KR20090092095A - Anti-skin aging or anti-wrinkle cosmetic composition comprising specific herbal extracts - Google Patents

Abstract

A cosmetic composition for anti-aging or anti-wrinkle, which contains Scutellaria baicalensis Georgi, acacia, and strobilacea platycarya fruit extracts is provided to have excellent antiaging effect and anti-wrinkle without side effects. A composition for anti-aging or anti-wrinkle comprises a Scutellaria baicalensis Georgi extract and acasia extract. The composition further comprises Platycarya strobilacea extract. A method for producing mixed plant extracts comprises: a step of naturally drying root of Scutellaria baicalensis Georgi, leaves of acasia and fruit of Platycarya strobilacea; a step of adding one to ten times of ethanol based on weight of dried plant; a step of filtering the extracts; and a step of decompressing.

Description

Anti-aging or anti-wrinkle cosmetic composition comprising plant extracts

The present invention relates to a cosmetic composition for preventing skin aging or improving wrinkles, and in particular, a cosmetic composition further comprising a golden extract and acacia extract, or oyster barberry extract, having micro-inflammatory inhibitory effect, antioxidant activity and anti-wrinkle action. It relates to a composition.

Aging is a process in which the structure and function of the body gradually decreases with age, and as a result, the sensitivity to disease and death increases rapidly. These changes lose the ability to withstand the stresses caused by factors both inside and outside of life, thus losing homeostasis within the cell. Although research on the cause and occurrence of aging has been conducted for centuries, it is a reality that it is impossible to determine 100% of the decisive causes because it is a complex phenomenon in the human body, and various skin aging causes are known.

Among the aging hypotheses, the free radical hypothesis or oxidative damage hypothesis is best described as the aging phenomenon caused by oxidative stress, and it has been well supported and explains the aging phenomenon (Harman 1978 AGE 1, 143-150, 1978; Yu et al. 1996, Free Radic. Biol. Med. 21, 651-668).

In particular, the molecular inflammation hypothesis that the micro-inflammatory response plays an important role in the control of aging by causing the generation of reactive oxygen species or active nitrogen species generated during the aging process (Chung et al. 2001, Ann NY Acad.Sci. 928, 327-335).

The skin is located on the outermost surface of the body, and can be divided into three layers of epidermis, dermis and subcutaneous tissue from the upper layer, each layer having unique features and functions. Skin is the largest and most changeable organ and plays an important role in maintaining homeostasis of the human body because it plays an important role in protecting the body's internal environment from external stimuli. When each layer of skin has a normal structure and function, it can maintain the best skin condition in terms of physiology or beauty.

The aging process of the skin can be divided according to the causes of aging. In other words, it can be divided into aging (intrinsic aging), which is caused by aging, and extrinsic aging caused by external factors such as ultraviolet rays (Oikarinen, 1990, Photodermatol.Photoimmuno.Photomed. 7, 3-4).

Endogenous aging changes with age and decreases in cell number and skin thickness due to decreased function and skin atrophy. In other words, it is characterized by functional change rather than visual morphological change.

Exogenous aging is a change caused mainly by ultraviolet rays, and shows changes in thick skin thickness and deformed cell changes. In other words, exogenous aging causes structural and physiological changes, which adversely affects skin beauty and adversely affects skin health and beauty. Exogenous aging is a result of a combination of endogenous aging and environmental aging due to ultraviolet rays (Arch. Dermatol. 130: 28; 1994; Phtodermatol. Photoimmunol. Photomed. 7: 3; 1990, Dermatol. 21 : 610; 1989, K. Am. Acad. Dermatol. 21: 614; 1989, J. Cutan. Aging. Cosmet.Drmatol. 1: 5; 1988).

Factors that have a significant effect on the skin aging caused by UV light have been known to be caused by the action of free radicals and free radicals that are abnormally increased by UV light and thereby increase the activity of matrix metalloproteinases (MMPs).

In particular, 90% of the dermal layer is composed of collagen (collagen), and the reduction of collagen is closely related to skin aging, so the increase of matrix metalloproteinases (MMPs) activity is an important factor in skin aging and wrinkle formation. Matrix metalloproteinases (MMPs) are secreted from the fibroblasts and keratinocytes of the skin, breaking down most of the components that make up the extracellular matrix and basement membrane, destroying connective tissue that maintains skin elasticity, resulting in wrinkles and elasticity. Lowering and causing sagging skin (J. Biol. Chem, 277: 45154; 2002).

Fisher, etc., increases the activity of matrix metalloproteinases (MMPs) in the skin even during a single UV irradiation, and significantly breaks down collagen in the skin. Has been reported to play a very important role (Photochem. Photobiol. 69: 154; 1999, J. Photochem. Photobio. B. 63:41; 2001).

In the early stages of this chain reaction, it has been reported that microinflammatory reactions related to inflammation (molecular inflammation) play an important role as a major source of free radicals. In these inflammatory reactions, enzymes that produce not only free radicals but also active species such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), Xanthin Oxidase (XOD), and nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) Have been reported to play an important role.

These molecular inflammatory reactions are closely related to reactive oxygen species and reactive nitrogen species, which lead to the activation of NF-κB. NF-κB is the key to the central position in the inflammatory process. Various oxidative stresses in the skin aging process cause damage to the tissues, bring about inflammatory cytokine induction and activation of inflammatory cells, and continuously amplify the inflammatory response to accelerate the aging of the skin.

And, free radical reaction caused by active oxygen such as superoxide anion radical, hydroxy radical, singlet oxygen and hydrogen peroxide (H ₂ O ₂ ) derived from inflammatory reaction Destroys the major macromolecules of cells, including lipids, which are highly reactive and cause imbalances in cell constituents, cell membranes, and antioxidants, resulting in peroxidation, inactivation of defense mechanisms in vivo, and oxidation and degeneration of proteins. Accelerates skin aging, which impairs the function of, and is eventually characterized by decreased elasticity, wrinkle wrinkles and freckles.

Inflammation-related indicators have recently been reported to change with aging as aging progresses, suggesting that molecular inflammatory processes are important in the aging process.

To date, skin anti-aging materials have been concentrated in three categories: control of differentiation and regeneration of skin cells, control of extracellular matrix components, and ROS scavenging.

The inventors have recognized the importance of the development of inhibitors of aging through the regulation of NF-κB, which plays a key role in molecular inflammatory reactions. As a result of the study of the control, it was found that the mixed plant extract of the present invention, the collagenase inhibitory effect, the anti-aging effect through the control of NF-κB, and the anti-oxidant effect, etc., were effective in preventing skin aging and skin wrinkle improvement. This invention was completed.

An object of the present invention is to provide a cosmetic composition containing a mixed plant extract excellent in anti-aging and wrinkle improvement effect as an active ingredient.

The present invention relates to a cosmetic composition for preventing skin aging and improving skin wrinkles containing golden extract and acacia extract, or additionally, oyster bark fruit extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

Golden (Scutellaria baicalensis Georgi) of the raw material of the golden extract of the composition according to the present invention is a perennial herb of the dicotyledon plant moss nectar and is called soseuneun grass and the root is called golden.

The main ingredients are Baicalin, Baicalein, Ugonoside and Neoobaicalein in the root. Others include beta-sitosterol and amino acids. It is known to have stimulating, antiallergic, anti-inflammatory and sedative effects.

Acacia, a component of the composition according to the invention, is a genus of legumes and shrubs. Acacia is of great economic importance and provides a source of tannin, rubber, wood, fuel and feed. Tannins, primarily isolated from bark, are widely used to tan leather and fur. Some acacia bark is also used to add flavor to local spirits. Some native species, such as A. sinuata, also produce saponins, one of a variety of plant glucosides that, when mixed and stirred with water, form soap bubbles. Saponins are used in detergents, blowing agents and emulsifiers. Flowers of some acacia species are fragrant, used to make fragrances, and are known to have a soothing effect on the skin.

To date, about 330 compounds have been isolated from various acacia species. Flavonoids are the major class of compounds isolated from acacia, with about 180 different flavonoids identified, about 110 of which are flavans. Terpenoids are the second largest class of compounds isolated from species of the acacia genus, with 48 compounds identified. Other classes of compounds isolated from acacia include alkaloids (28), amino acids / peptides (20), tannins (16), carbohydrates (15), oxygen heterocyclic compounds (15) and aliphatic compounds (10) (Buckingham, The Combined Chemical Dictionary, Chapman & Hall CRC, version 5: 2 Dec. 2001).

One component of the composition of the present invention, Platycarya strobilacea, also called koji tree, is distributed in Korea, Japan, Taiwan, and China. It is known that oyster bark has eugloons and the fruit has tannins. It is called Hyangsu fruit in oriental medicine, and it has been used for the treatment of myalgia, abdominal pain, toothache, eczema swelling, etc. because it has the effects of analgesic, swelling and swelling. The leaves have a strong insecticidal and bactericidal action and are used for the treatment of acute purulent skin and swelling of the head. Reportedly, oyster bark extract has a physiological activity that inhibits the growth of harmful intestinal bacteria (Korean Patent Registration No. 10-0453290), an ellagic acid derivative and diaryl hepta as an active ingredient isolated from the oyster tree Dioid heptanoids are known (Phytochemistry. 47 (5): 851-854; 1998).

The plant extract contained in the cosmetic composition of the present invention may be prepared as follows.

The extract contained in the cosmetic composition of the present invention is washed with golden root, acacia heart and leaves, or oyster tree fruit with water, and then dried in a conventional drying method, preferably natural, and the dried product in fine or powder form Then, the active ingredient is extracted by a conventional extraction method.

Golden, acacia, oyster tree extract of the present invention is about 1 to 20 times the volume (ml) of water, alcohol, ketones, esters of finely divided or powdered golden, acacia or oyster tree dry weight (g) as described above. , A solvent selected from the group consisting of halogenated hydrocarbons and mixtures thereof, once by an extraction method such as hot water extraction, cold extraction, ultrasonic extraction or reflux cooling extraction for about 5 to 48 hours at an extraction temperature of room temperature to 100 ℃ After repeated extraction 5 times, the extract may be prepared by filtration, concentrated under reduced pressure or lyophilized. The solvent content in the final extract thus prepared is preferably less than 1%, more preferably less than 0.1%.

Preferably, the extractant is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol, butanol, 1,3-butylene glycol, etc.), lower ketone having 1 to 4 carbon atoms (e.g. Acetone, etc.), lower esters of 1-4 carbon atoms (e.g. ethyl acetate, butyl acetate, etc.), chloroform and mixtures thereof, more preferably hydrous lower alcohols having 1-4 carbon atoms, most Preferably ethanol can be used. However, the extractant is only a preferred example, and extracts having substantially the same effect can be obtained using other extractants.

In addition, the extract of the present invention includes not only the extract by the extraction solvent, but also an extract that has undergone a conventional purification process.

Obtained by various additional purification methods, such as separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention. Extracts of the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying. In the chromatography, preferably, a mixed solvent or lower ester, lower alcohol and water in which the halogenated hydrocarbon solvent and the lower alcohol are mixed in a ratio of 2: 1 to 100: 1, preferably 5: 1 to 20: 1. Mixed solvents mixed in a ratio of 1: 0 to 6: 4: 2 can be used as the eluent.

The anti-aging or anti-wrinkle cosmetic composition of the present invention may include a golden extract and acacia extract, or may further include an oyster tree fruit extract.

In the anti-aging or anti-wrinkle cosmetic composition of the present invention, the golden extract and the acacia extract are preferably present in a weight ratio of 0.1 to 10: 0.1 to 10.

 In addition, when further comprising the oyster tree fruit extract, the weight ratio of the golden extract and acacia extract and the oyster tree fruit extract is preferably 0.1 to 10: 0.1 to 10: 0.1 to 10, respectively.

The mixed plant extract can be used to prepare an extract from each plant and mix in the weight ratio.

The mixed plant extract is characterized in that it contains 0.001 to 90% by weight, preferably 0.5 to 30% by weight relative to the total weight of the composition.

Anti-aging cosmetic composition according to the present invention can be used in external medicines, cosmetics, etc., can be prepared in any formulation commonly prepared in the art. For example, ointments, tinctures, soaps and conventional cosmetics such as creams, massage creams, cold creams, lotions, milk creams, nutrition creams, moisturizing creams, essences, packs, foundations, powders, face washes, face washes, It can be formulated into a variety of facial foam, body shampoo, body gel, body cream, hand cream and the like.

In formulating them, cosmetics are prepared by using auxiliaries and excipients commonly used in the manufacture of external medicines, soap bases and cosmetics, such as emulsifiers, preservatives, lubricants, diluents, thickeners, humectants, pigments, preservatives, and fragrances. It can manufacture and use according to the method normally used for manufacture.

Moisturizers include one or two of the existing moisturizing ingredients such as ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, sorbitol, and collagen. The above can be mixed and used.

As the emulsifier, cyclomethicone, polymethyl methacrylate, or hydrogenated castor oil may be used.

As the thickener, methylcellulose, carboxymethylcellulose, carboxymethylhydroxyguanine, hydroxymethylcellulose, hydroxyethylcellulose, carboxyvinyl polymer, polyquaternium, cetearyl alcohol, stearic acid, carrageenan and the like can be used.

Preservatives can be imidazolidinyl urea, methyl paraben, propylparaben and the like.

Gold, acacia, oyster bark fruit extract of the present invention shows an excellent anti-aging effect by the synergistic effect of each component, even if a much smaller amount than the amount used for antioxidant purposes, and also shows excellent efficacy in improving skin wrinkles, It can be usefully used for cosmetics for the purpose of anti-aging or wrinkle improvement.

In addition, all of the anti-aging or anti-wrinkle plant extracts used in the composition according to the present invention have the advantage that their stability is already established and can be used without side effects.

Figure 1 shows the DPPH radical scavenging ability (%) of Comparative Examples 1 to 6 and Examples 4 to 6 extract.

Figure 2 shows the inhibitory effect of activation on NF-κB extracts of Examples 4-6.

Figure 3 shows the expression (%) MMP-1 expression of Comparative Examples 7 to 9 and Examples 7 to 9 extract.

Figure 4 shows the measurement of the improvement effect on the eye wrinkles of the nutrition cream of Comparative Example 10 and the nutrition cream prepared in Examples 10 to 12.

The present invention will be described in more detail based on the following Examples and Experimental Examples, but the present invention is not limited thereto.

Example  One: Preparation of Golden Extract

The golden roots were washed thoroughly with purified water, then completely dried and ground. 1-10 times of ethanol was added to the dry weight of gold, followed by extraction at 15-45 ° C. for 7 days in an extractor with a cooling capacitor. Thereafter, the filtrate was filtered through a 300 mesh filter cloth, filtered using Whatman No. 2 filter paper, and concentrated under reduced pressure at 45-50 ° C. using a distillation apparatus equipped with a cooling capacitor to prepare a golden extract.

Example  2:  Preparation of Acacia Extract

Acacia heart and leaves were washed thoroughly with purified water, and then completely dried and ground. To this was added 1-10 times ethanol to the dry weight of the acacia, and then extracted for 7 days at 15-45 ℃ for 3 days in the extractor attached to the cooling capacitor. Thereafter, the resultant was filtered through a 300 mesh filter cloth, filtered through one of Whatman Nos. 2 to 5 filter papers, and then concentrated under reduced pressure at 45-50 ° C. using a distillation apparatus equipped with a cooling capacitor to prepare an acacia extract.

Example  3: Oyster tree  Preparation of Fruit Extract

The oyster fruit was washed thoroughly with purified water, then completely dried and ground. 1-10 times of ethanol was added to the dry weight of the oyster fruit, and then extracted in a extractor with a cooling capacitor for 7 days at 15-45 ° C. for 3 days. Thereafter, the filtrate was filtered through a 300 mesh filter cloth, filtered using Whatman No. 2 filter paper, and concentrated under reduced pressure at 45-50 ° C. using a distillation apparatus equipped with a cooling capacitor to prepare an extract of oyster tree.

Example  4 to 9 : Golden extract and acacia extract, or additionally Oyster tree  Preparation of Compositions Containing Fruit Extracts

Examples 4 to 9 were prepared by mixing the golden extract prepared in Example 1 and the acacia extract prepared in Example 2 to be contained as shown in Table 1 below with respect to the total volume of the blend (ml).

TABLE 1 Amount of each plant extract in the composition (unit: µg / ml)

Example Golden extract Acacia Extract Oyster tree  Lychee extract 4 4 16 5 10 10 6 16 4 7 8 2 5 8 8 2 10 9 8 2 20

Example  10 to 12

4 parts by weight of cytosterol, 3 parts by weight of polyglycerol 2-oleate, 0.7 parts by weight of ceramide, 2 parts by weight of Ceteareth-4, and 3 parts by weight of cholesterol were homogenized.

The golden extract prepared in Example 1, the acacia extract prepared in Example 2 and the oyster barberry fruit extract prepared in Example 3 are mixed to be contained in the ratio as shown in Table 2 to the total volume (ml) of the formulation The composition according to the invention was prepared.

To the homogenized mixture, 3 parts by weight of powder of the composition, 0.4 parts by weight of dicetylphosphate, 5.0 parts by weight of concentrated glycerin, 22 parts by weight of sunflower oil, 0.5 parts by weight of carboxyvinyl polymer and a preservative were added to dissolve the solution, followed by purified water. After adding 56.4 parts by weight, emulsified in a homogenizer, an appropriate amount of perfume was added and stirred and mixed again to prepare Examples 10 to 12.

TABLE 2 Amount of each plant extract in the composition (%: w / v)

Example Golden extract Acacia Extract Oyster Tree Fruit  extract 10 1.60 0.40 11 2.00 12 0.80 0.20 1.00

The golden extract prepared in Example 1, the acacia extract prepared in Example 2 and the oyster barberry extract prepared in Example 3 were contained as shown in Table 3 with respect to the total volume (ml) of the extract. 9 was prepared.

TABLE 3 Amount of each plant extract in the composition (unit: µg / ml)

Comparative example Golden extract Acacia Extract Oyster Tree Fruit  extract One 10 2 20 3 50 4 10 5 20 6 50 7 10 8 20 9 50

Comparative example  10

Nutritional creams were prepared in the same manner as in Examples 10 to 12, except that the formulations of the present invention were not contained in Comparative Example 10.

Experimental Example

[ Experimental Example  1: antioxidant activity assay test]

The ability to reduce the DPPH radicals of the sample was assayed by measuring the antioxidant activity of the golden extract and acacia extract combinations obtained in Examples 4-6 above.

After dissolving a certain amount of DPPH (0.0039mg) with ethanol (50ml) to make a 0.2mM DPPH solution, and taking a predetermined amount of the sample dissolved in 70% ethanol to make a sample solution of 3.0mg / ml concentration, in Examples 4 to 6 0.5 ml of 0.2 mM DPPH solution was added to 1 ml of the compound and Comparative Examples 1 to 6, respectively, and the mixture was allowed to stand for 10 minutes and then absorbance was measured at 525 nm.

Blank was used by adding 1 ml of sample solution for each concentration to 0.5 ml of ethanol, and negative control group was used by adding 1 ml of 70% ethanol and 0.5 ml of 0.2 mM DPPH solution to the test tube.

DPPH radical scavenging ability of each sample was obtained by the following formula, and the results are shown in Table 7 and FIG. 1.

DPPH radical scavenging activity = [1- (sample absorbance-blank absorbance) / negative control absorbance] X 100

Table 4 Antioxidant Activity of Golden Extract and Acacia Extract Combination

DPPH Radical Scavenging power  (%) Comparative example  One 32.82 Comparative example  2 71.32 Comparative example  3 92.72 Comparative example  4 13.2 Comparative example  5 22.5 Comparative example  6 35.5 Example  4 70.78 Example  5 92.28 Example  6 99.24

From the results described in Table 4 and FIG. 1, gold and acacia extracts can be expected to have excellent DPPH radical scavenging ability and an anti-aging effect due to antioxidant activity as compared to the comparative example.

[ Experimental Example  2 : NF Activation Inhibition Assay for κB]

Human dermal fibroblasts (DPK-SKDF-H; Dominion Pharmakine (Bizkaia, Spain)) with 10% FBS, L-glutamine (2.0 mM), and penicillin / streptomycin (100 U / ml, 100 μg / ml) In a 75 cm ² plastic flask containing 15 ml of DMEM medium was incubated at 37 ℃, 5% CO ₂ conditions.

Cultured fibroblasts were aliquoted to 1.3 X 10 ⁵ cells / well in 6-well plates and incubated for 12 hours under the same conditions.

The medium of the cells was changed and 100 μl of each of Examples 4 to 7 was added thereto and incubated for 12 hours.

Iron sulfide (FeSO ₄ ) and ascorbic acid were treated in 6-well plates at concentrations of 0.5 μM and 0.25 mM, respectively, to induce the generation of harmful oxygen, causing oxidative stress.

After 24 hours of incubation, the experiment was conducted according to a manual provided using Chemicon International's NF- κ B assay kit (NF- κ B p50 / 65 Transcription Factor Assay Colorimetric).

TABLE 5 Degree of inhibition of NF- κ B activation of golden extract and acacia extract combination

NF degree of -κB activation ( OD _{450 nm} Of mg  protein) Untreated group 0.23 Control 1.82 Example  4 1.26 Example  5 0.84 Example  6 0.73

Combinations of gold extract and acacia extract, such as the Figure 2 and in Table 5 was found in the activation of NF- κ B inhibitory effect representing approximately 2.5 times superior activity compared with the control group.

[ Experimental Example  3. MMP -One mRNA  Expression inhibition effect measurement]

Matrix Metallo-Proteinase-1 is one of the major factors involved in skin aging and wrinkle formation. It breaks down the connective tissue that maintains skin elasticity by breaking down the components that make up the extracellular matrix and basement membrane. It causes wrinkle formation, deterioration of skin elasticity, promotes collagen breakdown of dermis layer, and plays a very important role in photoaging.

This MMP-1 is promoted by UVA expression, RT-PCR was performed to see the gene expression inhibitory effect of MMP-1.

Fibroblasts were aliquoted at a density of 1 × 10 ⁶ in a 60 mm dish and incubated at 37 ° C. for 24 hours. UVA was irradiated with 6J, and Examples 7 to 9 and Comparative Examples 7 to 9 were added thereto, followed by incubation at 37 ° C. for 24 hours.

Cell medium was removed and 1 ml of Trizol (Trizol, invitrogen, USA) was added and left at room temperature for 5 minutes. After adding 200 μl of chloroform, the mixture was left at room temperature for 5 minutes and centrifuged at 13,000 rpm for 20 minutes. The supernatant was carefully transferred to a new tube and 500 µl of isopropyl alcohol was added. After standing at room temperature for 5 minutes, centrifuged at 13,000 rpm for 20 minutes to remove isopropyl alcohol, and then adding 1 ml of 75% ethanol, centrifuged at 10,000 rpm for 10 minutes, and then removing ethanol. The pellet was completely dried and then dissolved by adding 20 µl of sterile purified water. RNA was quantified at 260 nm using an ultraviolet detector, followed by reverse transcription-polymerase chain reaction. Reverse transcription polymerization reaction was used in the All-in-one reverse transcription polymerization kit (All-in-one RT-PCR kit, SuperBio, Korea), the primers and reaction conditions are shown in Tables 6 and 7 below, provided in the all-in-one reverse transcription polymerization kit The experiment was carried out according to the manual.

Table 6 MMP-1 Primer

MMP-1 Primer sense 5'-TGGGAGCAAACACATCTGA-3 ' Antisense 5'-ATCACTTCTCCCCGAATCGT-3 ' Reaction condition Reverse transcription at 50 ° C for 30 minutes, then inactivate reverse transcription enzyme at 96 ° C for 3 minutes, polymerization at 94 ° C for 1 minute, 48 ° C for 1 minute, and 72 ° C for 1 minute at 25 cycles

Table 7 Actin Primer

Actin primer sense 5'-GAGACCTTCAACACCCCAGCC-3 ' Antisense 5'-GGCCATCTCTTGCTCGAAGTC-3 ' Reaction condition Reverse transcription at 50 ° C for 30 minutes and then inactivation of reverse transcription enzyme at 96 ° C for 3 minutes, polymerization at 25 cycles of 94 ° C for 1 minute, 48 ° C for 1 minute, and 72 ° C for 1 minute

The result of measuring the inhibitory effect of MMP-1 expression was shown by correcting the actin by the following formula.

MMP-1 expression level (corrected value for actin,%) = [(actin expression of experimental group / actin expression of control group) X (amount of MMP-1 expression of experimental group)] / amount of MMP-1 expression of control group) ] X 100

As shown in Table 8 and FIG. 3, the oyster barberry fruit extract was found to inhibit concentration-dependently the activity of MMP-1 induced by UVA. In addition, the combination of oyster bark fruit extract and golden extract acacia extract appeared to be more effective in inhibiting the activity of MMP-1 induced by UVA.

Table 8 MMP-1 mRNA expression inhibition effect

MMP-1 expression level (corrected for% actin) Control 100 Comparative Example 7 75.5 Comparative Example 8 36.1 Comparative Example 9 27.5 Example 7 46.3 Example 8 22.7 Example 9 20.1

[ Experimental Example  4: test for improving skin wrinkles on human skin]

Subjects were selected to have wrinkles around their eyes by a dermatologist's questionnaire and physical examination. The eye area wrinkle of each subject was determined as the test site.

The recruited subjects were randomly assigned to four groups (test group 1, test group 2, test group 3, control group) of 24 subjects each. Twice a day (morning, evening), 12 weeks, so that the nutrition cream of Comparative Example 10 and the nutrition cream prepared in Examples 10 to 12 were applied to the subjects so as to bleed well with the amount of wrinkles around the eyes. It was applied to the control and test groups 1 to 3, respectively.

The skin wrinkle improvement effect was measured before and after using the product (0 week), 6 weeks, and 12 weeks after taking skin stability for 30 minutes under constant temperature and humidity conditions (22 ℃ ± 2 ℃, 40 ~ 60%), Replica was prepared and image analysis was performed on skin wrinkle replica using visometer (Visiometer, Skin-Visiometer SV 600, Courage & Khazaka, Germany). Wrinkle analysis analyzed the change in the wrinkle depth of the skin by grasping the depth of the wrinkles by the light and shade of the shadow generated by irradiating a certain angle of light to the mock plate, the results are shown in Table 9 and FIG.

As the depth of wrinkles decreases, skin wrinkles are improved and the depth of wrinkles is lowered. The unit is an arbitrary unit.

Table 9: Effect of improving skin wrinkles on human skin

Depth of wrinkle (arbitrary unit) Week 0 6 Weeks 12 Weeks Control group (Comparative Example 10) 0.56 ± 0.14 0.59 ± 0.16 0.58 ± 0.10 Test group (Example 10) 0.57 ± 0.11 0.56 ± 0.10 0.56 ± 0.10 Test group (Example 11) 0.56 ± 0.10 0.55 ± 0.10 0.52 ± 0.08 Test group (Example 12) 0.57 ± 0.13 0.53 ± 0.11 0.48 ± 0.10

^{NOTE The} above figures are expressed as mean ± standard deviation.

From the results shown in Table 9 and Figure 4, it can be seen that the combination of the golden extract, acacia extract and oyster bark fruit extract has an excellent anti-wrinkle effect.

The composition of the present invention can be prepared in the following formulations, the following formulations are merely illustrative of the present invention, whereby the content of the present invention is not limited.

Formulation Example 1. Lotion

3 parts by weight of glycerin, 2 parts by weight of butylene glycol, 2 parts by weight of propylene glycol, and a preservative were added to the purified water, followed by stirring to dissolve. Then, 1 part by weight of Tween 20 was dissolved by heating to 60 ° C., and then perfume was added. Stirred to 80 parts by weight of purified water. Finally, 2 parts by weight of the blend powder of aloesine and licorice extract and 10 parts by weight of ethanol were added thereto, followed by sufficiently stirring to obtain the title product.

Formulation Example 2 Milk Lotion

Cytosterol 1.5 parts by weight, 1.5 parts by weight of polyglycerol 2-oleate, 1 part by weight of ceramide, 1 part by weight of ceteareth-4 and 1.5 parts by weight of cholesterol are homogenized at a constant temperature, and aloesine, licorice extract and oilseed rape 2 parts by weight of the powder mixture of the extract, 0.4 parts by weight of dicetyl phosphate, 4.0 parts by weight of concentrated glycerin, 10 parts by weight of sunflower oil, 0.3 parts by weight of carboxyvinyl polymer, 0.3 parts by weight of xanthan gum and a preservative by heat dissolution. 76.5 parts by weight of purified water was added to the solution, followed by emulsification in a homogenizer. An appropriate amount of perfume was added thereto, followed by stirring and mixing to obtain the title product.

Formulation Example 3 Nutrition Essence

Cytosterol 1.7 parts by weight, polyglycerol 2-oleate 1.5 parts by weight, ceramide 0.7 parts by weight, ceteareth-4 1.2 parts by weight, cholesterol 1.5 parts by weight homogenized at a constant temperature, aloesine, licorice extract, rape flowers 5 parts by weight of the powder of the extract and the golden extract, 0.4 part by weight of dicetyl phosphate, 5.0 parts by weight of concentrated glycerin, 15 parts by weight of sunflower oil, 0.2 parts by weight of carboxyvinyl polymer, 0.2 parts by weight of xanthan gum and preservative 67.6 parts by weight of purified water was added to the solution, followed by emulsification in a homogenizer, and then an appropriate amount of perfume was added thereto, followed by stirring and mixing to obtain the title product.

Claims (9)

Cosmetic composition comprising a golden extract and acacia extract. The cosmetic composition according to claim 1, further comprising an oyster barberry extract. A cosmetic composition for preventing skin aging or improving wrinkles, comprising the blended plant extract of claim 1. The cosmetic composition according to claim 1, wherein the golden extract and the acacia extract are in a weight ratio of 0.1 to 10: 0.1 to 10. The cosmetic composition according to claim 2, wherein the golden extract, the acacia extract and the oyster tree fruit extract are in a weight ratio of 0.1 to 10: 0.1 to 10: 0.1 to 10. The cosmetic composition according to claim 1 or 2, wherein the total content of the blended plant extract is 0.001 to 90% by weight in dry weight relative to the total weight of the composition. The cosmetic composition according to claim 1 or 2, wherein the total content of the blended plant extract is 0.5 to 30% by weight in dry weight relative to the total weight of the composition. The cosmetic composition according to claim 1 or 2, wherein the golden extract, acacia extract and oyster fruit extract are ethanol extracts. Naturally drying golden roots, acacia heart and leaves, or oyster fruit; Extracting by adding 1 to 10 times ethanol to the dry weight; Filtering it; And Method for producing a plant extract comprising the step of concentration under reduced pressure.