of green tea oil, sweet almond oil, sunflower seed oil, camellia oil, and grape seed oil. More preferably, green tea oil can be used. Conventionally, evening primrose oil, jojoba oil, shea butter, and olive oil are used as infused oils, but these oils have a strong specific odor and are expensive, making them less economical. In comparison, green tea oil, sweet almond oil, sunflower seed oil, camellia oil, and grape seed oil have almost no specific odor, do not produce impurities when blended with calendula, and are characterized by low density. In addition, even when formulated into a final ointment or stick formulation, it has excellent spreadability, high skin absorption, and is less sticky. Therefore, the present invention is characterized by the use of green tea oil, sweet almond oil, sunflower seed oil, camellia oil, and grape seed oil.

Green tea oil is an oil extracted from green tea seeds and contains a large amount of vitamins A, B, and C, so it can reduce melanin pigmentation such as spots and freckles. Additionally, green tea oil contains a large amount of catechin components, so green tea oil By producing calendula oil, sterilization and sebum control effects can be improved and storage stability can be improved.

According to one embodiment of the present invention, the green tea oil may be manufactured by drying and powdering green tea seeds and then using steam distillation.

In the present invention, calendula oil is characterized in that it is manufactured by a cold maceration method in which dried calendula and vegetable oil are kept at room temperature, preferably in a cool place without direct sunlight for 20 to 40 days. Preferably, it can be maintained for 30 days. During the above period, stir up and down 2-3 times daily to mix the ingredients well. Once cold maceration is completed, calendula solids are removed using a strainer to produce calendula oil. In the present invention, by producing calendula oil using a cold maceration method, rancidity rarely occurs and the active ingredients of calendula and vegetable oil are less deformed.

Next, the dried Astragalus, Astragalus, and Sea Buckthorn fruits are extracted with an organic solvent to prepare a mixed extract of Astragalus, Cattle, and Sea Buckthorn fruits (S12). Preferably, the dried astragalus, astragalus and sea buckthorn fruits are mixed in a weight ratio of 1:1:1.

Astragalus membranaceus , which is used as an active ingredient in the cosmetic composition of the present invention, is a perennial herbaceous plant belonging to the legume family. Its roots are mainly used as a medicine, and its medicinal ingredients include folic acid, choline, etc. there is. In the present invention, the root of Astragalus is used.

Caesalpinia sappan , used as an active ingredient in the cosmetic composition of the present invention, is a plant belonging to the legume family and is distributed in tropical Asia, such as India, Malaysia, and southern China. In Oriental medicine, Somok is used to promote blood circulation, eliminate stagnant blood, and reduce swelling. In the present invention, the stem or leaves of the small tree can be used.

The sea buckthorn fruit used as an active ingredient in the cosmetic composition of the present invention is the fruit of the sea buckthorn tree. Sea buckthorn ( Hippophae rhamnoides ) belongs to the Rosaceae family, grows in cold regions of Europe and Asia, and is also called the vitamin tree. Sea buckthorn fruit is known to be high in vitamin C and vitamin E.

The roots of Astragalus, stems and leaves of Astragalus, and sea buckthorn fruits can be dried and pulverized, and then extracts can be prepared using an organic solvent. The organic solvent is from the group consisting of anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids, and mixtures thereof. It may be selected, preferably ethanol, and more preferably 70% (v/v) ethanol.

Finally, the calendula infuse oil and the mixed extract are mixed at a weight ratio of 10 to 100:1 to prepare a cosmetic composition for moisturizing skin according to the present invention (S13).

Additionally, according to another embodiment of the present invention, the cosmetic composition of the present invention may include additional ingredients selected from the group consisting of chamomile essential oil, lavender essential oil, tea tree essential oil, and vitamin E. If vitamin E is additionally included, the storage stability of the final manufactured product can be improved.

According to one embodiment of the present invention, the mixture of the calendula infuse oil and the mixed extract is added with beeswax, vitamin E, and at least one essential oil selected from the group consisting of chamomile essential oil, lavender French essential oil, and tea tree essential oil. It may further include a step (S14) of manufacturing a cosmetic in a balm or solid stick formulation.

The ingredients included in the cosmetic composition of the present invention are active ingredients and may include ingredients commonly used in cosmetic compositions in addition to the above-mentioned active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. Includes adjuvants and carriers.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing products. , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, etc., but is not limited thereto. More specifically, it can be manufactured as a balm formulation or a hard stick formulation.

When the cosmetic composition of the present invention is manufactured in a balm formulation or stick formulation, beeswax and essential oil can be used.

Hereinafter, the present invention will be described in more detail through preparation examples and examples. These manufacturing examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these manufacturing examples according to the gist of the present invention. .

Preparation Example 1: Moisturizing balm formulation

10 g of dried calendula and 100 g of green tea oil were mixed, placed in a washed container, and cooled in a cool place without direct sunlight for 30 days. The above containers were mixed by shaking them up and down 2 to 3 times every day. After 30 days, the solids were removed from each container to obtain calendula oil.

After mixing 60g each of dried astragalus, dried ash leaf, and dried sea buckthorn fruit, reflux-extracted and cold-soaked twice for 5 hours each with a 70% (v/v) ethanol aqueous solution, and then filtered using Whatman #4 filter paper. Filtered. Salts were removed from the filtered extract using an ultrafiltration membrane, and then concentrated under reduced pressure and freeze-dried below 50°C to obtain 5.8 g of powder.

Mix 17g of calendula oil prepared above, 1g of mixed extract, 4g of beeswax, 16 drops of chamomile essential oil, 34 drops of tea tree essential oil and 3g of vitamin E, stir slowly while heating to 33°C, place in a container and cool to form a balm formulation. A cosmetic composition was prepared.

Preparation Example 2: Moisturizing stick formulation

10 g of dried calendula and 100 g of green tea oil were mixed, placed in a washed container, and cooled in a cool place without direct sunlight for 30 days. The above containers were mixed by shaking them up and down 2 to 3 times every day. After 30 days, the solids were removed from each container to obtain calendula oil.

After mixing 60g each of dried astragalus, dried ash leaf, and dried sea buckthorn fruit, reflux-extracted and cold-soaked twice for 5 hours each with a 70% (v/v) ethanol aqueous solution, and then filtered using Whatman #4 filter paper. Filtered. Salts were removed from the filtered extract using an ultrafiltration membrane, and then concentrated under reduced pressure and freeze-dried below 50°C to obtain 5.8 g of powder.

Mix 17g of calendula oil prepared above, 1g of mixed extract, 16g of beeswax, 16 drops of chamomile essential oil, 34 drops of tea tree essential oil and 3g of vitamin E, heat to 33℃ and stir slowly, place in a container and cool to form a stick formulation. A cosmetic composition was prepared.

Comparative Manufacturing Example 1

Calendula oil was prepared using olive oil instead of green tea oil, and a balm-type cosmetic composition was prepared in the same manner as in Preparation Example 1, except that it did not contain mixed extracts of Astragalus, Cachyra sinensis, and sea buckthorn fruit. However, 18g of calendula oil was used.

Comparative Manufacturing Example 2

A balm-type cosmetic composition was prepared in the same manner as Preparation Example 1, except that green tea oil alone without calendula was used.

Comparative Manufacturing Example 3

A cosmetic composition in the form of a balm formulation was prepared in the same manner as Preparation Example 1, except that it did not contain mixed extracts of Astragalus, Astragalus, and Sea buckthorn fruit. However, 18g of calendula oil was used.

Experimental Example 1: Measurement of skin electrical conductivity

The clinical moisturizing effect and durability of the balm formulations prepared in Preparation Example 1 and Comparative Preparation Examples 1 to 3 were measured as follows. Through a survey, 50 healthy adult men and women in their 20s to 40s who felt that their skin was dry were randomly formed into groups of 10, and each group was given Preparation Examples 1 and 2 and Comparative Preparation Examples 1 to 3 twice a day for 4 weeks. It was applied to the face. The moisturizing effect was evaluated for 2 weeks from the start of the actual use test, and the improvement effect after the end was evaluated by quantifying the increase rate (%) in skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage + Khazaka, Germany). The experimental results are shown in Table 1 below.

division Before use After 2 weeks of use After 4 weeks of use Manufacturing Example 1 23.85 48.66 65.75 Production example 2 22.95 46.56 62.12 Comparative Manufacturing Example 1 23.12 36.19 45.52 Comparative Manufacturing Example 2 22.86 40.32 52.17 Comparative Manufacturing Example 3 23.26 39.11 48.39

Looking at Table 1, the moisturizing effect of the balm formulation (Preparation Example 1) and the stick formulation (Preparation Example 2) containing calendula oil and mixed extract of the present invention is greater than that of the balm formulation (Comparative Preparation Example 1, It was confirmed that the moisturizing effect was superior compared to Comparative Preparation Example 3) and was also superior compared to the balm formulation that did not use calendula oil (Comparative Preparation Example 2).

Experimental Example 2: Skin barrier improvement effect

The study was conducted on 25 healthy women indoors at 25°C, relative humidity of 45%, and no air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS), and Preparation Examples 1 and 2 and Comparative Preparation Examples 1 to 3 were applied to the upper arm and then treated with TEWAMETER TM210 (C+ Transepidermal water loss was measured using K eletronic GmhH. Germany). In other words, the skin barrier recovery rate (%) was confirmed by measuring the change in TEWL (Transepidermal Water Loss) value over time. The experimental results are shown in Table 2 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Manufacturing Example 1 60.86 79.37 88.69 90.18 Production example 2 57.38 74.58 85.46 87.68 Comparative Manufacturing Example 1 39.22 50.79 62.74 61.65 Comparative Manufacturing Example 2 43.53 58.24 71.23 76.14 Comparative Manufacturing Example 3 40.89 50.37 45.64 58.88

Looking at Table 2 above, the skin barrier recovery rate of the balm formulation (Preparation Example 1) and stick formulation (Preparation Example 2) containing calendula oil and mixed extract of the present invention is higher than that of the balm formulation (Comparative Preparation Example) not containing mixed extract. 1, Comparative Preparation Example 3) was found to be superior, and it was also confirmed to be superior compared to the balm formulation using green tea oil rather than calendula oil (Comparative Preparation Example 2).

Experimental Example 3: Safety test

A human skin patch test was conducted to determine whether Preparation Examples 1 and 2 irritated the skin. The test was conducted on 20 women in their 20s to 40s who had never shown hypersensitivity to skin irritation in the past and had no symptoms of skin disease or skin allergy. Comparative Preparation Example 3 did not contain mixed extracts of Astragalus, Soma and Sea buckthorn fruits. was used as a control example.

First, the test area was wiped with 70% ethanol and dried. The prepared test substance was dropped into a Finn chamber (100 The patch was applied for 24 hours, and the presence or absence of erythema and edema in the test area was observed using a magnifying glass (8MC-150, DAZOR) 1 hour and 24 hours after the patch was removed.

Skin reaction was determined according to the International Contact Dermatitis Research Group (ICDRG) regulations (Table 3), and the average skin reactivity was calculated using Equation 1 below and the results are shown in Table 4.

sign score Evaluation standard - 0 No response ± 0.5 faint or mild erythema + One Erythema, edema, and papules have clear boundaries but are mild. ++ 2 Marked erythema, papules, and vesicles +++ 3 Severe erythema, blisters, and crust formation

division 24 hours 48 hours Average skin response
(n=20) ± + ++ ± + ++ Manufacturing Example 1 - - - - - - 0.00 Production example 2 One - - - - - 0.14 Comparative Manufacturing Example 3 - - - - - - 0.00

As shown in Table 4, it can be seen that Preparation Examples 1 and 2 and Comparative Preparation Example 3 all have low average skin reactivity. At the same time, it can be confirmed that none of them cause skin irritation.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Preparing calendula infused oil by mixing dried calendula flowers and green tea oil at a weight ratio of 1:8 to 10 and maintaining it at room temperature for 20 to 40 days;
Preparing a mixed extract of Astragalus roots, leaves and sea buckthorn fruits by mixing dried Astragalus roots, leaves and sea buckthorn fruits at a weight ratio of 1:1:1 and then extracting them with an organic solvent; and
A method of producing a cosmetic composition for skin moisturizing, comprising mixing the calendula infuse oil and the mixed extract at a weight ratio of 10 to 100:1. The method of claim 1, wherein the green tea oil is prepared by drying and powdering green tea seeds and then using steam distillation. The method of claim 1, wherein the organic solvent is anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids, and these. A method for producing a cosmetic composition for moisturizing skin, selected from the group consisting of a mixture of. The method of claim 1, wherein the mixture of the calendula infused oil and the mixed extract includes beeswax, vitamin E, and at least one essential oil selected from the group consisting of chamomile essential oil, lavender French essential oil, and tea tree essential oil. , A method of producing a cosmetic composition for moisturizing skin, further comprising the step of preparing a cosmetic in a balm or solid stick formulation. The cosmetic composition for skin moisturizing according to claim 1, wherein the skin moisturizing cosmetic composition further comprises one or more ingredients selected from the group consisting of chamomile essential oil, lavender French essential oil, tea tree essential oil, and vitamin E. Manufacturing method.