JP7338842B2 - wrinkle-improving composition and wrinkle-improving skin cosmetic - Google Patents

Description

TECHNICAL FIELD The present invention relates to a wrinkle-reducing composition obtained by combining ingredients derived from natural products, and a wrinkle-reducing skin cosmetic containing the composition.

The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrix such as collagen and elastin, which are outside these cells and support the skin structure. In young skin, the proliferation of fibroblasts is active, and the interaction of skin tissue such as fibroblasts and extracellular matrix components maintains homeostasis, ensuring flexibility, elasticity, etc., and improving the appearance of the skin. It is maintained in a state of tension and luster.

However, under the influence of certain external factors such as ultraviolet irradiation, extreme dryness of the air, excessive skin washing, etc., or with advanced aging, collagen, elastin, etc., which are the main constituents of the extracellular matrix, decrease. As the amount of production decreases, it causes decomposition and alteration. As a result, the flexibility and elasticity of the skin are reduced, the skin loses its firmness and luster, and the skin exhibits aging symptoms such as wrinkles.

A wide variety of ingredients have been proposed as ingredients that suppress the formation of wrinkles and ingredients that improve wrinkles (see, for example, Patent Documents 1 and 2), but the demand for new active ingredients is still great at present. be. Under such circumstances, the standardization of wrinkle improvement evaluation is also underway (see Non-Patent Documents 1 and 2).

JP-A-09-255548 JP-A-2005-008571

"Anti-wrinkle Product Evaluation Guidelines for Obtaining New Efficacy", Journal of Japanese Cosmetic Science Society, 2006, vol.30, pp.316-332 “Guidelines for Evaluation of Anti-wrinkle products”, Journal of Japanese Cosmetic Science Society, 2007, vol.31, pp.411-431

An object of the present invention is to provide a novel composition having an excellent anti-wrinkle effect, and an anti-aging skin cosmetic containing the composition.

As a result of intensive studies by the present inventors in order to achieve the above object, it was found that the licorice leaf extract is combined with a predetermined natural product-derived component to provide an even more excellent wrinkle-improving effect, and the present invention. was completed.

Specifically, the present invention is as follows.
[1] a licorice leaf extract;
A wrinkle-improving composition comprising a combination of at least one of the following four types: Angelica keiskei extract, Chrysanthemum extract, Yokuinin extract and pearl protein hydrolyzate.
[2] The wrinkle-improving composition according to [1], which is characterized by combining the Angelica Angelica extract, the Chrysanthemum extract, the Yokuinin extract, and the pearl protein hydrolyzate.
[3] A wrinkle-reducing skin cosmetic comprising the wrinkle-reducing composition of [1] or [2].

According to the present invention, by combining a licorice leaf extract with a predetermined natural product-derived component, a novel composition excellent in wrinkle-improving action and an anti-aging skin cosmetic containing the composition are provided. can be done.

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below.
A wrinkle-improving composition according to an embodiment of the present invention is a combination of licorice leaf extract and at least one of the following four types: Angelica keiskei extract, Kinginka extract, Yokuinin extract, and pearl protein hydrolyzate. It will be.
A wrinkle-reducing skin cosmetic according to another embodiment of the present invention contains the wrinkle-reducing composition.

Here, in the present embodiment, the "extract" includes an extract obtained from the plant as an extraction raw material, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, or any of these Both crude and purified products are included.

The wrinkle-reducing composition according to the present embodiment can exert a wrinkle-reducing effect by utilizing the action of the licorice leaf extract, which is an essential ingredient.
The present inventors have searched for active ingredients combined with licorice leaf extract in order to further enhance the effectiveness of licorice leaf extract. The inventors have found that the wrinkle-improving effect of the licorice leaf extract can be more effectively exhibited by combining it with at least one kind of licorice leaf extract, leading to the configuration of the present embodiment.
The present embodiment will be described in detail below.

[Production of licorice leaf extract, Angelica keiskei extract, Chrysanthemum extract, Yokuinin extract]
In the present embodiment, the raw materials for extraction used to produce the licorice leaf extract, Angelica angelica extract, Kinginka extract, and Yokuinin extract are licorice, Angelica acutiloba Kitagawa, and Lonicera japonica Thunberg. ), and Yokuinin (name of crude drug).

Licorice is a perennial herb belonging to the genus Glycyrrhiza of the legume family. Licorice includes Glychyrrhiza glabra, Glychyrrhiza inflata, Glychyrrhiza uralensis, Glychyrrhiza aspera, Glychyrrhiza eurycarpa, Glychyrrhiza paridiflora. za pallidiflora ), Glychyrrhiza yunnanensis, Glychyrrhiza lepidota, Glychyrrhiza echinata, Glychyrrhiza acanthocarpa, etc., any of these may be used as the raw material for extraction, but it is particularly preferred to use Glychyrrhiza glabra, Glychyrrhiza uralensis, and Glychyrrhiza inflata as the raw material for extraction.

In the present embodiment, the component part of licorice used as the raw material for extraction is the leaf part. Here, the root of licorice has been used for medicinal purposes or as a raw material for sweeteners since ancient times, and is known to contain glycyrrhizin and other useful ingredients. However, in this embodiment, as a result of paying attention to the residue after using the root, the extract from the leaf of licorice is used. Glycyrrhizin and other useful ingredients contained in the root are not contained in the leaves of licorice.

Angelica acutiloba Kitagawa is a perennial plant native to Japan belonging to the family Umbelliferae and the genus Shishiudo, and can be easily obtained from these regions. Constituent parts of Angelica keiskei that can be used as raw materials for extraction include, for example, leaves, stems, flowers, fruits, roots, rhizomes, aerial parts, and mixtures thereof, preferably roots.

Kinginka (Lonicera japonica Thunberg., also known as honeysuckle) is an evergreen climbing woody plant belonging to the family Lonicera family, Lonicera genus. can. The parts that can be used as raw materials for extraction include, for example, leaves, stems, flowers, buds, fruits, pericarp, kernels, aerial parts, whole plants, and mixtures thereof, etc., but are preferred. are leaves, stems, flowers or buds.

Yokuinin (name of crude drug) is the seed portion of Coix lachrymal-jobi L. (scientific name: Coix lachrymal-jobi L.). Hatomugi is a plant native to the Indochina region of China, and is an annual plant belonging to the genus Juzdama of the Poaceae family, which is cultivated in the warm regions of the southwestern part of Japan, and can be easily obtained from these regions. Yokuinin has been conventionally used for purposes such as water replenishment, dehumidification, heat purification, purulent drainage, depletion, splenic pain relief, and the like.

The extract from the above plant can be obtained by drying the raw material for extraction, either as it is or pulverizing it using a coarse crusher and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. In addition, it may be used as an extraction raw material after being subjected to pretreatment such as degreasing with a non-polar solvent such as hexane. By performing pretreatment such as degreasing, the extraction treatment with a polar solvent can be efficiently performed.

As the extraction solvent, it is preferable to use a polar solvent, for example, water, hydrophilic organic solvents, etc., which are used alone or in combination of two or more at room temperature or at a temperature below the boiling point of the solvent. is preferred.

Water that can be used as an extraction solvent includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Treatments applied to water include, for example, purification, heating, sterilization, filtration, ion exchange, osmotic adjustment, buffering, and the like. Therefore, water that can be used as an extraction solvent in the present embodiment includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered physiological saline, and the like.

Hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

When using a mixture of two or more polar solvents as the extraction solvent, the mixture ratio can be adjusted as appropriate. For example, when a mixture of water and a lower aliphatic alcohol is used as the extraction solvent, the mixing ratio of water and the lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), More preferably, it is 7:3 to 2:8 (volume ratio). When using a mixture of water and lower aliphatic ketone, the mixing ratio of water and lower aliphatic ketone is preferably 9:1 to 2:8 (volume ratio). When using a mixed solution with a hydric alcohol, the mixing ratio of water and polyhydric alcohol is preferably 5:5 to 1:9 (volume ratio).

The extraction treatment is not particularly limited as long as the soluble components contained in the raw material for extraction can be eluted into the extraction solvent, and can be carried out according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent of 5 to 15 times the amount (mass ratio) of the extraction raw material, the soluble component is extracted at room temperature or under reflux heating, and then filtered to remove the extraction residue. You can get the liquid. A paste-like concentrate is obtained by distilling off the solvent from the obtained extract, and a dried product is obtained by further drying the concentrate.

The licorice leaf extract, Angelica keiskei extract, Chinginka extract, and Yokuinin extract obtained as described above are combined in a predetermined manner (more preferably, in combination with the pearl protein hydrolyzate described later) to improve wrinkles. Since it has an improvement effect, it can be used in a composition for improving wrinkles.

[Production of pearl protein hydrolyzate]
The pearl protein hydrolyzate used in the present embodiment is obtained by subjecting shells and/or pearls of nacre-bearing oysters to a demineralization treatment and hydrolyzing the pearl protein (conchiolin) obtained thereby.

Examples of shellfish having a mother-of-pearl include Akoya oyster (scientific name: Pinctada fucata) belonging to the family Mytilidae; Mytilus (scientific name: Mytilus coruscum) and Mussel (scientific name: Mytilus edulis) belonging to the family Mytilidae; (scientific name: Hyriopsis schlegelii), mussel (scientific name: Cristaria plicata), etc. Among these, pearl oysters are preferred.

Shells and/or pearls of nacreous oysters are subjected to a demineralization treatment as is or after crushing. The demineralization treatment of shells and/or pearls may be carried out by a conventional method, for example, an acid or an aqueous solution thereof is added to the shells and/or pearls and stirred.
The acid that can be used for the demineralization treatment is not particularly limited as long as it can sufficiently dissolve inorganic components such as calcium carbonate contained in shells and/or pearls. Examples include hydrochloric acid, sulfuric acid, inorganic acids such as phosphoric acid and nitric acid; organic acids such as oxalic acid, carbonic acid, citric acid, malic acid, maleic acid, tartaric acid, acetic acid and formic acid; Alternatively, two or more mixed acids may be used.
The amount of the acid or its aqueous solution to be added may be an amount that allows the inorganic components such as calcium carbonate contained in the shells and/or pearls to be sufficiently eluted, and may be appropriately set according to the type of acid used.

The treated liquid obtained by the decalcification treatment is subjected to solid-liquid separation means such as centrifugation, filtration, and decantation to recover insoluble matter, and if necessary, purified water is added to the recovered insoluble matter and centrifuged again. Washing operations such as separation and filtration are repeated to recover insoluble matter. Then, the obtained insoluble matter can be finely pulverized using a pulverizer or the like, if desired, and dried by a conventional method to obtain pearl protein (conchiolin).

Then, the obtained pearl protein (conchiolin) is hydrolyzed using an acid or alkali aqueous solution.
Acids that can be used for the hydrolysis treatment of the pearl protein (conchiolin) include, for example, hydrochloric acid and sulfuric acid. may be used.
Alkali that can be used for the hydrolysis treatment of pearl protein (conchiolin) include, for example, sodium hydroxide and potassium hydroxide. More than one species may be mixed and used.

When hydrolyzing using an acid or alkali aqueous solution, the amount of the acid or alkali aqueous solution added and the concentration of the acid or alkali in the aqueous solution may affect the hydrolysis of the pearl protein (conchiolin) contained in the shell and/or pearl. However, the amount may be sufficient as long as it cannot be completely decomposed into amino acids, and the amount of the aqueous solution added and the concentration of the acid or alkali in the aqueous solution can be appropriately changed according to the type of acid or alkali used. .
The hydrolysis treatment can be carried out under temperature conditions of 0 to 130° C., preferably 50 to 110° C., for about 30 minutes to 10 days, preferably about 5 hours to 5 days.

Acids or alkalis are removed from the solution thus obtained by hydrolysis treatment, and if necessary, ultrafiltration treatment is performed to remove high-molecular peptides, and further treatment such as concentration and drying is performed. to obtain a pearl protein hydrolyzate.
Acids or alkalis can be removed from the solution obtained by the hydrolysis treatment by conventional methods, such as freeze-drying and desalting by neutralizing the acid or alkali in the solution.

The pearl protein hydrolyzate obtained as described above exhibits an excellent wrinkle-improving effect when combined with the licorice leaf extract (more preferably, with the Angelica keiskei extract, the Chrysanthemum extract and the Yokuinin extract). Therefore, it can be used in a wrinkle-improving composition.

[Composition for improving wrinkles]
The wrinkle-improving composition according to the present embodiment is obtained by combining a licorice leaf extract with at least one of four types: Angelica keiskei extract, Kinginka extract, Yokuinin extract, and pearl protein hydrolyzate. (hereinafter sometimes referred to as a “mixture of ingredients derived from natural products”).
The wrinkle-improving composition of the present embodiment can be used in a wide range of applications such as pharmaceuticals, quasi-drugs, and cosmetics.

Wrinkles are formed due to a decrease in the flexibility and elasticity of the skin, but the decrease in the flexibility and elasticity of the skin is associated with a decrease in the amount of production of collagen, elastin, etc., which are the main constituents of the extracellular matrix. Decrease, their decomposition and alteration are important factors. Above all, the decrease in type I collagen, which is the main constituent of the dermis, is closely related to the decrease in skin flexibility and elasticity, and eventually to the formation of wrinkles.
In contrast, the wrinkle-improving composition according to the present embodiment contains a licorice leaf extract as an essential component. The licorice leaf extract has an excellent type I collagen production-promoting effect on skin fibroblasts, and also has a growth-promoting effect on skin fibroblasts, a moisturizing/barrier function-improving effect, and an antioxidant effect. ing. In the present embodiment, it is recognized that the type I collagen production-promoting action of the licorice leaf extract contributes to the wrinkle-improving effect.
Then, in the present embodiment, by combining at least one of the four types of Angelica keiskei extract, Chingincha extract, Yokuinin extract and pearl protein hydrolyzate with the licorice leaf extract, the effect of the licorice leaf extract is obtained. The properties are remarkably improved, and a composition having a particularly excellent anti-wrinkle effect can be obtained.

Angelica keiskei extract has the effect of promoting the production of basement membrane components such as claudin-1 and involucrin, as well as promoting the growth of skin fibroblasts, improving moisturizing and barrier functions, and anti-glycation of proteins. also have
Here, the basement membrane is present at the boundary between the epidermis and the dermis and not only connects the two but also plays an important role in maintaining skin functions. When basement membrane components decrease or structural changes occur in the basement membrane, the moisturizing function and elasticity of the skin decrease, which contributes to the formation of wrinkles.
In this embodiment, the function of the dermis is improved by the type I collagen whose production is promoted by the licorice leaf extract, and the function of the basement membrane is improved by the basement membrane component whose production is promoted by the angelica extract. It is considered that the wrinkle-improving effect is synergistically exerted by improving flexibility and elasticity.
When the Angelica keiskei extract is combined with the licorice leaf extract, the lower limit of the mass ratio of Angelica keiskei extract to 100 parts by mass of the licorice leaf extract is 30 parts by mass or more, from the viewpoint of facilitating synergistic wrinkle-improving effects. It is preferably 40 parts by mass or more, and particularly preferably 40 parts by mass or more. In addition, the upper limit of the mass ratio of the angelica extract is preferably 70 parts by mass or less, and particularly preferably 60 parts by mass or less.
In the present specification, all the mass ratios of natural product-derived components are in terms of solid content.

Snapdragon extract has an antioxidant effect and also has a protein anti-glycation effect. For this reason, when the prickly pear extract is combined with the licorice leaf extract, the type I collagen whose production is promoted by the licorice leaf extract becomes less susceptible to denaturation due to oxidation and saccharification, and the wrinkle-improving effect is exhibited more remarkably. is recognized.
In the case of combining the snapdragon extract with the licorice leaf extract, the lower limit of the mass ratio of the snapdragon extract to 100 parts by mass of the licorice leaf extract should be 5 parts by mass or more, from the viewpoint of exhibiting the wrinkle-improving effect more remarkably. is preferred, and 10 parts by mass or more is particularly preferred. Moreover, the upper limit of the mass ratio of the above-mentioned Kinginka extract is preferably 30 parts by mass or less, and particularly preferably 20 parts by mass or less.

Yokuinin extract has a moisturizing effect on the skin and a barrier function-improving effect, and further has an effect of promoting elastin production. Therefore, coix seed extract is recognized to restore skin flexibility and contribute to maintenance of skin homeostasis. By combining the yokuinin extract having these actions with the licorice leaf extract, the licorice leaf extract synergistically contributes to the promotion of type I collagen production, and the wrinkle-improving action is exhibited more remarkably. it is conceivable that.
When the coix extract is combined with the licorice leaf extract, the lower limit of the mass ratio of the coix seed extract to 100 parts by mass of the licorice leaf extract is 0.5 parts by mass, from the viewpoint of facilitating synergistic wrinkle-improving effects. It is preferably at least 2 parts by mass, and particularly preferably at least 2 parts by mass. The upper limit of the mass ratio of the Yokuinin extract is preferably 10 parts by mass or less, and particularly preferably 5 parts by mass or less.

The pearl protein hydrolyzate has antioxidative action, moisturizing action, protein anti-glycation action, skin fibroblast growth promoting action, etc., and also has a particularly excellent elastin production promoting action. Therefore, type I collagen whose production is promoted by the licorice leaf extract and elastin whose production is promoted by the pearl protein hydrolyzate restores the structure of the extracellular matrix in the skin (especially the dermis), resulting in skin flexibility. It is thought that the wrinkle-improving effect is exhibited more remarkably by improving the elasticity and elasticity.
When the pearl protein hydrolyzate is combined with the licorice leaf extract, the lower limit of the mass ratio of the pearl protein hydrolyzate to 100 parts by mass of the licorice leaf extract is 3 parts by mass, from the viewpoint of exhibiting the wrinkle-improving effect more remarkably. It is preferably 8 parts by mass or more, and particularly preferably 8 parts by mass or more. Moreover, the upper limit of the mass ratio of the pearl protein hydrolyzate is preferably 30 parts by mass or less, and particularly preferably 18 parts by mass or less.

At least one of the licorice leaf extract and Angelica extract, Chinginka extract, Yokuinin extract, and pearl protein hydrolyzate may be combined with the licorice leaf extract, but the wrinkle-improving effect is more pronounced. From the viewpoint of exhibiting the licorice leaf extract, it is preferable to combine two or more kinds, and it is particularly preferable to combine all four kinds.

The wrinkle-improving composition of the present embodiment may consist of only the combination of the above natural product-derived ingredients, or may be a formulation of the combination. In the case of formulating, a pharmaceutically acceptable carrier such as dextrin, cyclodextrin, etc., and any other adjuvant are used, and according to a conventional method, it is formulated into any dosage form such as powder, granules, tablets, liquid, etc. can be At this time, as auxiliary agents, for example, excipients, binders, disintegrants, lubricants, stabilizers, flavoring agents and the like can be used. The anti-wrinkle composition can be used by blending it with other compositions (for example, skin cosmetics to be described later), and can also be used as ointments, liquids for external use, patches, and the like.

When the wrinkle-improving composition of the present embodiment is formulated, the total content of the mixture of natural product-derived components in the wrinkle-improving composition is not particularly limited, and is appropriately set according to the purpose. can do.

In the wrinkle-improving composition of the present embodiment, if necessary, other natural extracts or the like having wrinkle-improving effects can be blended together with the mixture of ingredients derived from natural products and used as active ingredients.

Examples of the method of administering the anti-wrinkle composition of the present embodiment to a patient include transdermal administration, oral administration, etc. Depending on the type of disease, it is possible to appropriately select a suitable method for prevention, treatment, etc. It is possible, but transdermal administration is preferred.
In addition, the dose of the anti-wrinkle composition of the present embodiment may be appropriately increased or decreased depending on the type and severity of disease, individual differences among patients, administration method, administration period, and the like.

The wrinkle-reducing composition of the present embodiment can improve skin wrinkles and suppress the formation of wrinkles through the wrinkle-reducing action synergistically exerted by the mixture of ingredients derived from natural products. It can improve skin aging symptoms caused by wrinkle formation, and can prevent or treat skin aging symptoms caused by wrinkle formation.

The wrinkle-improving effect of the wrinkle-improving composition of the present embodiment is, for example, "anti-wrinkle product evaluation guidelines for acquiring new efficacy" (Non-Patent Document 1 mentioned above) and "Guidelines for Evaluation of Anti-wrinkle products" ( It can be evaluated by a method based on the above-mentioned Non-Patent Document 2) (these may be collectively referred to simply as “guidelines” below).
More specifically, the wrinkle-reducing composition according to the present embodiment can make wrinkles smaller and less noticeable due to its wrinkle-reducing action. In the analytical method, it can be confirmed as an effect of reducing parameters such as average wrinkle depth, maximum average wrinkle depth, maximum maximum wrinkle depth, and total wrinkle volume.
In addition, the wrinkle-reducing composition according to the present embodiment can smooth the surface of the skin due to its wrinkle-reducing action. value (arithmetic mean roughness of roughness curve), Ry value (maximum height of roughness curve).
In the present embodiment, among the above parameters, it is particularly preferable that at least one of the maximum average wrinkle depth, total wrinkle volume, and Ry value (maximum height of roughness curve) can be confirmed to be reduced.

The anti-wrinkle composition of the present embodiment has an excellent wrinkle-improving effect, and is therefore suitable for use in, for example, external preparations for skin. In this case, the wrinkle-improving composition to be blended may be blended with the mixture of the above natural product-derived components as it is, or may be formulated from the above-described mixture of natural product-derived components.

Here, the category of external preparations for skin is not limited, and includes a wide range of skin cosmetics, quasi-drugs used transdermally, pharmaceuticals, and the like, which will be described later.

Since the wrinkle-improving composition of the present embodiment has an excellent wrinkle-improving action, it can be suitably used as a reagent for research on these mechanisms of action.

[Skin cosmetics for improving wrinkles]
The anti-wrinkle composition according to the above embodiment exerts an excellent wrinkle-improving action based on the action of the mixture of ingredients derived from natural products, and therefore is suitable for blending in skin cosmetics. In this case, the wrinkle-improving composition to be blended may be blended with the above natural product-derived component mixture as it is, or may be formulated from the above natural product-derived component mixture. By blending the anti-wrinkle composition, the skin cosmetic can be suitably used for anti-wrinkle purposes.

The types of skin cosmetics in which the anti-wrinkle composition of the above embodiment can be blended are not particularly limited, and examples include ointments, creams, milky lotions, lotions, lotions, gels, beauty oils, packs, foundations, and the like. mentioned.

When the wrinkle-improving composition is blended into skin cosmetics, the blending amount can be appropriately adjusted according to the type of skin cosmetics. More preferably, the lower limit of the blending amount of the wrinkle-improving composition converted to the solid content of the mixture of natural product-derived ingredients is preferably about 0.0001% by mass or more, and about 0.001% by mass. % or more, and particularly preferably about 0.004 mass % or more. In addition, the upper limit of the amount of the wrinkle-improving composition converted to the mixture of the natural product-derived components is preferably about 10% by mass or less, more preferably about 1% by mass or less, and about It is particularly preferably 0.1% by mass or less.

As long as the wrinkle-improving action of the mixture of ingredients derived from natural products is not hindered, the skin cosmetic may contain main agents, auxiliaries, or other ingredients used in the production of ordinary skin cosmetics, such as astringents, bactericidal/antibacterial agents, and the like. agent, whitening agent, UV absorber, moisturizer, cell activator, anti-inflammatory/anti-allergic agent, antioxidant/active oxygen remover, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, interfaces Activators, fragrances and the like can be used in combination. Such combination may result in a more generalized product and may provide greater benefits than would normally be expected due to synergy between the other active ingredients used in combination.

The wrinkle-improving skin cosmetic of the present embodiment can improve skin wrinkles and suppress the formation of wrinkles through the wrinkle-improving action synergistically exerted by the mixture of ingredients derived from natural products. It is possible to improve skin aging symptoms caused by wrinkle formation, and to prevent or treat skin aging symptoms caused by wrinkle formation.

The embodiments described above are described to facilitate understanding of the present invention, and are not described to limit the present invention. Therefore, each element disclosed in the above embodiment is meant to include all design changes and equivalents that fall within the technical scope of the present invention.

Hereinafter, the present invention will be specifically described by showing formulation examples, test examples, etc., but the present invention is not limited to the following examples.

[Production Example 1] Production of anti-wrinkle composition The components shown below were mixed in a conventional manner to prepare an anti-wrinkle composition.
The five natural product-derived components used are all manufactured by Maruzen Pharmaceutical Co., Ltd. In addition, these blending amounts are solid content conversion values, and the solid content conversion values were measured according to the evaporation residue test method listed in the general test methods of the Standards for Quasi-drug Ingredients 2006.

[Formulation Example 1]
A lotion was prepared according to the following composition by a conventional method.
Wrinkle improvement composition (Production Example 1) 2.00% by mass
(Total amount of 5 natural product-derived components: 0.0120% by mass)
Citric acid 0.04% by mass
Sodium citrate 0.16% by mass
Disodium edetate 0.03% by mass
Methylparaben 0.15% by mass
PPG-13 decyltetradeceth-24 0.10 mass%
Phenoxyethanol 0.30% by mass
Xanthan gum 0.50% by mass
Remainder of purified water (total amount is 100% by mass)

[Comparative Formulation Example 1]
A lotion was produced in the same manner as in Formulation Example 1, except that 50% by mass of 1,3-butylene glycol was blended instead of the wrinkle-improving composition of Production Example 1.

[Test Example] Wrinkle-improving action test Using the lotions of Formulation Example 1 and Comparative Formulation Example 1 obtained as described above, wrinkles were reduced as follows in accordance with the guidelines (Non-Patent Documents 1 and 2). The ameliorative action was tested.

Twenty-three women (average: 50.00 years old, standard deviation: 1.86 years old) with similar wrinkles on the left and right corners of the eyes were selected as subjects. In addition, those who have factors (skin inflammation, eczema, trauma, etc.) on the skin of the evaluation site that may affect the test results; Subjects who had experience of collagen injection, photofacial, etc. were excluded from the subjects.

Each test subject applied the lotion of Formulation Example 1 on one side of the left and right corners of the eyes, and the lotion of Comparative Formulation Example 1 on the other side, twice a day in the morning and evening. It was applied for 4 weeks. Each subject was randomly assigned to apply both lotions on the left or right side.
On the test start day and 4 weeks after the start of the test, wrinkles present at the corners of the eyes were evaluated. In the evaluation, after washing the face, after acclimating for 15 minutes or more in an environmental test room (room temperature 20-24 ° C, humidity 45-55%), the target area of the outer corner of the eye on both sides was visually evaluated by an expert. Direct three-dimensional measurement of the wrinkles present in the

In the visual evaluation, two experts visually observed wrinkles at the corners of the eyes, and scored the condition of wrinkles based on the wrinkle grade standard photographs (grades 0 to 7) of the guidelines described above. When the state of wrinkles did not correspond to the standard photograph of each grade, the score was evaluated with the intermediate value and the 1/4 value.

In the three-dimensional analysis, the target regions of the corners of the eyes on both sides were measured using an optical three-dimensional measurement system for skin (PRIMOS, manufactured by GF Mestechnic) to obtain three-dimensional data. Using the obtained three-dimensional data, analysis software attached to PRIMOS was used to analyze the three-dimensional data on the start date of the test and four weeks after the start of the test by aligning the positions of the analysis range.
Wrinkle analysis is performed using wrinkle average depth, maximum wrinkle average depth, maximum wrinkle maximum depth, total wrinkle volume, Ra value (arithmetic mean roughness of roughness curve), and Ry value (maximum height of roughness curve). ) were evaluated using six parameters.

The obtained results were tested for normality by the Shapiro-Wilk test and kurtosis/skewness, the paired t-test was performed for the parametric test, and the Wilcoxon signed-rank test and Bonferroni correction were performed for the non-parametric test. Ta. For comparison between Formulation Example 1 and Comparative Formulation Example 1, RM ANOVA was performed using the amount of change after 4 weeks from the start date. In addition, in the visual evaluation, the analysis was performed on the cases where the intraclass correlation coefficient exceeded 0.8 between the evaluations by the two experts.
In each test, p<0.05 was considered significant. Also, the rate of change from the start date was calculated by the following formula.
Rate of change from start date (%) = (value after 4 weeks - value on start date) / value on start date x 100
The results are shown in Tables 2 and 3.

As shown in Table 2, in the wrinkle grade evaluation by visual judgment by experts, the wrinkle grade was significantly improved by applying the lotion of Formulation Example 1 for 4 weeks. In addition, when comparing the application site of Formulation Example 1 and the application site of Comparative Formulation Example 1, the amount of change after 4 weeks from the start of the test, the application site of Formulation Example 1 and the application site of Comparative Formulation Example 1 A significant difference was observed between

Furthermore, as shown in Table 3, even in the three-dimensional analysis, by applying the lotion of Formulation Example 1 for 4 weeks, the average wrinkle depth, the maximum average wrinkle depth, the maximum maximum wrinkle depth, the total wrinkle volume, Six items of Ra value (arithmetic mean roughness of roughness curve) and Ry value (maximum height of roughness curve) were significantly reduced. Then, when comparing Formulation Example 1 and Comparative Formulation Example 1 with respect to the above six items, a significant difference was observed between the application site of Formulation Example 1 and the application site of Comparative Formulation Example 1 in terms of the amount of change in 4 weeks. was taken.

From the above results, in the group applied with Formulation Example 1 (containing 5 kinds of natural product-derived ingredients), wrinkles at the corners of the eyes were improved in comparison before and after the test period, and in comparison with the group applied with Comparative Formulation Example 1. recognized as a thing.

[Formulation Example 2]
A milky lotion was prepared by a conventional method according to the following composition.
Wrinkle improvement composition (Production Example 1) 1.00 g
(Total amount of 5 kinds of natural product-derived ingredients: 0.0060 g)
Jojoba oil 4.00g
1,3-butylene glycol 3.00 g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
2.00 g olive oil
2.00 g of squalane
Cetanol 2.00g
Glyceryl monostearate 2.00g
Polyoxyethylene sorbitan oleate (20E.O.) 2.00g
Methyl paraoxybenzoate 0.15g
Huangji extract 0.10g
Ginkgo biloba extract 0.10g
Phellodendron bark extract 0.10g
Chamomile extract 0.10g
Perfume 0.05g
Remainder of purified water (total amount is 100 g)

[Formulation Example 3]
A cream having the following composition was produced by a conventional method.
Wrinkle improvement composition (Production Example 1) 4.00 g
(Total amount of 5 kinds of natural product-derived ingredients: 0.0240 g)
Kujin extract 0.1g
Scutellaria root extract 0.1g
Liquid paraffin 5.0g
Bleached beeswax 4.0g
Squalane 10.0g
Cetanol 3.0g
Lanolin 2.0g
1.0 g of stearic acid
Polyoxyethylene sorbitan oleate (20E.O.) 1.5g
Glyceryl monostearate 3.0g
1,3-butylene glycol 6.0 g
Methyl paraoxybenzoate 1.5g
Perfume 0.1g
Remainder of purified water (total amount is 100 g)

[Formulation Example 4]
A beauty essence having the following composition was prepared by a conventional method.
Wrinkle improvement composition (Production Example 1) 1.00 g
(Total amount of 5 kinds of natural product-derived ingredients: 0.0060 g)
Chamomile extract 0.1g
carrot extract 0.1g
xanthan gum 0.3g
0.1 g of hydroxyethyl cellulose
Carboxyvinyl polymer 0.1 g
1,3-butylene glycol 4.0 g
2.0 g of glycerin
Potassium hydroxide 0.25g
Perfume 0.01g
Preservative (methyl paraoxybenzoate) 0.15g
2.0 g of ethanol
Remainder of purified water (total amount is 100 g)

The anti-wrinkle composition of the present invention can greatly contribute to the improvement of skin aging symptoms caused by the formation of wrinkles.

Claims (1)

licorice leaf extract;
Combining four kinds of combinations of Angelica keiskei extract, Kinginka extract, Yokuinin extract and pearl protein hydrolyzate ,
The mass ratio of the angelica extract to 100 parts by mass of the licorice leaf extract is 30 parts by mass or more and 70 parts by mass or less,
The mass ratio of the snapper extract to 100 parts by mass of the licorice leaf extract is 5 parts by mass or more and 30 parts by mass or less,
The mass ratio of the Yokuinin extract to 100 parts by mass of the licorice leaf extract is 0.5 parts by mass or more and 10 parts by mass or less,
A mass ratio of the pearl protein hydrolyzate to 100 parts by mass of the licorice leaf extract is 3 parts by mass or more and 30 parts by mass or less.
A wrinkle-improving skin cosmetic containing 0.004 to 0.1% by mass of a wrinkle-improving composition.