KR101056756B1 - Cosmetic composition containing herb medicine extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing medicinal herb material extract is provided to ensure skin moisturizing, antioxidation, whitening, and skin elasticity without side effects. CONSTITUTION: A cosmetic composition for antioxidation, whitening, or moisturizing contains extract of Morus Alba, Acorus Gramineus, Ulmus Davidiana, Xanthium Strumarium, Cyperus Rotundus, Arisaema Amurense, Gastrodia Elata, Asparagus Cochinchinensis, and Asparagus Cochinchinensis. The medicinal herb extract is isolated using ethanol solution containing ethanol and water in a weight ratio of 30:70-70:30.

Description

Cosmetic composition containing herbal extracts {Cosmetic composition containing herb medicine extracts}

The present invention relates to an antioxidant, whitening or moisturizing cosmetic composition comprising as an active ingredient herbal extracts having useful effects such as antioxidant, whitening, moisturizing.

Aging of human skin is caused by endogenous aging caused by gradual deterioration of physiological function and activity of skin cells and decreased secretion of hormones that regulate metabolism and by external factors such as ultraviolet rays, free radicals or stress. It is divided into human aging, and as aging progresses, skin elasticity, wrinkle formation, and pigmentation appear.

The skin is composed of keratin, epidermis, dermis and subcutaneous tissue and functions to protect the body from physical and chemical stimuli from external factors or the environment. In particular, the skin plays a very important role in controlling the evaporation of the water, which constitutes about 70% of the body's constituents into the body.

Symptoms of skin aging include decreased elasticity, increased wrinkles, blemishes, and freckles. One of the causes of the skin aging is the chain breakage of collagen and elastin, which are elastic fibers, and abnormal crosslinking and chain breakage of hyaluronic acid. Known. Due to this phenomenon, the water retention capacity of the stratum corneum is reduced, and the elasticity of the skin is reduced by weakening the elastin fibrous tissue.

In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the superficial layer is about 10 to 30% in the keratinocyte layer. Moisture supplied from the dermis is mainly transferred to the top of the stratum corneum by passive diffusion and finally discharged to the outside. This is called trans epidermal water loss (TEWL), which is known to be maintained at an appropriate level by the sebaceous component and the epidermal lipid, which are the lipid membrane components of the keratinocyte layer. In addition, the keratinocyte layer is known to play a very important role in moisturizing the skin due to the presence of a substance having a hydrophilic water retention ability called a natural moisturizing factor (NMF). If the moisture of the keratinocyte layer is maintained at about 10-30%, the skin condition is good and the body protection function is normally exerted, but when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protection function is deteriorated. The phenomenon occurs. In addition, even healthy skin is deteriorated due to the lack of moisture caused by external factors, such as wind, cold weather, sunlight, face wash, shaving. Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin.

Various studies have been conducted as a method for preventing the loss of skin moisture and skin aging, and in particular, skin moisturizing-related materials have been developed and applied. In particular, as a main material having such an effect, water evaporation of a humectant and a ceramide, a fatty acid, a lipid complex, etc., having a property of absorbing moisture with a polyol (polyalcohol) such as glycerin, propylene glycol, butylene glycol, and polyethylene glycol Preventing closed moisturizers are known. Such skin moisturizing materials are known to have a disadvantage of severe stickiness or disturbing the stability of the formulation by disturbing the structure of the emulsion interface membrane in the emulsion formulation of the cosmetic.

Recently, natural materials obtained from animals, plants, and microorganisms as skin moisturizers have been developed to increase skin moisturizing power and elasticity, and development of antioxidant, whitening or moisturizing cosmetics having a whitening effect has been actively performed.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition using a safe, natural ingredients (medicinal herbs) that can improve skin aging phenomenon such as skin dryness, loss of elasticity, blemishes, freckles, spots, etc. It is.

In order to achieve the above technical problem, the present invention provides an antioxidant, whitening or moisturizing cosmetic composition comprising a specific herbal extract as an active ingredient, more preferably, upper leaf, Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonma Provides a cosmetic composition, in particular, an antioxidant, whitening or moisturizing cosmetic composition comprising all of the cheonmundong, Cheonoxidation and cheonhwabun extract as an active ingredient.

First, the present invention is a astronaut ( Gastrodia Elata ) provides an antioxidant cosmetic composition comprising an extract as an active ingredient, more preferably, the present invention, in addition to the cheonma extract, the upper leaf, Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonmun-dong, Cheon oxide It provides an antioxidant cosmetic composition, characterized in that it further comprises a cheonhwabun extract as an active ingredient.

In another aspect, the present invention Chunnam ( Arisaema Amurense ) provides a whitening cosmetic composition comprising an extract as an active ingredient, more preferably, in addition to the cheonnam extract extracts of the upper leaves, Seokchangpo , Yubaekpi , Changja , Hyangbuja , Cheonma, Cheonmun-dong, Cheonhyangchu and Cheonhwamin extract It provides a cosmetic composition for whitening, further comprising as an active ingredient.

In another aspect, the present invention provides a moisturizing cosmetic composition, characterized in that it comprises a hyangbuja extract, Cheonnam extract, Cheonma extract, Cheonnachu extract or a mixture thereof as an active ingredient, more preferably, upper leaf, Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, cheonma, cheonmun-dong, Cheonhyangchu and provides a moisturizing cosmetic composition comprising an extract as an active ingredient.

Morus Alba of the herbal medicine according to the present invention is a leaf of the mulberry, which is a deciduous broad-leaved arborescent of the mulberry family.

Among the herbal medicines according to the present invention, Seokchangpo ( Acorus Gramineus ) is an evergreen perennial herb of Chunnamseong writes the roots and stems as herbs.

Among the herbal medicines according to the present invention, Yubaekpi ( Ulmus Davidiana ) is the root bark of the elm, which is a deciduous broad-leaved arborescent of the elm family.

Changza ( Xanthium Strumarium ) of the herbal medicine according to the present invention is the fruit of the annual herbaceous larvae of the Asteraceae.

Among the herbal medicines according to the present invention, Cyperus Rotundus is the root of the perennial herb, perennial herb.

Cheonnamseong ( Arisaema) among herbal medicines according to the present invention Amurense ) is a perennial grass belonging to Chunnamseong Province.

Gastrodiaesis of the herbal medicine according to the present invention Elata ) is a tube of semiparasitic perennial grass belonging to the family Orchidaceae.

Asparagus Cochinchinensis of the herbal medicine according to the present invention is the root of perennial grass belonging to the family Liliaceae.

Among the herbal medicines according to the present invention, asparagus cochinchinensis is a tree of the family Rosaceae, also called tinnitus.

Asparagus Cochinchinensis of the herbal medicine according to the present invention is the root of Hanultari, a perennial vine plant belonging to the genus.

The present invention is based on the fact that certain herbal extracts have excellent antioxidant, whitening or moisturizing effect and are very useful as an active ingredient of cosmetic compositions, in particular upper leaf, Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmundong, Cheonoxidation Cosmetic compositions containing both weight and natural extracts are based on the surprising finding that the cosmetic effects such as antioxidant, whitening, and moisturizing effects are synergistically improved.

Therefore, the more preferred cosmetic composition having the antioxidant, whitening and moisturizing effect according to the present invention as an active ingredient extract of the upper leaf, Seokchangpo extract, baekbaekpi extract, Changja extract, Hyangbuja extract, Cheonnam extract, Cheonma extract, Cheonmundong extract, Cheonjuchu It may include a mixture of extracts and cheonhwabun extract, and more preferably may include extracts of 10 herbal medicine mixtures of lettuce, Seokchangpo, Yubaekpi, Changjaja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmun-dong, Cheonhyangchu and Cheonhwabun. . In view of extraction efficiency, manufacturing cost, etc., it is preferable to extract the herbal medicine mixture at once.

In the composite herbal medicine extract, it is preferable that the mixing ratio is maintained at a certain level so that the synergistic effects of the herbal medicines are maintained, and thus, the composition of the present invention has the weight of the total weight of the herbal medicines, leaf, changchang, baekbaekpi, Changja, Hyangbuja, Cheonnamseong, More preferably, the extract contains an herbal medicine mixture containing 1 to 20% by weight of cheonma, astronomical dong, cheon oxchu and cheonhwa flour.

Extraction solvent for preparing the herbal medicine extract according to the present invention is purified water; Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; Hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane; Or they may be a mixed solvent, in particular ethanol, purified water or ethanol aqueous solution is preferred, in this case the total weight of the total herbal medicine used for extraction is preferably 5 to 50% by weight relative to the weight of the extraction solvent.

In addition, in the cosmetic composition according to the present invention, the present invention by using the ethanol, purified water or ethanol aqueous solution as the extraction solvent, by adjusting the content ratio of ethanol and purified water, the efficacy of each of the cosmetic composition comprising the ten herbal extracts of the present invention It is based on the surprising fact that it can enhance specifically.

Specifically, in order to increase the antioxidant effect of the cosmetic composition comprising the 10 herbal medicine extracts of the present invention, it is preferable that the content ratio of ethanol and purified water is almost similar, and to increase the whitening effect, the higher the ethanol content is preferable, In order to increase the moisturizing effect, the higher the purified water content of the extraction solvent is preferable.

Therefore, more preferably, the present invention is a cosmetic composition containing the ten herbal extracts of the present invention as an active ingredient, wherein the extract is ethanol and water (ethanol: water) in a weight ratio of 30:70 to 70:30 Antioxidant cosmetic composition, characterized in that extracted using a mixed ethanol aqueous solution; Whitening cosmetic composition, characterized in that the extract is extracted with pure ethanol or ethanol aqueous solution of 80% by weight or more ethanol; And the extract provides a moisturizing cosmetic composition, characterized in that extracted with purified water or ethanol aqueous solution of 20% by weight or less.

In addition, the present invention is a method for producing a cosmetic composition containing the extract of the ten herbal medicines of the present invention as an active ingredient, the mixture of the ten herbal medicines is 30:70 to 70:30 of ethanol and water (ethanol: water) Method of increasing the antioxidant effect of the cosmetic composition, characterized in that the extraction using an aqueous solution of ethanol mixed in a weight ratio; Method of enhancing the whitening effect of the cosmetic composition, characterized in that the mixture of 10 herbal medicines extracted with pure ethanol or ethanol aqueous solution of 80% by weight or more; And it provides a method of increasing the moisturizing effect of the cosmetic composition, characterized in that the mixture of 10 herbal medicines extracted with purified water or ethanol aqueous solution of 20% by weight or less.

The extract used as an active ingredient in the cosmetic composition of the present invention is (i) a method of extracting the medicinal herb mixture by immersing it in an extraction solvent at 20 to 40 ℃, (ii) a herb mixture in the extract solvent at 60 to 90 ℃ to reflux Extraction method, (iii) a method of extracting the herbal medicine mixture in the extraction solvent at 20 to 70 ℃ by ultrasonic irradiation, (iv) the respective herbal medicines of each of the (i), (ii), (iii), It can be prepared by the method of separately extracting by the method of (iv) and mixing each extract according to a certain ratio, but is not limited thereto. However, the herbal extract is more preferably used as an ultrasonic extraction method to increase the extraction efficiency and improve the stability of the active ingredient.

Antioxidant, whitening or moisturizing cosmetic composition of the present invention comprises about 0.1 to 90% by weight, more preferably 1 to 10% by weight based on the total weight of the composition of the herbal extract as a main component.

On the other hand, the cosmetic composition prepared by containing the antioxidant, whitening or moisturizing herbal medicine extract may be prepared in a variety of formulations, such as cosmetics, lotions, creams, shampoos, specifically, emulsion, cream, paste It can be applied in various properties such as, it can be prepared by a conventional cosmetic preparation method. In particular, the aforementioned cosmetic composition is more preferably used as an antioxidant, whitening or moisturizing cosmetic.

The cosmetic composition of the present invention is a moisturizer, thickener, surfactant, emulsifier, preservative, antioxidant, alcohol, flavoring, pH adjusting agent, natural extract within the range that does not inhibit the effect of the present invention other than antioxidant, whitening or moisturizing herbal extracts And the like, as well as an antioxidant, whitening or moisturizing component of a chemical component for doubling the antioxidant, whitening or moisturizing effect.

The herbal extracts and cosmetic compositions of the present invention have excellent skin moisturizing effect, antioxidant effect, whitening effect and elasticity improvement effect and do not cause side effects such as skin irritation.

The specific herbal extracts for antioxidant, whitening or moisturizing presented in the present invention have beneficial cosmetic effects such as antioxidant, whitening or moisturizing, and especially upper leaf, stone spear, milk white skin, changja, hyangbuja having skin moisturizing and anti-aging effects. The herbal extract extracted from the combination of 10 kinds of herbal medicines, including Cheonnamseong, Cheonma, Cheonmun-dong, Cheonhyangchu and Cheonhwabun, showed synergistically improved skin moisturizing effect, antioxidant effect, whitening effect and elasticity improvement. In addition, the cosmetic composition according to the present invention is expected to be able to exert an excellent effect in the commercialization or commercialization of the product as a safe substance without skin irritation.

1 is a view showing the result of measuring the moisturizing power according to the extraction solvent ratio of the extract of antioxidant, whitening or moisturizing mixed herbal medicine according to the present invention.
Figure 2 is a diagram showing the results of measuring the moisturizing power of 10 individual herbal medicine extracts constituting the mixed herbal medicine extract and antioxidant herbal whitening or moisturizing herbal extracts according to the present invention.

Hereinafter, specific examples such as examples, formulation examples, etc. will be described in order to specifically describe the present invention. However, embodiments or formulations according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the examples described below. Examples and formulations according to the present invention are provided only to provide a more complete description of the present invention to those skilled in the art, and various equivalents and modifications that can be substituted for them at the time of the present application There may be examples.

<Compound Examples 1 to 5 and Single Examples 1 to 10>

In order to measure the antioxidant, whitening or moisturizing effect of the herbal extracts were prepared herbal extracts as described in Table 1 below. At this time, the unit of the component content is the weight ratio.

Compound Examples 1 to 5 shown in Table 1 were prepared by the ultrasonic extraction method as an example of extracting the herbal medicine extract according to the mixing ratio of the extraction solvent, the mixing ratio of purified water and ethanol, respectively 0: 100, 25:75, 50 An extraction solvent having a ratio of: 50, 75:25, and 100: 0 was used.

In addition, single Examples 1 to 10 are 10 kinds of medicinal herb extracts and composite extracts of the present invention, the upper leaf, Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmun-dong, Cheonjuchu and Cheonhwabun A single medicinal herb was extracted using an extraction solvent in a ratio of 50:50 of purified water and ethanol, and ultrasonic extraction was used in the same manner as in the composite examples.

Medicinal name Composite Example Single embodiment One 2 3 4 5 One 2 3 4 5 6 7 8 9 10 Upper lobe 1.0 1.0 1.0 1.0 1.0 10.0 - - - - - - - - - Seokchangpo 1.0 1.0 1.0 1.0 1.0 - 10.0 - - - - - - - - Milky skin 1.0 1.0 1.0 1.0 1.0 - - 10.0 - - - - - - - Changza 1.0 1.0 1.0 1.0 1.0 - - - 10.0 - - - - - - Hyangbuja 1.0 1.0 1.0 1.0 1.0 - - - - 10.0 - - - - - Cheonnam Province 1.0 1.0 1.0 1.0 1.0 - - - - - 10.0 - - - - Cheonma 1.0 1.0 1.0 1.0 1.0 - - - - - - 10.0 - - - Astronomical building 1.0 1.0 1.0 1.0 1.0 - - - - - - - 10.0 - - Cheon Oxidation 1.0 1.0 1.0 1.0 1.0 - - - - - - - - 10.0 - Natural flower 1.0 1.0 1.0 1.0 1.0 - - - - - - - - - 10.0 Purified water 0 25 50 75 100 50 50 50 50 50 50 50 50 50 50 ethanol 100 75 50 25 0 50 50 50 50 50 50 50 50 50 50

Experimental Example 1-1 Antioxidant Effect Evaluation (Free Radical Scavenging Test)

In order to investigate the antioxidant effect of the active ingredient of the cosmetic according to the present invention, a free radical scavenging activity test was conducted. The free radical scavenging test was performed by modifying the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303, 1993), and the stable free radical DPPH (1,1-diphenyl-2-picryl hydrazyl) , Sigma) reagent was used.

First, 1 mL of 0.2 mM DPPH solution is diluted to a suitable concentration of each sample obtained according to the above example in methanol, and 2 mL are mixed, and the absorbance is measured at 517 nm after being left at room temperature for 10 minutes. On the other hand, the control in the free radical scavenging test was set to obtain the color correction value for each example by adding methanol instead of the sample solution, and put methanol instead of the DPPH solution.

The free radical scavenging rate was calculated numerically using Equation 1 below, and the test was repeated three times, respectively, and the results are shown as mean ± standard deviation in Table 2 below. In Table 2 below, SC ₅₀ is a concentration of a sample required to achieve 50% free radical scavenging ratio, which is a value used in comparison with other component substances, and the smaller the value, the higher the scavenging ratio.

division SC ₅₀ (μg / mL) Composite Example 1 36.691 ± 0.557 Composite Example 2 26.318 ± 1.048 Composite Example 3 23.162 ± 0.144 Composite Example 4 37.608 ± 0.798 Composite Example 5 47.910 ± 1.663 Single Example 1 54.301 ± 0.802 Single Example 2 84.301 ± 0.505 Single Example 3 324.303 ± 2.058 Single Example 4 157.357 ± 1.496 Single Example 5 108.531 ± 2.117 Single Example 6 132.30 ± 2.165 Single Example 7 86.802 ± 0.991 Single Example 8 94.246 ± 2.829 Single Example 9 58.239 ± 1.001 Single Example 10 73.224 ± 0.714

As shown in Table 2, Single Example 1 (Upper Leaf), Single Example 2 (Sukchangpo), Single Example 7 (Cheonma), Single Example 8 (Astronomical Bronze), Single Example 9 (Chunoxidation Chu) And the superior free radical scavenging effect of the single Example 10 (cheonhwa), the free radical scavenging effect of the complex herbal extracts of Examples 1 to 5 of the complex herbal extracts of the present invention, the upper lobe of 10 kinds of single medicinal herbs constituting the herbal extracts , Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmun-dong, Cheonhyangchu and Cheonhwabun each showed a superior antioxidant activity compared to the single examples 1-10.

Experimental Example 1-2 Evaluation of Antioxidant Effect (Active Oxygen Scavenging Test)

In order to determine the antioxidant effect of the active ingredient of the cosmetic according to the present invention, the degree of active oxygen scavenging was evaluated by another evaluation method. The active oxygen scavenging test is a modification of the method of Noro et al. (Chem. Pharm. Bull., 31, 3984-3987, 1983). A change in absorbance due to oxidation of nitroblue tetrazolim (NBT) due to active oxygen was measured using an active oxygen generation system by an enzyme reaction.

2.4 mL of Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA, 0.1 mL of BSA, 0.1 mL of NBT, and 0.1 mL of sample were added thereto, mixed well, and allowed to stand at 25 ° C. for 10 minutes. After adding 0.1 mL of Xanthine oxidase and reacting at 25 ° C. for 20 minutes, 6 mM CuCl ₂ was added to stop the reaction. Absorbance was measured at an optical wavelength of 560 nm. On the other hand, the control group in the active oxygen scavenging experiment was set to obtain purified color for each of the Examples and Comparative Examples by adding purified water instead of the sample solution, and purified water instead of xanthine oxidase solution.

Reactive oxygen scavenging rate was calculated numerically by using Equation 2 below, and the test was repeated three times, respectively, and is represented as the average ± standard deviation in Table 3 below. In Table 3 below, IC ₅₀ is a concentration of a sample required to achieve 50% of an active oxygen scavenging ratio, and is a value used in comparison with other component substances. The smaller the value, the higher the scavenging ratio.

division IC ₅₀ (μg / mL) Composite Example 1 927.601 ± 5.337 Composite Example 2 117.310 ± 5.657 Composite Example 3 79.932 ± 6.705 Composite Example 4 455.932 ± 11.435 Composite Example 5 432.968 ± 8.838

As shown in Table 3, in the herbal extracts of the composite examples 1 to 5 according to the present invention, the composite example 3 using the mixed water and the ethanol mixed extract solvent in a ratio of 50:50 is the composite examples 1 to 2 and the composite example. It was confirmed that the active oxygen scavenging effect is very excellent compared to 4 to 5.

Experimental Example 2 Evaluation of Whitening Effect

In order to measure the whitening effect of the herbal extract for preventing skin aging presented in the present invention, the effect of inhibiting tyrosinase activity was measured. Tyrosinase is an enzyme that helps melanin production by promoting the oxidation process of a substance called tyrosine in vivo. The whitening effect experiment of the herbal extract for skin moisturization and skin aging using tyrosinase activity inhibitory effect was evaluated as a method of evaluating the degree of inhibition of melanin formation by inhibiting the function of the enzyme. The procedure for measuring tyrosinase activity inhibitory effect is as follows.

0.9 mL of the sample obtained through the composite and single examples, 1.0 mL of 0.1 M phosphate buffer (pH 6.8), and 1.0 mL of 1.5 mM L-tyrosine were added thereto, and then maintained at 37 ° C. for 10 minutes. After 0.1 mL of mushroom tyrosinase (1,500 units / mL) was added and reacted at 37 ° C. for 10 minutes, the absorbance was measured at 475 nm using a UV-vis spectrophotometer. Tyrosinase activity inhibition rate was calculated according to the following equation (3).

division IC ₅₀ (mg / mL) Composite Example 1 0.271 ± 0.857 Composite Example 2 0.842 ± 0.941 Composite Example 3 0.861 ± 1.012 Composite Example 4 1.523 ± 0.914 Composite Example 5 2.530 ± 0.868 Single Example 1 1.608 ± 0.925 Single Example 2 3.139 ± 1.115 Single Example 3 -(Low efficacy) Single Example 4 -(Low efficacy) Single Example 5 -(Low efficacy) Single Example 6 3.002 ± 1.004 Single Example 7 2.728 ± 0.902 Single Example 8 2.549 ± 0.842 Single Example 9 1.905 ± 0.776 Single Example 10 2.827 ± 0.681

As shown in Table 3 above, Single Example 1 (Upper Leaf), Single Example 2 (Sukchangpo), Single Example 6 (Tenamseong), Single Example 7 (Cheonma), Single Example 8 (Astronomical Building), Single Example 9 (Cheonxiu Chu) and Single Example 10 (Taiwanese) had a tyrosinase activity inhibitory effect, and the composite herbal extracts of the present invention were Examples 1 to 5, in particular, Examples 1 to 4 of Tyrosina. In the single Examples 1 to 10, which are the single extracts of each of the 10 single medicinal herbs constituting the medicinal herb extracts of the antagonist activity, the upper leaves, Seokchangpo, Yubaekpi, Changjaja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmun-dong, Cheonjuchu and Cheonhwabun It was confirmed that there is a relatively excellent effect.

In particular, the compound Example 1 using purified water and ethanol mixed extract solvent in the herbal extracts of the above Complex Examples 1 to 5 in a ratio of 0: 100 has a relatively superior effect of inhibiting tyrosinase activity compared to the Complex Examples 2 to 5 It was confirmed.

Experimental Example 3 Moisturizing Effect Clinical Trial Evaluation

As a clinical test for skin moisturizing effect using the herbal extracts obtained according to the complex examples 1 to 5 and single examples 1 to 10 of the present invention, a skin moisture meter (Corneometer, Courage + Khazaka, GmbH, Germany) was used. The skin moisturizing power was evaluated by measuring skin conductivity.

Moisturizing power measurement by skin moisture meter is a device that measures the moisture content of skin surface (e.g. stratum corneum) by capacitance measurement.It is not affected by the samples already applied locally when measuring skin moisture content. This has the advantage of maintaining a constant measuring level of 30 to 40 μm below the probe sensor contacts.

Skin Moisture Meter Under the constant temperature and humidity conditions maintained at room temperature of 20 ~ 25 ℃ and relative humidity of 40 ~ 55%, the skin moisture meter was used to measure the inside of the arm of 3 subjects. After the application of the μL was absorbed into the skin for 10 minutes after application of the sample, the initial value was measured and measured for each 10, 20, 30, 40, 50, 60 minutes elapsed time. In addition, one measurement site was measured five times each and displayed as an average value. Using the following equation 4, the skin conductivity increase rate (%) as a moisturizing power was calculated and shown as a numerical value. In addition, skin conductivity was measured and made into a basic value before sample application. The results obtained by measuring the skin conductivity are shown in FIGS. 1 and 2 and Table 5 below.

Moisturizing power (%) Minutes 0 min 10 minutes 20 minutes 30 minutes 40 minutes 50 minutes 60 minutes Purified water 0 89.3 86.0 64.5 48.9 19.0 4.3 Composite Example 1 0 123.6 116.3 111.3 86.3 58.4 36.2 Composite Example 2 0 139.3 127.3 120.6 106.4 73.7 50.1 Composite Example 3 0 172.5 163.8 149.6 141.6 115.6 97.1 Composite Example 4 0 191.2 173.8 161.5 150.3 124.6 104.7 Composite Example 5 0 200.1 178.0 163.7 154.7 114.5 94.0 Single Example 1 0 135.2 122.9 111.8 106.2 88.5 73.2 Single Example 2 0 121.3 117.8 110.0 95.2 84.0 70.6 Single Example 3 0 156.3 142.0 130.6 121.7 101.7 82.5 Single Example 4 0 148.3 136.3 125.4 118.9 99.1 81.5 Single Example 5 0 145.2 133.4 121.4 112.9 94.8 86.3 Single Example 6 0 133.2 123.5 112.7 105.8 87.8 73.6 Single Example 7 0 140.0 127.3 118.5 110.6 91.4 76.7 Single Example 8 0 132.4 126.0 116.0 95.6 77.0 52.4 Single Example 9 0 150.6 144.4 134.8 123.2 103.2 85.1 Single Example 10 0 133.6 121.5 105.4 89.5 80.5 61.5

As shown in Table 5 and Figure 2, the single herbal extracts according to the present invention was relatively excellent moisturizing power compared to the purified water control group, in particular, the composite herbal extracts of the present invention (in particular, of the composite examples 3 to 5 Extract) skin moisturizing effect of 10 single medicinal herbs constituting the medicinal herb extract, upper leaf, Seokchangpo, baekbaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmun-dong, Cheonhyangchu and Cheonhwa single single extract 1 to 1 each It was confirmed that it is relatively superior to 10.

In addition, the skin moisturizing increase rate according to the time of the composite herbal extracts of Examples 1 to 5 according to the present invention was excellent compared to the purified water as a control, in particular, the mixing ratio of purified water in the extraction solvent was 50% or more of the complexed Examples 3 to 5 skin It was confirmed that the moisturizing capacity increase rate is relatively superior to the composite examples 1 to 2.

<Formulation Example 1 and Comparative Formulation Example 1>

Component composition of the moisture skin containing the active ingredient material of the cosmetic composition according to Example 1 was prepared as shown in Table 6. At this time, the unit of component content is weight%. On the other hand, of the components distinguished by each component number in Table 6 below, components 2 and 3 are first stirred and dispersed in component 1 (purified water), and then components 4 to 6 are added, and components 7 to 9 are added and mixed in a manner. Prepared. As a comparative formulation example, the remainder of the component constitution except for component 9 and the preparation method thereof were produced in the same manner.

number ingredient Formulation Example 1 Comparative Formulation Example 1 One Purified water Balance Balance 2 Butylene Glycol 2.0 2.0 3 glycerin 1.0 1.0 4 Fiji-1500 0.5 0.5 5 ethanol 4.0 4.0 6 Polysorbate 80 0.2 0.2 7 incense 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Active ingredient of the cosmetic according to Example 1 2.0 -

<Formulation Example 2 and Comparative Formulation Example 2>

Component composition of the lotion containing the active ingredient material of the cosmetic composition according to Example 1 was prepared by configuring as shown in Table 7. At this time, the unit of component content is weight%. On the other hand, among the components identified by each component number in Table 7 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 8 to 11 were dissolved and dispersed in component 12, and emulsified in those heated to 70 ° C. . Thereafter, the emulsified product was neutralized with component 13, cooled to a temperature of 56 ° C, and then component 14 was added thereto, stirred, and cooled to room temperature.

number ingredient Formulation Example 2 Comparative Formulation Example 2 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate / Pig-100stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 4.0 4.0 7 Apricot Kernel Oil 4.0 4.0 8 glycerin 5.0 5.0 9 Carbomer 0.1 0.1 10 Xanthan Gum 0.03 0.03 11 antiseptic a very small amount a very small amount 12 Purified water Balance Balance 13 Arginine 0.1 0.1 14 Active ingredient of the cosmetic according to Example 1 5.0 -

<Formulation Example 3 and Comparative Formulation Example 3>

Component composition of the nutrient cream containing the active ingredient material of the cosmetic composition according to Example 1 was prepared by configuring as shown in Table 8. At this time, the unit of component content is weight%. On the other hand, among the components identified by the component numbers in Table 8 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 9 to 12 were dissolved and dispersed in component 13, and emulsified in those heated to 70 ° C. . Thereafter, the emulsified product was cooled to a temperature of 56 ° C., and then component 14 was added thereto, stirred, and cooled to room temperature.

number ingredient Formulation Example 3 Comparative Formulation Example 3 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate / Pig-100stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 8.0 4.0 7 Apricot Kernel Oil 4.0 4.0 8 Dimethicone 2.0 2.0 9 glycerin 5.0 5.0 10 Magnesium aluminum silicate 0.4 0.4 11 Xanthan Gum 0.03 0.03 12 antiseptic a very small amount a very small amount 13 Purified water Balance Balance 14 Active ingredient of the cosmetic according to Example 1 10.0 -

Experimental Example 4 Skin Drying Improvement and Skin Elasticity Improvement Sensory Test

In order to evaluate the skin dry improvement effect (reduce pull and roughness, increase glossiness) and skin elasticity improvement effect of the skin moisturizing cosmetic composition according to the present invention, using the moisture skin prepared in Formulation Example 1 and Comparative Formulation Example 1 The sensory test was carried out. Specifically, in order to measure the drying improvement effect and elasticity improvement effect of the skin when each of Formulation Example 1 and Comparative Formulation Example were applied to the skin, 10 women over 20 years of age were used continuously at least once a day for 2 weeks. .

In the sensory test, the drying improvement effect and the elasticity improvement effect of the skin were relatively evaluated on the drying improvement effect and the elasticity improvement effect of the moisture skin of Formulation Example 1 based on the moisture skin of Comparative Formulation Example 1. The evaluation was performed based on the five-point method of very good (5 points), excellent (4 points), moderate (3 points), bad (2 points), very bad (1 point), and the results are shown in Table 9 below. Indicated. In Table 9, the skin irritation indicates the degree of no skin irritation.

number Skin Drying Improvement Skin elasticity improvement effect One 5 4 2 4 5 3 5 5 4 5 4 5 5 5 6 4 5 7 5 4 8 4 5 9 5 5 10 4 5 Average 4.6 4.7

As shown in Table 9, Formulation Example 1 containing an antioxidant, whitening or moisturizing herbal medicine extract according to the present invention is relatively improved compared to Comparative Formulation Example 1, the drying effect of the skin to an evaluation score of 4.6 points The skin elasticity improvement effect was 4.7 points, which was very good.

Experimental Example 5 Skin Safety Experiment

To know whether the occurrence of irritation or allergic reaction through the observation of skin safety, namely the skin reaction of the composite examples 1 to 5 of the complex herbal extract of the present invention and the formulation examples 1 to 3 of the cosmetic composition comprising the herbal extracts For this purpose, the human patch test was carried out according to the Korea Food and Drug Administration standard skin safety test method.

Thirty healthy men and women aged twenty to fifty were selected as subjects. The patch test was carried out on the inner side of the subject's upper arm and the patch was removed after 48 hours. The first reading was performed after about 60 minutes of stability, and the second reading was taken after 96 hours of patch application. Criteria are as shown in Table 10 below.

reaction weight Criteria of Judgment - 0 No response ± 0.5 Faint erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and blisters +++ 3 Cannon

The skin readiness of the sample was calculated using Equation 5 below after 48 hours and 96 hours after patch application.

The skin irritation degree of the sample showing the positive response calculated based on the test result of the patch test is shown in Table 11 below.

sample 48 hours 96 hours Responsiveness (%) + ++ + ++ 48 hours 96 hours Average Composite Example 1 - - 2 - 0.00 1.11 0.56 Composite Example 2 - - One - 0.00 0.56 0.28 Composite Example 3 - - One - 0.00 0.56 0.28 Composite Example 4 - - One - 0.00 0.56 0.28 Composite Example 5 - - - - 0.00 0.00 0.00 Formulation Example 1 - - One - 0.00 0.56 0.28 Formulation Example 2 - - - - 0.00 0.00 0.00 Formulation Example 3 - - - - 0.00 0.00 0.00

As shown in Table 11, the skin reactivity test according to Table 12 in the human patch test for the composite examples 1 to 5 of the complex herbal extract of the present invention and the formulation examples 1 to 3 of the cosmetic composition comprising the herbal extracts According to the criteria, it can be used for sensitive skin without irritation. Therefore, the herbal extract of the present invention and the cosmetic composition including the same have been shown as a safe ingredient having no skin side effects.

Average responsiveness of the skin Criteria 0.0 to 0.9 No stimulation 1.0 to 2.9 Light stimulation 3.0 to 4.9 Heavy stimulation 5.0 or more Strong stimulation

Claims (11)

Antioxidant, whitening or moisturizing cosmetic composition, characterized in that it comprises an extract of the upper leaf, Seokchangpo, Yubaekpi, Changja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmun-dong, Cheonhyangchu and Cheonhwabun as active ingredients. delete delete The cosmetic composition according to claim 1, wherein the extract is extracted using an ethanol aqueous solution in which ethanol and water (ethanol: water) are mixed in a weight ratio of 30:70 to 70:30. delete delete The cosmetic composition according to claim 1, wherein the extract is extracted with pure ethanol or an ethanol aqueous solution having an ethanol content of 80% by weight or more. delete delete The cosmetic composition according to claim 1, wherein the extract is extracted with an aqueous ethanol solution having a purified water or ethanol content of 20% by weight or less. The method according to any one of claims 1, 4, 7, and 10, wherein the composition is based on the total weight of the medicinal herb, lettuce, Seokchangpo, Yubaekpi, Changjaja, Hyangbuja, Cheonnamseong, Cheonma, Cheonmundong, Cheonsanchu And extracts of herbal medicine mixtures containing 1 to 20% by weight of cheonhwahwa.

Images