KR20070076656A - A cosmetic composition containing as available ingredient the extracts of pinus koraiensis - Google Patents

Abstract

Provided is a cosmetic composition which shows excellent keratinocyte growth promoting effect, active oxygen scavenging effect, biosynthesis promoting effect of type 1 pro-collagen through biosynthesis decrease of MMP-2, anti-aging and skin moisturizing effect. The composition comprises an extract of Pinus koraiensis and at least one extract selected from the group consisting of an extract of Berberis Koreana Palbin, an extract of Phaseoli angularis Semen, an extract of Chinese quince, and an extract of black sesame. The extract is obtained by using water as an extraction solvent. The composition further comprises 1,3-butyleneglycol.

Description

A cosmetic composition containing as available ingredient the extracts of Pinus koraiensis}

The present invention relates to a cosmetic composition further comprising at least one selected from the group consisting of barberry extract, red soybean extract, quince extract and giant extract as an active ingredient. In more detail, the cosmetic composition according to the present invention further contains one or more selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract and giant extract in the pine cone extract as an active ingredient, excellent keratinocyte growth promoting effect, It shows active oxygen scavenging effect, promoting biosynthesis of type 1 procollagen by reducing biosynthesis of MMP-1, anti-aging effect and skin moisturizing effect.

Skin is the body's primary protective film that protects organs in the body from temperature and humidity changes, stimuli from the external environment such as UV and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, ultraviolet rays, excessive physical and chemical stimuli from the outside, stress, malnutrition, etc., lowers the normal function of the skin and promotes skin aging such as loss of elasticity, keratinization and wrinkle formation.

In particular, keratinocytes are made in turn, and the outermost layers of old keratinocytes sometimes deviate, so if the keratinization process that maintains a constant thickness of the epidermis is not smooth, abnormal keratinocytes are produced and accumulated on the skin surface. The thickened skin becomes rough and dull, fine wrinkles are generated, and eventually the skin ages. In addition, the decrease in biosynthesis of type 1 procollagen through the biosynthesis of MMP-1 by ultraviolet rays related to the decomposition of free radicals and skin substrates produced by ultraviolet rays is also associated with skin aging.

Therefore, in order to prevent such a phenomenon and maintain a healthier and more elastic skin, by using cosmetics fortified with conventional bioactive substances obtained from various animals, plants, microorganisms, etc., it is possible to maintain the unique function of the skin and activate skin cells. Efforts have been made to effectively suppress skin aging. However, conventional cosmetic raw materials have many problems, such as most of them are ineffective or cause skin side effects.

Accordingly, the present inventors have tried to find a raw material having a skin moisturizing effect and skin anti-aging effect without causing skin side effects among natural products, the group consisting of barberry extract, red soybean extract, Chinese quince extract and seunggi extract in the sea berry extract The composition further contains at least one selected from keratinocyte growth promoting effect, free radical scavenging effect, reducing the biosynthesis of MMP-1, promoting the biosynthesis of type 1 procollagen, anti-aging and skin moisturizing effect. And the present invention was completed.

Accordingly, an object of the present invention is an active ingredient containing at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract and giant extract as an active ingredient keratinocyte growth promoting effect, free radical scavenging effect, MMP It is to provide a cosmetic composition having a biosynthesis promoting effect, anti-aging effect and skin moisturizing effect of type 1 procollagen through reduction of -1 biosynthesis.

In order to achieve the above object, the cosmetic composition according to the present invention is characterized in that it further contains at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract as an active ingredient. The cosmetic composition according to the present invention, by containing the active ingredient, excellent keratinocyte growth promoting effect, active oxygen scavenging effect, biosynthesis promoting effect of type 1 procollagen through the reduction of biosynthesis of MMP-1, anti-aging effect and skin moisturizing It is characterized by showing the effect.

Hereinafter, each active ingredient of the cosmetic composition according to the present invention will be described in detail.

Pinus koraiensis is a dried pine seed of a pine tree belonging to the pine family, and it is also known as white porcelain, songza, and white rice. It is contained in a pine cone-like conifer, and the seeds are triangular ovate. Since ancient times, Songsong has been used as a nourishing tonic, and it is known that it is good to eat during the recovery period. In addition, it is a high-calorie food rich in fat and protein, especially rich in vitamin B, softens skin and lowers blood pressure.

Prunus mume is the fruit of a plum tree belonging to the Rosaceae family and is called mainly plum. The oldest fruit tree is used in Chinese ancient Xinnongcho, which has been used for medicinal purposes. In oriental medicine, it is used as an herbal medicine that has effects on health, hemostasis, branch office, expectoration, poisoning, and detoxification. The barberry is an alkaline food, good for fatigue recovery, and constitution improvement. In particular, it is excellent in detoxification, good for skin care, and also has a bactericidal effect. In recent years, many studies have been made as anti-cancer food.

Phaeseolus angularis is a legume plant, also known as soybean, in the name of the red bean. The origin of red beans is estimated to be large in China, and has been grown for a long time in the East. In oriental medicine, it is used as a herbal medicine that shows the effects of diuresis, acid blood, phlegm, hygiene, swelling, dehumidification, chemostasis, detoxification, drainage, dry stomach, open heart beef, shoulder blades and wall warming.

Chaenomeles sinensis is the fruit of the Chinese quince, a cultivated plant native to China, which looks like a melon but is also called a tree because it runs on the tree. Main ingredients of Chinese quince include saponin, organic acid, flavonoids and tannins. In oriental medicine, it is used as a herbal medicine that has effects on the root, bow, flat, hwawi, wet, and pain.

Sesamum indicum has excellent effects on chronic gastroenteritis, neuritis, high blood pressure, constipation, tonic, anemia, and according to the herbology, it has been recorded to reduce cholesterol, improve blood circulation, and improve skin and hair. . In oriental medicine, it is used as a herbal medicine that is effective for yun jang, interpolated sinus, soobal, Myeongmok, Jeon Jung-soo, long-term meat, and dae-small intestine. The winner's protein is mainly globulin and contains a lot of gamma-tocopherol, which helps to prevent antioxidant activity. It prevents skin aging by antioxidant activity and activates skin metabolism to partially blacken pigmentation. Also helps.

The extracts contained in the cosmetic composition according to the present invention are conventionally used to extract the active ingredient from the plant and are prepared by methods well known to those skilled in the art. For example, water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate or diethyl ether having 1 to 3 carbon atoms are added to the medicinal herb powder in an amount of 1 to 15 parts by volume based on the dry weight of the ground product, and then the cooling capacitor Extracted by heating at 50-100 ° C. for 5 to 24 hours in an extractor to which is attached; Water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate or diethyl ether having 1 to 3 carbon atoms are added to the pulverized product in an amount of 1 to 15 parts by volume based on the dry weight of the pulverized product, and then at a temperature of 4 to 25 ° C. Soak for 3 to 20 days to obtain an extract; After adding an appropriate amount of water to anhydrous or hydrous ethanol or methanol extract, the resulting precipitate is filtered off, and then added with ethyl acetate, butanol or diethyl ether, mixed well and left to separate the layers, and then the upper layer is separated. The extract is obtained from an extractor with a cooling capacitor. The extraction filtrate of the herbal medicine extract extracted by the above method is concentrated under reduced pressure, dried, and then blended into the cosmetic composition.

The extract according to the present invention may be used by mixing separate extracts of each herbal medicine, or may be prepared by mixing the herbal medicines and then extracted at once.

The cosmetic composition according to the present invention further contains at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract in the total active ingredient, that is, the extract of the whole composition, the active ingredient is the total weight of the composition It is preferable to contain at a concentration of 0.0001 to 25% by weight relative to. This is because when the active ingredient is less than 0.001% by weight, the effect is insignificant, and when the active ingredient is more than 25% by weight, there is a problem in the usability of the product and the stability of the formulation.

The cosmetic composition according to the present invention shows excellent keratinocyte growth promoting effect, active oxygen scavenging effect, biosynthesis promoting effect of type 1 procollagen through reducing biosynthesis of MMP-1, anti-aging effect and skin moisturizing effect.

In addition, the cosmetic composition according to the present invention may contain, in addition to the above-mentioned extracts, other ingredients that can give a synergistic effect to the main effect, preferably within the range of not impairing the main effect of the present invention, In addition to the extract of the present invention, other components may be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or use purpose of other cosmetics.

For example, the cosmetic composition may contain a skin absorption promoting substance to increase the effect. In addition, the cosmetic composition of the present invention may include a material selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

In addition, the cosmetic composition of the present invention may include other ingredients which are usually formulated into cosmetics as necessary in addition to the essential components, and examples thereof include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments. Organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, limiting agents, purified water and the like.

The compounding component which may be included in the cosmetic composition of the present invention is not limited thereto, and the compounding amount of the above component is possible within the range not impairing the object and effect of the present invention, but preferably 0.01 to the total weight of the composition. 10 wt%, more preferably 0.01 to 5 wt%.

The cosmetic composition according to the present invention is not particularly limited in the formulation, and for example, emulsion, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence, Formulations such as cleansing foams, cleansing creams, cleansing water, soaps or sprays and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

Reference Example 1 Preparation of Herbal Medicine Extract

Haesongja, barberry, red soybeans, Chinese quince, Geoseungja (dry chemicals Co., Ltd.), each of the herbs were collected, selected only for high quality herbs, washed with water to remove impurities and dried in the shade. Next, take 100 g of each of the pine cones, barberry, red bean curd, Chinese quince, and queer, add 2 liters of purified water, and boil for 18 hours under a reflux apparatus at about 95-100 ° C. After the extraction was completed, the mixture was continuously concentrated by distillation and then cooled to room temperature until it became a total weight of about 500 g. Next, the mixture was put in a filter cloth and compressed to separate and remove the solids, and then filtered to obtain a viscous concentrate. The concentrate was concentrated under reduced pressure at 80 ° C. to completely remove the water, and then dried in vacuo to give an extract in the form of a powder solid (5.0 g of pine nuts, 10 g of barberry, 5.0 g of red bean curd, 5.0 g of quince, 5.0 g of queer).

[Examples 1-15 and Comparative Example 1]

Each herbal medicine water extract obtained in Reference Example 1 was mixed in the combination of Table 1 to prepare Examples 1 to 15 and Comparative Example 1 was used in the experiment. At this time, the mixing ratio of each extract was the same amount.

ingredient Example Comparative example One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 One Raider ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ Barberry ○ ○ ○ ○ ○ ○ ○ ○ Small cow ○ ○ ○ ○ ○ ○ ○ ○ Quince ○ ○ ○ ○ ○ ○ ○ ○ Winner ○ ○ ○ ○ ○ ○ ○ ○

 [Test Example 1] effect of growth promotion (MTT assay) of keratinocytes

The effects of the extracts of Examples 1 to 15 and Comparative Example 1 on the growth ability of keratinocytes were measured by MTT method, briefly explaining the method as follows.

First, the keratinocytes were planted in a 96 well plate at a concentration of 5 × 10 ³ per 200 μl per well and incubated for 24 hours, and then the extracts of Examples 1 to 15 and Comparative Example 1 were prepared in 0.0001, 0.001, After incubation for 48 hours with 0.01, 0.1% concentration, the culture solution was aspirated and removed, washed once with PBS and 100 μl of MTT (Methylthiazolyldiphenyl-tetrazolium bromide, Sigma, USA) (0.5 mg / mL) solution. Cells were added and incubated at 37 ° C., 5% CO ₂ for 4 hours. Then, the culture solution was aspirated and removed, and then 200 μl of DMSO (dimethyl sulfoxide) solution was added, shaken for 10 minutes in a shaker, and the absorbance at 540 nm was read with an ELISA reader (DIbiotech, Korea). . At this time, as a control group, an untreated group of extracts of Examples 1 to 15 and Comparative Example 1 was used. The results are shown in Table 2 below.

% 0.0001 0.001 0.01 0.1 Example 1 136 197 245 277 Example 2 136 197 245 277 Example 3 139 201 244 276 Example 4 137 199 247 278 Example 5 141 205 257 286 Example 6 141 210 255 285 Example 7 143 215 255 284 Example 8 145 217 260 287 Example 9 141 216 259 284 Example 10 155 219 265 285 Example 11 164 222 273 293 Example 12 173 225 277 297 Example 13 168 223 275 299 Example 14 169 219 279 299 Example 15 185 238 288 331 Comparative Example 1 135 185 235 251 Control 100 100 100 100

As shown in Table 2, it was found that the effect appears in all cases, the higher the concentration of the extract generally used, the growth promoting effect of keratinocytes also increased. In addition, the compositions of Examples 1 to 15, which further contain at least one selected from the group consisting of barberry extract, red soybean extract, quince extract, and giant extract, are more effective than the composition of Comparative Example 1 containing only the pine cone extract. It was found that the more the type of extract selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract, which is added to the extract of Haesongja, increases the growth promoting effect of keratinocytes accordingly.

Test Example 2 Active Oxygen Scavenging Effect

In order to determine the active oxygen generation inhibitory effect, that is, the active oxygen scavenging effect of the extracts of the extracts of Examples 1 to 15 and Comparative Example 1, the following experiment was performed using a fluorescent material.

First, the cell line used in the experiment was a human keratinocytes HaCaT cell line distributed from Dr. Fusenig of the German Cancer Research Center, which is a 96 well black plate for fluorescence measurement. Dispense at 2.0 × 10 ⁴ per well, and use Dulbeccos Modification of Eagles Medium, FBS 10%, Gibco, USA (DMEM) medium supplemented with penicillin / streptomycin at 37 ° C., 5% CO ₂ . After culturing for 1 day, the extracts of Examples 1-15 and Comparative Example 1 were 0.5, 0.25, 0.125, 0.0625, 0.03125% The concentration was treated.

Next, the test sample was added and incubated for 24 hours, and then washed with HCSS (HEPES-buffered control salt solution, Gibco, USA) to remove the remaining medium, and DCFH-DA (2 ', 7'-) prepared at 20 μM in HCSS. 100 μl of dichlorodihydro-fluorescein diacetate, Molecular Probes, USA) was incubated at 37 ° C. and 5% CO ₂ for 20 minutes and washed with HCSS. Thereafter, 100 μl of the treated HCSS was added to each sample concentration, and then the fluorescence of DCF (dichlorofluorescein) oxidized with active oxygen was measured by a fluorescent plate reader (Ex = 485 nm, Em = 530 nm). After UVB (30mJ / cm ² ) was irradiated and immediately after treatment (Table 3) and after 3 hours of treatment (Table 4) the fluorescence was measured with a fluorescence plate reader (Ex = 485 nm, Em = 530 nm). At this time, as a control group, an untreated group of extracts of Examples 1 to 15 and Comparative Example 1 was used.

% 0.5 0.25 0.125 0.0625 0.03125 Example 1 71.57 75.70 75.73 82.75 82.75 Example 2 71.49 75.68 75.70 82.70 82.71 Example 3 71.47 75.50 75.67 82.69 82.73 Example 4 71.39 75.51 75.65 82.71 82.70 Example 5 71.41 75.38 75.40 82.51 82.62 Example 6 71.36 75.27 75.41 82.52 82.65 Example 7 71.38 75.25 75.42 82.50 82.53 Example 8 71.38 75.23 75.31 82.32 82.55 Example 9 71.31 75.31 75.51 82.43 82.43 Example 10 71.33 75.27 75.50 82.41 82.41 Example 11 71.15 74.55 75.33 81.37 82.01 Example 12 70.87 74.57 76.15 81.37 81.96 Example 13 71.11 74.42 75.00 81.33 81.83 Example 14 70.94 74.41 75.01 81.30 81.81 Example 15 70.65 73.50 74.50 80.20 81.21 Comparative Example 1 72.49 76.25 76.26 83.61 83.73 Control 100 100 100 100 100

% 0.5 0.25 0.125 0.0625 0.03125 Example 1 61.05 68.88 73.63 80.83 80.50 Example 2 61.03 68.85 73.51 80.52 79.96 Example 3 61.02 69.01 73.60 80.55 80.65 Example 4 61.00 69.00 73.61 80.57 79.99 Example 5 60.97 69.05 73.02 80.52 79.99 Example 6 61.34 68.94 72.99 80.52 79.99 Example 7 60.85 68.93 73.01 80.51 80.43 Example 8 60.91 69.05 72.82 80.40 79.99 Example 9 61.00 69.00 73.64 80.41 80.45 Example 10 60.96 69.05 73.21 80.35 79.96 Example 11 60.55 68.62 71.61 79.50 79.95 Example 12 60.41 68.41 71.42 79.73 79.95 Example 13 60.27 68.42 71.41 79.71 79.94 Example 14 60.34 68.42 71.42 79.66 79.94 Example 15 59.37 66.31 71.16 78.57 78.71 Comparative Example 1 61.45 69.97 73.77 81.82 80.77 Control 100 100 100 100 100

As shown in Table 3 and Table 4, it was found that the active oxygen production inhibitory effect appeared in all the test samples, it was found that the higher the concentration of the extract used, the more active oxygen production is suppressed. In addition, the composition of Examples 1 to 15 which further contain at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract in the pine cone extract is more effective than the composition of Comparative Example 1 containing only pine cone extract It was found that the more active kinds of extracts selected from the group consisting of barberry extract, red soybean extract, quince extract and giant extract, which are added to the extract of Haesongja, are more inhibited accordingly. It was also found that the effect was higher after 3 hours of treatment than immediately after treatment.

 Test Example 3 MMP-1 and Procollagen Assay

MMP-1 and procollagen in order to investigate the effect of reducing the biosynthesis of MMP-1 by ultraviolet rays related to the decomposition of the skin substrate of the extracts of Examples 1 to 15 and Comparative Example 1 to promote the biosynthesis of type 1 procollagen The procedure (procollagen assay) was performed in the following manner.

First, the fat layer of the normal epidermis is removed and chopped to separate the epidermis and the dermis with collagenase, and then the epidermis and the dermis are put into 0.25% trypsin solution, respectively, and then in a 37 ° C. and 5% CO ₂ incubator. Treated for a minute. Since vortex was performed to release the keratinocytes and fibroblasts, respectively. After collecting and washing the free cells, keratinocytes were cultured at 1 × 10 ⁴ cells / cm ² in KGM (keratinocyte growth medium, Clonetics, USA), and fibroblasts were added with 10% fetal bovine serum (FBS). Cultured in DMEM medium. When grown at about 70-80%, the cells were subcultured at a ratio of 1: 3, and cells subjected to 3-4 passages were used for the experiment.

For experiments to measure the amount of MMP-1, fibroblasts were cultured in at least 90% in 48 well plates and then starved for one day. After washing twice with PBS, the lid of the plate (plate) was opened with 100 μl of PBS, and UV A (Dermlight cube 401 equipped with UVA filters, UVAtec, USA) was irradiated with 15 J / cm ² . Immediately after irradiation, washed once again with PBS, and added DMEM without fetal calf serum containing 0.001 and 0.01% of the extracts of Examples 1 to 15 and Comparative Example 1, respectively, at 37 ° C. and 5% CO ₂ incubator. After incubation for a time, the amount of free MMP-1 in the medium was measured using the MMP-1 human ELISA system (RPN2610, Amersham Pharmacia Biothch, UK) and corrected for the total protein amount of fibroblasts. (Table 5). At this time, as a control group, an untreated group of extracts of Examples 1 to 15 and Comparative Example 1 was used.

Meanwhile, in order to measure the amount of procollagen, the extracts of Examples 1 to 15 and Comparative Example 1 were 0.001 and 0.01%, respectively, without ultraviolet irradiation under starvation under the same conditions as the method of measuring the amount of MMP-1. After 24 hours, the amount of procollagen released in the medium was measured using the procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan). 6). As a control group, an untreated group of extracts of Examples 1 to 15 and Comparative Example 1 was used.

% 0.001 0.01 Example 1 93 77 Example 2 93 74 Example 3 88 72 Example 4 89 72 Example 5 87 61 Example 6 85 59 Example 7 83 61 Example 8 81 59 Example 9 83 60 Example 10 74 53 Example 11 74 54 Example 12 73 56 Example 13 75 55 Example 14 74 54 Example 15 70 51 Comparative Example 1 95 79 Control 100 100

% 0.001 0.011 Example 1 108 116 Example 2 108 115 Example 3 105 116 Example 4 109 116 Example 5 111 123 Example 6 114 124 Example 7 116 124 Example 8 115 127 Example 9 117 125 Example 10 126 136 Example 11 125 135 Example 12 125 137 Example 13 127 137 Example 14 127 135 Example 15 135 144 Comparative Example 1 103 111 Control 100 100

As shown in the MMP-1 inhibitory effect of Table 5, it was found that the MMP-1 inhibitory effect appeared in all the test samples, the higher the concentration of the extract used, the less the amount of MMP-1 produced Could know. In addition, the compositions of Examples 1 to 15 further contain at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract in the pine cone extract than the composition of Comparative Example 1 containing only pine cone extract It was found that the more the type of extracts selected from the group consisting of barberry extract, red soybean extract, quince extract, and giant extract added to Haesongja extract, the amount of MMP-1 produced decreased accordingly.

In addition, as shown in Table 6 to increase the biosynthesis of type 1 procollagen, it was found that the effect of increasing the biosynthesis of type 1 procollagen in all the test samples, the higher the concentration of the extract used in general The amount of collagen was found to increase. In addition, the compositions of Examples 1 to 15 further contain at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract in the pine cone extract than the composition of Comparative Example 1 containing only pine cone extract It was found that the more the type of extract selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract, added to the extract of Haesongja, the amount of procollagen produced increased accordingly.

 Formulation Examples 1-15 and Comparative Formulation Example 1 Preparation of Cosmetic Composition

The cosmetic compositions of Formulation Examples 1 to 15 and Comparative Formulation Example 1 were prepared by containing the extracts of Examples 1 to 15 and Comparative Example 1. The oil phase parts up to components 1 to 7 and the water phase parts up to components 8 to 15 were dissolved by heating in the compositions shown in Table 7 below, and then the oil phase parts were gradually added to the water phase and emulsified while stirring the water phase.

The cosmetic composition of Formulation Examples 1 to 15 is a composition further comprising one or more selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract as an active ingredient, while the cosmetic composition of Comparative Formulation Example 1 Only contains extracts of pine nuts. Each of the compositions was dissolved in oil and water phase at 65 ~ 75 ℃ completely, emulsified for 5 minutes at 7,000 to 8,000 rpm to prepare a cream formulation.

Component (Content; Weight%) Formulation Examples 1-15 Comparative Formulation Example 1 1.Beads wax 2 2 2. Stearyl Alcohol 3 3 3. Stearic acid 8 8 4. Squalene 10 10 5. Propylene Glycol Monostearate 3 3 6. Polyoxyethylene ether One One 7. Preservatives, Antioxidants Quantity Quantity 8. Glycerin 5 5 9. Propylene Glycol 4 4 10. Triethylamine One One 11. Sodium Polyacrylate 0.2 0.2 12. Purified Water To 100 To 100 13. Comparative Example 1 - 5 14. Examples 1-15 each 5 - 15. Carbomer Quantity Quantity

[Test Example 4] Wrinkle improvement effect

In order to determine the wrinkle improvement effect of the Formulation Example 15 and Comparative Formulation Example 1 was carried out as follows.

140 women in their 30s were divided into 7 sets of 20 people each morning, twice a day for 8 weeks for twice a day for 8 weeks on the designated eye areas, and the formulations of Formulation Example 15 and Comparative Formulation Example 1 were applied, and then a silicone replica was prepared. The wrinkles at the designated sites were measured with a visometer (SVI60, Courage + Khazaka electronic GmbH, Germany). As a control group, the untreated group of the cream of Formulation Example 15 and Comparative Formulation Example 1 was used. The results are shown in Table 8, which shows the average of each parameter value after 8 weeks minus the parameter value 8 weeks ago. In other words, the negative the value, the higher the wrinkle improvement effect.

division R1 R2 R3 R4 R5 Formulation Example 15 -0.23 -0.19 -0.11 -0.09 -0.07 Comparative Formulation Example 1 -0.10 -0.09 -0.05 -0.05 -0.03 Control 0.28 0.27 0.22 0.04 0.04 R1: The difference between the highest and lowest values of the wrinkled contours R2: The randomly divided wrinkles contours by 5 spaces Rn: Average of R1 values divided by 5 R1: The highest value of R1 values divided by 5 R4: The top of each line at the baseline of the wrinkled contours R5 minus values of and valleys R5: Mean value of base line of wrinkle contour line minus each wrinkle profile

As can be seen in Table 8, the skin wrinkles of the composition of Formulation Example 15 containing all of the barberry extract, red soybean extract, Chinese quince extract and seunggi extract compared to the composition of Comparative Formulation Example 1 containing only the pine cone extract It was confirmed that the improvement effect was greatly enhanced.

 [Test Example 5] keratinocyte differentiation promoting effect

Human keratinocytes were placed in a culture flask, cultured, and grown to about 80%, and then the samples of Table 9 were each added at a concentration of 5 ppm and treated for 4 days. Next, a denatured protein such as urea or sodium dodecyl sulfate (SDS) and a reducing agent such as β-mercaptoethanol were mixed to remove the dissolved protein. Then, the peptide concentration was analyzed by measuring the absorbance at 310 nm of the remaining stratum corneum, and compared with the increase amount by each sample based on distilled water as a negative control group (0%) and the results are shown in Table 9 below.

matter Keratinocyte differentiation promoting effect Example 1 43 Example 2 47 Example 3 45 Example 4 43. Example 5 55 Example 6 57 Example 7 55 Example 8 57 Example 9 56 Example 10 58 Example 11 65 Example 12 68 Example 13 66 Example 14 68 Example 15 76 Comparative Example 1 32 Control group (distilled water) 0

From the results in Table 9, it was confirmed that the keratinocyte differentiation promoting effect of Examples 1 to 15 of the present invention is very excellent.

Formulation Examples 16-30 and Comparative Formulation Example 2 Preparation of Skin Moisturizing Cosmetic

The cosmetic compositions of Formulation Examples 16 to 30 and Comparative Formulation Example 2 were prepared by containing the extracts of Examples 1 to 15 and Comparative Example 1. After dissolving the water parts up to components 1 to 5 and the alcohol parts up to components 6 to 9 in the compositions shown in Table 10, respectively, at room temperature, alcohol parts were gradually added to the water phase while the water phase was stirred and solubilized. Next, stirring was continued for about 3-5 minutes after the addition of the alcohol parts was completed, followed by vacuum degassing to complete the preparation of the cosmetic.

Component (Content; Weight%) Formulation Examples 16-30 Comparative Formulation Example 2 1. Purified water To 100  To 100 2. Glycerin 3 3 3. Carbomer 0.2 0.2 4. Examples 1-15 each One - 5. Comparative Example 1 - One 6. Ethanol 3 3 7. Polyoxyethylene Hydrogenated Castor Oil 3 3 8. Incense Quantity Quantity 9. Preservative Quantity Quantity

Test Example 6 Skin Barrier Function Restoration Effect

In order to measure the skin barrier function recovery effect of Formulation Examples 16 to 30, acetone was repeatedly applied to the skin of hairless mice to impair barrier function, and then epidermal water loss (TEWL) was measured. When measured by servomed (Sweden) evaporimeter EP1 and reached 4.0 g / m 2 / h, the samples of Formulation Examples 16 to 30 were applied to an area of 5 cm 2, and the amount of epidermal moisture was lost for 1 hour, 2 hours, 4 hours, The extent of barrier function recovery was assessed by assessing the extent of reduction after 8 hours. At this time, a known lipid mixture (2: 1: 1 mixture of ceramide: cholesterol: fatty acid) was used as a positive control. The results are shown in Table 11 below comparing the change over time based on the amount of epidermal moisture loss in the initial damaged barrier state (100%).

Epidermal moisture loss (%) sample 1 hour later 2 hours later 4 hours later 8 hours later Formulation Example 16 69 60 49 35 Formulation Example 17 64 51 48 37 Formulation Example 18 70 61 49 36 Formulation Example 19 69 59 47 37 Formulation Example 20 62 54 41 27 Formulation Example 21 60 55 40 30 Formulation Example 22 61 51 37 29 Formulation Example 23 59 50 41 31 Formulation Example 24 57 55 39 31 Formulation Example 25 61 55 43 27 Formulation Example 26 55 49 35 24 Formulation Example 27 57 50 39 26 Formulation Example 28 56 47 35 26 Formulation Example 29 57 51 35 24 Formulation Example 30 50 43 36 21 Comparative Formulation Example 2 88 80 75 70 Geological Mixture (Yangseong Control) 64 43 37 26

As shown in the results of Table 11, the formulation example further contains at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, seunggi extract than the composition of Comparative Formulation Example 2 containing only the pine cone extract The composition of the 16 ~ 30 is more effective, the more the type of extract selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, seunggi extract extract added to the extract of the barrier function was higher.

Hereinafter, the formulation examples of the cosmetic composition according to the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

<Soap type>

ingredient Content (% by weight) Each of the extracts of Examples 1-15 1.00 (%) maintain A reasonable amount Sodium hydroxide A reasonable amount Sodium chloride A reasonable amount Spices Small quantity Purified water To 100

Lotion Formulation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 3.00 L-ascorbic acid-2-magnesium phosphate 1.00 Water Soluble Collagen (1% Aqueous Solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 Licorice Extract 0.20 1,3-butylene glycol 3.00 Purified water To 100

Cream formulation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 1.00 Polyethylene Glycol Monostearate 2.00 Self-emulsifying glycerin monostearate 5.00 Cetyl alcohol 4.00 Squalene 6.00 Glyceryl tri2-ethylhexanoate 6.00 Sphingolipid 1.00 1,3, -butylene glycol 7.00 Purified water To 100

<Pack formulation>

ingredient Content (% by weight) Each of the extracts of Examples 1-15 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-magnesium phosphate 1.00 Lauroylhydroxyproline 1.00 Water Soluble Collagen (1% Aqueous Solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water To 100

<Solutions Formulation>

ingredient Content (% by weight) Each of the extracts of Examples 1-15 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 13-butylene glycol 6.00 Dark glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 Purified water To 100

As described above, the cosmetic composition according to the present invention contains an at least one selected from the group consisting of barberry extract, red soybean extract, Chinese quince extract, and giant extract in Haesongja extract as an active ingredient, the growth promoting effect of excellent keratinocytes The active oxygen scavenging effect generated by ultraviolet rays, the effect of reducing the biosynthesis of MMP-1 by ultraviolet rays related to the decomposition of the skin substrate, promoting the biosynthesis of type 1 procollagen, anti-aging effect and skin moisturizing effect, The composition can be usefully used in cosmetics as a composition for promoting growth of keratinocytes, a composition for scavenging free radicals, a composition for promoting biosynthesis of type 1 procollagen by reducing the biosynthesis of MMP-1, a composition for preventing skin aging, and a composition for moisturizing the skin. have.

Claims (8)

Cosmetic composition for promoting growth of keratinocytes, characterized in that the extract contains more than one selected from the group consisting of barberry extract, red bean curd extract, Chinese quince extract and giant extract. An active oxygen scavenging cosmetic composition further comprising at least one selected from the group consisting of barberry extract, red soybean extract, quince extract, and giant extract as an active ingredient. For promoting the biosynthesis of type 1 procollagen through the reduction of biosynthesis of MMP-1, characterized in that it further contains at least one selected from the group consisting of barberry extract, red soybean extract, quince extract and giant extract as an active ingredient Cosmetic composition. A skin composition for preventing skin aging, characterized in that the extract further contains at least one selected from the group consisting of barberry extract, red soybean extract, quince extract and giant extract. Skin moisturizing cosmetic composition, characterized in that it further contains at least one selected from the group consisting of barberry extract, red soybean extract, quince extract and giant extract as an active ingredient. The cosmetic composition according to any one of claims 1 to 5, wherein the extract is extracted using water as an extraction solvent. 7. The cosmetic composition according to claim 6, wherein the composition further contains 1,3-butylene glycol. The cosmetic composition according to any one of claims 1 to 5, wherein the extract contains 0.0001 to 25% by weight based on the total weight of the composition.